Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059529.t1 | XP_013456841.1 | 95.9 | 438 | 17 | 1 | 1 | 437 | 1 | 438 | 4.00E-191 | 677.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059529.t1 | Q8RWZ6 | 59.2 | 461 | 79 | 7 | 1 | 423 | 1 | 390 | 8.5e-126 | 451.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059529.t1 | Q8GV76 | 95.9 | 438 | 17 | 1 | 1 | 437 | 1 | 438 | 2.9e-191 | 677.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049907 | MS.gene059529 | 0.818727 | 1.69E-52 | -1.69E-46 |
| MS.gene053813 | MS.gene059529 | 0.816363 | 5.75E-52 | -1.69E-46 |
| MS.gene05492 | MS.gene059529 | 0.809846 | 1.55E-50 | -1.69E-46 |
| MS.gene056931 | MS.gene059529 | 0.830962 | 2.19E-55 | -1.69E-46 |
| MS.gene056933 | MS.gene059529 | 0.832478 | 9.24E-56 | -1.69E-46 |
| MS.gene056935 | MS.gene059529 | 0.821369 | 4.19E-53 | -1.69E-46 |
| MS.gene059529 | MS.gene059530 | 0.922529 | 8.73E-89 | -1.69E-46 |
| MS.gene059529 | MS.gene060356 | 0.848334 | 6.55E-60 | -1.69E-46 |
| MS.gene059529 | MS.gene060559 | 0.8084 | 3.16E-50 | -1.69E-46 |
| MS.gene059529 | MS.gene060837 | 0.808937 | 2.42E-50 | -1.69E-46 |
| MS.gene059529 | MS.gene061801 | 0.80816 | 3.55E-50 | -1.69E-46 |
| MS.gene059529 | MS.gene065204 | 0.832473 | 9.27E-56 | -1.69E-46 |
| MS.gene059529 | MS.gene069435 | 0.805952 | 1.04E-49 | -1.69E-46 |
| MS.gene059529 | MS.gene073007 | 0.842845 | 2.02E-58 | -1.69E-46 |
| MS.gene059529 | MS.gene21413 | 0.80031 | 1.53E-48 | -1.69E-46 |
| MS.gene059529 | MS.gene22224 | 0.805803 | 1.12E-49 | -1.69E-46 |
| MS.gene059529 | MS.gene22673 | 0.813191 | 2.90E-51 | -1.69E-46 |
| MS.gene059529 | MS.gene24248 | 0.800329 | 1.52E-48 | -1.69E-46 |
| MS.gene059529 | MS.gene25858 | 0.809944 | 1.47E-50 | -1.69E-46 |
| MS.gene059529 | MS.gene26989 | 0.804273 | 2.34E-49 | -1.69E-46 |
| MS.gene059529 | MS.gene27251 | 0.800741 | 1.25E-48 | -1.69E-46 |
| MS.gene059529 | MS.gene29785 | 0.804897 | 1.73E-49 | -1.69E-46 |
| MS.gene059529 | MS.gene33036 | 0.823876 | 1.09E-53 | -1.69E-46 |
| MS.gene059529 | MS.gene35043 | 0.830861 | 2.31E-55 | -1.69E-46 |
| MS.gene059529 | MS.gene44994 | 0.805255 | 1.46E-49 | -1.69E-46 |
| MS.gene059529 | MS.gene46507 | 0.817116 | 3.90E-52 | -1.69E-46 |
| MS.gene059529 | MS.gene49928 | 0.836986 | 6.79E-57 | -1.69E-46 |
| MS.gene059529 | MS.gene57316 | 0.801782 | 7.65E-49 | -1.69E-46 |
| MS.gene059529 | MS.gene61160 | 0.810007 | 1.43E-50 | -1.69E-46 |
| MS.gene059529 | MS.gene63451 | 0.820282 | 7.45E-53 | -1.69E-46 |
| MS.gene059529 | MS.gene70393 | 0.825877 | 3.69E-54 | -1.69E-46 |
| MS.gene059529 | MS.gene84849 | 0.813885 | 2.04E-51 | -1.69E-46 |
| MS.gene059529 | MS.gene85315 | 0.821298 | 4.35E-53 | -1.69E-46 |
| MS.gene059529 | MS.gene85662 | 0.851823 | 6.91E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059529.t1 | MTR_4g084870 | 97.260 | 438 | 11 | 1 | 1 | 437 | 1 | 438 | 0.0 | 887 |
| MS.gene059529.t1 | MTR_1g030890 | 77.854 | 438 | 55 | 11 | 1 | 436 | 1 | 398 | 0.0 | 631 |
| MS.gene059529.t1 | MTR_1g030890 | 77.854 | 438 | 55 | 11 | 1 | 436 | 1 | 398 | 0.0 | 630 |
| MS.gene059529.t1 | MTR_6g069510 | 61.149 | 435 | 110 | 8 | 1 | 422 | 1 | 389 | 9.77e-174 | 500 |
| MS.gene059529.t1 | MTR_4g127100 | 56.410 | 429 | 136 | 9 | 1 | 407 | 1 | 400 | 3.48e-154 | 448 |
