AlfalfaGEDB | MS.gene27251

M. sativa cultivar XinJiangDaYe / MS.gene27251

KEGG: K13082
GO: GO:0003674 GO:0003824 GO:0005488 GO:0048037 GO:0050662
PFAM: PF01370.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27251.t1	XP_013466134.1	96.4	331	12	0	1	331	1	331	1.90E-185	658.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27251.t1	Q9XES5	73.6	326	86	0	1	326	1	326	1.2e-146	520.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27251.t1	Q6TQT1	96.4	331	12	0	1	331	1	331	1.4e-185	658.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049907	MS.gene27251	0.804911	1.72E-49	-1.69E-46
MS.gene050581	MS.gene27251	0.8084	3.15E-50	-1.69E-46
MS.gene056902	MS.gene27251	0.810478	1.13E-50	-1.69E-46
MS.gene056931	MS.gene27251	0.819477	1.14E-52	-1.69E-46
MS.gene056933	MS.gene27251	0.822986	1.77E-53	-1.69E-46
MS.gene056935	MS.gene27251	0.808229	3.43E-50	-1.69E-46
MS.gene059529	MS.gene27251	0.800741	1.25E-48	-1.69E-46
MS.gene059530	MS.gene27251	0.84975	2.65E-60	-1.69E-46
MS.gene060356	MS.gene27251	0.821897	3.16E-53	-1.69E-46
MS.gene060559	MS.gene27251	0.809644	1.71E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene50831	MS.gene27251	PPI
MS.gene27251	MS.gene50853	PPI
MS.gene27251	MS.gene058751	PPI
MS.gene058752	MS.gene27251	PPI
MS.gene27251	MS.gene009245	PPI
MS.gene27251	MS.gene50830	PPI
MS.gene50852	MS.gene27251	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27251.t1	MTR_1g022445	96.375	331	12	0	1	331	1	331	0.0	663
MS.gene27251.t1	MTR_1g022440	79.104	335	70	0	1	335	1	335	0.0	574
MS.gene27251.t1	MTR_7g074850	47.826	322	164	3	8	325	8	329	1.19e-102	305
MS.gene27251.t1	MTR_7g074820	47.516	322	165	3	8	325	8	329	2.01e-101	302
MS.gene27251.t1	MTR_7g074880	48.287	321	161	4	8	325	8	326	3.79e-101	301
MS.gene27251.t1	MTR_7g074730	45.794	321	171	3	8	325	8	328	8.56e-98	293
MS.gene27251.t1	MTR_4g092080	42.769	325	175	4	9	324	15	337	1.97e-94	284
MS.gene27251.t1	MTR_7g074710	44.548	321	173	4	8	325	8	326	3.17e-94	283
MS.gene27251.t1	MTR_4g023730	42.724	323	181	2	7	325	7	329	1.02e-91	277
MS.gene27251.t1	MTR_4g081440	41.935	341	164	8	9	326	21	350	2.78e-89	271
MS.gene27251.t1	MTR_4g081440	41.813	342	164	8	9	326	25	355	4.65e-89	271
MS.gene27251.t1	MTR_7g074870	45.614	285	151	3	43	325	6	288	6.00e-79	243
MS.gene27251.t1	MTR_3g005210	38.650	326	187	6	5	325	3	320	1.41e-77	241
MS.gene27251.t1	MTR_3g005170	38.037	326	189	6	2	322	4	321	2.42e-76	238
MS.gene27251.t1	MTR_8g062440	40.244	328	186	6	1	325	1	321	5.49e-75	234
MS.gene27251.t1	MTR_5g056600	39.688	320	185	6	9	325	5	319	1.08e-74	233
MS.gene27251.t1	MTR_2g101330	37.651	332	198	6	4	332	1	326	4.63e-74	232
MS.gene27251.t1	MTR_8g062440	40.122	329	186	7	1	325	1	322	3.95e-73	229
MS.gene27251.t1	MTR_8g062110	38.922	334	182	7	1	325	2	322	4.17e-73	229
MS.gene27251.t1	MTR_8g062110	38.922	334	182	7	1	325	11	331	6.27e-73	229
MS.gene27251.t1	MTR_3g005170	40.071	282	157	5	3	279	5	279	2.41e-72	226
MS.gene27251.t1	MTR_4g077130	37.963	324	192	5	2	322	3	320	8.42e-71	223
MS.gene27251.t1	MTR_8g062440	42.553	282	153	5	1	279	1	276	3.89e-70	222
MS.gene27251.t1	MTR_4g077100	38.941	321	187	6	5	322	8	322	1.10e-69	221
MS.gene27251.t1	MTR_4g006940	39.571	326	179	10	6	326	15	327	2.19e-67	215
MS.gene27251.t1	MTR_8g062110	40.278	288	151	6	1	279	2	277	2.88e-67	213
MS.gene27251.t1	MTR_8g062110	40.278	288	151	6	1	279	2	277	3.68e-67	213
MS.gene27251.t1	MTR_2g104960	40.123	324	180	10	5	325	17	329	8.22e-67	214
MS.gene27251.t1	MTR_4g077100	41.007	278	156	5	5	279	8	280	2.30e-66	211
MS.gene27251.t1	MTR_5g072620	35.866	329	194	8	4	325	1	319	1.39e-64	207
MS.gene27251.t1	MTR_3g031650	35.562	329	195	7	4	325	1	319	1.35e-63	204
MS.gene27251.t1	MTR_4g009040	36.335	322	190	10	8	325	13	323	2.97e-63	204
MS.gene27251.t1	MTR_4g009040	35.890	326	190	11	8	325	13	327	5.45e-61	198
MS.gene27251.t1	MTR_4g006940	41.007	278	148	8	6	279	15	280	3.11e-60	195
MS.gene27251.t1	MTR_4g006940	41.007	278	148	8	6	279	15	280	3.11e-60	195
MS.gene27251.t1	MTR_3g031650	37.500	280	159	6	4	276	1	271	1.72e-58	190
MS.gene27251.t1	MTR_4g009690	35.312	320	195	8	8	325	13	322	1.67e-57	189
MS.gene27251.t1	MTR_3g005170	33.129	326	174	7	2	322	4	290	1.89e-56	186
MS.gene27251.t1	MTR_4g009690	35.514	321	194	9	8	325	13	323	4.69e-56	185
MS.gene27251.t1	MTR_2g013230	34.524	336	199	7	8	325	8	340	5.47e-56	186
MS.gene27251.t1	MTR_2g013250	33.832	334	200	7	8	323	7	337	3.71e-54	181
MS.gene27251.t1	MTR_2g028620	33.030	330	200	10	6	325	8	326	1.73e-49	169
MS.gene27251.t1	MTR_2g013260	33.234	334	202	7	8	323	8	338	3.64e-49	168
MS.gene27251.t1	MTR_7g117190	31.250	336	211	7	8	325	7	340	1.93e-46	160
MS.gene27251.t1	MTR_2g028620	34.520	281	164	9	6	276	8	278	8.64e-45	156
