AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene41892


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene41892.t1 MTR_7g088520 92.199 141 11 0 1 141 1 141 1.70e-88 254
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGGGTTGTGGAGGGAAT+TGG 0.246105 7.2:-20250281 None:intergenic
TGTGGAGGGAATTGGGAAAA+TGG 0.293091 7.2:-20250273 None:intergenic
TTGGGGTTGTGGAGGGAATT+GGG 0.318138 7.2:-20250280 None:intergenic
ACTCTTCAATTTCTTCATCT+TGG 0.341918 7.2:-20250734 None:intergenic
TTCTGTAGATAAGCTTCCTT+TGG 0.349663 7.2:+20250588 MS.gene41892:CDS
AGTTGAGAAAGAAGGGTAAT+TGG 0.374960 7.2:+20250704 MS.gene41892:CDS
TTCAGACCCATCTGCAACAT+TGG 0.400037 7.2:-20250536 None:intergenic
AAGAGGAAGTTGAGAAAGAA+GGG 0.409426 7.2:+20250697 MS.gene41892:CDS
TTCAATTTCTTCATCTTGGA+AGG 0.432102 7.2:-20250730 None:intergenic
TCCTTTGGAATCAAAATTGA+AGG 0.438799 7.2:+20250603 MS.gene41892:CDS
AAAGAGGAAGTTGAGAAAGA+AGG 0.445671 7.2:+20250696 MS.gene41892:CDS
AAGTCCCTTCAATGAGTTTG+TGG 0.462466 7.2:-20250443 None:intergenic
AAATGGAGGAAGAAGAAAGA+GGG 0.478465 7.2:-20250256 None:intergenic
GAAAGAGACTTGTTCAAAAG+AGG 0.482878 7.2:+20250680 MS.gene41892:CDS
TTGATAAGAAGATAAGGTTG+AGG 0.486048 7.2:+20250656 MS.gene41892:CDS
TGGAATCAAAATTGAAGGAG+AGG 0.491503 7.2:+20250608 MS.gene41892:CDS
TGAAGCTTGATAAGAAGATA+AGG 0.493499 7.2:+20250650 MS.gene41892:CDS
TCCCTCGGTTTCACAACAAA+GGG 0.494277 7.2:-20250478 None:intergenic
AGAAAGAGGGGTGAGTGTGA+TGG 0.496412 7.2:-20250243 None:intergenic
GGAATCAAAATTGAAGGAGA+GGG 0.508757 7.2:+20250609 MS.gene41892:CDS
AGTCCCTTCAATGAGTTTGT+GGG 0.524517 7.2:-20250442 None:intergenic
TTTGCCCACAAACTCATTGA+AGG 0.534777 7.2:+20250438 MS.gene41892:CDS
TTCCCTCGGTTTCACAACAA+AGG 0.535738 7.2:-20250479 None:intergenic
ACTGAATCCAATGTTGCAGA+TGG 0.538749 7.2:+20250529 MS.gene41892:CDS
ACGGTACTTACTTGGGGTTG+TGG 0.540099 7.2:-20250291 None:intergenic
TACTTACTTGGGGTTGTGGA+GGG 0.543624 7.2:-20250287 None:intergenic
TGAAGGAGAGGGAAGAGCAG+AGG 0.551586 7.2:+20250620 MS.gene41892:CDS
ATTGAAGGGACTTCGAATCT+CGG 0.553791 7.2:+20250453 MS.gene41892:CDS
GTACTTACTTGGGGTTGTGG+AGG 0.554680 7.2:-20250288 None:intergenic
TTGCCCACAAACTCATTGAA+GGG 0.555855 7.2:+20250439 MS.gene41892:CDS
AATTTCTTCATCTTGGAAGG+TGG 0.562678 7.2:-20250727 None:intergenic
AAAATGGAGGAAGAAGAAAG+AGG 0.565844 7.2:-20250257 None:intergenic
AATGGAGGAAGAAGAAAGAG+GGG 0.583049 7.2:-20250255 None:intergenic
CACCCTTTGTTGTGAAACCG+AGG 0.599124 7.2:+20250476 MS.gene41892:CDS
GGAGGGAATTGGGAAAATGG+AGG 0.607476 7.2:-20250270 None:intergenic
CTGAATCCAATGTTGCAGAT+GGG 0.613616 7.2:+20250530 MS.gene41892:CDS
ACCCTTTGTTGTGAAACCGA+GGG 0.617169 7.2:+20250477 MS.gene41892:CDS
TCAGTTTCAAGAGTGCACGT+TGG 0.725975 7.2:+20250415 MS.gene41892:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAGAATAATAAAAACAT+GGG - chr7.2:20250351-20250370 None:intergenic 15.0%
!! TAACAAGAATAATAAAAACA+TGG - chr7.2:20250352-20250371 None:intergenic 15.0%
! AATGTATTGTGGAGAAATTA+AGG - chr7.2:20250328-20250347 None:intergenic 25.0%
!! ATAAAAATTGCATTTTCCCT+CGG - chr7.2:20250496-20250515 None:intergenic 25.0%
AAAAACATGGGAATGTATTG+TGG - chr7.2:20250339-20250358 None:intergenic 30.0%
AATTAAGGAAGAAGAAGTGA+CGG - chr7.2:20250313-20250332 None:intergenic 30.0%
ACTCTTCAATTTCTTCATCT+TGG - chr7.2:20250737-20250756 None:intergenic 30.0%
TGAAGCTTGATAAGAAGATA+AGG + chr7.2:20250650-20250669 MS.gene41892:CDS 30.0%
TTCAATTTCTTCATCTTGGA+AGG - chr7.2:20250733-20250752 None:intergenic 30.0%
