Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05574.t1 | CAA76366.1 | 92.5 | 279 | 21 | 0 | 1 | 279 | 1 | 279 | 3.10E-141 | 511.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05574.t1 | Q01807 | 91.8 | 280 | 22 | 1 | 1 | 279 | 1 | 280 | 3.2e-142 | 505.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05574.t1 | O49899 | 92.5 | 279 | 21 | 0 | 1 | 279 | 1 | 279 | 2.3e-141 | 511.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050657 | MS.gene05574 | 0.8113 | 7.50E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene05574 | 0.80575 | 1.15E-49 | -1.69E-46 |
| MS.gene05573 | MS.gene05574 | 0.881574 | 2.14E-70 | -1.69E-46 |
| MS.gene05574 | MS.gene063149 | 0.801522 | 8.65E-49 | -1.69E-46 |
| MS.gene05574 | MS.gene066196 | 0.832522 | 9.01E-56 | -1.69E-46 |
| MS.gene05574 | MS.gene20457 | 0.800361 | 1.49E-48 | -1.69E-46 |
| MS.gene05574 | MS.gene21826 | 0.80771 | 4.43E-50 | -1.69E-46 |
| MS.gene05574 | MS.gene26659 | 0.805106 | 1.57E-49 | -1.69E-46 |
| MS.gene05574 | MS.gene34286 | 0.802103 | 6.58E-49 | -1.69E-46 |
| MS.gene05574 | MS.gene34453 | 0.800308 | 1.53E-48 | -1.69E-46 |
| MS.gene05574 | MS.gene38303 | 0.807371 | 5.22E-50 | -1.69E-46 |
| MS.gene05574 | MS.gene40871 | 0.810641 | 1.04E-50 | -1.69E-46 |
| MS.gene05574 | MS.gene45281 | 0.815987 | 6.98E-52 | -1.69E-46 |
| MS.gene05574 | MS.gene49467 | 0.826598 | 2.48E-54 | -1.69E-46 |
| MS.gene05574 | MS.gene51384 | 0.816013 | 6.88E-52 | -1.69E-46 |
| MS.gene05574 | MS.gene56510 | 0.811555 | 6.60E-51 | -1.69E-46 |
| MS.gene05574 | MS.gene56565 | 0.816181 | 6.31E-52 | -1.69E-46 |
| MS.gene05574 | MS.gene59241 | 0.804788 | 1.83E-49 | -1.69E-46 |
| MS.gene05574 | MS.gene59242 | 0.807524 | 4.85E-50 | -1.69E-46 |
| MS.gene05574 | MS.gene67193 | 0.830598 | 2.68E-55 | -1.69E-46 |
| MS.gene05574 | MS.gene75719 | 0.819442 | 1.16E-52 | -1.69E-46 |
| MS.gene05574 | MS.gene80732 | 0.812295 | 4.55E-51 | -1.69E-46 |
| MS.gene05574 | MS.gene88254 | 0.812575 | 3.96E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05574.t1 | MTR_3g463370 | 79.286 | 280 | 18 | 2 | 1 | 279 | 1 | 241 | 1.20e-152 | 426 |
| MS.gene05574.t1 | MTR_8g023510 | 70.677 | 266 | 68 | 4 | 1 | 263 | 3 | 261 | 2.47e-124 | 355 |
| MS.gene05574.t1 | MTR_4g023810 | 56.846 | 241 | 94 | 3 | 22 | 262 | 25 | 255 | 1.59e-92 | 275 |
| MS.gene05574.t1 | MTR_8g068040 | 37.736 | 265 | 140 | 7 | 9 | 261 | 10 | 261 | 1.10e-48 | 162 |
| MS.gene05574.t1 | MTR_5g031100 | 39.259 | 270 | 134 | 8 | 17 | 261 | 14 | 278 | 3.56e-48 | 161 |
| MS.gene05574.t1 | MTR_5g031120 | 39.502 | 281 | 138 | 9 | 7 | 261 | 5 | 279 | 5.38e-48 | 161 |
| MS.gene05574.t1 | MTR_8g068030 | 37.226 | 274 | 133 | 9 | 9 | 261 | 10 | 265 | 8.64e-48 | 160 |
| MS.gene05574.t1 | MTR_3g054170 | 40.909 | 264 | 143 | 5 | 1 | 261 | 1 | 254 | 1.09e-46 | 157 |
| MS.gene05574.t1 | MTR_5g031140 | 38.129 | 278 | 143 | 9 | 7 | 261 | 15 | 286 | 2.90e-45 | 154 |
| MS.gene05574.t1 | MTR_5g031160 | 38.889 | 252 | 136 | 8 | 23 | 261 | 41 | 287 | 2.91e-45 | 154 |
| MS.gene05574.t1 | MTR_3g054180 | 41.132 | 265 | 143 | 5 | 1 | 261 | 5 | 260 | 1.85e-44 | 151 |
