AlfalfaGEDB | MS.gene39518

M. sativa cultivar XinJiangDaYe / MS.gene39518

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39518.t1	XP_013456959.1	90.3	359	34	1	1	359	1	358	2.50E-90	342.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39518.t1	Q01197	40.5	131	64	5	8	127	10	137	1.3e-07	58.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39518.t1	I3T3J6	90.3	359	34	1	1	359	1	358	1.8e-90	342.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049418	MS.gene39518	0.80554	1.27E-49	-1.69E-46
MS.gene049711	MS.gene39518	0.829814	4.17E-55	-1.69E-46
MS.gene050657	MS.gene39518	0.825143	5.51E-54	-1.69E-46
MS.gene051018	MS.gene39518	0.831488	1.62E-55	-1.69E-46
MS.gene051021	MS.gene39518	0.829364	5.37E-55	-1.69E-46
MS.gene051279	MS.gene39518	0.813648	2.30E-51	-1.69E-46
MS.gene051664	MS.gene39518	0.817129	3.87E-52	-1.69E-46
MS.gene051719	MS.gene39518	0.827423	1.58E-54	-1.69E-46
MS.gene051720	MS.gene39518	0.850681	1.45E-60	-1.69E-46
MS.gene052516	MS.gene39518	0.803415	3.52E-49	-1.69E-46
MS.gene052732	MS.gene39518	0.813999	1.93E-51	-1.69E-46
MS.gene053144	MS.gene39518	0.87492	4.63E-68	-1.69E-46
MS.gene053581	MS.gene39518	0.813353	2.67E-51	-1.69E-46
MS.gene054584	MS.gene39518	0.875399	3.18E-68	-1.69E-46
MS.gene055083	MS.gene39518	0.812479	4.15E-51	-1.69E-46
MS.gene055465	MS.gene39518	0.850449	1.69E-60	-1.69E-46
MS.gene055466	MS.gene39518	0.844898	5.68E-59	-1.69E-46
MS.gene055468	MS.gene39518	0.818816	1.61E-52	-1.69E-46
MS.gene055469	MS.gene39518	0.825253	5.18E-54	-1.69E-46
MS.gene055537	MS.gene39518	0.866242	3.29E-65	-1.69E-46
MS.gene055580	MS.gene39518	-0.836369	9.75E-57	-1.69E-46
MS.gene055890	MS.gene39518	0.81766	2.94E-52	-1.69E-46
MS.gene055891	MS.gene39518	0.810328	1.22E-50	-1.69E-46
MS.gene056043	MS.gene39518	-0.805433	1.34E-49	-1.69E-46
MS.gene056169	MS.gene39518	0.824867	6.40E-54	-1.69E-46
MS.gene056507	MS.gene39518	0.800168	1.64E-48	-1.69E-46
MS.gene057294	MS.gene39518	0.802681	5.00E-49	-1.69E-46
MS.gene058339	MS.gene39518	0.80851	2.99E-50	-1.69E-46
MS.gene058625	MS.gene39518	0.803731	3.03E-49	-1.69E-46
MS.gene058668	MS.gene39518	0.826519	2.60E-54	-1.69E-46
MS.gene05894	MS.gene39518	0.820249	7.58E-53	-1.69E-46
MS.gene059015	MS.gene39518	0.812417	4.28E-51	-1.69E-46
MS.gene059625	MS.gene39518	0.80067	1.29E-48	-1.69E-46
