AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056347

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056347.t1 MTR_4g066210 97.849 465 10 0 21 485 63 527 0.0 953
MS.gene056347.t1 MTR_4g066240 88.646 458 52 0 21 478 56 513 0.0 850
MS.gene056347.t1 MTR_4g066240 88.453 459 52 1 21 478 56 514 0.0 847
MS.gene056347.t1 MTR_4g066340 79.080 435 91 0 44 478 6 440 0.0 732
MS.gene056347.t1 MTR_4g066580 69.348 460 141 0 21 480 59 518 0.0 700
MS.gene056347.t1 MTR_4g066630 69.451 455 139 0 21 475 59 513 0.0 691
MS.gene056347.t1 MTR_3g028720 67.826 460 148 0 21 480 58 517 0.0 690
MS.gene056347.t1 MTR_2g098790 69.365 457 140 0 21 477 58 514 0.0 689
MS.gene056347.t1 MTR_4g066590 67.462 461 150 0 21 481 53 513 0.0 684
MS.gene056347.t1 MTR_2g098520 67.615 457 148 0 21 477 54 510 0.0 682
MS.gene056347.t1 MTR_4g066240 88.011 367 44 0 21 387 56 422 0.0 681
MS.gene056347.t1 MTR_2g098540 67.396 457 149 0 21 477 51 507 0.0 678
MS.gene056347.t1 MTR_6g078450 66.667 459 153 0 21 479 56 514 0.0 677
MS.gene056347.t1 MTR_2g098540 66.883 462 153 0 16 477 2 463 0.0 677
MS.gene056347.t1 MTR_6g078330 67.396 457 149 0 21 477 57 513 0.0 668
MS.gene056347.t1 MTR_6g078380 69.147 457 140 1 21 476 56 512 0.0 660
MS.gene056347.t1 MTR_4g066640 64.052 459 161 2 21 478 59 514 0.0 643
MS.gene056347.t1 MTR_5g071520 64.163 466 158 1 21 477 59 524 0.0 618
MS.gene056347.t1 MTR_4g066330 63.181 459 141 2 21 477 61 493 0.0 610
MS.gene056347.t1 MTR_4g066630 69.327 401 123 0 21 421 59 459 0.0 609
MS.gene056347.t1 MTR_3g028720 68.159 402 128 0 21 422 58 459 0.0 607
MS.gene056347.t1 MTR_6g078330 67.482 409 133 0 69 477 1 409 0.0 600
MS.gene056347.t1 MTR_4g066280 61.656 459 148 2 21 477 61 493 0.0 600
MS.gene056347.t1 MTR_6g078450 67.910 402 129 0 21 422 56 457 0.0 599
MS.gene056347.t1 MTR_3g026280 59.737 457 179 2 21 477 42 493 0.0 584
MS.gene056347.t1 MTR_6g078330 65.995 397 133 1 81 477 15 409 0.0 565
MS.gene056347.t1 MTR_4g066580 69.189 370 114 0 21 390 59 428 0.0 565
MS.gene056347.t1 MTR_4g066630 69.482 367 112 0 21 387 59 425 0.0 563
MS.gene056347.t1 MTR_3g028720 68.478 368 116 0 21 388 58 425 0.0 558
MS.gene056347.t1 MTR_4g023000 57.987 457 186 3 21 477 44 494 0.0 555
MS.gene056347.t1 MTR_4g067040 68.079 354 113 0 21 374 54 407 0.0 530
MS.gene056347.t1 MTR_2g043200 57.883 463 161 7 21 477 85 519 0.0 528
MS.gene056347.t1 MTR_4g015420 54.158 469 175 6 21 477 85 525 0.0 525
MS.gene056347.t1 MTR_3g026400 53.173 457 208 4 22 477 27 478 4.02e-180 513
MS.gene056347.t1 MTR_4g067040 69.909 329 99 0 21 349 54 382 6.27e-180 509