| MS.gene05574.t1 | MTR_3g027280 | 39.922 | 258 | 130 | 9 | 12 | 257 | 18 | 262 | 6.15e-44 | 150 |
| MS.gene05574.t1 | MTR_3g047140 | 40.458 | 262 | 139 | 8 | 6 | 261 | 11 | 261 | 8.12e-44 | 150 |
| MS.gene05574.t1 | MTR_8g068050 | 38.060 | 268 | 144 | 7 | 10 | 264 | 18 | 276 | 2.95e-42 | 154 |
| MS.gene05574.t1 | MTR_5g031090 | 36.653 | 251 | 137 | 8 | 27 | 261 | 31 | 275 | 3.26e-41 | 143 |
| MS.gene05574.t1 | MTR_5g031030 | 36.400 | 250 | 135 | 8 | 27 | 261 | 34 | 274 | 9.39e-40 | 139 |
| MS.gene05574.t1 | MTR_1g090973 | 36.232 | 276 | 156 | 9 | 6 | 274 | 11 | 273 | 6.86e-39 | 137 |
| MS.gene05574.t1 | MTR_0163s0020 | 37.269 | 271 | 150 | 6 | 7 | 271 | 14 | 270 | 1.56e-38 | 136 |
| MS.gene05574.t1 | MTR_0684s0020 | 37.269 | 271 | 150 | 6 | 7 | 271 | 13 | 269 | 1.77e-38 | 136 |
| MS.gene05574.t1 | MTR_8g067940 | 35.766 | 274 | 158 | 9 | 3 | 261 | 11 | 281 | 3.14e-38 | 136 |
| MS.gene05574.t1 | MTR_8g067980 | 40.486 | 247 | 120 | 12 | 31 | 262 | 26 | 260 | 5.13e-38 | 135 |
| MS.gene05574.t1 | MTR_1g090957 | 37.452 | 259 | 123 | 10 | 12 | 257 | 18 | 250 | 9.44e-37 | 132 |
| MS.gene05574.t1 | MTR_8g067930 | 37.066 | 259 | 140 | 8 | 16 | 261 | 11 | 259 | 3.07e-36 | 136 |
| MS.gene05574.t1 | MTR_4g093080 | 35.587 | 281 | 154 | 10 | 8 | 276 | 9 | 274 | 1.06e-35 | 135 |
| MS.gene05574.t1 | MTR_8g067630 | 39.640 | 222 | 113 | 8 | 32 | 242 | 33 | 244 | 1.60e-34 | 131 |
| MS.gene05574.t1 | MTR_8g067720 | 31.734 | 271 | 161 | 7 | 1 | 261 | 1 | 257 | 1.92e-34 | 131 |
| MS.gene05574.t1 | MTR_8g067690 | 35.833 | 240 | 136 | 7 | 32 | 261 | 63 | 294 | 2.30e-34 | 131 |
| MS.gene05574.t1 | MTR_3g054200 | 36.364 | 264 | 155 | 5 | 1 | 261 | 1 | 254 | 4.99e-34 | 124 |
| MS.gene05574.t1 | MTR_4g093140 | 33.588 | 262 | 151 | 9 | 12 | 263 | 18 | 266 | 3.38e-33 | 128 |
| MS.gene05574.t1 | MTR_8g067990 | 37.860 | 243 | 126 | 11 | 31 | 259 | 26 | 257 | 4.25e-32 | 119 |
| MS.gene05574.t1 | MTR_1g090943 | 35.484 | 279 | 151 | 11 | 9 | 274 | 15 | 277 | 1.14e-31 | 119 |
| MS.gene05574.t1 | MTR_8g067960 | 36.287 | 237 | 128 | 9 | 37 | 261 | 16 | 241 | 1.84e-31 | 117 |
| MS.gene05574.t1 | MTR_8g067735 | 33.212 | 274 | 155 | 9 | 1 | 261 | 20 | 278 | 4.97e-31 | 122 |
| MS.gene05574.t1 | MTR_1g090930 | 36.882 | 263 | 135 | 10 | 8 | 256 | 13 | 258 | 1.19e-30 | 116 |
| MS.gene05574.t1 | MTR_1g090937 | 34.661 | 251 | 138 | 8 | 15 | 256 | 21 | 254 | 1.69e-30 | 115 |
| MS.gene05574.t1 | MTR_4g093110 | 31.939 | 263 | 154 | 10 | 12 | 263 | 18 | 266 | 1.40e-28 | 115 |
| MS.gene05574.t1 | MTR_1g014240 | 32.766 | 235 | 130 | 7 | 43 | 261 | 53 | 275 | 1.47e-28 | 115 |
| MS.gene05574.t1 | MTR_7g116130 | 37.500 | 240 | 114 | 12 | 43 | 262 | 39 | 262 | 6.59e-28 | 113 |
| MS.gene05574.t1 | MTR_4g093040 | 34.413 | 247 | 133 | 12 | 21 | 250 | 25 | 259 | 2.66e-27 | 111 |
| MS.gene05574.t1 | MTR_4g093070 | 33.036 | 224 | 116 | 6 | 58 | 263 | 61 | 268 | 1.66e-25 | 106 |
| MS.gene05574.t1 | MTR_4g093040 | 38.172 | 186 | 94 | 9 | 77 | 250 | 1 | 177 | 1.74e-25 | 105 |
| MS.gene05574.t1 | MTR_1g090960 | 31.786 | 280 | 133 | 9 | 6 | 274 | 11 | 243 | 6.44e-25 | 100 |
