AlfalfaGEDB | MS.gene41211

M. sativa cultivar XinJiangDaYe / MS.gene41211

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564
PFAM: PF04564.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41211.t1	XP_013467848.1	96.9	228	7	0	1	228	19	246	5.60E-120	440.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41211.t1	Q9SF15	40.9	232	123	5	1	224	23	248	1.2e-43	177.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41211.t1	A0A072VUZ2	96.9	228	7	0	1	228	19	246	4.0e-120	440.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene034753	MS.gene41211	PPI
MS.gene41032	MS.gene41211	PPI
MS.gene45454	MS.gene41211	PPI
MS.gene41033	MS.gene41211	PPI
MS.gene025667	MS.gene41211	PPI
MS.gene007121	MS.gene41211	PPI
MS.gene066130	MS.gene41211	PPI
MS.gene45456	MS.gene41211	PPI
MS.gene45455	MS.gene41211	PPI
MS.gene034751	MS.gene41211	PPI
MS.gene26489	MS.gene41211	PPI
MS.gene066129	MS.gene41211	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41211.t1	MTR_1g056910	96.930	228	7	0	1	228	19	246	1.16e-162	455
MS.gene41211.t1	MTR_1g056880	39.216	255	121	6	1	224	20	271	1.23e-49	167
MS.gene41211.t1	MTR_7g106340	42.194	237	121	7	1	224	21	254	2.88e-49	166
MS.gene41211.t1	MTR_1g056870	38.431	255	123	6	1	224	20	271	1.85e-48	164
MS.gene41211.t1	MTR_1g056840	38.039	255	124	6	1	224	20	271	1.93e-48	164
MS.gene41211.t1	MTR_2g087350	44.444	234	115	8	1	228	19	243	8.03e-45	154
MS.gene41211.t1	MTR_4g051515	40.529	227	128	5	1	221	19	244	7.55e-42	147
MS.gene41211.t1	MTR_5g077510	37.073	205	115	6	1	196	48	247	2.39e-35	130
MS.gene41211.t1	MTR_3g065080	45.238	126	61	4	1	120	49	172	4.12e-29	113
MS.gene41211.t1	MTR_6g071340	44.000	125	62	3	1	119	18	140	6.58e-28	109
MS.gene41211.t1	MTR_5g048050	43.200	125	63	3	1	119	38	160	2.52e-27	108
MS.gene41211.t1	MTR_4g085720	56.818	88	36	2	1	88	27	112	2.34e-24	100
MS.gene41211.t1	MTR_3g095730	31.633	196	113	9	1	182	26	214	2.28e-15	74.7
MS.gene41211.t1	MTR_8g092870	33.493	209	114	10	1	194	23	221	3.92e-15	73.9
MS.gene41211.t1	MTR_4g063800	37.405	131	66	5	1	119	268	394	1.20e-13	70.1
MS.gene41211.t1	MTR_3g096370	39.167	120	55	4	1	112	23	132	3.01e-13	68.6
MS.gene41211.t1	MTR_7g078330	35.484	124	71	5	1	118	287	407	6.33e-13	67.8
MS.gene41211.t1	MTR_7g059405	34.783	138	78	5	1	128	277	412	7.39e-12	64.7
MS.gene41211.t1	MTR_1g017770	40.164	122	60	6	1	118	58	170	8.10e-12	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene41211.t1	AT3G11840	40.948	232	123	5	1	224	23	248	1.99e-54	181
MS.gene41211.t1	AT2G35930	41.228	228	122	6	1	224	25	244	2.05e-46	159
MS.gene41211.t1	AT3G52450	51.852	135	60	3	1	131	20	153	1.11e-38	139
MS.gene41211.t1	AT3G19380	38.172	186	90	6	1	165	27	208	2.70e-29	113
MS.gene41211.t1	AT1G49780	45.926	135	63	5	1	128	27	158	6.39e-26	104
MS.gene41211.t1	AT1G66160	41.600	125	65	3	1	119	46	168	1.31e-24	100
MS.gene41211.t1	AT5G37490	37.017	181	82	7	1	175	44	198	1.10e-22	95.5
MS.gene41211.t1	AT3G18710	32.558	215	127	8	1	207	25	229	1.44e-16	78.2
MS.gene41211.t1	AT1G66160	36.893	103	58	2	23	119	51	152	1.10e-14	72.8
MS.gene41211.t1	AT5G64660	36.066	122	62	5	1	114	23	136	2.98e-12	65.5