MS.gene27251.t1	MTR_2g013260	34.138	290	172	6	8	279	8	296	4.03e-44	153
MS.gene27251.t1	MTR_7g117190	32.753	287	175	6	8	276	7	293	1.22e-41	147
MS.gene27251.t1	MTR_5g029990	29.712	313	184	11	5	305	9	297	7.66e-32	121
MS.gene27251.t1	MTR_8g062110	32.218	239	140	7	96	325	11	236	1.26e-31	119
MS.gene27251.t1	MTR_2g013230	31.276	243	146	7	101	325	11	250	9.56e-29	112
MS.gene27251.t1	MTR_0164s0070	40.000	130	77	1	4	132	1	130	1.40e-28	110
MS.gene27251.t1	MTR_8g062110	32.642	193	109	6	96	279	11	191	7.52e-26	102
MS.gene27251.t1	MTR_6g406250	29.297	256	161	10	8	253	55	300	1.12e-23	100
MS.gene27251.t1	MTR_2g013260	29.461	241	149	7	101	323	30	267	1.95e-21	92.8
MS.gene27251.t1	MTR_6g406250	29.245	212	140	6	8	213	55	262	1.08e-20	90.5
MS.gene27251.t1	MTR_3g101500	28.315	279	169	10	8	277	4	260	2.03e-18	84.7
MS.gene27251.t1	MTR_3g101500	28.315	279	169	10	8	277	4	260	3.04e-18	84.7
MS.gene27251.t1	MTR_7g117190	26.957	230	148	7	114	325	3	230	6.31e-18	82.0
MS.gene27251.t1	MTR_0685s0030	27.778	162	113	3	161	322	2	159	8.36e-14	68.9
MS.gene27251.t1	MTR_3g011130	34.711	121	68	5	99	213	30	145	1.61e-13	67.4
MS.gene27251.t1	MTR_3g101500	29.474	190	113	6	8	196	4	173	1.10e-12	67.0
MS.gene27251.t1	MTR_0685s0030	28.205	117	82	1	163	279	3	117	8.92e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27251.t1	AT5G42800	69.325	326	100	0	1	326	1	326	2.72e-176	494
MS.gene27251.t1	AT4G35420	42.500	320	176	6	8	325	8	321	9.62e-89	270
MS.gene27251.t1	AT2G45400	43.077	325	178	3	8	325	40	364	2.18e-87	267
MS.gene27251.t1	AT1G61720	42.342	333	175	5	5	325	10	337	1.06e-86	265
MS.gene27251.t1	AT4G27250	38.393	336	181	9	3	317	8	338	5.57e-79	245
MS.gene27251.t1	AT1G09510	42.073	328	181	5	1	325	1	322	2.72e-78	243
MS.gene27251.t1	AT4G27250	38.602	329	180	8	3	317	8	328	1.68e-77	241
MS.gene27251.t1	AT1G51410	38.700	323	189	5	6	325	7	323	3.67e-77	240
MS.gene27251.t1	AT2G45400	43.885	278	149	3	8	278	40	317	5.86e-76	236
MS.gene27251.t1	AT5G19440	39.692	325	183	6	5	324	7	323	1.38e-72	228
MS.gene27251.t1	AT1G66800	39.198	324	184	8	1	319	1	316	4.02e-70	221
MS.gene27251.t1	AT2G45400	40.345	290	168	2	41	325	18	307	2.52e-69	219
MS.gene27251.t1	AT1G68540	38.629	321	187	7	10	325	6	321	7.27e-69	218
MS.gene27251.t1	AT1G09490	38.279	337	181	8	1	325	1	322	6.33e-68	216
MS.gene27251.t1	AT1G09480	38.253	332	188	8	1	325	1	322	1.46e-65	210
MS.gene27251.t1	AT1G15950	38.889	324	184	10	5	325	10	322	1.73e-65	210
MS.gene27251.t1	AT1G15950	38.889	324	184	10	5	325	10	322	1.73e-65	210
MS.gene27251.t1	AT2G33590	36.449	321	192	8	8	325	9	320	4.07e-65	209
MS.gene27251.t1	AT1G09480	38.253	332	188	8	1	325	48	369	4.24e-65	210
MS.gene27251.t1	AT1G25460	37.879	330	186	9	4	325	1	319	1.43e-64	207
MS.gene27251.t1	AT1G15950	39.228	311	176	9	5	312	10	310	9.94e-64	206
MS.gene27251.t1	AT2G02400	36.556	331	183	11	6	325	4	318	1.15e-63	205
MS.gene27251.t1	AT1G80820	38.820	322	183	10	8	326	8	318	2.18e-63	204
MS.gene27251.t1	AT5G58490	35.168	327	195	7	6	325	7	323	4.92e-63	203
MS.gene27251.t1	AT1G09500	36.418	335	193	8	1	325	1	325	7.62e-63	203
MS.gene27251.t1	AT1G09500	38.328	287	160	6	1	279	1	278	1.57e-61	198
MS.gene27251.t1	AT2G33600	35.312	320	197	7	8	325	9	320	3.32e-59	193
MS.gene27251.t1	AT2G45400	46.759	216	108	3	8	216	40	255	1.27e-57	187
MS.gene27251.t1	AT1G15950	40.072	277	154	8	5	279	10	276	2.21e-57	187
MS.gene27251.t1	AT1G15950	39.643	280	157	8	5	282	10	279	2.30e-57	188
MS.gene27251.t1	AT1G15950	39.711	277	155	8	5	279	10	276	1.49e-56	185
MS.gene27251.t1	AT1G80820	39.416	274	154	8	8	279	8	271	1.16e-54	180
MS.gene27251.t1	AT1G76470	33.028	327	213	6	3	327	2	324	5.16e-54	180
MS.gene27251.t1	AT1G09490	35.570	298	165	8	40	325	9	291	3.23e-49	167
MS.gene27251.t1	AT1G09500	34.007	297	176	8	39	325	5	291	2.46e-45	156
MS.gene27251.t1	AT1G68540	35.616	219	132	6	111	325	1	214	2.64e-35	128
MS.gene27251.t1	AT1G15950	34.783	230	141	7	99	325	1	224	9.61e-35	127
MS.gene27251.t1	AT4G30470	28.223	287	172	12	9	282	11	276	1.43e-28	112
MS.gene27251.t1	AT2G23910	26.786	280	184	11	9	281	11	276	1.86e-27	109
MS.gene27251.t1	AT2G23910	26.978	278	182	11	9	279	11	274	2.81e-27	108
MS.gene27251.t1	AT2G23910	26.578	301	198	12	9	300	11	297	3.07e-27	109
MS.gene27251.t1	AT4G33360	28.623	276	172	8	8	277	15	271	4.54e-22	95.1
MS.gene27251.t1	AT5G14700	29.771	262	157	10	8	253	55	305	6.09e-22	95.5
MS.gene27251.t1	AT4G33360	28.623	276	172	8	8	277	3	259	6.95e-22	94.7
MS.gene27251.t1	AT4G33360	28.623	276	172	8	8	277	15	271	7.92e-22	95.1
MS.gene27251.t1	AT5G14700	31.000	200	122	6	8	196	55	249	7.32e-19	85.9
MS.gene27251.t1	AT2G23910	25.385	260	171	12	50	300	18	263	6.46e-18	82.8

Find 85 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGATCTTCAGTAACGTTT+AGG	0.241553	1.1:-1764189	None:intergenic