| MS.gene05574.t1 | MTR_7g115740 | 32.971 | 276 | 152 | 13 | 18 | 275 | 14 | 274 | 8.80e-25 | 103 |
| MS.gene05574.t1 | MTR_7g062940 | 29.924 | 264 | 156 | 7 | 12 | 257 | 3 | 255 | 1.49e-20 | 91.7 |
| MS.gene05574.t1 | MTR_1g048360 | 31.863 | 204 | 115 | 7 | 66 | 259 | 71 | 260 | 8.36e-20 | 89.4 |
| MS.gene05574.t1 | MTR_7g062890 | 29.588 | 267 | 150 | 8 | 14 | 257 | 5 | 256 | 2.78e-17 | 82.0 |
| MS.gene05574.t1 | MTR_5g025020 | 29.817 | 218 | 125 | 8 | 66 | 267 | 85 | 290 | 5.90e-17 | 80.9 |
| MS.gene05574.t1 | MTR_7g062950 | 28.302 | 265 | 159 | 8 | 12 | 257 | 3 | 255 | 9.01e-17 | 80.5 |
| MS.gene05574.t1 | MTR_5g035910 | 29.889 | 271 | 154 | 12 | 8 | 257 | 8 | 263 | 2.34e-16 | 79.3 |
| MS.gene05574.t1 | MTR_7g063030 | 28.571 | 259 | 155 | 8 | 16 | 257 | 15 | 260 | 4.28e-16 | 78.6 |
| MS.gene05574.t1 | MTR_4g056320 | 28.302 | 265 | 132 | 8 | 6 | 261 | 14 | 229 | 5.07e-16 | 76.3 |
| MS.gene05574.t1 | MTR_5g068770 | 31.308 | 214 | 117 | 9 | 64 | 259 | 76 | 277 | 7.74e-16 | 77.8 |
| MS.gene05574.t1 | MTR_7g062700 | 29.323 | 266 | 150 | 7 | 16 | 257 | 7 | 258 | 8.18e-16 | 77.8 |
| MS.gene05574.t1 | MTR_5g077100 | 30.542 | 203 | 119 | 5 | 69 | 264 | 68 | 255 | 1.09e-15 | 77.4 |
| MS.gene05574.t1 | MTR_7g062750 | 28.571 | 266 | 160 | 7 | 12 | 257 | 3 | 258 | 1.40e-15 | 77.0 |
| MS.gene05574.t1 | MTR_4g093050 | 33.010 | 206 | 109 | 9 | 67 | 257 | 71 | 262 | 1.51e-15 | 76.6 |
| MS.gene05574.t1 | MTR_1g052275 | 29.323 | 266 | 158 | 8 | 12 | 257 | 3 | 258 | 2.97e-15 | 75.9 |
| MS.gene05574.t1 | MTR_7g063030 | 29.310 | 232 | 138 | 7 | 43 | 257 | 32 | 254 | 4.86e-15 | 75.5 |
| MS.gene05574.t1 | MTR_7g063010 | 27.203 | 261 | 159 | 8 | 14 | 257 | 10 | 256 | 5.73e-15 | 75.1 |
| MS.gene05574.t1 | MTR_8g068050 | 29.348 | 184 | 116 | 4 | 10 | 186 | 18 | 194 | 1.11e-14 | 74.3 |
| MS.gene05574.t1 | MTR_7g062770 | 29.958 | 237 | 134 | 6 | 43 | 257 | 34 | 260 | 2.28e-13 | 70.5 |
| MS.gene05574.t1 | MTR_7g062990 | 26.515 | 264 | 164 | 7 | 14 | 257 | 6 | 259 | 2.69e-13 | 70.1 |
| MS.gene05574.t1 | MTR_7g062680 | 26.692 | 266 | 166 | 7 | 12 | 257 | 3 | 259 | 1.10e-12 | 68.2 |
| MS.gene05574.t1 | MTR_7g062900 | 26.695 | 236 | 134 | 7 | 43 | 257 | 34 | 251 | 1.58e-11 | 64.7 |
| MS.gene05574.t1 | MTR_7g115740 | 31.792 | 173 | 99 | 9 | 18 | 180 | 14 | 177 | 3.16e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene05574.t1 | AT5G10530 | 33.840 | 263 | 154 | 8 | 11 | 263 | 4 | 256 | 8.22e-29 | 115 |
| MS.gene05574.t1 | AT5G65600 | 33.478 | 230 | 137 | 6 | 32 | 250 | 38 | 262 | 3.37e-27 | 110 |
| MS.gene05574.t1 | AT4G02420 | 31.679 | 262 | 148 | 10 | 14 | 257 | 12 | 260 | 2.24e-20 | 91.3 |
| MS.gene05574.t1 | AT4G02410 | 31.621 | 253 | 148 | 7 | 25 | 260 | 22 | 266 | 4.72e-20 | 90.1 |
| MS.gene05574.t1 | AT2G37710 | 29.661 | 236 | 133 | 7 | 43 | 257 | 38 | 261 | 9.14e-20 | 89.4 |
| MS.gene05574.t1 | AT3G16530 | 27.957 | 279 | 155 | 11 | 14 | 259 | 6 | 271 | 1.99e-19 | 85.9 |
| MS.gene05574.t1 | AT2G29250 | 28.467 | 274 | 166 | 8 | 1 | 257 | 1 | 261 | 9.43e-19 | 86.3 |
| MS.gene05574.t1 | AT5G01560 | 30.078 | 256 | 140 | 10 | 32 | 259 | 30 | 274 | 1.22e-18 | 85.9 |
