AlfalfaGEDB | MS.gene066130

M. sativa cultivar XinJiangDaYe / MS.gene066130

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564
PFAM: PF04564.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066130.t1	RHN79430.1	96.1	441	13	2	1	441	1	437	6.10E-232	813.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066130.t1	Q9SVC6	54.3	446	184	7	1	438	1	434	1.5e-122	441.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066130.t1	A0A396JMB5	96.1	441	13	2	1	441	1	437	4.4e-232	813.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049281	MS.gene066130	0.800669	1.29E-48	-1.69E-46
MS.gene050095	MS.gene066130	0.811816	5.79E-51	-1.69E-46
MS.gene050381	MS.gene066130	0.840367	9.07E-58	-1.69E-46
MS.gene050612	MS.gene066130	-0.806566	7.73E-50	-1.69E-46
MS.gene050868	MS.gene066130	-0.818608	1.79E-52	-1.69E-46
MS.gene050871	MS.gene066130	-0.816582	5.13E-52	-1.69E-46
MS.gene050883	MS.gene066130	0.806161	9.41E-50	-1.69E-46
MS.gene051044	MS.gene066130	0.826541	2.56E-54	-1.69E-46
MS.gene051127	MS.gene066130	0.851128	1.09E-60	-1.69E-46
MS.gene051138	MS.gene066130	0.842972	1.87E-58	-1.69E-46
MS.gene051946	MS.gene066130	0.831809	1.35E-55	-1.69E-46
MS.gene052083	MS.gene066130	0.807696	4.46E-50	-1.69E-46
MS.gene052211	MS.gene066130	0.819608	1.06E-52	-1.69E-46
MS.gene053420	MS.gene066130	0.824728	6.90E-54	-1.69E-46
MS.gene053488	MS.gene066130	0.831206	1.90E-55	-1.69E-46
MS.gene053561	MS.gene066130	0.831942	1.25E-55	-1.69E-46
MS.gene053887	MS.gene066130	0.833477	5.21E-56	-1.69E-46
MS.gene054054	MS.gene066130	0.807719	4.41E-50	-1.69E-46
MS.gene054708	MS.gene066130	0.807796	4.24E-50	-1.69E-46
MS.gene05470	MS.gene066130	-0.801012	1.10E-48	-1.69E-46
MS.gene055124	MS.gene066130	0.840693	7.46E-58	-1.69E-46
MS.gene055139	MS.gene066130	0.821626	3.65E-53	-1.69E-46
MS.gene055154	MS.gene066130	0.838965	2.10E-57	-1.69E-46
MS.gene055202	MS.gene066130	0.82078	5.72E-53	-1.69E-46
MS.gene055502	MS.gene066130	-0.803346	3.64E-49	-1.69E-46
MS.gene05589	MS.gene066130	-0.827656	1.39E-54	-1.69E-46
MS.gene055977	MS.gene066130	0.8058	1.12E-49	-1.69E-46
MS.gene056224	MS.gene066130	0.815448	9.20E-52	-1.69E-46
MS.gene056310	MS.gene066130	0.808523	2.97E-50	-1.69E-46
MS.gene056311	MS.gene066130	0.810293	1.24E-50	-1.69E-46
MS.gene056313	MS.gene066130	0.805885	1.08E-49	-1.69E-46
MS.gene056504	MS.gene066130	-0.816501	5.35E-52	-1.69E-46
MS.gene056856	MS.gene066130	0.804216	2.40E-49	-1.69E-46
MS.gene057014	MS.gene066130	0.818272	2.14E-52	-1.69E-46
MS.gene057293	MS.gene066130	0.808756	2.65E-50	-1.69E-46
MS.gene058099	MS.gene066130	0.822768	1.98E-53	-1.69E-46
MS.gene058109	MS.gene066130	0.801114	1.05E-48	-1.69E-46
MS.gene058171	MS.gene066130	0.820001	8.64E-53	-1.69E-46
MS.gene058173	MS.gene066130	0.817228	3.68E-52	-1.69E-46
MS.gene058175	MS.gene066130	0.822622	2.15E-53	-1.69E-46
MS.gene058603	MS.gene066130	0.842606	2.33E-58	-1.69E-46
