AlfalfaGEDB | MS.gene44270

M. sativa cultivar XinJiangDaYe / MS.gene44270

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049404	MS.gene44270	0.804676	1.93E-49	-1.69E-46
MS.gene049850	MS.gene44270	0.83324	5.97E-56	-1.69E-46
MS.gene049873	MS.gene44270	0.806371	8.50E-50	-1.69E-46
MS.gene050020	MS.gene44270	0.801936	7.12E-49	-1.69E-46
MS.gene050156	MS.gene44270	0.807051	6.11E-50	-1.69E-46
MS.gene05157	MS.gene44270	0.802285	6.03E-49	-1.69E-46
MS.gene051594	MS.gene44270	0.812296	4.55E-51	-1.69E-46
MS.gene051719	MS.gene44270	0.802435	5.62E-49	-1.69E-46
MS.gene051720	MS.gene44270	0.811546	6.63E-51	-1.69E-46
MS.gene051744	MS.gene44270	0.802096	6.60E-49	-1.69E-46
MS.gene051957	MS.gene44270	0.818387	2.01E-52	-1.69E-46
MS.gene052020	MS.gene44270	0.813073	3.08E-51	-1.69E-46
MS.gene052519	MS.gene44270	0.817425	3.32E-52	-1.69E-46
MS.gene052611	MS.gene44270	0.815475	9.07E-52	-1.69E-46
MS.gene052773	MS.gene44270	0.81591	7.26E-52	-1.69E-46
MS.gene052863	MS.gene44270	0.820827	5.58E-53	-1.69E-46
MS.gene053226	MS.gene44270	0.833428	5.36E-56	-1.69E-46
MS.gene053599	MS.gene44270	0.808032	3.78E-50	-1.69E-46
MS.gene053883	MS.gene44270	0.801795	7.61E-49	-1.69E-46
MS.gene053900	MS.gene44270	0.817937	2.54E-52	-1.69E-46
MS.gene053998	MS.gene44270	0.812629	3.85E-51	-1.69E-46
MS.gene05415	MS.gene44270	0.817903	2.59E-52	-1.69E-46
MS.gene054651	MS.gene44270	0.819935	8.94E-53	-1.69E-46
MS.gene055466	MS.gene44270	0.809804	1.58E-50	-1.69E-46
MS.gene055512	MS.gene44270	0.807123	5.90E-50	-1.69E-46
MS.gene055818	MS.gene44270	0.833169	6.22E-56	-1.69E-46
MS.gene056050	MS.gene44270	0.812822	3.49E-51	-1.69E-46
MS.gene056173	MS.gene44270	0.818471	1.93E-52	-1.69E-46
MS.gene056366	MS.gene44270	0.838921	2.16E-57	-1.69E-46
MS.gene056498	MS.gene44270	0.815136	1.08E-51	-1.69E-46
MS.gene057060	MS.gene44270	0.814783	1.29E-51	-1.69E-46
MS.gene057414	MS.gene44270	0.819167	1.34E-52	-1.69E-46
MS.gene057444	MS.gene44270	0.832361	9.88E-56	-1.69E-46
MS.gene058625	MS.gene44270	0.836862	7.29E-57	-1.69E-46
MS.gene058829	MS.gene44270	0.80986	1.54E-50	-1.69E-46
MS.gene058979	MS.gene44270	0.812418	4.28E-51	-1.69E-46
MS.gene059272	MS.gene44270	0.800597	1.34E-48	-1.69E-46
MS.gene060519	MS.gene44270	0.806089	9.74E-50	-1.69E-46
MS.gene060521	MS.gene44270	0.822754	2.00E-53	-1.69E-46
MS.gene060587	MS.gene44270	0.803384	3.58E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 7 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCATGTAACCAATCCAATTC+TGG	0.330330	5.4:-22771523	MS.gene44270:CDS
AGAACATCAAGTTCCAGAAT+TGG	0.346345	5.4:+22771510	None:intergenic
GTTATTGCAGGAAAGTTGAA+TGG	0.435756	5.4:-22771582	MS.gene44270:intron
GTTATTGCAGGAAAGTTGAA+TGG	0.435756	5.4:-22772080	MS.gene44270:intron
ATCAAGTTCCAGAATTGGAT+TGG	0.469272	5.4:+22771515	None:intergenic
ATAAAAGAAGAACATGGGTA+AGG	0.558560	5.4:-22772633	None:intergenic
GAAGATGATGAATATCACCA+TGG	0.681493	5.4:-22772354	MS.gene44270:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATAATGAAGATTAGAAAA+AGG	+	chr5.4:22771974-22771993	None:intergenic	15.0%
!!	AAGAATCATTGAATATAAAA+GGG	-	chr5.4:22771855-22771874	MS.gene44270:intron	15.0%
!!	AATTGTTTCAAAAACAAAAA+AGG	-	chr5.4:22772404-22772423	MS.gene44270:intron	15.0%
!!	AATTTATCAGAAATTATTTG+TGG	-	chr5.4:22771912-22771931	MS.gene44270:intron	15.0%
!!	TTAATCTACTTATTATAGTT+TGG	+	chr5.4:22771627-22771646	None:intergenic	15.0%
