Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41217.t1 | XP_013467320.1 | 93.9 | 231 | 7 | 2 | 1 | 231 | 153 | 376 | 6.90E-116 | 426.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41217.t1 | A0A072VHW2 | 93.9 | 231 | 7 | 2 | 1 | 231 | 153 | 376 | 5.0e-116 | 426.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049149 | MS.gene41217 | 0.811417 | 7.08E-51 | -1.69E-46 |
| MS.gene049151 | MS.gene41217 | 0.814699 | 1.35E-51 | -1.69E-46 |
| MS.gene049153 | MS.gene41217 | 0.819386 | 1.19E-52 | -1.69E-46 |
| MS.gene050227 | MS.gene41217 | 0.800596 | 1.34E-48 | -1.69E-46 |
| MS.gene050426 | MS.gene41217 | -0.844527 | 7.15E-59 | -1.69E-46 |
| MS.gene050623 | MS.gene41217 | -0.81739 | 3.38E-52 | -1.69E-46 |
| MS.gene050642 | MS.gene41217 | 0.878198 | 3.41E-69 | -1.69E-46 |
| MS.gene051860 | MS.gene41217 | 0.816317 | 5.89E-52 | -1.69E-46 |
| MS.gene051983 | MS.gene41217 | 0.869927 | 2.14E-66 | -1.69E-46 |
| MS.gene052011 | MS.gene41217 | -0.815894 | 7.32E-52 | -1.69E-46 |
| MS.gene052077 | MS.gene41217 | 0.820509 | 6.60E-53 | -1.69E-46 |
| MS.gene052176 | MS.gene41217 | 0.80345 | 3.47E-49 | -1.69E-46 |
| MS.gene052309 | MS.gene41217 | -0.813111 | 3.02E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene41217 | 0.815921 | 7.21E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene41217 | 0.804347 | 2.26E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene41217 | 0.83638 | 9.68E-57 | -1.69E-46 |
| MS.gene05556 | MS.gene41217 | 0.804517 | 2.08E-49 | -1.69E-46 |
| MS.gene056723 | MS.gene41217 | 0.802355 | 5.84E-49 | -1.69E-46 |
| MS.gene056979 | MS.gene41217 | 0.858985 | 5.66E-63 | -1.69E-46 |
| MS.gene05811 | MS.gene41217 | 0.812612 | 3.88E-51 | -1.69E-46 |
| MS.gene058142 | MS.gene41217 | 0.80907 | 2.27E-50 | -1.69E-46 |
| MS.gene059247 | MS.gene41217 | 0.804103 | 2.54E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene41217 | 0.831649 | 1.48E-55 | -1.69E-46 |
| MS.gene060083 | MS.gene41217 | 0.810925 | 9.04E-51 | -1.69E-46 |
| MS.gene060986 | MS.gene41217 | 0.805185 | 1.51E-49 | -1.69E-46 |
| MS.gene061114 | MS.gene41217 | -0.803625 | 3.19E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41217.t1 | MTR_1g049180 | 93.939 | 231 | 7 | 2 | 1 | 231 | 153 | 376 | 4.55e-155 | 437 |
| MS.gene41217.t1 | MTR_5g043180 | 65.888 | 214 | 71 | 2 | 2 | 214 | 160 | 372 | 1.22e-95 | 286 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41217.t1 | AT5G13610 | 68.750 | 256 | 63 | 3 | 1 | 246 | 152 | 400 | 5.67e-118 | 343 |
| MS.gene41217.t1 | AT1G69380 | 61.682 | 214 | 81 | 1 | 2 | 214 | 119 | 332 | 3.14e-90 | 271 |