MS.gene056347.t1 MTR_3g026400 53.173 457 208 4 22 477 58 509 6.83e-180 514
MS.gene056347.t1 MTR_3g028720 68.997 329 102 0 21 349 58 386 3.52e-179 508
MS.gene056347.t1 MTR_4g066630 70.755 318 93 0 21 338 59 376 9.26e-176 498
MS.gene056347.t1 MTR_4g083000 52.411 477 217 6 4 476 32 502 1.84e-174 500
MS.gene056347.t1 MTR_4g015460 53.533 467 178 9 21 476 62 500 1.20e-173 498
MS.gene056347.t1 MTR_3g026620 53.289 456 209 3 22 477 59 510 4.10e-173 497
MS.gene056347.t1 MTR_5g044000 52.838 458 210 4 21 475 66 520 1.51e-170 491
MS.gene056347.t1 MTR_6g078330 67.500 320 104 0 21 340 57 376 1.62e-164 470
MS.gene056347.t1 MTR_4g066640 62.840 331 119 2 21 350 59 386 3.20e-159 457
MS.gene056347.t1 MTR_4g081530 48.643 479 237 6 10 484 34 507 3.68e-156 454
MS.gene056347.t1 MTR_0110s0040 49.234 457 223 6 22 477 64 512 6.02e-154 448
MS.gene056347.t1 MTR_0110s0030 49.015 457 224 6 22 477 64 512 7.17e-154 448
MS.gene056347.t1 MTR_4g131810 48.908 458 229 5 21 475 48 503 8.73e-154 448
MS.gene056347.t1 MTR_4g083000 52.184 412 189 4 69 476 1 408 4.53e-152 439
MS.gene056347.t1 MTR_2g022760 48.380 463 234 5 21 481 56 515 2.63e-149 436
MS.gene056347.t1 MTR_3g033790 46.414 474 234 7 13 482 29 486 4.10e-147 430
MS.gene056347.t1 MTR_3g033810 45.975 472 240 5 13 482 31 489 9.09e-145 424
MS.gene056347.t1 MTR_4g131800 47.948 463 235 6 21 481 536 994 5.20e-143 436
MS.gene056347.t1 MTR_2g079550 45.474 464 242 6 22 481 51 507 9.34e-141 415
MS.gene056347.t1 MTR_2g079580 45.043 464 244 6 22 481 53 509 2.17e-140 414
MS.gene056347.t1 MTR_2g022710 43.791 459 252 5 21 477 55 509 1.87e-138 409
MS.gene056347.t1 MTR_7g068910 44.161 471 241 7 13 477 41 495 2.85e-138 408
MS.gene056347.t1 MTR_7g033860 46.087 460 233 5 22 479 69 515 6.89e-138 407
MS.gene056347.t1 MTR_7g068910 43.243 481 241 8 13 477 41 505 4.26e-135 400
MS.gene056347.t1 MTR_3g026400 61.279 297 112 2 22 318 58 351 4.31e-133 390
MS.gene056347.t1 MTR_4g016920 43.226 465 246 7 22 483 48 497 1.68e-130 388
MS.gene056347.t1 MTR_5g068740 43.601 461 239 8 22 478 48 491 2.11e-129 385
MS.gene056347.t1 MTR_0108s0030 43.043 460 233 4 22 481 24 454 5.01e-128 380
MS.gene056347.t1 MTR_2g022760 46.747 415 216 5 69 481 1 412 2.77e-126 374
MS.gene056347.t1 MTR_1g088610 43.764 457 239 7 22 476 48 488 1.04e-123 375
MS.gene056347.t1 MTR_1g088610 40.336 119 64 2 361 478 503 615 2.27e-17 85.5
MS.gene056347.t1 MTR_7g068900 44.836 426 199 8 64 476 17 419 7.95e-120 360
MS.gene056347.t1 MTR_2g022710 42.857 406 227 4 74 477 6 408 1.15e-119 357
MS.gene056347.t1 MTR_1g088630 41.438 473 231 10 10 478 4 434 1.02e-117 353
