AlfalfaGEDB | MS.gene40871

M. sativa cultivar XinJiangDaYe / MS.gene40871

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005506 GO:0008150 GO:0008152 GO:0016491 GO:0016705 GO:0020037 GO:0043167 GO:0043169 GO:0046872 GO:0046906 GO:0046914 GO:0048037 GO:0055114 GO:0097159 GO:1901363
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40871.t1	XP_003614135.1	97.5	122	3	0	1	122	109	230	5.90E-63	250

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40871.t1	Q9LMX7	72.2	126	31	2	1	122	100	225	6.8e-44	177.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene40871.t1	G7JZ14	97.5	122	3	0	1	122	109	230	4.2e-63	250.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene40871	0.816506	5.34E-52	-1.69E-46
MS.gene049350	MS.gene40871	0.825483	4.57E-54	-1.69E-46
MS.gene049711	MS.gene40871	0.840932	6.46E-58	-1.69E-46
MS.gene049807	MS.gene40871	0.809198	2.13E-50	-1.69E-46
MS.gene051201	MS.gene40871	0.807776	4.28E-50	-1.69E-46
MS.gene051279	MS.gene40871	0.811402	7.13E-51	-1.69E-46
MS.gene051719	MS.gene40871	0.855623	5.57E-62	-1.69E-46
MS.gene051720	MS.gene40871	0.844229	8.60E-59	-1.69E-46
MS.gene052463	MS.gene40871	0.8347	2.58E-56	-1.69E-46
MS.gene052516	MS.gene40871	0.851098	1.11E-60	-1.69E-46
MS.gene053144	MS.gene40871	0.830742	2.47E-55	-1.69E-46
MS.gene054584	MS.gene40871	0.823695	1.21E-53	-1.69E-46
MS.gene054710	MS.gene40871	0.848805	4.85E-60	-1.69E-46
MS.gene055083	MS.gene40871	0.806714	7.19E-50	-1.69E-46
MS.gene055144	MS.gene40871	0.80061	1.33E-48	-1.69E-46
MS.gene055465	MS.gene40871	0.848848	4.72E-60	-1.69E-46
MS.gene055466	MS.gene40871	0.828583	8.29E-55	-1.69E-46
MS.gene055467	MS.gene40871	0.806163	9.40E-50	-1.69E-46
MS.gene055468	MS.gene40871	0.818695	1.71E-52	-1.69E-46
MS.gene055469	MS.gene40871	0.807479	4.95E-50	-1.69E-46
MS.gene055537	MS.gene40871	0.816673	4.90E-52	-1.69E-46
MS.gene055580	MS.gene40871	-0.802352	5.84E-49	-1.69E-46
MS.gene05574	MS.gene40871	0.810641	1.04E-50	-1.69E-46
MS.gene055891	MS.gene40871	0.801196	1.01E-48	-1.69E-46
MS.gene05604	MS.gene40871	0.816039	6.79E-52	-1.69E-46
MS.gene056308	MS.gene40871	0.825113	5.59E-54	-1.69E-46
MS.gene058087	MS.gene40871	0.820116	8.13E-53	-1.69E-46
MS.gene058625	MS.gene40871	0.824171	9.33E-54	-1.69E-46
MS.gene060122	MS.gene40871	-0.809711	1.65E-50	-1.69E-46
MS.gene060519	MS.gene40871	0.8092	2.13E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 26 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAATCTCTTTAGCAGTTTC+TGG	0.158283	5.1:-38015567	None:intergenic
CTGATATGATTGCAGTTCTT+TGG	0.226158	5.1:+38016171	MS.gene40871:intron
ATTCTTTGTAGTTCTGCTTT+TGG	0.287687	5.1:+38015585	MS.gene40871:CDS
TGATATGATTGCAGTTCTTT+GGG	0.349760	5.1:+38016172	MS.gene40871:intron
GAGTGAGGTTGGTTTGAAAA+TGG	0.370170	5.1:+38015758	MS.gene40871:CDS
AAATGTTATAATTTCTTTGA+TGG	0.372840	5.1:+38015882	MS.gene40871:CDS
TCCTCCTAGGACAAAACAAA+TGG	0.409716	5.1:-38015713	None:intergenic
GCAATGGGGTTTGCTCCATA+TGG	0.440904	5.1:+38015654	MS.gene40871:CDS
AATGTGATGATGACTGTGTT+TGG	0.462446	5.1:+38015858	MS.gene40871:CDS
AGGAGGATTAACGGGTTTGA+AGG	0.463080	5.1:+38015729	MS.gene40871:CDS
TTCCTCCAGTACTCTCCATA+TGG	0.464280	5.1:-38015669	None:intergenic
TGGTGTTGAGCTTGAGGAGT+TGG	0.466004	5.1:+38015908	MS.gene40871:CDS
TATAATTTCTTTGATGGTGA+TGG	0.491539	5.1:+38015888	MS.gene40871:CDS
