Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34298.t1 | XP_013454503.1 | 95.8 | 95 | 4 | 0 | 1 | 95 | 118 | 212 | 1.40E-43 | 185.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34298.t1 | Q9SUE0 | 86.2 | 87 | 12 | 0 | 7 | 93 | 127 | 213 | 3.0e-39 | 162.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34298.t1 | A0A072UFK1 | 95.8 | 95 | 4 | 0 | 1 | 95 | 118 | 212 | 9.8e-44 | 185.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene34298 | 0.83203 | 1.19E-55 | -1.69E-46 |
| MS.gene049173 | MS.gene34298 | 0.801656 | 8.12E-49 | -1.69E-46 |
| MS.gene049285 | MS.gene34298 | 0.807843 | 4.15E-50 | -1.69E-46 |
| MS.gene050163 | MS.gene34298 | 0.832022 | 1.20E-55 | -1.69E-46 |
| MS.gene050165 | MS.gene34298 | 0.824572 | 7.51E-54 | -1.69E-46 |
| MS.gene050333 | MS.gene34298 | 0.829564 | 4.80E-55 | -1.69E-46 |
| MS.gene050444 | MS.gene34298 | 0.805937 | 1.05E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene34298 | 0.818892 | 1.55E-52 | -1.69E-46 |
| MS.gene050612 | MS.gene34298 | 0.802352 | 5.84E-49 | -1.69E-46 |
| MS.gene051874 | MS.gene34298 | 0.800428 | 1.45E-48 | -1.69E-46 |
| MS.gene052872 | MS.gene34298 | 0.800935 | 1.14E-48 | -1.69E-46 |
| MS.gene053009 | MS.gene34298 | 0.800646 | 1.31E-48 | -1.69E-46 |
| MS.gene053284 | MS.gene34298 | 0.974825 | 7.66E-139 | -1.69E-46 |
| MS.gene053285 | MS.gene34298 | 0.984665 | 3.17E-161 | -1.69E-46 |
| MS.gene053916 | MS.gene34298 | 0.806634 | 7.48E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene34298 | 0.800343 | 1.51E-48 | -1.69E-46 |
| MS.gene055577 | MS.gene34298 | 0.803561 | 3.29E-49 | -1.69E-46 |
| MS.gene055748 | MS.gene34298 | 0.823788 | 1.15E-53 | -1.69E-46 |
| MS.gene055751 | MS.gene34298 | 0.816309 | 5.91E-52 | -1.69E-46 |
| MS.gene057129 | MS.gene34298 | 0.823627 | 1.25E-53 | -1.69E-46 |
| MS.gene058773 | MS.gene34298 | 0.817662 | 2.94E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene34298 | 0.812483 | 4.14E-51 | -1.69E-46 |
| MS.gene059023 | MS.gene34298 | 0.807327 | 5.34E-50 | -1.69E-46 |
| MS.gene059677 | MS.gene34298 | 0.803125 | 4.05E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene34298 | 0.813044 | 3.12E-51 | -1.69E-46 |
| MS.gene06111 | MS.gene34298 | 0.814059 | 1.87E-51 | -1.69E-46 |
| MS.gene061150 | MS.gene34298 | 0.851359 | 9.34E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34298.t1 | MTR_4g005520 | 95.789 | 95 | 4 | 0 | 1 | 95 | 118 | 212 | 4.30e-62 | 187 |
| MS.gene34298.t1 | MTR_3g105100 | 47.368 | 76 | 35 | 3 | 15 | 89 | 120 | 191 | 2.63e-14 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34298.t1 | AT4G27990 | 85.227 | 88 | 13 | 0 | 7 | 94 | 127 | 214 | 8.55e-52 | 162 |
