AlfalfaGEDB | MS.gene26489

M. sativa cultivar XinJiangDaYe / MS.gene26489

KEGG: -
GO: GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0070647 GO:0071704 GO:0140096 GO:1901564
PFAM: PF04564.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26489.t1	XP_013450220.1	98.8	421	4	1	1	420	1	421	1.30E-228	802

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26489.t1	Q9SVC6	60.1	436	146	7	5	417	4	434	8.5e-139	495.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26489.t1	A0A072U521	98.8	421	4	1	1	420	1	421	9.7e-229	802.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05015	MS.gene26489	0.803221	3.87E-49	-1.69E-46
MS.gene051837	MS.gene26489	0.81525	1.02E-51	-1.69E-46
MS.gene051854	MS.gene26489	0.815467	9.11E-52	-1.69E-46
MS.gene051959	MS.gene26489	0.805781	1.13E-49	-1.69E-46
MS.gene051960	MS.gene26489	0.802375	5.78E-49	-1.69E-46
MS.gene055549	MS.gene26489	0.820312	7.33E-53	-1.69E-46
MS.gene055550	MS.gene26489	0.8273	1.69E-54	-1.69E-46
MS.gene057093	MS.gene26489	0.803335	3.66E-49	-1.69E-46
MS.gene057167	MS.gene26489	0.814685	1.36E-51	-1.69E-46
MS.gene058628	MS.gene26489	0.802326	5.92E-49	-1.69E-46
MS.gene06061	MS.gene26489	0.841163	5.61E-58	-1.69E-46
MS.gene060964	MS.gene26489	0.802436	5.62E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene41036	MS.gene26489	PPI
MS.gene26489	MS.gene72611	PPI
MS.gene066125	MS.gene26489	PPI
MS.gene022190	MS.gene26489	PPI
MS.gene034756	MS.gene26489	PPI
MS.gene061546	MS.gene26489	PPI
MS.gene064492	MS.gene26489	PPI
MS.gene017373	MS.gene26489	PPI
MS.gene26489	MS.gene071765	PPI
MS.gene042912	MS.gene26489	PPI
MS.gene93972	MS.gene26489	PPI
MS.gene048677	MS.gene26489	PPI
MS.gene41035	MS.gene26489	PPI
MS.gene008306	MS.gene26489	PPI
MS.gene04998	MS.gene26489	PPI
MS.gene26139	MS.gene26489	PPI
MS.gene061543	MS.gene26489	PPI
MS.gene26489	MS.gene41211	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26489.t1	MTR_7g106340	98.812	421	4	1	1	420	1	421	0.0	851
MS.gene26489.t1	MTR_1g056880	64.531	437	132	5	1	417	1	434	0.0	543
MS.gene26489.t1	MTR_1g056870	63.844	437	135	5	1	417	1	434	0.0	534
MS.gene26489.t1	MTR_1g056840	60.870	437	122	7	1	417	1	408	4.71e-177	500
MS.gene26489.t1	MTR_2g087350	58.413	416	157	5	4	417	2	403	4.63e-159	454
MS.gene26489.t1	MTR_1g056910	43.510	416	219	6	4	415	2	405	1.79e-111	333
MS.gene26489.t1	MTR_4g051515	40.235	425	225	10	5	416	3	411	1.04e-94	291
MS.gene26489.t1	MTR_3g065080	34.360	422	251	11	4	416	32	436	1.47e-59	200
MS.gene26489.t1	MTR_5g077510	32.941	425	246	13	5	413	32	433	1.01e-55	190
MS.gene26489.t1	MTR_4g085720	33.086	405	240	14	6	402	12	393	1.12e-49	173
MS.gene26489.t1	MTR_6g071340	30.120	415	247	11	5	397	2	395	2.88e-49	172
MS.gene26489.t1	MTR_5g048050	31.090	431	264	14	5	416	22	438	2.14e-48	171