MS.gene059045	MS.gene066130	0.812781	3.57E-51	-1.69E-46
MS.gene059223	MS.gene066130	0.809832	1.56E-50	-1.69E-46
MS.gene059690	MS.gene066130	0.837999	3.73E-57	-1.69E-46
MS.gene059691	MS.gene066130	0.840005	1.13E-57	-1.69E-46
MS.gene059694	MS.gene066130	0.84864	5.39E-60	-1.69E-46
MS.gene059697	MS.gene066130	0.851606	7.95E-61	-1.69E-46
MS.gene059865	MS.gene066130	-0.808255	3.39E-50	-1.69E-46
MS.gene059916	MS.gene066130	0.823506	1.34E-53	-1.69E-46
MS.gene06077	MS.gene066130	-0.819077	1.40E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene066125	MS.gene066130	PPI
MS.gene93972	MS.gene066130	PPI
MS.gene022190	MS.gene066130	PPI
MS.gene061546	MS.gene066130	PPI
MS.gene017373	MS.gene066130	PPI
MS.gene042912	MS.gene066130	PPI
MS.gene048677	MS.gene066130	PPI
MS.gene41035	MS.gene066130	PPI
MS.gene008306	MS.gene066130	PPI
MS.gene066130	MS.gene41211	PPI
MS.gene04998	MS.gene066130	PPI
MS.gene066130	MS.gene071765	PPI
MS.gene26139	MS.gene066130	PPI
MS.gene066130	MS.gene72611	PPI
MS.gene061543	MS.gene066130	PPI
MS.gene034756	MS.gene066130	PPI
MS.gene41036	MS.gene066130	PPI
MS.gene064492	MS.gene066130	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066130.t1	MTR_1g056880	95.918	441	14	2	1	441	1	437	0.0	858
MS.gene066130.t1	MTR_1g056870	93.878	441	23	2	1	441	1	437	0.0	819
MS.gene066130.t1	MTR_1g056840	89.342	441	17	3	1	441	1	411	0.0	771
MS.gene066130.t1	MTR_7g106340	64.773	440	131	7	1	438	1	418	0.0	543
MS.gene066130.t1	MTR_2g087350	54.023	435	166	4	4	438	3	403	6.46e-151	434
MS.gene066130.t1	MTR_1g056910	40.278	432	218	8	4	430	3	399	1.20e-101	309
MS.gene066130.t1	MTR_4g051515	37.188	441	238	8	4	437	3	411	1.31e-91	283
MS.gene066130.t1	MTR_3g065080	33.113	453	237	12	3	437	32	436	2.64e-58	197
MS.gene066130.t1	MTR_5g077510	32.955	440	238	15	4	429	32	428	4.13e-53	184
MS.gene066130.t1	MTR_5g048050	31.860	430	246	12	2	418	20	415	1.78e-51	179
MS.gene066130.t1	MTR_6g071340	31.190	420	244	11	11	418	9	395	1.72e-50	176
MS.gene066130.t1	MTR_4g085720	30.841	428	249	11	1	423	8	393	4.30e-44	159
MS.gene066130.t1	MTR_8g092870	28.507	442	270	12	5	431	8	418	2.94e-33	130
MS.gene066130.t1	MTR_3g095730	28.217	443	268	12	5	431	11	419	8.56e-30	120
MS.gene066130.t1	MTR_4g063800	25.408	429	262	13	11	430	259	638	6.70e-27	114
MS.gene066130.t1	MTR_7g059405	27.206	408	253	13	11	418	268	631	4.13e-26	111
MS.gene066130.t1	MTR_4g485520	27.900	319	168	9	11	315	109	379	2.85e-25	108
MS.gene066130.t1	MTR_3g096370	27.674	430	262	16	4	423	7	397	3.05e-25	107
MS.gene066130.t1	MTR_4g485520	27.900	319	168	9	11	315	277	547	5.03e-25	108
MS.gene066130.t1	MTR_5g015210	25.891	421	264	9	6	426	66	438	4.60e-24	104
MS.gene066130.t1	MTR_2g018010	28.483	323	164	11	11	313	296	571	7.84e-24	105
MS.gene066130.t1	MTR_7g078330	28.975	283	164	9	11	274	278	542	1.61e-22	100
MS.gene066130.t1	MTR_4g107010	28.029	421	248	18	11	418	284	662	7.15e-22	99.0
