Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39378.t1 | XP_003626766.1 | 100 | 115 | 0 | 0 | 1 | 115 | 1 | 115 | 1.70E-56 | 228.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39378.t1 | Q8VZM9 | 48.2 | 110 | 48 | 2 | 15 | 115 | 50 | 159 | 1.1e-19 | 97.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39378.t1 | G7LCJ1 | 100.0 | 115 | 0 | 0 | 1 | 115 | 1 | 115 | 1.2e-56 | 228.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049190 | MS.gene39378 | 0.814607 | 1.41E-51 | -1.69E-46 |
| MS.gene049341 | MS.gene39378 | 0.836195 | 1.08E-56 | -1.69E-46 |
| MS.gene049404 | MS.gene39378 | 0.812183 | 4.82E-51 | -1.69E-46 |
| MS.gene049824 | MS.gene39378 | 0.805961 | 1.04E-49 | -1.69E-46 |
| MS.gene05016 | MS.gene39378 | 0.807317 | 5.36E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene39378 | 0.839723 | 1.34E-57 | -1.69E-46 |
| MS.gene050808 | MS.gene39378 | 0.806269 | 8.93E-50 | -1.69E-46 |
| MS.gene050959 | MS.gene39378 | 0.814267 | 1.68E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene39378 | 0.813657 | 2.29E-51 | -1.69E-46 |
| MS.gene051700 | MS.gene39378 | 0.805142 | 1.54E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene39378 | 0.832161 | 1.11E-55 | -1.69E-46 |
| MS.gene051720 | MS.gene39378 | 0.84462 | 6.75E-59 | -1.69E-46 |
| MS.gene051744 | MS.gene39378 | 0.807659 | 4.54E-50 | -1.69E-46 |
| MS.gene052133 | MS.gene39378 | 0.811998 | 5.29E-51 | -1.69E-46 |
| MS.gene052516 | MS.gene39378 | 0.832893 | 7.29E-56 | -1.69E-46 |
| MS.gene052665 | MS.gene39378 | 0.800334 | 1.51E-48 | -1.69E-46 |
| MS.gene052732 | MS.gene39378 | 0.810317 | 1.22E-50 | -1.69E-46 |
| MS.gene052879 | MS.gene39378 | 0.808151 | 3.57E-50 | -1.69E-46 |
| MS.gene053144 | MS.gene39378 | 0.859891 | 3.02E-63 | -1.69E-46 |
| MS.gene053174 | MS.gene39378 | 0.813989 | 1.94E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene39378 | 0.81046 | 1.14E-50 | -1.69E-46 |
| MS.gene053581 | MS.gene39378 | 0.808712 | 2.71E-50 | -1.69E-46 |
| MS.gene053599 | MS.gene39378 | 0.809106 | 2.23E-50 | -1.69E-46 |
| MS.gene054550 | MS.gene39378 | 0.824677 | 7.09E-54 | -1.69E-46 |
| MS.gene054584 | MS.gene39378 | 0.848247 | 6.92E-60 | -1.69E-46 |
| MS.gene055465 | MS.gene39378 | 0.826835 | 2.18E-54 | -1.69E-46 |
| MS.gene055466 | MS.gene39378 | 0.834219 | 3.40E-56 | -1.69E-46 |
| MS.gene055468 | MS.gene39378 | 0.814643 | 1.39E-51 | -1.69E-46 |
| MS.gene055512 | MS.gene39378 | 0.834251 | 3.34E-56 | -1.69E-46 |
| MS.gene055537 | MS.gene39378 | 0.837127 | 6.24E-57 | -1.69E-46 |
| MS.gene055580 | MS.gene39378 | -0.837557 | 4.85E-57 | -1.69E-46 |