| MS.gene34298.t1 | AT3G07430 | 83.908 | 87 | 14 | 0 | 7 | 93 | 141 | 227 | 5.25e-51 | 160 |
| MS.gene34298.t1 | AT5G21920 | 50.000 | 76 | 33 | 3 | 15 | 89 | 133 | 204 | 8.24e-15 | 67.4 |
Find 24 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACAACCATTGTCTGCAATT+AGG | 0.283838 | 4.3:+88887099 | MS.gene34298:CDS |
| ACAACCATTGTCTGCAATTA+GGG | 0.307081 | 4.3:+88887100 | MS.gene34298:CDS |
| ACAGTGGTGTGTTGTTGGTT+AGG | 0.327645 | 4.3:+88887039 | MS.gene34298:CDS |
| ATCCTTATCTAAGTTTGTTT+AGG | 0.329465 | 4.3:+88887132 | MS.gene34298:CDS |
| CAGTGGTGTGTTGTTGGTTA+GGG | 0.363509 | 4.3:+88887040 | MS.gene34298:CDS |
| TAGCGGCGGGGTTGAAGAAA+TGG | 0.403666 | 4.3:+88887006 | MS.gene34298:CDS |
| TATTTACAGTGGTGTGTTGT+TGG | 0.416695 | 4.3:+88887034 | MS.gene34298:CDS |
| GACGGTGAACACGCCGTTGA+CGG | 0.439588 | 4.3:+88886980 | MS.gene34298:CDS |
| CCGTTGACGGTTGTAGCGGC+GGG | 0.441420 | 4.3:+88886993 | MS.gene34298:CDS |
| TGTCTTTCCCATGGAATGTT+GGG | 0.454889 | 4.3:-88887080 | None:intergenic |
| GTTGGTTTCCCAACATTCCA+TGG | 0.512936 | 4.3:+88887072 | MS.gene34298:CDS |
| GACAATGGTTGTCTTTCCCA+TGG | 0.515980 | 4.3:-88887089 | None:intergenic |
| TTCCTAAACAAACTTAGATA+AGG | 0.528049 | 4.3:-88887134 | None:intergenic |
| GCCGTTGACGGTTGTAGCGG+CGG | 0.535668 | 4.3:+88886992 | MS.gene34298:CDS |
| TTGGTTTCCCAACATTCCAT+GGG | 0.548737 | 4.3:+88887073 | MS.gene34298:CDS |
| CCCGCCGCTACAACCGTCAA+CGG | 0.573983 | 4.3:-88886993 | None:intergenic |
| TTGTCTTTCCCATGGAATGT+TGG | 0.574451 | 4.3:-88887081 | None:intergenic |
| AAAACAGCAAAAGCAAGAAG+AGG | 0.574512 | 4.3:-88887194 | None:intergenic |
| CACGCCGTTGACGGTTGTAG+CGG | 0.575214 | 4.3:+88886989 | MS.gene34298:CDS |
| CGTTGACGGTTGTAGCGGCG+GGG | 0.610796 | 4.3:+88886994 | MS.gene34298:CDS |
| GGCGGGGTTGAAGAAATGGT+TGG | 0.623671 | 4.3:+88887010 | MS.gene34298:CDS |
| AAATGGTTGGATATTTACAG+TGG | 0.642279 | 4.3:+88887023 | MS.gene34298:CDS |
| AGATCCCTAATTGCAGACAA+TGG | 0.662621 | 4.3:-88887104 | None:intergenic |
| GGATCTAAACAGGAACAGTG+AGG | 0.722011 | 4.3:-88887235 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTCCTAAACAAACTTAGATA+AGG | - | chr4.3:88887137-88887156 | None:intergenic | 25.0% |
| !! | ATCCTTATCTAAGTTTGTTT+AGG | + | chr4.3:88887132-88887151 | MS.gene34298:CDS | 25.0% |
| !!! | ATATCAAGAGTATCAAAAAC+AGG | - | chr4.3:88887170-88887189 | None:intergenic | 25.0% |
| AAATGGTTGGATATTTACAG+TGG | + | chr4.3:88887023-88887042 | MS.gene34298:CDS | 30.0% | |
| AAAACAGCAAAAGCAAGAAG+AGG | - | chr4.3:88887197-88887216 | None:intergenic | 35.0% | |
| ACAACCATTGTCTGCAATTA+GGG | + | chr4.3:88887100-88887119 | MS.gene34298:CDS | 35.0% | |