MS.gene066130.t1	MTR_5g083030	25.424	354	199	10	11	342	297	607	2.02e-21	97.4
MS.gene066130.t1	MTR_1g094215	28.739	341	182	13	11	339	68	359	6.10e-20	92.0
MS.gene066130.t1	MTR_3g085610	25.427	468	284	18	13	441	286	727	8.53e-20	92.8
MS.gene066130.t1	MTR_1g094215	28.739	341	182	13	11	339	251	542	1.88e-19	91.3
MS.gene066130.t1	MTR_5g034440	27.642	369	232	13	11	375	284	621	1.81e-18	88.6
MS.gene066130.t1	MTR_3g078160	25.240	416	264	13	11	418	279	655	3.22e-18	87.8
MS.gene066130.t1	MTR_3g078160	25.240	416	264	13	11	418	279	655	3.43e-18	87.8
MS.gene066130.t1	MTR_4g091880	26.341	410	247	13	11	418	9	365	1.60e-17	84.3
MS.gene066130.t1	MTR_8g080280	27.069	447	258	22	13	418	288	707	2.97e-17	84.7
MS.gene066130.t1	MTR_4g028960	35.673	171	76	8	2	151	273	430	4.45e-17	84.3
MS.gene066130.t1	MTR_7g097020	45.679	81	37	2	5	85	239	312	6.85e-16	80.5
MS.gene066130.t1	MTR_7g053260	42.500	80	39	2	1	80	72	144	8.57e-16	79.7
MS.gene066130.t1	MTR_7g116600	45.745	94	40	4	11	104	82	164	1.09e-15	79.3
MS.gene066130.t1	MTR_1g069845	45.455	77	35	2	5	81	122	191	2.49e-15	78.6
MS.gene066130.t1	MTR_3g115670	26.047	215	117	7	11	207	34	224	3.83e-15	78.2
MS.gene066130.t1	MTR_1g069845	45.455	77	35	2	5	81	243	312	3.97e-15	78.2
MS.gene066130.t1	MTR_1g069845	45.455	77	35	2	5	81	239	308	4.40e-15	78.2
MS.gene066130.t1	MTR_4g091880	24.390	410	227	13	11	418	9	337	4.44e-15	76.6
MS.gene066130.t1	MTR_1g017770	50.667	75	30	2	6	80	44	111	6.08e-15	77.0
MS.gene066130.t1	MTR_8g077205	44.086	93	39	4	5	95	159	240	1.13e-14	76.6
MS.gene066130.t1	MTR_8g077205	44.086	93	39	4	5	95	271	352	1.55e-14	76.3
MS.gene066130.t1	MTR_2g011140	25.472	318	193	8	13	330	21	294	3.92e-14	73.9
MS.gene066130.t1	MTR_3g078160	46.154	91	41	3	11	100	279	362	4.06e-14	74.7
MS.gene066130.t1	MTR_5g020570	43.878	98	40	6	1	95	265	350	1.14e-13	73.6
MS.gene066130.t1	MTR_5g020570	43.878	98	40	6	1	95	280	365	1.45e-13	73.2
MS.gene066130.t1	MTR_8g103227	39.326	89	40	3	11	99	399	473	6.23e-13	71.6
MS.gene066130.t1	MTR_3g466220	41.667	84	42	2	2	85	228	304	9.29e-13	70.9
MS.gene066130.t1	MTR_3g466220	41.667	84	42	2	2	85	207	283	9.60e-13	70.9
MS.gene066130.t1	MTR_1g090320	43.243	74	36	2	11	84	517	584	1.64e-12	70.5
MS.gene066130.t1	MTR_1g090320	41.250	80	41	2	11	90	519	592	1.66e-12	70.5
MS.gene066130.t1	MTR_1g079450	38.462	91	45	4	5	95	61	140	1.84e-12	69.3
MS.gene066130.t1	MTR_7g117890	41.667	84	42	2	2	85	250	326	8.19e-12	67.8
MS.gene066130.t1	MTR_1g093995	46.479	71	28	3	20	90	1	61	6.95e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066130.t1	AT3G52450	54.054	444	188	6	1	438	1	434	3.08e-157	452
MS.gene066130.t1	AT2G35930	51.584	442	173	5	1	438	6	410	1.99e-154	444
MS.gene066130.t1	AT3G11840	34.968	469	239	9	3	435	6	444	2.23e-77	248
MS.gene066130.t1	AT3G19380	33.487	433	238	10	1	423	8	400	8.55e-50	175
MS.gene066130.t1	AT5G37490	30.275	436	253	14	4	429	28	422	8.95e-47	167