MS.gene26489.t1	MTR_8g092870	31.592	402	252	11	6	397	8	396	9.53e-41	150
MS.gene26489.t1	MTR_3g095730	31.990	397	255	11	6	397	11	397	1.76e-39	146
MS.gene26489.t1	MTR_3g096370	30.147	408	258	13	5	402	7	397	1.67e-37	140
MS.gene26489.t1	MTR_4g063800	29.440	411	247	12	2	400	249	628	1.65e-35	139
MS.gene26489.t1	MTR_5g015210	27.569	399	263	8	7	405	66	438	1.94e-32	127
MS.gene26489.t1	MTR_4g485520	29.642	307	177	11	4	296	101	382	1.84e-26	111
MS.gene26489.t1	MTR_4g485520	29.642	307	177	11	4	296	269	550	2.79e-26	111
MS.gene26489.t1	MTR_7g059405	26.121	379	236	10	8	370	264	614	2.81e-26	111
MS.gene26489.t1	MTR_1g017770	27.962	422	257	12	7	415	44	431	5.57e-26	109
MS.gene26489.t1	MTR_7g078330	30.952	294	165	10	8	291	274	539	1.20e-25	109
MS.gene26489.t1	MTR_2g018010	27.036	307	178	9	6	291	290	571	1.91e-24	106
MS.gene26489.t1	MTR_1g094215	30.137	292	175	8	8	291	64	334	3.65e-24	104
MS.gene26489.t1	MTR_3g078160	30.618	356	216	16	9	353	276	611	4.13e-24	105
MS.gene26489.t1	MTR_3g078160	30.726	358	213	16	9	353	276	611	4.64e-24	105
MS.gene26489.t1	MTR_1g094215	30.137	292	175	8	8	291	247	517	9.59e-24	103
MS.gene26489.t1	MTR_4g107010	28.750	400	256	15	9	397	281	662	1.02e-23	104
MS.gene26489.t1	MTR_5g083030	26.393	341	202	11	6	321	291	607	1.07e-23	103
MS.gene26489.t1	MTR_5g034440	28.226	372	231	15	12	368	284	634	3.38e-22	99.8
MS.gene26489.t1	MTR_4g091880	30.149	335	197	10	8	337	5	307	1.43e-20	93.2
MS.gene26489.t1	MTR_4g028960	28.358	268	152	10	5	251	277	525	1.57e-20	94.4
MS.gene26489.t1	MTR_4g091880	29.091	330	179	9	8	337	5	279	1.80e-20	92.4
MS.gene26489.t1	MTR_3g078160	33.200	250	144	11	9	250	276	510	1.67e-19	91.3
MS.gene26489.t1	MTR_2g011140	27.178	287	182	8	14	295	21	285	7.31e-18	85.1
MS.gene26489.t1	MTR_3g085610	44.186	86	40	3	9	94	281	358	2.05e-16	82.0
MS.gene26489.t1	MTR_7g053260	48.148	81	37	3	6	86	76	151	2.19e-16	81.3
MS.gene26489.t1	MTR_7g116600	48.864	88	39	4	4	91	74	155	3.08e-16	80.9
MS.gene26489.t1	MTR_1g069845	47.945	73	34	3	6	77	122	191	3.53e-16	80.9
MS.gene26489.t1	MTR_1g069845	47.945	73	34	3	6	77	243	312	6.38e-16	80.5
MS.gene26489.t1	MTR_1g069845	47.222	72	36	2	6	77	239	308	6.84e-16	80.5
MS.gene26489.t1	MTR_8g103227	36.441	118	66	2	9	118	396	512	1.70e-15	79.3
MS.gene26489.t1	MTR_7g097020	45.333	75	39	2	6	80	239	311	1.59e-14	76.3
MS.gene26489.t1	MTR_8g080280	23.831	449	272	15	9	404	283	714	1.69e-14	75.9
MS.gene26489.t1	MTR_8g077205	26.720	378	216	19	9	375	164	491	8.77e-14	73.6
MS.gene26489.t1	MTR_8g077205	26.720	378	216	19	9	375	276	603	1.31e-13	73.2
MS.gene26489.t1	MTR_3g115670	28.767	146	85	4	12	153	34	164	1.62e-13	73.2
MS.gene26489.t1	MTR_1g090320	50.000	68	33	1	9	76	514	580	2.57e-13	72.8
