Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50323.t1 | XP_003613603.2 | 90.3 | 93 | 8 | 1 | 1 | 92 | 118 | 210 | 9.50E-06 | 59.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50323.t1 | G7KB20 | 90.3 | 93 | 8 | 1 | 1 | 92 | 118 | 210 | 6.9e-06 | 59.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene50323 | 0.823463 | 1.37E-53 | -1.69E-46 |
| MS.gene051594 | MS.gene50323 | 0.800848 | 1.19E-48 | -1.69E-46 |
| MS.gene052037 | MS.gene50323 | 0.813118 | 3.01E-51 | -1.69E-46 |
| MS.gene052447 | MS.gene50323 | 0.857335 | 1.75E-62 | -1.69E-46 |
| MS.gene05329 | MS.gene50323 | 0.812217 | 4.74E-51 | -1.69E-46 |
| MS.gene05415 | MS.gene50323 | 0.840179 | 1.02E-57 | -1.69E-46 |
| MS.gene05423 | MS.gene50323 | 0.803996 | 2.67E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene50323 | 0.816877 | 4.41E-52 | -1.69E-46 |
| MS.gene055228 | MS.gene50323 | 0.814029 | 1.90E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene50323 | 0.806155 | 9.44E-50 | -1.69E-46 |
| MS.gene055952 | MS.gene50323 | 0.81019 | 1.30E-50 | -1.69E-46 |
| MS.gene056174 | MS.gene50323 | 0.808419 | 3.13E-50 | -1.69E-46 |
| MS.gene056272 | MS.gene50323 | 0.812117 | 4.98E-51 | -1.69E-46 |
| MS.gene056539 | MS.gene50323 | 0.807246 | 5.55E-50 | -1.69E-46 |
| MS.gene059584 | MS.gene50323 | 0.815371 | 9.57E-52 | -1.69E-46 |
| MS.gene060134 | MS.gene50323 | 0.802166 | 6.38E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene50323 | -0.801429 | 9.04E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50323.t1 | MTR_5g038570 | 91.398 | 93 | 7 | 1 | 1 | 92 | 118 | 210 | 3.81e-32 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGTCCTCACCAAGGTTATT+TGG | 0.226691 | 4.4:+78125214 | None:intergenic |
| GTGGGAACTGTCGGTGCTTT+GGG | 0.318992 | 4.4:-78125361 | MS.gene50323:CDS |
| TGTGGGAACTGTCGGTGCTT+TGG | 0.357110 | 4.4:-78125362 | MS.gene50323:CDS |
| ACAGGTCCTGCTGCAATTGC+GGG | 0.391461 | 4.4:-78125319 | MS.gene50323:CDS |
| GCTTACCGTTGTTGACTTGA+AGG | 0.402879 | 4.4:+78125168 | None:intergenic |
| GCTTTGGGAGCAATAGCATT+AGG | 0.416841 | 4.4:-78125346 | MS.gene50323:CDS |
| CTTAAATGTCTCATTGCTCC+TGG | 0.451122 | 4.4:-78124898 | MS.gene50323:CDS |
| TAACATTACATGGACATTCC+AGG | 0.464905 | 4.4:+78124880 | None:intergenic |
| GCTGCAGTTGCTCTATCAGC+TGG | 0.482459 | 4.4:-78125409 | MS.gene50323:CDS |
| GCAATAGCATTAGGAGCTAC+AGG | 0.486153 | 4.4:-78125337 | MS.gene50323:CDS |
| GCTGGTACAACAACAGCTGC+TGG | 0.486387 | 4.4:-78125391 | MS.gene50323:CDS |
| TACAGGTCCTGCTGCAATTG+CGG | 0.492454 | 4.4:-78125320 | MS.gene50323:CDS |
| GGACTAACTGCTGCTGCAGT+AGG | 0.505130 | 4.4:-78125298 | MS.gene50323:CDS |
| ATCCATAATATAACATTACA+TGG | 0.521394 | 4.4:+78124870 | None:intergenic |
| CTTACCGTTGTTGACTTGAA+GGG | 0.522281 | 4.4:+78125169 | None:intergenic |
| CCAAGGTTATTTGGAGCTTG+CGG | 0.529727 | 4.4:+78125223 | None:intergenic |
| GTTAGTCCCGCAATTGCAGC+AGG | 0.531116 | 4.4:+78125313 | None:intergenic |