MS.gene066130.t1	AT1G49780	32.589	448	245	12	1	432	8	414	1.95e-44	160
MS.gene066130.t1	AT1G66160	29.384	422	249	13	4	421	30	406	5.45e-42	154
MS.gene066130.t1	AT1G66160	26.777	422	244	14	4	421	30	390	1.69e-29	119
MS.gene066130.t1	AT5G09800	29.213	445	254	16	5	431	8	409	5.37e-29	118
MS.gene066130.t1	AT1G23030	26.555	418	249	14	11	422	245	610	2.28e-28	118
MS.gene066130.t1	AT2G28830	28.369	423	262	18	2	421	178	562	3.36e-28	117
MS.gene066130.t1	AT3G18710	27.314	443	269	12	5	431	10	415	2.06e-26	110
MS.gene066130.t1	AT5G64660	26.906	446	274	13	5	431	8	420	1.70e-24	105
MS.gene066130.t1	AT1G71020	26.887	424	249	15	11	422	99	473	2.54e-24	105
MS.gene066130.t1	AT1G71020	26.995	426	246	14	11	422	247	621	4.54e-24	105
MS.gene066130.t1	AT5G42340	29.054	296	167	8	11	306	294	546	4.78e-24	105
MS.gene066130.t1	AT3G46510	26.950	423	263	17	2	421	250	629	1.50e-23	103
MS.gene066130.t1	AT3G54850	29.739	306	180	12	11	316	252	522	1.70e-23	103
MS.gene066130.t1	AT3G49810	24.292	424	273	9	6	429	63	438	1.04e-21	97.4
MS.gene066130.t1	AT5G65920	24.648	426	269	10	6	429	59	434	1.42e-21	97.1
MS.gene066130.t1	AT5G65920	24.648	426	269	10	6	429	59	434	1.42e-21	97.1
MS.gene066130.t1	AT1G67530	26.770	452	260	14	13	418	278	704	7.11e-21	95.9
MS.gene066130.t1	AT1G67530	26.770	452	260	14	13	418	278	704	7.11e-21	95.9
MS.gene066130.t1	AT1G67530	26.770	452	260	14	13	418	278	704	7.11e-21	95.9
MS.gene066130.t1	AT1G29340	27.245	323	179	10	5	313	303	583	1.26e-19	92.0
MS.gene066130.t1	AT5G01830	26.518	313	178	10	4	308	271	539	8.30e-19	89.7
MS.gene066130.t1	AT1G24330	25.607	453	260	20	13	418	281	703	1.55e-17	85.9
MS.gene066130.t1	AT1G24330	25.607	453	260	20	13	418	281	703	1.55e-17	85.9
MS.gene066130.t1	AT1G67530	48.352	91	35	4	13	103	278	356	2.03e-17	85.1
MS.gene066130.t1	AT1G27910	43.810	105	48	4	13	117	285	378	3.82e-17	84.7
MS.gene066130.t1	AT1G10560	25.079	315	183	9	11	313	292	565	4.77e-17	84.3
MS.gene066130.t1	AT4G21350	25.000	404	249	12	11	412	9	360	1.52e-15	78.2
MS.gene066130.t1	AT3G07360	48.000	75	32	2	11	85	78	145	2.04e-14	75.5
MS.gene066130.t1	AT3G54790	43.750	80	38	2	5	84	200	272	6.52e-14	74.3
MS.gene066130.t1	AT3G54790	43.750	80	38	2	5	84	236	308	8.14e-14	74.3
MS.gene066130.t1	AT3G54790	43.750	80	38	2	5	84	236	308	8.14e-14	74.3
MS.gene066130.t1	AT1G60190	24.513	359	208	14	13	354	284	596	1.06e-13	73.6
MS.gene066130.t1	AT2G23140	40.000	90	43	3	11	100	237	315	3.49e-13	72.4
MS.gene066130.t1	AT2G23140	40.000	90	43	3	11	100	234	312	3.61e-13	72.0
MS.gene066130.t1	AT2G23140	40.000	90	43	3	11	100	234	312	3.61e-13	72.0
MS.gene066130.t1	AT2G23140	40.000	90	43	3	11	100	45	123	4.14e-13	71.6
MS.gene066130.t1	AT2G23140	40.000	90	43	3	11	100	45	123	4.14e-13	71.6
MS.gene066130.t1	AT1G01660	40.000	75	38	2	11	85	502	569	5.65e-12	68.2
MS.gene066130.t1	AT1G01660	40.000	75	38	2	11	85	501	568	5.74e-12	68.2
