Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37852.t1 | XP_003617057.2 | 96.5 | 254 | 9 | 0 | 1 | 254 | 1 | 254 | 8.70E-90 | 340.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37852.t1 | D9UC01 | 35.2 | 88 | 56 | 1 | 14 | 100 | 16 | 103 | 5.9e-10 | 66.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37852.t1 | G7KHG4 | 96.5 | 254 | 9 | 0 | 1 | 254 | 1 | 254 | 6.3e-90 | 340.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene37852 | 0.831944 | 1.25E-55 | -1.69E-46 |
| MS.gene049227 | MS.gene37852 | 0.802582 | 5.24E-49 | -1.69E-46 |
| MS.gene049698 | MS.gene37852 | 0.807595 | 4.68E-50 | -1.69E-46 |
| MS.gene049711 | MS.gene37852 | 0.809815 | 1.57E-50 | -1.69E-46 |
| MS.gene05150 | MS.gene37852 | 0.803282 | 3.76E-49 | -1.69E-46 |
| MS.gene05155 | MS.gene37852 | 0.807636 | 4.59E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene37852 | 0.838256 | 3.20E-57 | -1.69E-46 |
| MS.gene052516 | MS.gene37852 | 0.801776 | 7.67E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene37852 | 0.815168 | 1.06E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene37852 | 0.824836 | 6.50E-54 | -1.69E-46 |
| MS.gene053581 | MS.gene37852 | 0.835001 | 2.16E-56 | -1.69E-46 |
| MS.gene054584 | MS.gene37852 | 0.820766 | 5.77E-53 | -1.69E-46 |
| MS.gene05458 | MS.gene37852 | 0.807855 | 4.12E-50 | -1.69E-46 |
| MS.gene055144 | MS.gene37852 | 0.857082 | 2.08E-62 | -1.69E-46 |
| MS.gene055465 | MS.gene37852 | 0.806645 | 7.44E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene37852 | 0.80512 | 1.56E-49 | -1.69E-46 |
| MS.gene055468 | MS.gene37852 | 0.808883 | 2.49E-50 | -1.69E-46 |
| MS.gene055469 | MS.gene37852 | 0.801722 | 7.87E-49 | -1.69E-46 |
| MS.gene056308 | MS.gene37852 | 0.820102 | 8.19E-53 | -1.69E-46 |
| MS.gene056507 | MS.gene37852 | 0.809068 | 2.27E-50 | -1.69E-46 |
| MS.gene058339 | MS.gene37852 | 0.872061 | 4.25E-67 | -1.69E-46 |
| MS.gene058351 | MS.gene37852 | 0.810787 | 9.69E-51 | -1.69E-46 |
| MS.gene058360 | MS.gene37852 | 0.810964 | 8.87E-51 | -1.69E-46 |
| MS.gene058852 | MS.gene37852 | 0.822093 | 2.85E-53 | -1.69E-46 |
| MS.gene058909 | MS.gene37852 | 0.836016 | 1.20E-56 | -1.69E-46 |
| MS.gene058912 | MS.gene37852 | 0.8301 | 3.55E-55 | -1.69E-46 |
| MS.gene061006 | MS.gene37852 | 0.8102 | 1.30E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene37852 | 0.80138 | 9.25E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37852.t1 | MTR_5g087450 | 97.244 | 254 | 7 | 0 | 1 | 254 | 1 | 254 | 0.0 | 505 |
| MS.gene37852.t1 | MTR_5g086020 | 93.701 | 127 | 8 | 0 | 62 | 188 | 1 | 127 | 1.11e-71 | 216 |