MS.gene26489.t1	MTR_1g090320	50.000	68	33	1	9	76	516	582	2.62e-13	72.8
MS.gene26489.t1	MTR_3g466220	46.053	76	37	3	6	80	231	303	3.13e-13	72.0
MS.gene26489.t1	MTR_3g466220	46.053	76	37	3	6	80	210	282	3.18e-13	72.0
MS.gene26489.t1	MTR_1g079450	38.235	102	57	4	1	102	56	151	4.00e-13	71.2
MS.gene26489.t1	MTR_5g032010	45.714	70	36	2	7	76	731	798	6.37e-13	71.2
MS.gene26489.t1	MTR_2g096850	46.575	73	37	2	4	76	585	655	3.17e-12	68.9
MS.gene26489.t1	MTR_2g096850	46.575	73	37	2	4	76	736	806	3.22e-12	68.9
MS.gene26489.t1	MTR_5g020570	40.909	88	46	4	4	91	269	350	3.98e-12	68.6
MS.gene26489.t1	MTR_5g020570	40.909	88	46	4	4	91	284	365	4.75e-12	68.6
MS.gene26489.t1	MTR_1g093965	52.459	61	27	2	21	81	1	59	5.36e-12	65.1
MS.gene26489.t1	MTR_1g100820	46.479	71	36	2	9	79	649	717	8.09e-12	67.8
MS.gene26489.t1	MTR_1g100820	46.479	71	36	2	9	79	647	715	8.37e-12	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26489.t1	AT3G52450	59.361	438	153	7	1	417	1	434	0.0	511
MS.gene26489.t1	AT2G35930	60.000	420	150	7	1	417	6	410	7.23e-177	500
MS.gene26489.t1	AT3G11840	37.472	451	234	7	2	414	4	444	6.53e-89	277
MS.gene26489.t1	AT3G19380	35.385	390	226	8	6	386	12	384	7.51e-62	206
MS.gene26489.t1	AT1G49780	33.735	415	251	10	6	411	12	411	2.09e-56	191
MS.gene26489.t1	AT5G37490	30.673	401	256	12	3	397	26	410	2.91e-46	165
MS.gene26489.t1	AT1G66160	28.537	417	231	10	5	397	30	403	3.74e-46	165
MS.gene26489.t1	AT3G18710	29.902	408	261	12	1	397	1	394	2.06e-33	130
MS.gene26489.t1	AT1G66160	25.899	417	226	11	5	397	30	387	2.23e-33	130
MS.gene26489.t1	AT5G09800	28.101	395	261	9	8	397	11	387	2.54e-33	129
MS.gene26489.t1	AT1G23030	29.808	312	184	10	4	307	237	521	1.36e-31	127
MS.gene26489.t1	AT1G71020	28.750	320	186	9	4	307	91	384	3.11e-30	122
MS.gene26489.t1	AT1G71020	28.750	320	186	9	4	307	239	532	7.50e-30	122
MS.gene26489.t1	AT5G65920	28.040	403	256	10	7	405	59	431	1.37e-29	119
MS.gene26489.t1	AT5G65920	28.040	403	256	10	7	405	59	431	1.37e-29	119
MS.gene26489.t1	AT3G46510	28.467	411	239	13	8	400	256	629	1.38e-29	121
MS.gene26489.t1	AT5G64660	27.737	411	260	13	6	398	8	399	4.03e-29	118
MS.gene26489.t1	AT3G49810	27.157	394	255	9	7	397	63	427	5.22e-29	118
MS.gene26489.t1	AT5G42340	30.961	281	166	9	8	284	290	546	7.12e-27	113
MS.gene26489.t1	AT2G28830	30.744	309	172	11	2	293	178	461	4.28e-26	110
MS.gene26489.t1	AT3G54850	28.328	293	180	8	8	291	248	519	5.11e-26	110
MS.gene26489.t1	AT1G29340	26.570	414	256	14	6	400	303	687	1.34e-24	107
MS.gene26489.t1	AT4G21350	29.326	341	205	11	6	341	3	312	8.21e-23	99.8
MS.gene26489.t1	AT1G10560	26.979	341	213	11	2	326	280	600	5.43e-22	99.0
MS.gene26489.t1	AT5G01830	28.276	290	175	9	5	284	271	537	7.96e-20	92.4
MS.gene26489.t1	AT1G60190	26.829	328	204	12	14	326	284	590	2.06e-18	88.2
