AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene52583


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52583.t1 MTR_8g090105 69.778 225 67 1 1 225 1 224 3.09e-110 317
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene52583.t1 AT3G57360 46.930 228 98 5 1 224 1 209 4.38e-58 184
MS.gene52583.t1 AT3G02370 48.485 165 79 2 61 224 1 160 3.04e-48 156
MS.gene52583.t1 AT3G02370 48.485 165 79 2 61 224 1 160 1.27e-47 156
MS.gene52583.t1 AT3G02370 48.485 165 79 2 61 224 1 160 1.27e-47 156
MS.gene52583.t1 AT3G02370 48.485 165 79 2 61 224 1 160 1.27e-47 156

Find 41 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAAGATCAGAAGATTTA+AGG 0.117924 7.4:-26026200 None:intergenic
CATTCCATTGATAAGTTATT+TGG 0.155844 7.4:+26026270 MS.gene52583:CDS
GACAAGCACATGAGAAAATT+TGG 0.212952 7.4:-26026375 None:intergenic
GATTTGGCACAGGTATCTTA+TGG 0.264509 7.4:+26025987 MS.gene52583:intron
AAAGTTGATGGTCGGAAGTT+TGG 0.269564 7.4:+26026075 MS.gene52583:CDS
CCTGGCCCAACTTGCAATTC+AGG 0.275772 7.4:+26024887 MS.gene52583:CDS
TTCAAGATCAGAAGATTTAA+GGG 0.330837 7.4:-26026199 None:intergenic
CTTAAATCTTCTGATCTTGA+AGG 0.344176 7.4:+26026201 MS.gene52583:CDS
GAACTATGGAAGAAAAGGTT+TGG 0.353007 7.4:+26024764 None:intergenic
ACCATTATCTAATAGATCTA+AGG 0.353230 7.4:-26026019 None:intergenic
GGAAGATAAACATCAAAATC+TGG 0.353821 7.4:-26026318 None:intergenic
AAGCAGACAATTAGTAGATA+TGG 0.368214 7.4:+26026233 MS.gene52583:CDS
AACTATGGAAGAAAAGGTTT+GGG 0.368802 7.4:+26024765 None:intergenic
GACTTTCTAAACCTCTTGTT+TGG 0.373355 7.4:-26026339 None:intergenic
GCCTTAGATCTATTAGATAA+TGG 0.413209 7.4:+26026018 MS.gene52583:CDS
CTTTACCTGAATTGCAAGTT+GGG 0.426982 7.4:-26024892 None:intergenic
GTCGGAAGTTTGGATGTTGT+TGG 0.440117 7.4:+26026085 MS.gene52583:CDS
TTCGGCGGCATGGTGTTGCT+TGG 0.440523 7.4:+26026154 MS.gene52583:CDS
AGTCTCTTGGCTACATAGTT+CGG 0.448906 7.4:+26026136 MS.gene52583:CDS
CAAGATTTACACTTCAATTG+AGG 0.454593 7.4:+26025731 MS.gene52583:CDS
GTCACCAAATAACTTATCAA+TGG 0.481135 7.4:-26026274 None:intergenic
CCTGAATTGCAAGTTGGGCC+AGG 0.481566 7.4:-26024887 None:intergenic
ACTTTACCTGAATTGCAAGT+TGG 0.486911 7.4:-26024893 None:intergenic
TCGGAAGTTTGGATGTTGTT+GGG 0.490686 7.4:+26026086 MS.gene52583:CDS
TGCAAGTTGGGCCAGGAAGT+TGG 0.490903 7.4:-26024880 None:intergenic