Find 24 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACAATTCTTTCGAAGAATT+CGG	0.199521	1.2:+45035279	None:intergenic
TGATTCCTTTGAGAAGCTTT+TGG	0.238517	1.2:+45035668	None:intergenic
ATTTGAAAATAATCAAATAC+TGG	0.314035	1.2:-45035425	MS.gene41211:CDS
ATTCCTGTTATAGCAGTAAC+AGG	0.346346	1.2:+45035898	None:intergenic
GATCCTGTTACTGCTATAAC+AGG	0.386049	1.2:-45035901	MS.gene41211:CDS
GGTGTTAAATCAGAATCTAA+TGG	0.418840	1.2:+45035796	None:intergenic
GCTCAAGAGTTTAATGCAGT+TGG	0.418910	1.2:-45035629	MS.gene41211:CDS
ATTGTGAAAGTTCTAAGAAA+TGG	0.433892	1.2:-45035262	MS.gene41211:CDS
ACAATTCTTTCGAAGAATTC+GGG	0.437523	1.2:+45035280	None:intergenic
AAACTCTTGAGCTGAACCTT+AGG	0.446427	1.2:+45035640	None:intergenic
ATGAACATAATCATCTCTTT+CGG	0.450958	1.2:+45035550	None:intergenic
TTCGTCGTTTGATTCAATCT+TGG	0.454949	1.2:-45035762	MS.gene41211:CDS
GAAGGTATTGAAGAAGCTCT+TGG	0.482882	1.2:-45035496	MS.gene41211:CDS
CAAAGGAATCAAAGATCCTA+AGG	0.507179	1.2:-45035656	MS.gene41211:CDS
AGATTCTTCACTTCTTCTGT+AGG	0.513088	1.2:+45035451	None:intergenic
AAACGACGAAGTGTGTGATT+TGG	0.523610	1.2:+45035775	None:intergenic
GGAAGAGGTTGTTGTGTGAC+AGG	0.532069	1.2:+45035817	None:intergenic
TGCACACAAAATGCTTCACT+TGG	0.539364	1.2:-45035739	MS.gene41211:CDS
AAATCAGAATCTAATGGAAG+AGG	0.553606	1.2:+45035802	None:intergenic
AAATACTGGATTCTTTAACA+TGG	0.565062	1.2:-45035411	MS.gene41211:CDS
ATCGAGACAGCATTGAACAT+TGG	0.589905	1.2:-45035867	MS.gene41211:CDS
CAAGTCCAAAAGCTTCTCAA+AGG	0.606638	1.2:-45035673	MS.gene41211:CDS
GGTTACTGTGAAATCGCATG+CGG	0.706124	1.2:-45035362	MS.gene41211:CDS
AATGGTGTAATAACACAACA+AGG	0.719258	1.2:-45035244	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTGAAAATAATCAAATAC+TGG	-	chr1.2:45035727-45035746	MS.gene41211:CDS	15.0%
!!	ATAGTTGATTGTTACAAAAA+AGG	-	chr1.2:45035623-45035642	MS.gene41211:CDS	20.0%
!!!	TGGTTTTTTACAATTTGTTA+AGG	-	chr1.2:45035676-45035695	MS.gene41211:CDS	20.0%
!	AAAAAAGGCGAAATTTATGA+AGG	-	chr1.2:45035638-45035657	MS.gene41211:CDS	25.0%
!	AAATACTGGATTCTTTAACA+TGG	-	chr1.2:45035741-45035760	MS.gene41211:CDS	25.0%
!	ATGAACATAATCATCTCTTT+CGG	+	chr1.2:45035605-45035624	None:intergenic	25.0%
!!	AGAAAGATTTTGTTGGAAAA+TGG	-	chr1.2:45035575-45035594	MS.gene41211:CDS	25.0%
!!!	ATTGTGAAAGTTCTAAGAAA+TGG	-	chr1.2:45035890-45035909	MS.gene41211:CDS	25.0%
	AAATCAGAATCTAATGGAAG+AGG	+	chr1.2:45035353-45035372	None:intergenic	30.0%
	ACAATTCTTTCGAAGAATTC+GGG	+	chr1.2:45035875-45035894	None:intergenic	30.0%
	CACAATTCTTTCGAAGAATT+CGG	+	chr1.2:45035876-45035895	None:intergenic	30.0%
	GGTGTTAAATCAGAATCTAA+TGG	+	chr1.2:45035359-45035378	None:intergenic	30.0%
!	GAGCAATAGAAAGATTTTGT+TGG	-	chr1.2:45035568-45035587	MS.gene41211:CDS	30.0%
	AGATTCTTCACTTCTTCTGT+AGG	+	chr1.2:45035704-45035723	None:intergenic	35.0%
	AGTTTCCGAGATGAAAAACT+CGG	-	chr1.2:45035769-45035788	MS.gene41211:CDS	35.0%
	ATTCCTGTTATAGCAGTAAC+AGG	+	chr1.2:45035257-45035276	None:intergenic	35.0%
	CAAAGGAATCAAAGATCCTA+AGG	-	chr1.2:45035496-45035515	MS.gene41211:CDS	35.0%
	TTCGTCGTTTGATTCAATCT+TGG	-	chr1.2:45035390-45035409	MS.gene41211:CDS	35.0%
!	ACTTGAATTTTTGTGAGTGG+TGG	+	chr1.2:45035464-45035483	None:intergenic	35.0%
!	AGTAACCGAGTTTTTCATCT+CGG	+	chr1.2:45035777-45035796	None:intergenic	35.0%
!	ATTTTTGTGAGTGGTGGTTT+TGG	+	chr1.2:45035458-45035477	None:intergenic	35.0%
!	TGGACTTGAATTTTTGTGAG+TGG	+	chr1.2:45035467-45035486	None:intergenic	35.0%
!!	TGATTCCTTTGAGAAGCTTT+TGG	+	chr1.2:45035487-45035506	None:intergenic	35.0%
	AAACGACGAAGTGTGTGATT+TGG	+	chr1.2:45035380-45035399	None:intergenic	40.0%
	AAACTCTTGAGCTGAACCTT+AGG	+	chr1.2:45035515-45035534	None:intergenic	40.0%
	CAAGTCCAAAAGCTTCTCAA+AGG	-	chr1.2:45035479-45035498	MS.gene41211:CDS	40.0%
	GATCCTGTTACTGCTATAAC+AGG	-	chr1.2:45035251-45035270	MS.gene41211:CDS	40.0%
	GCTCAAGAGTTTAATGCAGT+TGG	-	chr1.2:45035523-45035542	MS.gene41211:CDS	40.0%
	TGCACACAAAATGCTTCACT+TGG	-	chr1.2:45035413-45035432	MS.gene41211:CDS	40.0%
!	ATCGAGACAGCATTGAACAT+TGG	-	chr1.2:45035285-45035304	MS.gene41211:CDS	40.0%
!!	GAAGGTATTGAAGAAGCTCT+TGG	-	chr1.2:45035656-45035675	MS.gene41211:CDS	40.0%
!!!	GCGATTCAATCGTTTTAGAG+AGG	-	chr1.2:45035843-45035862	MS.gene41211:CDS	40.0%
	GGTTACTGTGAAATCGCATG+CGG	-	chr1.2:45035790-45035809	MS.gene41211:CDS	45.0%
!!!	TGAGTGGTGGTTTTGGTGTT+GGG	+	chr1.2:45035451-45035470	None:intergenic	45.0%
	GGAAGAGGTTGTTGTGTGAC+AGG	+	chr1.2:45035338-45035357	None:intergenic	50.0%
!!!	GTGAGTGGTGGTTTTGGTGT+TGG	+	chr1.2:45035452-45035471	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	45035245	45035929	45035245	ID=MS.gene41211
chr1.2	mRNA	45035245	45035929	45035245	ID=MS.gene41211.t1;Parent=MS.gene41211
chr1.2	exon	45035245	45035929	45035245	ID=MS.gene41211.t1.exon1;Parent=MS.gene41211.t1
chr1.2	CDS	45035245	45035929	45035245	ID=cds.MS.gene41211.t1;Parent=MS.gene41211.t1

