AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29024

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29024.t1 MTR_3g073590 96.229 769 21 1 1 769 42 802 0.0 1510
MS.gene29024.t1 MTR_5g061340 69.019 581 154 3 189 768 419 974 0.0 833
MS.gene29024.t1 MTR_1g035690 41.139 474 271 6 300 768 129 599 2.66e-121 377
MS.gene29024.t1 MTR_8g033290 41.314 472 272 5 299 768 154 622 3.05e-119 372
MS.gene29024.t1 MTR_8g078760 30.734 218 140 3 560 769 404 618 1.37e-22 103
MS.gene29024.t1 MTR_5g018820 36.000 150 89 1 557 699 398 547 1.46e-20 97.1
MS.gene29024.t1 MTR_8g079840 34.682 173 101 5 560 726 379 545 4.84e-19 92.0
MS.gene29024.t1 MTR_3g101340 32.184 174 104 3 560 726 321 487 1.80e-18 90.1
MS.gene29024.t1 MTR_3g101340 32.184 174 104 3 560 726 347 513 1.82e-18 90.1
MS.gene29024.t1 MTR_5g066810 68.519 54 17 0 715 768 10 63 2.71e-18 79.7
MS.gene29024.t1 MTR_3g101340 30.220 182 105 3 560 726 346 520 3.21e-18 89.4
MS.gene29024.t1 MTR_4g068920 34.211 152 93 2 556 700 407 558 1.17e-17 87.8
MS.gene29024.t1 MTR_5g017790 34.118 170 100 5 563 726 331 494 2.98e-17 86.3
MS.gene29024.t1 MTR_5g017790 30.693 202 127 6 532 726 287 482 3.63e-17 85.9
MS.gene29024.t1 MTR_1g021040 31.548 168 106 3 563 726 333 495 4.48e-17 85.9
MS.gene29024.t1 MTR_8g078760 36.800 125 72 1 560 677 404 528 2.64e-16 83.2
MS.gene29024.t1 MTR_5g012790 31.765 170 101 3 547 703 345 512 1.09e-15 81.6
MS.gene29024.t1 MTR_5g079910 30.729 192 125 4 556 744 289 475 1.24e-13 74.7
MS.gene29024.t1 MTR_4g058015 27.706 231 145 7 556 769 462 687 4.32e-13 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29024.t1 AT4G08550 62.522 571 192 4 200 768 140 690 0.0 720
MS.gene29024.t1 AT4G08550 55.692 571 177 6 200 768 140 636 0.0 608
MS.gene29024.t1 AT3G11920 39.413 477 280 5 299 768 155 629 6.14e-116 364
MS.gene29024.t1 AT5G66600 33.721 172 105 2 560 725 371 539 1.18e-23 106
MS.gene29024.t1 AT5G66600 33.721 172 105 2 560 725 337 505 1.23e-23 106
MS.gene29024.t1 AT5G66600 33.721 172 105 2 560 725 391 559 1.23e-23 106
MS.gene29024.t1 AT5G66600 33.721 172 105 2 560 725 391 559 1.23e-23 106
MS.gene29024.t1 AT5G66600 33.721 172 105 2 560 725 391 559 1.23e-23 106
MS.gene29024.t1 AT5G66600 33.721 172 105 2 560 725 391 559 1.23e-23 106
MS.gene29024.t1 AT5G66600 33.721 172 105 2 560 725 391 559 1.23e-23 106
MS.gene29024.t1 AT1G16750 30.081 246 134 7 549 769 293 525 6.27e-23 103
MS.gene29024.t1 AT3G13000 31.280 211 124 7 549 747 314 515 7.35e-22 100
MS.gene29024.t1 AT3G13000 31.280 211 124 7 549 747 343 544 9.63e-22 100
MS.gene29024.t1 AT3G13000 31.280 211 124 7 549 747 343 544 9.63e-22 100
MS.gene29024.t1 AT2G23700 30.594 219 140 5 560 769 484 699 6.25e-20 95.5
MS.gene29024.t1 AT5G66600 38.710 124 70 1 560 677 391 514 1.81e-18 90.1
MS.gene29024.t1 AT5G66600 38.710 124 70 1 560 677 391 514 1.81e-18 90.1
MS.gene29024.t1 AT5G66600 38.710 124 70 1 560 677 391 514 1.81e-18 90.1
MS.gene29024.t1 AT5G66600 38.710 124 70 1 560 677 391 514 1.81e-18 90.1
MS.gene29024.t1 AT4G37080 32.948 173 104 5 560 726 392 558 3.28e-18 89.7
MS.gene29024.t1 AT4G37080 32.948 173 104 5 560 726 392 558 3.28e-18 89.7
MS.gene29024.t1 AT4G37080 32.948 173 104 5 560 726 379 545 4.50e-18 89.0
MS.gene29024.t1 AT5G42690 32.558 172 106 4 559 726 321 486 1.19e-17 87.4
MS.gene29024.t1 AT5G42690 32.558 172 106 4 559 726 322 487 1.22e-17 87.4
MS.gene29024.t1 AT5G42690 32.558 172 106 4 559 726 322 487 1.22e-17 87.4
MS.gene29024.t1 AT5G42690 32.558 172 106 4 559 726 295 460 1.23e-17 87.4
MS.gene29024.t1 AT5G42690 32.558 172 106 4 559 726 270 435 1.30e-17 87.0
MS.gene29024.t1 AT5G47380 34.682 173 96 4 557 717 359 526 2.67e-17 86.7
MS.gene29024.t1 AT5G47380 34.682 173 96 4 557 717 303 470 3.25e-17 86.3
MS.gene29024.t1 AT5G47380 33.898 177 100 4 557 721 359 530 3.27e-17 86.3
MS.gene29024.t1 AT3G18900 31.098 164 105 3 544 699 214 377 4.88e-16 82.0
MS.gene29024.t1 AT3G18900 31.098 164 105 3 544 699 214 377 4.88e-16 82.0
MS.gene29024.t1 AT3G18900 31.098 164 105 3 544 699 295 458 7.20e-16 82.0
MS.gene29024.t1 AT3G18900 31.098 164 105 3 544 699 290 453 7.21e-16 82.0
MS.gene29024.t1 AT1G21060 26.957 230 153 6 549 769 266 489 5.88e-14 75.9
MS.gene29024.t1 AT1G21060 26.957 230 153 6 549 769 278 501 7.32e-14 75.5
MS.gene29024.t1 AT1G76620 33.553 152 86 4 560 700 299 446 2.12e-13 73.9
MS.gene29024.t1 AT2G39690 30.952 168 110 3 560 725 307 470 5.65e-13 72.8
MS.gene29024.t1 AT2G39690 30.952 168 110 3 560 725 281 444 7.23e-13 72.4
MS.gene29024.t1 AT2G39690 30.952 168 110 3 560 725 200 363 7.27e-13 71.6
MS.gene29024.t1 AT2G39690 30.357 168 110 3 560 725 307 469 1.62e-12 71.2
MS.gene29024.t1 AT2G39690 30.357 168 110 3 560 725 281 443 1.70e-12 70.9
MS.gene29024.t1 AT1G43020 32.667 150 87 5 560 699 218 363 2.99e-12 70.1
MS.gene29024.t1 AT3G18900 30.986 142 90 3 544 677 295 436 3.03e-12 70.1
MS.gene29024.t1 AT5G47380 35.338 133 71 3 557 677 359 488 5.07e-12 69.7
MS.gene29024.t1 AT5G47380 35.878 131 69 3 557 675 359 486 5.73e-12 69.3