MS.gene056347.t1 MTR_7g051510 43.966 464 234 8 22 481 96 537 2.23e-117 355
MS.gene056347.t1 MTR_7g051610 44.035 461 236 6 17 475 91 531 7.43e-116 351
MS.gene056347.t1 MTR_2g079580 46.883 369 186 5 22 386 53 415 8.66e-116 347
MS.gene056347.t1 MTR_2g022710 42.327 404 228 4 21 422 55 455 4.65e-115 347
MS.gene056347.t1 MTR_2g022710 42.327 404 228 4 21 422 55 455 9.00e-115 347
MS.gene056347.t1 MTR_7g051650 42.325 456 242 5 22 475 96 532 3.19e-114 347
MS.gene056347.t1 MTR_4g016920 42.105 418 225 6 69 483 1 404 2.15e-113 341
MS.gene056347.t1 MTR_7g051650 41.886 456 240 5 22 475 96 528 5.74e-113 344
MS.gene056347.t1 MTR_2g022710 43.902 369 202 4 21 387 55 420 1.11e-112 339
MS.gene056347.t1 MTR_0110s0040 51.438 313 148 3 22 334 64 372 3.38e-110 332
MS.gene056347.t1 MTR_2g022710 43.258 356 197 4 21 374 55 407 7.59e-106 322
MS.gene056347.t1 MTR_7g056747 43.510 416 205 5 63 475 9 397 8.34e-106 321
MS.gene056347.t1 MTR_2g022710 48.173 301 153 3 21 321 55 352 4.54e-99 305
MS.gene056347.t1 MTR_1g088630 47.000 300 129 5 22 320 48 318 8.81e-92 284
MS.gene056347.t1 MTR_4g016920 50.794 252 122 1 69 320 1 250 6.29e-91 280
MS.gene056347.t1 MTR_1g088630 50.202 247 117 3 22 267 48 289 1.43e-81 255
MS.gene056347.t1 MTR_0110s0020 49.784 231 110 4 13 241 53 279 3.24e-74 236
MS.gene056347.t1 MTR_4g122980 27.512 418 228 14 65 475 183 532 1.99e-29 122
MS.gene056347.t1 MTR_5g069350 40.782 179 53 5 315 477 48 189 7.11e-29 112
MS.gene056347.t1 MTR_8g038650 45.000 100 55 0 227 326 57 156 5.61e-24 98.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056347.t1 AT2G44450 54.267 457 202 3 22 477 52 502 0.0 536
MS.gene056347.t1 AT5G44640 54.267 457 203 3 22 477 52 503 0.0 534
MS.gene056347.t1 AT5G42260 54.989 451 197 3 22 471 52 497 0.0 532
MS.gene056347.t1 AT2G44480 52.954 457 214 1 22 478 87 542 0.0 530
MS.gene056347.t1 AT2G44480 52.954 457 214 1 22 478 56 511 0.0 530
MS.gene056347.t1 AT3G60130 54.605 456 201 3 22 476 51 501 0.0 518
MS.gene056347.t1 AT2G25630 53.187 455 190 4 22 475 51 483 9.09e-176 503
MS.gene056347.t1 AT5G24550 52.165 462 216 5 21 478 52 512 1.01e-174 502
MS.gene056347.t1 AT5G24540 52.165 462 216 5 21 478 52 512 3.39e-166 480
MS.gene056347.t1 AT3G60140 48.837 473 237 5 10 479 36 506 2.96e-165 479
MS.gene056347.t1 AT1G26560 49.247 465 222 5 21 478 52 509 6.07e-164 473
MS.gene056347.t1 AT1G51470 50.437 458 213 6 22 477 66 511 3.12e-162 469
MS.gene056347.t1 AT5G54570 50.980 459 218 4 21 475 50 505 4.63e-162 469
MS.gene056347.t1 AT2G44480 51.463 410 198 1 69 478 1 409 1.30e-161 464
