Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34317.t1 | XP_013454489.1 | 100 | 69 | 0 | 0 | 1 | 69 | 73 | 141 | 7.50E-28 | 132.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34317.t1 | Q8GWH5 | 81.2 | 69 | 13 | 0 | 1 | 69 | 58 | 126 | 3.3e-24 | 111.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34317.t1 | A0A072UFI9 | 100.0 | 69 | 0 | 0 | 1 | 69 | 73 | 141 | 5.4e-28 | 132.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049322 | MS.gene34317 | -0.813032 | 3.14E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene34317 | -0.807256 | 5.52E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene34317 | -0.801375 | 9.28E-49 | -1.69E-46 |
| MS.gene050317 | MS.gene34317 | -0.80049 | 1.41E-48 | -1.69E-46 |
| MS.gene050623 | MS.gene34317 | 0.834948 | 2.23E-56 | -1.69E-46 |
| MS.gene050762 | MS.gene34317 | -0.840235 | 9.82E-58 | -1.69E-46 |
| MS.gene050763 | MS.gene34317 | -0.840666 | 7.58E-58 | -1.69E-46 |
| MS.gene051245 | MS.gene34317 | -0.803377 | 3.59E-49 | -1.69E-46 |
| MS.gene052302 | MS.gene34317 | -0.80158 | 8.42E-49 | -1.69E-46 |
| MS.gene052856 | MS.gene34317 | -0.800154 | 1.65E-48 | -1.69E-46 |
| MS.gene052970 | MS.gene34317 | 0.947564 | 5.27E-106 | -1.69E-46 |
| MS.gene054379 | MS.gene34317 | -0.828407 | 9.15E-55 | -1.69E-46 |
| MS.gene055413 | MS.gene34317 | -0.820035 | 8.48E-53 | -1.69E-46 |
| MS.gene055441 | MS.gene34317 | -0.804227 | 2.39E-49 | -1.69E-46 |
| MS.gene056552 | MS.gene34317 | -0.809207 | 2.12E-50 | -1.69E-46 |
| MS.gene057057 | MS.gene34317 | -0.812383 | 4.36E-51 | -1.69E-46 |
| MS.gene057452 | MS.gene34317 | 0.807427 | 5.08E-50 | -1.69E-46 |
| MS.gene058182 | MS.gene34317 | -0.821012 | 5.06E-53 | -1.69E-46 |
| MS.gene060084 | MS.gene34317 | -0.848442 | 6.11E-60 | -1.69E-46 |
| MS.gene060297 | MS.gene34317 | -0.823448 | 1.38E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTCTTGGAAGACTGGCTT+TGG | 0.289145 | 4.3:+89193636 | MS.gene34317:CDS |
| CTTTATGCATTATCTGCTCT+TGG | 0.374994 | 4.3:+89193622 | MS.gene34317:CDS |
| GTCTTGAGGGTTGCTGCTAC+TGG | 0.407570 | 4.3:+89193272 | MS.gene34317:CDS |
| GCTCTTGGAAGACTGGCTTT+GGG | 0.418794 | 4.3:+89193637 | MS.gene34317:CDS |
| TGCCACTCAAGGGGATCAAC+AGG | 0.434496 | 4.3:-89193182 | None:intergenic |
| TGCCGCTGAATCTGTCTTGA+GGG | 0.443932 | 4.3:+89193259 | MS.gene34317:CDS |
| CAAGAGCAGATAATGCATAA+AGG | 0.448013 | 4.3:-89193621 | None:intergenic |
| ATTATCTGCTCTTGGAAGAC+TGG | 0.464565 | 4.3:+89193630 | MS.gene34317:CDS |
| GGTATTACTATCGCCTATGC+TGG | 0.488512 | 4.3:+89193658 | MS.gene34317:CDS |
| GCTTCTTTCGCTAATACTGT+CGG | 0.494988 | 4.3:+89193236 | MS.gene34317:CDS |
| TTGAGATATTTGCCACTCAA+GGG | 0.504422 | 4.3:-89193192 | None:intergenic |
| CTTGAGATATTTGCCACTCA+AGG | 0.517790 | 4.3:-89193193 | None:intergenic |
| GTGCCGCTGAATCTGTCTTG+AGG | 0.538110 | 4.3:+89193258 | MS.gene34317:CDS |
| AGAAAGGTACGAACCAGCAT+AGG | 0.589035 | 4.3:-89193671 | None:intergenic |
| TGAGATATTTGCCACTCAAG+GGG | 0.608929 | 4.3:-89193191 | None:intergenic |
| TTCCTGTTGATCCCCTTGAG+TGG | 0.613108 | 4.3:+89193180 | MS.gene34317:CDS |