MS.gene29024.t1 AT1G16750 37.374 99 59 1 549 644 293 391 5.82e-12 68.9

Find 173 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACTTCTTAATATAATTTA+AGG 0.073218 3.4:-72232066 None:intergenic
TGCATCAAGGCTGAGATTTC+TGG 0.101988 3.4:+72233431 MS.gene29024:CDS
TGCGATAGGCTTTGGATTAA+TGG 0.164279 3.4:-72233403 None:intergenic
TTGGAAAGAAGGAAATTATT+TGG 0.198959 3.4:+72233801 MS.gene29024:CDS
TTCAATGTCACTGGAGATTC+TGG 0.202694 3.4:+72231507 MS.gene29024:CDS
TTTAGGTATAGCATAGATTT+TGG 0.208784 3.4:+72234581 MS.gene29024:intron
TCCAAGTGAGGACAACAATT+TGG 0.230260 3.4:+72231743 MS.gene29024:CDS
CATCCATGCAATCTTAATAT+TGG 0.235219 3.4:+72233761 MS.gene29024:CDS
CTTATCAGAAGATCAATATT+TGG 0.236747 3.4:+72232610 MS.gene29024:CDS
AATCCAATATTTCAACTCTT+TGG 0.237090 3.4:-72233686 None:intergenic
ACTATGCTGCGCCGCCTTTC+TGG 0.241084 3.4:+72231921 MS.gene29024:CDS
TCCCTTTCAGCTATTCAAAA+TGG 0.243224 3.4:+72233858 MS.gene29024:CDS
AACTGACTCAGCAATATTAT+TGG 0.243565 3.4:+72234652 MS.gene29024:CDS
GGAGAGTTCAAATATGTTAT+AGG 0.244778 3.4:+72233822 MS.gene29024:CDS
TTGGTATGTGGCACCCGATC+TGG 0.254719 3.4:+72234116 MS.gene29024:CDS
AATGGCTTCTTAAGGTTATA+TGG 0.266212 3.4:-72233906 None:intergenic
TGCAAAAGATAAACGCTTAA+AGG 0.270667 3.4:+72233932 MS.gene29024:CDS
GAGCACATCTACCCGTAAAT+TGG 0.283924 3.4:+72231812 MS.gene29024:CDS
CCTGATCTCCATCCAATTTA+CGG 0.285251 3.4:-72231824 None:intergenic
CTTCTTTCCAATGCTCCATC+TGG 0.285483 3.4:-72233789 None:intergenic
CAGTATAATCCTTCCTTTCA+TGG 0.286548 3.4:-72232159 None:intergenic
ATCCTTCCTTTCATGGCTAT+TGG 0.292169 3.4:-72232152 None:intergenic
AATTTGGACCCTGTATTTGA+TGG 0.308860 3.4:+72231759 MS.gene29024:CDS
GCTCCAAAGAGTTGAAATAT+TGG 0.318765 3.4:+72233683 MS.gene29024:CDS
ATATCATCCTCTCCAGAAAA+AGG 0.320824 3.4:-72232455 None:intergenic
GCAATTTCTGCGATAGGCTT+TGG 0.331252 3.4:-72233411 None:intergenic
TTCATCAACTGCCCCACTAA+TGG 0.342890 3.4:-72232480 None:intergenic
ATTTGATGGAACCGAGGTTC+CGG 0.359300 3.4:+72231773 MS.gene29024:CDS
GCTTGCCTCTTCTGAATTGA+AGG 0.361482 3.4:+72234679 MS.gene29024:CDS
ACGGAGTTGGAGAAGATATC+TGG 0.363743 3.4:+72232287 MS.gene29024:CDS
CTAGATGAAATGCACAGATA+TGG 0.373544 3.4:+72231642 MS.gene29024:CDS
ATCCATGCAATCTTAATATT+GGG 0.374358 3.4:+72233762 MS.gene29024:CDS
GACTTCTTAATATAATTTAA+GGG 0.376502 3.4:-72232065 None:intergenic
TAACATTGTGACATCACAAT+TGG 0.376676 3.4:-72233357 None:intergenic
TTTGATGGAACCGAGGTTCC+GGG 0.376989 3.4:+72231774 MS.gene29024:CDS
AGATTCTTGACACCCTAGTT+TGG 0.383221 3.4:-72232186 None:intergenic
TCCAAATTGTTGTCCTCACT+TGG 0.389670 3.4:-72231744 None:intergenic
TGCCATTCAGATTGAAGATC+AGG 0.390522 3.4:+72231353 MS.gene29024:CDS
ATGTCAGTAAGATTCTTAAA+TGG 0.394125 3.4:+72234252 MS.gene29024:CDS
TGGTATGTGGCACCCGATCT+GGG 0.395261 3.4:+72234117 MS.gene29024:CDS
AGAAGTTAATGAAGCCAAAC+TGG 0.400357 3.4:+72232007 MS.gene29024:CDS
GAGGATGATATTTCCATTAG+TGG 0.406602 3.4:+72232467 MS.gene29024:CDS
CAGATATGGACTGGATGATT+TGG 0.409841 3.4:+72231656 MS.gene29024:CDS
AGTGAAAATTTATTTGAAGA+TGG 0.410745 3.4:+72233303 MS.gene29024:intron
CCCTAAGTTTGACAGTAAAC+TGG 0.417217 3.4:+72231413 MS.gene29024:CDS
GTCAAGAATCTAGAATATTT+AGG 0.417224 3.4:+72232198 MS.gene29024:CDS
TCCAACTCCGTCTTTCTACC+AGG 0.418763 3.4:-72232275 None:intergenic
TAGTGGGGCAGTTGATGAAT+TGG 0.419111 3.4:+72232484 MS.gene29024:CDS
TTGCACCGAATGGCTTCTTA+AGG 0.419522 3.4:-72233914 None:intergenic
TGGCTTCTCAGAAACCAGTT+TGG 0.424844 3.4:-72232021 None:intergenic
AGAGTATTCTGGTGGTGATA+AGG 0.425577 3.4:+72231575 MS.gene29024:CDS
TTCATGGCTATTGGTGATGA+TGG 0.432688 3.4:-72232143 None:intergenic
TCCAGTTTACTGTCAAACTT+AGG 0.433560 3.4:-72231414 None:intergenic
ACCTCGGTTCCATCAAATAC+AGG 0.434676 3.4:-72231768 None:intergenic
ACCAAAGCAAAATGAATCAT+AGG 0.439045 3.4:-72234098 None:intergenic
CTGATCTCCATCCAATTTAC+GGG 0.439424 3.4:-72231823 None:intergenic
TCACGAGACCTCCACTTCTC+AGG 0.439974 3.4:-72234205 None:intergenic
TTCCAAGCAGATTTCTCCAC+TGG 0.442084 3.4:-72232041 None:intergenic
ATTATACTGTACACCAAACT+AGG 0.448111 3.4:+72232173 MS.gene29024:CDS
AATGTTGGTAAAGAGTATTC+TGG 0.448696 3.4:+72231564 MS.gene29024:CDS
GCCCTTCGATGCTATTCTCC+TGG 0.451401 3.4:+72234143 MS.gene29024:CDS
CCGTAAATTGGATGGAGATC+AGG 0.452393 3.4:+72231824 MS.gene29024:CDS
GCAAAATGAATCATAGGCTC+GGG 0.452440 3.4:-72234092 None:intergenic
CAGATGGAGCATTGGAAAGA+AGG 0.457598 3.4:+72233790 MS.gene29024:CDS
TTCCGGGGATGCAAGTTAAC+CGG 0.460048 3.4:+72231790 MS.gene29024:CDS
TTAATGGTAATAATACCCTT+TGG 0.464994 3.4:-72233387 None:intergenic
TTCAATGAAATATGTATTGG+AGG 0.465319 3.4:+72232335 MS.gene29024:CDS
TGATACATCGGACGTGATAA+CGG 0.466719 3.4:+72231437 MS.gene29024:CDS
AGACATGGAATCCACAGATA+TGG 0.468766 3.4:+72231701 MS.gene29024:CDS
TCGAAGGGCAGGCCCAGATC+GGG 0.471322 3.4:-72234129 None:intergenic
CTCCGGTTAACTTGCATCCC+CGG 0.471561 3.4:-72231792 None:intergenic
CAATATTAAGATTGCATGGA+TGG 0.471965 3.4:-72233760 None:intergenic
TTTAGCCTTAAAATTTATGT+TGG 0.474983 3.4:-72231604 None:intergenic
ATCGAAGGGCAGGCCCAGAT+CGG 0.479385 3.4:-72234130 None:intergenic
ATCCTGATCTTCAATCTGAA+TGG 0.479600 3.4:-72231355 None:intergenic