MS.gene056347.t1 AT2G44460 51.183 465 220 6 21 481 49 510 1.26e-160 468
MS.gene056347.t1 AT2G44470 47.789 475 235 6 10 477 39 507 1.83e-160 468
MS.gene056347.t1 AT1G47600 50.000 462 209 7 22 477 66 511 3.40e-160 464
MS.gene056347.t1 AT3G60130 53.253 415 188 3 63 476 40 449 3.80e-159 459
MS.gene056347.t1 AT1G47600 49.784 462 209 7 22 477 66 510 3.97e-159 461
MS.gene056347.t1 AT3G60120 49.676 463 222 7 21 477 34 491 1.53e-157 458
MS.gene056347.t1 AT2G44490 48.085 470 238 5 21 485 34 502 1.58e-155 454
MS.gene056347.t1 AT3G18080 49.237 459 223 7 22 478 60 510 4.59e-155 451
MS.gene056347.t1 AT5G25980 48.380 463 222 10 29 482 75 529 3.08e-151 442
MS.gene056347.t1 AT5G36890 48.626 473 228 5 13 482 26 486 3.08e-150 437
MS.gene056347.t1 AT5G36890 48.626 473 228 5 13 482 26 486 3.96e-150 437
MS.gene056347.t1 AT3G60130 51.807 415 183 4 63 476 40 438 4.20e-150 436
MS.gene056347.t1 AT5G26000 48.478 460 213 9 29 475 63 511 6.18e-148 434
MS.gene056347.t1 AT2G32860 45.397 478 256 5 10 483 104 580 1.05e-147 436
MS.gene056347.t1 AT3G18070 45.969 459 228 9 22 477 51 492 3.73e-147 430
MS.gene056347.t1 AT2G32860 45.397 478 255 6 10 483 104 579 2.20e-146 432
MS.gene056347.t1 AT3G18070 45.969 459 234 9 22 477 51 498 5.47e-146 427
MS.gene056347.t1 AT1G61820 47.186 462 238 5 21 479 50 508 2.03e-144 424
MS.gene056347.t1 AT3G03640 45.455 462 246 4 21 477 54 514 2.65e-144 424
MS.gene056347.t1 AT5G54570 50.608 411 196 4 69 475 1 408 1.71e-143 419
MS.gene056347.t1 AT1G02850 46.667 465 221 8 22 480 47 490 3.78e-141 415
MS.gene056347.t1 AT3G09260 45.455 462 247 5 22 480 56 515 6.69e-140 412
MS.gene056347.t1 AT1G75940 46.104 462 239 7 22 475 58 517 1.02e-139 412
MS.gene056347.t1 AT5G54570 50.370 405 195 3 21 422 50 451 1.90e-138 406
MS.gene056347.t1 AT5G24540 50.495 404 196 4 21 421 52 454 2.49e-138 406
MS.gene056347.t1 AT2G44470 47.129 418 209 5 10 421 39 450 3.45e-137 403
MS.gene056347.t1 AT1G02850 44.856 486 218 9 22 478 47 511 2.10e-136 404
MS.gene056347.t1 AT3G21370 45.064 466 238 9 22 476 54 512 3.50e-136 403
MS.gene056347.t1 AT5G28510 43.897 467 252 6 22 480 60 524 5.67e-136 403
MS.gene056347.t1 AT2G44450 52.661 357 162 3 122 477 1 351 3.31e-135 394
MS.gene056347.t1 AT1G66280 45.690 464 242 7 22 480 57 515 4.03e-135 400
MS.gene056347.t1 AT1G02850 44.764 487 218 10 22 478 47 512 1.17e-134 399
MS.gene056347.t1 AT3G60140 48.564 383 193 4 10 390 36 416 1.14e-133 394
MS.gene056347.t1 AT1G52400 45.788 463 237 10 22 476 60 516 1.74e-133 396
MS.gene056347.t1 AT1G52400 45.788 463 237 10 22 476 60 516 1.74e-133 396