TGGTGATGGTGTTGAGCTTG+AGG	0.495676	5.1:+38015902	MS.gene40871:CDS
TTTGTCCTAGGAGGATTAAC+GGG	0.498945	5.1:+38015721	MS.gene40871:CDS
CTCGTAAGCTGACTCTTTCA+CGG	0.501144	5.1:-38015616	None:intergenic
TTGCTCCATATGGAGAGTAC+TGG	0.501285	5.1:+38015664	MS.gene40871:CDS
CCTCCTAGGACAAAACAAAT+GGG	0.538810	5.1:-38015712	None:intergenic
TGAGGTTGGTTTGAAAATGG+TGG	0.559394	5.1:+38015761	MS.gene40871:CDS
TCGTAAGCTGACTCTTTCAC+GGG	0.580639	5.1:-38015615	None:intergenic
TCAAACCCGTTAATCCTCCT+AGG	0.581092	5.1:-38015726	None:intergenic
CTCCATATGGAGAGTACTGG+AGG	0.596336	5.1:+38015667	MS.gene40871:CDS
GAGAGTACTGGAGGAATCTG+AGG	0.600190	5.1:+38015676	MS.gene40871:CDS
TTGGTTTGAAAATGGTGGAG+AGG	0.606844	5.1:+38015766	MS.gene40871:CDS
GGGTGATATGGGTAGTGTTG+AGG	0.612456	5.1:+38015803	MS.gene40871:CDS
AGTACTGGAGGAATCTGAGG+AGG	0.670852	5.1:+38015679	MS.gene40871:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATGTTATAATTTCTTTGA+TGG	+	chr5.1:38015882-38015901	MS.gene40871:CDS	15.0%
!!	TTTTGTTGGAAAGATAATAG+AGG	+	chr5.1:38016052-38016071	MS.gene40871:intron	25.0%
!!!	TATAATTTCTTTGATGGTGA+TGG	+	chr5.1:38015888-38015907	MS.gene40871:CDS	25.0%
	TGATATGATTGCAGTTCTTT+GGG	+	chr5.1:38016172-38016191	MS.gene40871:intron	30.0%
!	ACAAAGGTTAATGCTTTTGT+TGG	+	chr5.1:38016038-38016057	MS.gene40871:intron	30.0%
!	AGTGATCATTTTCCTCTTTT+GGG	+	chr5.1:38015969-38015988	MS.gene40871:intron	30.0%
!!	ATTCTTTGTAGTTCTGCTTT+TGG	+	chr5.1:38015585-38015604	MS.gene40871:CDS	30.0%
!!	TTACGAGCTTTTGTTTCATA+GGG	+	chr5.1:38015632-38015651	MS.gene40871:CDS	30.0%
!!!	GTGAAAAAAGTGCTTCATTT+TGG	+	chr5.1:38015825-38015844	MS.gene40871:CDS	30.0%
	AAGATGAAGAGAATCTCTGA+AGG	+	chr5.1:38016080-38016099	MS.gene40871:intron	35.0%
	AGAATCTCTTTAGCAGTTTC+TGG	-	chr5.1:38015570-38015589	None:intergenic	35.0%
	CTGATATGATTGCAGTTCTT+TGG	+	chr5.1:38016171-38016190	MS.gene40871:intron	35.0%
!	AATGTGATGATGACTGTGTT+TGG	+	chr5.1:38015858-38015877	MS.gene40871:CDS	35.0%
!	GAGTGATCATTTTCCTCTTT+TGG	+	chr5.1:38015968-38015987	MS.gene40871:intron	35.0%
!	TGTTGACGTTTTGCTTGATT+TGG	+	chr5.1:38016124-38016143	MS.gene40871:intron	35.0%
!	TTTTGTCCTAGGAGGATTAA+CGG	+	chr5.1:38015720-38015739	MS.gene40871:CDS	35.0%
!!	ATCATTTTCCTCTTTTGGGT+TGG	+	chr5.1:38015973-38015992	MS.gene40871:intron	35.0%
!!	CTTACGAGCTTTTGTTTCAT+AGG	+	chr5.1:38015631-38015650	MS.gene40871:CDS	35.0%
!!	CTTTTGTTTCATAGGGCAAT+GGG	+	chr5.1:38015639-38015658	MS.gene40871:CDS	35.0%
!!	TTTTGTTTCATAGGGCAATG+GGG	+	chr5.1:38015640-38015659	MS.gene40871:CDS	35.0%
!!!	TTAACGGGTTTGAAGGTTTT+AGG	+	chr5.1:38015736-38015755	MS.gene40871:CDS	35.0%
	AAATCCAACCAACCCAAAAG+AGG	-	chr5.1:38015984-38016003	None:intergenic	40.0%
	CCTCCTAGGACAAAACAAAT+GGG	-	chr5.1:38015715-38015734	None:intergenic	40.0%
	TCCTCCTAGGACAAAACAAA+TGG	-	chr5.1:38015716-38015735	None:intergenic	40.0%
	TCTGAAGGAAAGGCTATAGT+TGG	+	chr5.1:38016095-38016114	MS.gene40871:intron	40.0%
	TTTGTCCTAGGAGGATTAAC+GGG	+	chr5.1:38015721-38015740	MS.gene40871:CDS	40.0%
!	ATTTGCAGGGTGTTAGGAAA+AGG	+	chr5.1:38016000-38016019	MS.gene40871:intron	40.0%
!	GAAGAGAATCTCTGAAGGAA+AGG	+	chr5.1:38016085-38016104	MS.gene40871:intron	40.0%
!	GAGAGGATAGAGCTTTTGAT+GGG	+	chr5.1:38015783-38015802	MS.gene40871:CDS	40.0%
!	TAGGAAAAGGTGTAGAGCTT+TGG	+	chr5.1:38016013-38016032	MS.gene40871:intron	40.0%
!	TTGGTTTGAAAATGGTGGAG+AGG	+	chr5.1:38015766-38015785	MS.gene40871:CDS	40.0%
