Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50865.t1 | XP_024634947.1 | 95.6 | 135 | 6 | 0 | 1 | 135 | 258 | 392 | 7.00E-62 | 246.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50865.t1 | A0A3Q7XSX2 | 80.7 | 135 | 26 | 0 | 1 | 135 | 173 | 307 | 1.4e-51 | 211.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene50865 | 0.811095 | 8.31E-51 | -1.69E-46 |
| MS.gene050026 | MS.gene50865 | 0.822786 | 1.97E-53 | -1.69E-46 |
| MS.gene050029 | MS.gene50865 | 0.83036 | 3.07E-55 | -1.69E-46 |
| MS.gene050043 | MS.gene50865 | 0.802394 | 5.73E-49 | -1.69E-46 |
| MS.gene052447 | MS.gene50865 | 0.863981 | 1.69E-64 | -1.69E-46 |
| MS.gene05329 | MS.gene50865 | 0.843752 | 1.15E-58 | -1.69E-46 |
| MS.gene053883 | MS.gene50865 | 0.807187 | 5.71E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene50865 | 0.825774 | 3.90E-54 | -1.69E-46 |
| MS.gene05423 | MS.gene50865 | 0.815479 | 9.05E-52 | -1.69E-46 |
| MS.gene054291 | MS.gene50865 | -0.802856 | 4.60E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene50865 | 0.802209 | 6.25E-49 | -1.69E-46 |
| MS.gene059272 | MS.gene50865 | 0.802964 | 4.37E-49 | -1.69E-46 |
| MS.gene059458 | MS.gene50865 | 0.841617 | 4.26E-58 | -1.69E-46 |
| MS.gene059459 | MS.gene50865 | 0.822647 | 2.12E-53 | -1.69E-46 |
| MS.gene059460 | MS.gene50865 | 0.834069 | 3.71E-56 | -1.69E-46 |
| MS.gene059584 | MS.gene50865 | 0.807093 | 5.98E-50 | -1.69E-46 |
| MS.gene060134 | MS.gene50865 | 0.830163 | 3.43E-55 | -1.69E-46 |
| MS.gene060135 | MS.gene50865 | 0.805055 | 1.61E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50865.t1 | MTR_2g021870 | 94.643 | 56 | 3 | 0 | 80 | 135 | 1 | 56 | 6.41e-32 | 108 |
| MS.gene50865.t1 | MTR_4g125750 | 47.619 | 84 | 44 | 0 | 27 | 110 | 114 | 197 | 2.96e-20 | 82.4 |
| MS.gene50865.t1 | MTR_6g012560 | 54.839 | 62 | 21 | 1 | 81 | 135 | 109 | 170 | 2.36e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGGGGCGTGTGCGATGTA+TGG | 0.383383 | 3.1:-14449322 | MS.gene50865:CDS |
| ACAAGCAGGGTTCTATGTCT+TGG | 0.453913 | 3.1:-14449383 | MS.gene50865:CDS |
| GCGTGATAGAGTTAAGACTA+TGG | 0.458291 | 3.1:-14449244 | MS.gene50865:CDS |
| AGTACAAGGAGCTGCAAAGA+AGG | 0.477092 | 3.1:-14449340 | MS.gene50865:CDS |
| GAAACTCGCACACGTAAAGA+TGG | 0.479558 | 3.1:-14449815 | MS.gene50865:CDS |
| GATAATACAAGCTGCTAGAC+AGG | 0.497499 | 3.1:-14449157 | None:intergenic |
| TCAATCAACTCAAAACAAGC+AGG | 0.514800 | 3.1:-14449397 | MS.gene50865:CDS |
| GTACAAGGAGCTGCAAAGAA+GGG | 0.517138 | 3.1:-14449339 | MS.gene50865:CDS |
| AAGGGGCGTGTGCGATGTAT+GGG | 0.524253 | 3.1:-14449321 | MS.gene50865:CDS |
| ATGTAAATATCTTGACGTCC+AGG | 0.530259 | 3.1:+14449839 | None:intergenic |
| AAGGGAAAAGGAGTGATGCC+TGG | 0.537898 | 3.1:-14449857 | MS.gene50865:CDS |
| GCAGGGTTCTATGTCTTGGA+AGG | 0.592209 | 3.1:-14449379 | MS.gene50865:CDS |
| AAACTCGCACACGTAAAGAT+GGG | 0.619883 | 3.1:-14449814 | MS.gene50865:CDS |
| CAATCAACTCAAAACAAGCA+GGG | 0.628434 | 3.1:-14449396 | MS.gene50865:CDS |
| TACAAGGAGCTGCAAAGAAG+GGG | 0.632795 | 3.1:-14449338 | MS.gene50865:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACAGAATTAAAAACAGT+TGG | + | chr3.1:14449557-14449576 | None:intergenic | 20.0% |
| !! | ACCTAAATCTAAAAAATCAA+AGG | - | chr3.1:14449729-14449748 | MS.gene50865:intron | 20.0% |
| !! | ACTAAAAAAACATAGTAATG+AGG | - | chr3.1:14449591-14449610 | MS.gene50865:intron | 20.0% |
| !! | TTCTTGTAGTAATTATGATT+TGG | - | chr3.1:14449371-14449390 | MS.gene50865:CDS | 20.0% |
| !!! | AACTGTTTTTAATTCTGTTT+TGG | - | chr3.1:14449556-14449575 | MS.gene50865:intron | 20.0% |