| TATTTACAGTGGTGTGTTGT+TGG | + | chr4.3:88887034-88887053 | MS.gene34298:CDS | 35.0% | |
| !! | TCAAGAGTATCAAAAACAGG+TGG | - | chr4.3:88887167-88887186 | None:intergenic | 35.0% |
| !!! | CTTCTTGCTTTTGCTGTTTT+GGG | + | chr4.3:88887197-88887216 | MS.gene34298:CDS | 35.0% |
| !!! | TCTTCTTGCTTTTGCTGTTT+TGG | + | chr4.3:88887196-88887215 | MS.gene34298:CDS | 35.0% |
| AGATCCCTAATTGCAGACAA+TGG | - | chr4.3:88887107-88887126 | None:intergenic | 40.0% | |
| GACAACCATTGTCTGCAATT+AGG | + | chr4.3:88887099-88887118 | MS.gene34298:CDS | 40.0% | |
| TGTCTTTCCCATGGAATGTT+GGG | - | chr4.3:88887083-88887102 | None:intergenic | 40.0% | |
| TTGGTTTCCCAACATTCCAT+GGG | + | chr4.3:88887073-88887092 | MS.gene34298:CDS | 40.0% | |
| TTGTCTTTCCCATGGAATGT+TGG | - | chr4.3:88887084-88887103 | None:intergenic | 40.0% | |
| !!! | TGGTTAGGGTTTTGTTGAGT+TGG | + | chr4.3:88887054-88887073 | MS.gene34298:CDS | 40.0% |
| GACAATGGTTGTCTTTCCCA+TGG | - | chr4.3:88887092-88887111 | None:intergenic | 45.0% | |
| ! | ACAGTGGTGTGTTGTTGGTT+AGG | + | chr4.3:88887039-88887058 | MS.gene34298:CDS | 45.0% |
| ! | CAGTGGTGTGTTGTTGGTTA+GGG | + | chr4.3:88887040-88887059 | MS.gene34298:CDS | 45.0% |
| ! | GTTGGTTTCCCAACATTCCA+TGG | + | chr4.3:88887072-88887091 | MS.gene34298:CDS | 45.0% |
| !! | CCCGTCCTAGTCTTTTTCAT+AGG | - | chr4.3:88886960-88886979 | None:intergenic | 45.0% |
| GAAAAAGACTAGGACGGGGA+CGG | + | chr4.3:88886962-88886981 | MS.gene34298:CDS | 50.0% | |
| !! | GCTGTTTTGGGTACTCTTGC+TGG | + | chr4.3:88887209-88887228 | MS.gene34298:CDS | 50.0% |
| GGCGGGGTTGAAGAAATGGT+TGG | + | chr4.3:88887010-88887029 | MS.gene34298:CDS | 55.0% | |
| TAGCGGCGGGGTTGAAGAAA+TGG | + | chr4.3:88887006-88887025 | MS.gene34298:CDS | 55.0% | |
| CACGCCGTTGACGGTTGTAG+CGG | + | chr4.3:88886989-88887008 | MS.gene34298:CDS | 60.0% | |
| GACGGTGAACACGCCGTTGA+CGG | + | chr4.3:88886980-88886999 | MS.gene34298:CDS | 60.0% | |
| CCCGCCGCTACAACCGTCAA+CGG | - | chr4.3:88886996-88887015 | None:intergenic | 65.0% | |
| CCGTTGACGGTTGTAGCGGC+GGG | + | chr4.3:88886993-88887012 | MS.gene34298:CDS | 65.0% | |
| CGTTGACGGTTGTAGCGGCG+GGG | + | chr4.3:88886994-88887013 | MS.gene34298:CDS | 65.0% | |
| GCCGTTGACGGTTGTAGCGG+CGG | + | chr4.3:88886992-88887011 | MS.gene34298:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 88886960 | 88887247 | 88886960 | ID=MS.gene34298 |
| chr4.3 | mRNA | 88886960 | 88887247 | 88886960 | ID=MS.gene34298.t1;Parent=MS.gene34298 |
| chr4.3 | exon | 88886960 | 88887247 | 88886960 | ID=MS.gene34298.t1.exon1;Parent=MS.gene34298.t1 |
| chr4.3 | CDS | 88886960 | 88887247 | 88886960 | ID=cds.MS.gene34298.t1;Parent=MS.gene34298.t1 |
| Gene Sequence |
| Protein sequence |