| MS.gene37852.t1 | MTR_5g087470 | 44.186 | 172 | 83 | 5 | 3 | 169 | 1 | 164 | 1.37e-35 | 128 |
| MS.gene37852.t1 | MTR_5g087480 | 36.082 | 194 | 91 | 6 | 3 | 176 | 1 | 181 | 5.05e-27 | 106 |
| MS.gene37852.t1 | MTR_5g087480 | 42.857 | 126 | 64 | 3 | 3 | 127 | 1 | 119 | 3.20e-26 | 105 |
| MS.gene37852.t1 | MTR_7g086130 | 39.785 | 93 | 56 | 0 | 11 | 103 | 8 | 100 | 5.16e-19 | 82.8 |
| MS.gene37852.t1 | MTR_6g478190 | 41.758 | 91 | 53 | 0 | 10 | 100 | 10 | 100 | 1.59e-18 | 80.5 |
| MS.gene37852.t1 | MTR_7g086130 | 37.755 | 98 | 56 | 1 | 11 | 103 | 8 | 105 | 8.14e-17 | 77.0 |
| MS.gene37852.t1 | MTR_4g107860 | 26.582 | 237 | 155 | 5 | 13 | 237 | 21 | 250 | 1.17e-16 | 77.4 |
| MS.gene37852.t1 | MTR_8g046245 | 36.667 | 90 | 56 | 1 | 12 | 100 | 22 | 111 | 4.91e-15 | 74.3 |
| MS.gene37852.t1 | MTR_1g076910 | 33.613 | 119 | 64 | 4 | 20 | 125 | 77 | 193 | 2.02e-12 | 64.7 |
| MS.gene37852.t1 | MTR_3g007660 | 29.508 | 122 | 75 | 3 | 13 | 123 | 19 | 140 | 5.76e-12 | 65.5 |
| MS.gene37852.t1 | MTR_1g076880 | 31.304 | 115 | 66 | 3 | 22 | 125 | 78 | 190 | 2.65e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37852.t1 | AT2G27140 | 37.640 | 178 | 95 | 4 | 10 | 174 | 10 | 184 | 5.62e-31 | 114 |
| MS.gene37852.t1 | AT5G20970 | 34.538 | 249 | 133 | 6 | 11 | 243 | 7 | 241 | 3.98e-28 | 108 |
| MS.gene37852.t1 | AT5G04890 | 35.227 | 88 | 56 | 1 | 14 | 100 | 16 | 103 | 5.37e-13 | 68.2 |
| MS.gene37852.t1 | AT1G54400 | 37.363 | 91 | 56 | 1 | 13 | 102 | 12 | 102 | 8.44e-13 | 65.5 |
| MS.gene37852.t1 | AT4G10250 | 37.363 | 91 | 46 | 2 | 22 | 101 | 74 | 164 | 1.17e-11 | 62.4 |
Find 41 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAGATACCAGCTTCAAATT+TGG | 0.240286 | 5.3:-70270735 | None:intergenic |
| ACTTCTTCAATTTCTTTGTT+TGG | 0.273292 | 5.3:-70270926 | None:intergenic |
| TTCTTGTTGTGTTGTTGATT+TGG | 0.305462 | 5.3:-70270810 | None:intergenic |
| GGTTTCTGAGGAATTGTTGC+TGG | 0.343124 | 5.3:-70270851 | None:intergenic |
| AAATTGAAGAAGTGTCTAAA+AGG | 0.356274 | 5.3:+70270936 | MS.gene37852:CDS |
| GAAGAAGCTGGTTAATTTGT+TGG | 0.370154 | 5.3:+70271126 | MS.gene37852:CDS |
| ACACTTATTCTTATGCTTCC+AGG | 0.372973 | 5.3:+70270355 | MS.gene37852:CDS |
| AAAGTCATTGACTATGGAAA+TGG | 0.386319 | 5.3:+70271039 | MS.gene37852:CDS |
| AATTTGAACCACCCTCTGAT+TGG | 0.401334 | 5.3:+70270311 | MS.gene37852:CDS |
| AATTAATCAATTACAACTCT+TGG | 0.411666 | 5.3:-70271228 | None:intergenic |
| GAAACTTGAGGAGCAGTGTT+TGG | 0.417491 | 5.3:-70270872 | None:intergenic |
| GTTGTTGATTTGGTTGGTGT+TGG | 0.422621 | 5.3:-70270800 | None:intergenic |
| TGTTGTGTTGTTGATTTGGT+TGG | 0.444857 | 5.3:-70270806 | None:intergenic |