| MS.gene05574.t1 | AT3G53810 | 28.854 | 253 | 151 | 8 | 24 | 257 | 19 | 261 | 2.07e-18 | 85.5 |
| MS.gene05574.t1 | AT5G01540 | 29.183 | 257 | 148 | 9 | 29 | 259 | 30 | 278 | 5.12e-18 | 84.3 |
| MS.gene05574.t1 | AT5G60280 | 31.128 | 257 | 144 | 9 | 19 | 257 | 15 | 256 | 5.54e-18 | 84.0 |
| MS.gene05574.t1 | AT4G29050 | 31.381 | 239 | 138 | 7 | 48 | 269 | 53 | 282 | 8.50e-18 | 83.6 |
| MS.gene05574.t1 | AT4G29050 | 31.381 | 239 | 138 | 7 | 48 | 269 | 39 | 268 | 9.34e-18 | 83.6 |
| MS.gene05574.t1 | AT3G15356 | 29.365 | 252 | 130 | 10 | 43 | 258 | 31 | 270 | 1.12e-17 | 81.3 |
| MS.gene05574.t1 | AT3G55550 | 29.630 | 243 | 146 | 6 | 32 | 258 | 26 | 259 | 1.99e-17 | 82.4 |
| MS.gene05574.t1 | AT5G01550 | 26.786 | 280 | 172 | 8 | 6 | 259 | 18 | 290 | 9.20e-17 | 80.5 |
| MS.gene05574.t1 | AT3G45430 | 30.798 | 263 | 136 | 12 | 26 | 263 | 22 | 263 | 1.29e-16 | 80.1 |
| MS.gene05574.t1 | AT3G45430 | 30.798 | 263 | 136 | 12 | 26 | 263 | 22 | 263 | 1.29e-16 | 80.1 |
| MS.gene05574.t1 | AT3G45430 | 30.798 | 263 | 136 | 12 | 26 | 263 | 22 | 263 | 1.29e-16 | 80.1 |
| MS.gene05574.t1 | AT2G29220 | 27.341 | 267 | 165 | 8 | 10 | 258 | 7 | 262 | 9.15e-16 | 77.4 |
| MS.gene05574.t1 | AT5G55830 | 29.213 | 267 | 150 | 12 | 10 | 257 | 18 | 264 | 1.34e-15 | 77.0 |
| MS.gene05574.t1 | AT3G08870 | 26.070 | 257 | 161 | 8 | 26 | 259 | 30 | 280 | 8.59e-15 | 74.7 |
| MS.gene05574.t1 | AT3G08870 | 26.070 | 257 | 161 | 8 | 26 | 259 | 51 | 301 | 9.09e-15 | 74.7 |
| MS.gene05574.t1 | AT5G03140 | 29.358 | 218 | 133 | 7 | 48 | 255 | 51 | 257 | 1.17e-14 | 74.3 |
| MS.gene05574.t1 | AT5G60300 | 30.709 | 254 | 134 | 13 | 28 | 257 | 23 | 258 | 1.68e-14 | 73.9 |
| MS.gene05574.t1 | AT5G60300 | 30.709 | 254 | 134 | 13 | 28 | 257 | 23 | 258 | 1.68e-14 | 73.9 |
| MS.gene05574.t1 | AT5G60300 | 30.709 | 254 | 134 | 13 | 28 | 257 | 23 | 258 | 1.85e-14 | 73.6 |
| MS.gene05574.t1 | AT5G06740 | 31.220 | 205 | 118 | 9 | 64 | 258 | 78 | 269 | 9.91e-14 | 71.6 |
| MS.gene05574.t1 | AT5G06740 | 31.220 | 205 | 118 | 9 | 64 | 258 | 66 | 257 | 1.04e-13 | 71.2 |
| MS.gene05574.t1 | AT5G42120 | 30.037 | 273 | 143 | 10 | 6 | 257 | 11 | 256 | 5.38e-13 | 69.3 |
| MS.gene05574.t1 | AT3G45440 | 29.070 | 258 | 153 | 10 | 17 | 257 | 65 | 309 | 7.08e-13 | 68.9 |
| MS.gene05574.t1 | AT3G45440 | 29.070 | 258 | 153 | 10 | 17 | 257 | 12 | 256 | 8.28e-13 | 68.6 |
| MS.gene05574.t1 | AT3G45440 | 29.070 | 258 | 153 | 10 | 17 | 257 | 12 | 256 | 8.28e-13 | 68.6 |
| MS.gene05574.t1 | AT3G45440 | 29.070 | 258 | 153 | 10 | 17 | 257 | 12 | 256 | 8.28e-13 | 68.6 |
| MS.gene05574.t1 | AT1G07460 | 28.689 | 244 | 132 | 9 | 41 | 260 | 38 | 263 | 8.99e-13 | 67.8 |
| MS.gene05574.t1 | AT1G70110 | 27.386 | 241 | 140 | 11 | 43 | 262 | 33 | 259 | 9.95e-13 | 68.6 |
| MS.gene05574.t1 | AT3G53380 | 32.685 | 257 | 149 | 12 | 10 | 255 | 1 | 244 | 1.83e-12 | 67.8 |
| MS.gene05574.t1 | AT5G03350 | 28.369 | 282 | 148 | 14 | 14 | 259 | 6 | 269 | 1.94e-12 | 66.2 |
| MS.gene05574.t1 | AT3G45410 | 26.119 | 268 | 168 | 10 | 10 | 260 | 5 | 259 | 9.02e-12 | 65.5 |
| MS.gene05574.t1 | AT4G04960 | 28.440 | 218 | 124 | 6 | 58 | 255 | 48 | 253 | 4.00e-11 | 63.5 |