CTTGATGCAATTTCTGCGAT+AGG 0.479925 3.4:-72233417 None:intergenic
AGGTCTCGTGATTGATTTGA+CGG 0.480468 3.4:+72234217 MS.gene29024:CDS
AGGATGATATTTCCATTAGT+GGG 0.486028 3.4:+72232468 MS.gene29024:CDS
AGCAAAATGAATCATAGGCT+CGG 0.489457 3.4:-72234093 None:intergenic
TGAATCATAGGCTCGGGGTA+AGG 0.490288 3.4:-72234086 None:intergenic
TAGTTCAAACCCCAGTCATA+AGG 0.490858 3.4:-72234713 None:intergenic
TTGCAGCTTCTCAACCGCTT+GGG 0.498671 3.4:-72231319 None:intergenic
TTGCATGGAAGTGAGGAGTT+CGG 0.506332 3.4:+72233513 MS.gene29024:CDS
CATGGAAGATCAGCATTTGT+TGG 0.507056 3.4:+72231278 MS.gene29024:CDS
AATAATATTGCTGAGTCAGT+TGG 0.507212 3.4:-72234650 None:intergenic
GAAGCCTATGCTATTGAAGA+TGG 0.508503 3.4:+72233468 MS.gene29024:CDS
CAGTCCAGGTGTGGTTGAGA+AGG 0.509358 3.4:+72231848 MS.gene29024:CDS
GAATCATAGGCTCGGGGTAA+GGG 0.509661 3.4:-72234085 None:intergenic
CTTGCAGCTTCTCAACCGCT+TGG 0.510135 3.4:-72231320 None:intergenic
GGAGAATAGCATCGAAGGGC+AGG 0.510556 3.4:-72234140 None:intergenic
CAGAGGGATTCAGTAACAAA+TGG 0.513968 3.4:+72232119 MS.gene29024:CDS
CAACAAATGCTGATCTTCCA+TGG 0.517186 3.4:-72231277 None:intergenic
ACTGCCTTCTCAACCACACC+TGG 0.521661 3.4:-72231852 None:intergenic
TTACATATCAGCCTTATGAC+TGG 0.523585 3.4:+72234702 MS.gene29024:CDS
CCAGTTTACTGTCAAACTTA+GGG 0.524030 3.4:-72231413 None:intergenic
TATTCAAAATGGAATATTGA+GGG 0.525670 3.4:+72233869 MS.gene29024:CDS
TAAGGTTATATGGAGGTCGT+TGG 0.529342 3.4:-72233896 None:intergenic
TGGAAAACAAAACTGATCAG+AGG 0.529835 3.4:+72232102 MS.gene29024:CDS
TCTCAGAATGTTGTTCATGA+TGG 0.530719 3.4:+72231390 MS.gene29024:CDS
TACATATCAGCCTTATGACT+GGG 0.530867 3.4:+72234703 MS.gene29024:CDS
CAGAAGATCAATATTTGGAA+AGG 0.531565 3.4:+72232615 MS.gene29024:CDS
TCAGCATTTGTTGGAGACAA+AGG 0.531880 3.4:+72231287 MS.gene29024:CDS
GACCCAATATTAAGATTGCA+TGG 0.533239 3.4:-72233764 None:intergenic
TCAATTCAGAAGAGGCAAGC+AGG 0.533585 3.4:-72234676 None:intergenic
AACTTGCATCCCCGGAACCT+CGG 0.534932 3.4:-72231784 None:intergenic
AGAAAGGCGGCGCAGCATAG+TGG 0.536077 3.4:-72231919 None:intergenic
CACCAATAGCCATGAAAGGA+AGG 0.542926 3.4:+72232150 MS.gene29024:CDS
CGCAATTTCCTGAGAAGTGG+AGG 0.544921 3.4:+72234197 MS.gene29024:CDS
GATTGAAGATCAGGATAAAG+AGG 0.545716 3.4:+72231362 MS.gene29024:CDS
ATTTCAGTTTCAGAATTGAT+CGG 0.549208 3.4:-72231537 None:intergenic
GGTGATGGGGTTTCAAATAA+CGG 0.551236 3.4:-72232431 None:intergenic
ATCCACAGATATGGATCAGA+AGG 0.552063 3.4:+72231710 MS.gene29024:CDS
AGCCAGTGGAGAAATCTGCT+TGG 0.553500 3.4:+72232039 MS.gene29024:CDS
TTCCAGGAGAATAGCATCGA+AGG 0.554025 3.4:-72234145 None:intergenic
GTGATAACGGATGACAATGA+TGG 0.554075 3.4:+72231450 MS.gene29024:CDS
CAATGTTACAATATTCCAAA+GGG 0.554541 3.4:+72233372 MS.gene29024:CDS
AAACAACTTGGGAACAGAAG+TGG 0.555017 3.4:-72232312 None:intergenic
GATGGAGATCAGGACAGTCC+AGG 0.555381 3.4:+72231834 MS.gene29024:CDS
GTATTCTGGTGGTGATAAGG+TGG 0.556223 3.4:+72231578 MS.gene29024:CDS
TTGAATAGCTGAAAGGGAGT+AGG 0.559780 3.4:-72233853 None:intergenic
GAAGTCATTTGATGTTGATG+TGG 0.561143 3.4:+72232082 MS.gene29024:CDS
TATAACCTTAAGAAGCCATT+CGG 0.563687 3.4:+72233909 MS.gene29024:CDS
CCCTGGTAGAAAGACGGAGT+TGG 0.563695 3.4:+72232274 MS.gene29024:CDS
TTAATATTGGGTCATCCAGA+TGG 0.565815 3.4:+72233774 MS.gene29024:CDS
ACAGATTGTACAAAATGCGC+AGG 0.567629 3.4:+72232549 MS.gene29024:CDS
TGCCTTCTGATCCATATCTG+TGG 0.567944 3.4:-72231712 None:intergenic
GAAACTGAAATTGATAATGT+TGG 0.569198 3.4:+72231549 MS.gene29024:CDS
ATTTACGGGTAGATGTGCTC+CGG 0.569546 3.4:-72231809 None:intergenic
TACAAGTTTGCATGGAAGTG+AGG 0.571856 3.4:+72233506 MS.gene29024:CDS
ATGGAAGTGAGGAGTTCGGC+AGG 0.573905 3.4:+72233517 MS.gene29024:CDS
AATAGCTGAAAGGGAGTAGG+TGG 0.575549 3.4:-72233850 None:intergenic
ACTCGCAATTTCCTGAGAAG+TGG 0.578546 3.4:+72234194 MS.gene29024:CDS
TTCTGGTGGTGATAAGGTGG+AGG 0.580331 3.4:+72231581 MS.gene29024:CDS
TCCAGGAGAATAGCATCGAA+GGG 0.585737 3.4:-72234144 None:intergenic
CTATTCAAAATGGAATATTG+AGG 0.586041 3.4:+72233868 MS.gene29024:CDS
ACAATGTTACAATATTCCAA+AGG 0.587793 3.4:+72233371 MS.gene29024:CDS
TGAAATGCACAGATATGGAC+TGG 0.589172 3.4:+72231647 MS.gene29024:CDS
ACCATAGCTTGCTATCGCCA+TGG 0.589422 3.4:+72231260 None:intergenic
TGTAACCTTCAATTCAGAAG+AGG 0.594840 3.4:-72234684 None:intergenic
GGAAAACAAAACTGATCAGA+GGG 0.600988 3.4:+72232103 MS.gene29024:CDS
GTTGATTATACAAGTTTGCA+TGG 0.601412 3.4:+72233498 MS.gene29024:CDS
TCAATATTTGGAAAGGCTAG+AGG 0.606043 3.4:+72232622 MS.gene29024:CDS
TCATCACCAATAGCCATGAA+AGG 0.614646 3.4:+72232146 MS.gene29024:CDS
CCAACTCCGTCTTTCTACCA+GGG 0.616219 3.4:-72232274 None:intergenic
ACATCTACCCGTAAATTGGA+TGG 0.616547 3.4:+72231816 MS.gene29024:CDS
ACATATCAGCCTTATGACTG+GGG 0.619604 3.4:+72234704 MS.gene29024:CDS
GAGTTCAAATATGTTATAGG+TGG 0.620548 3.4:+72233825 MS.gene29024:CDS
GGGTCATCCAGATGGAGCAT+TGG 0.621494 3.4:+72233782 MS.gene29024:CDS
TTGATGGAACCGAGGTTCCG+GGG 0.624334 3.4:+72231775 MS.gene29024:CDS
GGCAGATGAAATTCCAAGTG+AGG 0.627307 3.4:+72231731 MS.gene29024:CDS
CCCTGTATTTGATGGAACCG+AGG 0.628835 3.4:+72231767 MS.gene29024:CDS