ATTGATACATGCAGAGAAAA+AGG	0.247887	1.1:+1765183	MS.gene27251:CDS
ATTGGTGCTTTAACAAATTC+AGG	0.252064	1.1:-1765213	None:intergenic
TTCAGTAACGTTTAGGGTTC+CGG	0.269534	1.1:-1764182	None:intergenic
ATGATCAAGCCTACCATAAA+AGG	0.270848	1.1:+1764091	MS.gene27251:CDS
CATATTCTTGTTTGAGCATA+TGG	0.271150	1.1:+1764852	MS.gene27251:CDS
TGTTTGCGTCACAGGGGCTT+CGG	0.316160	1.1:+1761767	MS.gene27251:CDS
GATCATCCAAGTGGACAAAT+TGG	0.337508	1.1:-1764816	None:intergenic
TTGATCTTCAGTAACGTTTA+GGG	0.340589	1.1:-1764188	None:intergenic
TTTGAGCATATGGAAGTAGA+AGG	0.341082	1.1:+1764862	MS.gene27251:CDS
GAAGTCCATCTTGTGCTCTT+TGG	0.342018	1.1:-1764471	None:intergenic
GCCGAAACTGTTTGCGTCAC+AGG	0.351008	1.1:+1761759	MS.gene27251:CDS
TGTGTGACACCATTACTTAT+TGG	0.352169	1.1:-1765231	None:intergenic
AATCAATCTACGGACAGTTT+TGG	0.354166	1.1:-1764146	None:intergenic
CAAGAATATTCCAGATCAAT+TGG	0.355555	1.1:+1765077	MS.gene27251:CDS
GTTTGCGTCACAGGGGCTTC+GGG	0.370303	1.1:+1761768	MS.gene27251:CDS
CCCTGTGACGCAAACAGTTT+CGG	0.380229	1.1:-1761760	None:intergenic
CTTCGGGTTTCATCGGGTCA+TGG	0.381174	1.1:+1761784	MS.gene27251:CDS
CTCACAAGCTCCAATTGATC+TGG	0.389786	1.1:-1765087	None:intergenic
ACAGGGGCTTCGGGTTTCAT+CGG	0.391119	1.1:+1761777	MS.gene27251:CDS
ATCATCCCACCTCTTGTTGT+TGG	0.405200	1.1:+1764500	MS.gene27251:CDS
TTTGTGGGGATATTGTACTC+TGG	0.409401	1.1:-1764952	None:intergenic
TTTATAAATGTACCAGTGAT+AGG	0.410676	1.1:-1764572	None:intergenic
CAAAGAGCAAACTGTCCATT+TGG	0.411352	1.1:+1762100	MS.gene27251:CDS
CAGGGGCTTCGGGTTTCATC+GGG	0.419287	1.1:+1761778	MS.gene27251:CDS
GCATTAAGTACTTACTTTGT+GGG	0.426047	1.1:-1764967	None:intergenic
TTGTGGGGATATTGTACTCT+GGG	0.449070	1.1:-1764951	None:intergenic
GAAGCAATTAAAGGGTGTAC+TGG	0.456664	1.1:+1762156	MS.gene27251:CDS
TATGGGTACCTCAGGGTCCT+TGG	0.456797	1.1:-1762214	None:intergenic
GACAGTTTGCTCTTTGCACC+TGG	0.475948	1.1:-1762093	None:intergenic
GCAAGGCCAATTTGTCCACT+TGG	0.475951	1.1:+1764810	MS.gene27251:CDS
TAGGAGAAAGGGCAGTGATT+AGG	0.477149	1.1:-1764553	None:intergenic
ATCAAAGAAGATCAAAGACT+TGG	0.477770	1.1:+1765116	MS.gene27251:CDS
GGCATAGTTGGAATAAGAAA+AGG	0.483517	1.1:-1764524	None:intergenic
GCACAAATTTGCAGGAAATG+AGG	0.483996	1.1:+1764768	MS.gene27251:intron
ATTAGGCTAGGTGGCATAGT+TGG	0.487275	1.1:-1764536	None:intergenic
TTGAGCATATGGAAGTAGAA+GGG	0.498318	1.1:+1764863	MS.gene27251:CDS
CCGAAACTGTTTGCGTCACA+GGG	0.498950	1.1:+1761760	MS.gene27251:CDS
AGTCCTTTGTTAGAACTGCC+AGG	0.502761	1.1:+1762075	MS.gene27251:CDS
AAAATAAAATATGGGTTCCA+TGG	0.504913	1.1:+1761737	None:intergenic
ACTCATGGAGCGCGGTTACA+TGG	0.507446	1.1:+1761818	MS.gene27251:CDS
ATTTGAATTCAAATACAGCT+TGG	0.508167	1.1:+1765140	MS.gene27251:CDS
ATGTACCAGTGATAGGAGAA+AGG	0.508607	1.1:-1764565	None:intergenic
GAATGCAGAAAACTTGAAGA+AGG	0.510219	1.1:+1762050	MS.gene27251:intron
TGTACCAGTGATAGGAGAAA+GGG	0.510873	1.1:-1764564	None:intergenic
TTGATTTACACATCGTCCGC+CGG	0.512560	1.1:+1764163	MS.gene27251:CDS
GTTAAAGCACCAATAAGTAA+TGG	0.513064	1.1:+1765222	MS.gene27251:CDS
ACCAAGGTCAGCCTTCCAAA+TGG	0.516767	1.1:-1762115	None:intergenic
TGAAGATCAAAAGCCCTTGT+GGG	0.521869	1.1:+1764201	MS.gene27251:CDS
TCCATTTGGAAGGCTGACCT+TGG	0.530988	1.1:+1762114	MS.gene27251:CDS
AAGGCTGACCTTGGTGAAGA+AGG	0.534105	1.1:+1762123	MS.gene27251:CDS
TGAATTCAAATACAGCTTGG+AGG	0.534333	1.1:+1765143	MS.gene27251:CDS
CTGAAGATCAAAAGCCCTTG+TGG	0.543581	1.1:+1764200	MS.gene27251:CDS
AATAAAAGGGACCTGGGTCG+CGG	0.549676	1.1:-1761854	None:intergenic
ATGGCTTGTCATGAGACTCA+TGG	0.550625	1.1:+1761803	MS.gene27251:CDS
GACGCAAACAGTTTCGGCCA+TGG	0.555179	1.1:-1761754	None:intergenic
GGAGAGTGAAGATGACTGGC+TGG	0.556468	1.1:+1764257	MS.gene27251:CDS
CATTAAGTACTTACTTTGTG+GGG	0.557405	1.1:-1764966	None:intergenic
CGAGCAACAGTCCGCGACCC+AGG	0.559232	1.1:+1761843	MS.gene27251:CDS
CACTTGCGGAGCAAGAAGCA+TGG	0.562977	1.1:+1764441	MS.gene27251:CDS
TGCAGGAGAGTGAAGATGAC+TGG	0.565963	1.1:+1764253	MS.gene27251:CDS
TCAAAGAAGATCAAAGACTT+GGG	0.567744	1.1:+1765117	MS.gene27251:CDS
GAAAGGGCAGTGATTAGGCT+AGG	0.570840	1.1:-1764548	None:intergenic
AAAGGACCAACAACAAGAGG+TGG	0.582880	1.1:-1764506	None:intergenic
ACTGCCCTTTCTCCTATCAC+TGG	0.583168	1.1:+1764560	MS.gene27251:CDS
GAGCAAACTGTCCATTTGGA+AGG	0.583763	1.1:+1762104	MS.gene27251:CDS
ATTTGCCAAAGAGCACAAGA+TGG	0.605063	1.1:+1764466	MS.gene27251:CDS
AGAAAAGGACCAACAACAAG+AGG	0.606682	1.1:-1764509	None:intergenic
ACGATGTGTAAATCAATCTA+CGG	0.609135	1.1:-1764156	None:intergenic
CATTATTCGATTATAAAGCA+AGG	0.613434	1.1:+1764793	MS.gene27251:CDS
GAGAGTGAAGATGACTGGCT+GGG	0.614181	1.1:+1764258	MS.gene27251:CDS
AGCATATGGAAGTAGAAGGG+AGG	0.617663	1.1:+1764866	MS.gene27251:CDS
CCAACAGCTCTCATCCCACA+AGG	0.627961	1.1:-1764215	None:intergenic