| MS.gene05574.t1 | AT4G04960 | 28.440 | 218 | 124 | 6 | 58 | 255 | 48 | 253 | 5.69e-11 | 63.2 |
Find 68 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTGTGCTACTTATATTTC+CGG | 0.221234 | 3.2:-54101522 | None:intergenic |
| CTACTTATATTTCCGGGATT+TGG | 0.229840 | 3.2:-54101515 | None:intergenic |
| ATTCTGCCCCTATTCACATT+TGG | 0.267516 | 3.2:+54101258 | MS.gene05574:CDS |
| CTTGGAAGATGAGATTCTTT+TGG | 0.268194 | 3.2:-54101160 | None:intergenic |
| CTTCCCTGTTGAGGTAGTTT+CGG | 0.316382 | 3.2:-54101189 | None:intergenic |
| AATATCATGTGCTGCATATT+CGG | 0.316970 | 3.2:-54101774 | None:intergenic |
| TTCTGCCCCTATTCACATTT+GGG | 0.317850 | 3.2:+54101259 | MS.gene05574:CDS |
| AATCACTGTTCCGTGGAGTT+TGG | 0.318353 | 3.2:+54101583 | MS.gene05574:CDS |
| CCTCCTTGACGAATTGATTT+CGG | 0.320012 | 3.2:-54101401 | None:intergenic |
| TGCTATCCCAAATGTGAATA+GGG | 0.336758 | 3.2:-54101265 | None:intergenic |
| TTGCTATCCCAAATGTGAAT+AGG | 0.349217 | 3.2:-54101266 | None:intergenic |
| AATGTTGTGATAGGGTTTAA+TGG | 0.356600 | 3.2:+54101623 | MS.gene05574:CDS |
| GTGGGTAAGGATTGGTTTCT+CGG | 0.358994 | 3.2:+54101739 | MS.gene05574:CDS |
| GAGTTTGGAAAATAATAAAA+AGG | 0.376135 | 3.2:+54101598 | MS.gene05574:CDS |
| AGTAGCACAGGTCGACATAT+TGG | 0.377327 | 3.2:+54101533 | MS.gene05574:CDS |
| CCTGTGCTACTTATATTTCC+GGG | 0.385267 | 3.2:-54101521 | None:intergenic |
| CACATTTGGGATAGCAAAAC+CGG | 0.385321 | 3.2:+54101272 | MS.gene05574:CDS |
| CACTTTCTATAATGCTCAAT+GGG | 0.387120 | 3.2:+54101490 | MS.gene05574:CDS |
| TTCAAAGTTAGCCACGCTTC+CGG | 0.394516 | 3.2:-54101291 | None:intergenic |
| AAGTCATGTTGTGCCTTTGA+AGG | 0.401051 | 3.2:+54101703 | MS.gene05574:CDS |
| GATGCCGAAACTACCTCAAC+AGG | 0.406566 | 3.2:+54101185 | MS.gene05574:CDS |
| ATTGGTTTCTCGGCTGCGAC+TGG | 0.417093 | 3.2:+54101749 | MS.gene05574:CDS |
| GGATAGGATTAAAGAGAACT+TGG | 0.423638 | 3.2:-54101051 | None:intergenic |
| TACCTTCACCTTTACAATAA+CGG | 0.427007 | 3.2:+54101316 | MS.gene05574:CDS |
| TCAAAGGCACAACATGACTT+AGG | 0.457672 | 3.2:-54101700 | None:intergenic |
| TAGCAAGATGAAGAATGTTA+AGG | 0.459173 | 3.2:-54101078 | None:intergenic |
| AAGGCAGTCAAGAACTCTAT+TGG | 0.460512 | 3.2:+54101224 | MS.gene05574:CDS |
| ACACTTTCTATAATGCTCAA+TGG | 0.461585 | 3.2:+54101489 | MS.gene05574:CDS |
| GTTCCTGAGTGGGTAAGGAT+TGG | 0.467906 | 3.2:+54101731 | MS.gene05574:CDS |
| AAGTTGTTCCTGAGTGGGTA+AGG | 0.471213 | 3.2:+54101726 | MS.gene05574:CDS |
| TCTGCAACATTATAAGTATT+AGG | 0.482296 | 3.2:-54101341 | None:intergenic |
| ATCAACACTCAACACATTTG+TGG | 0.492912 | 3.2:-54101648 | None:intergenic |
| GATGAAGAATGTTAAGGAGA+TGG | 0.494586 | 3.2:-54101072 | None:intergenic |
| TCCATCACAAAGTTTGTCCC+AGG | 0.494662 | 3.2:+54101137 | MS.gene05574:CDS |
| GACAAACTTTGTGATGGAAA+AGG | 0.501076 | 3.2:-54101132 | None:intergenic |
| CTTCATCTTGCTATTGAACA+AGG | 0.506685 | 3.2:+54101088 | MS.gene05574:CDS |
| GCTCAATGGGATCCAAATCC+CGG | 0.507888 | 3.2:+54101503 | MS.gene05574:CDS |
| TTGAACAGAGCTTGAAACAT+TGG | 0.515799 | 3.2:-54101849 | None:intergenic |
| ATTAGGCGCCGTTATTGTAA+AGG | 0.518797 | 3.2:-54101324 | None:intergenic |