MS.gene26489.t1	AT3G02840	26.377	345	223	13	85	412	40	370	3.08e-17	83.2
MS.gene26489.t1	AT3G07360	27.027	296	180	10	6	287	72	345	8.72e-17	82.4
MS.gene26489.t1	AT1G67530	47.059	85	39	3	9	93	273	351	5.21e-16	80.5
MS.gene26489.t1	AT5G18320	29.617	287	182	8	2	284	66	336	6.15e-16	80.1
MS.gene26489.t1	AT1G67530	47.059	85	39	3	9	93	273	351	6.90e-16	80.5
MS.gene26489.t1	AT1G67530	47.059	85	39	3	9	93	273	351	6.90e-16	80.5
MS.gene26489.t1	AT1G67530	47.059	85	39	3	9	93	273	351	6.90e-16	80.5
MS.gene26489.t1	AT1G27910	47.059	85	39	3	9	93	280	358	3.18e-15	78.2
MS.gene26489.t1	AT5G65500	46.914	81	41	2	4	84	719	797	3.67e-15	78.2
MS.gene26489.t1	AT5G65500	46.914	81	41	2	4	84	711	789	4.07e-15	78.2
MS.gene26489.t1	AT1G24330	47.059	85	39	4	9	93	276	354	8.49e-15	77.0
MS.gene26489.t1	AT1G24330	47.059	85	39	4	9	93	276	354	8.49e-15	77.0
MS.gene26489.t1	AT5G18340	27.653	311	189	12	2	299	68	355	1.35e-14	75.9
MS.gene26489.t1	AT3G54790	44.000	75	40	2	6	80	200	272	9.91e-14	73.6
MS.gene26489.t1	AT5G67340	46.667	75	38	2	6	80	238	310	1.01e-13	73.6
MS.gene26489.t1	AT5G67340	46.667	75	38	2	6	80	238	310	1.01e-13	73.6
MS.gene26489.t1	AT1G01660	42.466	73	40	2	9	81	499	569	1.05e-13	73.2
MS.gene26489.t1	AT1G01660	42.466	73	40	2	9	81	498	568	1.07e-13	73.2
MS.gene26489.t1	AT3G54790	44.000	75	40	2	6	80	236	308	1.25e-13	73.6
MS.gene26489.t1	AT3G54790	44.000	75	40	2	6	80	236	308	1.25e-13	73.6
MS.gene26489.t1	AT2G45920	44.737	76	40	2	7	82	324	397	2.28e-13	71.6
MS.gene26489.t1	AT5G57035	43.478	69	37	2	8	76	708	774	4.06e-13	71.6
MS.gene26489.t1	AT2G23140	37.634	93	52	3	12	104	234	320	8.41e-13	70.9
MS.gene26489.t1	AT2G23140	37.634	93	52	3	12	104	234	320	8.41e-13	70.9
MS.gene26489.t1	AT2G23140	37.634	93	52	3	12	104	237	323	9.12e-13	70.9
MS.gene26489.t1	AT1G68940	27.564	156	82	4	12	158	303	436	9.21e-13	70.9
MS.gene26489.t1	AT1G68940	27.564	156	82	4	12	158	250	383	9.38e-13	70.9
MS.gene26489.t1	AT2G23140	37.634	93	52	3	12	104	45	131	9.39e-13	70.5
MS.gene26489.t1	AT2G23140	37.634	93	52	3	12	104	45	131	9.39e-13	70.5
MS.gene26489.t1	AT1G68940	27.564	156	82	4	12	158	303	436	9.62e-13	70.9
MS.gene26489.t1	AT4G36550	43.243	74	40	2	8	81	219	290	1.36e-12	70.1
MS.gene26489.t1	AT2G19410	45.070	71	36	2	6	76	709	776	3.10e-12	68.9
MS.gene26489.t1	AT2G19410	45.070	71	36	2	6	76	723	790	3.50e-12	68.9
MS.gene26489.t1	AT2G19410	45.070	71	36	2	6	76	744	811	3.74e-12	68.9
MS.gene26489.t1	AT2G45910	44.928	69	36	2	8	76	763	829	6.38e-12	68.2
MS.gene26489.t1	AT2G45910	44.928	69	36	2	8	76	763	829	6.38e-12	68.2
MS.gene26489.t1	AT5G18330	27.513	378	242	16	2	372	59	411	6.46e-12	67.4
MS.gene26489.t1	AT3G61390	41.096	73	41	2	7	79	277	347	6.63e-12	67.0
MS.gene26489.t1	AT3G61390	41.096	73	41	2	7	79	352	422	7.24e-12	67.4