!!	AGAGCTTTTGATGGGTGATA+TGG	+	chr5.1:38015791-38015810	MS.gene40871:CDS	40.0%
!!	AGCTGTTGGGTGTTTTTAAC+TGG	+	chr5.1:38015946-38015965	MS.gene40871:intron	40.0%
!!	GAGCTTTTGATGGGTGATAT+GGG	+	chr5.1:38015792-38015811	MS.gene40871:CDS	40.0%
!!	GAGTGAGGTTGGTTTGAAAA+TGG	+	chr5.1:38015758-38015777	MS.gene40871:CDS	40.0%
!!	GCTTTTGTTTCATAGGGCAA+TGG	+	chr5.1:38015638-38015657	MS.gene40871:CDS	40.0%
!!	TGAGGTTGGTTTGAAAATGG+TGG	+	chr5.1:38015761-38015780	MS.gene40871:CDS	40.0%
!!!	CAACCCATTTGTTTTGTCCT+AGG	+	chr5.1:38015709-38015728	MS.gene40871:CDS	40.0%
!!!	GACGTTTTGCTTGATTTGGA+GGG	+	chr5.1:38016128-38016147	MS.gene40871:intron	40.0%
!!!	GTTTGAAGGTTTTAGGAGTG+AGG	+	chr5.1:38015743-38015762	MS.gene40871:CDS	40.0%
!!!	TGACGTTTTGCTTGATTTGG+AGG	+	chr5.1:38016127-38016146	MS.gene40871:intron	40.0%
!!!	TTTTCCTCTTTTGGGTTGGT+TGG	+	chr5.1:38015977-38015996	MS.gene40871:intron	40.0%
	TCAAACCCGTTAATCCTCCT+AGG	-	chr5.1:38015729-38015748	None:intergenic	45.0%
	TTCCTCCAGTACTCTCCATA+TGG	-	chr5.1:38015672-38015691	None:intergenic	45.0%
!	AGTGAAGGGTATGAGCTGTT+GGG	+	chr5.1:38015933-38015952	MS.gene40871:intron	45.0%
!	CTCGTAAGCTGACTCTTTCA+CGG	-	chr5.1:38015619-38015638	None:intergenic	45.0%
!	GGAGAGGATAGAGCTTTTGA+TGG	+	chr5.1:38015782-38015801	MS.gene40871:CDS	45.0%
!	GTGTAGAGCTTTGGTGACAA+AGG	+	chr5.1:38016022-38016041	MS.gene40871:intron	45.0%
!	TCGTAAGCTGACTCTTTCAC+GGG	-	chr5.1:38015618-38015637	None:intergenic	45.0%
!	TTGCTCCATATGGAGAGTAC+TGG	+	chr5.1:38015664-38015683	MS.gene40871:CDS	45.0%
!!	AGGAGGATTAACGGGTTTGA+AGG	+	chr5.1:38015729-38015748	MS.gene40871:CDS	45.0%
!!	TTGAGGAGTTGGTGAGTGAA+GGG	+	chr5.1:38015919-38015938	MS.gene40871:intron	45.0%
!!	TTGGGTTGGTTGGATTTGCA+GGG	+	chr5.1:38015987-38016006	MS.gene40871:intron	45.0%
!!	TTTGGGTTGGTTGGATTTGC+AGG	+	chr5.1:38015986-38016005	MS.gene40871:intron	45.0%
!!!	CCCATTTGTTTTGTCCTAGG+AGG	+	chr5.1:38015712-38015731	MS.gene40871:CDS	45.0%
!!!	GAAGGTTTTAGGAGTGAGGT+TGG	+	chr5.1:38015747-38015766	MS.gene40871:CDS	45.0%
	GGGTGATATGGGTAGTGTTG+AGG	+	chr5.1:38015803-38015822	MS.gene40871:CDS	50.0%
!	AGTACTGGAGGAATCTGAGG+AGG	+	chr5.1:38015679-38015698	MS.gene40871:CDS	50.0%
!	GAGTGAAGGGTATGAGCTGT+TGG	+	chr5.1:38015932-38015951	MS.gene40871:intron	50.0%
!	GCAATGGGGTTTGCTCCATA+TGG	+	chr5.1:38015654-38015673	MS.gene40871:CDS	50.0%
!	GGTTGGATTTGCAGGGTGTT+AGG	+	chr5.1:38015994-38016013	MS.gene40871:intron	50.0%
!	TGGTGTTGAGCTTGAGGAGT+TGG	+	chr5.1:38015908-38015927	MS.gene40871:CDS	50.0%
!!	CTCCATATGGAGAGTACTGG+AGG	+	chr5.1:38015667-38015686	MS.gene40871:CDS	50.0%
!!	CTTGAGGAGTTGGTGAGTGA+AGG	+	chr5.1:38015918-38015937	MS.gene40871:intron	50.0%
!!	GAGAGTACTGGAGGAATCTG+AGG	+	chr5.1:38015676-38015695	MS.gene40871:CDS	50.0%
!!	TGGTGATGGTGTTGAGCTTG+AGG	+	chr5.1:38015902-38015921	MS.gene40871:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	38015563	38016197	38015563	ID=MS.gene40871
chr5.1	mRNA	38015563	38016197	38015563	ID=MS.gene40871.t1;Parent=MS.gene40871
chr5.1	exon	38015563	38015929	38015563	ID=MS.gene40871.t1.exon1;Parent=MS.gene40871.t1
chr5.1	CDS	38015563	38015929	38015563	ID=cds.MS.gene40871.t1;Parent=MS.gene40871.t1
chr5.1	exon	38016186	38016197	38016186	ID=MS.gene40871.t1.exon2;Parent=MS.gene40871.t1
chr5.1	CDS	38016186	38016197	38016186	ID=cds.MS.gene40871.t1;Parent=MS.gene40871.t1