Find 48 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACCATCCTTTCTCATTTC+AGG | 0.181892 | 1.4:-36061101 | None:intergenic |
| CTCCCATATCTGACCATATT+TGG | 0.221053 | 1.4:-36066890 | None:intergenic |
| CCTGGTTCTTCTTTCTTGAA+TGG | 0.268839 | 1.4:+36060960 | MS.gene41217:CDS |
| ACCATATTTGGCATCTTTCC+AGG | 0.288418 | 1.4:-36066878 | None:intergenic |
| CCACTTGTATTCTAGGTTGA+TGG | 0.321901 | 1.4:+36065902 | MS.gene41217:intron |
| AATTATGTAGTTCTCAAGTT+TGG | 0.333870 | 1.4:+36059889 | MS.gene41217:CDS |
| GTTGATGGAATTCGAACTAT+TGG | 0.339072 | 1.4:+36065753 | MS.gene41217:CDS |
| ATGCCAAATATGGTCAGATA+TGG | 0.351572 | 1.4:+36066887 | MS.gene41217:CDS |
| AGTATTGCTCTTGATTACTA+TGG | 0.353838 | 1.4:+36065792 | MS.gene41217:CDS |
| ATGGCTATTTGAAAATTGTT+AGG | 0.362493 | 1.4:+36061057 | MS.gene41217:CDS |
| GTTCTCAAGTTTGGCAATCT+TGG | 0.366939 | 1.4:+36059898 | MS.gene41217:CDS |
| CGAACTATTGGTAGTGTACT+AGG | 0.382786 | 1.4:+36065765 | MS.gene41217:CDS |
| GTCAGATATGGGAGTATCTC+AGG | 0.391860 | 1.4:+36066899 | MS.gene41217:CDS |
| GCCTGGAAAGATGCCAAATA+TGG | 0.392620 | 1.4:+36066877 | MS.gene41217:CDS |
| TTTAACAGCATTGTTTCTAC+TGG | 0.397693 | 1.4:+36067224 | MS.gene41217:intron |
| TGCATCCATGTGCTCAAAGC+TGG | 0.404184 | 1.4:-36065693 | None:intergenic |
| TTACATAATGTTACAATACT+TGG | 0.404564 | 1.4:+36065728 | MS.gene41217:CDS |
| AATTTGCCTTGCCCACCAAC+TGG | 0.414032 | 1.4:-36066003 | None:intergenic |
| TTACAATACTTGGATGTTGA+TGG | 0.418643 | 1.4:+36065738 | MS.gene41217:CDS |
| TCTTTCTTGAATGGAACAAA+TGG | 0.422190 | 1.4:+36060969 | MS.gene41217:CDS |
| CACATGGATGCAAGGAGGAC+TGG | 0.427102 | 1.4:+36065704 | MS.gene41217:CDS |
| TGATTTCAAGTTGAAATTTG+TGG | 0.429980 | 1.4:+36066957 | MS.gene41217:CDS |
| ATTGTTAGGAAGCATGCATC+TGG | 0.438001 | 1.4:+36061071 | MS.gene41217:CDS |
| GTCCTCGAACATGAGGTTGA+TGG | 0.502037 | 1.4:+36061038 | MS.gene41217:CDS |
| TTTGCTTCCTGAAATGAGAA+AGG | 0.519357 | 1.4:+36061094 | MS.gene41217:CDS |
| TTTACAGATCAGATATCGCC+TGG | 0.521403 | 1.4:+36066860 | MS.gene41217:intron |
| AGCCATCAACCTCATGTTCG+AGG | 0.536713 | 1.4:-36061040 | None:intergenic |
| TGCCAAATATGGTCAGATAT+GGG | 0.539187 | 1.4:+36066888 | MS.gene41217:CDS |
| TTGCTCTTGATTACTATGGC+CGG | 0.547854 | 1.4:+36065796 | MS.gene41217:CDS |
| TGGCAATCTTGGCGATTCCA+AGG | 0.548774 | 1.4:+36059909 | MS.gene41217:CDS |
| CTGGAAAATTTAAGATGCAA+AGG | 0.558742 | 1.4:+36065969 | MS.gene41217:CDS |
| TGTATTCTAGGTTGATGGAA+TGG | 0.567013 | 1.4:+36065907 | MS.gene41217:intron |
| GCTTTGAGCACATGGATGCA+AGG | 0.583404 | 1.4:+36065696 | MS.gene41217:CDS |
