AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35501


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35501.t1 MTR_2g021690 95.912 318 13 0 1 318 1 318 0.0 622
MS.gene35501.t1 MTR_1g112160 54.508 244 104 6 63 301 8 249 1.08e-79 243
MS.gene35501.t1 MTR_1g112150 44.257 296 153 3 1 292 1 288 6.83e-78 240
MS.gene35501.t1 MTR_4g068340 35.036 274 156 6 1 262 1 264 2.98e-43 150
MS.gene35501.t1 MTR_8g071150 30.682 264 160 8 1 251 1 254 2.47e-33 124
MS.gene35501.t1 MTR_5g027180 25.641 273 191 5 1 268 1 266 1.12e-28 112
MS.gene35501.t1 MTR_8g071140 30.451 266 156 9 14 269 111 357 7.60e-26 105
MS.gene35501.t1 MTR_7g034135 25.641 195 134 3 129 312 106 300 1.64e-14 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35501.t1 AT1G55230 59.596 297 116 3 1 295 1 295 6.76e-124 357
MS.gene35501.t1 AT1G55240 57.372 312 128 4 1 309 1 310 1.03e-118 344
MS.gene35501.t1 AT5G19870 57.401 277 116 2 1 276 1 276 1.78e-105 309
MS.gene35501.t1 AT1G49470 32.095 296 180 7 1 284 1 287 1.10e-37 136
MS.gene35501.t1 AT1G32127 30.638 235 147 6 1 227 1 227 2.38e-30 116
MS.gene35501.t1 AT1G32127 30.638 235 147 6 1 227 1 227 2.38e-30 116
MS.gene35501.t1 AT3G01360 23.684 190 129 5 120 294 123 311 1.21e-11 65.1
MS.gene35501.t1 AT3G01360 23.684 190 129 5 120 294 123 311 1.21e-11 65.1

Find 64 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCTTTCAACTACCCTTT+TGG 0.147614 2.4:-65658331 MS.gene35501:CDS
AGGCTTGTGGCTTATGGTTA+TGG 0.190736 2.4:-65658247 MS.gene35501:CDS
CTTTCTTAATACTAGGATTA+TGG 0.209091 2.4:-65658776 MS.gene35501:CDS
CTTATTTGCACCATAATATT+TGG 0.212658 2.4:+65658038 None:intergenic
GTTGGTGAAATGTAGGAATT+TGG 0.216265 2.4:+65658717 None:intergenic
CCCAACATGAATAAAGCTTT+TGG 0.255141 2.4:+65657915 None:intergenic
CAAATATTATGGTGCAAATA+AGG 0.263519 2.4:-65658037 MS.gene35501:CDS
GGCTTGTGGCTTATGGTTAT+GGG 0.311665 2.4:-65658246 MS.gene35501:CDS
AGAAGATGGTATTGTCCTTC+TGG 0.334093 2.4:+65658375 None:intergenic
TGGAAGCTAGGAATTGTGTT+AGG 0.349655 2.4:+65658454 None:intergenic
TCGAAGGGTTGGTGTCGGTC+GGG 0.358375 2.4:+65658603 None:intergenic
TCATATATGGGCACTCTTGT+AGG 0.360041 2.4:-65658819 None:intergenic
CACCCTTTGGCAATTAAACT+TGG 0.365074 2.4:+65658207 None:intergenic
AAGGTTCGGTATTTCTCGTT+GGG 0.387451 2.4:-65658018 MS.gene35501:CDS
AGAATTCATAAAACACCCTT+TGG 0.389849 2.4:+65658194 None:intergenic