| ATTTGAACCACCCTCTGATT+GGG | 0.445092 | 5.3:+70270312 | MS.gene37852:CDS |
| ACCCGTGTGCTAAGGGTTAG+TGG | 0.463763 | 5.3:+70270626 | MS.gene37852:CDS |
| AACCCTTAGCACACGGGTTG+AGG | 0.474881 | 5.3:-70270621 | None:intergenic |
| GTGTCTAAAAGGAAGGGAAA+AGG | 0.475085 | 5.3:+70270947 | MS.gene37852:CDS |
| TGAAGAAGTGTCTAAAAGGA+AGG | 0.482631 | 5.3:+70270940 | MS.gene37852:CDS |
| TGTGAATGCATTATGTGGTT+TGG | 0.495211 | 5.3:+70271084 | MS.gene37852:CDS |
| TAACCTCAACCCGTGTGCTA+AGG | 0.497781 | 5.3:+70270618 | MS.gene37852:CDS |
| CACCACTAACCCTTAGCACA+CGG | 0.507093 | 5.3:-70270628 | None:intergenic |
| ATTTAAGAAGGATCAATTGA+GGG | 0.509297 | 5.3:+70270589 | MS.gene37852:CDS |
| GGAATTGTTGCTGGTTGTTG+AGG | 0.516048 | 5.3:-70270842 | None:intergenic |
| ATTGGTGCCAAATTTGAAGC+TGG | 0.520171 | 5.3:+70270728 | MS.gene37852:CDS |
| GAATGCATTATGTGGTTTGG+TGG | 0.533238 | 5.3:+70271087 | MS.gene37852:CDS |
| GATTTAAGAAGGATCAATTG+AGG | 0.534085 | 5.3:+70270588 | MS.gene37852:CDS |
| AATACATGCAGGATTTAAGA+AGG | 0.538421 | 5.3:+70270577 | MS.gene37852:intron |
| AACCTCAACCCGTGTGCTAA+GGG | 0.546287 | 5.3:+70270619 | MS.gene37852:CDS |
| AGTTAAGAAGCAGAAGAAGC+TGG | 0.549223 | 5.3:+70271114 | MS.gene37852:CDS |
| GGAGCAGTGTTTGGTTTCTG+AGG | 0.565721 | 5.3:-70270863 | None:intergenic |
| CATTCTGACACTAGTAACAT+TGG | 0.571128 | 5.3:+70270710 | MS.gene37852:CDS |
| ACCACTAACCCTTAGCACAC+GGG | 0.576619 | 5.3:-70270627 | None:intergenic |
| GAATATGTGAATGCATTATG+TGG | 0.588342 | 5.3:+70271079 | MS.gene37852:CDS |
| GAAGAAGTGTCTAAAAGGAA+GGG | 0.593904 | 5.3:+70270941 | MS.gene37852:CDS |
| ACCCTCTGATTGGGATCATG+AGG | 0.602046 | 5.3:+70270321 | MS.gene37852:CDS |
| TCCTCATGATCCCAATCAGA+GGG | 0.610175 | 5.3:-70270322 | None:intergenic |
| ATGTTACTAGTGTCAGAATG+AGG | 0.615006 | 5.3:-70270707 | None:intergenic |
| GAAGGGAAAAGGTAAAGCTG+AGG | 0.645097 | 5.3:+70270958 | MS.gene37852:CDS |
| ATCCTCATGATCCCAATCAG+AGG | 0.659662 | 5.3:-70270323 | None:intergenic |
| TTACTAGTGTCAGAATGAGG+TGG | 0.686289 | 5.3:-70270704 | None:intergenic |
| TCATGATCCCAATCAGAGGG+TGG | 0.690466 | 5.3:-70270319 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTAATTAATTCATTTTTTTC+AGG | + | chr5.3:70271193-70271212 | MS.gene37852:CDS | 15.0% |
| !!! | ATTAATTCATTTTTTTCAGG+AGG | + | chr5.3:70271196-70271215 | MS.gene37852:CDS | 20.0% |
| ! | ACTTCTTCAATTTCTTTGTT+TGG | - | chr5.3:70270929-70270948 | None:intergenic | 25.0% |
| ! | ATTTAAGAAGGATCAATTGA+GGG | + | chr5.3:70270589-70270608 | MS.gene37852:CDS | 25.0% |
| ! | GACAAATATATAAGTATACG+AGG | + | chr5.3:70270503-70270522 | MS.gene37852:intron | 25.0% |