CATCATGAAAGCATGCCTCA+AGG	0.630278	1.1:+1764123	MS.gene27251:CDS
TCCTTGGACTCAAAATCCAT+AGG	0.646498	1.1:-1762198	None:intergenic
CCTTGTGGGATGAGAGCTGT+TGG	0.652347	1.1:+1764215	MS.gene27251:CDS
AAGGACCAACAACAAGAGGT+GGG	0.653580	1.1:-1764505	None:intergenic
GCACCTGGCAGTTCTAACAA+AGG	0.664303	1.1:-1762078	None:intergenic
AGGGCAGTGATTAGGCTAGG+TGG	0.683144	1.1:-1764545	None:intergenic
CTTCACAAAGATCATCCAAG+TGG	0.692415	1.1:-1764825	None:intergenic
CAACAGCTCTCATCCCACAA+GGG	0.696587	1.1:-1764214	None:intergenic
CGAAACTGTTTGCGTCACAG+GGG	0.700520	1.1:+1761761	MS.gene27251:CDS
GTCATGAGACTCATGGAGCG+CGG	0.701087	1.1:+1761810	MS.gene27251:CDS
CAAAACTACCTTCTTCACCA+AGG	0.706268	1.1:-1762131	None:intergenic
AGTAACGTTTAGGGTTCCGG+CGG	0.708151	1.1:-1764179	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATAATAAAAAATCAGTAAA+AGG	-	chr1.1:1763816-1763835	None:intergenic	10.0%
!!	TTAAATTATAAAACTCTTAT+AGG	-	chr1.1:1764334-1764353	None:intergenic	10.0%
!!	TTTAATACCATTATATATAT+TGG	-	chr1.1:1762490-1762509	None:intergenic	10.0%
!!!	AATCTAATCATTTTTTTTTT+TGG	+	chr1.1:1763718-1763737	MS.gene27251:intron	10.0%
!!!	ATAAAAGTGAATTAAAATTT+TGG	+	chr1.1:1763345-1763364	MS.gene27251:intron	10.0%
!!!	ATTTTGATTAAAAAAATTGT+TGG	-	chr1.1:1761932-1761951	None:intergenic	10.0%
!!!	TAATTTAATTTAAAGTGATT+AGG	-	chr1.1:1764363-1764382	None:intergenic	10.0%
!!!	TTTTTATAAACTTTAGTATA+TGG	-	chr1.1:1763612-1763631	None:intergenic	10.0%
!!	AAAAAAATGATTAGATTGTT+AGG	-	chr1.1:1763715-1763734	None:intergenic	15.0%
!!	AAAACTATAACTGAAAATTA+TGG	-	chr1.1:1763277-1763296	None:intergenic	15.0%
!!	ATGCATATAAAATTAAAGTA+TGG	-	chr1.1:1762235-1762254	None:intergenic	15.0%
!!	CATTAATCCAATATATATAA+TGG	+	chr1.1:1762480-1762499	MS.gene27251:intron	15.0%
!!	CATTATATATATTGGATTAA+TGG	-	chr1.1:1762482-1762501	None:intergenic	15.0%
!!	TGCATATAAAATTAAAGTAT+GGG	-	chr1.1:1762234-1762253	None:intergenic	15.0%
!!!	AATTAAAATTTTGGTGATAA+TGG	+	chr1.1:1763354-1763373	MS.gene27251:intron	15.0%
!!!	ATAGATATCTAAGTTTTTTT+TGG	+	chr1.1:1763027-1763046	MS.gene27251:intron	15.0%
!!!	ATTGTTTAAATTGGATTTTT+AGG	+	chr1.1:1765052-1765071	MS.gene27251:intron	15.0%
!!!	CCAAAATTAGAAATTTTTTA+TGG	+	chr1.1:1763090-1763109	MS.gene27251:intron	15.0%
!!!	CCATAAAAAATTTCTAATTT+TGG	-	chr1.1:1763093-1763112	None:intergenic	15.0%
!!!	TAAGGAATTTAAAATAGAAA+TGG	-	chr1.1:1763168-1763187	None:intergenic	15.0%
!!!	TAGATATCTAAGTTTTTTTT+GGG	+	chr1.1:1763028-1763047	MS.gene27251:intron	15.0%
!!!	TATAGTTTGATTTGTAATTT+TGG	-	chr1.1:1763651-1763670	None:intergenic	15.0%
!!	AGCATAATGTAATTTATTAG+GGG	+	chr1.1:1762767-1762786	MS.gene27251:intron	20.0%
!!	ATCTCTTGTGAAAAATTTAT+TGG	+	chr1.1:1763773-1763792	MS.gene27251:intron	20.0%
!!	GAGCATAATGTAATTTATTA+GGG	+	chr1.1:1762766-1762785	MS.gene27251:intron	20.0%
!!	GCTTAAATTCCTTAAAAAAT+TGG	+	chr1.1:1764637-1764656	MS.gene27251:intron	20.0%
!!	GTTATTATCAATGTAGTAAT+GGG	-	chr1.1:1762419-1762438	None:intergenic	20.0%
!!	GTTGCAACATAAAAATAAAA+GGG	-	chr1.1:1761870-1761889	None:intergenic	20.0%
!!	TGAGCATAATGTAATTTATT+AGG	+	chr1.1:1762765-1762784	MS.gene27251:intron	20.0%
!!	TGTTGCAACATAAAAATAAA+AGG	-	chr1.1:1761871-1761890	None:intergenic	20.0%
!!	TTATAAACTTTAGTATATGG+TGG	-	chr1.1:1763609-1763628	None:intergenic	20.0%
!!!	GAATCATTTCCAATTTTTTA+AGG	-	chr1.1:1764649-1764668	None:intergenic	20.0%
!!!	GTCACAACTATTTTTTTAAA+GGG	+	chr1.1:1762980-1762999	MS.gene27251:intron	20.0%
!!!	GTTTATATATACACACATTT+TGG	+	chr1.1:1762028-1762047	MS.gene27251:intron	20.0%
!!!	TGTCACAACTATTTTTTTAA+AGG	+	chr1.1:1762979-1762998	MS.gene27251:intron	20.0%
!!!	TTACGTAGATATTTAAAACT+TGG	-	chr1.1:1763223-1763242	None:intergenic	20.0%
!	AAGTAAAACAAATGGTTGAT+TGG	+	chr1.1:1763385-1763404	MS.gene27251:intron	25.0%
!	AATACATTCAGTAGAAATCA+AGG	+	chr1.1:1762693-1762712	MS.gene27251:intron	25.0%
!	ACTTCAAATGTTTAATTGAG+AGG	+	chr1.1:1762854-1762873	MS.gene27251:intron	25.0%
!	AGTAACTACTAAAATGGAAA+CGG	-	chr1.1:1762457-1762476	None:intergenic	25.0%
!	ATACATTCAGTAGAAATCAA+GGG	+	chr1.1:1762694-1762713	MS.gene27251:intron	25.0%
!	ATTTGAATTCAAATACAGCT+TGG	+	chr1.1:1765140-1765159	MS.gene27251:CDS	25.0%
!	CAAAACTCAAGAAAATTGAT+GGG	+	chr1.1:1762006-1762025	MS.gene27251:intron	25.0%
!	CATTATTCGATTATAAAGCA+AGG	+	chr1.1:1764793-1764812	MS.gene27251:CDS	25.0%
!	GCTTGTCTAATTGTTTAAAT+TGG	+	chr1.1:1765043-1765062	MS.gene27251:intron	25.0%
!	GGTTATTATCAATGTAGTAA+TGG	-	chr1.1:1762420-1762439	None:intergenic	25.0%
!	TCAAAACTCAAGAAAATTGA+TGG	+	chr1.1:1762005-1762024	MS.gene27251:intron	25.0%
!	TCTATCATCTCATATATGAA+TGG	+	chr1.1:1762254-1762273	MS.gene27251:intron	25.0%
!	TTTATAAATGTACCAGTGAT+AGG	-	chr1.1:1764575-1764594	None:intergenic	25.0%