Gene Sequence

>MS.gene40871

TGAACCAGAAACTGCTAAAGAGATTCTTTGTAGTTCTGCTTTTGGTGATAGACCCGTGAAAGAGTCAGCTTACGAGCTTTTGTTTCATAGGGCAATGGGGTTTGCTCCATATGGAGAGTACTGGAGGAATCTGAGGAGGATTTCTTCAACCCATTTGTTTTGTCCTAGGAGGATTAACGGGTTTGAAGGTTTTAGGAGTGAGGTTGGTTTGAAAATGGTGGAGAGGATAGAGCTTTTGATGGGTGATATGGGTAGTGTTGAGGTGAAAAAAGTGCTTCATTTTGGATCACTTAATAATGTGATGATGACTGTGTTTGGAAAATGTTATAATTTCTTTGATGGTGATGGTGTTGAGCTTGAGGAGTTGGTGAGTGAAGGGTATGAGCTGTTGGGTGTTTTTAACTGGAGTGATCATTTTCCTCTTTTGGGTTGGTTGGATTTGCAGGGTGTTAGGAAAAGGTGTAGAGCTTTGGTGACAAAGGTTAATGCTTTTGTTGGAAAGATAATAGAGGAGCATAAGATGAAGAGAATCTCTGAAGGAAAGGCTATAGTTGGTGATTTTGTTGACGTTTTGCTTGATTTGGAGGGAAAAGACAAGCTCAGTGATTCTGATATGATTGCAGTTCTTTGGGTAA

Protein sequence

>MS.gene40871.t1

UTRNCURDSLUFCFWUUTRERVSLRAFVSUGNGVCSIWRVLEESEEDFFNPFVLSUEDURVURFUEUGWFENGGEDRAFDGUYGUCUGEKSASFWITUUCDDDCVWKMLUFLUWUWCUAUGVVLWV