| ! | GACAAATGAATGAGAAAAAA+TGG | + | chr5.3:70270654-70270673 | MS.gene37852:CDS | 25.0% |
| ! | TATTATATGAAAAAGACACG+AGG | - | chr5.3:70270399-70270418 | None:intergenic | 25.0% |
| !! | TGAAAAAAAGTCATTGACTA+TGG | + | chr5.3:70271033-70271052 | MS.gene37852:CDS | 25.0% |
| !!! | AAATTGAAGAAGTGTCTAAA+AGG | + | chr5.3:70270936-70270955 | MS.gene37852:CDS | 25.0% |
| AAGAAAGAAATGAACTAACC+TGG | - | chr5.3:70270376-70270395 | None:intergenic | 30.0% | |
| AATACATGCAGGATTTAAGA+AGG | + | chr5.3:70270577-70270596 | MS.gene37852:intron | 30.0% | |
| GAATATGTGAATGCATTATG+TGG | + | chr5.3:70271079-70271098 | MS.gene37852:CDS | 30.0% | |
| GATTTAAGAAGGATCAATTG+AGG | + | chr5.3:70270588-70270607 | MS.gene37852:CDS | 30.0% | |
| TAAGATACCAGCTTCAAATT+TGG | - | chr5.3:70270738-70270757 | None:intergenic | 30.0% | |
| ! | AAAGTCATTGACTATGGAAA+TGG | + | chr5.3:70271039-70271058 | MS.gene37852:CDS | 30.0% |
| ! | GTGTTGGAACAATATTTTGT+TGG | - | chr5.3:70270787-70270806 | None:intergenic | 30.0% |
| ! | TTCTTGTTGTGTTGTTGATT+TGG | - | chr5.3:70270813-70270832 | None:intergenic | 30.0% |
| !!! | ATTTTGTTGGTTGATGAGTT+TGG | - | chr5.3:70270774-70270793 | None:intergenic | 30.0% |
| !!! | GATGTACTACTTGTTTTTTC+TGG | - | chr5.3:70270901-70270920 | None:intergenic | 30.0% |
| !!! | TTTTGTTGGTTGATGAGTTT+GGG | - | chr5.3:70270773-70270792 | None:intergenic | 30.0% |
| ACACTTATTCTTATGCTTCC+AGG | + | chr5.3:70270355-70270374 | MS.gene37852:CDS | 35.0% | |
| ATGTTACTAGTGTCAGAATG+AGG | - | chr5.3:70270710-70270729 | None:intergenic | 35.0% | |
| CATTCTGACACTAGTAACAT+TGG | + | chr5.3:70270710-70270729 | MS.gene37852:CDS | 35.0% | |
| CTATGATCGATAATACATGC+AGG | + | chr5.3:70270566-70270585 | MS.gene37852:intron | 35.0% | |
| TGTGAATGCATTATGTGGTT+TGG | + | chr5.3:70271084-70271103 | MS.gene37852:CDS | 35.0% | |
| !! | GAAGAAGCTGGTTAATTTGT+TGG | + | chr5.3:70271126-70271145 | MS.gene37852:CDS | 35.0% |
| !! | GAAGAAGTGTCTAAAAGGAA+GGG | + | chr5.3:70270941-70270960 | MS.gene37852:CDS | 35.0% |
| !! | TGAAGAAGTGTCTAAAAGGA+AGG | + | chr5.3:70270940-70270959 | MS.gene37852:CDS | 35.0% |
| !! | TGTTGTGTTGTTGATTTGGT+TGG | - | chr5.3:70270809-70270828 | None:intergenic | 35.0% |
| !! | TTTGTTGGTGCTTGTGTTTT+TGG | + | chr5.3:70271141-70271160 | MS.gene37852:CDS | 35.0% |
| !! | TTTTTCTGGTCAGAAACTTG+AGG | - | chr5.3:70270887-70270906 | None:intergenic | 35.0% |
| AATTTGAACCACCCTCTGAT+TGG | + | chr5.3:70270311-70270330 | MS.gene37852:CDS | 40.0% | |
| AGTTAAGAAGCAGAAGAAGC+TGG | + | chr5.3:70271114-70271133 | MS.gene37852:CDS | 40.0% | |
| ATTTGAACCACCCTCTGATT+GGG | + | chr5.3:70270312-70270331 | MS.gene37852:CDS | 40.0% | |