MS.gene066130.t1	AT3G02840	23.496	349	222	10	83	417	34	351	5.96e-12	67.4
MS.gene066130.t1	AT1G01680	40.260	77	39	2	11	87	237	306	1.65e-11	65.5
MS.gene066130.t1	AT2G45920	40.244	82	42	2	6	87	324	398	3.15e-11	65.5
MS.gene066130.t1	AT5G67340	32.331	133	66	4	5	137	238	346	6.10e-11	65.1
MS.gene066130.t1	AT5G67340	32.331	133	66	4	5	137	238	346	6.10e-11	65.1
MS.gene066130.t1	AT5G18340	36.364	110	57	5	5	114	72	168	6.28e-11	64.7

Find 48 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGATGCTTTCTTTGATATC+TGG	0.244295	1.4:-47183184	None:intergenic
TTCATGAGTTGTAGTGAAAT+AGG	0.280796	1.4:-47182405	None:intergenic
TATGAAGCAAGTAAATTAAA+AGG	0.308972	1.4:-47183653	None:intergenic
TGCACCATGAATTCTTCTTA+TGG	0.309841	1.4:+47182606	MS.gene066130:CDS
TTCAATGAAATTATTGCAAA+AGG	0.321472	1.4:+47183037	MS.gene066130:CDS
TTAATTCAACTTGTCGAATT+CGG	0.334269	1.4:+47183224	MS.gene066130:CDS
GAAGCCATGTGAGATGATTT+TGG	0.361012	1.4:+47183322	MS.gene066130:CDS
GTGGTGTAACTTCGATATCT+TGG	0.376999	1.4:-47182371	None:intergenic
AACTCCATAAGAAGAATTCA+TGG	0.378719	1.4:-47182610	None:intergenic
TTAAATTGCATTCAAAGTCT+TGG	0.383883	1.4:+47183612	MS.gene066130:CDS
ATGCCTGTTGAGACAGTAAC+TGG	0.387709	1.4:-47182432	None:intergenic
AGAGTAAAAGCAATTGAATC+AGG	0.420580	1.4:+47183254	MS.gene066130:CDS
TCATGAGTTGTAGTGAAATA+GGG	0.428583	1.4:-47182404	None:intergenic
TGTTGTTGCTAAGCTTTGTT+TGG	0.429305	1.4:+47183538	MS.gene066130:CDS
GTTCAGGAAATGTTGAGAAT+TGG	0.434841	1.4:+47183515	MS.gene066130:CDS
TTCTTCTTGATTGCAAAGAA+AGG	0.442698	1.4:+47183300	MS.gene066130:CDS
TTTGATGATGATGATGTTGA+AGG	0.466030	1.4:+47182891	MS.gene066130:CDS
GATCCAGTTACTGTCTCAAC+AGG	0.466859	1.4:+47182429	MS.gene066130:CDS
AATTCATGGTGCACCATGCT+TGG	0.475436	1.4:-47182596	None:intergenic
TCAATGAAATTATTGCAAAA+GGG	0.499783	1.4:+47183038	MS.gene066130:CDS
TTGGTTCTTCAAGTTGATAG+TGG	0.510674	1.4:+47183557	MS.gene066130:CDS
AGTCTACGAAGAGTGTGGTT+TGG	0.516469	1.4:-47182570	None:intergenic
TTTGCTGAAATCTATATCAA+AGG	0.516943	1.4:+47183091	MS.gene066130:CDS
AGATTTGAACTTCTAAGCCA+TGG	0.528702	1.4:+47183380	MS.gene066130:CDS
ATGTTATGTCAATGTGCTGA+TGG	0.528834	1.4:+47183356	MS.gene066130:CDS
ACAAGGCAAAGGAGAAAGCA+AGG	0.529082	1.4:+47183582	MS.gene066130:CDS
ATTCTTCTTATGGAGTTGAA+AGG	0.542323	1.4:+47182616	MS.gene066130:CDS
AGGGTTTGGATAAGTAAATG+AGG	0.542957	1.4:-47182717	None:intergenic
AAATCGACGGTGTATCGAAT+CGG	0.545134	1.4:+47182776	MS.gene066130:CDS
GAACTTCTAAGCCATGGATG+TGG	0.556866	1.4:+47183386	MS.gene066130:CDS
CTGATAATTGAAGATTGTAA+AGG	0.558747	1.4:-47182962	None:intergenic
TTCGATGCTTTCTCTGTCGT+AGG	0.572900	1.4:-47182457	None:intergenic
TCAAGTTGATAGTGGAAACA+AGG	0.581380	1.4:+47183565	MS.gene066130:CDS
TATTTCACTACAACTCATGA+AGG	0.582520	1.4:+47182407	MS.gene066130:CDS
CTTGCTTCATACCCAACAAG+TGG	0.584581	1.4:+47183665	MS.gene066130:CDS
CGACGGTGTATCGAATCGGC+CGG	0.591346	1.4:+47182780	MS.gene066130:CDS