TTGATAAGAAGATAAGGTTG+AGG + chr7.2:20250656-20250675 MS.gene41892:CDS 30.0%
!! TCCTTTGGAATCAAAATTGA+AGG + chr7.2:20250603-20250622 MS.gene41892:CDS 30.0%
!!! TCCTTCAATTTTGATTCCAA+AGG - chr7.2:20250607-20250626 None:intergenic 30.0%
AAAATGGAGGAAGAAGAAAG+AGG - chr7.2:20250260-20250279 None:intergenic 35.0%
AAAGAGGAAGTTGAGAAAGA+AGG + chr7.2:20250696-20250715 MS.gene41892:CDS 35.0%
AAATGGAGGAAGAAGAAAGA+GGG - chr7.2:20250259-20250278 None:intergenic 35.0%
AAGAGGAAGTTGAGAAAGAA+GGG + chr7.2:20250697-20250716 MS.gene41892:CDS 35.0%
AATTTCTTCATCTTGGAAGG+TGG - chr7.2:20250730-20250749 None:intergenic 35.0%
AGTTGAGAAAGAAGGGTAAT+TGG + chr7.2:20250704-20250723 MS.gene41892:CDS 35.0%
GGAATCAAAATTGAAGGAGA+GGG + chr7.2:20250609-20250628 MS.gene41892:CDS 35.0%
TGGAATCAAAATTGAAGGAG+AGG + chr7.2:20250608-20250627 MS.gene41892:CDS 35.0%
TTCTGTAGATAAGCTTCCTT+TGG + chr7.2:20250588-20250607 MS.gene41892:CDS 35.0%
! GAAAGAGACTTGTTCAAAAG+AGG + chr7.2:20250680-20250699 MS.gene41892:CDS 35.0%
AAGAAGTGACGGTACTTACT+TGG - chr7.2:20250302-20250321 None:intergenic 40.0%
AAGTCCCTTCAATGAGTTTG+TGG - chr7.2:20250446-20250465 None:intergenic 40.0%
AATGGAGGAAGAAGAAAGAG+GGG - chr7.2:20250258-20250277 None:intergenic 40.0%
ACTGAATCCAATGTTGCAGA+TGG + chr7.2:20250529-20250548 MS.gene41892:CDS 40.0%
AGAAGTGACGGTACTTACTT+GGG - chr7.2:20250301-20250320 None:intergenic 40.0%
AGTCCCTTCAATGAGTTTGT+GGG - chr7.2:20250445-20250464 None:intergenic 40.0%
ATTGAAGGGACTTCGAATCT+CGG + chr7.2:20250453-20250472 MS.gene41892:CDS 40.0%
CTGAATCCAATGTTGCAGAT+GGG + chr7.2:20250530-20250549 MS.gene41892:CDS 40.0%
TTGCCCACAAACTCATTGAA+GGG + chr7.2:20250439-20250458 MS.gene41892:CDS 40.0%
TTTGCCCACAAACTCATTGA+AGG + chr7.2:20250438-20250457 MS.gene41892:CDS 40.0%
GAAGTGACGGTACTTACTTG+GGG - chr7.2:20250300-20250319 None:intergenic 45.0%
TACTTACTTGGGGTTGTGGA+GGG - chr7.2:20250290-20250309 None:intergenic 45.0%
TCCCTCGGTTTCACAACAAA+GGG - chr7.2:20250481-20250500 None:intergenic 45.0%
TGTGGAGGGAATTGGGAAAA+TGG - chr7.2:20250276-20250295 None:intergenic 45.0%
TTCAGACCCATCTGCAACAT+TGG - chr7.2:20250539-20250558 None:intergenic 45.0%
TTCCCTCGGTTTCACAACAA+AGG - chr7.2:20250482-20250501 None:intergenic 45.0%
! ACCCTTTGTTGTGAAACCGA+GGG + chr7.2:20250477-20250496 MS.gene41892:CDS 45.0%
! TCAGTTTCAAGAGTGCACGT+TGG + chr7.2:20250415-20250434 MS.gene41892:intron 45.0%
ACGGTACTTACTTGGGGTTG+TGG - chr7.2:20250294-20250313 None:intergenic 50.0%
AGAAAGAGGGGTGAGTGTGA+TGG - chr7.2:20250246-20250265 None:intergenic 50.0%
GGAGGGAATTGGGAAAATGG+AGG - chr7.2:20250273-20250292 None:intergenic 50.0%
GTACTTACTTGGGGTTGTGG+AGG - chr7.2:20250291-20250310 None:intergenic 50.0%
TTGGGGTTGTGGAGGGAATT+GGG - chr7.2:20250283-20250302 None:intergenic 50.0%
! CACCCTTTGTTGTGAAACCG+AGG + chr7.2:20250476-20250495 MS.gene41892:CDS 50.0%
CTTGGGGTTGTGGAGGGAAT+TGG - chr7.2:20250284-20250303 None:intergenic 55.0%
TGAAGGAGAGGGAAGAGCAG+AGG + chr7.2:20250620-20250639 MS.gene41892:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 20250221 20250762 20250221 ID=MS.gene41892
chr7.2 mRNA 20250221 20250762 20250221 ID=MS.gene41892.t1;Parent=MS.gene41892
chr7.2 exon 20250221 20250302 20250221 ID=MS.gene41892.t1.exon1;Parent=MS.gene41892.t1
chr7.2 CDS 20250221 20250302 20250221 ID=cds.MS.gene41892.t1;Parent=MS.gene41892.t1
chr7.2 exon 20250419 20250762 20250419 ID=MS.gene41892.t1.exon2;Parent=MS.gene41892.t1
chr7.2 CDS 20250419 20250762 20250419 ID=cds.MS.gene41892.t1;Parent=MS.gene41892.t1
Gene Sequence