| MS.gene055890 | MS.gene39378 | 0.84482 | 5.97E-59 | -1.69E-46 |
| MS.gene055891 | MS.gene39378 | 0.855767 | 5.06E-62 | -1.69E-46 |
| MS.gene056005 | MS.gene39378 | 0.815779 | 7.76E-52 | -1.69E-46 |
| MS.gene056043 | MS.gene39378 | -0.822642 | 2.12E-53 | -1.69E-46 |
| MS.gene056366 | MS.gene39378 | 0.801534 | 8.61E-49 | -1.69E-46 |
| MS.gene056553 | MS.gene39378 | 0.807304 | 5.40E-50 | -1.69E-46 |
| MS.gene058339 | MS.gene39378 | 0.805112 | 1.56E-49 | -1.69E-46 |
| MS.gene059015 | MS.gene39378 | 0.818982 | 1.47E-52 | -1.69E-46 |
| MS.gene059154 | MS.gene39378 | 0.803406 | 3.54E-49 | -1.69E-46 |
| MS.gene059937 | MS.gene39378 | 0.802272 | 6.07E-49 | -1.69E-46 |
| MS.gene060587 | MS.gene39378 | 0.84409 | 9.38E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39378.t1 | MTR_8g008840 | 100.000 | 115 | 0 | 0 | 1 | 115 | 1 | 115 | 3.65e-83 | 238 |
| MS.gene39378.t1 | MTR_4g099050 | 52.830 | 106 | 47 | 2 | 1 | 105 | 38 | 141 | 4.78e-32 | 110 |
| MS.gene39378.t1 | MTR_5g013770 | 56.311 | 103 | 37 | 4 | 18 | 115 | 66 | 165 | 6.80e-31 | 107 |
| MS.gene39378.t1 | MTR_3g087700 | 53.571 | 56 | 25 | 1 | 32 | 87 | 118 | 172 | 1.07e-15 | 69.7 |
| MS.gene39378.t1 | MTR_8g022950 | 57.143 | 56 | 22 | 1 | 38 | 93 | 39 | 92 | 2.77e-15 | 67.0 |
| MS.gene39378.t1 | MTR_3g073960 | 48.148 | 54 | 28 | 0 | 29 | 82 | 81 | 134 | 2.97e-14 | 65.1 |
| MS.gene39378.t1 | MTR_2g042970 | 57.407 | 54 | 22 | 1 | 29 | 82 | 177 | 229 | 1.48e-13 | 64.7 |
| MS.gene39378.t1 | MTR_2g436020 | 44.444 | 72 | 31 | 2 | 14 | 78 | 35 | 104 | 2.81e-12 | 59.7 |
| MS.gene39378.t1 | MTR_2g436250 | 44.444 | 72 | 31 | 2 | 14 | 78 | 35 | 104 | 2.81e-12 | 59.7 |
| MS.gene39378.t1 | MTR_7g095170 | 53.659 | 41 | 19 | 0 | 37 | 77 | 223 | 263 | 7.15e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene39378.t1 | AT2G44670 | 54.878 | 82 | 37 | 0 | 34 | 115 | 12 | 93 | 2.43e-29 | 102 |
| MS.gene39378.t1 | AT4G17670 | 47.368 | 114 | 41 | 4 | 16 | 115 | 51 | 159 | 4.78e-27 | 98.2 |
| MS.gene39378.t1 | AT5G47060 | 53.571 | 84 | 36 | 2 | 35 | 115 | 94 | 177 | 1.07e-25 | 95.1 |
| MS.gene39378.t1 | AT5G47060 | 53.571 | 84 | 36 | 2 | 35 | 115 | 94 | 177 | 1.07e-25 | 95.1 |
| MS.gene39378.t1 | AT1G78020 | 48.529 | 68 | 33 | 1 | 15 | 82 | 68 | 133 | 1.04e-17 | 74.3 |
| MS.gene39378.t1 | AT5G65040 | 47.541 | 61 | 30 | 1 | 33 | 93 | 54 | 112 | 1.97e-16 | 69.7 |
| MS.gene39378.t1 | AT1G22160 | 44.118 | 68 | 34 | 2 | 15 | 82 | 59 | 122 | 7.32e-15 | 66.6 |
| MS.gene39378.t1 | AT1G53885 | 55.319 | 47 | 21 | 0 | 35 | 81 | 39 | 85 | 3.55e-14 | 64.3 |
| MS.gene39378.t1 | AT1G53903 | 55.319 | 47 | 21 | 0 | 35 | 81 | 39 | 85 | 3.55e-14 | 64.3 |