TGCTAAGAACTCAACAGCGC+CGG	0.596748	1.4:-47182799	None:intergenic
CAAGGCAAAGGAGAAAGCAA+GGG	0.602241	1.4:+47183583	MS.gene066130:CDS
AATCTTCAATTATCAGAACA+AGG	0.603602	1.4:+47182969	MS.gene066130:CDS
CAATAGCTAAACCACATCCA+TGG	0.618916	1.4:-47183397	None:intergenic
TTCGTAGACTCATCCAAGCA+TGG	0.622209	1.4:+47182583	MS.gene066130:CDS
CCGTCGATTTGACTCACTCT+CGG	0.622821	1.4:-47182763	None:intergenic
TGATAGTGGAAACAAGGCAA+AGG	0.625172	1.4:+47183571	MS.gene066130:CDS
GGATGAGTCTACGAAGAGTG+TGG	0.646535	1.4:-47182575	None:intergenic
TTGCTTCATACCCAACAAGT+GGG	0.653316	1.4:+47183666	MS.gene066130:CDS
AGCACCAAAATCATCTCACA+TGG	0.654659	1.4:-47183326	None:intergenic
CCGAGAGTGAGTCAAATCGA+CGG	0.693824	1.4:+47182763	MS.gene066130:CDS
TGGCAAATGATAGAGCTGTG+AGG	0.778119	1.4:+47183450	MS.gene066130:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATGAAGCAAGTAAATTAAA+AGG	-	chr1.4:47183656-47183675	None:intergenic	20.0%
!!	TCAATGAAATTATTGCAAAA+GGG	+	chr1.4:47183038-47183057	MS.gene066130:CDS	20.0%
!!	TTCAATGAAATTATTGCAAA+AGG	+	chr1.4:47183037-47183056	MS.gene066130:CDS	20.0%
!!!	AATTGTTTTGAGCTTTTTTA+GGG	-	chr1.4:47182739-47182758	None:intergenic	20.0%
!	AATCTTCAATTATCAGAACA+AGG	+	chr1.4:47182969-47182988	MS.gene066130:CDS	25.0%
!	ACTCTCTTAAACTTCAAAAA+TGG	+	chr1.4:47182999-47183018	MS.gene066130:CDS	25.0%
!	CTGATAATTGAAGATTGTAA+AGG	-	chr1.4:47182965-47182984	None:intergenic	25.0%
!	TTAAATTGCATTCAAAGTCT+TGG	+	chr1.4:47183612-47183631	MS.gene066130:CDS	25.0%
!	TTAATTCAACTTGTCGAATT+CGG	+	chr1.4:47183224-47183243	MS.gene066130:CDS	25.0%
!	TTTGCTGAAATCTATATCAA+AGG	+	chr1.4:47183091-47183110	MS.gene066130:CDS	25.0%
!!	TGTTTCAAAGAAGATTTTGA+GGG	+	chr1.4:47183418-47183437	MS.gene066130:CDS	25.0%
!!	TTGTTTCAAAGAAGATTTTG+AGG	+	chr1.4:47183417-47183436	MS.gene066130:CDS	25.0%
!!!	CAATTGTTTTGAGCTTTTTT+AGG	-	chr1.4:47182740-47182759	None:intergenic	25.0%
!!!	TTTGAAGAGTTTTTCAATGA+GGG	-	chr1.4:47182673-47182692	None:intergenic	25.0%
!!!	TTTGCTTTTGACTTCAAAAT+AGG	+	chr1.4:47182915-47182934	MS.gene066130:CDS	25.0%
	AACTCCATAAGAAGAATTCA+TGG	-	chr1.4:47182613-47182632	None:intergenic	30.0%
	AGAGTAAAAGCAATTGAATC+AGG	+	chr1.4:47183254-47183273	MS.gene066130:CDS	30.0%
	ATTCTTCTTATGGAGTTGAA+AGG	+	chr1.4:47182616-47182635	MS.gene066130:CDS	30.0%
	TATTTCACTACAACTCATGA+AGG	+	chr1.4:47182407-47182426	MS.gene066130:CDS	30.0%
	TCATGAGTTGTAGTGAAATA+GGG	-	chr1.4:47182407-47182426	None:intergenic	30.0%
	TTCATGAGTTGTAGTGAAAT+AGG	-	chr1.4:47182408-47182427	None:intergenic	30.0%
	TTCTTCTTGATTGCAAAGAA+AGG	+	chr1.4:47183300-47183319	MS.gene066130:CDS	30.0%
	TTGATGCTTTCTTTGATATC+TGG	-	chr1.4:47183187-47183206	None:intergenic	30.0%
!!	CTTTGAAGAGTTTTTCAATG+AGG	-	chr1.4:47182674-47182693	None:intergenic	30.0%
!!	TTTGATGATGATGATGTTGA+AGG	+	chr1.4:47182891-47182910	MS.gene066130:CDS	30.0%