| MS.gene059529.t1 | MTR_7g089360 | 56.481 | 432 | 113 | 8 | 1 | 416 | 1 | 373 | 3.18e-153 | 447 |
| MS.gene059529.t1 | MTR_4g127100 | 56.410 | 429 | 136 | 9 | 1 | 407 | 1 | 400 | 4.24e-153 | 448 |
| MS.gene059529.t1 | MTR_7g089360 | 56.481 | 432 | 113 | 8 | 1 | 416 | 1 | 373 | 5.75e-153 | 446 |
| MS.gene059529.t1 | MTR_8g107360 | 58.747 | 383 | 91 | 9 | 1 | 378 | 1 | 321 | 4.30e-144 | 421 |
| MS.gene059529.t1 | MTR_4g127090 | 56.316 | 380 | 137 | 9 | 1 | 355 | 1 | 376 | 2.50e-134 | 398 |
| MS.gene059529.t1 | MTR_1g029190 | 54.515 | 299 | 106 | 7 | 1 | 288 | 1 | 280 | 1.12e-97 | 303 |
| MS.gene059529.t1 | MTR_2g038470 | 47.410 | 251 | 110 | 3 | 1 | 251 | 1 | 229 | 1.88e-73 | 239 |
| MS.gene059529.t1 | MTR_7g009370 | 54.430 | 158 | 72 | 0 | 1 | 158 | 1 | 158 | 1.73e-52 | 180 |
| MS.gene059529.t1 | MTR_6g011400 | 54.430 | 158 | 71 | 1 | 1 | 157 | 1 | 158 | 1.12e-50 | 175 |
| MS.gene059529.t1 | MTR_7g079720 | 51.613 | 155 | 75 | 0 | 1 | 155 | 1 | 155 | 2.40e-49 | 172 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059529.t1 | AT2G01420 | 75.234 | 428 | 63 | 9 | 1 | 423 | 1 | 390 | 0.0 | 622 |
| MS.gene059529.t1 | AT1G70940 | 75.174 | 431 | 70 | 11 | 1 | 423 | 1 | 402 | 0.0 | 622 |
| MS.gene059529.t1 | AT2G01420 | 74.424 | 434 | 68 | 9 | 1 | 429 | 1 | 396 | 0.0 | 622 |
| MS.gene059529.t1 | AT2G01420 | 75.234 | 428 | 63 | 9 | 1 | 423 | 1 | 390 | 0.0 | 621 |
| MS.gene059529.t1 | AT1G23080 | 72.311 | 437 | 73 | 11 | 1 | 429 | 1 | 397 | 0.0 | 605 |
| MS.gene059529.t1 | AT1G23080 | 72.622 | 431 | 71 | 10 | 1 | 423 | 1 | 392 | 0.0 | 603 |
| MS.gene059529.t1 | AT1G23080 | 72.622 | 431 | 71 | 10 | 1 | 423 | 1 | 392 | 0.0 | 603 |
| MS.gene059529.t1 | AT1G23080 | 72.622 | 431 | 71 | 10 | 1 | 423 | 1 | 392 | 0.0 | 602 |
| MS.gene059529.t1 | AT1G73590 | 57.241 | 435 | 146 | 8 | 1 | 421 | 1 | 409 | 9.60e-160 | 465 |
| MS.gene059529.t1 | AT5G57090 | 56.582 | 433 | 142 | 8 | 1 | 405 | 1 | 415 | 4.89e-152 | 446 |
| MS.gene059529.t1 | AT1G77110 | 43.672 | 403 | 184 | 9 | 1 | 372 | 1 | 391 | 1.07e-100 | 311 |
| MS.gene059529.t1 | AT1G77110 | 43.672 | 403 | 184 | 9 | 1 | 372 | 1 | 391 | 2.07e-100 | 311 |
| MS.gene059529.t1 | AT5G57090 | 51.051 | 333 | 117 | 8 | 101 | 405 | 1 | 315 | 4.95e-89 | 281 |
| MS.gene059529.t1 | AT5G16530 | 52.866 | 157 | 74 | 0 | 1 | 157 | 1 | 157 | 5.89e-51 | 175 |
| MS.gene059529.t1 | AT5G16530 | 52.866 | 157 | 74 | 0 | 1 | 157 | 1 | 157 | 2.01e-50 | 174 |
| MS.gene059529.t1 | AT5G15100 | 48.023 | 177 | 82 | 2 | 1 | 172 | 1 | 172 | 5.96e-46 | 163 |
Find 116 sgRNAs with CRISPR-Local
Find 125 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTCTATTGTTTCCTTTA+AGG | 0.025864 | 4.4:+27794354 | MS.gene059529:CDS |
| GCTCCGAGACATTCTAATTT+TGG | 0.163099 | 4.4:+27794619 | MS.gene059529:CDS |
| TGCTCTTTGCACTTACAATT+TGG | 0.180770 | 4.4:+27794092 | MS.gene059529:CDS |
| ACCCTCGCGGTGTCGGATTC+CGG | 0.228563 | 4.4:-27794555 | None:intergenic |
| ACAATAGAAGCAGCAGTTTC+AGG | 0.257344 | 4.4:-27794343 | None:intergenic |
| ATTACCCAACACTTTGGTTA+TGG | 0.281307 | 4.4:+27794174 | MS.gene059529:CDS |
| CCAAACCCGGAATTCTCTTC+CGG | 0.282860 | 4.4:+27794805 | MS.gene059529:CDS |
| TCCGATGTTGTTTCGCTTGA+TGG | 0.307846 | 4.4:+27794382 | MS.gene059529:CDS |
| TGGAGTCGGCCCACGGGAAT+TGG | 0.308120 | 4.4:-27794668 | None:intergenic |