| AACCCTCAAGACAGATTCAG+CGG | 0.632761 | 4.3:-89193261 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAAGTTAGAAATATAAAAA+AGG | - | chr4.3:89193567-89193586 | None:intergenic | 15.0% |
| !!! | TGACAAAAGACTCTTTTTTA+AGG | + | chr4.3:89193298-89193317 | MS.gene34317:CDS | 25.0% |
| AAAGAATGACCTGTAATCAT+TGG | - | chr4.3:89193600-89193619 | None:intergenic | 30.0% | |
| TGAAAAACTGAAATAGACAC+CGG | + | chr4.3:89193362-89193381 | MS.gene34317:intron | 30.0% | |
| ACATCTTCAATCTCAAGTGT+CGG | + | chr4.3:89193485-89193504 | MS.gene34317:intron | 35.0% | |
| ACTCATCTACCAATGATTAC+AGG | + | chr4.3:89193588-89193607 | MS.gene34317:intron | 35.0% | |
| CAAGAGCAGATAATGCATAA+AGG | - | chr4.3:89193624-89193643 | None:intergenic | 35.0% | |
| CTTTATGCATTATCTGCTCT+TGG | + | chr4.3:89193622-89193641 | MS.gene34317:CDS | 35.0% | |
| TTGAGATATTTGCCACTCAA+GGG | - | chr4.3:89193195-89193214 | None:intergenic | 35.0% | |
| ATTATCTGCTCTTGGAAGAC+TGG | + | chr4.3:89193630-89193649 | MS.gene34317:CDS | 40.0% | |
| CTTGAGATATTTGCCACTCA+AGG | - | chr4.3:89193196-89193215 | None:intergenic | 40.0% | |
| GCTTCTTTCGCTAATACTGT+CGG | + | chr4.3:89193236-89193255 | MS.gene34317:CDS | 40.0% | |
| TGAGATATTTGCCACTCAAG+GGG | - | chr4.3:89193194-89193213 | None:intergenic | 40.0% | |
| AACCCTCAAGACAGATTCAG+CGG | - | chr4.3:89193264-89193283 | None:intergenic | 45.0% | |
| ACACTTGGGTTTCGTAAGTG+TGG | + | chr4.3:89193527-89193546 | MS.gene34317:intron | 45.0% | |
| ACTGAAATAGACACCGGACA+TGG | + | chr4.3:89193368-89193387 | MS.gene34317:intron | 45.0% | |
| GTTATGTTCGGCTGACACTT+GGG | + | chr4.3:89193513-89193532 | MS.gene34317:intron | 45.0% | |
| TGTTATGTTCGGCTGACACT+TGG | + | chr4.3:89193512-89193531 | MS.gene34317:intron | 45.0% | |
| ! | GTGTCGGTCAGTGTTATGTT+CGG | + | chr4.3:89193501-89193520 | MS.gene34317:intron | 45.0% |
| TGAGTCGTTGTGTCCATGTC+CGG | - | chr4.3:89193384-89193403 | None:intergenic | 50.0% | |
| TGCCGCTGAATCTGTCTTGA+GGG | + | chr4.3:89193259-89193278 | MS.gene34317:CDS | 50.0% | |
| TTCCTGTTGATCCCCTTGAG+TGG | + | chr4.3:89193180-89193199 | MS.gene34317:CDS | 50.0% | |
| !! | GCTCTTGGAAGACTGGCTTT+GGG | + | chr4.3:89193637-89193656 | MS.gene34317:CDS | 50.0% |
| !! | TGCTCTTGGAAGACTGGCTT+TGG | + | chr4.3:89193636-89193655 | MS.gene34317:CDS | 50.0% |
| GTCTTGAGGGTTGCTGCTAC+TGG | + | chr4.3:89193272-89193291 | MS.gene34317:CDS | 55.0% | |
| TGCCACTCAAGGGGATCAAC+AGG | - | chr4.3:89193185-89193204 | None:intergenic | 55.0% | |
| ! | GTGCCGCTGAATCTGTCTTG+AGG | + | chr4.3:89193258-89193277 | MS.gene34317:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 89193180 | 89193679 | 89193180 | ID=MS.gene34317 |
| chr4.3 | mRNA | 89193180 | 89193679 | 89193180 | ID=MS.gene34317.t1;Parent=MS.gene34317 |
| chr4.3 | exon | 89193180 | 89193319 | 89193180 | ID=MS.gene34317.t1.exon1;Parent=MS.gene34317.t1 |
| chr4.3 | CDS | 89193180 | 89193319 | 89193180 | ID=cds.MS.gene34317.t1;Parent=MS.gene34317.t1 |
| chr4.3 | exon | 89193610 | 89193679 | 89193610 | ID=MS.gene34317.t1.exon2;Parent=MS.gene34317.t1 |
| chr4.3 | CDS | 89193610 | 89193679 | 89193610 | ID=cds.MS.gene34317.t1;Parent=MS.gene34317.t1 |
| Gene Sequence |
| Protein sequence |