!!	AATTGTTACTTAGTAGTAGA+CGG	-	chr1.1:1764727-1764746	None:intergenic	25.0%
!!!	AGTTTTGATGAAGCAATTAA+AGG	+	chr1.1:1762147-1762166	MS.gene27251:CDS	25.0%
!!!	GTTTTGATGAAGCAATTAAA+GGG	+	chr1.1:1762148-1762167	MS.gene27251:CDS	25.0%
!!!	TAAAATTTTGGTGATAATGG+TGG	+	chr1.1:1763357-1763376	MS.gene27251:intron	25.0%
	AACCTAGTATAACATCCTTT+GGG	+	chr1.1:1762363-1762382	MS.gene27251:intron	30.0%
	AAGGGTTAATGACAATGATT+TGG	+	chr1.1:1762998-1763017	MS.gene27251:intron	30.0%
	AATAATGCTAGTTATCCTGA+TGG	-	chr1.1:1762743-1762762	None:intergenic	30.0%
	AATAGCCTCTTCATTATTCA+AGG	+	chr1.1:1763435-1763454	MS.gene27251:intron	30.0%
	AATTCCTTAACATATGCCTT+TGG	+	chr1.1:1763179-1763198	MS.gene27251:intron	30.0%
	ACAATTGATCGAAGATAACA+TGG	+	chr1.1:1764741-1764760	MS.gene27251:intron	30.0%
	ACATAAAAATAAAAGGGACC+TGG	-	chr1.1:1761864-1761883	None:intergenic	30.0%
	ACGATGTGTAAATCAATCTA+CGG	-	chr1.1:1764159-1764178	None:intergenic	30.0%
	AGCATTAAGTACTTACTTTG+TGG	-	chr1.1:1764971-1764990	None:intergenic	30.0%
	AGGGTTAATGACAATGATTT+GGG	+	chr1.1:1762999-1763018	MS.gene27251:intron	30.0%
	AGGTGTATAACAATATGGTT+GGG	+	chr1.1:1764020-1764039	MS.gene27251:intron	30.0%
	AGTACAGGTGTATAACAATA+TGG	+	chr1.1:1764015-1764034	MS.gene27251:intron	30.0%
	AGTAGTGGTGTATAACAATA+TGG	+	chr1.1:1763957-1763976	MS.gene27251:intron	30.0%
	ATAGCCTCTTCATTATTCAA+GGG	+	chr1.1:1763436-1763455	MS.gene27251:intron	30.0%
	ATATATTTATAGCTACCCAG+TGG	-	chr1.1:1763913-1763932	None:intergenic	30.0%
	ATCAAAGAAGATCAAAGACT+TGG	+	chr1.1:1765116-1765135	MS.gene27251:CDS	30.0%
	ATGGTGAGTAACTACTAAAA+TGG	-	chr1.1:1762463-1762482	None:intergenic	30.0%
	ATGTAATTTATTAGGGGTGA+TGG	+	chr1.1:1762773-1762792	MS.gene27251:intron	30.0%
	ATTCCTTAACATATGCCTTT+GGG	+	chr1.1:1763180-1763199	MS.gene27251:intron	30.0%
	ATTGATACATGCAGAGAAAA+AGG	+	chr1.1:1765183-1765202	MS.gene27251:CDS	30.0%
	CAAGAATATTCCAGATCAAT+TGG	+	chr1.1:1765077-1765096	MS.gene27251:CDS	30.0%
	CATAAAAATAAAAGGGACCT+GGG	-	chr1.1:1761863-1761882	None:intergenic	30.0%
	CATTAAGTACTTACTTTGTG+GGG	-	chr1.1:1764969-1764988	None:intergenic	30.0%
	GAAAATGATAAACATCCCAA+AGG	-	chr1.1:1762381-1762400	None:intergenic	30.0%
	GAAACGGTTCATACATTTAA+TGG	-	chr1.1:1762441-1762460	None:intergenic	30.0%
	GAACACAAATAGAACAAAGT+TGG	-	chr1.1:1762895-1762914	None:intergenic	30.0%
	GCATTAAGTACTTACTTTGT+GGG	-	chr1.1:1764970-1764989	None:intergenic	30.0%
	TAAAACAAATGGTTGATTGG+TGG	+	chr1.1:1763388-1763407	MS.gene27251:intron	30.0%
	TAATGGGATGGTTACAAAAA+AGG	-	chr1.1:1762403-1762422	None:intergenic	30.0%
	TATATTTATAGCTACCCAGT+GGG	-	chr1.1:1763912-1763931	None:intergenic	30.0%
	TCAAAGAAGATCAAAGACTT+GGG	+	chr1.1:1765117-1765136	MS.gene27251:CDS	30.0%
	TGGTTGAGAAGTAAAACAAA+TGG	+	chr1.1:1763377-1763396	MS.gene27251:intron	30.0%
	TTCAACATCATCTAGTGATA+AGG	+	chr1.1:1762912-1762931	MS.gene27251:intron	30.0%
	TTGATACATGCAGAGAAAAA+GGG	+	chr1.1:1765184-1765203	MS.gene27251:CDS	30.0%
	TTGATCTTCAGTAACGTTTA+GGG	-	chr1.1:1764191-1764210	None:intergenic	30.0%
	TTTGATCTTCAGTAACGTTT+AGG	-	chr1.1:1764192-1764211	None:intergenic	30.0%
!	ATTGGTGCTTTAACAAATTC+AGG	-	chr1.1:1765216-1765235	None:intergenic	30.0%
!	CATATTCTTGTTTGAGCATA+TGG	+	chr1.1:1764852-1764871	MS.gene27251:CDS	30.0%
!	GGAAATTCAATATTTTGCGA+GGG	-	chr1.1:1762307-1762326	None:intergenic	30.0%
!	GTTAAAGCACCAATAAGTAA+TGG	+	chr1.1:1765222-1765241	MS.gene27251:CDS	30.0%
!	TGGAAATTCAATATTTTGCG+AGG	-	chr1.1:1762308-1762327	None:intergenic	30.0%
!	TTATCAATGTAGTAATGGGA+TGG	-	chr1.1:1762415-1762434	None:intergenic	30.0%
!!	ATGGTTTGTTAGATTCTCTA+TGG	+	chr1.1:1762792-1762811	MS.gene27251:intron	30.0%
!!!	TTTTGTTTCTAAGACACTTG+CGG	+	chr1.1:1764427-1764446	MS.gene27251:CDS	30.0%
	AATCCCCTTGAATAATGAAG+AGG	-	chr1.1:1763561-1763580	None:intergenic	35.0%
	AATTAAAGTATGGGTACCTC+AGG	-	chr1.1:1762225-1762244	None:intergenic	35.0%
	ATCCCAAAGGATGTTATACT+AGG	-	chr1.1:1762368-1762387	None:intergenic	35.0%
	ATGATCAAGCCTACCATAAA+AGG	+	chr1.1:1764091-1764110	MS.gene27251:CDS	35.0%
	ATTAAAGTATGGGTACCTCA+GGG	-	chr1.1:1762224-1762243	None:intergenic	35.0%
	CAACCTAGTATAACATCCTT+TGG	+	chr1.1:1762362-1762381	MS.gene27251:intron	35.0%
	CACTATGAAGCAATGTTCTT+TGG	-	chr1.1:1762328-1762347	None:intergenic	35.0%
	CAGGTGTATAACAATATGGT+TGG	+	chr1.1:1764019-1764038	MS.gene27251:intron	35.0%
	CTACGTAAATGAGATTGAGA+TGG	+	chr1.1:1763235-1763254	MS.gene27251:intron	35.0%
	GAATGCAGAAAACTTGAAGA+AGG	+	chr1.1:1762050-1762069	MS.gene27251:intron	35.0%
	GGCATAGTTGGAATAAGAAA+AGG	-	chr1.1:1764527-1764546	None:intergenic	35.0%
	TAGCCTCTTCATTATTCAAG+GGG	+	chr1.1:1763437-1763456	MS.gene27251:intron	35.0%
	TAGTGATAAGGACAGTGAAT+AGG	+	chr1.1:1762924-1762943	MS.gene27251:intron	35.0%