| GCTGTGAACTCTCCGAAATT+TGG | 0.309850 | 4.4:+27794742 | MS.gene059529:CDS |
| AACATCGGAATCAACCTTAA+AGG | 0.310000 | 4.4:-27794368 | None:intergenic |
| CTCAACCCGGAAGAGAATTC+CGG | 0.312908 | 4.4:-27794811 | None:intergenic |
| TTACCCAACACTTTGGTTAT+GGG | 0.317734 | 4.4:+27794175 | MS.gene059529:CDS |
| TCTTCGAAGTTTGACGGCCT+TGG | 0.319467 | 4.4:-27794688 | None:intergenic |
| CCCTCGCGGTGTCGGATTCC+GGG | 0.322603 | 4.4:-27794554 | None:intergenic |
| TGAATATTCCGGTACCCTTA+TGG | 0.333095 | 4.4:+27794225 | MS.gene059529:CDS |
| GCCTGCGCCGCTGTTGCAGC+TGG | 0.339883 | 4.4:-27794721 | None:intergenic |
| TGGACTAATTTCACAGCTAA+TGG | 0.361197 | 4.4:+27794112 | MS.gene059529:CDS |
| CCGGAAGAGAATTCCGGGTT+TGG | 0.361318 | 4.4:-27794805 | None:intergenic |
| TCAGAAAATCAAACGCTTCC+CGG | 0.368913 | 4.4:+27794464 | MS.gene059529:CDS |
| GCCGCTGTTGCAGCTGGCGC+AGG | 0.369809 | 4.4:-27794715 | None:intergenic |
| TGCTATGAACCTATAGTTCA+TGG | 0.375035 | 4.4:-27794044 | None:intergenic |
| CAAACCCGGAATTCTCTTCC+GGG | 0.382809 | 4.4:+27794806 | MS.gene059529:CDS |
| GACTTGAAGCCCGCTGGCTT+CGG | 0.385833 | 4.4:-27795004 | None:intergenic |
| AACCTATAGTTCATGGCATA+AGG | 0.386855 | 4.4:-27794037 | None:intergenic |
| TCCTGCGCCAGCTGCAACAG+CGG | 0.394025 | 4.4:+27794714 | MS.gene059529:CDS |
| GCCAGCTGCAACAGCGGCGC+AGG | 0.397031 | 4.4:+27794720 | MS.gene059529:CDS |
| AGCCCGCTGGCTTCGGATAC+CGG | 0.401191 | 4.4:-27794997 | None:intergenic |
| AATCTCTTTAGCACCTTGTT+CGG | 0.402405 | 4.4:-27795076 | None:intergenic |
| TCAACCCGGAAGAGAATTCC+GGG | 0.410364 | 4.4:-27794810 | None:intergenic |
| GCCGGATAAAAGCCAAATTT+CGG | 0.412131 | 4.4:-27794754 | None:intergenic |
| TGGTTCTTCAGTGTATCATT+TGG | 0.415072 | 4.4:+27794257 | MS.gene059529:CDS |
| TGCTAAAGAGATTAGAATGT+TGG | 0.418993 | 4.4:+27795086 | MS.gene059529:CDS |
| GGTAGTGGTGCTACTGATTA+CGG | 0.422285 | 4.4:+27795030 | MS.gene059529:CDS |
| GCTATGTATGGTGAATATTC+CGG | 0.424658 | 4.4:+27794214 | MS.gene059529:CDS |
| TTGGTAACAGTAACACCATT+TGG | 0.429685 | 4.4:-27795126 | None:intergenic |
| TTTATAGTTTGAGTTCTTCC+CGG | 0.431327 | 4.4:+27794536 | MS.gene059529:CDS |
| TCCGGTGAGATTCGAGGGCC+GGG | 0.432350 | 4.4:-27794506 | None:intergenic |
| CGCCGGTATCCGAAGCCAGC+GGG | 0.433380 | 4.4:+27794995 | MS.gene059529:CDS |
| ACCCTTATGCCATGAACTAT+AGG | 0.435554 | 4.4:+27794035 | MS.gene059529:CDS |
| GAGAATTCCGGGTTTGGAGC+AGG | 0.437222 | 4.4:-27794799 | None:intergenic |
| GAACCACAACTTGAACCATA+AGG | 0.450783 | 4.4:-27794240 | None:intergenic |
| TACCCTTATGGTTCAAGTTG+TGG | 0.453299 | 4.4:+27794237 | MS.gene059529:CDS |
| TACGGATTTCTACTCTATGA+TGG | 0.460158 | 4.4:+27794591 | MS.gene059529:CDS |
| CTCCGGTGAGATTCGAGGGC+CGG | 0.462423 | 4.4:-27794507 | None:intergenic |
| AAATTAGACCCTCGCGGTGT+CGG | 0.464343 | 4.4:-27794562 | None:intergenic |
| GGATTCTCTTCGAAGTTTGA+CGG | 0.468324 | 4.4:-27794694 | None:intergenic |
| CCACTTCTCATAGCTATGTA+TGG | 0.471301 | 4.4:+27794202 | MS.gene059529:CDS |
| ATTCCCGTGGGCCGACTCCA+AGG | 0.485087 | 4.4:+27794671 | MS.gene059529:CDS |
| AAAATCATGGCTACGTAAAG+TGG | 0.485356 | 4.4:-27793893 | None:intergenic |
| CCATTGTTAGTAACTGTTTG+TGG | 0.486279 | 4.4:-27794904 | None:intergenic |
| ACCTATAGTTCATGGCATAA+GGG | 0.489456 | 4.4:-27794036 | None:intergenic |
| GCCAGCGGGCTTCAAGTCTT+CGG | 0.489637 | 4.4:+27795009 | MS.gene059529:CDS |