!!	TTATTTGTATTTATCTTCAA+CGG	-	chr5.4:22771988-22772007	MS.gene44270:intron	15.0%
!!!	TTTAAGTATTAATTTTTTGC+TGG	-	chr5.4:22772165-22772184	MS.gene44270:intron	15.0%
!!	AAAACAAAAAAGGAGTTTAA+TGG	-	chr5.4:22772414-22772433	MS.gene44270:intron	20.0%
!!	AGAATCATTGAATATAAAAG+GGG	-	chr5.4:22771856-22771875	MS.gene44270:intron	20.0%
!!	AGTTTAATATGATGATATGA+AGG	+	chr5.4:22771548-22771567	None:intergenic	20.0%
!!	GAAGAATCATTGAATATAAA+AGG	-	chr5.4:22771854-22771873	MS.gene44270:intron	20.0%
!!	TTTCTGATAAATTCATAAGT+TGG	+	chr5.4:22771905-22771924	None:intergenic	20.0%
!!	TTTGTAGATCAAAAAAGATA+AGG	+	chr5.4:22772508-22772527	None:intergenic	20.0%
!!!	AAATGAAGACAATTTTACAA+AGG	+	chr5.4:22771516-22771535	None:intergenic	20.0%
!	TAATGAAGATTAGAAAAAGG+AGG	+	chr5.4:22771971-22771990	None:intergenic	25.0%
!	TTGAAAATATGATATGTTGC+AGG	-	chr5.4:22772231-22772250	MS.gene44270:intron	25.0%
!!	ACAATATTTCCTTTGTTTGT+TGG	-	chr5.4:22771713-22771732	MS.gene44270:intron	25.0%
!!!	ATGTGTCATTTATTTTGTTG+AGG	+	chr5.4:22772146-22772165	None:intergenic	25.0%
!!!	TCTTGTCTGTTATGTTTTTA+TGG	-	chr5.4:22771832-22771851	MS.gene44270:intron	25.0%
	AAAGACATCCTTAGAAACAA+AGG	-	chr5.4:22772295-22772314	MS.gene44270:intron	30.0%
	AAAGGGGAAAACAATAAAGA+TGG	-	chr5.4:22771872-22771891	MS.gene44270:intron	30.0%
	AAGACATCCTTAGAAACAAA+GGG	-	chr5.4:22772296-22772315	MS.gene44270:intron	30.0%
	ACAAAAAAGGAGTTTAATGG+TGG	-	chr5.4:22772417-22772436	MS.gene44270:intron	30.0%
	TGATCTACAAATGTTATTGC+AGG	-	chr5.4:22772019-22772038	MS.gene44270:intron	30.0%
	TTTATCTGTCCAACAAACAA+AGG	+	chr5.4:22771725-22771744	None:intergenic	30.0%
!	GTTTGGATGTGAATGATTTT+TGG	+	chr5.4:22771610-22771629	None:intergenic	30.0%
	AAGATTAGAAAAAGGAGGAG+AGG	+	chr5.4:22771966-22771985	None:intergenic	35.0%
	AGAACATCAAGTTCCAGAAT+TGG	+	chr5.4:22772604-22772623	None:intergenic	35.0%
	ATCAAGTTCCAGAATTGGAT+TGG	+	chr5.4:22772599-22772618	None:intergenic	35.0%
	CAAAAAAGATAAGGATGCTG+AGG	+	chr5.4:22772499-22772518	None:intergenic	35.0%
	GAAGATGATGAATATCACCA+TGG	-	chr5.4:22771757-22771776	MS.gene44270:intron	35.0%
	GTTATTGCAGGAAAGTTGAA+TGG	-	chr5.4:22772031-22772050	MS.gene44270:intron	35.0%
	TCATGTAACCAATCCAATTC+TGG	-	chr5.4:22772588-22772607	MS.gene44270:intron	35.0%
!	TTTTCCTTCTTACACTTCCA+TGG	+	chr5.4:22771777-22771796	None:intergenic	35.0%
!	TTTTCTTCAGCTGCATAACA+TGG	-	chr5.4:22771578-22771597	MS.gene44270:intron	35.0%
!!	AACTCTTCCCTTTGTTTCTA+AGG	+	chr5.4:22772306-22772325	None:intergenic	35.0%
!	ATCACCATGGAAGTGTAAGA+AGG	-	chr5.4:22771770-22771789	MS.gene44270:intron	40.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	22771491	22772642	22771491	ID=MS.gene44270
chr5.4	mRNA	22771491	22772642	22771491	ID=MS.gene44270.t1;Parent=MS.gene44270
chr5.4	exon	22772634	22772642	22772634	ID=MS.gene44270.t1.exon1;Parent=MS.gene44270.t1
chr5.4	CDS	22772634	22772642	22772634	ID=cds.MS.gene44270.t1;Parent=MS.gene44270.t1
chr5.4	exon	22772350	22772393	22772350	ID=MS.gene44270.t1.exon2;Parent=MS.gene44270.t1
chr5.4	CDS	22772350	22772393	22772350	ID=cds.MS.gene44270.t1;Parent=MS.gene44270.t1
chr5.4	exon	22772034	22772092	22772034	ID=MS.gene44270.t1.exon3;Parent=MS.gene44270.t1
chr5.4	CDS	22772034	22772092	22772034	ID=cds.MS.gene44270.t1;Parent=MS.gene44270.t1
chr5.4	exon	22771491	22771594	22771491	ID=MS.gene44270.t1.exon4;Parent=MS.gene44270.t1
chr5.4	CDS	22771491	22771594	22771491	ID=cds.MS.gene44270.t1;Parent=MS.gene44270.t1