| MS.gene39378.t1 | AT5G49120 | 50.000 | 46 | 23 | 0 | 33 | 78 | 63 | 108 | 3.40e-11 | 57.0 |
| MS.gene39378.t1 | AT5G20700 | 57.778 | 45 | 18 | 1 | 38 | 82 | 182 | 225 | 4.92e-11 | 58.2 |
Find 17 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATAAAACATGCTAGATTT+TGG | 0.214267 | 8.2:-81953481 | None:intergenic |
| AGAATTTCTAACACCTCTTT+TGG | 0.295222 | 8.2:-81954022 | None:intergenic |
| ACATAAAGATGTCTTTGTTT+TGG | 0.307802 | 8.2:-81953576 | None:intergenic |
| TCAGAATTCTTCAAACAATA+AGG | 0.335402 | 8.2:+81954053 | MS.gene39378:CDS |
| ATTGTTTGAAGAATTCTGAT+TGG | 0.437983 | 8.2:-81954049 | None:intergenic |
| ATGAATCCAAAGAGAAAAGT+TGG | 0.445108 | 8.2:+81953989 | MS.gene39378:CDS |
| TTCTACAAAGATAACAAGCT+TGG | 0.448464 | 8.2:-81953543 | None:intergenic |
| GGAGAAGGGATTGAAGAAGA+TGG | 0.454187 | 8.2:-81953460 | None:intergenic |
| TGGAAGATTTCAACCAAAAG+AGG | 0.483181 | 8.2:+81954009 | MS.gene39378:CDS |
| AAAGAATAGTTAGCAGCCAT+TGG | 0.486211 | 8.2:-81953424 | None:intergenic |
| ACAAAGACATCTTTATGTAC+AGG | 0.489194 | 8.2:+81953581 | MS.gene39378:CDS |
| TACAAAGATAACAAGCTTGG+AGG | 0.501824 | 8.2:-81953540 | None:intergenic |
| TATCTTTGTAGAAAACCTCT+TGG | 0.507181 | 8.2:+81953553 | MS.gene39378:CDS |
| CTGCACTCATTGCTACAGAA+TGG | 0.599412 | 8.2:-81953946 | None:intergenic |
| CATTCTGTAGCAATGAGTGC+AGG | 0.607408 | 8.2:+81953947 | MS.gene39378:CDS |
| AAGATCAGAATCAGTTGCAG+TGG | 0.640103 | 8.2:+81954080 | MS.gene39378:CDS |
| TAACAAGCTTGGAGGAAGTG+AGG | 0.645660 | 8.2:-81953532 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGATGTAAAATAATTATT+TGG | + | chr8.2:81953828-81953847 | MS.gene39378:intron | 10.0% |
| !!! | AGAGAACTTTTTTAATTATT+TGG | + | chr8.2:81953857-81953876 | MS.gene39378:intron | 15.0% |
| !! | AAAGTATAAATATTCATCTG+GGG | - | chr8.2:81953654-81953673 | None:intergenic | 20.0% |
| !! | ACAAAGTATAAATATTCATC+TGG | - | chr8.2:81953656-81953675 | None:intergenic | 20.0% |
| !! | CAAAGTATAAATATTCATCT+GGG | - | chr8.2:81953655-81953674 | None:intergenic | 20.0% |
| !!! | AAATCTAGCATGTTTTATTA+TGG | + | chr8.2:81953484-81953503 | MS.gene39378:CDS | 20.0% |
| !!! | TAATAAAACATGCTAGATTT+TGG | - | chr8.2:81953484-81953503 | None:intergenic | 20.0% |
| !!! | TATAATTGTGATTTTTCAGA+GGG | + | chr8.2:81953917-81953936 | MS.gene39378:intron | 20.0% |
| !!! | TTATAATTGTGATTTTTCAG+AGG | + | chr8.2:81953916-81953935 | MS.gene39378:intron | 20.0% |
| !!! | TTTACACTTAATTTTCATGT+AGG | + | chr8.2:81953676-81953695 | MS.gene39378:intron | 20.0% |
| ! | AAAAAGATGAAGGAAAATTG+AGG | - | chr8.2:81953625-81953644 | None:intergenic | 25.0% |