| AACTGTGTAGAGATCGTGCC+AGG | 0.494115 | 4.4:-27793855 | None:intergenic |
| TACGGAGCGTCTGATCAATC+TGG | 0.494664 | 4.4:+27795048 | MS.gene059529:CDS |
| TTCTCACCGGACCAATGCTC+CGG | 0.496572 | 4.4:+27793947 | MS.gene059529:CDS |
| GAGTCATCATAAAAGATCTC+CGG | 0.496982 | 4.4:-27794483 | None:intergenic |
| ATTCCCATAACCAAAGTGTT+GGG | 0.498180 | 4.4:-27794178 | None:intergenic |
| AAGTTTGACGGCCTTGGAGT+CGG | 0.501514 | 4.4:-27794682 | None:intergenic |
| GATGCTGAGGTTGGTGATGA+TGG | 0.502427 | 4.4:+27794424 | MS.gene059529:CDS |
| CTCTGCTCCGGTGAGATTCG+AGG | 0.503401 | 4.4:-27794512 | None:intergenic |
| AACGGTTTATGCCGGAGCAT+TGG | 0.504476 | 4.4:-27793958 | None:intergenic |
| GGGCTTCAAGTCTTCGGTAG+TGG | 0.505488 | 4.4:+27795015 | MS.gene059529:CDS |
| ACGGCCTTGGAGTCGGCCCA+CGG | 0.507146 | 4.4:-27794675 | None:intergenic |
| CTGCGAAAATCGCGACGAAA+CGG | 0.508205 | 4.4:-27793976 | None:intergenic |
| AGCTTACCCTGCTCCAAACC+CGG | 0.511734 | 4.4:+27794792 | MS.gene059529:CDS |
| CCTTCATCAAAATGATAACC+TGG | 0.515680 | 4.4:+27793837 | None:intergenic |
| TTCAACATTACCCAACACTT+TGG | 0.515765 | 4.4:+27794168 | MS.gene059529:CDS |
| AACCACAACTTGAACCATAA+GGG | 0.517091 | 4.4:-27794239 | None:intergenic |
| TCCGTTCAGTCCAATTCCCG+TGG | 0.520147 | 4.4:+27794658 | MS.gene059529:CDS |
| GTGGAGCTCTAGTGCCTCGC+CGG | 0.529135 | 4.4:+27794978 | MS.gene059529:CDS |
| TTTATGCCGGAGCATTGGTC+CGG | 0.532539 | 4.4:-27793953 | None:intergenic |
| TCTGATGACCATCCACCAAA+TGG | 0.532819 | 4.4:+27795111 | MS.gene059529:CDS |
| CAATCTGGACGCTCCGAACA+AGG | 0.534737 | 4.4:+27795063 | MS.gene059529:CDS |
| CCACCACCGTACGGAGCCAT+AGG | 0.539416 | 4.4:-27793918 | None:intergenic |
| CGGCCTTGGAGTCGGCCCAC+GGG | 0.539777 | 4.4:-27794674 | None:intergenic |
| CATGGCATAAGGGTTATTTG+TGG | 0.541794 | 4.4:-27794026 | None:intergenic |
| CTTGAGACAGATGCTGAGGT+TGG | 0.542841 | 4.4:+27794415 | MS.gene059529:CDS |
| CCTATGGCTCCGTACGGTGG+TGG | 0.543458 | 4.4:+27793918 | MS.gene059529:CDS |
| CGCGACGAAACGGTTTATGC+CGG | 0.546741 | 4.4:-27793966 | None:intergenic |
| TGTGAAGCTCTTTAGCATCA+TGG | 0.550496 | 4.4:-27794948 | None:intergenic |
| CCCCGGCCCTCGAATCTCAC+CGG | 0.552582 | 4.4:+27794505 | MS.gene059529:CDS |
| AATTCCCATAACCAAAGTGT+TGG | 0.554129 | 4.4:-27794179 | None:intergenic |
| AACTTGAACCATAAGGGTAC+CGG | 0.554608 | 4.4:-27794233 | None:intergenic |
| ACCGAAGACTTGAAGCCCGC+TGG | 0.555211 | 4.4:-27795010 | None:intergenic |
| AGAATTCCGGGTTTGGAGCA+GGG | 0.556853 | 4.4:-27794798 | None:intergenic |
| TTTGGAGCAGGGTAAGCTGC+CGG | 0.561589 | 4.4:-27794787 | None:intergenic |
| GCACCAAAATTAGAATGTCT+CGG | 0.565935 | 4.4:-27794622 | None:intergenic |
| ACGGATTTCTACTCTATGAT+GGG | 0.566049 | 4.4:+27794592 | MS.gene059529:CDS |
| CAAACTATAAATCTCTGCTC+CGG | 0.566086 | 4.4:-27794524 | None:intergenic |
| ATGAAATGAAAGGATAGAAG+AGG | 0.566566 | 4.4:-27794001 | None:intergenic |
| GTAACAGTAACACCATTTGG+TGG | 0.569554 | 4.4:-27795123 | None:intergenic |
| CCATACATAGCTATGAGAAG+TGG | 0.569788 | 4.4:-27794202 | None:intergenic |
| AGTCATCATAAAAGATCTCC+GGG | 0.571269 | 4.4:-27794482 | None:intergenic |
| TTATCCGGCACAAAATGTGC+CGG | 0.572329 | 4.4:+27794768 | MS.gene059529:CDS |
| TCTGCTCCGGTGAGATTCGA+GGG | 0.574304 | 4.4:-27794511 | None:intergenic |
| CCGGTGAGATTCGAGGGCCG+GGG | 0.575912 | 4.4:-27794505 | None:intergenic |