| GGAGTTGGTGTGGGAACTGT+CGG | 0.534103 | 4.4:-78125370 | MS.gene50323:CDS |
| TTGCGGAGCATCTTTACCCA+TGG | 0.540938 | 4.4:+78125240 | None:intergenic |
| GGAGCAATGAGACATTTAAG+AGG | 0.566018 | 4.4:+78124901 | None:intergenic |
| CCGCAAGCTCCAAATAACCT+TGG | 0.576255 | 4.4:-78125223 | MS.gene50323:CDS |
| AACAGCTGCTGGAGTTGGTG+TGG | 0.592073 | 4.4:-78125380 | MS.gene50323:CDS |
| TGCTGCAGTAGGTGCAGTAG+TGG | 0.607069 | 4.4:-78125287 | MS.gene50323:CDS |
| CTGATAGAGCAACTGCAGCA+AGG | 0.620031 | 4.4:+78125413 | None:intergenic |
| ACAACAACAGCTGCTGGAGT+TGG | 0.624041 | 4.4:-78125385 | MS.gene50323:CDS |
| GCAGTAGGTGCAGTAGTGGT+AGG | 0.640998 | 4.4:-78125283 | MS.gene50323:CDS |
| TTGCTTTGTAGTCCTCACCA+AGG | 0.660537 | 4.4:+78125206 | None:intergenic |
| AGCTCCAAATAACCTTGGTG+AGG | 0.665982 | 4.4:-78125218 | MS.gene50323:CDS |
| ACAGCTGCTGGAGTTGGTGT+GGG | 0.669071 | 4.4:-78125379 | MS.gene50323:CDS |
| GTAGGTGCAGTAGTGGTAGG+TGG | 0.682399 | 4.4:-78125280 | MS.gene50323:CDS |
| TGTTGCTGCAGATAAAACCA+TGG | 0.706316 | 4.4:-78125257 | MS.gene50323:CDS |
| GTTGCTGCAGATAAAACCAT+GGG | 0.711616 | 4.4:-78125256 | MS.gene50323:CDS |
| AGAACCCTTCAAGTCAACAA+CGG | 0.731950 | 4.4:-78125173 | MS.gene50323:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTTGAGCTTATTACACTTT+TGG | - | chr4.4:78125208-78125227 | MS.gene50323:CDS | 25.0% |
| ! | GTCACAAGGATTTTAAATTG+TGG | + | chr4.4:78125375-78125394 | None:intergenic | 30.0% |
| ! | TTTTGTAATGCGGTAAAATG+CGG | + | chr4.4:78125333-78125352 | None:intergenic | 30.0% |
| !!! | GTTTAAGCTTTTTTCTTCCT+TGG | - | chr4.4:78125230-78125249 | MS.gene50323:CDS | 30.0% |
| AAAATATCTCTCCCTAAGAG+AGG | - | chr4.4:78125273-78125292 | MS.gene50323:CDS | 35.0% | |
| TCAAAGATTTCTCTCAACCA+AGG | + | chr4.4:78125250-78125269 | None:intergenic | 35.0% | |
| ! | CACAGTGAACTTTTGTAATG+CGG | + | chr4.4:78125343-78125362 | None:intergenic | 35.0% |
| ACAAATGGTCTCCTCTCTTA+GGG | + | chr4.4:78125287-78125306 | None:intergenic | 40.0% | |
| AGAACCCTTCAAGTCAACAA+CGG | - | chr4.4:78125139-78125158 | MS.gene50323:intron | 40.0% | |
| CTTAAATGTCTCATTGCTCC+TGG | - | chr4.4:78125414-78125433 | MS.gene50323:CDS | 40.0% | |
| GGAGCAATGAGACATTTAAG+AGG | + | chr4.4:78125414-78125433 | None:intergenic | 40.0% | |
| GTTGCTGCAGATAAAACCAT+GGG | - | chr4.4:78125056-78125075 | MS.gene50323:intron | 40.0% | |
| TACAAATGGTCTCCTCTCTT+AGG | + | chr4.4:78125288-78125307 | None:intergenic | 40.0% | |
| TAGTCCTCACCAAGGTTATT+TGG | + | chr4.4:78125101-78125120 | None:intergenic | 40.0% | |
| TCTTCTTGAGTTCAGTCACA+AGG | + | chr4.4:78125389-78125408 | None:intergenic | 40.0% | |
| TGTTGCTGCAGATAAAACCA+TGG | - | chr4.4:78125055-78125074 | MS.gene50323:intron | 40.0% | |
| !! | CTTACCGTTGTTGACTTGAA+GGG | + | chr4.4:78125146-78125165 | None:intergenic | 40.0% |
| AGACCATTTGTAGCTGAGAC+AGG | - | chr4.4:78125296-78125315 | MS.gene50323:CDS | 45.0% | |
| AGCTCCAAATAACCTTGGTG+AGG | - | chr4.4:78125094-78125113 | MS.gene50323:intron | 45.0% | |
