AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54591

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54591.t1 MTR_4g066210 96.989 465 14 0 21 485 63 527 0.0 947
MS.gene54591.t1 MTR_4g066240 87.991 458 55 0 21 478 56 513 0.0 845
MS.gene54591.t1 MTR_4g066240 87.800 459 55 1 21 478 56 514 0.0 840
MS.gene54591.t1 MTR_4g066340 78.391 435 94 0 44 478 6 440 0.0 726
MS.gene54591.t1 MTR_4g066580 68.696 460 144 0 21 480 59 518 0.0 695
MS.gene54591.t1 MTR_2g098790 68.928 457 142 0 21 477 58 514 0.0 686
MS.gene54591.t1 MTR_3g028720 67.174 460 151 0 21 480 58 517 0.0 684
MS.gene54591.t1 MTR_4g066630 68.352 455 144 0 21 475 59 513 0.0 681
MS.gene54591.t1 MTR_2g098520 67.177 457 150 0 21 477 54 510 0.0 679
MS.gene54591.t1 MTR_4g066590 66.811 461 153 0 21 481 53 513 0.0 678
MS.gene54591.t1 MTR_4g066240 87.466 367 46 0 21 387 56 422 0.0 676
MS.gene54591.t1 MTR_6g078450 66.449 459 154 0 21 479 56 514 0.0 676
MS.gene54591.t1 MTR_2g098540 66.958 457 151 0 21 477 51 507 0.0 675
MS.gene54591.t1 MTR_2g098540 66.450 462 155 0 16 477 2 463 0.0 674
MS.gene54591.t1 MTR_6g078330 67.177 457 150 0 21 477 57 513 0.0 666
MS.gene54591.t1 MTR_6g078380 68.709 457 142 1 21 476 56 512 0.0 657
MS.gene54591.t1 MTR_4g066640 64.270 459 160 2 21 478 59 514 0.0 644
MS.gene54591.t1 MTR_5g071520 63.948 466 159 1 21 477 59 524 0.0 616
MS.gene54591.t1 MTR_4g066330 62.745 459 143 2 21 477 61 493 0.0 606
MS.gene54591.t1 MTR_3g028720 67.662 402 130 0 21 422 58 459 0.0 603
MS.gene54591.t1 MTR_4g066630 68.329 401 127 0 21 421 59 459 0.0 602
MS.gene54591.t1 MTR_4g066280 61.656 459 148 2 21 477 61 493 0.0 600
MS.gene54591.t1 MTR_6g078330 67.237 409 134 0 69 477 1 409 0.0 598
MS.gene54591.t1 MTR_6g078450 67.413 402 131 0 21 422 56 457 0.0 594
MS.gene54591.t1 MTR_3g026280 59.300 457 181 2 21 477 42 493 0.0 581
MS.gene54591.t1 MTR_6g078330 65.743 397 134 1 81 477 15 409 0.0 563
MS.gene54591.t1 MTR_4g066580 68.649 370 116 0 21 390 59 428 0.0 561
MS.gene54591.t1 MTR_4g066630 68.392 367 116 0 21 387 59 425 0.0 555
MS.gene54591.t1 MTR_3g028720 67.935 368 118 0 21 388 58 425 0.0 555
MS.gene54591.t1 MTR_4g023000 57.987 457 186 3 21 477 44 494 0.0 554
MS.gene54591.t1 MTR_4g067040 67.514 354 115 0 21 374 54 407 0.0 526
MS.gene54591.t1 MTR_2g043200 57.667 463 162 7 21 477 85 519 0.0 525
MS.gene54591.t1 MTR_4g015420 53.945 469 176 6 21 477 85 525 0.0 520
MS.gene54591.t1 MTR_3g026400 53.173 457 208 4 22 477 27 478 4.11e-180 513
MS.gene54591.t1 MTR_3g026400 53.173 457 208 4 22 477 58 509 9.68e-180 514
MS.gene54591.t1 MTR_4g067040 69.301 329 101 0 21 349 54 382 3.20e-178 505
MS.gene54591.t1 MTR_3g028720 68.389 329 104 0 21 349 58 386 1.01e-177 504
MS.gene54591.t1 MTR_4g015460 54.176 467 175 9 21 476 62 500 1.97e-174 500
MS.gene54591.t1 MTR_4g083000 52.201 477 218 6 4 476 32 502 3.37e-173 497
MS.gene54591.t1 MTR_3g026620 53.509 456 208 3 22 477 59 510 4.38e-173 497
MS.gene54591.t1 MTR_4g066630 69.497 318 97 0 21 338 59 376 1.16e-172 491
MS.gene54591.t1 MTR_5g044000 52.620 458 211 4 21 475 66 520 1.05e-169 488
MS.gene54591.t1 MTR_6g078330 67.500 320 104 0 21 340 57 376 1.51e-164 470
MS.gene54591.t1 MTR_4g066640 62.840 331 119 2 21 350 59 386 7.74e-159 456
MS.gene54591.t1 MTR_4g081530 48.643 479 237 6 10 484 34 507 2.36e-155 452
MS.gene54591.t1 MTR_0110s0040 49.234 457 223 6 22 477 64 512 2.27e-153 446
MS.gene54591.t1 MTR_0110s0030 49.015 457 224 6 22 477 64 512 3.58e-153 446
MS.gene54591.t1 MTR_4g131810 48.690 458 230 5 21 475 48 503 5.13e-153 446
MS.gene54591.t1 MTR_4g083000 51.942 412 190 4 69 476 1 408 1.53e-150 436
MS.gene54591.t1 MTR_2g022760 48.380 463 234 5 21 481 56 515 4.06e-149 436
MS.gene54591.t1 MTR_3g033790 45.992 474 236 7 13 482 29 486 1.80e-145 426
MS.gene54591.t1 MTR_3g033810 45.975 472 240 5 13 482 31 489 2.07e-144 423
MS.gene54591.t1 MTR_4g131800 48.380 463 233 6 21 481 536 994 9.88e-144 438
MS.gene54591.t1 MTR_2g022710 44.227 459 250 5 21 477 55 509 2.23e-139 411
MS.gene54591.t1 MTR_7g068910 44.586 471 239 7 13 477 41 495 2.26e-139 411
MS.gene54591.t1 MTR_2g079550 45.043 464 244 6 22 481 51 507 1.92e-138 409
MS.gene54591.t1 MTR_2g079580 44.828 464 245 6 22 481 53 509 3.19e-138 408
MS.gene54591.t1 MTR_7g033860 46.087 460 233 5 22 479 69 515 1.44e-137 406
MS.gene54591.t1 MTR_7g068910 43.659 481 239 8 13 477 41 505 2.39e-136 404
MS.gene54591.t1 MTR_3g026400 61.279 297 112 2 22 318 58 351 4.66e-133 390
MS.gene54591.t1 MTR_5g068740 44.469 461 235 8 22 478 48 491 1.89e-132 393
MS.gene54591.t1 MTR_4g016920 43.871 465 243 7 22 483 48 497 3.93e-132 392
MS.gene54591.t1 MTR_0108s0030 43.043 460 233 4 22 481 24 454 3.95e-128 380
MS.gene54591.t1 MTR_1g088610 44.639 457 235 7 22 476 48 488 2.11e-126 382
MS.gene54591.t1 MTR_1g088610 41.176 119 63 2 361 478 503 615 4.75e-18 87.4
MS.gene54591.t1 MTR_2g022760 46.747 415 216 5 69 481 1 412 4.77e-126 373
MS.gene54591.t1 MTR_7g068900 45.070 426 198 8 64 476 17 419 1.10e-120 362
MS.gene54591.t1 MTR_2g022710 43.350 406 225 4 74 477 6 408 1.42e-120 359
MS.gene54591.t1 MTR_1g088630 42.283 473 227 10 10 478 4 434 1.59e-120 360
MS.gene54591.t1 MTR_2g022710 42.822 404 226 4 21 422 55 455 3.59e-116 350
MS.gene54591.t1 MTR_2g022710 42.822 404 226 4 21 422 55 455 6.31e-116 350
MS.gene54591.t1 MTR_7g051510 43.750 464 235 8 22 481 96 537 6.46e-116 352
MS.gene54591.t1 MTR_4g016920 42.823 418 222 6 69 483 1 404 8.11e-115 345
MS.gene54591.t1 MTR_7g051610 43.818 461 237 6 17 475 91 531 2.90e-114 347
MS.gene54591.t1 MTR_2g079580 46.612 369 187 5 22 386 53 415 5.42e-114 342