| CATTGATGTTATCAAAACCT+AGG | 0.523343 | 3.2:-54101826 | None:intergenic |
| AAAAGGCTAATGTTGTGATA+GGG | 0.526554 | 3.2:+54101615 | MS.gene05574:CDS |
| CAAAGGCACAACATGACTTA+GGG | 0.534314 | 3.2:-54101699 | None:intergenic |
| TAGGATTAAAGAGAACTTGG+AGG | 0.534551 | 3.2:-54101048 | None:intergenic |
| GAAGGAAGTTGTTCCTGAGT+GGG | 0.537596 | 3.2:+54101721 | MS.gene05574:CDS |
| GCCTGGGACAAACTTTGTGA+TGG | 0.542810 | 3.2:-54101138 | None:intergenic |
| AGGTAGTTTCGGCATCGCCT+TGG | 0.542882 | 3.2:-54101178 | None:intergenic |
| TTTGATTCAAAGTTGAACCT+AGG | 0.546087 | 3.2:+54101809 | MS.gene05574:CDS |
| CCCGGAAATATAAGTAGCAC+AGG | 0.548535 | 3.2:+54101521 | MS.gene05574:CDS |
| TCAGGAACAACTTCCTTCAA+AGG | 0.548600 | 3.2:-54101716 | None:intergenic |
| CGCCGTTATTGTAAAGGTGA+AGG | 0.549680 | 3.2:-54101318 | None:intergenic |
| GAGTTCTAACTTCCCTGTTG+AGG | 0.551336 | 3.2:-54101198 | None:intergenic |
| GATTTCGGCTGAGTATCAAT+CGG | 0.563335 | 3.2:-54101386 | None:intergenic |
| AGAATGTTAAGGAGATGGAT+AGG | 0.563697 | 3.2:-54101067 | None:intergenic |
| ATTCGTCAAGGAGGATACCT+TGG | 0.568952 | 3.2:+54101410 | MS.gene05574:CDS |
| CAAAAGAATCTCATCTTCCA+AGG | 0.573627 | 3.2:+54101161 | MS.gene05574:CDS |
| AGGGTATAATGACGAATCAA+AGG | 0.576589 | 3.2:-54101680 | None:intergenic |
| AAACCAATCCTTACCCACTC+AGG | 0.577788 | 3.2:-54101734 | None:intergenic |
| TTCTGCTATTGAAAACTCCA+AGG | 0.589730 | 3.2:-54101427 | None:intergenic |
| TGAAGGAAGTTGTTCCTGAG+TGG | 0.603890 | 3.2:+54101720 | MS.gene05574:CDS |
| AATCAATAATCACTGTTCCG+TGG | 0.606891 | 3.2:+54101576 | MS.gene05574:CDS |
| GCTATCCCAAATGTGAATAG+GGG | 0.608736 | 3.2:-54101264 | None:intergenic |
| AAATGTGTTGAGTGTTGATG+TGG | 0.622877 | 3.2:+54101652 | MS.gene05574:CDS |
| GGATAGCAAAACCGGAAGCG+TGG | 0.638962 | 3.2:+54101280 | MS.gene05574:CDS |
| AGGGAAGTTAGAACTCAGCA+AGG | 0.647782 | 3.2:+54101205 | MS.gene05574:CDS |
| AATACTTATAATGTTGCAGA+CGG | 0.651921 | 3.2:+54101344 | MS.gene05574:CDS |
| CAGCCGAAATCAATTCGTCA+AGG | 0.655408 | 3.2:+54101398 | MS.gene05574:CDS |
| CCGAAATCAATTCGTCAAGG+AGG | 0.688967 | 3.2:+54101401 | MS.gene05574:CDS |
| ATGCCGAAACTACCTCAACA+GGG | 0.739112 | 3.2:+54101186 | MS.gene05574:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAGTTTGGAAAATAATAAAA+AGG | + | chr3.2:54101598-54101617 | MS.gene05574:CDS | 20.0% |
| ! | AATACTTATAATGTTGCAGA+CGG | + | chr3.2:54101344-54101363 | MS.gene05574:CDS | 25.0% |
| ! | TCTGCAACATTATAAGTATT+AGG | - | chr3.2:54101344-54101363 | None:intergenic | 25.0% |
| !! | TTATTATTTTCCAAACTCCA+CGG | - | chr3.2:54101596-54101615 | None:intergenic | 25.0% |
| !!! | CAACAGTTTGAATTGATTTT+TGG | - | chr3.2:54101457-54101476 | None:intergenic | 25.0% |
| AAAAAGGCTAATGTTGTGAT+AGG | + | chr3.2:54101614-54101633 | MS.gene05574:CDS | 30.0% | |
| AAAAGGCTAATGTTGTGATA+GGG | + | chr3.2:54101615-54101634 | MS.gene05574:CDS | 30.0% | |
| AATATCATGTGCTGCATATT+CGG | - | chr3.2:54101777-54101796 | None:intergenic | 30.0% | |
| AATGTTGTGATAGGGTTTAA+TGG | + | chr3.2:54101623-54101642 | MS.gene05574:CDS | 30.0% | |
| ACACTTTCTATAATGCTCAA+TGG | + | chr3.2:54101489-54101508 | MS.gene05574:CDS | 30.0% | |