MS.gene056347.t1 AT4G21760 43.326 457 248 6 21 475 74 521 4.18e-133 396
MS.gene056347.t1 AT5G25980 49.127 401 189 8 29 422 75 467 7.51e-133 392
MS.gene056347.t1 AT5G25980 49.127 401 189 8 29 422 75 467 7.51e-133 392
MS.gene056347.t1 AT1G66270 44.060 463 251 6 22 480 57 515 1.41e-132 394
MS.gene056347.t1 AT4G27830 42.117 463 247 7 21 479 43 488 2.91e-132 392
MS.gene056347.t1 AT1G61810 43.011 465 259 5 21 482 53 514 9.46e-132 392
MS.gene056347.t1 AT1G02850 45.376 465 200 9 22 480 47 463 2.87e-131 389
MS.gene056347.t1 AT1G66270 43.844 463 250 7 22 480 57 513 1.80e-129 386
MS.gene056347.t1 AT5G26000 47.772 404 191 6 29 422 63 456 7.36e-129 382
MS.gene056347.t1 AT4G22100 43.290 462 236 9 21 476 42 483 3.62e-127 379
MS.gene056347.t1 AT1G60090 42.299 461 240 9 21 476 44 483 6.66e-127 379
MS.gene056347.t1 AT4G21760 42.670 457 241 7 21 475 74 511 2.09e-126 379
MS.gene056347.t1 AT2G44480 56.897 290 123 2 22 311 87 374 3.93e-125 370
MS.gene056347.t1 AT1G61810 43.371 445 246 5 21 462 53 494 6.40e-125 375
MS.gene056347.t1 AT4G27820 41.901 463 246 7 21 479 43 486 7.31e-124 371
MS.gene056347.t1 AT1G60090 41.595 464 246 10 21 476 44 490 3.71e-123 369
MS.gene056347.t1 AT1G45191 40.909 462 251 8 16 476 44 484 5.97e-120 361
MS.gene056347.t1 AT1G45191 40.430 465 255 8 16 476 44 490 4.08e-118 356
MS.gene056347.t1 AT3G62750 40.909 462 241 9 21 478 43 476 1.41e-117 355
MS.gene056347.t1 AT1G45191 39.915 471 254 8 16 476 44 495 1.41e-117 355
MS.gene056347.t1 AT3G62750 40.821 463 242 9 21 479 69 503 1.91e-117 355
MS.gene056347.t1 AT2G44470 48.538 342 168 4 10 345 39 378 3.17e-117 350
MS.gene056347.t1 AT5G48375 41.403 442 201 6 29 468 54 439 1.57e-116 350
MS.gene056347.t1 AT1G45191 39.331 478 259 9 16 476 44 507 5.54e-115 349
MS.gene056347.t1 AT1G61810 42.271 414 233 5 72 482 4 414 4.23e-114 343
MS.gene056347.t1 AT2G44460 49.180 366 180 5 120 481 1 364 5.08e-114 343
MS.gene056347.t1 AT4G27830 41.687 415 223 7 69 479 1 400 6.90e-113 340
MS.gene056347.t1 AT1G02850 44.784 393 174 6 22 386 47 424 9.96e-110 332
MS.gene056347.t1 AT3G62740 37.708 480 258 11 21 484 43 497 1.02e-109 335
MS.gene056347.t1 AT1G52400 45.209 407 210 9 22 421 60 460 1.10e-109 333
MS.gene056347.t1 AT4G27820 40.723 415 228 6 69 479 1 401 1.71e-104 318
MS.gene056347.t1 AT4G27820 40.723 415 228 6 69 479 1 401 1.71e-104 318
MS.gene056347.t1 AT1G61820 44.474 371 201 4 112 479 1 369 2.56e-104 316
MS.gene056347.t1 AT2G44480 48.173 301 155 1 178 478 6 305 3.11e-101 306
MS.gene056347.t1 AT1G61810 42.005 369 209 4 21 386 53 419 2.07e-100 308
MS.gene056347.t1 AT3G62750 40.786 407 210 8 21 424 69 447 4.53e-100 308