| GTTTAATGTCCTCGAACATG+AGG | 0.583906 | 1.4:+36061031 | MS.gene41217:CDS |
| TCTTGATTACTATGGCCGGC+AGG | 0.586842 | 1.4:+36065800 | MS.gene41217:CDS |
| AATCGTGAGATGGAAGCGAC+TGG | 0.588881 | 1.4:+36065950 | MS.gene41217:CDS |
| GCTGATGTGATTCTAAAGCT+CGG | 0.595619 | 1.4:+36066034 | MS.gene41217:CDS |
| TTTCAAGTTGAAATTTGTGG+AGG | 0.601208 | 1.4:+36066960 | MS.gene41217:CDS |
| TACAGACATAAATCGTGAGA+TGG | 0.607409 | 1.4:+36065940 | MS.gene41217:CDS |
| TGGAACAAATGGCTGTTACA+TGG | 0.615782 | 1.4:+36060980 | MS.gene41217:CDS |
| CTTCCTGAAATGAGAAAGGA+TGG | 0.617434 | 1.4:+36061098 | MS.gene41217:CDS |
| TAAAGCTCGGACTATTTGAG+AGG | 0.622265 | 1.4:+36066047 | MS.gene41217:CDS |
| CCATTCAAGAAAGAAGAACC+AGG | 0.624252 | 1.4:-36060960 | None:intergenic |
| ATGTCAAATGAACAAATCAA+CGG | 0.630464 | 1.4:+36067279 | MS.gene41217:CDS |
| GAGGACATTAAACAATACAA+TGG | 0.634285 | 1.4:-36061021 | None:intergenic |
| TCAGATATGGGAGTATCTCA+GGG | 0.654645 | 1.4:+36066900 | MS.gene41217:CDS |
| TCAAATGAACAAATCAACGG+AGG | 0.702502 | 1.4:+36067282 | MS.gene41217:CDS |
| TTGAGCACATGGATGCAAGG+AGG | 0.721239 | 1.4:+36065699 | MS.gene41217:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 36059883 | 36067348 | 36059883 | ID=MS.gene41217 |
| chr1.4 | mRNA | 36059883 | 36067348 | 36059883 | ID=MS.gene41217.t1;Parent=MS.gene41217 |
| chr1.4 | exon | 36059883 | 36059930 | 36059883 | ID=MS.gene41217.t1.exon1;Parent=MS.gene41217.t1 |
| chr1.4 | CDS | 36059883 | 36059930 | 36059883 | ID=cds.MS.gene41217.t1;Parent=MS.gene41217.t1 |
| chr1.4 | exon | 36060957 | 36061119 | 36060957 | ID=MS.gene41217.t1.exon2;Parent=MS.gene41217.t1 |
| chr1.4 | CDS | 36060957 | 36061119 | 36060957 | ID=cds.MS.gene41217.t1;Parent=MS.gene41217.t1 |
| chr1.4 | exon | 36065673 | 36065821 | 36065673 | ID=MS.gene41217.t1.exon3;Parent=MS.gene41217.t1 |
| chr1.4 | CDS | 36065673 | 36065821 | 36065673 | ID=cds.MS.gene41217.t1;Parent=MS.gene41217.t1 |
| chr1.4 | exon | 36065917 | 36066068 | 36065917 | ID=MS.gene41217.t1.exon4;Parent=MS.gene41217.t1 |
| chr1.4 | CDS | 36065917 | 36066068 | 36065917 | ID=cds.MS.gene41217.t1;Parent=MS.gene41217.t1 |
| chr1.4 | exon | 36066867 | 36066981 | 36066867 | ID=MS.gene41217.t1.exon5;Parent=MS.gene41217.t1 |
| chr1.4 | CDS | 36066867 | 36066981 | 36066867 | ID=cds.MS.gene41217.t1;Parent=MS.gene41217.t1 |
| chr1.4 | exon | 36067232 | 36067348 | 36067232 | ID=MS.gene41217.t1.exon6;Parent=MS.gene41217.t1 |
| chr1.4 | CDS | 36067232 | 36067348 | 36067232 | ID=cds.MS.gene41217.t1;Parent=MS.gene41217.t1 |
| Gene Sequence |
| Protein sequence |