Gene Sequence

>MS.gene44270

ATGGGTAAGGTGAACTCTTTTTCCTTTGTAAAATTGTCTTCATTTTTTTTATGTCCTTCATATCATCATATTAAACTAAGTTTACTTTTTTCTTCAGCTGCATAACATGGCAAATACCAAAAATCATTCACATCCAAACTATAATAAGTAGATTAATACAAATCTATAATAAAGTTTTACACTATACTCAATCTTCTTCAAATATATTGATGTTCTTGCACAACAATATTTCCTTTGTTTGTTGGACAGATAAATAGACAAGAAATGAAGATGATGAATATCACCATGGAAGTGTAAGAAGGAAAAAAGCACGAGAAAATAAAGATCAAAAGAAGTAGCAATCTTGTCTGTTATGTTTTTATGGAAGAATCATTGAATATAAAAGGGGAAAACAATAAAGATGGAGAAGAACCAACTTATGAATTTATCAGAAATTATTTGTGGAGAAGAACTTTTCATTATCTAACATGTACCTCTCCTCCTTTTTCTAATCTTCATTATTTGTATTTATCTTCAACGGCAATTTTTTGATCTACAAATGTTATTGCAGGAAAGTTGAATGGAGTTCTTGTCTCATCAGCAAACTACAAACAAACAAAGAAAACATCAGTAAGTAAATGATGAGAATTACTAATAAGTTTAGTACATAAATCCTCAACAAAATAAATGACACATTTAAGTATTAATTTTTTGCTGGAAATGATGAATTAGTGTCTTGTATTTGTATGTTGCTAATAAAATTGAAAATATGATATGTTGCAGGAAAGTCAATTGATGTTCTTGTCACTTCAACAAACTACAAAGAAAGACATCCTTAGAAACAAAGGGAAGAGTTTGATTAGTATATGTTTTCTTATGTGATTATATACTGTTATGAAGAAATAAAGAAAATAACTACATAAGAAACGAGTTTAATTGTTTCAAAAACAAAAAAGGAGTTTAATGGTGGTGTGTATAAAGATACAAGTGAGATTATCTAAAGTTATTTTATTTTATAAATAAATACCTCAGCATCCTTATCTTTTTTGATCTACAAATGTTATTGCAGGAAAGTTGAATGGAGTTCTTGTCTCATCAGCAAACTACAAACAAAGAAATCATGTAACCAATCCAATTCTGGAACTTGATGTTCTTAAGCTAGAAATCTCATAG

Protein sequence

>MS.gene44270.t1

MGKINRQEMKMMNITMEVKVEWSSCLISKLQTNKENIRKLNGVLVSSANYKQRNHVTNPILELDVLKLEIS