!!!	TTTAGATTCGCTAGTTTTGA+TGG	-	chr1.4:47183125-47183144	None:intergenic	30.0%
!!!	TTTTGAGCTTTTTTAGGGTT+TGG	-	chr1.4:47182734-47182753	None:intergenic	30.0%
	AACTAACTCGACGAAAAACT+CGG	-	chr1.4:47183150-47183169	None:intergenic	35.0%
	AGATTTGAACTTCTAAGCCA+TGG	+	chr1.4:47183380-47183399	MS.gene066130:CDS	35.0%
	AGGGTTTGGATAAGTAAATG+AGG	-	chr1.4:47182720-47182739	None:intergenic	35.0%
	ATGTTATGTCAATGTGCTGA+TGG	+	chr1.4:47183356-47183375	MS.gene066130:CDS	35.0%
	GTTCAGGAAATGTTGAGAAT+TGG	+	chr1.4:47183515-47183534	MS.gene066130:CDS	35.0%
	TGCACCATGAATTCTTCTTA+TGG	+	chr1.4:47182606-47182625	MS.gene066130:CDS	35.0%
!	CGAAAAATGGCTCTTTTCAT+CGG	+	chr1.4:47182476-47182495	MS.gene066130:CDS	35.0%
!!	ACAGAGAAAGCATCGAAAAA+TGG	+	chr1.4:47182463-47182482	MS.gene066130:CDS	35.0%
!!	GATTTTGAGGGTTTCAACAA+TGG	+	chr1.4:47183430-47183449	MS.gene066130:CDS	35.0%
!!	TCAAGTTGATAGTGGAAACA+AGG	+	chr1.4:47183565-47183584	MS.gene066130:CDS	35.0%
!!	TCAATGAGGGTTTTTGTTGT+TGG	-	chr1.4:47182660-47182679	None:intergenic	35.0%
!!	TGTTGTTGCTAAGCTTTGTT+TGG	+	chr1.4:47183538-47183557	MS.gene066130:CDS	35.0%
!!	TTGGTTCTTCAAGTTGATAG+TGG	+	chr1.4:47183557-47183576	MS.gene066130:CDS	35.0%
!!!	GTTTTTGTTGTTGGAGGTTT+TGG	-	chr1.4:47182651-47182670	None:intergenic	35.0%
!!!	TGAGATGATTTTGGTGCTTT+TGG	+	chr1.4:47183331-47183350	MS.gene066130:CDS	35.0%
!!!	TGCAACACATTTTGTTGTTC+AGG	+	chr1.4:47183499-47183518	MS.gene066130:CDS	35.0%
	AAATCGACGGTGTATCGAAT+CGG	+	chr1.4:47182776-47182795	MS.gene066130:CDS	40.0%
	AGCACCAAAATCATCTCACA+TGG	-	chr1.4:47183329-47183348	None:intergenic	40.0%
	CAATAGCTAAACCACATCCA+TGG	-	chr1.4:47183400-47183419	None:intergenic	40.0%
	GAAATAGGGCACAGAAAAAG+TGG	-	chr1.4:47182393-47182412	None:intergenic	40.0%
	GTGGTGTAACTTCGATATCT+TGG	-	chr1.4:47182374-47182393	None:intergenic	40.0%
	TTGCTTCATACCCAACAAGT+GGG	+	chr1.4:47183666-47183685	MS.gene066130:CDS	40.0%
!	GAAGCCATGTGAGATGATTT+TGG	+	chr1.4:47183322-47183341	MS.gene066130:CDS	40.0%
!!	TGATAGTGGAAACAAGGCAA+AGG	+	chr1.4:47183571-47183590	MS.gene066130:CDS	40.0%
!!!	ATGAGGGTTTTTGTTGTTGG+AGG	-	chr1.4:47182657-47182676	None:intergenic	40.0%
!!!	GTGAGTTGTTGTTTTGTGAC+AGG	-	chr1.4:47182516-47182535	None:intergenic	40.0%
	AATTCATGGTGCACCATGCT+TGG	-	chr1.4:47182599-47182618	None:intergenic	45.0%
	ACAAGGCAAAGGAGAAAGCA+AGG	+	chr1.4:47183582-47183601	MS.gene066130:CDS	45.0%
	ATGCCTGTTGAGACAGTAAC+TGG	-	chr1.4:47182435-47182454	None:intergenic	45.0%
	CAAGGCAAAGGAGAAAGCAA+GGG	+	chr1.4:47183583-47183602	MS.gene066130:CDS	45.0%
	CTTGCTTCATACCCAACAAG+TGG	+	chr1.4:47183665-47183684	MS.gene066130:CDS	45.0%
	GAACTTCTAAGCCATGGATG+TGG	+	chr1.4:47183386-47183405	MS.gene066130:CDS	45.0%
	GATCCAGTTACTGTCTCAAC+AGG	+	chr1.4:47182429-47182448	MS.gene066130:CDS	45.0%
	TGGCAAATGATAGAGCTGTG+AGG	+	chr1.4:47183450-47183469	MS.gene066130:CDS	45.0%
	TTCGATGCTTTCTCTGTCGT+AGG	-	chr1.4:47182460-47182479	None:intergenic	45.0%