MS.gene54591.t1 MTR_2g022710 44.444 369 200 4 21 387 55 420 6.64e-114 342
MS.gene54591.t1 MTR_7g051650 42.105 456 243 5 22 475 96 532 9.21e-113 343
MS.gene54591.t1 MTR_7g051650 41.667 456 241 5 22 475 96 528 1.64e-111 340
MS.gene54591.t1 MTR_0110s0040 51.757 313 147 3 22 334 64 372 3.17e-110 332
MS.gene54591.t1 MTR_2g022710 43.820 356 195 4 21 374 55 407 4.57e-107 325
MS.gene54591.t1 MTR_7g056747 43.269 416 206 5 63 475 9 397 2.48e-104 317
MS.gene54591.t1 MTR_2g022710 48.837 301 151 3 21 321 55 352 3.27e-100 307
MS.gene54591.t1 MTR_1g088630 48.000 300 126 5 22 320 48 318 6.19e-94 289
MS.gene54591.t1 MTR_4g016920 51.984 252 119 1 69 320 1 250 8.57e-93 284
MS.gene54591.t1 MTR_1g088630 51.417 247 114 3 22 267 48 289 1.96e-83 260
MS.gene54591.t1 MTR_0110s0020 50.216 231 109 4 13 241 53 279 1.74e-74 237
MS.gene54591.t1 MTR_5g069350 41.341 179 52 5 315 477 48 189 1.65e-29 114
MS.gene54591.t1 MTR_4g122980 27.512 418 228 14 65 475 183 532 3.88e-29 121
MS.gene54591.t1 MTR_8g038650 45.000 100 55 0 227 326 57 156 5.89e-24 98.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54591.t1 AT5G44640 55.580 457 197 3 22 477 52 503 0.0 544
MS.gene54591.t1 AT5G42260 56.319 451 191 3 22 471 52 497 0.0 541
MS.gene54591.t1 AT2G44450 54.923 457 199 3 22 477 52 502 0.0 541
MS.gene54591.t1 AT2G44480 53.392 457 212 1 22 478 87 542 0.0 531
MS.gene54591.t1 AT2G44480 53.392 457 212 1 22 478 56 511 0.0 530
MS.gene54591.t1 AT3G60130 54.825 456 200 3 22 476 51 501 0.0 520
MS.gene54591.t1 AT2G25630 54.505 455 184 4 22 475 51 483 1.13e-179 513
MS.gene54591.t1 AT5G24550 51.948 462 217 5 21 478 52 512 1.63e-173 499
MS.gene54591.t1 AT3G60140 49.049 473 236 5 10 479 36 506 1.27e-165 480
MS.gene54591.t1 AT5G24540 51.948 462 217 5 21 478 52 512 5.62e-165 477
MS.gene54591.t1 AT1G26560 49.247 465 222 5 21 478 52 509 1.08e-163 473
MS.gene54591.t1 AT2G44480 51.951 410 196 1 69 478 1 409 1.00e-161 464
MS.gene54591.t1 AT1G51470 50.218 458 214 6 22 477 66 511 4.54e-161 466
MS.gene54591.t1 AT2G44460 51.183 465 220 6 21 481 49 510 9.70e-161 468
MS.gene54591.t1 AT5G54570 50.545 459 220 4 21 475 50 505 2.79e-160 465
MS.gene54591.t1 AT3G60130 53.494 415 187 3 63 476 40 449 6.03e-160 461
MS.gene54591.t1 AT2G44470 47.579 475 236 6 10 477 39 507 1.04e-159 466
MS.gene54591.t1 AT1G47600 49.784 462 210 7 22 477 66 511 2.52e-159 462
MS.gene54591.t1 AT1G47600 49.567 462 210 7 22 477 66 510 2.19e-158 459
MS.gene54591.t1 AT3G60120 49.676 463 222 7 21 477 34 491 4.95e-158 459
MS.gene54591.t1 AT2G44490 48.373 461 232 5 21 476 34 493 2.94e-154 451
MS.gene54591.t1 AT3G18080 48.584 459 226 7 22 478 60 510 3.51e-153 446
MS.gene54591.t1 AT3G60130 52.048 415 182 4 63 476 40 438 3.33e-151 439
MS.gene54591.t1 AT5G25980 48.164 463 223 10 29 482 75 529 1.49e-150 441
MS.gene54591.t1 AT5G36890 48.837 473 227 5 13 482 26 486 4.81e-150 437
MS.gene54591.t1 AT5G36890 48.837 473 227 5 13 482 26 486 8.75e-150 437
MS.gene54591.t1 AT2G32860 46.025 478 253 5 10 483 104 580 1.33e-149 441
MS.gene54591.t1 AT2G32860 46.025 478 252 6 10 483 104 579 2.20e-148 437
MS.gene54591.t1 AT5G26000 48.478 460 213 9 29 475 63 511 3.51e-147 432
MS.gene54591.t1 AT3G18070 45.752 459 229 9 22 477 51 492 5.07e-146 427
MS.gene54591.t1 AT3G03640 45.887 462 244 4 21 477 54 514 2.09e-145 427
MS.gene54591.t1 AT3G18070 45.752 459 235 9 22 477 51 498 9.42e-145 424
MS.gene54591.t1 AT1G61820 47.403 462 237 5 21 479 50 508 2.84e-144 424
MS.gene54591.t1 AT5G54570 50.122 411 198 4 69 475 1 408 1.04e-141 414
MS.gene54591.t1 AT1G02850 46.667 465 221 8 22 480 47 490 2.12e-140 413
MS.gene54591.t1 AT3G09260 45.238 462 248 5 22 480 56 515 1.39e-138 409
MS.gene54591.t1 AT1G75940 45.671 462 241 7 22 475 58 517 4.31e-138 408
MS.gene54591.t1 AT5G24540 50.495 404 196 4 21 421 52 454 6.07e-138 405
MS.gene54591.t1 AT5G54570 50.123 405 196 3 21 422 50 451 1.78e-137 404
MS.gene54591.t1 AT2G44470 47.129 418 209 5 10 421 39 450 1.04e-136 402
MS.gene54591.t1 AT1G02850 45.041 484 216 9 22 476 47 509 5.74e-136 402
MS.gene54591.t1 AT2G44450 52.941 357 161 3 122 477 1 351 7.54e-136 396
MS.gene54591.t1 AT3G21370 44.850 466 239 9 22 476 54 512 6.68e-135 400
MS.gene54591.t1 AT1G02850 44.948 485 216 10 22 476 47 510 3.85e-134 398
MS.gene54591.t1 AT4G27830 42.981 463 243 8 21 479 43 488 6.99e-134 397
MS.gene54591.t1 AT1G66280 45.474 464 243 7 22 480 57 515 7.93e-134 397
MS.gene54591.t1 AT3G60140 48.825 383 192 4 10 390 36 416 9.15e-134 394
MS.gene54591.t1 AT5G28510 43.255 467 255 6 22 480 60 524 2.27e-133 396
MS.gene54591.t1 AT4G21760 43.326 457 248 6 21 475 74 521 2.62e-133 396
MS.gene54591.t1 AT1G52400 45.572 463 238 10 22 476 60 516 2.64e-132 393
MS.gene54591.t1 AT1G52400 45.572 463 238 10 22 476 60 516 2.64e-132 393
MS.gene54591.t1 AT1G61810 43.441 465 257 5 21 482 53 514 3.85e-132 392
MS.gene54591.t1 AT1G66270 44.060 463 251 6 22 480 57 515 9.02e-132 392
MS.gene54591.t1 AT5G25980 48.628 401 191 8 29 422 75 467 1.60e-131 389
MS.gene54591.t1 AT5G25980 48.628 401 191 8 29 422 75 467 1.60e-131 389
MS.gene54591.t1 AT1G02850 45.376 465 200 9 22 480 47 463 1.03e-130 387
MS.gene54591.t1 AT1G66270 43.844 463 250 7 22 480 57 513 1.05e-128 384
MS.gene54591.t1 AT1G60090 42.733 461 238 9 21 476 44 483 1.28e-128 383
MS.gene54591.t1 AT4G22100 43.506 462 235 9 21 476 42 483 1.90e-128 383
MS.gene54591.t1 AT5G26000 47.525 404 192 6 29 422 63 456 6.51e-128 379
MS.gene54591.t1 AT4G21760 42.670 457 241 7 21 475 74 511 1.42e-126 379
MS.gene54591.t1 AT2G44480 57.586 290 121 2 22 311 87 374 1.63e-125 371
MS.gene54591.t1 AT4G27820 42.333 463 244 7 21 479 43 486 1.71e-125 375