TGGTTATGGGGTACATGCTT+TGG 0.404676 2.4:-65658233 MS.gene35501:CDS
TACTACCTTTCCATGTCAAC+AGG 0.407875 2.4:+65657877 None:intergenic
CTGTTGAGGCTAACATGATT+AGG 0.407916 2.4:+65658655 None:intergenic
CCCAAAAGCTTTATTCATGT+TGG 0.411233 2.4:-65657916 MS.gene35501:CDS
ATCGAAGGGTTGGTGTCGGT+CGG 0.417234 2.4:+65658602 None:intergenic
CTCAACAGCTTCAATTTCAA+TGG 0.417853 2.4:-65658640 MS.gene35501:CDS
CCAAAAGCTTTATTCATGTT+GGG 0.426324 2.4:-65657915 MS.gene35501:CDS
GTATTTCTCGTTGGGGATTG+AGG 0.432650 2.4:-65658010 MS.gene35501:CDS
TGATGATGTGGAGTCTCAAA+AGG 0.433563 2.4:-65657956 MS.gene35501:CDS
CGAAGGGTTGGTGTCGGTCG+GGG 0.438020 2.4:+65658604 None:intergenic
TCTCAAAAGGGAAGCTTAGT+TGG 0.445309 2.4:-65657943 MS.gene35501:CDS
CGAACACGCTTTGATCTCCA+TGG 0.460449 2.4:-65658541 MS.gene35501:CDS
CTTGTAGGACATGTAGCTCC+TGG 0.486072 2.4:-65658804 MS.gene35501:CDS
GATTGTTCCGTCTGTATCGA+AGG 0.490701 2.4:+65658587 None:intergenic
TTTGTCAAGGATTATGGAGG+AGG 0.503244 2.4:+65658500 None:intergenic
CAAGAACTTGCAAGAGAAGA+TGG 0.509784 2.4:+65658361 None:intergenic
TAAGGTTCGGTATTTCTCGT+TGG 0.511002 2.4:-65658019 MS.gene35501:CDS
TTTGCATGGGAATTCCCAAA+AGG 0.511782 2.4:+65658316 None:intergenic
GGAGTTTGATGTGGTTGAAA+AGG 0.511822 2.4:+65658751 None:intergenic
TCAAAGCGTGTTCGAAGTTG+TGG 0.514973 2.4:+65658550 None:intergenic
TTCCGTCTGTATCGAAGGGT+TGG 0.515452 2.4:+65658592 None:intergenic
GATTATGGAGGAGGCGGCGT+AGG 0.527863 2.4:+65658509 None:intergenic
ATTATGGTGCAAATAAGGTT+CGG 0.528988 2.4:-65658032 MS.gene35501:CDS
CATTGAAATTGAAGCTGTTG+AGG 0.531529 2.4:+65658641 None:intergenic
GGAAGCTAGGAATTGTGTTA+GGG 0.534932 2.4:+65658455 None:intergenic
GTCAAGGATTATGGAGGAGG+CGG 0.536473 2.4:+65658503 None:intergenic
CACCAAGTTTAATTGCCAAA+GGG 0.536544 2.4:-65658209 MS.gene35501:CDS
TGTGATCAGCTGAATGAAGA+TGG 0.541577 2.4:+65658403 None:intergenic
TCTTCATTCAGCTGATCACA+TGG 0.543097 2.4:-65658400 MS.gene35501:CDS
ACATGATTAGGTAAAGTTCA+AGG 0.554269 2.4:+65658667 None:intergenic
TTTCACCTGTTGACATGGAA+AGG 0.555677 2.4:-65657882 MS.gene35501:CDS
TTGCATGGGAATTCCCAAAA+GGG 0.566598 2.4:+65658317 None:intergenic
ACACCAAGTTTAATTGCCAA+AGG 0.571150 2.4:-65658210 MS.gene35501:CDS
GCTTGTGGCTTATGGTTATG+GGG 0.571244 2.4:-65658245 MS.gene35501:CDS
AGGTTCGGTATTTCTCGTTG+GGG 0.581131 2.4:-65658017 MS.gene35501:CDS
ATTGTTCCGTCTGTATCGAA+GGG 0.588747 2.4:+65658588 None:intergenic