MS.gene056347.t1 AT3G18070 45.593 329 170 6 152 477 99 421 3.64e-99 305
MS.gene056347.t1 AT3G62750 39.518 415 224 8 69 479 1 392 5.30e-99 304
MS.gene056347.t1 AT3G62750 39.518 415 224 8 69 479 1 392 5.30e-99 304
MS.gene056347.t1 AT1G60270 48.464 293 144 3 21 313 44 329 7.89e-99 302
MS.gene056347.t1 AT3G18070 47.492 299 151 4 22 320 51 343 1.53e-98 302
MS.gene056347.t1 AT1G51490 46.647 343 162 8 13 349 30 357 1.11e-97 300
MS.gene056347.t1 AT3G62740 38.424 406 220 9 21 421 43 423 6.93e-94 291
MS.gene056347.t1 AT3G18070 45.397 315 163 6 166 477 1 309 1.45e-93 286
MS.gene056347.t1 AT3G62750 44.224 303 151 5 21 322 43 328 2.98e-91 283
MS.gene056347.t1 AT3G62750 44.224 303 151 5 21 322 43 328 2.98e-91 283
MS.gene056347.t1 AT1G45191 43.934 305 163 3 16 320 44 340 3.24e-91 284
MS.gene056347.t1 AT1G45191 43.934 305 163 3 16 320 44 340 5.25e-91 283
MS.gene056347.t1 AT3G62750 44.224 303 151 5 21 322 69 354 1.34e-90 283
MS.gene056347.t1 AT1G60270 48.560 243 122 2 71 313 3 242 3.38e-80 251
MS.gene056347.t1 AT1G61810 47.600 250 129 2 21 270 53 300 2.74e-79 249
MS.gene056347.t1 AT3G18070 43.220 236 130 3 153 386 100 333 2.27e-65 215
MS.gene056347.t1 AT3G18070 42.601 223 124 3 166 386 1 221 7.35e-60 196
MS.gene056347.t1 AT5G16580 37.282 287 165 7 147 432 27 299 3.63e-51 176
MS.gene056347.t1 AT3G06510 25.776 419 238 13 64 476 159 510 9.72e-31 126
MS.gene056347.t1 AT3G06510 25.455 440 247 13 64 476 159 544 4.23e-30 124

Find 112 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGAAAAGGACCAAGTATT+TGG 0.197865 4.1:+34656965 MS.gene056347:CDS
GCATCTCAATATGGTATAAT+AGG 0.209400 4.1:+34658705 MS.gene056347:CDS
GAACTTTGCTACAAGGAATT+TGG 0.231439 4.1:+34658255 MS.gene056347:CDS
AAAACAGAAGACCTGATATT+TGG 0.235314 4.1:-34658478 None:intergenic
GTAATTTACATTACCGAAAA+TGG 0.241625 4.1:+34659607 MS.gene056347:CDS
CGATTCTGTAAGTATCTAAA+AGG 0.249841 4.1:-34659768 None:intergenic
TTCGGGAACAGAACCATATT+TGG 0.289048 4.1:+34658404 MS.gene056347:CDS
ACTCGTCTTTGTGGATTATA+AGG 0.289629 4.1:+34660053 MS.gene056347:CDS
AATCATAAAGGATTTGAATA+TGG 0.307459 4.1:+34657730 MS.gene056347:CDS
AAGATCCTCGGATTCTTCTT+TGG 0.311879 4.1:-34659140 None:intergenic
CAACCTCATCGACGAAATAT+TGG 0.323590 4.1:+34657929 MS.gene056347:CDS
AACCAATGTGCCGAGAGTTT+TGG 0.337227 4.1:-34660093 None:intergenic
TGGTAGTGTGCAGCCAAATA+TGG 0.343520 4.1:-34658417 None:intergenic
CGAGGCCTCGACTTCATGTT+TGG 0.348528 4.1:+34658795 MS.gene056347:CDS
AGAAGAATCCGAGGATCTTA+AGG 0.358352 4.1:+34659144 MS.gene056347:CDS
GATTCTTCGAGTGACAGTTT+TGG 0.360269 4.1:-34659745 None:intergenic