MS.gene54591.t1 AT1G61810 43.820 445 244 5 21 462 53 494 2.73e-125 376
MS.gene54591.t1 AT1G60090 42.026 464 244 10 21 476 44 490 8.69e-125 374
MS.gene54591.t1 AT1G45191 41.342 462 249 8 16 476 44 484 1.67e-121 365
MS.gene54591.t1 AT1G45191 40.860 465 253 8 16 476 44 490 9.60e-120 360
MS.gene54591.t1 AT3G62750 41.612 459 236 9 21 475 43 473 3.00e-119 359
MS.gene54591.t1 AT1G45191 40.340 471 252 8 16 476 44 495 4.46e-119 359
MS.gene54591.t1 AT3G62750 41.253 463 240 9 21 479 69 503 6.28e-119 359
MS.gene54591.t1 AT2G44470 48.538 342 168 4 10 345 39 378 7.62e-117 349
MS.gene54591.t1 AT1G45191 39.749 478 257 9 16 476 44 507 2.06e-116 353
MS.gene54591.t1 AT1G61810 42.754 414 231 5 72 482 4 414 1.42e-114 344
MS.gene54591.t1 AT4G27830 42.169 415 221 7 69 479 1 400 1.56e-114 344
MS.gene54591.t1 AT2G44460 49.180 366 180 5 120 481 1 364 2.36e-114 344
MS.gene54591.t1 AT5G48375 40.724 442 204 6 29 468 54 439 2.38e-114 344
MS.gene54591.t1 AT3G62740 38.125 480 256 11 21 484 43 497 1.95e-111 339
MS.gene54591.t1 AT1G02850 45.038 393 173 6 22 386 47 424 5.04e-110 333
MS.gene54591.t1 AT1G52400 44.963 407 211 9 22 421 60 460 1.27e-108 330
MS.gene54591.t1 AT4G27820 41.205 415 226 6 69 479 1 401 6.33e-106 322
MS.gene54591.t1 AT4G27820 41.205 415 226 6 69 479 1 401 6.33e-106 322
MS.gene54591.t1 AT1G61820 44.744 371 200 4 112 479 1 369 1.09e-104 317
MS.gene54591.t1 AT3G62750 41.278 407 208 8 21 424 69 447 1.68e-101 312
MS.gene54591.t1 AT1G61810 42.547 369 207 4 21 386 53 419 1.07e-100 309
MS.gene54591.t1 AT3G62750 40.000 415 222 8 69 479 1 392 1.54e-100 308
MS.gene54591.t1 AT3G62750 40.000 415 222 8 69 479 1 392 1.54e-100 308
MS.gene54591.t1 AT1G60270 49.147 293 142 3 21 313 44 329 2.93e-100 306
MS.gene54591.t1 AT2G44480 47.508 301 157 1 178 478 6 305 1.88e-99 301
MS.gene54591.t1 AT3G18070 45.427 328 170 6 153 477 100 421 1.70e-98 303
MS.gene54591.t1 AT3G18070 47.492 299 151 4 22 320 51 343 5.42e-98 300
MS.gene54591.t1 AT1G51490 46.356 343 163 8 13 349 30 357 5.01e-97 299
MS.gene54591.t1 AT3G62740 38.916 406 218 9 21 421 43 423 2.36e-95 295
MS.gene54591.t1 AT3G62750 44.884 303 149 5 21 322 43 328 6.93e-93 286
MS.gene54591.t1 AT3G62750 44.884 303 149 5 21 322 43 328 6.93e-93 286
MS.gene54591.t1 AT1G45191 44.590 305 161 3 16 320 44 340 1.33e-92 287
MS.gene54591.t1 AT3G18070 45.079 315 164 6 166 477 1 309 1.34e-92 284
MS.gene54591.t1 AT1G45191 44.590 305 161 3 16 320 44 340 1.94e-92 287
MS.gene54591.t1 AT3G62750 44.884 303 149 5 21 322 69 354 3.51e-92 287
MS.gene54591.t1 AT1G60270 49.383 243 120 2 71 313 3 242 1.55e-81 255
MS.gene54591.t1 AT1G61810 48.400 250 127 2 21 270 53 300 6.64e-80 251
MS.gene54591.t1 AT3G18070 43.220 236 130 3 153 386 100 333 2.17e-65 215
MS.gene54591.t1 AT3G18070 42.601 223 124 3 166 386 1 221 1.29e-59 196
MS.gene54591.t1 AT5G16580 37.631 287 164 7 147 432 27 299 3.35e-52 179
MS.gene54591.t1 AT3G06510 26.014 419 237 13 64 476 159 510 7.00e-31 126
MS.gene54591.t1 AT3G06510 25.682 440 246 13 64 476 159 544 3.03e-30 124

Find 109 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGAAAAGGACCAAGTATT+TGG 0.197865 4.3:+37741478 MS.gene54591:CDS
GCATCTCAATATGGTATAAT+AGG 0.209400 4.3:+37743188 MS.gene54591:CDS
AAAACAGAAGACCTGATATT+TGG 0.235314 4.3:-37742961 None:intergenic
GTAATTTACATTACCGAAAA+TGG 0.241625 4.3:+37744091 MS.gene54591:CDS
GACATTTGCTACAAGGAATT+TGG 0.242233 4.3:+37742738 MS.gene54591:CDS
CGATTCTGTAAGTATCTAAA+AGG 0.249841 4.3:-37744252 None:intergenic
TTCGGGAACAGAACCATATT+TGG 0.289048 4.3:+37742887 MS.gene54591:CDS
AACCAGTGTGCTGAGAGTTT+TGG 0.299820 4.3:-37744580 None:intergenic
AATCATAAAGGATTTGAATA+TGG 0.307459 4.3:+37742208 MS.gene54591:CDS
AAGATCCTCGGATTCTTCTT+TGG 0.311879 4.3:-37743623 None:intergenic
TGGTAGTGTGCAGCCAAATA+TGG 0.343520 4.3:-37742900 None:intergenic
CGAGGCCTCGACTTCATGTT+TGG 0.348528 4.3:+37743278 MS.gene54591:CDS
GATTCTTCAAGCGACAGTTT+TGG 0.358175 4.3:-37744229 None:intergenic
AGAAGAATCCGAGGATCTTA+AGG 0.358352 4.3:+37743627 MS.gene54591:CDS
GGACGTTCCTCAAGCCTTAG+AGG 0.360927 4.3:+37742551 MS.gene54591:CDS
TGCATTTATCCGCGAGGATT+AGG 0.361716 4.3:+37744028 MS.gene54591:CDS
TAAAGCCAGATTCCCATTCA+TGG 0.377760 4.3:-37744504 None:intergenic
AAAATAAGAGATGGAGGTAA+TGG 0.386283 4.3:+37742059 MS.gene54591:CDS
AATTGCACAGGAGGTGATTC+GGG 0.389673 4.3:+37742870 MS.gene54591:CDS
GATAAGAGAATCAGTTTGTA+TGG 0.392110 4.3:-37743731 None:intergenic
TGGAAAAGGACCAAGTATTT+GGG 0.404129 4.3:+37741479 MS.gene54591:CDS
TCTGGCTTTAGCTTGCGATT+TGG 0.404401 4.3:+37744517 MS.gene54591:CDS
CAACCTCATCAACGAACTAT+TGG 0.405447 4.3:+37742407 MS.gene54591:CDS
GATAACCATGAATGGGAATC+TGG 0.405714 4.3:+37744499 MS.gene54591:CDS
GAATTGCACAGGAGGTGATT+CGG 0.408321 4.3:+37742869 MS.gene54591:CDS
CATCTCAATATGGTATAATA+GGG 0.411825 4.3:+37743189 MS.gene54591:CDS
AGGTGCATCAGCAGCATAAG+AGG 0.412498 4.3:-37743686 None:intergenic
TGTAGTAGTTTATTCCTTCA+CGG 0.418155 4.3:-37742383 None:intergenic
GGAAGATATTGGAATCATAA+AGG 0.418325 4.3:+37742196 MS.gene54591:intron
AATATGCAGGCATCTCAATA+TGG 0.419382 4.3:+37743179 MS.gene54591:intron
TATGTATAATAGGAAGATAT+TGG 0.421814 4.3:+37742185 MS.gene54591:intron
ATCAGTTTGTATGGTGGGTC+TGG 0.422909 4.3:-37743722 None:intergenic
AGGGAAGGTGTGAATGTAAA+GGG 0.435489 4.3:+37744454 MS.gene54591:intron
ATTATGTTCAAGCTGCAATC+AGG 0.440754 4.3:+37744296 MS.gene54591:CDS
GATCGGAAAAGTCGCGAAGA+TGG 0.446253 4.3:-37741245 None:intergenic
TTTGCAGAAGGAAAGCTTAG+TGG 0.446993 4.3:+37742351 MS.gene54591:intron
ACTATACAAGACCAAATATC+AGG 0.449280 4.3:+37742950 MS.gene54591:CDS
TCTTCTTTCGCTTGAGAGGC+CGG 0.450631 4.3:-37743239 None:intergenic