MS.gene060519	MS.gene39518	0.835642	1.49E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene39518.t1	MTR_4g088385	87.812	361	39	4	1	359	1	358	1.39e-144	413
MS.gene39518.t1	MTR_2g014400	45.238	168	63	3	191	358	122	260	1.55e-16	79.0
MS.gene39518.t1	MTR_2g014400	57.317	82	30	3	1	80	1	79	1.37e-13	70.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 44 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCGAGTTATATCCTTCTTC+TGG	0.161454	4.2:+22899638	None:intergenic
TGTTGTTCTGGTTTGTTTAA+TGG	0.166365	4.2:+22899983	None:intergenic
GACTGTCTCTAGCATGAATT+TGG	0.231861	4.2:+22900074	None:intergenic
CTTCAAACTGCTCTTGATTC+TGG	0.250818	4.2:+22899102	None:intergenic
TTCAAGGCTCAAATTCTTCT+TGG	0.258570	4.2:+22899075	None:intergenic
ATGAAAACTGGTTGTTGTTC+TGG	0.261146	4.2:+22899971	None:intergenic
TTTAATGGTGTTTCATTGTT+AGG	0.261763	4.2:+22899998	None:intergenic
CAAGTATTCTTCTAGTTCTA+AGG	0.327940	4.2:-22899681	MS.gene39518:CDS
TTGATTCTGGTAACCATTCA+TGG	0.329924	4.2:+22899115	None:intergenic
GAAAACAGTTATGGTTTATA+TGG	0.334786	4.2:-22899938	MS.gene39518:CDS
GGTTTATATGGTCATGATAC+TGG	0.336006	4.2:-22899926	MS.gene39518:CDS
ATTGAGTTGTAACCTTCTTC+TGG	0.340437	4.2:+22899437	None:intergenic
TTCATTCGGGTACTTGTTAC+CGG	0.356391	4.2:+22899772	None:intergenic
ATCAACATTGTTTGTGTTAG+TGG	0.360634	4.2:+22899886	None:intergenic
TCGGTTTCTGGGATGAAAAC+TGG	0.367232	4.2:+22899959	None:intergenic
TTCAACTACCAGCAAGATTC+CGG	0.377221	4.2:-22899791	MS.gene39518:CDS
TTCATATGATTGGAAAGTGT+TGG	0.393181	4.2:+22899862	None:intergenic
GTTAGTGACACAAAACTAAC+AGG	0.409476	4.2:-22899749	MS.gene39518:CDS
ACTTGTTACCGGAATCTTGC+TGG	0.411805	4.2:+22899783	None:intergenic
TTTGTGTCACTAACTTCATT+CGG	0.412326	4.2:+22899758	None:intergenic
CCAGAAACCGAAAACAGTTA+TGG	0.412492	4.2:-22899947	MS.gene39518:CDS
TCGAGTTATATCCTTCTTCT+GGG	0.415804	4.2:+22899639	None:intergenic
TCATATGATTGGAAAGTGTT+GGG	0.422874	4.2:+22899863	None:intergenic
TTGTGTCACTAACTTCATTC+GGG	0.424480	4.2:+22899759	None:intergenic
TTGAGTTGTAACCTTCTTCT+GGG	0.440468	4.2:+22899438	None:intergenic
ATTGTTTGTGTTAGTGGTAG+AGG	0.443186	4.2:+22899892	None:intergenic
AACACAAAGTTTACAGAAGA+AGG	0.451910	4.2:-22899542	MS.gene39518:CDS
TTCAATGGAGATTCATCTAA+AGG	0.457617	4.2:-22899221	MS.gene39518:CDS
AATGAGTGACACAAGAGTTA+TGG	0.465302	4.2:-22899297	MS.gene39518:CDS
AAATATAATCCAACATTCAA+TGG	0.466065	4.2:-22899236	MS.gene39518:CDS
AACAGAAAGTTCCCAGAAGA+AGG	0.473285	4.2:-22899449	MS.gene39518:CDS