| CAGCTAATGGTAGCTTAGAG+TGG | 0.576924 | 4.4:+27794125 | MS.gene059529:CDS |
| ATTTGTGGAGATGAAATGAA+AGG | 0.580978 | 4.4:-27794011 | None:intergenic |
| CAGTAACACCATTTGGTGGA+TGG | 0.582761 | 4.4:-27795119 | None:intergenic |
| CCCGGAATCCGACACCGCGA+GGG | 0.584878 | 4.4:+27794554 | MS.gene059529:CDS |
| GGTGTTACTGTTACCAACAA+AGG | 0.585850 | 4.4:+27795132 | MS.gene059529:CDS |
| CCACAAACAGTTACTAACAA+TGG | 0.587706 | 4.4:+27794904 | MS.gene059529:CDS |
| TCGCCGGTATCCGAAGCCAG+CGG | 0.592686 | 4.4:+27794994 | MS.gene059529:CDS |
| CCCACGGGAATTGGACTGAA+CGG | 0.605853 | 4.4:-27794659 | None:intergenic |
| CCGTTCAGTCCAATTCCCGT+GGG | 0.607841 | 4.4:+27794659 | MS.gene059529:CDS |
| TCCATCAAGCGAAACAACAT+CGG | 0.608873 | 4.4:-27794383 | None:intergenic |
| TCCCGGAATCCGACACCGCG+AGG | 0.609770 | 4.4:+27794553 | MS.gene059529:CDS |
| AAGAGCTTCACATGTTTGTG+TGG | 0.615015 | 4.4:+27794959 | MS.gene059529:CDS |
| ACTTACACTCTTGCTCAACC+CGG | 0.624193 | 4.4:-27794824 | None:intergenic |
| GGAGCCATAGGCTAAAATCA+TGG | 0.627873 | 4.4:-27793906 | None:intergenic |
| TAGCCTATGGCTCCGTACGG+TGG | 0.633812 | 4.4:+27793915 | MS.gene059529:CDS |
| GTGGTGGAAAATATTCTCAC+CGG | 0.633995 | 4.4:+27793934 | MS.gene059529:CDS |
| GCTGGCTTCGGATACCGGCG+AGG | 0.674655 | 4.4:-27794992 | None:intergenic |
| TACGTAAAGTGGAACTACTG+CGG | 0.698065 | 4.4:-27793882 | None:intergenic |
| TGATTAAAATTAGACCCTCG+CGG | 0.741283 | 4.4:-27794568 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGGGTCTAATTTTAATCATA+CGG | + | chr4.4:27794573-27794592 | MS.gene059529:CDS | 25.0% |
| ATGAAATGAAAGGATAGAAG+AGG | - | chr4.4:27794004-27794023 | None:intergenic | 30.0% | |
| ATTTGTGGAGATGAAATGAA+AGG | - | chr4.4:27794014-27794033 | None:intergenic | 30.0% | |
| TGCTAAAGAGATTAGAATGT+TGG | + | chr4.4:27795086-27795105 | MS.gene059529:CDS | 30.0% | |
| TGCTTCTATTGTTTCCTTTA+AGG | + | chr4.4:27794354-27794373 | MS.gene059529:CDS | 30.0% | |
| ! | TTTATAGTTTGAGTTCTTCC+CGG | + | chr4.4:27794536-27794555 | MS.gene059529:CDS | 30.0% |
| !!! | TTGTTTTTGTTTGAGTATCG+TGG | + | chr4.4:27794292-27794311 | MS.gene059529:CDS | 30.0% |
| AAAATCATGGCTACGTAAAG+TGG | - | chr4.4:27793896-27793915 | None:intergenic | 35.0% | |
| AACATCGGAATCAACCTTAA+AGG | - | chr4.4:27794371-27794390 | None:intergenic | 35.0% | |
| AACCACAACTTGAACCATAA+GGG | - | chr4.4:27794242-27794261 | None:intergenic | 35.0% | |
| AACCTATAGTTCATGGCATA+AGG | - | chr4.4:27794040-27794059 | None:intergenic | 35.0% | |
| AATCTCTTTAGCACCTTGTT+CGG | - | chr4.4:27795079-27795098 | None:intergenic | 35.0% | |
| AATTCCCATAACCAAAGTGT+TGG | - | chr4.4:27794182-27794201 | None:intergenic | 35.0% | |
| ACCTATAGTTCATGGCATAA+GGG | - | chr4.4:27794039-27794058 | None:intergenic | 35.0% | |
| ACGGATTTCTACTCTATGAT+GGG | + | chr4.4:27794592-27794611 | MS.gene059529:CDS | 35.0% | |
| AGTCATCATAAAAGATCTCC+GGG | - | chr4.4:27794485-27794504 | None:intergenic | 35.0% | |
| ATTACCCAACACTTTGGTTA+TGG | + | chr4.4:27794174-27794193 | MS.gene059529:CDS | 35.0% | |
| CAAACTATAAATCTCTGCTC+CGG | - | chr4.4:27794527-27794546 | None:intergenic | 35.0% | |
| CCACAAACAGTTACTAACAA+TGG | + | chr4.4:27794904-27794923 | MS.gene059529:CDS | 35.0% | |
| CCATTGTTAGTAACTGTTTG+TGG | - | chr4.4:27794907-27794926 | None:intergenic | 35.0% | |