| ! | ATTGTTTGAAGAATTCTGAT+TGG | - | chr8.2:81954052-81954071 | None:intergenic | 25.0% |
| ! | TCAGAATTCTTCAAACAATA+AGG | + | chr8.2:81954053-81954072 | MS.gene39378:CDS | 25.0% |
| !!! | ACATAAAGATGTCTTTGTTT+TGG | - | chr8.2:81953579-81953598 | None:intergenic | 25.0% |
| ACAAAGACATCTTTATGTAC+AGG | + | chr8.2:81953581-81953600 | MS.gene39378:CDS | 30.0% | |
| ATGAATCCAAAGAGAAAAGT+TGG | + | chr8.2:81953989-81954008 | MS.gene39378:CDS | 30.0% | |
| GTAGGTCATAACTCATAATA+AGG | + | chr8.2:81953694-81953713 | MS.gene39378:intron | 30.0% | |
| TATCTTTGTAGAAAACCTCT+TGG | + | chr8.2:81953553-81953572 | MS.gene39378:CDS | 30.0% | |
| TTCTACAAAGATAACAAGCT+TGG | - | chr8.2:81953546-81953565 | None:intergenic | 30.0% | |
| ! | AATCTTCCAACTTTTCTCTT+TGG | - | chr8.2:81953998-81954017 | None:intergenic | 30.0% |
| ! | AGAATTTCTAACACCTCTTT+TGG | - | chr8.2:81954025-81954044 | None:intergenic | 30.0% |
| !! | TCTAGCATGTTTTATTATGG+TGG | + | chr8.2:81953487-81953506 | MS.gene39378:CDS | 30.0% |
| TACAAAGATAACAAGCTTGG+AGG | - | chr8.2:81953543-81953562 | None:intergenic | 35.0% | |
| TGGAAGATTTCAACCAAAAG+AGG | + | chr8.2:81954009-81954028 | MS.gene39378:CDS | 35.0% | |
| !! | AACATGCTAGATTTTGGAGA+AGG | - | chr8.2:81953478-81953497 | None:intergenic | 35.0% |
| !! | ACATGCTAGATTTTGGAGAA+GGG | - | chr8.2:81953477-81953496 | None:intergenic | 35.0% |
| AAGATCAGAATCAGTTGCAG+TGG | + | chr8.2:81954080-81954099 | MS.gene39378:CDS | 40.0% | |
| !!! | ATGTCTTTGTTTTGGCCAAG+AGG | - | chr8.2:81953571-81953590 | None:intergenic | 40.0% |
| CATTCTGTAGCAATGAGTGC+AGG | + | chr8.2:81953947-81953966 | MS.gene39378:CDS | 45.0% | |
| CTGCACTCATTGCTACAGAA+TGG | - | chr8.2:81953949-81953968 | None:intergenic | 45.0% | |
| GGAGAAGGGATTGAAGAAGA+TGG | - | chr8.2:81953463-81953482 | None:intergenic | 45.0% | |
| GGGGTGTGACAAAAAGATGA+AGG | - | chr8.2:81953635-81953654 | None:intergenic | 45.0% | |
| TAACAAGCTTGGAGGAAGTG+AGG | - | chr8.2:81953535-81953554 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 81953427 | 81954107 | 81953427 | ID=MS.gene39378 |
| chr8.2 | mRNA | 81953427 | 81954107 | 81953427 | ID=MS.gene39378.t1;Parent=MS.gene39378 |
| chr8.2 | exon | 81953427 | 81953602 | 81953427 | ID=MS.gene39378.t1.exon1;Parent=MS.gene39378.t1 |
| chr8.2 | CDS | 81953427 | 81953602 | 81953427 | ID=cds.MS.gene39378.t1;Parent=MS.gene39378.t1 |
| chr8.2 | exon | 81953936 | 81954107 | 81953936 | ID=MS.gene39378.t1.exon2;Parent=MS.gene39378.t1 |
| chr8.2 | CDS | 81953936 | 81954107 | 81953936 | ID=cds.MS.gene39378.t1;Parent=MS.gene39378.t1 |
| Gene Sequence |
| Protein sequence |