MS.gene26489.t1	AT1G76390	42.029	69	35	2	12	76	29	96	1.16e-11	67.4
MS.gene26489.t1	AT1G76390	42.029	69	35	2	12	76	29	96	1.16e-11	67.4
MS.gene26489.t1	AT1G01680	46.377	69	35	2	12	80	237	303	1.45e-11	65.5
MS.gene26489.t1	AT1G20780	41.096	73	38	2	12	80	27	98	2.46e-11	66.2
MS.gene26489.t1	AT1G20780	41.096	73	38	2	12	80	27	98	2.46e-11	66.2
MS.gene26489.t1	AT1G20780	41.096	73	38	2	12	80	27	98	2.46e-11	66.2

Find 53 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAATTAATTTCACTCATAAT+TGG	0.132240	7.3:+7824001	None:intergenic
CTTCATGATTTCTAATGATA+TGG	0.167930	7.3:+7825207	None:intergenic
AATTGAGTTGTTCGTTGAAT+TGG	0.278950	7.3:-7824537	MS.gene26489:CDS
TTGTTTCTAAGAAGATTCTA+AGG	0.280230	7.3:-7824262	MS.gene26489:CDS
AATTAATTTCACTCATAATT+GGG	0.308217	7.3:+7824002	None:intergenic
AGACTTAGATCTATGGCTTC+TGG	0.311800	7.3:-7824890	MS.gene26489:CDS
ATACCTGTTGAGACTGTTAC+TGG	0.321164	7.3:+7825181	None:intergenic
CCCATGGACAGAGTGCAATT+AGG	0.328586	7.3:+7824459	None:intergenic
AGCATTTCTTGAACAACATT+AGG	0.349387	7.3:+7824173	None:intergenic
AAAACCAACATCATTTCATT+TGG	0.354293	7.3:+7824353	None:intergenic
CCTGAAAAGACTTAGATCTA+TGG	0.371986	7.3:-7824897	MS.gene26489:CDS
TTCATGATTTCTAATGATAT+GGG	0.374449	7.3:+7825208	None:intergenic
CCATGGACAGAGTGCAATTA+GGG	0.383112	7.3:+7824460	None:intergenic
AACAAAAGTTCTATTAAAAC+AGG	0.395977	7.3:+7824398	None:intergenic
CCCTAATTGCACTCTGTCCA+TGG	0.442705	7.3:-7824460	MS.gene26489:CDS
ATTGACTTTGTTAACAACTC+TGG	0.442706	7.3:-7824785	MS.gene26489:CDS
TTGATTCTGTTTCTTCCCCA+TGG	0.450469	7.3:+7824443	None:intergenic
TAACAAAAGTTATGCAGAAA+GGG	0.457802	7.3:-7824643	MS.gene26489:CDS
AATCTTCATGTGTCTGAAGC+TGG	0.462024	7.3:-7824713	MS.gene26489:CDS
TGTTGTGGCTAAGCTTTGCT+TGG	0.464959	7.3:-7824141	MS.gene26489:CDS
TTAACAAAAGTTATGCAGAA+AGG	0.481783	7.3:-7824644	MS.gene26489:CDS
AGAATCAAAGGTGTTGAAGC+TGG	0.489133	7.3:-7824428	MS.gene26489:CDS
GATCCAGTAACAGTCTCAAC+AGG	0.492806	7.3:-7825184	MS.gene26489:CDS
AGATACTTAAGATGCATGCT+AGG	0.498217	7.3:-7824073	MS.gene26489:CDS
AAACTACTAACTTTCAGAAA+CGG	0.507085	7.3:-7824683	MS.gene26489:CDS
AATCTTTGTCAGTGTGCTGA+AGG	0.523947	7.3:-7824323	MS.gene26489:CDS
ACGCTTCACAAGGCATTGAA+AGG	0.524077	7.3:-7825012	MS.gene26489:CDS
CCTAATTGCACTCTGTCCAT+GGG	0.527316	7.3:-7824459	MS.gene26489:CDS
TTAGAAGACTCATTCAAGCT+TGG	0.528792	7.3:-7825045	MS.gene26489:CDS
AAAACCAAATGAAATGATGT+TGG	0.529378	7.3:-7824357	MS.gene26489:CDS
TATGAAGAAAGCAAATTACT+AGG	0.534963	7.3:+7824026	None:intergenic
AGCAAATTACTAGGTATGCA+TGG	0.539218	7.3:+7824035	None:intergenic
ATTAATTTCACTCATAATTG+GGG	0.541983	7.3:+7824003	None:intergenic
GTTCAAGAAATGCTTAAACT+TGG	0.545979	7.3:-7824164	MS.gene26489:CDS
GAATGAGTCTTCTAAGAGTG+TGG	0.559382	7.3:+7825053	None:intergenic