	TGAATTCAAATACAGCTTGG+AGG	+	chr1.1:1765143-1765162	MS.gene27251:CDS	35.0%
	TGTGTGACACCATTACTTAT+TGG	-	chr1.1:1765234-1765253	None:intergenic	35.0%
	TTCCTTAACATATGCCTTTG+GGG	+	chr1.1:1763181-1763200	MS.gene27251:intron	35.0%
	TTGAGCATATGGAAGTAGAA+GGG	+	chr1.1:1764863-1764882	MS.gene27251:CDS	35.0%
	TTGTTCCTTCCACGATTTAA+GGG	+	chr1.1:1762627-1762646	MS.gene27251:intron	35.0%
	TTTGAGCATATGGAAGTAGA+AGG	+	chr1.1:1764862-1764881	MS.gene27251:CDS	35.0%
	TTTGTTCCTTCCACGATTTA+AGG	+	chr1.1:1762626-1762645	MS.gene27251:intron	35.0%
!	AATCAATCTACGGACAGTTT+TGG	-	chr1.1:1764149-1764168	None:intergenic	35.0%
!	GTTTGTTAGATTCTCTATGG+TGG	+	chr1.1:1762795-1762814	MS.gene27251:intron	35.0%
!!	ATGGTTTTGCACAAATTTGC+AGG	+	chr1.1:1764760-1764779	MS.gene27251:intron	35.0%
	AATGTTAACATGTGCCCCAA+AGG	-	chr1.1:1763198-1763217	None:intergenic	40.0%
	ACAAGAGATGATCAGTGTTC+AGG	-	chr1.1:1763762-1763781	None:intergenic	40.0%
	AGAAAAGGACCAACAACAAG+AGG	-	chr1.1:1764512-1764531	None:intergenic	40.0%
	ATGTACCAGTGATAGGAGAA+AGG	-	chr1.1:1764568-1764587	None:intergenic	40.0%
	CAAAACTACCTTCTTCACCA+AGG	-	chr1.1:1762134-1762153	None:intergenic	40.0%
	CAAAGAGCAAACTGTCCATT+TGG	+	chr1.1:1762100-1762119	MS.gene27251:CDS	40.0%
	CATCCCCTTGAATAATGAAG+AGG	-	chr1.1:1763443-1763462	None:intergenic	40.0%
	CTGCTGCAAAAAGAAATGTG+TGG	+	chr1.1:1763843-1763862	MS.gene27251:intron	40.0%
	CTTCACAAAGATCATCCAAG+TGG	-	chr1.1:1764828-1764847	None:intergenic	40.0%
	GATCATCCAAGTGGACAAAT+TGG	-	chr1.1:1764819-1764838	None:intergenic	40.0%
	GCACAAATTTGCAGGAAATG+AGG	+	chr1.1:1764768-1764787	MS.gene27251:intron	40.0%
	TAAATTAGTCGTGATCCCGT+CGG	-	chr1.1:1762594-1762613	None:intergenic	40.0%
	TCCTTGGACTCAAAATCCAT+AGG	-	chr1.1:1762201-1762220	None:intergenic	40.0%
	TGAAGATCAAAAGCCCTTGT+GGG	+	chr1.1:1764201-1764220	MS.gene27251:CDS	40.0%
	TGTACCAGTGATAGGAGAAA+GGG	-	chr1.1:1764567-1764586	None:intergenic	40.0%
	TTGTGCACGATCTTAAGTAG+TGG	+	chr1.1:1763942-1763961	MS.gene27251:intron	40.0%
	TTGTGGGGATATTGTACTCT+GGG	-	chr1.1:1764954-1764973	None:intergenic	40.0%
	TTTGTGGGGATATTGTACTC+TGG	-	chr1.1:1764955-1764974	None:intergenic	40.0%
!	ATTTCGCTAGCTTATCCATC+AGG	+	chr1.1:1762725-1762744	MS.gene27251:intron	40.0%
!	ATTTGCCAAAGAGCACAAGA+TGG	+	chr1.1:1764466-1764485	MS.gene27251:CDS	40.0%
!	CTTGTTATCTTGTGCCCTTA+GGG	+	chr1.1:1763052-1763071	MS.gene27251:intron	40.0%
!	GAAGCAATTAAAGGGTGTAC+TGG	+	chr1.1:1762156-1762175	MS.gene27251:CDS	40.0%
!	GATGTCTAGCACTCCTTTTA+TGG	-	chr1.1:1764107-1764126	None:intergenic	40.0%
!	GTGTACTGGAGTTTTTCATG+TGG	+	chr1.1:1762170-1762189	MS.gene27251:CDS	40.0%
!	TCTAGCACTCCTTTTATGGT+AGG	-	chr1.1:1764103-1764122	None:intergenic	40.0%
!	TCTTGTTATCTTGTGCCCTT+AGG	+	chr1.1:1763051-1763070	MS.gene27251:intron	40.0%
!	TGTTACTTAGTAGTAGACGG+TGG	-	chr1.1:1764724-1764743	None:intergenic	40.0%
!	TTCAGTAACGTTTAGGGTTC+CGG	-	chr1.1:1764185-1764204	None:intergenic	40.0%
!	TTTTCATGTGGCTACACCTA+TGG	+	chr1.1:1762182-1762201	MS.gene27251:CDS	40.0%
!!	ACCTATGGATTTTGAGTCCA+AGG	+	chr1.1:1762197-1762216	MS.gene27251:CDS	40.0%
!!	ATTCCTTAACTTGTGCCCTA+AGG	-	chr1.1:1763070-1763089	None:intergenic	40.0%
!!	TTCCTTAACTTGTGCCCTAA+GGG	-	chr1.1:1763069-1763088	None:intergenic	40.0%
	AAAGGACCAACAACAAGAGG+TGG	-	chr1.1:1764509-1764528	None:intergenic	45.0%
	AAATTAGTCGTGATCCCGTC+GGG	-	chr1.1:1762593-1762612	None:intergenic	45.0%
	AAGGACCAACAACAAGAGGT+GGG	-	chr1.1:1764508-1764527	None:intergenic	45.0%
	ACAGTGAATAGGTACCTAGG+TGG	+	chr1.1:1762935-1762954	MS.gene27251:intron	45.0%
	AGCATATGGAAGTAGAAGGG+AGG	+	chr1.1:1764866-1764885	MS.gene27251:CDS	45.0%
	AGGACAGTGAATAGGTACCT+AGG	+	chr1.1:1762932-1762951	MS.gene27251:intron	45.0%
	AGTCCTTTGTTAGAACTGCC+AGG	+	chr1.1:1762075-1762094	MS.gene27251:CDS	45.0%
	ATTAGGCTAGGTGGCATAGT+TGG	-	chr1.1:1764539-1764558	None:intergenic	45.0%
	CCAAAGCAATTTATCCCGAC+GGG	+	chr1.1:1762576-1762595	MS.gene27251:intron	45.0%
	CCCAAAGCAATTTATCCCGA+CGG	+	chr1.1:1762575-1762594	MS.gene27251:intron	45.0%
	CCGTCGGGATAAATTGCTTT+GGG	-	chr1.1:1762578-1762597	None:intergenic	45.0%
	CTCACAAGCTCCAATTGATC+TGG	-	chr1.1:1765090-1765109	None:intergenic	45.0%
	CTGAAGATCAAAAGCCCTTG+TGG	+	chr1.1:1764200-1764219	MS.gene27251:CDS	45.0%
	GAAGTCCATCTTGTGCTCTT+TGG	-	chr1.1:1764474-1764493	None:intergenic	45.0%
	GAGCAAACTGTCCATTTGGA+AGG	+	chr1.1:1762104-1762123	MS.gene27251:CDS	45.0%
	GTTGGGAATCGAGTCTGAAA+TGG	+	chr1.1:1764037-1764056	MS.gene27251:intron	45.0%
	TAGGAGAAAGGGCAGTGATT+AGG	-	chr1.1:1764556-1764575	None:intergenic	45.0%
	TTCCTTCCACGATTTAAGGG+AGG	+	chr1.1:1762630-1762649	MS.gene27251:intron	45.0%
	TTGATTTACACATCGTCCGC+CGG	+	chr1.1:1764163-1764182	MS.gene27251:CDS	45.0%
	TTGTGCACGATCTTGAGTAC+AGG	+	chr1.1:1764000-1764019	MS.gene27251:intron	45.0%