GGCAGATGGAATGAAGAGAT+GGG 0.508846 7.4:+26025645 MS.gene52583:CDS
TTGCAAGATGCAAGTGATGA+TGG 0.517783 7.4:+26024838 MS.gene52583:CDS
GCAGAAGTGTATGAAAAGAA+AGG 0.520348 7.4:+26025672 MS.gene52583:CDS
GGCTACATAGTTCGGCGGCA+TGG 0.523179 7.4:+26026144 MS.gene52583:CDS
AGGCAGATGGAATGAAGAGA+TGG 0.524534 7.4:+26025644 MS.gene52583:CDS
AGGTTTAGAAAGTCTTCTCC+TGG 0.525155 7.4:+26026348 MS.gene52583:CDS
TATAGGCACCTCAAGTCTCT+TGG 0.537490 7.4:+26026123 MS.gene52583:CDS
ATGTTTATCTTCCAAACAAG+AGG 0.541371 7.4:+26026328 MS.gene52583:CDS
ATGAGAAAATTTGGAATACC+AGG 0.550816 7.4:-26026366 None:intergenic
ACACAAAATCGAAAGGCAGA+TGG 0.564175 7.4:+26025631 MS.gene52583:CDS
ACAACTGGAATAGTTCGTAA+TGG 0.568611 7.4:+26025708 MS.gene52583:CDS
ATGGAATGAAGAGATGGGAA+TGG 0.574705 7.4:+26025650 MS.gene52583:CDS
TCCAGGAACACAAAATCGAA+AGG 0.612133 7.4:+26025624 MS.gene52583:intron
CTCTTGGCTACATAGTTCGG+CGG 0.631908 7.4:+26026139 MS.gene52583:CDS
CTATGTAGCCAAGAGACTTG+AGG 0.654136 7.4:-26026131 None:intergenic
ATCAATGGAATGCTTGACTG+TGG 0.707373 7.4:-26026259 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATTTTTTTTTTAATT+TGG + chr7.4:26025157-26025176 MS.gene52583:intron 0.0%
!!! TATTTTTTACTAAATTTACA+GGG + chr7.4:26025272-26025291 MS.gene52583:intron 10.0%
!!! TTATTTTTTACTAAATTTAC+AGG + chr7.4:26025271-26025290 MS.gene52583:intron 10.0%
!! AACAAACAAATTATTTAGTT+AGG + chr7.4:26025858-26025877 MS.gene52583:intron 15.0%
!!! ATGGTTGATATTTTTTTTTT+AGG + chr7.4:26024927-26024946 MS.gene52583:intron 15.0%
!!! ATTATTATGAATTCCTTTTT+CGG - chr7.4:26025918-26025937 None:intergenic 15.0%
!!! GAGTTAATTTTAAGTTTAAA+CGG + chr7.4:26025024-26025043 MS.gene52583:intron 15.0%
!!! TAAATAATTTAATTTAGTGC+TGG + chr7.4:26025336-26025355 MS.gene52583:intron 15.0%
!! AGTAAAAAATAATCATAGAG+AGG - chr7.4:26025263-26025282 None:intergenic 20.0%
!! GAAATGTATAAAAAAGTTGA+TGG + chr7.4:26026063-26026082 MS.gene52583:CDS 20.0%
!!! AGAAGGTATATATAAAAAGA+TGG - chr7.4:26025781-26025800 None:intergenic 20.0%
!!! ATGATGTGTATTTTTTTGTA+TGG + chr7.4:26025224-26025243 MS.gene52583:intron 20.0%
!!! CATAATAATACATTTTTGCT+AGG + chr7.4:26025929-26025948 MS.gene52583:intron 20.0%
!!! TTTTGTATGGATTTTTGTTT+AGG + chr7.4:26025237-26025256 MS.gene52583:intron 20.0%