| GTGTCTAAAAGGAAGGGAAA+AGG | + | chr5.3:70270947-70270966 | MS.gene37852:CDS | 40.0% | |
| TTACTAGTGTCAGAATGAGG+TGG | - | chr5.3:70270707-70270726 | None:intergenic | 40.0% | |
| ! | GAATGCATTATGTGGTTTGG+TGG | + | chr5.3:70271087-70271106 | MS.gene37852:CDS | 40.0% |
| !! | ATTGGTGCCAAATTTGAAGC+TGG | + | chr5.3:70270728-70270747 | MS.gene37852:CDS | 40.0% |
| !! | GTTGTTGATTTGGTTGGTGT+TGG | - | chr5.3:70270803-70270822 | None:intergenic | 40.0% |
| !!! | GTGTTTTTGGTCTTGCTGTT+TGG | + | chr5.3:70271154-70271173 | MS.gene37852:CDS | 40.0% |
| ATCCTCATGATCCCAATCAG+AGG | - | chr5.3:70270326-70270345 | None:intergenic | 45.0% | |
| GAAACTTGAGGAGCAGTGTT+TGG | - | chr5.3:70270875-70270894 | None:intergenic | 45.0% | |
| GAAGGGAAAAGGTAAAGCTG+AGG | + | chr5.3:70270958-70270977 | MS.gene37852:CDS | 45.0% | |
| TCCTCATGATCCCAATCAGA+GGG | - | chr5.3:70270325-70270344 | None:intergenic | 45.0% | |
| ! | GGAATTGTTGCTGGTTGTTG+AGG | - | chr5.3:70270845-70270864 | None:intergenic | 45.0% |
| ! | GGTTTCTGAGGAATTGTTGC+TGG | - | chr5.3:70270854-70270873 | None:intergenic | 45.0% |
| AACCTCAACCCGTGTGCTAA+GGG | + | chr5.3:70270619-70270638 | MS.gene37852:CDS | 50.0% | |
| ACCACTAACCCTTAGCACAC+GGG | - | chr5.3:70270630-70270649 | None:intergenic | 50.0% | |
| ACCCTCTGATTGGGATCATG+AGG | + | chr5.3:70270321-70270340 | MS.gene37852:CDS | 50.0% | |
| CACCACTAACCCTTAGCACA+CGG | - | chr5.3:70270631-70270650 | None:intergenic | 50.0% | |
| TAACCTCAACCCGTGTGCTA+AGG | + | chr5.3:70270618-70270637 | MS.gene37852:CDS | 50.0% | |
| TCATGATCCCAATCAGAGGG+TGG | - | chr5.3:70270322-70270341 | None:intergenic | 50.0% | |
| ! | GCTGGTTGTTGAGGCTTTTG+AGG | - | chr5.3:70270836-70270855 | None:intergenic | 50.0% |
| ! | GGAGCAGTGTTTGGTTTCTG+AGG | - | chr5.3:70270866-70270885 | None:intergenic | 50.0% |
| ACCCGTGTGCTAAGGGTTAG+TGG | + | chr5.3:70270626-70270645 | MS.gene37852:CDS | 55.0% | |
| ! | AACCCTTAGCACACGGGTTG+AGG | - | chr5.3:70270624-70270643 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 70270265 | 70271240 | 70270265 | ID=MS.gene37852 |
| chr5.3 | mRNA | 70270265 | 70271240 | 70270265 | ID=MS.gene37852.t1;Parent=MS.gene37852 |
| chr5.3 | exon | 70270265 | 70270376 | 70270265 | ID=MS.gene37852.t1.exon1;Parent=MS.gene37852.t1 |
| chr5.3 | CDS | 70270265 | 70270376 | 70270265 | ID=cds.MS.gene37852.t1;Parent=MS.gene37852.t1 |
| chr5.3 | exon | 70270588 | 70271240 | 70270588 | ID=MS.gene37852.t1.exon2;Parent=MS.gene37852.t1 |
| chr5.3 | CDS | 70270588 | 70271240 | 70270588 | ID=cds.MS.gene37852.t1;Parent=MS.gene37852.t1 |
| Gene Sequence |
| Protein sequence |