>MS.gene41892

ATGGCACTTCTCTGCTTCAATTCCATCACACTCACCCCTCTTTCTTCTTCCTCCATTTTCCCAATTCCCTCCACAACCCCAAGTAAGTACCGTCACTTCTTCTTCCTTAATTTCTCCACAATACATTCCCATGTTTTTATTATTCTTGTTATTGTTTCTTTTATCTTGATTCTCTCGAACTATGTTTTTGTTTATCAGTTTCAAGAGTGCACGTTGGTTTGCCCACAAACTCATTGAAGGGACTTCGAATCTCGGCACCCTTTGTTGTGAAACCGAGGGAAAATGCAATTTTTATTGCATCTGCTACTACTGAATCCAATGTTGCAGATGGGTCTGAAAGTGAAAGCAAGAAAGAGAGTGATGCTGTTTCTGTAGATAAGCTTCCTTTGGAATCAAAATTGAAGGAGAGGGAAGAGCAGAGGTTGAAGATGAAGCTTGATAAGAAGATAAGGTTGAGGAGAAAGAGACTTGTTCAAAAGAGGAAGTTGAGAAAGAAGGGTAATTGGCCACCTTCCAAGATGAAGAAATTGAAGAGTGTTTGA

Protein sequence

>MS.gene41892.t1

MALLCFNSITLTPLSSSSIFPIPSTTPISRVHVGLPTNSLKGLRISAPFVVKPRENAIFIASATTESNVADGSESESKKESDAVSVDKLPLESKLKEREEQRLKMKLDKKIRLRRKRLVQKRKLRKKGNWPPSKMKKLKSV