TTATACTGTACACCAAACTA+GGG 0.629182 3.4:+72232174 MS.gene29024:CDS
GCATCCATCTTCAATAGCAT+AGG 0.630339 3.4:-72233472 None:intergenic
CTATCGCAGAAATTGCATCA+AGG 0.631626 3.4:+72233418 MS.gene29024:CDS
GGATGATATTTCCATTAGTG+GGG 0.635196 3.4:+72232469 MS.gene29024:CDS
CAGTAAACTGGATGATACAT+CGG 0.636432 3.4:+72231425 MS.gene29024:CDS
TGTATACCCTGGTAGAAAGA+CGG 0.637748 3.4:+72232268 MS.gene29024:CDS
ATTCAAAATGGAATATTGAG+GGG 0.652429 3.4:+72233870 MS.gene29024:CDS
CCTCGGTTCCATCAAATACA+GGG 0.653158 3.4:-72231767 None:intergenic
AGAGAACAACGGTAGAGACA+TGG 0.654893 3.4:+72231686 MS.gene29024:CDS
AGATCAGGACAGTCCAGGTG+TGG 0.655626 3.4:+72231839 MS.gene29024:CDS
GGCTTCTTAAGGTTATATGG+AGG 0.664268 3.4:-72233903 None:intergenic
ATCAACATAGATGTATACCC+TGG 0.667136 3.4:+72232257 MS.gene29024:CDS
TAACGGATGACAATGATGGA+AGG 0.676865 3.4:+72231454 MS.gene29024:CDS
CAAAATGAATCATAGGCTCG+GGG 0.678249 3.4:-72234091 None:intergenic
ACTGGTTTCTGAGAAGCCAG+TGG 0.679919 3.4:+72232025 MS.gene29024:CDS
TGTAAAGGAGAAAAGTGCAG+TGG 0.690641 3.4:+72231890 MS.gene29024:CDS
AAAAGTGATGAAGCTGCTGT+GGG 0.696516 3.4:+72231945 MS.gene29024:CDS
ATAGGCTTCGAACATTGCAG+AGG 0.705917 3.4:-72233454 None:intergenic
AAAGGAAGCAAGTTCCCAAG+CGG 0.717434 3.4:+72231305 MS.gene29024:CDS
TTGGCTAAAGCAGAGAACAA+CGG 0.720989 3.4:+72231675 MS.gene29024:CDS
GTTGGTAAAGAGTATTCTGG+TGG 0.735908 3.4:+72231567 MS.gene29024:CDS
GCAAACTTGTATAATCAACG+CGG 0.798022 3.4:-72233494 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAGTATAGAATTTTAAA+AGG - chr3.4:72234357-72234376 None:intergenic 10.0%
!! ATAATGTATTACATAAAATG+AGG + chr3.4:72232827-72232846 MS.gene29024:intron 15.0%
!! GACTTCTTAATATAATTTAA+GGG - chr3.4:72232068-72232087 None:intergenic 15.0%
!! TAAAAAAAGAAGAAAAACAA+TGG - chr3.4:72233117-72233136 None:intergenic 15.0%
!! TGACTTCTTAATATAATTTA+AGG - chr3.4:72232069-72232088 None:intergenic 15.0%
!!! ATATGATCTTTTAAATCTAT+GGG - chr3.4:72232971-72232990 None:intergenic 15.0%
!!! ATTAGTAAATATTGTTACTT+TGG - chr3.4:72233269-72233288 None:intergenic 15.0%
!!! TATATGATCTTTTAAATCTA+TGG - chr3.4:72232972-72232991 None:intergenic 15.0%
!!! TTTTTCAATGAAATATGTAT+TGG + chr3.4:72232332-72232351 MS.gene29024:CDS 15.0%
!! AAAAATTGACGAAGAATTAT+TGG + chr3.4:72234166-72234185 MS.gene29024:CDS 20.0%
!! AGTGAAAATTTATTTGAAGA+TGG + chr3.4:72233303-72233322 MS.gene29024:intron 20.0%
!! ATTTCATTGAAAAACAACTT+GGG - chr3.4:72232326-72232345 None:intergenic 20.0%
!! TATTCAAAATGGAATATTGA+GGG + chr3.4:72233869-72233888 MS.gene29024:CDS 20.0%
!! TATTTCATTGAAAAACAACT+TGG - chr3.4:72232327-72232346 None:intergenic 20.0%
!! TTAAATCTATGGGATATTTA+GGG - chr3.4:72232961-72232980 None:intergenic 20.0%
!! TTCTTTCAAATACAATTTGT+AGG + chr3.4:72232907-72232926 MS.gene29024:intron 20.0%
!! TTTAAATCTATGGGATATTT+AGG - chr3.4:72232962-72232981 None:intergenic 20.0%
!!! CTTCTTTTTTTAATTTTGTC+TGG + chr3.4:72233125-72233144 MS.gene29024:intron 20.0%
!!! TTTAGCCTTAAAATTTATGT+TGG - chr3.4:72231607-72231626 None:intergenic 20.0%
! AATCCAATATTTCAACTCTT+TGG - chr3.4:72233689-72233708 None:intergenic 25.0%
! ACAATGTTACAATATTCCAA+AGG + chr3.4:72233371-72233390 MS.gene29024:CDS 25.0%
! ATACCTAAATATGAACAATG+AGG - chr3.4:72234570-72234589 None:intergenic 25.0%
! ATCCATGCAATCTTAATATT+GGG + chr3.4:72233762-72233781 MS.gene29024:CDS 25.0%
! ATGTCAGTAAGATTCTTAAA+TGG + chr3.4:72234252-72234271 MS.gene29024:CDS 25.0%
! ATTCAAAATGGAATATTGAG+GGG + chr3.4:72233870-72233889 MS.gene29024:CDS 25.0%
! ATTCCTCATTGTTCATATTT+AGG + chr3.4:72234564-72234583 MS.gene29024:intron 25.0%
! ATTTCAGTTTCAGAATTGAT+CGG - chr3.4:72231540-72231559 None:intergenic 25.0%
! CAATGTTACAATATTCCAAA+GGG + chr3.4:72233372-72233391 MS.gene29024:CDS 25.0%
! CTATTCAAAATGGAATATTG+AGG + chr3.4:72233868-72233887 MS.gene29024:CDS 25.0%
! CTTATCAGAAGATCAATATT+TGG + chr3.4:72232610-72232629 MS.gene29024:CDS 25.0%
! GAAAAAAAATTCAATGTCAC+TGG + chr3.4:72231498-72231517 MS.gene29024:CDS 25.0%
! GTTAGAAATTGTGTTTACTT+TGG + chr3.4:72234518-72234537 MS.gene29024:intron 25.0%
! TGCTAGTGTATAAGATATTA+AGG - chr3.4:72234306-72234325 None:intergenic 25.0%
! TTAATGGTAATAATACCCTT+TGG - chr3.4:72233390-72233409 None:intergenic 25.0%
! TTCAATGAAATATGTATTGG+AGG + chr3.4:72232335-72232354 MS.gene29024:CDS 25.0%
! TTGGAAAGAAGGAAATTATT+TGG + chr3.4:72233801-72233820 MS.gene29024:CDS 25.0%
! TTTGATTACGTATGAATTTG+TGG - chr3.4:72232710-72232729 None:intergenic 25.0%
!! GTCAAGAATCTAGAATATTT+AGG + chr3.4:72232198-72232217 MS.gene29024:CDS 25.0%
!! TTTAGGTATAGCATAGATTT+TGG + chr3.4:72234581-72234600 MS.gene29024:intron 25.0%
!! TTTTACGTACAAAAAGACTT+AGG + chr3.4:72232225-72232244 MS.gene29024:CDS 25.0%
!!! CGTTGATTCATTTATTTGAT+AGG + chr3.4:72234055-72234074 MS.gene29024:intron 25.0%
!!! CTTTTTTTAATTTTGTCTGG+TGG + chr3.4:72233128-72233147 MS.gene29024:intron 25.0%
!!! GAAACTGAAATTGATAATGT+TGG + chr3.4:72231549-72231568 MS.gene29024:CDS 25.0%
!!! TTTCGAGCACAATTTATTTT+TGG + chr3.4:72233081-72233100 MS.gene29024:intron 25.0%