| CAACAACGGTAAGCAAACGA+TGG | - | chr4.4:78125153-78125172 | MS.gene50323:intron | 45.0% | |
| GCAATAGCATTAGGAGCTAC+AGG | - | chr4.4:78124975-78124994 | MS.gene50323:intron | 45.0% | |
| TTGCTTTGTAGTCCTCACCA+AGG | + | chr4.4:78125109-78125128 | None:intergenic | 45.0% | |
| ! | CCAAGGTTATTTGGAGCTTG+CGG | + | chr4.4:78125092-78125111 | None:intergenic | 45.0% |
| !! | GCTTACCGTTGTTGACTTGA+AGG | + | chr4.4:78125147-78125166 | None:intergenic | 45.0% |
| !! | GCTTTGGGAGCAATAGCATT+AGG | - | chr4.4:78124966-78124985 | MS.gene50323:intron | 45.0% |
| ACAACAACAGCTGCTGGAGT+TGG | - | chr4.4:78124927-78124946 | MS.gene50323:intron | 50.0% | |
| CCGCAAGCTCCAAATAACCT+TGG | - | chr4.4:78125089-78125108 | MS.gene50323:intron | 50.0% | |
| CTGATAGAGCAACTGCAGCA+AGG | + | chr4.4:78124902-78124921 | None:intergenic | 50.0% | |
| TACAGGTCCTGCTGCAATTG+CGG | - | chr4.4:78124992-78125011 | MS.gene50323:intron | 50.0% | |
| ! | TTGCGGAGCATCTTTACCCA+TGG | + | chr4.4:78125075-78125094 | None:intergenic | 50.0% |
| ACAGGTCCTGCTGCAATTGC+GGG | - | chr4.4:78124993-78125012 | MS.gene50323:intron | 55.0% | |
| GCAGTAGGTGCAGTAGTGGT+AGG | - | chr4.4:78125029-78125048 | MS.gene50323:intron | 55.0% | |
| GCGCCTGTCTCAGCTACAAA+TGG | + | chr4.4:78125302-78125321 | None:intergenic | 55.0% | |
| GCTGCAGTTGCTCTATCAGC+TGG | - | chr4.4:78124903-78124922 | MS.gene50323:CDS | 55.0% | |
| GCTGGTACAACAACAGCTGC+TGG | - | chr4.4:78124921-78124940 | MS.gene50323:intron | 55.0% | |
| GGACTAACTGCTGCTGCAGT+AGG | - | chr4.4:78125014-78125033 | MS.gene50323:intron | 55.0% | |
| GTAGGTGCAGTAGTGGTAGG+TGG | - | chr4.4:78125032-78125051 | MS.gene50323:intron | 55.0% | |
| GTTAGTCCCGCAATTGCAGC+AGG | + | chr4.4:78125002-78125021 | None:intergenic | 55.0% | |
| TGCTGCAGTAGGTGCAGTAG+TGG | - | chr4.4:78125025-78125044 | MS.gene50323:intron | 55.0% | |
| ! | GTGGGAACTGTCGGTGCTTT+GGG | - | chr4.4:78124951-78124970 | MS.gene50323:intron | 55.0% |
| ! | TGTGGGAACTGTCGGTGCTT+TGG | - | chr4.4:78124950-78124969 | MS.gene50323:intron | 55.0% |
| !! | AACAGCTGCTGGAGTTGGTG+TGG | - | chr4.4:78124932-78124951 | MS.gene50323:intron | 55.0% |
| !! | ACAGCTGCTGGAGTTGGTGT+GGG | - | chr4.4:78124933-78124952 | MS.gene50323:intron | 55.0% |
| !! | GGAGTTGGTGTGGGAACTGT+CGG | - | chr4.4:78124942-78124961 | MS.gene50323:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 78124885 | 78125449 | 78124885 | ID=MS.gene50323 |
| chr4.4 | mRNA | 78124885 | 78125449 | 78124885 | ID=MS.gene50323.t1;Parent=MS.gene50323 |
| chr4.4 | exon | 78125174 | 78125449 | 78125174 | ID=MS.gene50323.t1.exon1;Parent=MS.gene50323.t1 |
| chr4.4 | CDS | 78125174 | 78125449 | 78125174 | ID=cds.MS.gene50323.t1;Parent=MS.gene50323.t1 |
| chr4.4 | exon | 78124885 | 78124929 | 78124885 | ID=MS.gene50323.t1.exon2;Parent=MS.gene50323.t1 |
| chr4.4 | CDS | 78124885 | 78124929 | 78124885 | ID=cds.MS.gene50323.t1;Parent=MS.gene50323.t1 |
| Gene Sequence |
| Protein sequence |