| GAGTCATCATAAAAGATCTC+CGG | - | chr4.4:27794486-27794505 | None:intergenic | 35.0% | |
| GCACCAAAATTAGAATGTCT+CGG | - | chr4.4:27794625-27794644 | None:intergenic | 35.0% | |
| GCTATGTATGGTGAATATTC+CGG | + | chr4.4:27794214-27794233 | MS.gene059529:CDS | 35.0% | |
| TACGGATTTCTACTCTATGA+TGG | + | chr4.4:27794591-27794610 | MS.gene059529:CDS | 35.0% | |
| TGATTAAAATTAGACCCTCG+CGG | - | chr4.4:27794571-27794590 | None:intergenic | 35.0% | |
| TGCTATGAACCTATAGTTCA+TGG | - | chr4.4:27794047-27794066 | None:intergenic | 35.0% | |
| TGCTCTTTGCACTTACAATT+TGG | + | chr4.4:27794092-27794111 | MS.gene059529:CDS | 35.0% | |
| TGGACTAATTTCACAGCTAA+TGG | + | chr4.4:27794112-27794131 | MS.gene059529:CDS | 35.0% | |
| TGGTTCTTCAGTGTATCATT+TGG | + | chr4.4:27794257-27794276 | MS.gene059529:CDS | 35.0% | |
| TTACCCAACACTTTGGTTAT+GGG | + | chr4.4:27794175-27794194 | MS.gene059529:CDS | 35.0% | |
| TTCAACATTACCCAACACTT+TGG | + | chr4.4:27794168-27794187 | MS.gene059529:CDS | 35.0% | |
| ! | ATTCCCATAACCAAAGTGTT+GGG | - | chr4.4:27794181-27794200 | None:intergenic | 35.0% |
| ! | TTGGTAACAGTAACACCATT+TGG | - | chr4.4:27795129-27795148 | None:intergenic | 35.0% |
| !! | TGGTGCTAAGCTTTTGATTA+TGG | + | chr4.4:27794312-27794331 | MS.gene059529:CDS | 35.0% |
| !!! | TCCGAAATTTGGCTTTTATC+CGG | + | chr4.4:27794753-27794772 | MS.gene059529:CDS | 35.0% |
| AAGAGCTTCACATGTTTGTG+TGG | + | chr4.4:27794959-27794978 | MS.gene059529:CDS | 40.0% | |
| ACAATAGAAGCAGCAGTTTC+AGG | - | chr4.4:27794346-27794365 | None:intergenic | 40.0% | |
| ACCCTTATGCCATGAACTAT+AGG | + | chr4.4:27794035-27794054 | MS.gene059529:CDS | 40.0% | |
| CATGGCATAAGGGTTATTTG+TGG | - | chr4.4:27794029-27794048 | None:intergenic | 40.0% | |
| CCACTTCTCATAGCTATGTA+TGG | + | chr4.4:27794202-27794221 | MS.gene059529:CDS | 40.0% | |
| CCATACATAGCTATGAGAAG+TGG | - | chr4.4:27794205-27794224 | None:intergenic | 40.0% | |
| GAACCACAACTTGAACCATA+AGG | - | chr4.4:27794243-27794262 | None:intergenic | 40.0% | |
| GCCGGATAAAAGCCAAATTT+CGG | - | chr4.4:27794757-27794776 | None:intergenic | 40.0% | |
| GGATTCTCTTCGAAGTTTGA+CGG | - | chr4.4:27794697-27794716 | None:intergenic | 40.0% | |
| GTAACAGTAACACCATTTGG+TGG | - | chr4.4:27795126-27795145 | None:intergenic | 40.0% | |
| TACGTAAAGTGGAACTACTG+CGG | - | chr4.4:27793885-27793904 | None:intergenic | 40.0% | |
| TCAGAAAATCAAACGCTTCC+CGG | + | chr4.4:27794464-27794483 | MS.gene059529:CDS | 40.0% | |
| TCCATCAAGCGAAACAACAT+CGG | - | chr4.4:27794386-27794405 | None:intergenic | 40.0% | |
| TGAATATTCCGGTACCCTTA+TGG | + | chr4.4:27794225-27794244 | MS.gene059529:CDS | 40.0% | |
| TGTGAAGCTCTTTAGCATCA+TGG | - | chr4.4:27794951-27794970 | None:intergenic | 40.0% | |
| ! | AACTTGAACCATAAGGGTAC+CGG | - | chr4.4:27794236-27794255 | None:intergenic | 40.0% |
| ! | GAATATTTTCCACCACCGTA+CGG | - | chr4.4:27793930-27793949 | None:intergenic | 40.0% |
| ! | GATCTTTTATGATGACTCCC+CGG | + | chr4.4:27794488-27794507 | MS.gene059529:CDS | 40.0% |
| ! | GCTCCGAGACATTCTAATTT+TGG | + | chr4.4:27794619-27794638 | MS.gene059529:CDS | 40.0% |
| ! | GTGGTGGAAAATATTCTCAC+CGG | + | chr4.4:27793934-27793953 | MS.gene059529:CDS | 40.0% |
| ! | TTTTCTTGAGACAGATGCTG+AGG | + | chr4.4:27794411-27794430 | MS.gene059529:CDS | 40.0% |
| !! | GGTGTTACTGTTACCAACAA+AGG | + | chr4.4:27795132-27795151 | MS.gene059529:CDS | 40.0% |