| !!! | ACCTTTGATTTTTTAGATTT+AGG | + | chr3.1:14449733-14449752 | None:intergenic | 20.0% |
| !!! | ACTGTTTTTAATTCTGTTTT+GGG | - | chr3.1:14449557-14449576 | MS.gene50865:intron | 20.0% |
| ! | ATACTTTCAAATGGTATAAC+AGG | + | chr3.1:14449330-14449349 | None:intergenic | 25.0% |
| ! | CAAGATATCATACTTTCAAA+TGG | + | chr3.1:14449339-14449358 | None:intergenic | 25.0% |
| ! | TTGTAAGCATCTAACATTTA+TGG | - | chr3.1:14449261-14449280 | MS.gene50865:CDS | 25.0% |
| !! | TGGCTTGTAAAATATATTTC+AGG | - | chr3.1:14449438-14449457 | MS.gene50865:CDS | 25.0% |
| !!! | AATTATGATTTGGTGAGATA+TGG | - | chr3.1:14449381-14449400 | MS.gene50865:CDS | 25.0% |
| !!! | GACATATTTTCTAAAGTACA+AGG | - | chr3.1:14449667-14449686 | MS.gene50865:intron | 25.0% |
| AAAAAACATAGTAATGAGGC+TGG | - | chr3.1:14449595-14449614 | MS.gene50865:intron | 30.0% | |
| ATTAAAAACAGTTGGCAATC+AGG | + | chr3.1:14449549-14449568 | None:intergenic | 30.0% | |
| CAGAAAAACAGAGAATCATT+CGG | + | chr3.1:14449291-14449310 | None:intergenic | 30.0% | |
| !! | TTGATTCAAAGTCGATTTTC+TGG | - | chr3.1:14449826-14449845 | MS.gene50865:CDS | 30.0% |
| !!! | GTAGACTATTGTTTGTTTTG+TGG | - | chr3.1:14449418-14449437 | MS.gene50865:CDS | 30.0% |
| !!! | TTAATTCTGTTTTGGGTTTC+AGG | - | chr3.1:14449564-14449583 | MS.gene50865:intron | 30.0% |
| ATGTAAATATCTTGACGTCC+AGG | + | chr3.1:14449185-14449204 | None:intergenic | 35.0% | |
| CAATCAACTCAAAACAAGCA+GGG | - | chr3.1:14449625-14449644 | MS.gene50865:intron | 35.0% | |
| TCAATCAACTCAAAACAAGC+AGG | - | chr3.1:14449624-14449643 | MS.gene50865:intron | 35.0% | |
| !! | GAAAAGGCATTAGAGTTTCT+TGG | + | chr3.1:14449523-14449542 | None:intergenic | 35.0% |
| AAACTCGCACACGTAAAGAT+GGG | - | chr3.1:14449207-14449226 | MS.gene50865:CDS | 40.0% | |
| AACAATAGTCTACTCACTCC+TGG | + | chr3.1:14449411-14449430 | None:intergenic | 40.0% | |
| GCGTGATAGAGTTAAGACTA+TGG | - | chr3.1:14449777-14449796 | MS.gene50865:intron | 40.0% | |
| TTGGTGAGATATGGAAAACC+AGG | - | chr3.1:14449390-14449409 | MS.gene50865:CDS | 40.0% | |
| ACAAGCAGGGTTCTATGTCT+TGG | - | chr3.1:14449638-14449657 | MS.gene50865:intron | 45.0% | |
| AGTACAAGGAGCTGCAAAGA+AGG | - | chr3.1:14449681-14449700 | MS.gene50865:intron | 45.0% | |
| GAAACTCGCACACGTAAAGA+TGG | - | chr3.1:14449206-14449225 | MS.gene50865:CDS | 45.0% | |
| GTACAAGGAGCTGCAAAGAA+GGG | - | chr3.1:14449682-14449701 | MS.gene50865:intron | 45.0% | |
| GTTGGCAATCAGGCATGAAA+AGG | + | chr3.1:14449539-14449558 | None:intergenic | 45.0% | |
| TACAAGGAGCTGCAAAGAAG+GGG | - | chr3.1:14449683-14449702 | MS.gene50865:intron | 45.0% | |
| AAGGGAAAAGGAGTGATGCC+TGG | - | chr3.1:14449164-14449183 | MS.gene50865:CDS | 50.0% | |
| GCAGGGTTCTATGTCTTGGA+AGG | - | chr3.1:14449642-14449661 | MS.gene50865:intron | 50.0% | |
| ! | AAGGGGCGTGTGCGATGTAT+GGG | - | chr3.1:14449700-14449719 | MS.gene50865:intron | 55.0% |
| ! | GAAGGGGCGTGTGCGATGTA+TGG | - | chr3.1:14449699-14449718 | MS.gene50865:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 14449158 | 14449885 | 14449158 | ID=MS.gene50865 |
| chr3.1 | mRNA | 14449158 | 14449885 | 14449158 | ID=MS.gene50865.t1;Parent=MS.gene50865 |
| chr3.1 | exon | 14449781 | 14449885 | 14449781 | ID=MS.gene50865.t1.exon1;Parent=MS.gene50865.t1 |
| chr3.1 | CDS | 14449781 | 14449885 | 14449781 | ID=cds.MS.gene50865.t1;Parent=MS.gene50865.t1 |
| chr3.1 | exon | 14449158 | 14449457 | 14449158 | ID=MS.gene50865.t1.exon2;Parent=MS.gene50865.t1 |
| chr3.1 | CDS | 14449158 | 14449457 | 14449158 | ID=cds.MS.gene50865.t1;Parent=MS.gene50865.t1 |
| Gene Sequence |
| Protein sequence |