Gene Sequence

>MS.gene41211

ATGAAAGATCCTGTTACTGCTATAACAGGAATAACATATGATCGAGACAGCATTGAACATTGGTTATTCACAAACAAGAACACAACATGTCCTGTCACACAACAACCTCTTCCATTAGATTCTGATTTAACACCAAATCACACACTTCGTCGTTTGATTCAATCTTGGTGCACACAAAATGCTTCACTTGGTATTGATAGAATCCCAACACCAAAACCACCACTCACAAAAATTCAAGTCCAAAAGCTTCTCAAAGGAATCAAAGATCCTAAGGTTCAGCTCAAGAGTTTAATGCAGTTGGAATTGCTTGCTGCTGAGAAAGAGAGCAATAGAAAGATTTTGTTGGAAAATGGTGTGCCGAAAGAGATGATTATGTTCATAGTTGATTGTTACAAAAAAGGCGAAATTTATGAAGGTATTGAAGAAGCTCTTGGTTTTTTACAATTTGTTAAGGTTCCTACAGAAGAAGTGAAGAATCTTCTATTTGAAAATAATCAAATACTGGATTCTTTAACATGGATTTTAGTTTCCGAGATGAAAAACTCGGTTACTGTGAAATCGCATGCGGTTTTAGTGCTTAAAAAATTCATAAACAAATGCGATTCAATCGTTTTAGAGAGGCTAAAACCCGAATTCTTCGAAAGAATTGTGAAAGTTCTAAGAAATGGTGTAATAACACAACAAG

Protein sequence

>MS.gene41211.t1

MKDPVTAITGITYDRDSIEHWLFTNKNTTCPVTQQPLPLDSDLTPNHTLRRLIQSWCTQNASLGIDRIPTPKPPLTKIQVQKLLKGIKDPKVQLKSLMQLELLAAEKESNRKILLENGVPKEMIMFIVDCYKKGEIYEGIEEALGFLQFVKVPTEEVKNLLFENNQILDSLTWILVSEMKNSVTVKSHAVLVLKKFINKCDSIVLERLKPEFFERIVKVLRNGVITQQ