CTGTATCGAAGGGTTGGTGT+CGG 0.591425 2.4:+65658598 None:intergenic
GAACCATGTTGGTGAAATGT+AGG 0.593760 2.4:+65658710 None:intergenic
ATTCCTACATTTCACCAACA+TGG 0.602217 2.4:-65658713 MS.gene35501:CDS
GCTGATCACATGGGACCAGA+AGG 0.605989 2.4:-65658390 MS.gene35501:CDS
GATGATGTGGAGTCTCAAAA+GGG 0.613869 2.4:-65657955 MS.gene35501:CDS
CACCAACCCTTCGATACAGA+CGG 0.637019 2.4:-65658594 MS.gene35501:CDS
TTTGATGTTGAGAACCATGT+TGG 0.643972 2.4:+65658699 None:intergenic
AAGTTGTGGAGATGATACGA+GGG 0.657098 2.4:+65658564 None:intergenic
AGTATTAAGAAAGCAAAACC+AGG 0.657206 2.4:+65658786 None:intergenic
GAAGTTGTGGAGATGATACG+AGG 0.663722 2.4:+65658563 None:intergenic
CTTCATTCAGCTGATCACAT+GGG 0.667757 2.4:-65658399 MS.gene35501:CDS
GGCGTAGGTGAAGAAAGCCA+TGG 0.698512 2.4:+65658524 None:intergenic
AGTTGTGGAGATGATACGAG+GGG 0.713913 2.4:+65658565 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTAAGTATATTTTTTCA+AGG - chr2.4:65658425-65658444 MS.gene35501:CDS 15.0%
!!! AGGAATTTGGATTTTTAATA+TGG + chr2.4:65657965-65657984 None:intergenic 20.0%
!!! TAATATTTTGTTTAGTTGCT+TGG - chr2.4:65658586-65658605 MS.gene35501:CDS 20.0%
! ATACAAAGAAACACCAAAAA+CGG + chr2.4:65658624-65658643 None:intergenic 25.0%
! CAAATATTATGGTGCAAATA+AGG - chr2.4:65658655-65658674 MS.gene35501:CDS 25.0%
! CTTATTTGCACCATAATATT+TGG + chr2.4:65658657-65658676 None:intergenic 25.0%
! CTTTCTTAATACTAGGATTA+TGG - chr2.4:65657916-65657935 MS.gene35501:CDS 25.0%
!! ATTTCAATGGAACTTTTCAT+CGG - chr2.4:65658065-65658084 MS.gene35501:CDS 25.0%
!! TGAAAAATTAAGTGATGATG+TGG - chr2.4:65658724-65658743 MS.gene35501:CDS 25.0%
!! TTTTAATATGGAGTTTGATG+TGG + chr2.4:65657953-65657972 None:intergenic 25.0%
!!! TGTTTTATGAATTCTGAAGA+AGG - chr2.4:65658506-65658525 MS.gene35501:CDS 25.0%
!!! TTGGTTTTGACCAAATATTA+TGG - chr2.4:65658644-65658663 MS.gene35501:CDS 25.0%
!!! TTTTGGTGTTTCTTTGTATT+TGG - chr2.4:65658625-65658644 MS.gene35501:CDS 25.0%
AAGCTGTTGAAAAAAAGCTA+TGG + chr2.4:65658261-65658280 None:intergenic 30.0%
ACATGATTAGGTAAAGTTCA+AGG + chr2.4:65658028-65658047 None:intergenic 30.0%
AGAATTCATAAAACACCCTT+TGG + chr2.4:65658501-65658520 None:intergenic 30.0%
AGTATTAAGAAAGCAAAACC+AGG + chr2.4:65657909-65657928 None:intergenic 30.0%
ATTATGGTGCAAATAAGGTT+CGG - chr2.4:65658660-65658679 MS.gene35501:CDS 30.0%
! CCAACATGAATAAAGCTTTT+GGG + chr2.4:65658779-65658798 None:intergenic 30.0%