TGGGGAAGAAACAGAATCAA+AGG	0.566142	7.3:-7824440	MS.gene26489:CDS
AGTCTTCTAAGAGTGTGGTT+TGG	0.569998	7.3:+7825058	None:intergenic
TGATTGTGGAAGTAAGACTA+AGG	0.572296	7.3:-7824108	MS.gene26489:CDS
TTAAGATGCATGCTAGGGCA+TGG	0.573333	7.3:-7824067	MS.gene26489:CDS
TGTTTCTAAGAAGATTCTAA+GGG	0.577022	7.3:-7824261	MS.gene26489:CDS
TTCAATACTTTCTCTGTCGT+AGG	0.588046	7.3:+7825156	None:intergenic
AAACGTTGCATAGAAGATGC+AGG	0.588793	7.3:-7824854	MS.gene26489:CDS
ATGGGGCAGACAAAGAATGA+AGG	0.611200	7.3:+7825226	None:intergenic
CATATCATTAGAAATCATGA+AGG	0.612177	7.3:-7825206	MS.gene26489:CDS
TCATGATTTCTAATGATATG+GGG	0.613195	7.3:+7825209	None:intergenic
AATGCTTAAACTTGGTGTTG+TGG	0.614712	7.3:-7824156	MS.gene26489:CDS
GATACTTAAGATGCATGCTA+GGG	0.623745	7.3:-7824072	MS.gene26489:CDS
TGTACATTAAACGCTTCACA+AGG	0.640683	7.3:-7825022	MS.gene26489:CDS
TTGGTTCTTCAAGTTGATTG+TGG	0.650079	7.3:-7824122	MS.gene26489:CDS
TATGGATCATGATGAGATCG+AGG	0.658015	7.3:-7825251	MS.gene26489:CDS
CTAATTGCACTCTGTCCATG+GGG	0.676561	7.3:-7824458	MS.gene26489:CDS
AGATGCATGCTAGGGCATGG+AGG	0.701659	7.3:-7824064	MS.gene26489:CDS
TTGCGAATGATAGAGCTGTG+AGG	0.775637	7.3:-7824229	MS.gene26489:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACAAAAGTTCTATTAAAAC+AGG	+	chr7.3:7824865-7824884	None:intergenic	20.0%
!!	TTCATGATTTCTAATGATAT+GGG	+	chr7.3:7824055-7824074	None:intergenic	20.0%
!	AAAACCAAATGAAATGATGT+TGG	-	chr7.3:7824903-7824922	MS.gene26489:CDS	25.0%
!	AAAACCAACATCATTTCATT+TGG	+	chr7.3:7824910-7824929	None:intergenic	25.0%
!	AAACTACTAACTTTCAGAAA+CGG	-	chr7.3:7824577-7824596	MS.gene26489:CDS	25.0%
!	CATATCATTAGAAATCATGA+AGG	-	chr7.3:7824054-7824073	MS.gene26489:CDS	25.0%
!	CTTCATGATTTCTAATGATA+TGG	+	chr7.3:7824056-7824075	None:intergenic	25.0%
!	TAACAAAAGTTATGCAGAAA+GGG	-	chr7.3:7824617-7824636	MS.gene26489:CDS	25.0%
!	TATGAAGAAAGCAAATTACT+AGG	+	chr7.3:7825237-7825256	None:intergenic	25.0%
!	TCATGATTTCTAATGATATG+GGG	+	chr7.3:7824054-7824073	None:intergenic	25.0%
!	TTAACAAAAGTTATGCAGAA+AGG	-	chr7.3:7824616-7824635	MS.gene26489:CDS	25.0%
!!	TGTTTCTAAGAAGATTCTAA+GGG	-	chr7.3:7824999-7825018	MS.gene26489:CDS	25.0%
!!!	ACAGAGAAAGTATTGAAAAA+TGG	-	chr7.3:7824110-7824129	MS.gene26489:CDS	25.0%
!!!	TTGTTTCTAAGAAGATTCTA+AGG	-	chr7.3:7824998-7825017	MS.gene26489:CDS	25.0%
!!!	TTTGATGAGTTTTGTGATTT+GGG	+	chr7.3:7824305-7824324	None:intergenic	25.0%
	AGCATTTCTTGAACAACATT+AGG	+	chr7.3:7825090-7825109	None:intergenic	30.0%
	GTTCAAGAAATGCTTAAACT+TGG	-	chr7.3:7825096-7825115	MS.gene26489:CDS	30.0%
	TAAGACTAAGGAAAAAGCAA+GGG	-	chr7.3:7825164-7825183	MS.gene26489:CDS	30.0%
!	AATTGAGTTGTTCGTTGAAT+TGG	-	chr7.3:7824723-7824742	MS.gene26489:CDS	30.0%
!	ATTGACTTTGTTAACAACTC+TGG	-	chr7.3:7824475-7824494	MS.gene26489:CDS	30.0%