ATCAACGAACTATTGGCTAA+TGG 0.455297 4.3:+37742414 MS.gene54591:CDS
AGCATGAATGCTTATGCATA+TGG 0.456434 4.3:+37742807 MS.gene54591:CDS
GGATTACCTTACTCTCTCAT+TGG 0.457583 4.3:+37743210 MS.gene54591:CDS
GAAGAATCCGAGGATCTTAA+GGG 0.463005 4.3:+37743628 MS.gene54591:CDS
AAGAGGATGAGTAATAGTTT+AGG 0.463613 4.3:-37743669 None:intergenic
CACCGGGTCGATGTTCAGAT+TGG 0.463655 4.3:+37742838 MS.gene54591:CDS
GCGATTTGGACTCGTATTTG+TGG 0.465681 4.3:+37744531 MS.gene54591:CDS
ACCGTCATACTCATCCTCTA+AGG 0.470920 4.3:-37742565 None:intergenic
TGCATATGGGAAGTTTGCAC+CGG 0.482317 4.3:+37742821 MS.gene54591:CDS
CATCGTGATGGCAAGCCCCT+TGG 0.493086 4.3:+37743859 MS.gene54591:CDS
CAGGGAAGGTGTGAATGTAA+AGG 0.498317 4.3:+37744453 MS.gene54591:intron
CTCATACCAATGAGAGAGTA+AGG 0.499326 4.3:-37743216 None:intergenic
AACATCTTCTTTCGCTTGAG+AGG 0.502079 4.3:-37743243 None:intergenic
TTGGTGATAGAGTGAAACAT+TGG 0.506776 4.3:+37742757 MS.gene54591:CDS
CAGCAGCATGAGCAAGAAGT+TGG 0.509755 4.3:-37742920 None:intergenic
TATATGGATCCAATCACAAA+AGG 0.510867 4.3:+37743547 MS.gene54591:CDS
CATTGTTGGATAACCATGAA+TGG 0.514197 4.3:+37744491 MS.gene54591:CDS
CTTTGGAGAACGTTGGTAGT+CGG 0.520304 4.3:-37743606 None:intergenic
ATTGTTGGATAACCATGAAT+GGG 0.522990 4.3:+37744492 MS.gene54591:CDS
CTATGCTGACATTTGCTACA+AGG 0.528057 4.3:+37742731 MS.gene54591:CDS
TGGTTAAATCTGAATTGCAC+AGG 0.528273 4.3:+37742858 MS.gene54591:CDS
ACTCTCTCATTGGTATGAGC+CGG 0.532738 4.3:+37743220 MS.gene54591:CDS
TGGCAAGCCCCTTGGTCCAA+TGG 0.533454 4.3:+37743867 MS.gene54591:CDS
GCAGCATTTGAAGATGGAAA+AGG 0.538475 4.3:+37741465 MS.gene54591:CDS
AGAGAATCAGTTTGTATGGT+GGG 0.539142 4.3:-37743727 None:intergenic
GATTCTGTAAGTATCTAAAA+GGG 0.541771 4.3:-37744251 None:intergenic
AAAAGCATGCAATCTTTGGT+TGG 0.542865 4.3:+37743580 MS.gene54591:CDS
AATATATACCATTGGACCAA+GGG 0.544956 4.3:-37743875 None:intergenic
TCGATGACAGTTCATCGTGA+TGG 0.548253 4.3:+37743847 MS.gene54591:intron
GCCTCGACTTCATGTTTGGA+TGG 0.549073 4.3:+37743282 MS.gene54591:CDS
GTGAAAGTATCCCAAATACT+TGG 0.559968 4.3:-37741489 None:intergenic
TAGCCAATAGTTCGTTGATG+AGG 0.565702 4.3:-37742410 None:intergenic
ATATGCAGTCTGCTTCAAGT+TGG 0.568943 4.3:+37744002 MS.gene54591:intron
CGAAGATGGAGTAGTGAAGA+AGG 0.570282 4.3:-37741231 None:intergenic
CGACGCATATCATCGGTACA+AGG 0.572001 4.3:+37742094 MS.gene54591:CDS
GCATTCATGCTCACACTCCA+TGG 0.576199 4.3:-37742795 None:intergenic
GAAACAGGTCCGATTGAGAT+CGG 0.577440 4.3:-37741262 None:intergenic
GAAGGTGCAGCATTTGAAGA+TGG 0.577839 4.3:+37741459 MS.gene54591:CDS
CAATGGAAAAGAGTCACATA+TGG 0.580739 4.3:-37742528 None:intergenic
GATTCTTCTTTGGAGAACGT+TGG 0.587362 4.3:-37743613 None:intergenic
TACTCATCCTCTAAGGCTTG+AGG 0.594134 4.3:-37742558 None:intergenic
TAGCCGACGACGCATATCAT+CGG 0.598194 4.3:+37742087 MS.gene54591:CDS
AAATATATACCATTGGACCA+AGG 0.598574 4.3:-37743876 None:intergenic
CTTGAACATAATAAAGATGA+CGG 0.602302 4.3:-37744285 None:intergenic
GGATTACTTTGAATGAACCA+TGG 0.603853 4.3:+37742778 MS.gene54591:CDS
AGGCTTGAGGAACGTCCCAA+TGG 0.604842 4.3:-37742545 None:intergenic
GTTTAGGCCTAGAAAATCGA+AGG 0.606609 4.3:-37743653 None:intergenic
GCATGAATGCTTATGCATAT+GGG 0.610049 4.3:+37742808 MS.gene54591:CDS
GAAGGACCCCTTAAGATCCT+CGG 0.611639 4.3:-37743635 None:intergenic
AACCAATCTGAACATCGACC+CGG 0.611784 4.3:-37742840 None:intergenic
GGTGTCACTCACACAATCCG+AGG 0.616673 4.3:-37742597 None:intergenic
GGTCTGGCATCGCGTAAGTG+AGG 0.619875 4.3:-37743706 None:intergenic
GCATATGGGAAGTTTGCACC+GGG 0.626791 4.3:+37742822 MS.gene54591:CDS
AAGAGAATCAGTTTGTATGG+TGG 0.627183 4.3:-37743728 None:intergenic
GCCTTAGAGGATGAGTATGA+CGG 0.628770 4.3:+37742564 MS.gene54591:CDS
GCAGAAGGAAAGCTTAGTGG+AGG 0.637778 4.3:+37742354 MS.gene54591:intron
ACCCAAAACTCTCAGCACAC+TGG 0.639555 4.3:+37744578 MS.gene54591:CDS
ATATATACCATTGGACCAAG+GGG 0.639799 4.3:-37743874 None:intergenic
AGTGGAGGTGTAAACCGTGA+AGG 0.641058 4.3:+37742369 MS.gene54591:CDS
ACCATCCAAACATGAAGTCG+AGG 0.641696 4.3:-37743283 None:intergenic
AACAGTTGTCCTAATCCTCG+CGG 0.657090 4.3:-37744037 None:intergenic
TAGCACTCTAGACCAAGCAA+TGG 0.660595 4.3:-37742246 None:intergenic
GTGTCACTCACACAATCCGA+GGG 0.662250 4.3:-37742596 None:intergenic
TTAAATCTGAATTGCACAGG+AGG 0.663041 4.3:+37742861 MS.gene54591:CDS
GTACCGATGATATGCGTCGT+CGG 0.669538 4.3:-37742090 None:intergenic
TTTCGGTAATGTAAATTACG+GGG 0.671497 4.3:-37744087 None:intergenic
AAGAATCCGAGGATCTTAAG+GGG 0.674534 4.3:+37743629 MS.gene54591:CDS
GTTCTCCAAAGAAGAATCCG+AGG 0.684000 4.3:+37743618 MS.gene54591:CDS
ACTTTCACTCATGAACACCC+AGG 0.690909 4.3:+37741504 MS.gene54591:CDS
TGGTTATGCATTTATCCGCG+AGG 0.715115 4.3:+37744022 MS.gene54591:CDS
GATGTTGATGCTGCACAACG+AGG 0.729333 4.3:+37743260 MS.gene54591:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAATATCATGTTTTTTT+TGG + chr4.3:37741568-37741587 MS.gene54591:intron 10.0%
!!! ACTATTTTTTTTTTTTGAAA+AGG - chr4.3:37741853-37741872 None:intergenic 10.0%
!! AATGATAAAATATATACCAT+TGG - chr4.3:37743886-37743905 None:intergenic 15.0%
!! ACTGCATATAATTAAAAAAA+AGG - chr4.3:37743991-37744010 None:intergenic 15.0%
!! AGCAATATATTATTCAAATT+CGG + chr4.3:37742988-37743007 MS.gene54591:intron 15.0%
!! TCTCATATATATTAATGAAA+TGG + chr4.3:37742027-37742046 MS.gene54591:intron 15.0%
!!! ATTTCAAAATTTTGGTTATT+TGG - chr4.3:37743162-37743181 None:intergenic 15.0%
!! AATCATAAAGGATTTGAATA+TGG + chr4.3:37742208-37742227 MS.gene54591:CDS 20.0%
!! AGAAGTAATTAAAAATAACC+TGG - chr4.3:37741525-37741544 None:intergenic 20.0%
!! ATAGGTAAATGTAAAAATGA+AGG - chr4.3:37744414-37744433 None:intergenic 20.0%
!! GAAGTAATTAAAAATAACCT+GGG - chr4.3:37741524-37741543 None:intergenic 20.0%