GAGTTGTGAACTCTGAGAAT+TGG	0.491359	4.2:-22899199	MS.gene39518:CDS
GATGAATCTCCATTGAATGT+TGG	0.492045	4.2:+22899227	None:intergenic
TTGTTTGTGTTAGTGGTAGA+GGG	0.528399	4.2:+22899893	None:intergenic
GAATTTGGAGAGAAAATGAA+AGG	0.529100	4.2:+22900089	None:intergenic
AATAACAAGAACTCCATGAA+TGG	0.530258	4.2:-22899128	MS.gene39518:CDS
TTGTTAAGAATAGAAAAGAA+AGG	0.551144	4.2:+22900113	None:intergenic
AATTTGGAGAGAAAATGAAA+GGG	0.593601	4.2:+22900090	None:intergenic
TTTGTGTTAGTGGTAGAGGG+AGG	0.600533	4.2:+22899896	None:intergenic
AGAAGAAGGATACAACTCCA+TGG	0.616572	4.2:-22899528	MS.gene39518:CDS
AGTAACAACAATTACAACAA+TGG	0.621284	4.2:-22899332	MS.gene39518:CDS
TACAACAATGGTGAGAGACA+AGG	0.624719	4.2:-22899320	MS.gene39518:CDS
GACACAAGAGTTATGGAAGG+TGG	0.628338	4.2:-22899290	MS.gene39518:CDS
AGTGACACAAGAGTTATGGA+AGG	0.692016	4.2:-22899293	MS.gene39518:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATATAATCCAACATTCAA+TGG	-	chr4.2:22899973-22899992	MS.gene39518:CDS	20.0%
!!	TTGTTAAGAATAGAAAAGAA+AGG	+	chr4.2:22899099-22899118	None:intergenic	20.0%
!!!	ATATAAACCATAACTGTTTT+CGG	+	chr4.2:22899272-22899291	None:intergenic	20.0%
!!!	ATGAAGTTTTTTCATATGAT+TGG	+	chr4.2:22899360-22899379	None:intergenic	20.0%
!	AATTTGGAGAGAAAATGAAA+GGG	+	chr4.2:22899122-22899141	None:intergenic	25.0%
!	AGTAACAACAATTACAACAA+TGG	-	chr4.2:22899877-22899896	MS.gene39518:CDS	25.0%
!	GAAAACAGTTATGGTTTATA+TGG	-	chr4.2:22899271-22899290	MS.gene39518:CDS	25.0%
!	TACAACAAAAAAGGTTACTT+TGG	-	chr4.2:22900033-22900052	MS.gene39518:CDS	25.0%
!	TTTAATGGTGTTTCATTGTT+AGG	+	chr4.2:22899214-22899233	None:intergenic	25.0%
!!	TTTTTATGATGTTAACTCTG+AGG	-	chr4.2:22899948-22899967	MS.gene39518:CDS	25.0%
!!!	AAAAGAAAGGAATTTTGAGA+TGG	+	chr4.2:22899086-22899105	None:intergenic	25.0%
!!!	AAAGAAAGGAATTTTGAGAT+GGG	+	chr4.2:22899085-22899104	None:intergenic	25.0%
	AACACAAAGTTTACAGAAGA+AGG	-	chr4.2:22899667-22899686	MS.gene39518:CDS	30.0%
	AATAACAAGAACTCCATGAA+TGG	-	chr4.2:22900081-22900100	MS.gene39518:CDS	30.0%
	ATCAACATTGTTTGTGTTAG+TGG	+	chr4.2:22899326-22899345	None:intergenic	30.0%
	GAATTTGGAGAGAAAATGAA+AGG	+	chr4.2:22899123-22899142	None:intergenic	30.0%
	GAGAATTGGTACAACAAAAA+AGG	-	chr4.2:22900024-22900043	MS.gene39518:CDS	30.0%
	TTCAATGGAGATTCATCTAA+AGG	-	chr4.2:22899988-22900007	MS.gene39518:CDS	30.0%
	TTTCTACAACAACAACAACA+AGG	-	chr4.2:22899633-22899652	MS.gene39518:CDS	30.0%