! GGTTTTGCTTTCTTAATACT+AGG - chr2.4:65657909-65657928 MS.gene35501:CDS 30.0%
! TTCGATTTTGTCAAGGATTA+TGG + chr2.4:65658201-65658220 None:intergenic 30.0%
!! CCAAAAGCTTTATTCATGTT+GGG - chr2.4:65658777-65658796 MS.gene35501:CDS 30.0%
!!! GTATATTTTTTCAAGGCTTG+TGG - chr2.4:65658432-65658451 MS.gene35501:CDS 30.0%
ACACCAAGTTTAATTGCCAA+AGG - chr2.4:65658482-65658501 MS.gene35501:CDS 35.0%
ATTCCTACATTTCACCAACA+TGG - chr2.4:65657979-65657998 MS.gene35501:CDS 35.0%
CACCAAGTTTAATTGCCAAA+GGG - chr2.4:65658483-65658502 MS.gene35501:CDS 35.0%
CATTGAAATTGAAGCTGTTG+AGG + chr2.4:65658054-65658073 None:intergenic 35.0%
CTCAACAGCTTCAATTTCAA+TGG - chr2.4:65658052-65658071 MS.gene35501:CDS 35.0%
GAAAAAAAGCTATGGAAGCT+AGG + chr2.4:65658253-65658272 None:intergenic 35.0%
TTTGATGTTGAGAACCATGT+TGG + chr2.4:65657996-65658015 None:intergenic 35.0%
! CCCAACATGAATAAAGCTTT+TGG + chr2.4:65658780-65658799 None:intergenic 35.0%
! GATTTTGTCAAGGATTATGG+AGG + chr2.4:65658198-65658217 None:intergenic 35.0%
! GTTGGTGAAATGTAGGAATT+TGG + chr2.4:65657978-65657997 None:intergenic 35.0%
! TCTCTTTCAACTACCCTTTT+GGG - chr2.4:65658362-65658381 MS.gene35501:CDS 35.0%
!! CCCAAAAGCTTTATTCATGT+TGG - chr2.4:65658776-65658795 MS.gene35501:CDS 35.0%
!! CTTGGTTTTCGATTTTGTCA+AGG + chr2.4:65658208-65658227 None:intergenic 35.0%
!!! AACCAAGAAGCTTTTTTGCA+TGG + chr2.4:65658393-65658412 None:intergenic 35.0%
!!! ACCAAGAAGCTTTTTTGCAT+GGG + chr2.4:65658392-65658411 None:intergenic 35.0%
!!! GTTATTGTTGTTGCCGTTTT+TGG - chr2.4:65658608-65658627 MS.gene35501:CDS 35.0%
AAGTTGTGGAGATGATACGA+GGG + chr2.4:65658131-65658150 None:intergenic 40.0%
AGAAGATGGTATTGTCCTTC+TGG + chr2.4:65658320-65658339 None:intergenic 40.0%
ATTGTTCCGTCTGTATCGAA+GGG + chr2.4:65658107-65658126 None:intergenic 40.0%
CAAGAACTTGCAAGAGAAGA+TGG + chr2.4:65658334-65658353 None:intergenic 40.0%
CACCCTTTGGCAATTAAACT+TGG + chr2.4:65658488-65658507 None:intergenic 40.0%
CTGTTGAGGCTAACATGATT+AGG + chr2.4:65658040-65658059 None:intergenic 40.0%
CTTCATTCAGCTGATCACAT+GGG - chr2.4:65658293-65658312 MS.gene35501:CDS 40.0%
GATGATGTGGAGTCTCAAAA+GGG - chr2.4:65658737-65658756 MS.gene35501:CDS 40.0%
GGAAGCTAGGAATTGTGTTA+GGG + chr2.4:65658240-65658259 None:intergenic 40.0%
GGAGTTTGATGTGGTTGAAA+AGG + chr2.4:65657944-65657963 None:intergenic 40.0%