!! TAGATTACTCTATGTATAAT+AGG + chr4.3:37742175-37742194 MS.gene54591:intron 20.0%
!! TAGGTAAATGTAAAAATGAA+GGG - chr4.3:37744413-37744432 None:intergenic 20.0%
!! TATAAATCACCAATGTATAA+GGG - chr4.3:37742303-37742322 None:intergenic 20.0%
!! TATGTATAATAGGAAGATAT+TGG + chr4.3:37742185-37742204 MS.gene54591:intron 20.0%
!! TATTCATAAAGGTCAATAAT+AGG - chr4.3:37744432-37744451 None:intergenic 20.0%
!! TCAATCATAAATAATCATTG+AGG + chr4.3:37742485-37742504 MS.gene54591:intron 20.0%
!!! AATAGAGTTATTACCATTTT+CGG - chr4.3:37744107-37744126 None:intergenic 20.0%
!!! ATGTAAACTTCATTTGATTT+TGG + chr4.3:37743375-37743394 MS.gene54591:intron 20.0%
!!! ATTTTCGGTAATGTAAATTA+CGG - chr4.3:37744092-37744111 None:intergenic 20.0%
!!! CATTTTGCATTTTTTTCTAT+AGG + chr4.3:37743524-37743543 MS.gene54591:intron 20.0%
!!! CATTTTTTTCTATAGGTATA+TGG + chr4.3:37743531-37743550 MS.gene54591:intron 20.0%
!!! GATTTGTTTATGACTATTTA+GGG + chr4.3:37742637-37742656 MS.gene54591:intron 20.0%
!!! TTAAAAATTTTGTAGTACGA+AGG + chr4.3:37741441-37741460 MS.gene54591:intron 20.0%
! AAATGTTGCACTAAATTGAA+TGG - chr4.3:37741829-37741848 None:intergenic 25.0%
! AAGTGACAAAATCAATTGAT+AGG + chr4.3:37744187-37744206 MS.gene54591:intron 25.0%
! AGTAGCTAAAACTTAATTAG+AGG - chr4.3:37744345-37744364 None:intergenic 25.0%
! ATTGACCTTTATGAATAACA+GGG + chr4.3:37744435-37744454 MS.gene54591:intron 25.0%
! CATCTCAATATGGTATAATA+GGG + chr4.3:37743189-37743208 MS.gene54591:CDS 25.0%
! CTATAAATCACCAATGTATA+AGG - chr4.3:37742304-37742323 None:intergenic 25.0%
! CTTGAACATAATAAAGATGA+CGG - chr4.3:37744288-37744307 None:intergenic 25.0%
! GAAACTCTATCTCAATATAA+CGG + chr4.3:37741693-37741712 MS.gene54591:intron 25.0%
! GATTCTGTAAGTATCTAAAA+GGG - chr4.3:37744254-37744273 None:intergenic 25.0%
! GTAATTTACATTACCGAAAA+TGG + chr4.3:37744091-37744110 MS.gene54591:CDS 25.0%
! TAAATCGTTAGATTAAGAGA+GGG + chr4.3:37741671-37741690 MS.gene54591:intron 25.0%
! TATTGACCTTTATGAATAAC+AGG + chr4.3:37744434-37744453 MS.gene54591:intron 25.0%
! TTACAAAACTATGACTTACT+TGG - chr4.3:37742272-37742291 None:intergenic 25.0%
! TTCATATTACTTTGCACAAA+TGG - chr4.3:37743468-37743487 None:intergenic 25.0%
!! CGATTTGTTTATGACTATTT+AGG + chr4.3:37742636-37742655 MS.gene54591:intron 25.0%
!! CTTTTCCTTAATGATGTATT+TGG + chr4.3:37741907-37741926 MS.gene54591:intron 25.0%
!! TTTTCGGTAATGTAAATTAC+GGG - chr4.3:37744091-37744110 None:intergenic 25.0%
!!! ACCAAAATTTTGAAATATGC+AGG + chr4.3:37743166-37743185 MS.gene54591:intron 25.0%
!!! AGTTGATAGGAAATTTATGA+TGG - chr4.3:37742330-37742349 None:intergenic 25.0%
!!! AGTTTTAGCTACTGATTTAT+AGG + chr4.3:37744352-37744371 MS.gene54591:intron 25.0%
!!! CATGTTTTTTTTGGTAACAT+GGG + chr4.3:37741577-37741596 MS.gene54591:intron 25.0%
!!! TACTTATTTTCATATCCATG+TGG + chr4.3:37742459-37742478 MS.gene54591:intron 25.0%
!!! TCATGTTTTTTTTGGTAACA+TGG + chr4.3:37741576-37741595 MS.gene54591:intron 25.0%
AAAACAGAAGACCTGATATT+TGG - chr4.3:37742964-37742983 None:intergenic 30.0%
AAAATAAGAGATGGAGGTAA+TGG + chr4.3:37742059-37742078 MS.gene54591:CDS 30.0%
AAATATATACCATTGGACCA+AGG - chr4.3:37743879-37743898 None:intergenic 30.0%
AAGAGGATGAGTAATAGTTT+AGG - chr4.3:37743672-37743691 None:intergenic 30.0%
AATATATACCATTGGACCAA+GGG - chr4.3:37743878-37743897 None:intergenic 30.0%
AATATTCCTCCCTTATACAT+TGG + chr4.3:37742291-37742310 MS.gene54591:intron 30.0%
ACTATACAAGACCAAATATC+AGG + chr4.3:37742950-37742969 MS.gene54591:CDS 30.0%
ACTTGATCTTCTGTAATAGA+AGG + chr4.3:37743049-37743068 MS.gene54591:intron 30.0%
ATATTATTTGGACATGTTGC+AGG - chr4.3:37741609-37741628 None:intergenic 30.0%
ATTGTTGGATAACCATGAAT+GGG + chr4.3:37744492-37744511 MS.gene54591:CDS 30.0%
ATTTATGATTGACAACCACA+TGG - chr4.3:37742477-37742496 None:intergenic 30.0%
CAGATATGATCATGATAATC+TGG + chr4.3:37743342-37743361 MS.gene54591:intron 30.0%
CGATTCTGTAAGTATCTAAA+AGG - chr4.3:37744255-37744274 None:intergenic 30.0%
CTTGATCTTCTGTAATAGAA+GGG + chr4.3:37743050-37743069 MS.gene54591:intron 30.0%
CTTGCAGAAAAAATAAGAGA+TGG + chr4.3:37742050-37742069 MS.gene54591:intron 30.0%
GATAAGAGAATCAGTTTGTA+TGG - chr4.3:37743734-37743753 None:intergenic 30.0%
GCATCTCAATATGGTATAAT+AGG + chr4.3:37743188-37743207 MS.gene54591:CDS 30.0%
GGAAGATATTGGAATCATAA+AGG + chr4.3:37742196-37742215 MS.gene54591:intron 30.0%
GTAAATCGTTAGATTAAGAG+AGG + chr4.3:37741670-37741689 MS.gene54591:intron 30.0%
TATATGGATCCAATCACAAA+AGG + chr4.3:37743547-37743566 MS.gene54591:CDS 30.0%
TGTAGTAGTTTATTCCTTCA+CGG - chr4.3:37742386-37742405 None:intergenic 30.0%
TTCATTATGCCCTAAATGAA+CGG - chr4.3:37741759-37741778 None:intergenic 30.0%
TTGCACCAAATACATCATTA+AGG - chr4.3:37741915-37741934 None:intergenic 30.0%
TTTCGGTAATGTAAATTACG+GGG - chr4.3:37744090-37744109 None:intergenic 30.0%
! AATCGCTCTAGATTGAAAAT+TGG - chr4.3:37742621-37742640 None:intergenic 30.0%
! ATATGTGACTCTTTTCCATT+GGG + chr4.3:37742530-37742549 MS.gene54591:CDS 30.0%
! ATATTTTGCATGGTCATTGT+TGG + chr4.3:37744477-37744496 MS.gene54591:CDS 30.0%
! ATGTAAAGGGATATTTTGCA+TGG + chr4.3:37744467-37744486 MS.gene54591:CDS 30.0%
! CTTATAGATTTTCCATTGCT+TGG + chr4.3:37742234-37742253 MS.gene54591:CDS 30.0%
! GCCTGCATATTTCAAAATTT+TGG - chr4.3:37743170-37743189 None:intergenic 30.0%
! TTCATTTAGGGCATAATGAA+CGG + chr4.3:37741759-37741778 MS.gene54591:intron 30.0%
!! TCTCGTGTTATTTGAATTTC+AGG - chr4.3:37743030-37743049 None:intergenic 30.0%
!!! TTTCTTGGCAAGATTTTTTG+AGG - chr4.3:37744615-37744634 None:intergenic 30.0%
AAATCACCAATGTATAAGGG+AGG - chr4.3:37742300-37742319 None:intergenic 35.0%