| !! | GTAGCCATGATTTTAGCCTA+TGG | + | chr4.4:27793902-27793921 | MS.gene059529:CDS | 40.0% |
| !! | TACCCTTATGGTTCAAGTTG+TGG | + | chr4.4:27794237-27794256 | MS.gene059529:CDS | 40.0% |
| ACTTACACTCTTGCTCAACC+CGG | - | chr4.4:27794827-27794846 | None:intergenic | 45.0% | |
| CAGCTAATGGTAGCTTAGAG+TGG | + | chr4.4:27794125-27794144 | MS.gene059529:CDS | 45.0% | |
| GCTGTGAACTCTCCGAAATT+TGG | + | chr4.4:27794742-27794761 | MS.gene059529:CDS | 45.0% | |
| GGAGCCATAGGCTAAAATCA+TGG | - | chr4.4:27793909-27793928 | None:intergenic | 45.0% | |
| TCTGATGACCATCCACCAAA+TGG | + | chr4.4:27795111-27795130 | MS.gene059529:CDS | 45.0% | |
| TTATCCGGCACAAAATGTGC+CGG | + | chr4.4:27794768-27794787 | MS.gene059529:CDS | 45.0% | |
| ! | CAGTAACACCATTTGGTGGA+TGG | - | chr4.4:27795122-27795141 | None:intergenic | 45.0% |
| ! | TTTTAGCCTATGGCTCCGTA+CGG | + | chr4.4:27793912-27793931 | MS.gene059529:CDS | 45.0% |
| !! | GGTAGTGGTGCTACTGATTA+CGG | + | chr4.4:27795030-27795049 | MS.gene059529:CDS | 45.0% |
| !! | TCCGATGTTGTTTCGCTTGA+TGG | + | chr4.4:27794382-27794401 | MS.gene059529:CDS | 45.0% |
| AAATTAGACCCTCGCGGTGT+CGG | - | chr4.4:27794565-27794584 | None:intergenic | 50.0% | |
| AACGGTTTATGCCGGAGCAT+TGG | - | chr4.4:27793961-27793980 | None:intergenic | 50.0% | |
| AACTGTGTAGAGATCGTGCC+AGG | - | chr4.4:27793858-27793877 | None:intergenic | 50.0% | |
| AAGTTTGACGGCCTTGGAGT+CGG | - | chr4.4:27794685-27794704 | None:intergenic | 50.0% | |
| CAAACCCGGAATTCTCTTCC+GGG | + | chr4.4:27794806-27794825 | MS.gene059529:CDS | 50.0% | |
| CCAAACCCGGAATTCTCTTC+CGG | + | chr4.4:27794805-27794824 | MS.gene059529:CDS | 50.0% | |
| CTCAACCCGGAAGAGAATTC+CGG | - | chr4.4:27794814-27794833 | None:intergenic | 50.0% | |
| CTGCGAAAATCGCGACGAAA+CGG | - | chr4.4:27793979-27793998 | None:intergenic | 50.0% | |
| CTTGAGACAGATGCTGAGGT+TGG | + | chr4.4:27794415-27794434 | MS.gene059529:CDS | 50.0% | |
| TCAACCCGGAAGAGAATTCC+GGG | - | chr4.4:27794813-27794832 | None:intergenic | 50.0% | |
| TCTTCGAAGTTTGACGGCCT+TGG | - | chr4.4:27794691-27794710 | None:intergenic | 50.0% | |
| ! | AGAATTCCGGGTTTGGAGCA+GGG | - | chr4.4:27794801-27794820 | None:intergenic | 50.0% |
| ! | TACGGAGCGTCTGATCAATC+TGG | + | chr4.4:27795048-27795067 | MS.gene059529:CDS | 50.0% |
| !! | GATGCTGAGGTTGGTGATGA+TGG | + | chr4.4:27794424-27794443 | MS.gene059529:CDS | 50.0% |
| !! | TTTATGCCGGAGCATTGGTC+CGG | - | chr4.4:27793956-27793975 | None:intergenic | 50.0% |
| AGCTTACCCTGCTCCAAACC+CGG | + | chr4.4:27794792-27794811 | MS.gene059529:CDS | 55.0% | |
| CAATCTGGACGCTCCGAACA+AGG | + | chr4.4:27795063-27795082 | MS.gene059529:CDS | 55.0% | |
| CCCACGGGAATTGGACTGAA+CGG | - | chr4.4:27794662-27794681 | None:intergenic | 55.0% | |
| CCGGAAGAGAATTCCGGGTT+TGG | - | chr4.4:27794808-27794827 | None:intergenic | 55.0% | |
| CCGTTCAGTCCAATTCCCGT+GGG | + | chr4.4:27794659-27794678 | MS.gene059529:CDS | 55.0% | |
| CGCGACGAAACGGTTTATGC+CGG | - | chr4.4:27793969-27793988 | None:intergenic | 55.0% | |
| GGGCTTCAAGTCTTCGGTAG+TGG | + | chr4.4:27795015-27795034 | MS.gene059529:CDS | 55.0% | |
| TCCGTTCAGTCCAATTCCCG+TGG | + | chr4.4:27794658-27794677 | MS.gene059529:CDS | 55.0% | |
| TCTGCTCCGGTGAGATTCGA+GGG | - | chr4.4:27794514-27794533 | None:intergenic | 55.0% | |