AATAATATTGCTGAGTCAGT+TGG - chr3.4:72234653-72234672 None:intergenic 30.0%
AATGGCTTCTTAAGGTTATA+TGG - chr3.4:72233909-72233928 None:intergenic 30.0%
ACCAAAGCAAAATGAATCAT+AGG - chr3.4:72234101-72234120 None:intergenic 30.0%
AGGATGATATTTCCATTAGT+GGG + chr3.4:72232468-72232487 MS.gene29024:CDS 30.0%
ATTATACTGTACACCAAACT+AGG + chr3.4:72232173-72232192 MS.gene29024:CDS 30.0%
CAATATTAAGATTGCATGGA+TGG - chr3.4:72233763-72233782 None:intergenic 30.0%
CAGAAGATCAATATTTGGAA+AGG + chr3.4:72232615-72232634 MS.gene29024:CDS 30.0%
CATCCATGCAATCTTAATAT+TGG + chr3.4:72233761-72233780 MS.gene29024:CDS 30.0%
CGCTCTTAAAAATCTTGTAA+AGG + chr3.4:72231875-72231894 MS.gene29024:CDS 30.0%
CTTAAAAACTTGGATGAATC+TGG + chr3.4:72232368-72232387 MS.gene29024:CDS 30.0%
GAACTTACAAAATGACTGAA+TGG - chr3.4:72233618-72233637 None:intergenic 30.0%
GAGTTCAAATATGTTATAGG+TGG + chr3.4:72233825-72233844 MS.gene29024:CDS 30.0%
GGAGAGTTCAAATATGTTAT+AGG + chr3.4:72233822-72233841 MS.gene29024:CDS 30.0%
GTTGATTATACAAGTTTGCA+TGG + chr3.4:72233498-72233517 MS.gene29024:CDS 30.0%
TAACATTGTGACATCACAAT+TGG - chr3.4:72233360-72233379 None:intergenic 30.0%
TACAAAAAGACTTAGGTTTG+TGG + chr3.4:72232232-72232251 MS.gene29024:CDS 30.0%
TATAACCTTAAGAAGCCATT+CGG + chr3.4:72233909-72233928 MS.gene29024:CDS 30.0%
TGCAAAAGATAAACGCTTAA+AGG + chr3.4:72233932-72233951 MS.gene29024:CDS 30.0%
TTATACTGTACACCAAACTA+GGG + chr3.4:72232174-72232193 MS.gene29024:CDS 30.0%
TTGTCTAATTGTCTTAGCTT+TGG + chr3.4:72232650-72232669 MS.gene29024:intron 30.0%
! AACTGACTCAGCAATATTAT+TGG + chr3.4:72234652-72234671 MS.gene29024:CDS 30.0%
! AATTCTTCGTCAATTTTTCC+AGG - chr3.4:72234164-72234183 None:intergenic 30.0%
! ATGCTATTTGTTGACATTGT+AGG + chr3.4:72233640-72233659 MS.gene29024:intron 30.0%
! ATTCATTTTGCTTTGGTATG+TGG + chr3.4:72234104-72234123 MS.gene29024:CDS 30.0%
! TCCATTTTGAATAGCTGAAA+GGG - chr3.4:72233862-72233881 None:intergenic 30.0%
! TGTCTAATTGTCTTAGCTTT+GGG + chr3.4:72232651-72232670 MS.gene29024:intron 30.0%
! TTCCATTTTGAATAGCTGAA+AGG - chr3.4:72233863-72233882 None:intergenic 30.0%
!! AATGTTGGTAAAGAGTATTC+TGG + chr3.4:72231564-72231583 MS.gene29024:CDS 30.0%
!! TCAGAGTTCTAAACTTCTAA+CGG - chr3.4:72233572-72233591 None:intergenic 30.0%
!!! AGGTTTACCAACTGTTTTTT+GGG + chr3.4:72232569-72232588 MS.gene29024:CDS 30.0%
!!! CTTTTGTTGTTGTTGTTGTT+TGG + chr3.4:72233962-72233981 MS.gene29024:intron 30.0%
AATTTGGACCCTGTATTTGA+TGG + chr3.4:72231759-72231778 MS.gene29024:CDS 35.0%
AGAAGTTAATGAAGCCAAAC+TGG + chr3.4:72232007-72232026 MS.gene29024:CDS 35.0%
AGCAAAATGAATCATAGGCT+CGG - chr3.4:72234096-72234115 None:intergenic 35.0%
ATATCATCCTCTCCAGAAAA+AGG - chr3.4:72232458-72232477 None:intergenic 35.0%
ATCAACATAGATGTATACCC+TGG + chr3.4:72232257-72232276 MS.gene29024:CDS 35.0%
ATCCTGATCTTCAATCTGAA+TGG - chr3.4:72231358-72231377 None:intergenic 35.0%
ATTGTGCTCGAAAGAATCTA+TGG - chr3.4:72233074-72233093 None:intergenic 35.0%
CAGTAAACTGGATGATACAT+CGG + chr3.4:72231425-72231444 MS.gene29024:CDS 35.0%
CAGTATAATCCTTCCTTTCA+TGG - chr3.4:72232162-72232181 None:intergenic 35.0%
CCAGTTTACTGTCAAACTTA+GGG - chr3.4:72231416-72231435 None:intergenic 35.0%
CTAGATGAAATGCACAGATA+TGG + chr3.4:72231642-72231661 MS.gene29024:CDS 35.0%
CTTGAGTGAGCTTAAAAACT+TGG + chr3.4:72232358-72232377 MS.gene29024:CDS 35.0%
GAAGTCATTTGATGTTGATG+TGG + chr3.4:72232082-72232101 MS.gene29024:CDS 35.0%
GACCCAATATTAAGATTGCA+TGG - chr3.4:72233767-72233786 None:intergenic 35.0%
GAGGATGATATTTCCATTAG+TGG + chr3.4:72232467-72232486 MS.gene29024:CDS 35.0%
GATTGAAGATCAGGATAAAG+AGG + chr3.4:72231362-72231381 MS.gene29024:CDS 35.0%
GCAAACTTGTATAATCAACG+CGG - chr3.4:72233497-72233516 None:intergenic 35.0%
GCTCCAAAGAGTTGAAATAT+TGG + chr3.4:72233683-72233702 MS.gene29024:CDS 35.0%
GGAAAACAAAACTGATCAGA+GGG + chr3.4:72232103-72232122 MS.gene29024:CDS 35.0%
GGATGATATTTCCATTAGTG+GGG + chr3.4:72232469-72232488 MS.gene29024:CDS 35.0%
TCAATATTTGGAAAGGCTAG+AGG + chr3.4:72232622-72232641 MS.gene29024:CDS 35.0%
TCCAGTTTACTGTCAAACTT+AGG - chr3.4:72231417-72231436 None:intergenic 35.0%
TCCCTTTCAGCTATTCAAAA+TGG + chr3.4:72233858-72233877 MS.gene29024:CDS 35.0%
TCTCAGAATGTTGTTCATGA+TGG + chr3.4:72231390-72231409 MS.gene29024:CDS 35.0%
TGGAAAACAAAACTGATCAG+AGG + chr3.4:72232102-72232121 MS.gene29024:CDS 35.0%
TGTAACCTTCAATTCAGAAG+AGG - chr3.4:72234687-72234706 None:intergenic 35.0%
TTAATATTGGGTCATCCAGA+TGG + chr3.4:72233774-72233793 MS.gene29024:CDS 35.0%
TTGTGCTCGAAAGAATCTAT+GGG - chr3.4:72233073-72233092 None:intergenic 35.0%
! GCCTATGATTCATTTTGCTT+TGG + chr3.4:72234097-72234116 MS.gene29024:CDS 35.0%
! GGAGGCCAACATAAATTTTA+AGG + chr3.4:72231599-72231618 MS.gene29024:CDS 35.0%
! TACATATCAGCCTTATGACT+GGG + chr3.4:72234703-72234722 MS.gene29024:CDS 35.0%
! TTAATTTTGTCTGGTGGTAC+AGG + chr3.4:72233134-72233153 MS.gene29024:intron 35.0%
! TTACATATCAGCCTTATGAC+TGG + chr3.4:72234702-72234721 MS.gene29024:CDS 35.0%
!! CAATCATGTGTATCGCTTTT+TGG + chr3.4:72233326-72233345 MS.gene29024:CDS 35.0%