| CACTTTCTATAATGCTCAAT+GGG | + | chr3.2:54101490-54101509 | MS.gene05574:CDS | 30.0% | |
| CATTGATGTTATCAAAACCT+AGG | - | chr3.2:54101829-54101848 | None:intergenic | 30.0% | |
| TACCTTCACCTTTACAATAA+CGG | + | chr3.2:54101316-54101335 | MS.gene05574:CDS | 30.0% | |
| TAGCAAGATGAAGAATGTTA+AGG | - | chr3.2:54101081-54101100 | None:intergenic | 30.0% | |
| TTTGATTCAAAGTTGAACCT+AGG | + | chr3.2:54101809-54101828 | MS.gene05574:CDS | 30.0% | |
| AATCAATAATCACTGTTCCG+TGG | + | chr3.2:54101576-54101595 | MS.gene05574:CDS | 35.0% | |
| AGAATGTTAAGGAGATGGAT+AGG | - | chr3.2:54101070-54101089 | None:intergenic | 35.0% | |
| AGGGTATAATGACGAATCAA+AGG | - | chr3.2:54101683-54101702 | None:intergenic | 35.0% | |
| ATCAACACTCAACACATTTG+TGG | - | chr3.2:54101651-54101670 | None:intergenic | 35.0% | |
| CAAAAGAATCTCATCTTCCA+AGG | + | chr3.2:54101161-54101180 | MS.gene05574:CDS | 35.0% | |
| CTACTTATATTTCCGGGATT+TGG | - | chr3.2:54101518-54101537 | None:intergenic | 35.0% | |
| GATGAAGAATGTTAAGGAGA+TGG | - | chr3.2:54101075-54101094 | None:intergenic | 35.0% | |
| GGATAGGATTAAAGAGAACT+TGG | - | chr3.2:54101054-54101073 | None:intergenic | 35.0% | |
| TAGGATTAAAGAGAACTTGG+AGG | - | chr3.2:54101051-54101070 | None:intergenic | 35.0% | |
| TGCTATCCCAAATGTGAATA+GGG | - | chr3.2:54101268-54101287 | None:intergenic | 35.0% | |
| TTCTGCTATTGAAAACTCCA+AGG | - | chr3.2:54101430-54101449 | None:intergenic | 35.0% | |
| TTGAACAGAGCTTGAAACAT+TGG | - | chr3.2:54101852-54101871 | None:intergenic | 35.0% | |
| TTGCTATCCCAAATGTGAAT+AGG | - | chr3.2:54101269-54101288 | None:intergenic | 35.0% | |
| ! | CTTGGAAGATGAGATTCTTT+TGG | - | chr3.2:54101163-54101182 | None:intergenic | 35.0% |
| ! | GACAAACTTTGTGATGGAAA+AGG | - | chr3.2:54101135-54101154 | None:intergenic | 35.0% |
| !! | AAATGTGTTGAGTGTTGATG+TGG | + | chr3.2:54101652-54101671 | MS.gene05574:CDS | 35.0% |
| !! | ACCTGTGCTACTTATATTTC+CGG | - | chr3.2:54101525-54101544 | None:intergenic | 35.0% |
| !! | CTTCATCTTGCTATTGAACA+AGG | + | chr3.2:54101088-54101107 | MS.gene05574:CDS | 35.0% |
| !!! | CAGCACATGATATTTTTTCG+TGG | + | chr3.2:54101783-54101802 | MS.gene05574:CDS | 35.0% |
| AAGGCAGTCAAGAACTCTAT+TGG | + | chr3.2:54101224-54101243 | MS.gene05574:CDS | 40.0% | |
| AAGTCATGTTGTGCCTTTGA+AGG | + | chr3.2:54101703-54101722 | MS.gene05574:CDS | 40.0% | |
| ATTCTGCCCCTATTCACATT+TGG | + | chr3.2:54101258-54101277 | MS.gene05574:CDS | 40.0% | |
| GATTTCGGCTGAGTATCAAT+CGG | - | chr3.2:54101389-54101408 | None:intergenic | 40.0% | |
| GCTATCCCAAATGTGAATAG+GGG | - | chr3.2:54101267-54101286 | None:intergenic | 40.0% | |
| TCAGGAACAACTTCCTTCAA+AGG | - | chr3.2:54101719-54101738 | None:intergenic | 40.0% | |
| TTCTGCCCCTATTCACATTT+GGG | + | chr3.2:54101259-54101278 | MS.gene05574:CDS | 40.0% | |
| ! | AAGATGAGATTCTTTTGGCC+TGG | - | chr3.2:54101158-54101177 | None:intergenic | 40.0% |
| ! | AGATGAGATTCTTTTGGCCT+GGG | - | chr3.2:54101157-54101176 | None:intergenic | 40.0% |
| ! | ATTAGGCGCCGTTATTGTAA+AGG | - | chr3.2:54101327-54101346 | None:intergenic | 40.0% |