TCCCATGCAAAAAAGCTTCT+TGG - chr2.4:65658388-65658407 MS.gene35501:CDS 40.0%
TCTCAAAAGGGAAGCTTAGT+TGG - chr2.4:65658749-65658768 MS.gene35501:CDS 40.0%
TCTTCATTCAGCTGATCACA+TGG - chr2.4:65658292-65658311 MS.gene35501:CDS 40.0%
TGGAAGCTAGGAATTGTGTT+AGG + chr2.4:65658241-65658260 None:intergenic 40.0%
TGTGATCAGCTGAATGAAGA+TGG + chr2.4:65658292-65658311 None:intergenic 40.0%
TTGCATGGGAATTCCCAAAA+GGG + chr2.4:65658378-65658397 None:intergenic 40.0%
TTTCACCTGTTGACATGGAA+AGG - chr2.4:65658810-65658829 MS.gene35501:CDS 40.0%
TTTGCATGGGAATTCCCAAA+AGG + chr2.4:65658379-65658398 None:intergenic 40.0%
TTTGTCAAGGATTATGGAGG+AGG + chr2.4:65658195-65658214 None:intergenic 40.0%
! AACGTTTTCACCTGTTGACA+TGG - chr2.4:65658805-65658824 MS.gene35501:CDS 40.0%
! AAGGTTCGGTATTTCTCGTT+GGG - chr2.4:65658674-65658693 MS.gene35501:CDS 40.0%
! CTCTCTTTCAACTACCCTTT+TGG - chr2.4:65658361-65658380 MS.gene35501:CDS 40.0%
! GAACCATGTTGGTGAAATGT+AGG + chr2.4:65657985-65658004 None:intergenic 40.0%
! TAAGGTTCGGTATTTCTCGT+TGG - chr2.4:65658673-65658692 MS.gene35501:CDS 40.0%
! TGATGATGTGGAGTCTCAAA+AGG - chr2.4:65658736-65658755 MS.gene35501:CDS 40.0%
!! TTTTCAAGGCTTGTGGCTTA+TGG - chr2.4:65658439-65658458 MS.gene35501:CDS 40.0%
AGGCTTGTGGCTTATGGTTA+TGG - chr2.4:65658445-65658464 MS.gene35501:CDS 45.0%
AGTTGTGGAGATGATACGAG+GGG + chr2.4:65658130-65658149 None:intergenic 45.0%
GAAGTTGTGGAGATGATACG+AGG + chr2.4:65658132-65658151 None:intergenic 45.0%
GATTGTTCCGTCTGTATCGA+AGG + chr2.4:65658108-65658127 None:intergenic 45.0%
GCTTGTGGCTTATGGTTATG+GGG - chr2.4:65658447-65658466 MS.gene35501:CDS 45.0%
GGCTTGTGGCTTATGGTTAT+GGG - chr2.4:65658446-65658465 MS.gene35501:CDS 45.0%
GTATTTCTCGTTGGGGATTG+AGG - chr2.4:65658682-65658701 MS.gene35501:CDS 45.0%
TCAAAGCGTGTTCGAAGTTG+TGG + chr2.4:65658145-65658164 None:intergenic 45.0%
TGGTTATGGGGTACATGCTT+TGG - chr2.4:65658459-65658478 MS.gene35501:CDS 45.0%
! AGGTTCGGTATTTCTCGTTG+GGG - chr2.4:65658675-65658694 MS.gene35501:CDS 45.0%
! GTGTTAGGGCAAGTTTTGCT+TGG + chr2.4:65658226-65658245 None:intergenic 45.0%
CACCAACCCTTCGATACAGA+CGG - chr2.4:65658098-65658117 MS.gene35501:CDS 50.0%
CGAACACGCTTTGATCTCCA+TGG - chr2.4:65658151-65658170 MS.gene35501:CDS 50.0%
CTTGTAGGACATGTAGCTCC+TGG - chr2.4:65657888-65657907 MS.gene35501:CDS 50.0%
GTCAAGGATTATGGAGGAGG+CGG + chr2.4:65658192-65658211 None:intergenic 50.0%