| TTCTCACCGGACCAATGCTC+CGG | + | chr4.4:27793947-27793966 | MS.gene059529:CDS | 55.0% | |
| ! | GAGAATTCCGGGTTTGGAGC+AGG | - | chr4.4:27794802-27794821 | None:intergenic | 55.0% |
| ! | TTTGGAGCAGGGTAAGCTGC+CGG | - | chr4.4:27794790-27794809 | None:intergenic | 55.0% |
| ACCGAAGACTTGAAGCCCGC+TGG | - | chr4.4:27795013-27795032 | None:intergenic | 60.0% | |
| CTCTGCTCCGGTGAGATTCG+AGG | - | chr4.4:27794515-27794534 | None:intergenic | 60.0% | |
| TAGCCTATGGCTCCGTACGG+TGG | + | chr4.4:27793915-27793934 | MS.gene059529:CDS | 60.0% | |
| ! | GCCAGCGGGCTTCAAGTCTT+CGG | + | chr4.4:27795009-27795028 | MS.gene059529:CDS | 60.0% |
| ! | GCTGCCGGCACATTTTGTGC+CGG | - | chr4.4:27794775-27794794 | None:intergenic | 60.0% |
| !! | GACTTGAAGCCCGCTGGCTT+CGG | - | chr4.4:27795007-27795026 | None:intergenic | 60.0% |
| CCACCACCGTACGGAGCCAT+AGG | - | chr4.4:27793921-27793940 | None:intergenic | 65.0% | |
| CCTATGGCTCCGTACGGTGG+TGG | + | chr4.4:27793918-27793937 | MS.gene059529:CDS | 65.0% | |
| CTCCGGTGAGATTCGAGGGC+CGG | - | chr4.4:27794510-27794529 | None:intergenic | 65.0% | |
| TCCGGTGAGATTCGAGGGCC+GGG | - | chr4.4:27794509-27794528 | None:intergenic | 65.0% | |
| TCCTGCGCCAGCTGCAACAG+CGG | + | chr4.4:27794714-27794733 | MS.gene059529:CDS | 65.0% | |
| TCGCCGGTATCCGAAGCCAG+CGG | + | chr4.4:27794994-27795013 | MS.gene059529:CDS | 65.0% | |
| TGGAGTCGGCCCACGGGAAT+TGG | - | chr4.4:27794671-27794690 | None:intergenic | 65.0% | |
| ! | ACCCTCGCGGTGTCGGATTC+CGG | - | chr4.4:27794558-27794577 | None:intergenic | 65.0% |
| ! | ATTCCCGTGGGCCGACTCCA+AGG | + | chr4.4:27794671-27794690 | MS.gene059529:CDS | 65.0% |
| ! | GTGGAGCTCTAGTGCCTCGC+CGG | + | chr4.4:27794978-27794997 | MS.gene059529:CDS | 65.0% |
| !! | AGCCCGCTGGCTTCGGATAC+CGG | - | chr4.4:27795000-27795019 | None:intergenic | 65.0% |
| ACGGCCTTGGAGTCGGCCCA+CGG | - | chr4.4:27794678-27794697 | None:intergenic | 70.0% | |
| CCCCGGCCCTCGAATCTCAC+CGG | + | chr4.4:27794505-27794524 | MS.gene059529:CDS | 70.0% | |
| CCCGGAATCCGACACCGCGA+GGG | + | chr4.4:27794554-27794573 | MS.gene059529:CDS | 70.0% | |
| CCGGTGAGATTCGAGGGCCG+GGG | - | chr4.4:27794508-27794527 | None:intergenic | 70.0% | |
| CGCCGGTATCCGAAGCCAGC+GGG | + | chr4.4:27794995-27795014 | MS.gene059529:CDS | 70.0% | |
| TCCCGGAATCCGACACCGCG+AGG | + | chr4.4:27794553-27794572 | MS.gene059529:CDS | 70.0% | |
| ! | CCCTCGCGGTGTCGGATTCC+GGG | - | chr4.4:27794557-27794576 | None:intergenic | 70.0% |
| ! | GCTGGCTTCGGATACCGGCG+AGG | - | chr4.4:27794995-27795014 | None:intergenic | 70.0% |
| CGGCCTTGGAGTCGGCCCAC+GGG | - | chr4.4:27794677-27794696 | None:intergenic | 75.0% | |
| GCCAGCTGCAACAGCGGCGC+AGG | + | chr4.4:27794720-27794739 | MS.gene059529:CDS | 75.0% | |
| GCCGCTGTTGCAGCTGGCGC+AGG | - | chr4.4:27794718-27794737 | None:intergenic | 75.0% | |
| GCCTGCGCCGCTGTTGCAGC+TGG | - | chr4.4:27794724-27794743 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 27793848 | 27795161 | 27793848 | ID=MS.gene059529 |
| chr4.4 | mRNA | 27793848 | 27795161 | 27793848 | ID=MS.gene059529.t1;Parent=MS.gene059529 |
| chr4.4 | exon | 27793848 | 27795161 | 27793848 | ID=MS.gene059529.t1.exon1;Parent=MS.gene059529.t1 |
| chr4.4 | CDS | 27793848 | 27795161 | 27793848 | ID=cds.MS.gene059529.t1;Parent=MS.gene059529.t1 |
| Gene Sequence |
| Protein sequence |