!!! CAGGTTTACCAACTGTTTTT+TGG + chr3.4:72232568-72232587 MS.gene29024:CDS 35.0%
!!! CTTCATCACTTTTTCCAGAA+AGG - chr3.4:72231938-72231957 None:intergenic 35.0%
AAAAAGTGATGAAGCTGCTG+TGG + chr3.4:72231944-72231963 MS.gene29024:CDS 40.0%
AAAAGTGATGAAGCTGCTGT+GGG + chr3.4:72231945-72231964 MS.gene29024:CDS 40.0%
AAACAACTTGGGAACAGAAG+TGG - chr3.4:72232315-72232334 None:intergenic 40.0%
AATTTGTGGAATCGCTTGTG+TGG - chr3.4:72232696-72232715 None:intergenic 40.0%
ACAGATTGTACAAAATGCGC+AGG + chr3.4:72232549-72232568 MS.gene29024:CDS 40.0%
ACATCTACCCGTAAATTGGA+TGG + chr3.4:72231816-72231835 MS.gene29024:CDS 40.0%
AGACATGGAATCCACAGATA+TGG + chr3.4:72231701-72231720 MS.gene29024:CDS 40.0%
AGATTCTTGACACCCTAGTT+TGG - chr3.4:72232189-72232208 None:intergenic 40.0%
ATCCACAGATATGGATCAGA+AGG + chr3.4:72231710-72231729 MS.gene29024:CDS 40.0%
ATCCTTCCTTTCATGGCTAT+TGG - chr3.4:72232155-72232174 None:intergenic 40.0%
CAAAATGAATCATAGGCTCG+GGG - chr3.4:72234094-72234113 None:intergenic 40.0%
CAACAAATGCTGATCTTCCA+TGG - chr3.4:72231280-72231299 None:intergenic 40.0%
CAGAGGGATTCAGTAACAAA+TGG + chr3.4:72232119-72232138 MS.gene29024:CDS 40.0%
CATGGAAGATCAGCATTTGT+TGG + chr3.4:72231278-72231297 MS.gene29024:CDS 40.0%
CCCTAAGTTTGACAGTAAAC+TGG + chr3.4:72231413-72231432 MS.gene29024:CDS 40.0%
CCTGATCTCCATCCAATTTA+CGG - chr3.4:72231827-72231846 None:intergenic 40.0%
CTATCGCAGAAATTGCATCA+AGG + chr3.4:72233418-72233437 MS.gene29024:CDS 40.0%
CTGATCTCCATCCAATTTAC+GGG - chr3.4:72231826-72231845 None:intergenic 40.0%
CTTGATGCAATTTCTGCGAT+AGG - chr3.4:72233420-72233439 None:intergenic 40.0%
GAAGCCTATGCTATTGAAGA+TGG + chr3.4:72233468-72233487 MS.gene29024:CDS 40.0%
GCAAAATGAATCATAGGCTC+GGG - chr3.4:72234095-72234114 None:intergenic 40.0%
GCATCCATCTTCAATAGCAT+AGG - chr3.4:72233475-72233494 None:intergenic 40.0%
GGCTTCTTAAGGTTATATGG+AGG - chr3.4:72233906-72233925 None:intergenic 40.0%
TAAGGTTATATGGAGGTCGT+TGG - chr3.4:72233899-72233918 None:intergenic 40.0%
TACAAGTTTGCATGGAAGTG+AGG + chr3.4:72233506-72233525 MS.gene29024:CDS 40.0%
TAGTTCAAACCCCAGTCATA+AGG - chr3.4:72234716-72234735 None:intergenic 40.0%
TCATCACCAATAGCCATGAA+AGG + chr3.4:72232146-72232165 MS.gene29024:CDS 40.0%
TCCAAATTGTTGTCCTCACT+TGG - chr3.4:72231747-72231766 None:intergenic 40.0%
TCCAAGTGAGGACAACAATT+TGG + chr3.4:72231743-72231762 MS.gene29024:CDS 40.0%
TGAAATGCACAGATATGGAC+TGG + chr3.4:72231647-72231666 MS.gene29024:CDS 40.0%
TGATACATCGGACGTGATAA+CGG + chr3.4:72231437-72231456 MS.gene29024:CDS 40.0%
TGCCATTCAGATTGAAGATC+AGG + chr3.4:72231353-72231372 MS.gene29024:CDS 40.0%
TGTATACCCTGGTAGAAAGA+CGG + chr3.4:72232268-72232287 MS.gene29024:CDS 40.0%
TTCAATGTCACTGGAGATTC+TGG + chr3.4:72231507-72231526 MS.gene29024:CDS 40.0%
TTGAATAGCTGAAAGGGAGT+AGG - chr3.4:72233856-72233875 None:intergenic 40.0%
TTGGCTAAAGCAGAGAACAA+CGG + chr3.4:72231675-72231694 MS.gene29024:CDS 40.0%
! ACATATCAGCCTTATGACTG+GGG + chr3.4:72234704-72234723 MS.gene29024:CDS 40.0%
! AGAGTATTCTGGTGGTGATA+AGG + chr3.4:72231575-72231594 MS.gene29024:CDS 40.0%
! CGTTTATCTTTTGCACCGAA+TGG - chr3.4:72233927-72233946 None:intergenic 40.0%
! TAACGGATGACAATGATGGA+AGG + chr3.4:72231454-72231473 MS.gene29024:CDS 40.0%
! TCAGCATTTGTTGGAGACAA+AGG + chr3.4:72231287-72231306 MS.gene29024:CDS 40.0%
! TGTAAAGGAGAAAAGTGCAG+TGG + chr3.4:72231890-72231909 MS.gene29024:CDS 40.0%
!! AGGTCTCGTGATTGATTTGA+CGG + chr3.4:72234217-72234236 MS.gene29024:CDS 40.0%
!! CAGATATGGACTGGATGATT+TGG + chr3.4:72231656-72231675 MS.gene29024:CDS 40.0%
!! GGTGATGGGGTTTCAAATAA+CGG - chr3.4:72232434-72232453 None:intergenic 40.0%
!! GTGATAACGGATGACAATGA+TGG + chr3.4:72231450-72231469 MS.gene29024:CDS 40.0%
!! GTTGGTAAAGAGTATTCTGG+TGG + chr3.4:72231567-72231586 MS.gene29024:CDS 40.0%
!! TGCGATAGGCTTTGGATTAA+TGG - chr3.4:72233406-72233425 None:intergenic 40.0%
!! TTCATGGCTATTGGTGATGA+TGG - chr3.4:72232146-72232165 None:intergenic 40.0%
!!! CATCACTTTTTCCAGAAAGG+CGG - chr3.4:72231935-72231954 None:intergenic 40.0%
!!! GTGGGTGATTTTGATGATGA+AGG + chr3.4:72231963-72231982 MS.gene29024:CDS 40.0%
AAAGGAAGCAAGTTCCCAAG+CGG + chr3.4:72231305-72231324 MS.gene29024:CDS 45.0%
AATAGCTGAAAGGGAGTAGG+TGG - chr3.4:72233853-72233872 None:intergenic 45.0%
ACCTCGGTTCCATCAAATAC+AGG - chr3.4:72231771-72231790 None:intergenic 45.0%
ACGGAGTTGGAGAAGATATC+TGG + chr3.4:72232287-72232306 MS.gene29024:CDS 45.0%
ACTCGCAATTTCCTGAGAAG+TGG + chr3.4:72234194-72234213 MS.gene29024:CDS 45.0%
AGAGAACAACGGTAGAGACA+TGG + chr3.4:72231686-72231705 MS.gene29024:CDS 45.0%
AGGTTGCTTACTCGTTGTGT+CGG - chr3.4:72234337-72234356 None:intergenic 45.0%
ATAGGCTTCGAACATTGCAG+AGG - chr3.4:72233457-72233476 None:intergenic 45.0%
ATTTACGGGTAGATGTGCTC+CGG - chr3.4:72231812-72231831 None:intergenic 45.0%
ATTTGATGGAACCGAGGTTC+CGG + chr3.4:72231773-72231792 MS.gene29024:CDS 45.0%
CACCAATAGCCATGAAAGGA+AGG + chr3.4:72232150-72232169 MS.gene29024:CDS 45.0%
CCGTAAATTGGATGGAGATC+AGG + chr3.4:72231824-72231843 MS.gene29024:CDS 45.0%
CCTCGGTTCCATCAAATACA+GGG - chr3.4:72231770-72231789 None:intergenic 45.0%