! ACCATTATCTAATAGATCTA+AGG - chr7.4:26026022-26026041 None:intergenic 25.0%
! ATGAAAAGAAAGGAAAAATG+TGG + chr7.4:26025682-26025701 MS.gene52583:CDS 25.0%
! CATTCCATTGATAAGTTATT+TGG + chr7.4:26026270-26026289 MS.gene52583:CDS 25.0%
! TGAAAAGAAAGGAAAAATGT+GGG + chr7.4:26025683-26025702 MS.gene52583:CDS 25.0%
! TTAAATAAGCCAATCCATAT+AGG + chr7.4:26025518-26025537 MS.gene52583:intron 25.0%
! TTCAAGATCAGAAGATTTAA+GGG - chr7.4:26026202-26026221 None:intergenic 25.0%
!!! TAGGATAAAGTTTTGAAAAC+TGG + chr7.4:26025948-26025967 MS.gene52583:intron 25.0%
!!! TGCATATGTGAATTTTGATT+TGG + chr7.4:26025971-26025990 MS.gene52583:intron 25.0%
!!! TGTATAAAAAAGTTGATGGT+CGG + chr7.4:26026067-26026086 MS.gene52583:CDS 25.0%
!!! TTTTTTTTTTAGGAGAACAG+TGG + chr7.4:26024937-26024956 MS.gene52583:intron 25.0%
AAGCAGACAATTAGTAGATA+TGG + chr7.4:26026233-26026252 MS.gene52583:CDS 30.0%
ACTAAATTTACAGGGACTTA+GGG + chr7.4:26025280-26025299 MS.gene52583:intron 30.0%
ATGTTTATCTTCCAAACAAG+AGG + chr7.4:26026328-26026347 MS.gene52583:CDS 30.0%
CAAGATTTACACTTCAATTG+AGG + chr7.4:26025731-26025750 MS.gene52583:CDS 30.0%
CTTAAATCTTCTGATCTTGA+AGG + chr7.4:26026201-26026220 MS.gene52583:CDS 30.0%
CTTATTTCCATAAACTCTCT+AGG - chr7.4:26025420-26025439 None:intergenic 30.0%
CTTCAAGATCAGAAGATTTA+AGG - chr7.4:26026203-26026222 None:intergenic 30.0%
GCCTTAGATCTATTAGATAA+TGG + chr7.4:26026018-26026037 MS.gene52583:CDS 30.0%
GGAAGATAAACATCAAAATC+TGG - chr7.4:26026321-26026340 None:intergenic 30.0%
GGTAAAGTATGTTTGAACAA+TGG + chr7.4:26024908-26024927 MS.gene52583:intron 30.0%
GTCACCAAATAACTTATCAA+TGG - chr7.4:26026277-26026296 None:intergenic 30.0%
TACTAAATTTACAGGGACTT+AGG + chr7.4:26025279-26025298 MS.gene52583:intron 30.0%
TGGTAAAATTTCACAACAGA+AGG - chr7.4:26025798-26025817 None:intergenic 30.0%
! ATGAGAAAATTTGGAATACC+AGG - chr7.4:26026369-26026388 None:intergenic 30.0%
! CAGAGATATTTTCAGTACTA+AGG - chr7.4:26025544-26025563 None:intergenic 30.0%
! GTTGTGAAATTTTACCAGTA+GGG + chr7.4:26025801-26025820 MS.gene52583:intron 30.0%
! TGTTGTGAAATTTTACCAGT+AGG + chr7.4:26025800-26025819 MS.gene52583:intron 30.0%
!!! TTTTTTGCATTTCATCTTCC+AGG + chr7.4:26025607-26025626 MS.gene52583:intron 30.0%
AACCTATCCTAGAGAGTTTA+TGG + chr7.4:26025410-26025429 MS.gene52583:intron 35.0%