TTCCGTCTGTATCGAAGGGT+TGG + chr2.4:65658103-65658122 None:intergenic 50.0%
!! CTGTATCGAAGGGTTGGTGT+CGG + chr2.4:65658097-65658116 None:intergenic 50.0%
GCTGATCACATGGGACCAGA+AGG - chr2.4:65658302-65658321 MS.gene35501:CDS 55.0%
GGCGTAGGTGAAGAAAGCCA+TGG + chr2.4:65658171-65658190 None:intergenic 55.0%
!! ATCGAAGGGTTGGTGTCGGT+CGG + chr2.4:65658093-65658112 None:intergenic 55.0%
GATTATGGAGGAGGCGGCGT+AGG + chr2.4:65658186-65658205 None:intergenic 60.0%
!! TCGAAGGGTTGGTGTCGGTC+GGG + chr2.4:65658092-65658111 None:intergenic 60.0%
!! CGAAGGGTTGGTGTCGGTCG+GGG + chr2.4:65658091-65658110 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 65657879 65658835 65657879 ID=MS.gene35501
chr2.4 mRNA 65657879 65658835 65657879 ID=MS.gene35501.t1;Parent=MS.gene35501
chr2.4 exon 65657879 65658835 65657879 ID=MS.gene35501.t1.exon1;Parent=MS.gene35501.t1
chr2.4 CDS 65657879 65658835 65657879 ID=cds.MS.gene35501.t1;Parent=MS.gene35501.t1
Gene Sequence

>MS.gene35501

ATGGGCACTCTTGTAGGACATGTAGCTCCTGGTTTTGCTTTCTTAATACTAGGATTATGGCACCTTTTCAACCACATCAAACTCCATATTAAAAATCCAAATTCCTACATTTCACCAACATGGTTCTCAACATCAAAATTCAAATACCTTGAACTTTACCTAATCATGTTAGCCTCAACAGCTTCAATTTCAATGGAACTTTTCATCGGCCCCGACCGACACCAACCCTTCGATACAGACGGAACAATCCCCTCGTATCATCTCCACAACTTCGAACACGCTTTGATCTCCATGGCTTTCTTCACCTACGCCGCCTCCTCCATAATCCTTGACAAAATCGAAAACCAAGCAAAACTTGCCCTAACACAATTCCTAGCTTCCATAGCTTTTTTTCAACAGCTTCTTCTCTTCCATCTTCATTCAGCTGATCACATGGGACCAGAAGGACAATACCATCTTCTCTTGCAAGTTCTTGTCGTTGTCTCTCTTTCAACTACCCTTTTGGGAATTCCCATGCAAAAAAGCTTCTTGGTTAGTTTTGTGAGATCATTAAGTATATTTTTTCAAGGCTTGTGGCTTATGGTTATGGGGTACATGCTTTGGACACCAAGTTTAATTGCCAAAGGGTGTTTTATGAATTCTGAAGAAGGTCATAAAGTTGTGAGATGTTCAGATCAAGAATCACTTCATCGTGCTGTTTCACTTGTTAATATTTTGTTTAGTTGCTTGGTTATTGTTGTTGCCGTTTTTGGTGTTTCTTTGTATTTGGTTTTGACCAAATATTATGGTGCAAATAAGGTTCGGTATTTCTCGTTGGGGATTGAGGATGAAGAGAGAGAAGATGTTGAAAAATTAAGTGATGATGTGGAGTCTCAAAAGGGAAGCTTAGTTGGCAAGCCCAAAAGCTTTATTCATGTTGGGCAGAAAACGTTTTCACCTGTTGACATGGAAAGGTAG

Protein sequence

>MS.gene35501.t1

MGTLVGHVAPGFAFLILGLWHLFNHIKLHIKNPNSYISPTWFSTSKFKYLELYLIMLASTASISMELFIGPDRHQPFDTDGTIPSYHLHNFEHALISMAFFTYAASSIILDKIENQAKLALTQFLASIAFFQQLLLFHLHSADHMGPEGQYHLLLQVLVVVSLSTTLLGIPMQKSFLVSFVRSLSIFFQGLWLMVMGYMLWTPSLIAKGCFMNSEEGHKVVRCSDQESLHRAVSLVNILFSCLVIVVAVFGVSLYLVLTKYYGANKVRYFSLGIEDEEREDVEKLSDDVESQKGSLVGKPKSFIHVGQKTFSPVDMER