!!	CTTTGATGAGTTTTGTGATT+TGG	+	chr7.3:7824306-7824325	None:intergenic	30.0%
!!	TGAATTGGTACAAGTTTTGA+AGG	-	chr7.3:7824738-7824757	MS.gene26489:CDS	30.0%
!!!	GATAGCTGTTGTTTTGTAAT+AGG	+	chr7.3:7824163-7824182	None:intergenic	30.0%
!!!	TGAAATGATGTTGGTTTTGT+TGG	-	chr7.3:7824912-7824931	MS.gene26489:CDS	30.0%
	AGATACTTAAGATGCATGCT+AGG	-	chr7.3:7825187-7825206	MS.gene26489:CDS	35.0%
	AGCAAATTACTAGGTATGCA+TGG	+	chr7.3:7825228-7825247	None:intergenic	35.0%
	CCTGAAAAGACTTAGATCTA+TGG	-	chr7.3:7824363-7824382	MS.gene26489:CDS	35.0%
	GATACTTAAGATGCATGCTA+GGG	-	chr7.3:7825188-7825207	MS.gene26489:CDS	35.0%
	GTAAGACTAAGGAAAAAGCA+AGG	-	chr7.3:7825163-7825182	MS.gene26489:CDS	35.0%
	TGATTGTGGAAGTAAGACTA+AGG	-	chr7.3:7825152-7825171	MS.gene26489:CDS	35.0%
	TGTACATTAAACGCTTCACA+AGG	-	chr7.3:7824238-7824257	MS.gene26489:CDS	35.0%
	TTAGAAGACTCATTCAAGCT+TGG	-	chr7.3:7824215-7824234	MS.gene26489:CDS	35.0%
	TTCAATACTTTCTCTGTCGT+AGG	+	chr7.3:7824107-7824126	None:intergenic	35.0%
!	CCATAGATCTAAGTCTTTTC+AGG	+	chr7.3:7824366-7824385	None:intergenic	35.0%
!	TTTTGATGTAGTAACTGCAC+TGG	+	chr7.3:7824703-7824722	None:intergenic	35.0%
!!	AATGCTTAAACTTGGTGTTG+TGG	-	chr7.3:7825104-7825123	MS.gene26489:CDS	35.0%
!!	GTTTTGTTGATTGGTGGTTT+TGG	+	chr7.3:7824283-7824302	None:intergenic	35.0%
!!	TTGGTTCTTCAAGTTGATTG+TGG	-	chr7.3:7825138-7825157	MS.gene26489:CDS	35.0%
!!!	ATTTGGGTTTTGTTGATTGG+TGG	+	chr7.3:7824289-7824308	None:intergenic	35.0%
!!!	GTGATTTGGGTTTTGTTGAT+TGG	+	chr7.3:7824292-7824311	None:intergenic	35.0%
	AAACGTTGCATAGAAGATGC+AGG	-	chr7.3:7824406-7824425	MS.gene26489:CDS	40.0%
	AATCTTTGTCAGTGTGCTGA+AGG	-	chr7.3:7824937-7824956	MS.gene26489:CDS	40.0%
	AGACTTAGATCTATGGCTTC+TGG	-	chr7.3:7824370-7824389	MS.gene26489:CDS	40.0%
	ATACCTGTTGAGACTGTTAC+TGG	+	chr7.3:7824082-7824101	None:intergenic	40.0%
	TGGGGAAGAAACAGAATCAA+AGG	-	chr7.3:7824820-7824839	MS.gene26489:CDS	40.0%
!	AATCTTCATGTGTCTGAAGC+TGG	-	chr7.3:7824547-7824566	MS.gene26489:CDS	40.0%
!	AGTCTTCTAAGAGTGTGGTT+TGG	+	chr7.3:7824205-7824224	None:intergenic	40.0%
!	GAATGAGTCTTCTAAGAGTG+TGG	+	chr7.3:7824210-7824229	None:intergenic	40.0%
!	TTGATTCTGTTTCTTCCCCA+TGG	+	chr7.3:7824820-7824839	None:intergenic	40.0%
!!	AGAATCAAAGGTGTTGAAGC+TGG	-	chr7.3:7824832-7824851	MS.gene26489:CDS	40.0%
!!!	TTGATTGGTGGTTTTGGAGT+TGG	+	chr7.3:7824277-7824296	None:intergenic	40.0%
	ATGGGGCAGACAAAGAATGA+AGG	+	chr7.3:7824037-7824056	None:intergenic	45.0%
	CCATGGACAGAGTGCAATTA+GGG	+	chr7.3:7824803-7824822	None:intergenic	45.0%
	CCTAATTGCACTCTGTCCAT+GGG	-	chr7.3:7824801-7824820	MS.gene26489:CDS	45.0%
	CTAATTGCACTCTGTCCATG+GGG	-	chr7.3:7824802-7824821	MS.gene26489:CDS	45.0%
	GATCCAGTAACAGTCTCAAC+AGG	-	chr7.3:7824076-7824095	MS.gene26489:CDS	45.0%