ACAACTGGAATAGTTCGTAA+TGG + chr7.4:26025708-26025727 MS.gene52583:CDS 35.0%
ACTTTACCTGAATTGCAAGT+TGG - chr7.4:26024896-26024915 None:intergenic 35.0%
AGACTGTTTGAAAGAGCTTA+AGG - chr7.4:26025471-26025490 None:intergenic 35.0%
CAAACATGCCTAAGTAACTA+AGG - chr7.4:26025314-26025333 None:intergenic 35.0%
CTTAGTTACTTAGGCATGTT+TGG + chr7.4:26025312-26025331 MS.gene52583:intron 35.0%
CTTTACCTGAATTGCAAGTT+GGG - chr7.4:26024895-26024914 None:intergenic 35.0%
GACAAGCACATGAGAAAATT+TGG - chr7.4:26026378-26026397 None:intergenic 35.0%
GACTTTCTAAACCTCTTGTT+TGG - chr7.4:26026342-26026361 None:intergenic 35.0%
GATGCAAAATTAGCCGAAAA+AGG + chr7.4:26025902-26025921 MS.gene52583:intron 35.0%
TTTCAGTACTAAGGCCTATA+TGG - chr7.4:26025535-26025554 None:intergenic 35.0%
! ATTTTCTCATGTGCTTGTCT+AGG + chr7.4:26026379-26026398 MS.gene52583:CDS 35.0%
! CACACAACTATAAGTTGAGA+AGG + chr7.4:26024994-26025013 MS.gene52583:intron 35.0%
! GCAGAAGTGTATGAAAAGAA+AGG + chr7.4:26025672-26025691 MS.gene52583:CDS 35.0%
! TGATGATGATGATGATGATG+AGG + chr7.4:26024810-26024829 MS.gene52583:CDS 35.0%
!! GAATCTTTAAAGAGTGTCTG+GGG + chr7.4:26025179-26025198 MS.gene52583:intron 35.0%
!! GGAATCTTTAAAGAGTGTCT+GGG + chr7.4:26025178-26025197 MS.gene52583:intron 35.0%
!! TGGAATCTTTAAAGAGTGTC+TGG + chr7.4:26025177-26025196 MS.gene52583:intron 35.0%
!! TTCCATAAACTCTCTAGGAT+AGG - chr7.4:26025415-26025434 None:intergenic 35.0%
!!! TGTGAATTTTGATTTGGCAC+AGG + chr7.4:26025977-26025996 MS.gene52583:intron 35.0%
ACAATGCTAAAGTGAAGCGA+AGG - chr7.4:26025571-26025590 None:intergenic 40.0%
ACACAAAATCGAAAGGCAGA+TGG + chr7.4:26025631-26025650 MS.gene52583:CDS 40.0%
AGGTTTAGAAAGTCTTCTCC+TGG + chr7.4:26026348-26026367 MS.gene52583:CDS 40.0%
AGTCTCTTGGCTACATAGTT+CGG + chr7.4:26026136-26026155 MS.gene52583:CDS 40.0%
ATCAATGGAATGCTTGACTG+TGG - chr7.4:26026262-26026281 None:intergenic 40.0%
ATGCCTAAGTAACTAAGGCA+TGG - chr7.4:26025309-26025328 None:intergenic 40.0%
ATGGAATGAAGAGATGGGAA+TGG + chr7.4:26025650-26025669 MS.gene52583:CDS 40.0%
CAATGCTAAAGTGAAGCGAA+GGG - chr7.4:26025570-26025589 None:intergenic 40.0%
GAAATGCTAACGAGTACTCT+TGG + chr7.4:26025050-26025069 MS.gene52583:intron 40.0%
GATTTGGCACAGGTATCTTA+TGG + chr7.4:26025987-26026006 MS.gene52583:intron 40.0%
GGAAAAATGTGGGTAACAAC+TGG + chr7.4:26025693-26025712 MS.gene52583:CDS 40.0%