CTCCAGAAAAAGGTGGTGAT+GGG - chr3.4:72232448-72232467 None:intergenic 45.0%
CTTCTTTCCAATGCTCCATC+TGG - chr3.4:72233792-72233811 None:intergenic 45.0%
GAGCACATCTACCCGTAAAT+TGG + chr3.4:72231812-72231831 MS.gene29024:CDS 45.0%
GCAATTTCTGCGATAGGCTT+TGG - chr3.4:72233414-72233433 None:intergenic 45.0%
GCTTGCCTCTTCTGAATTGA+AGG + chr3.4:72234679-72234698 MS.gene29024:CDS 45.0%
GGCAGATGAAATTCCAAGTG+AGG + chr3.4:72231731-72231750 MS.gene29024:CDS 45.0%
GTCTGAGCCCAAAAAACAGT+TGG - chr3.4:72232579-72232598 None:intergenic 45.0%
TCAATTCAGAAGAGGCAAGC+AGG - chr3.4:72234679-72234698 None:intergenic 45.0%
TCATCCTCTCCAGAAAAAGG+TGG - chr3.4:72232455-72232474 None:intergenic 45.0%
TCTCCAGAAAAAGGTGGTGA+TGG - chr3.4:72232449-72232468 None:intergenic 45.0%
TGCATCAAGGCTGAGATTTC+TGG + chr3.4:72233431-72233450 MS.gene29024:CDS 45.0%
TGCCTTCTGATCCATATCTG+TGG - chr3.4:72231715-72231734 None:intergenic 45.0%
TGGCTTCTCAGAAACCAGTT+TGG - chr3.4:72232024-72232043 None:intergenic 45.0%
TTCATCAACTGCCCCACTAA+TGG - chr3.4:72232483-72232502 None:intergenic 45.0%
TTCCAAGCAGATTTCTCCAC+TGG - chr3.4:72232044-72232063 None:intergenic 45.0%
TTGCACCGAATGGCTTCTTA+AGG - chr3.4:72233917-72233936 None:intergenic 45.0%
TTGCATGGAAGTGAGGAGTT+CGG + chr3.4:72233513-72233532 MS.gene29024:CDS 45.0%
! GTATTCTGGTGGTGATAAGG+TGG + chr3.4:72231578-72231597 MS.gene29024:CDS 45.0%
! TCCAGAAAAAGGTGGTGATG+GGG - chr3.4:72232447-72232466 None:intergenic 45.0%
! TCCAGGAGAATAGCATCGAA+GGG - chr3.4:72234147-72234166 None:intergenic 45.0%
! TTCCAGGAGAATAGCATCGA+AGG - chr3.4:72234148-72234167 None:intergenic 45.0%
! TTTTGGCAAAAGCGAGCTTG+AGG + chr3.4:72234598-72234617 MS.gene29024:CDS 45.0%
!! ATCACCACCTTTTTCTGGAG+AGG + chr3.4:72232448-72232467 MS.gene29024:CDS 45.0%
!! CAGATGGAGCATTGGAAAGA+AGG + chr3.4:72233790-72233809 MS.gene29024:CDS 45.0%
!! TAGTGGGGCAGTTGATGAAT+TGG + chr3.4:72232484-72232503 MS.gene29024:CDS 45.0%
AGCCAGTGGAGAAATCTGCT+TGG + chr3.4:72232039-72232058 MS.gene29024:CDS 50.0%
CCAACTCCGTCTTTCTACCA+GGG - chr3.4:72232277-72232296 None:intergenic 50.0%
CCCTGTATTTGATGGAACCG+AGG + chr3.4:72231767-72231786 MS.gene29024:CDS 50.0%
CGCAATTTCCTGAGAAGTGG+AGG + chr3.4:72234197-72234216 MS.gene29024:CDS 50.0%
GAATCATAGGCTCGGGGTAA+GGG - chr3.4:72234088-72234107 None:intergenic 50.0%
TCCAACTCCGTCTTTCTACC+AGG - chr3.4:72232278-72232297 None:intergenic 50.0%
TGAATCATAGGCTCGGGGTA+AGG - chr3.4:72234089-72234108 None:intergenic 50.0%
TTCCGGGGATGCAAGTTAAC+CGG + chr3.4:72231790-72231809 MS.gene29024:CDS 50.0%
TTGCAGCTTCTCAACCGCTT+GGG - chr3.4:72231322-72231341 None:intergenic 50.0%
TTTGATGGAACCGAGGTTCC+GGG + chr3.4:72231774-72231793 MS.gene29024:CDS 50.0%
! ACCCCATCACCACCTTTTTC+TGG + chr3.4:72232443-72232462 MS.gene29024:CDS 50.0%
! ACTGGTTTCTGAGAAGCCAG+TGG + chr3.4:72232025-72232044 MS.gene29024:CDS 50.0%
! TTCTGGTGGTGATAAGGTGG+AGG + chr3.4:72231581-72231600 MS.gene29024:CDS 50.0%
AACTTGCATCCCCGGAACCT+CGG - chr3.4:72231787-72231806 None:intergenic 55.0%
ACTGCCTTCTCAACCACACC+TGG - chr3.4:72231855-72231874 None:intergenic 55.0%
AGATCAGGACAGTCCAGGTG+TGG + chr3.4:72231839-72231858 MS.gene29024:CDS 55.0%
ATGGAAGTGAGGAGTTCGGC+AGG + chr3.4:72233517-72233536 MS.gene29024:CDS 55.0%
CAGTCCAGGTGTGGTTGAGA+AGG + chr3.4:72231848-72231867 MS.gene29024:CDS 55.0%
CCCTGGTAGAAAGACGGAGT+TGG + chr3.4:72232274-72232293 MS.gene29024:CDS 55.0%
CTTGCAGCTTCTCAACCGCT+TGG - chr3.4:72231323-72231342 None:intergenic 55.0%
GATGGAGATCAGGACAGTCC+AGG + chr3.4:72231834-72231853 MS.gene29024:CDS 55.0%
GCCCTTCGATGCTATTCTCC+TGG + chr3.4:72234143-72234162 MS.gene29024:CDS 55.0%
GGGTCATCCAGATGGAGCAT+TGG + chr3.4:72233782-72233801 MS.gene29024:CDS 55.0%
TCACGAGACCTCCACTTCTC+AGG - chr3.4:72234208-72234227 None:intergenic 55.0%
TGGTATGTGGCACCCGATCT+GGG + chr3.4:72234117-72234136 MS.gene29024:CDS 55.0%
TTGATGGAACCGAGGTTCCG+GGG + chr3.4:72231775-72231794 MS.gene29024:CDS 55.0%
TTGGTATGTGGCACCCGATC+TGG + chr3.4:72234116-72234135 MS.gene29024:CDS 55.0%
! GGAGAATAGCATCGAAGGGC+AGG - chr3.4:72234143-72234162 None:intergenic 55.0%
!! CTCCGGTTAACTTGCATCCC+CGG - chr3.4:72231795-72231814 None:intergenic 55.0%
ACTATGCTGCGCCGCCTTTC+TGG + chr3.4:72231921-72231940 MS.gene29024:CDS 60.0%
AGAAAGGCGGCGCAGCATAG+TGG - chr3.4:72231922-72231941 None:intergenic 60.0%
ATCGAAGGGCAGGCCCAGAT+CGG - chr3.4:72234133-72234152 None:intergenic 60.0%
TCGAAGGGCAGGCCCAGATC+GGG - chr3.4:72234132-72234151 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 72231267 72234739 72231267 ID=MS.gene29024
chr3.4 mRNA 72231267 72234739 72231267 ID=MS.gene29024.t1;Parent=MS.gene29024
chr3.4 exon 72231267 72232643 72231267 ID=MS.gene29024.t1.exon1;Parent=MS.gene29024.t1
chr3.4 CDS 72231267 72232643 72231267 ID=cds.MS.gene29024.t1;Parent=MS.gene29024.t1
chr3.4 exon 72233156 72233211 72233156 ID=MS.gene29024.t1.exon2;Parent=MS.gene29024.t1
chr3.4 CDS 72233156 72233211 72233156 ID=cds.MS.gene29024.t1;Parent=MS.gene29024.t1
chr3.4 exon 72233305 72233538 72233305 ID=MS.gene29024.t1.exon3;Parent=MS.gene29024.t1