AAGAGAATCAGTTTGTATGG+TGG - chr4.3:37743731-37743750 None:intergenic 35.0%
AATATGCAGGCATCTCAATA+TGG + chr4.3:37743179-37743198 MS.gene54591:intron 35.0%
AATTCATGCCCGTTCATTTA+GGG + chr4.3:37741747-37741766 MS.gene54591:intron 35.0%
ACACAAAAGCTACAAATGAG+AGG - chr4.3:37743936-37743955 None:intergenic 35.0%
ACCTTCCCTGTTATTCATAA+AGG - chr4.3:37744443-37744462 None:intergenic 35.0%
ACCTTTATGAATAACAGGGA+AGG + chr4.3:37744439-37744458 MS.gene54591:intron 35.0%
AGAGAATCAGTTTGTATGGT+GGG - chr4.3:37743730-37743749 None:intergenic 35.0%
AGCAAAACCTTATGCTATGT+TGG - chr4.3:37741993-37742012 None:intergenic 35.0%
AGCATGAATGCTTATGCATA+TGG + chr4.3:37742807-37742826 MS.gene54591:CDS 35.0%
ATATATACCATTGGACCAAG+GGG - chr4.3:37743877-37743896 None:intergenic 35.0%
ATCATTGAGGAGAATAATGC+AGG + chr4.3:37742498-37742517 MS.gene54591:intron 35.0%
ATGGAAAAGGACCAAGTATT+TGG + chr4.3:37741478-37741497 MS.gene54591:CDS 35.0%
ATTATGTTCAAGCTGCAATC+AGG + chr4.3:37744296-37744315 MS.gene54591:CDS 35.0%
CAATGGAAAAGAGTCACATA+TGG - chr4.3:37742531-37742550 None:intergenic 35.0%
CATTGTTGGATAACCATGAA+TGG + chr4.3:37744491-37744510 MS.gene54591:CDS 35.0%
GCAGAAAAAATAAGAGATGG+AGG + chr4.3:37742053-37742072 MS.gene54591:intron 35.0%
GCATGAATGCTTATGCATAT+GGG + chr4.3:37742808-37742827 MS.gene54591:CDS 35.0%
GGATTACTTTGAATGAACCA+TGG + chr4.3:37742778-37742797 MS.gene54591:CDS 35.0%
TCATTATGCCCTAAATGAAC+GGG - chr4.3:37741758-37741777 None:intergenic 35.0%
TCCTATCAACTATTTGCAGA+AGG + chr4.3:37742339-37742358 MS.gene54591:intron 35.0%
TCCTTCTGCAAATAGTTGAT+AGG - chr4.3:37742343-37742362 None:intergenic 35.0%
TGGAAAAGGACCAAGTATTT+GGG + chr4.3:37741479-37741498 MS.gene54591:CDS 35.0%
TGGTTAAATCTGAATTGCAC+AGG + chr4.3:37742858-37742877 MS.gene54591:CDS 35.0%
TTAAACTTAGTGCAACTTGC+TGG + chr4.3:37741884-37741903 MS.gene54591:intron 35.0%
TTAAATCTGAATTGCACAGG+AGG + chr4.3:37742861-37742880 MS.gene54591:CDS 35.0%
! AAAAGCATGCAATCTTTGGT+TGG + chr4.3:37743580-37743599 MS.gene54591:CDS 35.0%
! ACCAAAGATTGCATGCTTTT+TGG - chr4.3:37743580-37743599 None:intergenic 35.0%
! ATCAACGAACTATTGGCTAA+TGG + chr4.3:37742414-37742433 MS.gene54591:CDS 35.0%
! ATCTTGCAGAGACGATTTTA+GGG + chr4.3:37742707-37742726 MS.gene54591:intron 35.0%
! CATATGTGACTCTTTTCCAT+TGG + chr4.3:37742529-37742548 MS.gene54591:CDS 35.0%
! GACATTTGCTACAAGGAATT+TGG + chr4.3:37742738-37742757 MS.gene54591:CDS 35.0%
! GGATAGTTTCCTTTTGTGAT+TGG - chr4.3:37743559-37743578 None:intergenic 35.0%
! TAAGGTTTTGCTTGCATGAA+TGG + chr4.3:37742001-37742020 MS.gene54591:intron 35.0%
! TCCAAAAAGCATGCAATCTT+TGG + chr4.3:37743576-37743595 MS.gene54591:CDS 35.0%
! TTGGTGATAGAGTGAAACAT+TGG + chr4.3:37742757-37742776 MS.gene54591:CDS 35.0%
!! CAAGGTATGTAGTTTTGCAA+AGG + chr4.3:37742112-37742131 MS.gene54591:intron 35.0%
!! GTGAAAGTATCCCAAATACT+TGG - chr4.3:37741492-37741511 None:intergenic 35.0%
AACAGCTTCTTCTGCATTTC+AGG + chr4.3:37741305-37741324 MS.gene54591:intron 40.0%
AACATCTTCTTTCGCTTGAG+AGG - chr4.3:37743246-37743265 None:intergenic 40.0%
AAGAATCCGAGGATCTTAAG+GGG + chr4.3:37743629-37743648 MS.gene54591:CDS 40.0%
AAGATCCTCGGATTCTTCTT+TGG - chr4.3:37743626-37743645 None:intergenic 40.0%
AGAAGAATCCGAGGATCTTA+AGG + chr4.3:37743627-37743646 MS.gene54591:CDS 40.0%
AGCTCCTTTCTTTATGCATG+TGG + chr4.3:37741790-37741809 MS.gene54591:intron 40.0%
ATATGCAGTCTGCTTCAAGT+TGG + chr4.3:37744002-37744021 MS.gene54591:intron 40.0%
CAACCTCATCAACGAACTAT+TGG + chr4.3:37742407-37742426 MS.gene54591:CDS 40.0%
CTCATACCAATGAGAGAGTA+AGG - chr4.3:37743219-37743238 None:intergenic 40.0%
GAAGAATCCGAGGATCTTAA+GGG + chr4.3:37743628-37743647 MS.gene54591:CDS 40.0%
GAATTCATGCCCGTTCATTT+AGG + chr4.3:37741746-37741765 MS.gene54591:intron 40.0%
GATAACCATGAATGGGAATC+TGG + chr4.3:37744499-37744518 MS.gene54591:CDS 40.0%
GATCCCACATGCATAAAGAA+AGG - chr4.3:37741797-37741816 None:intergenic 40.0%
GCAGCATTTGAAGATGGAAA+AGG + chr4.3:37741465-37741484 MS.gene54591:CDS 40.0%
GCTCCTTTCTTTATGCATGT+GGG + chr4.3:37741791-37741810 MS.gene54591:intron 40.0%
GGATTACCTTACTCTCTCAT+TGG + chr4.3:37743210-37743229 MS.gene54591:CDS 40.0%
GTTTAGGCCTAGAAAATCGA+AGG - chr4.3:37743656-37743675 None:intergenic 40.0%
TAAAGCCAGATTCCCATTCA+TGG - chr4.3:37744507-37744526 None:intergenic 40.0%
TAAGTAGCTTCTGAATGCCT+TGG + chr4.3:37741963-37741982 MS.gene54591:intron 40.0%
TAGCCAATAGTTCGTTGATG+AGG - chr4.3:37742413-37742432 None:intergenic 40.0%
TATGCTATGTTGGATCTCCA+AGG - chr4.3:37741983-37742002 None:intergenic 40.0%
TCTGTAATAGAAGGGCAAGT+CGG + chr4.3:37743058-37743077 MS.gene54591:intron 40.0%
TGGAGATCCAACATAGCATA+AGG + chr4.3:37741983-37742002 MS.gene54591:intron 40.0%
TTCGGGAACAGAACCATATT+TGG + chr4.3:37742887-37742906 MS.gene54591:CDS 40.0%
TTTGCAGAAGGAAAGCTTAG+TGG + chr4.3:37742351-37742370 MS.gene54591:intron 40.0%
! AGGGAAGGTGTGAATGTAAA+GGG + chr4.3:37744454-37744473 MS.gene54591:intron 40.0%
! CTATGCTGACATTTGCTACA+AGG + chr4.3:37742731-37742750 MS.gene54591:CDS 40.0%
! GATCTTGCAGAGACGATTTT+AGG + chr4.3:37742706-37742725 MS.gene54591:intron 40.0%
! GATTCTTCAAGCGACAGTTT+TGG - chr4.3:37744232-37744251 None:intergenic 40.0%
! GATTCTTCTTTGGAGAACGT+TGG - chr4.3:37743616-37743635 None:intergenic 40.0%
!!! ATGACGGTTTTTTAAGCCCT+CGG + chr4.3:37742580-37742599 MS.gene54591:CDS 40.0%
AAATCGTCTCTGCAAGATCG+CGG - chr4.3:37742705-37742724 None:intergenic 45.0%
AACAGTTGTCCTAATCCTCG+CGG - chr4.3:37744040-37744059 None:intergenic 45.0%
AACCAATCTGAACATCGACC+CGG - chr4.3:37742843-37742862 None:intergenic 45.0%
AATTGCACAGGAGGTGATTC+GGG + chr4.3:37742870-37742889 MS.gene54591:CDS 45.0%