	TTCGTAGACTCATCCAAGCA+TGG	+	chr1.4:47182583-47182602	MS.gene066130:CDS	45.0%
!	AGTCTACGAAGAGTGTGGTT+TGG	-	chr1.4:47182573-47182592	None:intergenic	45.0%
!!!	GTTGTTGGAGGTTTTGGAGT+TGG	-	chr1.4:47182645-47182664	None:intergenic	45.0%
	CCGAGAGTGAGTCAAATCGA+CGG	+	chr1.4:47182763-47182782	MS.gene066130:CDS	50.0%
	GGATGAGTCTACGAAGAGTG+TGG	-	chr1.4:47182578-47182597	None:intergenic	50.0%
	TGCTAAGAACTCAACAGCGC+CGG	-	chr1.4:47182802-47182821	None:intergenic	50.0%
!!	CCGTCGATTTGACTCACTCT+CGG	-	chr1.4:47182766-47182785	None:intergenic	50.0%
	CGACGGTGTATCGAATCGGC+CGG	+	chr1.4:47182780-47182799	MS.gene066130:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	47182366	47183691	47182366	ID=MS.gene066130
chr1.4	mRNA	47182366	47183691	47182366	ID=MS.gene066130.t1;Parent=MS.gene066130
chr1.4	exon	47182366	47183691	47182366	ID=MS.gene066130.t1.exon1;Parent=MS.gene066130.t1
chr1.4	CDS	47182366	47183691	47182366	ID=cds.MS.gene066130.t1;Parent=MS.gene066130.t1

Gene Sequence

>MS.gene066130

ATGAACCAAGATATCGAAGTTACACCACTTTTTCTGTGCCCTATTTCACTACAACTCATGAAGGATCCAGTTACTGTCTCAACAGGCATAACCTACGACAGAGAAAGCATCGAAAAATGGCTCTTTTCATCGGAAAACAAAACATGTCCTGTCACAAAACAACAACTCACACATGATGATGCAAATGATCTCATTATTCTCACTCCAAACCACACTCTTCGTAGACTCATCCAAGCATGGTGCACCATGAATTCTTCTTATGGAGTTGAAAGGATTCCAACTCCAAAACCTCCAACAACAAAAACCCTCATTGAAAAACTCTTCAAAGAAGCTTCTGATTCTTCAGATTCACCTCATTTACTTATCCAAACCCTAAAAAAGCTCAAAACAATTGCATCCGAGAGTGAGTCAAATCGACGGTGTATCGAATCGGCCGGCGCTGTTGAGTTCTTAGCATCAATAGTAACAAAAAACAACACAAGTTGTTCATCTTCATGTTCAGCAACAGAATTAATAGAAGTTAGCTTTGATGATGATGATGTTGAAGGTTTTGCTTTTGACTTCAAAATAGGTGCAGAAGATGAAGCTATAAACATCCTTTACAATCTTCAATTATCAGAACAAGGTTTGAAAACTCTCTTAAACTTCAAAAATGGAGAATTTTTAGACTCTTCAATGAAATTATTGCAAAAGGGTAACTATGATTCAAGAACATACGCAGTTTGTTTGCTGAAATCTATATCAAAGGTTGCTGATCCATCAAAACTAGCGAATCTAAAAACCGAGTTTTTCGTCGAGTTAGTTCAACTTCTCAAAGACCAGATATCAAAGAAAGCATCAAAAGCAACACTACAAACGTTAATTCAACTTGTCGAATTCGGAAGAAATAGAGTAAAAGCAATTGAATCAGGTTGTGTTTCTGCTTTGATAGAACTTCTTCTTGATTGCAAAGAAAGGAAGCCATGTGAGATGATTTTGGTGCTTTTGGAGATGTTATGTCAATGTGCTGATGGAAGATTTGAACTTCTAAGCCATGGATGTGGTTTAGCTATTGTTTCAAAGAAGATTTTGAGGGTTTCAACAATGGCAAATGATAGAGCTGTGAGGATTCTTCTTTCTGTTTCAAGATTTTCTGCAACACATTTTGTTGTTCAGGAAATGTTGAGAATTGGTGTTGTTGCTAAGCTTTGTTTGGTTCTTCAAGTTGATAGTGGAAACAAGGCAAAGGAGAAAGCAAGGGAGATTCTTAAATTGCATTCAAAGTCTTGGATGAATTCTCATTGCATACCTTTTAATTTACTTGCTTCATACCCAACAAGTGGGTAA

Protein sequence

>MS.gene066130.t1

MNQDIEVTPLFLCPISLQLMKDPVTVSTGITYDRESIEKWLFSSENKTCPVTKQQLTHDDANDLIILTPNHTLRRLIQAWCTMNSSYGVERIPTPKPPTTKTLIEKLFKEASDSSDSPHLLIQTLKKLKTIASESESNRRCIESAGAVEFLASIVTKNNTSCSSSCSATELIEVSFDDDDVEGFAFDFKIGAEDEAINILYNLQLSEQGLKTLLNFKNGEFLDSSMKLLQKGNYDSRTYAVCLLKSISKVADPSKLANLKTEFFVELVQLLKDQISKKASKATLQTLIQLVEFGRNRVKAIESGCVSALIELLLDCKERKPCEMILVLLEMLCQCADGRFELLSHGCGLAIVSKKILRVSTMANDRAVRILLSVSRFSATHFVVQEMLRIGVVAKLCLVLQVDSGNKAKEKAREILKLHSKSWMNSHCIPFNLLASYPTSG