| ! | CAAAGGCACAACATGACTTA+GGG | - | chr3.2:54101702-54101721 | None:intergenic | 40.0% |
| ! | CACATTTGGGATAGCAAAAC+CGG | + | chr3.2:54101272-54101291 | MS.gene05574:CDS | 40.0% |
| ! | CCTCCTTGACGAATTGATTT+CGG | - | chr3.2:54101404-54101423 | None:intergenic | 40.0% |
| ! | TCAAAGGCACAACATGACTT+AGG | - | chr3.2:54101703-54101722 | None:intergenic | 40.0% |
| !! | CCTGTGCTACTTATATTTCC+GGG | - | chr3.2:54101524-54101543 | None:intergenic | 40.0% |
| AAACCAATCCTTACCCACTC+AGG | - | chr3.2:54101737-54101756 | None:intergenic | 45.0% | |
| AAGTTGTTCCTGAGTGGGTA+AGG | + | chr3.2:54101726-54101745 | MS.gene05574:CDS | 45.0% | |
| AATCACTGTTCCGTGGAGTT+TGG | + | chr3.2:54101583-54101602 | MS.gene05574:CDS | 45.0% | |
| AGGGAAGTTAGAACTCAGCA+AGG | + | chr3.2:54101205-54101224 | MS.gene05574:CDS | 45.0% | |
| AGTAGCACAGGTCGACATAT+TGG | + | chr3.2:54101533-54101552 | MS.gene05574:CDS | 45.0% | |
| ATGCCGAAACTACCTCAACA+GGG | + | chr3.2:54101186-54101205 | MS.gene05574:CDS | 45.0% | |
| ATTCGTCAAGGAGGATACCT+TGG | + | chr3.2:54101410-54101429 | MS.gene05574:CDS | 45.0% | |
| CAGCCGAAATCAATTCGTCA+AGG | + | chr3.2:54101398-54101417 | MS.gene05574:CDS | 45.0% | |
| CCCGGAAATATAAGTAGCAC+AGG | + | chr3.2:54101521-54101540 | MS.gene05574:CDS | 45.0% | |
| CCGAAATCAATTCGTCAAGG+AGG | + | chr3.2:54101401-54101420 | MS.gene05574:CDS | 45.0% | |
| CGCCGTTATTGTAAAGGTGA+AGG | - | chr3.2:54101321-54101340 | None:intergenic | 45.0% | |
| CTTCCCTGTTGAGGTAGTTT+CGG | - | chr3.2:54101192-54101211 | None:intergenic | 45.0% | |
| GAAGGAAGTTGTTCCTGAGT+GGG | + | chr3.2:54101721-54101740 | MS.gene05574:CDS | 45.0% | |
| TCCATCACAAAGTTTGTCCC+AGG | + | chr3.2:54101137-54101156 | MS.gene05574:CDS | 45.0% | |
| TGAAGGAAGTTGTTCCTGAG+TGG | + | chr3.2:54101720-54101739 | MS.gene05574:CDS | 45.0% | |
| TTCAAAGTTAGCCACGCTTC+CGG | - | chr3.2:54101294-54101313 | None:intergenic | 45.0% | |
| ! | GAGTTCTAACTTCCCTGTTG+AGG | - | chr3.2:54101201-54101220 | None:intergenic | 45.0% |
| !! | GTGGGTAAGGATTGGTTTCT+CGG | + | chr3.2:54101739-54101758 | MS.gene05574:CDS | 45.0% |
| GATGCCGAAACTACCTCAAC+AGG | + | chr3.2:54101185-54101204 | MS.gene05574:CDS | 50.0% | |
| GCCTGGGACAAACTTTGTGA+TGG | - | chr3.2:54101141-54101160 | None:intergenic | 50.0% | |
| GCTCAATGGGATCCAAATCC+CGG | + | chr3.2:54101503-54101522 | MS.gene05574:CDS | 50.0% | |
| GTTCCTGAGTGGGTAAGGAT+TGG | + | chr3.2:54101731-54101750 | MS.gene05574:CDS | 50.0% | |
| AGGTAGTTTCGGCATCGCCT+TGG | - | chr3.2:54101181-54101200 | None:intergenic | 55.0% | |
| GGATAGCAAAACCGGAAGCG+TGG | + | chr3.2:54101280-54101299 | MS.gene05574:CDS | 55.0% | |
| ! | ATTGGTTTCTCGGCTGCGAC+TGG | + | chr3.2:54101749-54101768 | MS.gene05574:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 54101041 | 54101880 | 54101041 | ID=MS.gene05574 |
| chr3.2 | mRNA | 54101041 | 54101880 | 54101041 | ID=MS.gene05574.t1;Parent=MS.gene05574 |
| chr3.2 | exon | 54101041 | 54101880 | 54101041 | ID=MS.gene05574.t1.exon1;Parent=MS.gene05574.t1 |
| chr3.2 | CDS | 54101041 | 54101880 | 54101041 | ID=cds.MS.gene05574.t1;Parent=MS.gene05574.t1 |
| Gene Sequence |
| Protein sequence |