ACCATCCAAACATGAAGTCG+AGG - chr4.3:37743286-37743305 None:intergenic 45.0%
ACCGTCATACTCATCCTCTA+AGG - chr4.3:37742568-37742587 None:intergenic 45.0%
ACTCTCTCATTGGTATGAGC+CGG + chr4.3:37743220-37743239 MS.gene54591:CDS 45.0%
ACTTTCACTCATGAACACCC+AGG + chr4.3:37741504-37741523 MS.gene54591:CDS 45.0%
CGAAGATGGAGTAGTGAAGA+AGG - chr4.3:37741234-37741253 None:intergenic 45.0%
GAAACAGGTCCGATTGAGAT+CGG - chr4.3:37741265-37741284 None:intergenic 45.0%
GAATTGCACAGGAGGTGATT+CGG + chr4.3:37742869-37742888 MS.gene54591:CDS 45.0%
GCCTTAGAGGATGAGTATGA+CGG + chr4.3:37742564-37742583 MS.gene54591:CDS 45.0%
GTTCTCCAAAGAAGAATCCG+AGG + chr4.3:37743618-37743637 MS.gene54591:CDS 45.0%
TAGCACTCTAGACCAAGCAA+TGG - chr4.3:37742249-37742268 None:intergenic 45.0%
TCGATGACAGTTCATCGTGA+TGG + chr4.3:37743847-37743866 MS.gene54591:intron 45.0%
TGCATATGGGAAGTTTGCAC+CGG + chr4.3:37742821-37742840 MS.gene54591:CDS 45.0%
TGCATTTATCCGCGAGGATT+AGG + chr4.3:37744028-37744047 MS.gene54591:CDS 45.0%
TGGTTATGCATTTATCCGCG+AGG + chr4.3:37744022-37744041 MS.gene54591:CDS 45.0%
! AACCAGTGTGCTGAGAGTTT+TGG - chr4.3:37744583-37744602 None:intergenic 45.0%
! AAGGGGTCCTTCGATTTTCT+AGG + chr4.3:37743646-37743665 MS.gene54591:CDS 45.0%
! ACCAGTGTGCTGAGAGTTTT+GGG - chr4.3:37744582-37744601 None:intergenic 45.0%
! CAGGGAAGGTGTGAATGTAA+AGG + chr4.3:37744453-37744472 MS.gene54591:intron 45.0%
! GAAGGTGCAGCATTTGAAGA+TGG + chr4.3:37741459-37741478 MS.gene54591:CDS 45.0%
! GCGACTTTTCCGATCTCAAT+CGG + chr4.3:37741253-37741272 MS.gene54591:CDS 45.0%
! GCGATTTGGACTCGTATTTG+TGG + chr4.3:37744531-37744550 MS.gene54591:CDS 45.0%
! TGGTAGTGTGCAGCCAAATA+TGG - chr4.3:37742903-37742922 None:intergenic 45.0%
!! ATCAGTTTGTATGGTGGGTC+TGG - chr4.3:37743725-37743744 None:intergenic 45.0%
!! CTTTGGAGAACGTTGGTAGT+CGG - chr4.3:37743609-37743628 None:intergenic 45.0%
!! GTTGGCTTTACGATGCAAGT+TGG - chr4.3:37741720-37741739 None:intergenic 45.0%
!! TACTCATCCTCTAAGGCTTG+AGG - chr4.3:37742561-37742580 None:intergenic 45.0%
!! TCTGGCTTTAGCTTGCGATT+TGG + chr4.3:37744517-37744536 MS.gene54591:CDS 45.0%
!! AATAACATATTAATATTATT+TGG - chr4.3:37741621-37741640 None:intergenic 5.0%
!!! ATATTTTAGAAATAATATTT+TGG - chr4.3:37743960-37743979 None:intergenic 5.0%
ACCCAAAACTCTCAGCACAC+TGG + chr4.3:37744578-37744597 MS.gene54591:CDS 50.0%
AGGTGCATCAGCAGCATAAG+AGG - chr4.3:37743689-37743708 None:intergenic 50.0%
CAGCAGCATGAGCAAGAAGT+TGG - chr4.3:37742923-37742942 None:intergenic 50.0%
CGACGCATATCATCGGTACA+AGG + chr4.3:37742094-37742113 MS.gene54591:CDS 50.0%
GAAGGACCCCTTAAGATCCT+CGG - chr4.3:37743638-37743657 None:intergenic 50.0%
GATCGGAAAAGTCGCGAAGA+TGG - chr4.3:37741248-37741267 None:intergenic 50.0%
GATGTTGATGCTGCACAACG+AGG + chr4.3:37743260-37743279 MS.gene54591:CDS 50.0%
GCAGAAGGAAAGCTTAGTGG+AGG + chr4.3:37742354-37742373 MS.gene54591:intron 50.0%
GCATATGGGAAGTTTGCACC+GGG + chr4.3:37742822-37742841 MS.gene54591:CDS 50.0%
GCATTCATGCTCACACTCCA+TGG - chr4.3:37742798-37742817 None:intergenic 50.0%
GCCTCGACTTCATGTTTGGA+TGG + chr4.3:37743282-37743301 MS.gene54591:CDS 50.0%
GCTGTTCCGAAAACAAAGCC+TGG - chr4.3:37741291-37741310 None:intergenic 50.0%
GGGCATAATGAACGGACATG+TGG + chr4.3:37741767-37741786 MS.gene54591:intron 50.0%
GGGCATGAATTCGCTACAGT+TGG - chr4.3:37741738-37741757 None:intergenic 50.0%
GTTCCGAAAACAAAGCCTGG+CGG - chr4.3:37741288-37741307 None:intergenic 50.0%
TAGCCGACGACGCATATCAT+CGG + chr4.3:37742087-37742106 MS.gene54591:CDS 50.0%
TCTTCTTTCGCTTGAGAGGC+CGG - chr4.3:37743242-37743261 None:intergenic 50.0%
TTCCGAAAACAAAGCCTGGC+GGG - chr4.3:37741287-37741306 None:intergenic 50.0%
! AGTGGAGGTGTAAACCGTGA+AGG + chr4.3:37742369-37742388 MS.gene54591:CDS 50.0%
! GTACCGATGATATGCGTCGT+CGG - chr4.3:37742093-37742112 None:intergenic 50.0%
! GTGTCACTCACACAATCCGA+GGG - chr4.3:37742599-37742618 None:intergenic 50.0%
!!! TTCCCGCCAGGCTTTGTTTT+CGG + chr4.3:37741282-37741301 MS.gene54591:intron 50.0%
AACAAAGCCTGGCGGGAAAC+AGG - chr4.3:37741280-37741299 None:intergenic 55.0%
AGGCTTGAGGAACGTCCCAA+TGG - chr4.3:37742548-37742567 None:intergenic 55.0%
CACCGGGTCGATGTTCAGAT+TGG + chr4.3:37742838-37742857 MS.gene54591:CDS 55.0%
CGAGGCCTCGACTTCATGTT+TGG + chr4.3:37743278-37743297 MS.gene54591:CDS 55.0%
GGACGTTCCTCAAGCCTTAG+AGG + chr4.3:37742551-37742570 MS.gene54591:CDS 55.0%
!! GGTGTCACTCACACAATCCG+AGG - chr4.3:37742600-37742619 None:intergenic 55.0%
AATCGGACCTGTTTCCCGCC+AGG + chr4.3:37741270-37741289 MS.gene54591:intron 60.0%
CATCGTGATGGCAAGCCCCT+TGG + chr4.3:37743859-37743878 MS.gene54591:CDS 60.0%
GGTCTGGCATCGCGTAAGTG+AGG - chr4.3:37743709-37743728 None:intergenic 60.0%
TGGCAAGCCCCTTGGTCCAA+TGG + chr4.3:37743867-37743886 MS.gene54591:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 37741220 37744648 37741220 ID=MS.gene54591
chr4.3 mRNA 37741220 37744648 37741220 ID=MS.gene54591.t1;Parent=MS.gene54591
chr4.3 exon 37741220 37741279 37741220 ID=MS.gene54591.t1.exon1;Parent=MS.gene54591.t1
chr4.3 CDS 37741220 37741279 37741220 ID=cds.MS.gene54591.t1;Parent=MS.gene54591.t1
chr4.3 exon 37741456 37741525 37741456 ID=MS.gene54591.t1.exon2;Parent=MS.gene54591.t1
chr4.3 CDS 37741456 37741525 37741456 ID=cds.MS.gene54591.t1;Parent=MS.gene54591.t1
chr4.3 exon 37742057 37742115 37742057 ID=MS.gene54591.t1.exon3;Parent=MS.gene54591.t1
chr4.3 CDS 37742057 37742115 37742057 ID=cds.MS.gene54591.t1;Parent=MS.gene54591.t1
chr4.3 exon 37742197 37742272 37742197 ID=MS.gene54591.t1.exon4;Parent=MS.gene54591.t1
chr4.3 CDS 37742197 37742272 37742197 ID=cds.MS.gene54591.t1;Parent=MS.gene54591.t1