GTACTAAGGCCTATATGGAT+TGG - chr7.4:26025530-26025549 None:intergenic 40.0%
TCACCATGCCTTAGTTACTT+AGG + chr7.4:26025303-26025322 MS.gene52583:intron 40.0%
TCCAGGAACACAAAATCGAA+AGG + chr7.4:26025624-26025643 MS.gene52583:intron 40.0%
TCGGAAGTTTGGATGTTGTT+GGG + chr7.4:26026086-26026105 MS.gene52583:CDS 40.0%
TTGCAAGATGCAAGTGATGA+TGG + chr7.4:26024838-26024857 MS.gene52583:CDS 40.0%
! ATGTTGTTGGGAGCTTTTTG+AGG + chr7.4:26026098-26026117 MS.gene52583:CDS 40.0%
! TTGACTTTTCACCAACTTCC+TGG + chr7.4:26024869-26024888 MS.gene52583:CDS 40.0%
!! AAAGTTGATGGTCGGAAGTT+TGG + chr7.4:26026075-26026094 MS.gene52583:CDS 40.0%
AGGCAGATGGAATGAAGAGA+TGG + chr7.4:26025644-26025663 MS.gene52583:CDS 45.0%
CTATGTAGCCAAGAGACTTG+AGG - chr7.4:26026134-26026153 None:intergenic 45.0%
GGCAGATGGAATGAAGAGAT+GGG + chr7.4:26025645-26025664 MS.gene52583:CDS 45.0%
GTCGGAAGTTTGGATGTTGT+TGG + chr7.4:26026085-26026104 MS.gene52583:CDS 45.0%
TATAGGCACCTCAAGTCTCT+TGG + chr7.4:26026123-26026142 MS.gene52583:CDS 45.0%
! GCCTTTCGATTTTGTGTTCC+TGG - chr7.4:26025628-26025647 None:intergenic 45.0%
! GTGCTGTGAATTTTCCCTAC+TGG - chr7.4:26025818-26025837 None:intergenic 45.0%
!!! GGGAGCTTTTTGAGGCTTAT+AGG + chr7.4:26026106-26026125 MS.gene52583:CDS 45.0%
!! ATTAAAACTTATATTATTTA+AGG - chr7.4:26025091-26025110 None:intergenic 5.0%
CTCTTGGCTACATAGTTCGG+CGG + chr7.4:26026139-26026158 MS.gene52583:CDS 50.0%
TGCTAAAGTGAAGCGAAGGG+AGG - chr7.4:26025567-26025586 None:intergenic 50.0%
CCTGGCCCAACTTGCAATTC+AGG + chr7.4:26024887-26024906 MS.gene52583:CDS 55.0%
! CCTGAATTGCAAGTTGGGCC+AGG - chr7.4:26024890-26024909 None:intergenic 55.0%
! TGCAAGTTGGGCCAGGAAGT+TGG - chr7.4:26024883-26024902 None:intergenic 55.0%
GGCTACATAGTTCGGCGGCA+TGG + chr7.4:26026144-26026163 MS.gene52583:CDS 60.0%
!! TTCGGCGGCATGGTGTTGCT+TGG + chr7.4:26026154-26026173 MS.gene52583:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 26024769 26026404 26024769 ID=MS.gene52583
chr7.4 mRNA 26024769 26026404 26024769 ID=MS.gene52583.t1;Parent=MS.gene52583
chr7.4 exon 26024769 26024908 26024769 ID=MS.gene52583.t1.exon1;Parent=MS.gene52583.t1
chr7.4 CDS 26024769 26024908 26024769 ID=cds.MS.gene52583.t1;Parent=MS.gene52583.t1