!	ATCATCCCACCTCTTGTTGT+TGG	+	chr1.1:1764500-1764519	MS.gene27251:CDS	45.0%
!	ATGGCTTGTCATGAGACTCA+TGG	+	chr1.1:1761803-1761822	MS.gene27251:CDS	45.0%
!	CATCATGAAAGCATGCCTCA+AGG	+	chr1.1:1764123-1764142	MS.gene27251:CDS	45.0%
!	GGAGTGATGTTGAGTTTTGC+AGG	+	chr1.1:1764236-1764255	MS.gene27251:CDS	45.0%
!	TGCCCCAAAGGCATATGTTA+AGG	-	chr1.1:1763186-1763205	None:intergenic	45.0%
!	TGGCAAAGATGGTGATGACA+TGG	+	chr1.1:1762955-1762974	MS.gene27251:intron	45.0%
	AAGGCTGACCTTGGTGAAGA+AGG	+	chr1.1:1762123-1762142	MS.gene27251:CDS	50.0%
	AATAAAAGGGACCTGGGTCG+CGG	-	chr1.1:1761857-1761876	None:intergenic	50.0%
	ACCAAGGTCAGCCTTCCAAA+TGG	-	chr1.1:1762118-1762137	None:intergenic	50.0%
	ACTGCCCTTTCTCCTATCAC+TGG	+	chr1.1:1764560-1764579	MS.gene27251:CDS	50.0%
	AGGTACCTAGGTGGCAAAGA+TGG	+	chr1.1:1762944-1762963	MS.gene27251:intron	50.0%
	CAACAGCTCTCATCCCACAA+GGG	-	chr1.1:1764217-1764236	None:intergenic	50.0%
	CACCTCCCTTAAATCGTGGA+AGG	-	chr1.1:1762635-1762654	None:intergenic	50.0%
	CATCACCATCTTTGCCACCT+AGG	-	chr1.1:1762952-1762971	None:intergenic	50.0%
	CCCGTCGGGATAAATTGCTT+TGG	-	chr1.1:1762579-1762598	None:intergenic	50.0%
	CCCTGTGACGCAAACAGTTT+CGG	-	chr1.1:1761763-1761782	None:intergenic	50.0%
	CCGAAACTGTTTGCGTCACA+GGG	+	chr1.1:1761760-1761779	MS.gene27251:CDS	50.0%
	CGAAACTGTTTGCGTCACAG+GGG	+	chr1.1:1761761-1761780	MS.gene27251:CDS	50.0%
	GAAAGGGCAGTGATTAGGCT+AGG	-	chr1.1:1764551-1764570	None:intergenic	50.0%
	GCAAACACTTTCCACGAGAG+AGG	+	chr1.1:1763879-1763898	MS.gene27251:intron	50.0%
	GCAAGGCCAATTTGTCCACT+TGG	+	chr1.1:1764810-1764829	MS.gene27251:CDS	50.0%
	TCCATTTGGAAGGCTGACCT+TGG	+	chr1.1:1762114-1762133	MS.gene27251:CDS	50.0%
	TGCAGGAGAGTGAAGATGAC+TGG	+	chr1.1:1764253-1764272	MS.gene27251:CDS	50.0%
	TGCCCTTAGGGCACAAGTTA+AGG	+	chr1.1:1763064-1763083	MS.gene27251:intron	50.0%
!	AGAGAGGATTTGCTCCCACT+GGG	+	chr1.1:1763895-1763914	MS.gene27251:intron	50.0%
!	AGGTGCCACAATACAAGGGA+GGG	+	chr1.1:1762650-1762669	MS.gene27251:intron	50.0%
!	AGTAACGTTTAGGGTTCCGG+CGG	-	chr1.1:1764182-1764201	None:intergenic	50.0%
!	GACAGTTTGCTCTTTGCACC+TGG	-	chr1.1:1762096-1762115	None:intergenic	50.0%
!	GAGAGTGAAGATGACTGGCT+GGG	+	chr1.1:1764258-1764277	MS.gene27251:CDS	50.0%
!	GCACCTGGCAGTTCTAACAA+AGG	-	chr1.1:1762081-1762100	None:intergenic	50.0%
!!	AAGGGAGGTGCCACAATACA+AGG	+	chr1.1:1762645-1762664	MS.gene27251:intron	50.0%
!!	AGGGAGGTGCCACAATACAA+GGG	+	chr1.1:1762646-1762665	MS.gene27251:intron	50.0%
!!	TTTTGAGTCCAAGGACCCTG+AGG	+	chr1.1:1762206-1762225	MS.gene27251:CDS	50.0%
!!!	TACGGACAGTTTTGGCCTTG+AGG	-	chr1.1:1764141-1764160	None:intergenic	50.0%
	ACTCATGGAGCGCGGTTACA+TGG	+	chr1.1:1761818-1761837	MS.gene27251:CDS	55.0%
	AGGGCAGTGATTAGGCTAGG+TGG	-	chr1.1:1764548-1764567	None:intergenic	55.0%
	CACTTGCGGAGCAAGAAGCA+TGG	+	chr1.1:1764441-1764460	MS.gene27251:CDS	55.0%
	CCAACAGCTCTCATCCCACA+AGG	-	chr1.1:1764218-1764237	None:intergenic	55.0%
	CCTTGTGGGATGAGAGCTGT+TGG	+	chr1.1:1764215-1764234	MS.gene27251:CDS	55.0%
	GACGCAAACAGTTTCGGCCA+TGG	-	chr1.1:1761757-1761776	None:intergenic	55.0%
	GACTGCCCTCCCTTGTATTG+TGG	-	chr1.1:1762658-1762677	None:intergenic	55.0%
	GCCGAAACTGTTTGCGTCAC+AGG	+	chr1.1:1761759-1761778	MS.gene27251:CDS	55.0%
	GGAGAGTGAAGATGACTGGC+TGG	+	chr1.1:1764257-1764276	MS.gene27251:CDS	55.0%
	GTCATGAGACTCATGGAGCG+CGG	+	chr1.1:1761810-1761829	MS.gene27251:CDS	55.0%
	GTGGCACCTCCCTTAAATCG+TGG	-	chr1.1:1762639-1762658	None:intergenic	55.0%
	TATGGGTACCTCAGGGTCCT+TGG	-	chr1.1:1762217-1762236	None:intergenic	55.0%
	TGGGAGCAAATCCTCTCTCG+TGG	-	chr1.1:1763893-1763912	None:intergenic	55.0%
	TGTTTGCGTCACAGGGGCTT+CGG	+	chr1.1:1761767-1761786	MS.gene27251:CDS	55.0%
!	ACAGGGGCTTCGGGTTTCAT+CGG	+	chr1.1:1761777-1761796	MS.gene27251:CDS	55.0%
!	CTTCGGGTTTCATCGGGTCA+TGG	+	chr1.1:1761784-1761803	MS.gene27251:CDS	55.0%
!	GAGAGAGGATTTGCTCCCAC+TGG	+	chr1.1:1763894-1763913	MS.gene27251:intron	55.0%
!	GAGGTGCCACAATACAAGGG+AGG	+	chr1.1:1762649-1762668	MS.gene27251:intron	55.0%
	GTTTGCGTCACAGGGGCTTC+GGG	+	chr1.1:1761768-1761787	MS.gene27251:CDS	60.0%
!	CAGGGGCTTCGGGTTTCATC+GGG	+	chr1.1:1761778-1761797	MS.gene27251:CDS	60.0%
	CGAGCAACAGTCCGCGACCC+AGG	+	chr1.1:1761843-1761862	MS.gene27251:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	1761747	1765260	1761747	ID=MS.gene27251
chr1.1	mRNA	1761747	1765260	1761747	ID=MS.gene27251.t1;Parent=MS.gene27251
chr1.1	exon	1761747	1761864	1761747	ID=MS.gene27251.t1.exon1;Parent=MS.gene27251.t1
chr1.1	CDS	1761747	1761864	1761747	ID=cds.MS.gene27251.t1;Parent=MS.gene27251.t1
chr1.1	exon	1762058	1762227	1762058	ID=MS.gene27251.t1.exon2;Parent=MS.gene27251.t1
chr1.1	CDS	1762058	1762227	1762058	ID=cds.MS.gene27251.t1;Parent=MS.gene27251.t1