	TGTTGTGGCTAAGCTTTGCT+TGG	-	chr7.3:7825119-7825138	MS.gene26489:CDS	45.0%
	TTAAGATGCATGCTAGGGCA+TGG	-	chr7.3:7825193-7825212	MS.gene26489:CDS	45.0%
	TTGCGAATGATAGAGCTGTG+AGG	-	chr7.3:7825031-7825050	MS.gene26489:CDS	45.0%
!!	ACGCTTCACAAGGCATTGAA+AGG	-	chr7.3:7824248-7824267	MS.gene26489:CDS	45.0%
!!!	GAACTTTTGAGACATGCAGC+AGG	-	chr7.3:7824967-7824986	MS.gene26489:CDS	45.0%
	CCCATGGACAGAGTGCAATT+AGG	+	chr7.3:7824804-7824823	None:intergenic	50.0%
	CCCTAATTGCACTCTGTCCA+TGG	-	chr7.3:7824800-7824819	MS.gene26489:CDS	50.0%
	AGATGCATGCTAGGGCATGG+AGG	-	chr7.3:7825196-7825215	MS.gene26489:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	7824010	7825272	7824010	ID=MS.gene26489
chr7.3	mRNA	7824010	7825272	7824010	ID=MS.gene26489.t1;Parent=MS.gene26489
chr7.3	exon	7824010	7825272	7824010	ID=MS.gene26489.t1.exon1;Parent=MS.gene26489.t1
chr7.3	CDS	7824010	7825272	7824010	ID=cds.MS.gene26489.t1;Parent=MS.gene26489.t1

Gene Sequence

>MS.gene26489

ATGGATCATGATGAGATCGAGGTTCCTTCATTCTTTGTCTGCCCCATATCATTAGAAATCATGAAGGATCCAGTAACAGTCTCAACAGGTATAACCTACGACAGAGAAAGTATTGAAAAATGGCTATTTTCGAAGAAAAACATGACATGTCCTATTACAAAACAACAGCTATCAGATTACACTGATCTAACACCAAACCACACTCTTAGAAGACTCATTCAAGCTTGGTGTACATTAAACGCTTCACAAGGCATTGAAAGGATTCCAACTCCAAAACCACCAATCAACAAAACCCAAATCACAAAACTCATCAAAGAAGCTTCTCATTCATCTCTCACTGTGCAGCTTAAATGCCTGAAAAGACTTAGATCTATGGCTTCTGGAAGTGAAACAAACAAACGTTGCATAGAAGATGCAGGTGCTGTTGAGTTTATAGCTTCTATTGTAATAAACAATAGTTGCAACATTGACTTTGTTAACAACTCTGGATCTTTATCAGAGACTAACACTGTTGATGAAGCATTAAGCATACTTCATAATCTTCATGTGTCTGAAGCTGGATTGAAGAAACTACTAACTTTCAGAAACGGCGAATTCATTGAGTCATTAACAAAAGTTATGCAGAAAGGGTTCTTTGAGTCAAGAGCTTATGCAGTTTTCTTATTAAAATCTATGTCAGAAGTAGCTGAACCAGTGCAGTTACTACATCAAAAAATTGAGTTGTTCGTTGAATTGGTACAAGTTTTGAAGGATCAAATATCACAGAAAGTATCAAAAGCAGCACTTCAGACCCTAATTGCACTCTGTCCATGGGGAAGAAACAGAATCAAAGGTGTTGAAGCTGGAACAGTTCCTGTTTTAATAGAACTTTTGTTAGAAAATTGCAAAGACAGAAAACCAAATGAAATGATGTTGGTTTTGTTGGAGAATCTTTGTCAGTGTGCTGAAGGAAGAGCTGAACTTTTGAGACATGCAGCAGGTCTTGCTGTTGTTTCTAAGAAGATTCTAAGGGTTTCGACGCTTGCGAATGATAGAGCTGTGAGGATTCTGTTGTCTGTTTCAAGATTCTCTGCAACTCCTAATGTTGTTCAAGAAATGCTTAAACTTGGTGTTGTGGCTAAGCTTTGCTTGGTTCTTCAAGTTGATTGTGGAAGTAAGACTAAGGAAAAAGCAAGGGAGATACTTAAGATGCATGCTAGGGCATGGAGGAATTCTCCATGCATACCTAGTAATTTGCTTTCTTCATACCCCAATTATGAGTGA

Protein sequence

>MS.gene26489.t1

MDHDEIEVPSFFVCPISLEIMKDPVTVSTGITYDRESIEKWLFSKKNMTCPITKQQLSDYTDLTPNHTLRRLIQAWCTLNASQGIERIPTPKPPINKTQITKLIKEASHSSLTVQLKCLKRLRSMASGSETNKRCIEDAGAVEFIASIVINNSCNIDFVNNSGSLSETNTVDEALSILHNLHVSEAGLKKLLTFRNGEFIESLTKVMQKGFFESRAYAVFLLKSMSEVAEPVQLLHQKIELFVELVQVLKDQISQKVSKAALQTLIALCPWGRNRIKGVEAGTVPVLIELLLENCKDRKPNEMMLVLLENLCQCAEGRAELLRHAAGLAVVSKKILRVSTLANDRAVRILLSVSRFSATPNVVQEMLKLGVVAKLCLVLQVDCGSKTKEKAREILKMHARAWRNSPCIPSNLLSSYPNYE