!	TTCATATGATTGGAAAGTGT+TGG	+	chr4.2:22899350-22899369	None:intergenic	30.0%
!	TTTGTGTCACTAACTTCATT+CGG	+	chr4.2:22899454-22899473	None:intergenic	30.0%
!!	CAAGTATTCTTCTAGTTCTA+AGG	-	chr4.2:22899528-22899547	MS.gene39518:CDS	30.0%
!!	TCATATGATTGGAAAGTGTT+GGG	+	chr4.2:22899349-22899368	None:intergenic	30.0%
!!	TGTTGTTCTGGTTTGTTTAA+TGG	+	chr4.2:22899229-22899248	None:intergenic	30.0%
	AACAAAAAGTTCCCAGAAGA+AGG	-	chr4.2:22899559-22899578	MS.gene39518:CDS	35.0%
	AATGAGTGACACAAGAGTTA+TGG	-	chr4.2:22899912-22899931	MS.gene39518:CDS	35.0%
	ATTGTTTGTGTTAGTGGTAG+AGG	+	chr4.2:22899320-22899339	None:intergenic	35.0%
	GATGAATCTCCATTGAATGT+TGG	+	chr4.2:22899985-22900004	None:intergenic	35.0%
	GGTTTATATGGTCATGATAC+TGG	-	chr4.2:22899283-22899302	MS.gene39518:CDS	35.0%
	GTTAGTGACACAAAACTAAC+AGG	-	chr4.2:22899460-22899479	MS.gene39518:CDS	35.0%
	TCGAGTTATATCCTTCTTCT+GGG	+	chr4.2:22899573-22899592	None:intergenic	35.0%
	TTGATTCTGGTAACCATTCA+TGG	+	chr4.2:22900097-22900116	None:intergenic	35.0%
	TTGTTTGTGTTAGTGGTAGA+GGG	+	chr4.2:22899319-22899338	None:intergenic	35.0%
!	ATGAAAACTGGTTGTTGTTC+TGG	+	chr4.2:22899241-22899260	None:intergenic	35.0%
!	ATTGAGTTGTAACCTTCTTC+TGG	+	chr4.2:22899775-22899794	None:intergenic	35.0%
!	CATAACTGTTTTCGGTTTCT+GGG	+	chr4.2:22899264-22899283	None:intergenic	35.0%
!	TTCGAGTTATATCCTTCTTC+TGG	+	chr4.2:22899574-22899593	None:intergenic	35.0%
!	TTGAGTTGTAACCTTCTTCT+GGG	+	chr4.2:22899774-22899793	None:intergenic	35.0%
!	TTGTGTCACTAACTTCATTC+GGG	+	chr4.2:22899453-22899472	None:intergenic	35.0%
!!	ACTTGTTGTTGTTGTTGTTC+TGG	+	chr4.2:22899711-22899730	None:intergenic	35.0%
	AACAGAAAGTTCCCAGAAGA+AGG	-	chr4.2:22899760-22899779	MS.gene39518:CDS	40.0%
	AGAAGAAGGATACAACTCCA+TGG	-	chr4.2:22899681-22899700	MS.gene39518:CDS	40.0%
	AGTGACACAAGAGTTATGGA+AGG	-	chr4.2:22899916-22899935	MS.gene39518:CDS	40.0%
	CCAGAAACCGAAAACAGTTA+TGG	-	chr4.2:22899262-22899281	MS.gene39518:CDS	40.0%
	CTTCAAACTGCTCTTGATTC+TGG	+	chr4.2:22900110-22900129	None:intergenic	40.0%
	GACTGTCTCTAGCATGAATT+TGG	+	chr4.2:22899138-22899157	None:intergenic	40.0%
	TACAACAATGGTGAGAGACA+AGG	-	chr4.2:22899889-22899908	MS.gene39518:CDS	40.0%
	TTCAACTACCAGCAAGATTC+CGG	-	chr4.2:22899418-22899437	MS.gene39518:CDS	40.0%
!	CCATAACTGTTTTCGGTTTC+TGG	+	chr4.2:22899265-22899284	None:intergenic	40.0%