chr3.4 CDS 72233305 72233538 72233305 ID=cds.MS.gene29024.t1;Parent=MS.gene29024.t1
chr3.4 exon 72233662 72233953 72233662 ID=MS.gene29024.t1.exon4;Parent=MS.gene29024.t1
chr3.4 CDS 72233662 72233953 72233662 ID=cds.MS.gene29024.t1;Parent=MS.gene29024.t1
chr3.4 exon 72234077 72234273 72234077 ID=MS.gene29024.t1.exon5;Parent=MS.gene29024.t1
chr3.4 CDS 72234077 72234273 72234077 ID=cds.MS.gene29024.t1;Parent=MS.gene29024.t1
chr3.4 exon 72234586 72234739 72234586 ID=MS.gene29024.t1.exon6;Parent=MS.gene29024.t1
chr3.4 CDS 72234586 72234739 72234586 ID=cds.MS.gene29024.t1;Parent=MS.gene29024.t1
Gene Sequence

>MS.gene29024

CTTGCTATCGCCATGGAAGATCAGCATTTGTTGGAGACAAAGGAAGCAAGTTCCCAAGCGGTTGAGAAGCTGCAAGTAGATGCTTCTGCCATTCAGATTGAAGATCAGGATAAAGAGGCAAAATCTCAGAATGTTGTTCATGATGGCCCTAAGTTTGACAGTAAACTGGATGATACATCGGACGTGATAACGGATGACAATGATGGAAGGTCTAAGTCTGATACTGATGAAGAAAAAAAATTCAATGTCACTGGAGATTCTGGTTTAGCTCCGATCAATTCTGAAACTGAAATTGATAATGTTGGTAAAGAGTATTCTGGTGGTGATAAGGTGGAGGCCAACATAAATTTTAAGGCTAAAAGTGAAGAGACAAATCTAGATGAAATGCACAGATATGGACTGGATGATTTGGCTAAAGCAGAGAACAACGGTAGAGACATGGAATCCACAGATATGGATCAGAAGGCAGATGAAATTCCAAGTGAGGACAACAATTTGGACCCTGTATTTGATGGAACCGAGGTTCCGGGGATGCAAGTTAACCGGAGCACATCTACCCGTAAATTGGATGGAGATCAGGACAGTCCAGGTGTGGTTGAGAAGGCAGTCGCTCTTAAAAATCTTGTAAAGGAGAAAAGTGCAGTGGCAGTCTCCACTATGCTGCGCCGCCTTTCTGGAAAAAGTGATGAAGCTGCTGTGGGTGATTTTGATGATGAAGGCAATAATGTTTCAGATGTCTCAGAAGTTAATGAAGCCAAACTGGTTTCTGAGAAGCCAGTGGAGAAATCTGCTTGGAATCCCTTAAATTATATTAAGAAGTCATTTGATGTTGATGTGGAAAACAAAACTGATCAGAGGGATTCAGTAACAAATGGTCCATCATCACCAATAGCCATGAAAGGAAGGATTATACTGTACACCAAACTAGGGTGTCAAGAATCTAGAATATTTAGGCTATTTTTACGTACAAAAAGACTTAGGTTTGTGGAAATCAACATAGATGTATACCCTGGTAGAAAGACGGAGTTGGAGAAGATATCTGGATCCACTTCTGTTCCCAAGTTGTTTTTCAATGAAATATGTATTGGAGGCTTGAGTGAGCTTAAAAACTTGGATGAATCTGGAAAGCTTGATGAGAAGATTGACTTTCTTATTACTGAAGCACCGTTATTTGAAACCCCATCACCACCTTTTTCTGGAGAGGATGATATTTCCATTAGTGGGGCAGTTGATGAATTGGCTCTTGTTGTACACAAAATGAAAGAATCTATTACTGTGAAAGACAGATTGTACAAAATGCGCAGGTTTACCAACTGTTTTTTGGGCTCAGACGCTTTAGACTTCTTATCAGAAGATCAATATTTGGAAAGGCTAGAGGTAAGTTTGTCTAATTGTCTTAGCTTTGGGTCTAATGTGTCAATAAACACCACACAAGCGATTCCACAAATTCATACGTAATCAAACAAATCATACACTAAATATCACATATATTTACTTACATACAGTTTCATGATCAAACAAATCATACACTAAATTTCTCATAGATTCAGAAGATAATATATAATGTATTACATAAAATGAGGTCTAACTATACTTTGAATGACATAATCAAACAAATCATATCACACTTATCACATAGATTCTTTCAAATACAATTTGTAGGTCTTATCAAAACAATCAAAAAAATTATACCCTAAATATCCCATAGATTTAAAAGATCATATATAATAGTGTATTGCATAGAACTCTATTTTGTTATGATTTGAATAACACAATCAAACAAACAAATCATACACTCAATATCCCATAGATTCTTTCGAGCACAATTTATTTTTGGCTTCACAAAGCCATTGTTTTTCTTCTTTTTTTAATTTTGTCTGGTGGTACAGGCTGTTGAATTTGCTCGAAAGCTTGCTGACAAATTTTTCTTTCGACATGTTCTTGAGTGAGTCTATATACTTAGAGTTCTGATTTTTAATGTTTAATTTGTTTTGTTGTTCCAAAGTAACAATATTTACTAATTTTTGTTTTGATTCAGTGAAAATTTATTTGAAGATGGCAATCATGTGTATCGCTTTTTGGATGATGATCCAATTGTGATGTCACAATGTTACAATATTCCAAAGGGTATTATTACCATTAATCCAAAGCCTATCGCAGAAATTGCATCAAGGCTGAGATTTCTGGCCTCTGCAATGTTCGAAGCCTATGCTATTGAAGATGGATGCCGCGTTGATTATACAAGTTTGCATGGAAGTGAGGAGTTCGGCAGGTGTCTTCATTATTCTTTGTATTTTGTTTTCCGTTAGAAGTTTAGAACTCTGACGTTTTATACAATCTTTGATAGTCCATTCAGTCATTTTGTAAGTTCACATGCTATTTGTTGACATTGTAGGTATCTGAGAATAGTTGAAGAGCTCCAAAGAGTTGAAATATTGGATTTGTCTAGAGAAGAAAAGCTTGCATTCTTTATTAATCTTTATAACATGATGACCATCCATGCAATCTTAATATTGGGTCATCCAGATGGAGCATTGGAAAGAAGGAAATTATTTGGAGAGTTCAAATATGTTATAGGTGGTTCCACCTACTCCCTTTCAGCTATTCAAAATGGAATATTGAGGGGAAACCAACGACCTCCATATAACCTTAAGAAGCCATTCGGTGCAAAAGATAAACGCTTAAAGGTAAGCTACTTTTGTTGTTGTTGTTGTTTGGCACAATCACTTTCATGTTTAAATTAATTATTTTAGAATCACATGTAGACTTTCATCAGTTTTGATGCTCACGTTGATTCATTTATTTGATAGGTTGCACTCCCTTACCCCGAGCCTATGATTCATTTTGCTTTGGTATGTGGCACCCGATCTGGGCCTGCCCTTCGATGCTATTCTCCTGGAAAAATTGACGAAGAATTATTGGATGCAACTCGCAATTTCCTGAGAAGTGGAGGTCTCGTGATTGATTTGACGGCAAAGACTGCATATGTCAGTAAGATTCTTAAATGGTAAGTTCTTTTAAACAAAGAAAACAACTCCTTAATATCTTATACACTAGCACATTTTATCCGACACAACGAGTAAGCAACCTTTTAAAATTCTATACTTAATTGAATGTTTCAAATTTAAAGTACTGAAGATTTTAATGTGATATGTAAAATTTGTAGAAATGTCATTTAAAATTTCGTTTTGATATTTAATGTGATATGTAAAACTTAAACATGAACAATATTGTTGTTAAATGAAAGCAGTGTTAGAAATTGTGTTTACTTTGGTGATGTTGAAACTGAAAATAACTATTCCTCATTGTTCATATTTAGGTATAGCATAGATTTTGGCAAAAGCGAGCTTGAGGTGATCAAGCATGTGTCAAACTATTTAGATCCAACTGACTCAGCAATATTATTGGACCTGCTTGCCTCTTCTGAATTGAAGGTTACATATCAGCCTTATGACTGGGGTTTGAACTACTAG
Protein sequence

>MS.gene29024.t1

LAIAMEDQHLLETKEASSQAVEKLQVDASAIQIEDQDKEAKSQNVVHDGPKFDSKLDDTSDVITDDNDGRSKSDTDEEKKFNVTGDSGLAPINSETEIDNVGKEYSGGDKVEANINFKAKSEETNLDEMHRYGLDDLAKAENNGRDMESTDMDQKADEIPSEDNNLDPVFDGTEVPGMQVNRSTSTRKLDGDQDSPGVVEKAVALKNLVKEKSAVAVSTMLRRLSGKSDEAAVGDFDDEGNNVSDVSEVNEAKLVSEKPVEKSAWNPLNYIKKSFDVDVENKTDQRDSVTNGPSSPIAMKGRIILYTKLGCQESRIFRLFLRTKRLRFVEINIDVYPGRKTELEKISGSTSVPKLFFNEICIGGLSELKNLDESGKLDEKIDFLITEAPLFETPSPPFSGEDDISISGAVDELALVVHKMKESITVKDRLYKMRRFTNCFLGSDALDFLSEDQYLERLEAVEFARKLADKFFFRHVLDENLFEDGNHVYRFLDDDPIVMSQCYNIPKGIITINPKPIAEIASRLRFLASAMFEAYAIEDGCRVDYTSLHGSEEFGRYLRIVEELQRVEILDLSREEKLAFFINLYNMMTIHAILILGHPDGALERRKLFGEFKYVIGGSTYSLSAIQNGILRGNQRPPYNLKKPFGAKDKRLKVALPYPEPMIHFALVCGTRSGPALRCYSPGKIDEELLDATRNFLRSGGLVIDLTAKTAYVSKILKWYSIDFGKSELEVIKHVSNYLDPTDSAILLDLLASSELKVTYQPYDWGLNY