chr4.3 exon 37742358 37742435 37742358 ID=MS.gene54591.t1.exon5;Parent=MS.gene54591.t1
chr4.3 CDS 37742358 37742435 37742358 ID=cds.MS.gene54591.t1;Parent=MS.gene54591.t1
chr4.3 exon 37742520 37742607 37742520 ID=MS.gene54591.t1.exon6;Parent=MS.gene54591.t1
chr4.3 CDS 37742520 37742607 37742520 ID=cds.MS.gene54591.t1;Parent=MS.gene54591.t1
chr4.3 exon 37742716 37742971 37742716 ID=MS.gene54591.t1.exon7;Parent=MS.gene54591.t1
chr4.3 CDS 37742716 37742971 37742716 ID=cds.MS.gene54591.t1;Parent=MS.gene54591.t1
chr4.3 exon 37743188 37743303 37743188 ID=MS.gene54591.t1.exon8;Parent=MS.gene54591.t1
chr4.3 CDS 37743188 37743303 37743188 ID=cds.MS.gene54591.t1;Parent=MS.gene54591.t1
chr4.3 exon 37743546 37743763 37743546 ID=MS.gene54591.t1.exon9;Parent=MS.gene54591.t1
chr4.3 CDS 37743546 37743763 37743546 ID=cds.MS.gene54591.t1;Parent=MS.gene54591.t1
chr4.3 exon 37743857 37743888 37743857 ID=MS.gene54591.t1.exon10;Parent=MS.gene54591.t1
chr4.3 CDS 37743857 37743888 37743857 ID=cds.MS.gene54591.t1;Parent=MS.gene54591.t1
chr4.3 exon 37744010 37744112 37744010 ID=MS.gene54591.t1.exon11;Parent=MS.gene54591.t1
chr4.3 CDS 37744010 37744112 37744010 ID=cds.MS.gene54591.t1;Parent=MS.gene54591.t1
chr4.3 exon 37744209 37744317 37744209 ID=MS.gene54591.t1.exon12;Parent=MS.gene54591.t1
chr4.3 CDS 37744209 37744317 37744209 ID=cds.MS.gene54591.t1;Parent=MS.gene54591.t1
chr4.3 exon 37744456 37744648 37744456 ID=MS.gene54591.t1.exon13;Parent=MS.gene54591.t1
chr4.3 CDS 37744456 37744648 37744456 ID=cds.MS.gene54591.t1;Parent=MS.gene54591.t1
Gene Sequence

>MS.gene54591

ATGCTTTTGAACCTTCTTCACTACTCCATCTTCGCGACTTTTCCGATCTCAATCGGACCTGTTTCCCGCCAGGCTTTGTTTTCGGAACAGCTTCTTCTGCATTTCAGGCATGCACAAACAAACATCTTTCAATTTTATTATTATTATTTATAACATGCATGCAATGTAGAAATGTAGTTATTAGATATTTGATTTTACGTAATGATTAAGTTTCATGTTATTTAAAAATTTTGTAGTACGAAGGTGCAGCATTTGAAGATGGAAAAGGACCAAGTATTTGGGATACTTTCACTCATGAACACCCAGGTTATTTTTAATTACTTCTCTTTCATTTTGCAATTTAAAAAAAATTAATATCATGTTTTTTTTGGTAACATGGGTTCGAACCTGCAACATGTCCAAATAATATTAATATGTTATTTTACATCACGCTTTATTGCACATCTGATCGTAAATCGTTAGATTAAGAGAGGGAAACTCTATCTCAATATAACGGTCCAACTTGCATCGTAAAGCCAACTGTAGCGAATTCATGCCCGTTCATTTAGGGCATAATGAACGGACATGTGGAGCTCCTTTCTTTATGCATGTGGGATCTTGCAAGTTCCATTCAATTTAGTGCAACATTTGCCTTTTCAAAAAAAAAAAATAGTAATAATAATAATTAAACTTAGTGCAACTTGCTGGCTTTTCCTTAATGATGTATTTGGTGCAAGAATCATGATCAAGAGTCATTTTCAGCATAAGTAGCTTCTGAATGCCTTGGAGATCCAACATAGCATAAGGTTTTGCTTGCATGAATGGATTTCTCATATATATTAATGAAATGGCTTGCAGAAAAAATAAGAGATGGAGGTAATGGAGATGTAGCCGACGACGCATATCATCGGTACAAGGTATGTAGTTTTGCAAAGGTATATAAAATACAAATGTAAACATATATTTAGACTCATGATAGATTACTCTATGTATAATAGGAAGATATTGGAATCATAAAGGATTTGAATATGGATGCTTATAGATTTTCCATTGCTTGGTCTAGAGTGCTACCAAGTAAGTCATAGTTTTGTAAATATTCCTCCCTTATACATTGGTGATTTATAGTAACCATCATAAATTTCCTATCAACTATTTGCAGAAGGAAAGCTTAGTGGAGGTGTAAACCGTGAAGGAATAAACTACTACAACAACCTCATCAACGAACTATTGGCTAATGGTAAAGATTCATCAATTTTTATTTACTTATTTTCATATCCATGTGGTTGTCAATCATAAATAATCATTGAGGAGAATAATGCAGGTTTGCAGCCATATGTGACTCTTTTCCATTGGGACGTTCCTCAAGCCTTAGAGGATGAGTATGACGGTTTTTTAAGCCCTCGGATTGTGTGAGTGACACCAATTTTCAATCTAGAGCGATTTGTTTATGACTATTTAGGGAAATATTGCAGTAAATAAAATTAGTCGATTAACATATGTAATCCGCGATCTTGCAGAGACGATTTTAGGGACTATGCTGACATTTGCTACAAGGAATTTGGTGATAGAGTGAAACATTGGATTACTTTGAATGAACCATGGAGTGTGAGCATGAATGCTTATGCATATGGGAAGTTTGCACCGGGTCGATGTTCAGATTGGTTAAATCTGAATTGCACAGGAGGTGATTCGGGAACAGAACCATATTTGGCTGCACACTACCAACTTCTTGCTCATGCTGCTGCTACAAAACTATACAAGACCAAATATCAGGTCTTCTGTTTTTAAAAGCAATATATTATTCAAATTCGGCACTGAATCTTTATGTCCTGAAATTCAAATAACACGAGACTTGATCTTCTGTAATAGAAGGGCAAGTCGGACAAAAGTATACACAATTGCTTTCTATTGATAGATCATACTTATTTTTTTAGTGTGAACTTATTAGAGCTGTTTCTAACCAAATAACCAAAATTTTGAAATATGCAGGCATCTCAATATGGTATAATAGGGATTACCTTACTCTCTCATTGGTATGAGCCGGCCTCTCAAGCGAAAGAAGATGTTGATGCTGCACAACGAGGCCTCGACTTCATGTTTGGATGGTAAAGATTAAACTTAACTATTCGTAGCTCAACACTAACAGATATGATCATGATAATCTGGTTAAAATTGAATGTAAACTTCATTTGATTTTGGTTCAATCTGTGTTTTGAACTCAAGAAATAAGTTATGTAGATTTGCTGAATTCAAATGCAACTATATGCCATTTGTGCAAAGTAATATGAAATCAAAGAAGACTAATACATTTTTTTATTGCATCTGCATTTTGCATTTTTTTCTATAGGTATATGGATCCAATCACAAAAGGAAACTATCCAAAAAGCATGCAATCTTTGGTTGGAAGCCGACTACCAACGTTCTCCAAAGAAGAATCCGAGGATCTTAAGGGGTCCTTCGATTTTCTAGGCCTAAACTATTACTCATCCTCTTATGCTGCTGATGCACCTCACTTACGCGATGCCAGACCCACCATACAAACTGATTCTCTTATCAATGCTACATGTAATTATCAAATTAAATATAAGCTTGCACTAGTTAATTATACATACTCTAAACTTTATATCTAATCATGCTTTATTATTTTTTCGATGACAGTTCATCGTGATGGCAAGCCCCTTGGTCCAATGGTATATATTTTATCATTCATTAGCATCTAATTTGCATATCAATGCCTCTCATTTGTAGCTTTTGTGTTCCAAAATATTATTTCTAAAATATAACGATAACCTTTTTTTTAATTATATGCAGTCTGCTTCAAGTTGGTTATGCATTTATCCGCGAGGATTAGGACAACTGTTGCTTTACGTCAAGAATAAGTATAACAACCCCGTAATTTACATTACCGAAAATGGTAATAACTCTATTATACTTCTTATTCTCTAAGATATTATATGCATAATACTGAAACTTTAATAAAGCTAATGAAAGTGACAAAATCAATTGATAGGTCGCGATGAGTTCAATGATCCAAAACTGTCGCTTGAAGAATCCCTTTTAGATACTTACAGAATCGATTACTTTTACCGTCATCTTTATTATGTTCAAGCTGCAATCAGGTAACTAATGCTTTATCAGAAAAACCTCTAATTAAGTTTTAGCTACTGATTTATAGGTTTTATACATATAAATTCACAATGCATCTTTTCTACCCTTCATTTTTACATTTACCTATTATTGACCTTTATGAATAACAGGGAAGGTGTGAATGTAAAGGGATATTTTGCATGGTCATTGTTGGATAACCATGAATGGGAATCTGGCTTTAGCTTGCGATTTGGACTCGTATTTGTGGATTACAACGACGACTTGAAAAGACACCCAAAACTCTCAGCACACTGGTTCAAGAATTTCCTCAAAAAATCTTGCCAAGAAAAGTGTTCTCTTTAA
Protein sequence

>MS.gene54591.t1

MLLNLLHYSIFATFPISIGPYEGAAFEDGKGPSIWDTFTHEHPEKIRDGGNGDVADDAYHRYKEDIGIIKDLNMDAYRFSIAWSRVLPKGKLSGGVNREGINYYNNLINELLANGLQPYVTLFHWDVPQALEDEYDGFLSPRIVDDFRDYADICYKEFGDRVKHWITLNEPWSVSMNAYAYGKFAPGRCSDWLNLNCTGGDSGTEPYLAAHYQLLAHAAATKLYKTKYQASQYGIIGITLLSHWYEPASQAKEDVDAAQRGLDFMFGWYMDPITKGNYPKSMQSLVGSRLPTFSKEESEDLKGSFDFLGLNYYSSSYAADAPHLRDARPTIQTDSLINATFHRDGKPLGPMSASSWLCIYPRGLGQLLLYVKNKYNNPVIYITENGRDEFNDPKLSLEESLLDTYRIDYFYRHLYYVQAAIREGVNVKGYFAWSLLDNHEWESGFSLRFGLVFVDYNDDLKRHPKLSAHWFKNFLKKSCQEKCSL