!	GAGTTGTGAACTCTGAGAAT+TGG	-	chr4.2:22900010-22900029	MS.gene39518:CDS	40.0%
!	TTCATTCGGGTACTTGTTAC+CGG	+	chr4.2:22899440-22899459	None:intergenic	40.0%
!!!	GTTGTTGTTCTGGTTTTCCA+TGG	+	chr4.2:22899701-22899720	None:intergenic	40.0%
	GACACAAGAGTTATGGAAGG+TGG	-	chr4.2:22899919-22899938	MS.gene39518:CDS	45.0%
	TTTGTGTTAGTGGTAGAGGG+AGG	+	chr4.2:22899316-22899335	None:intergenic	45.0%
!	ACTTGTTACCGGAATCTTGC+TGG	+	chr4.2:22899429-22899448	None:intergenic	45.0%
!!	TCGGTTTCTGGGATGAAAAC+TGG	+	chr4.2:22899253-22899272	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	22899076	22900155	22899076	ID=MS.gene39518
chr4.2	mRNA	22899076	22900155	22899076	ID=MS.gene39518.t1;Parent=MS.gene39518
chr4.2	exon	22899076	22900155	22899076	ID=MS.gene39518.t1.exon1;Parent=MS.gene39518.t1
chr4.2	CDS	22899076	22900155	22899076	ID=cds.MS.gene39518.t1;Parent=MS.gene39518.t1

Gene Sequence

>MS.gene39518

ATGGCTCCCATCTCAAAATTCCTTTCTTTTCTATTCTTAACAACCCTTTCATTTTCTCTCCAAATTCATGCTAGAGACAGTCAATTCTTCAGCAAAGTCACTCATGTCAACAACAACAACAATGTTAGAGAAATTCCTAACAATGAAACACCATTAAACAAACCAGAACAACAACCAGTTTTCATCCCAGAAACCGAAAACAGTTATGGTTTATATGGTCATGATACTGGTCTACATCCTCCCTCTACCACTAACACAAACAATGTTGATCCCAACACTTTCCAATCATATGAAAAAACTTCATCACACGATGAAATCAACAACAACAAAGTTAACAATTACTTCAACTACCAGCAAGATTCCGGTAACAAGTACCCGAATGAAGTTAGTGACACAAAACTAACAGGAACATCTTACACAAACAACAACAACAACTACAACTACGACAACAACAAGTATTCTTCTAGTTCTAAGGACTTTAGCAACAAAAAGTTCCCAGAAGAAGGATATAACTCGAACGAAAATCAGAACAACAACAACAACAACGATAACAAGTATTTCTACAACAACAACAACAAGGATTCTTCTAGCAACACAAAGTTTACAGAAGAAGGATACAACTCCATGGAAAACCAGAACAACAACAACAACAAGTATTTCTACAACAACAAAGATGCTTCTAGCAACAGAAAGTTCCCAGAAGAAGGTTACAACTCAATGCAAAATCAGAACGACAACAACAACTACGAGAAGTATAACTACAACAACAAAGTTGCTGTTAATGACAAATACAACTTCAAAAGTAACAACAATTACAACAATGGTGAGAGACAAGGAATGAGTGACACAAGAGTTATGGAAGGTGGAAAATATTTTTATGATGTTAACTCTGAGGAGAAATATAATCCAACATTCAATGGAGATTCATCTAAAGGAGTTGTGAACTCTGAGAATTGGTACAACAAAAAAGGTTACTTTGGTAACAACAATGTGAACAACTATGAGAATAACAAGAACTCCATGAATGGTTACCAGAATCAAGAGCAGTTTGAAGATGACCAAGAAGAATTTGAGCCTTGA

Protein sequence

>MS.gene39518.t1

MAPISKFLSFLFLTTLSFSLQIHARDSQFFSKVTHVNNNNNVREIPNNETPLNKPEQQPVFIPETENSYGLYGHDTGLHPPSTTNTNNVDPNTFQSYEKTSSHDEINNNKVNNYFNYQQDSGNKYPNEVSDTKLTGTSYTNNNNNYNYDNNKYSSSSKDFSNKKFPEEGYNSNENQNNNNNNDNKYFYNNNNKDSSSNTKFTEEGYNSMENQNNNNNKYFYNNKDASSNRKFPEEGYNSMQNQNDNNNYEKYNYNNKVAVNDKYNFKSNNNYNNGERQGMSDTRVMEGGKYFYDVNSEEKYNPTFNGDSSKGVVNSENWYNKKGYFGNNNVNNYENNKNSMNGYQNQEQFEDDQEEFEP