chr1.1	exon	1764085	1764279	1764085	ID=MS.gene27251.t1.exon3;Parent=MS.gene27251.t1
chr1.1	CDS	1764085	1764279	1764085	ID=cds.MS.gene27251.t1;Parent=MS.gene27251.t1
chr1.1	exon	1764422	1764581	1764422	ID=MS.gene27251.t1.exon4;Parent=MS.gene27251.t1
chr1.1	CDS	1764422	1764581	1764422	ID=cds.MS.gene27251.t1;Parent=MS.gene27251.t1
chr1.1	exon	1764782	1764974	1764782	ID=MS.gene27251.t1.exon5;Parent=MS.gene27251.t1
chr1.1	CDS	1764782	1764974	1764782	ID=cds.MS.gene27251.t1;Parent=MS.gene27251.t1
chr1.1	exon	1765074	1765260	1765074	ID=MS.gene27251.t1.exon6;Parent=MS.gene27251.t1
chr1.1	CDS	1765074	1765260	1765074	ID=cds.MS.gene27251.t1;Parent=MS.gene27251.t1

Gene Sequence

>MS.gene27251

ATGGGTTCCATGGCCGAAACTGTTTGCGTCACAGGGGCTTCGGGTTTCATCGGGTCATGGCTTGTCATGAGACTCATGGAGCGCGGTTACATGGTTCGAGCAACAGTCCGCGACCCAGGTCCCTTTTATTTTTATGTTGCAACATTTTTCATATCATATATAAATCAATTCATATTTTAACACCAACAATTTTTTTAATCAAAATTCAGCTGTTTGATACTAACAAAATAATGTAAGAGTGTGTTTTGACTAGAGATTTCAAAACTCAAGAAAATTGATGGGTTTATATATACACACATTTTGGAATGCAGAAAACTTGAAGAAGGTGAGTCCTTTGTTAGAACTGCCAGGTGCAAAGAGCAAACTGTCCATTTGGAAGGCTGACCTTGGTGAAGAAGGTAGTTTTGATGAAGCAATTAAAGGGTGTACTGGAGTTTTTCATGTGGCTACACCTATGGATTTTGAGTCCAAGGACCCTGAGGTACCCATACTTTAATTTTATATGCATCTATCATCTCATATATGAATGGCACTAGTCTCTCTCTCTCTCTCTCTCTCCCTCGCAAAATATTGAATTTCCAAAGAACATTGCTTCATAGTGATCTAATAATTTCTCAACCTAGTATAACATCCTTTGGGATGTTTATCATTTTCCTTTTTTGTAACCATCCCATTACTACATTGATAATAACCATTAAATGTATGAACCGTTTCCATTTTAGTAGTTACTCACCATTAATCCAATATATATAATGGTATTAAAACATTGATGAAGTGATATTTCAAATATGAATGAACATATTTTGAGCATACAGTATTGCTATATCTCCCAAAGCAATTTATCCCGACGGGATCACGACTAATTTACAAGCATATAATTTTGTTCCTTCCACGATTTAAGGGAGGTGCCACAATACAAGGGAGGGCAGTCTTGTAATTTAGAAAAAATACATTCAGTAGAAATCAAGGGTCGTGAAAATTTCGCTAGCTTATCCATCAGGATAACTAGCATTATTCTTGAGCATAATGTAATTTATTAGGGGTGATGGTTTGTTAGATTCTCTATGGTGGAGAGTGTCTTAGTTTTATACACACAGTTTATGTAAAACTTCAAATGTTTAATTGAGAGGACATAATATGTGACTCCAACTTTGTTCTATTTGTGTTCAACATCATCTAGTGATAAGGACAGTGAATAGGTACCTAGGTGGCAAAGATGGTGATGACATGGATGTCACAACTATTTTTTTAAAGGGTTAATGACAATGATTTGGGTTGCTATAGATATCTAAGTTTTTTTTGGGTCTTGTTATCTTGTGCCCTTAGGGCACAAGTTAAGGAATCCAAAATTAGAAATTTTTTATGGAAAAAAGTAAAACTTTGACTTTAAAAAGTTGAATGCACAACTTTTAAGACAACCATTTCTATTTTAAATTCCTTAACATATGCCTTTGGGGCACATGTTAACATTTTCCAAGTTTTAAATATCTACGTAAATGAGATTGAGATGGTATTGATCACATTTGACCATAATTTTCAGTTATAGTTTTTTGACAAAAGCTTTATGTAGTATTAACGATTGTGACACGACGAAAATGATAAAAGTGAATTAAAATTTTGGTGATAATGGTGGTTGAGAAGTAAAACAAATGGTTGATTGGTGGTTAATACTTCAAAAAGACTTTTTTAATAGCCTCTTCATTATTCAAGGGGATGATAAAAGTGAATTAAAATTTTGGTGATAATGGTGGTTGAGAAGTAAAACAAATGGTTGATTGGTGGTTAATACTTCAAAAAGACTTTTTTAATAGCCTCTTCATTATTCAAGGGGATTGTGAGTTGAAAAAAAAAAATAGTCACCACCATATACTAAAGTTTATAAAAAATTAATCAATAAAACTCCAAAATTACAAATCAAACTATATAATTCAAATAAATTGTAACATAGAAATATATGATCGCAAACCTAACAATCTAATCATTTTTTTTTTTGGACTCAGTCATAAATATAACCTGAACACTGATCATCTCTTGTGAAAAATTTATTGGAGTGTCTCACGAAAATTCCTTTTACTGATTTTTTATTATATATTTATCTGCTGCAAAAAGAAATGTGTGGCTATGATTTCACTGCAAACACTTTCCACGAGAGAGGATTTGCTCCCACTGGGTAGCTATAAATATATTTAAGAAATTTGTGCACGATCTTAAGTAGTGGTGTATAACAATATGGTTAAAAAAATATTTAAGAAATTGTGCACGATCTTGAGTACAGGTGTATAACAATATGGTTGGGAATCGAGTCTGAAATGGTTTGAAATTTTGATTTGAACTGCAGAATGAAATGATCAAGCCTACCATAAAAGGAGTGCTAGACATCATGAAAGCATGCCTCAAGGCCAAAACTGTCCGTAGATTGATTTACACATCGTCCGCCGGAACCCTAAACGTTACTGAAGATCAAAAGCCCTTGTGGGATGAGAGCTGTTGGAGTGATGTTGAGTTTTGCAGGAGAGTGAAGATGACTGGCTGGGTAAGTTAGAAATTTATTTTCATAAATACATTCTCTTCACTTGAATTTTAACCTATAAGAGTTTTATAATTTAATTACGTCCTAATCACTTTAAATTAAATTAACAATTAATGCATATCTTTATTTTATATTTTTTGTTTAGATGTATTTTGTTTCTAAGACACTTGCGGAGCAAGAAGCATGGAAATTTGCCAAAGAGCACAAGATGGACTTCATCACAATCATCCCACCTCTTGTTGTTGGTCCTTTTCTTATTCCAACTATGCCACCTAGCCTAATCACTGCCCTTTCTCCTATCACTGGTACATTTATAAATATAATTTTCTAAATTTAGAAAACATATTGCAATAATAACATGCTTAAATTCCTTAAAAAATTGGAAATGATTCTCATATAATTTAAACTTAATTCGTCTCTTAATATTATAATATCAAACTCTTACCACCGTCTACTACTAAGTAACAATTGATCGAAGATAACATGGTTTTGCACAAATTTGCAGGAAATGAGGCTCATTATTCGATTATAAAGCAAGGCCAATTTGTCCACTTGGATGATCTTTGTGAAGCACACATATTCTTGTTTGAGCATATGGAAGTAGAAGGGAGGTATCTATGTAGTGCATATGAAGCTAATATTCATGACATTGCAAAATTAATTAATACAAAATACCCAGAGTACAATATCCCCACAAAGTAAGTACTTAATGCTACTTCAAGTATTAAAGTTAAAATGCACACTTAAATAATAGATGAAACTTTATGCTTGTCTAATTGTTTAAATTGGATTTTTAGGTTCAAGAATATTCCAGATCAATTGGAGCTTGTGAGATTTTCATCAAAGAAGATCAAAGACTTGGGATTTGAATTCAAATACAGCTTGGAGGATATGTACACTGAAGCAATTGATACATGCAGAGAAAAAGGGCTTCTTCCTGAATTTGTTAAAGCACCAATAAGTAATGGTGTCACACATAAATAA

Protein sequence

>MS.gene27251.t1

MGSMAETVCVTGASGFIGSWLVMRLMERGYMVRATVRDPENLKKVSPLLELPGAKSKLSIWKADLGEEGSFDEAIKGCTGVFHVATPMDFESKDPENEMIKPTIKGVLDIMKACLKAKTVRRLIYTSSAGTLNVTEDQKPLWDESCWSDVEFCRRVKMTGWMYFVSKTLAEQEAWKFAKEHKMDFITIIPPLVVGPFLIPTMPPSLITALSPITGNEAHYSIIKQGQFVHLDDLCEAHIFLFEHMEVEGRYLCSAYEANIHDIAKLINTKYPEYNIPTKFKNIPDQLELVRFSSKKIKDLGFEFKYSLEDMYTEAIDTCREKGLLPEFVKAPISNGVTHK