chr7.4 exon 26025629 26025760 26025629 ID=MS.gene52583.t1.exon2;Parent=MS.gene52583.t1
chr7.4 CDS 26025629 26025760 26025629 ID=cds.MS.gene52583.t1;Parent=MS.gene52583.t1
chr7.4 exon 26025999 26026404 26025999 ID=MS.gene52583.t1.exon3;Parent=MS.gene52583.t1
chr7.4 CDS 26025999 26026404 26025999 ID=cds.MS.gene52583.t1;Parent=MS.gene52583.t1
Gene Sequence

>MS.gene52583

ATGGAAGAAAAGGTTTGGGAATGTTCTTTAAATGAAGATATTGATGATGATGATGATGATGAGGTCTATTTGCAAGATGCAAGTGATGATGGACAAGACTTTGACTTTTCACCAACTTCCTGGCCCAACTTGCAATTCAGGTAAAGTATGTTTGAACAATGGTTGATATTTTTTTTTTAGGAGAACAGTGGTTGATAATATAATTATAATTTCTCAATGATATCACACACAACTATAAGTTGAGAAGGAATAAAAGAGTTAATTTTAAGTTTAAACGGTGAGAAATGCTAACGAGTACTCTTGGATTATTCTTTAAGAACCTTAAATAATATAAGTTTTAATGAAAATTTATGTATTCAATGCATTTGAAAATTGAAATATTAAACTTTTTTAATTTTTTTTTTAATTTGGAATCTTTAAAGAGTGTCTGGGGCACTCGTTAGCAAGATATTTAAATGATGTGTATTTTTTTGTATGGATTTTTGTTTAGGCCTCTCTATGATTATTTTTTACTAAATTTACAGGGACTTAGGGTCACCATGCCTTAGTTACTTAGGCATGTTTGGATAAATAATTTAATTTAGTGCTGGTTTATAAACTATTTCTATGATAAAATGTAAAATAAAGTCAACTGATTTCATAACCTATCCTAGAGAGTTTATGGAAATAAGTTGAAAATGAACATGTCATAAACTGTTTCCTTAAGCTCTTTCAAACAGTCTCACAAATGTTTATGTTAGTAGATAAACTTAAATAAGCCAATCCATATAGGCCTTAGTACTGAAAATATCTCTGCCTCCCTTCGCTTCACTTTAGCATTGTTTTCAAAATGAACTTTTTTTTTGCATTTCATCTTCCAGGAACACAAAATCGAAAGGCAGATGGAATGAAGAGATGGGAATGGCAGAAGTGTATGAAAAGAAAGGAAAAATGTGGGTAACAACTGGAATAGTTCGTAATGGCAAGATTTACACTTCAATTGAGGAAACTTTGTAAGTTATATGATATGCCATCTTTTTATATATACCTTCTGTTGTGAAATTTTACCAGTAGGGAAAATTCACAGCACATAAATCAATATCTCTGTGTAACAAACAAATTATTTAGTTAGGTGTACTATATGATGTGTGTTAGATGCAAAATTAGCCGAAAAAGGAATTCATAATAATACATTTTTGCTAGGATAAAGTTTTGAAAACTGGTGCATATGTGAATTTTGATTTGGCACAGGTATCTTATGGAACTAGAAGCCTTAGATCTATTAGATAATGGTGATAGAAGTATATCTCTAATAGAAATGTATAAAAAAGTTGATGGTCGGAAGTTTGGATGTTGTTGGGAGCTTTTTGAGGCTTATAGGCACCTCAAGTCTCTTGGCTACATAGTTCGGCGGCATGGTGTTGCTTGGAGTTTAAAGAATATTAGAAGTTCCCTTAAATCTTCTGATCTTGAAGGAACAGAAGAAAGCAGACAATTAGTAGATATGGTTACCACAGTCAAGCATTCCATTGATAAGTTATTTGGTGACTTGAAGATTAACGATTTGAAGCCAGATTTTGATGTTTATCTTCCAAACAAGAGGTTTAGAAAGTCTTCTCCTGGTATTCCAAATTTTCTCATGTGCTTGTCTAGGTAA

Protein sequence

>MS.gene52583.t1

MEEKVWECSLNEDIDDDDDDEVYLQDASDDGQDFDFSPTSWPNLQFRNTKSKGRWNEEMGMAEVYEKKGKMWVTTGIVRNGKIYTSIEETLYLMELEALDLLDNGDRSISLIEMYKKVDGRKFGCCWELFEAYRHLKSLGYIVRRHGVAWSLKNIRSSLKSSDLEGTEESRQLVDMVTTVKHSIDKLFGDLKINDLKPDFDVYLPNKRFRKSSPGIPNFLMCLSR