GGACGTTCCTCAAGCCTTAG+AGG 0.360927 4.1:+34658068 MS.gene056347:CDS
TGCATTTATCCGCGAGGATT+AGG 0.361716 4.1:+34659544 MS.gene056347:CDS
TCTGGCTTCAGCTTGCGATT+TGG 0.362792 4.1:+34660030 MS.gene056347:CDS
TATGTATTTCAGGAAGATAT+TGG 0.369128 4.1:+34657707 MS.gene056347:intron
GCTACAAGGAATTTGGTGAT+AGG 0.371977 4.1:+34658262 MS.gene056347:CDS
TGAAGCCAGATTCCCATTCA+TGG 0.380755 4.1:-34660017 None:intergenic
AAAATAAGAGATGGAGGTAA+TGG 0.386283 4.1:+34657589 MS.gene056347:CDS
GGAAGATATTGGAATCATAA+AGG 0.386661 4.1:+34657718 MS.gene056347:intron
AATTGCACAGGAGGTGATTC+GGG 0.389673 4.1:+34658387 MS.gene056347:CDS
GATAAGAGAATCAGTTTGTA+TGG 0.392110 4.1:-34659248 None:intergenic
TGGAAAAGGACCAAGTATTT+GGG 0.404129 4.1:+34656966 MS.gene056347:CDS
GATAACCATGAATGGGAATC+TGG 0.405714 4.1:+34660012 MS.gene056347:CDS
GAATTGCACAGGAGGTGATT+CGG 0.408321 4.1:+34658386 MS.gene056347:CDS
CATCTCAATATGGTATAATA+GGG 0.411825 4.1:+34658706 MS.gene056347:CDS
AGGTGCATCAGCAGCATAAG+AGG 0.412498 4.1:-34659203 None:intergenic
TATAGTAGTTTATTCCTTCA+CGG 0.415321 4.1:-34657905 None:intergenic
AATATGCAGGCATCTCAATA+TGG 0.419382 4.1:+34658696 MS.gene056347:intron
ATCAGTTTGTATGGTGGGTC+TGG 0.422909 4.1:-34659239 None:intergenic
AGGGAAGGTGTGAATGTAAA+GGG 0.435489 4.1:+34659967 MS.gene056347:intron
GCGATTTGGACTCGTCTTTG+TGG 0.435971 4.1:+34660044 MS.gene056347:CDS
ATTATGTTCAAGCTGCAATC+AGG 0.440754 4.1:+34659812 MS.gene056347:CDS
TTTGCAGAAGGAAAGCTTAG+TGG 0.446993 4.1:+34657873 MS.gene056347:intron
ACTATACAAGACCAAATATC+AGG 0.449280 4.1:+34658467 MS.gene056347:CDS
TCTTCTTTCGCTTGAGAGGC+CGG 0.450631 4.1:-34658756 None:intergenic
AGCATGAATGCTTATGCATA+TGG 0.456434 4.1:+34658324 MS.gene056347:CDS
GGATTACCTTACTCTCTCAT+TGG 0.457583 4.1:+34658727 MS.gene056347:CDS
CTTTGGAGAACTTTGGTAGC+CGG 0.461441 4.1:-34659123 None:intergenic
GAAGAATCCGAGGATCTTAA+GGG 0.463005 4.1:+34659145 MS.gene056347:CDS
AAGAGGATGAGTAATAGTTT+AGG 0.463613 4.1:-34659186 None:intergenic
CACCGGGTCGATGTTCAGAT+TGG 0.463655 4.1:+34658355 MS.gene056347:CDS
CTACAAGGAATTTGGTGATA+GGG 0.466831 4.1:+34658263 MS.gene056347:CDS
ACCGTCATACTCATCCTCTA+AGG 0.470920 4.1:-34658082 None:intergenic
GATTCTTCTTTGGAGAACTT+TGG 0.475194 4.1:-34659130 None:intergenic
TGCATATGGGAAGTTTGCAC+CGG 0.482317 4.1:+34658338 MS.gene056347:CDS
TGCGATCTTTGGTTGGAAGC+CGG 0.484554 4.1:+34659104 MS.gene056347:CDS
CATCGTGATGGCAAGCCCCT+TGG 0.493086 4.1:+34659376 MS.gene056347:CDS
CAGGGAAGGTGTGAATGTAA+AGG 0.498317 4.1:+34659966 MS.gene056347:intron
CTCATACCAATGAGAGAGTA+AGG 0.499326 4.1:-34658733 None:intergenic
AACATCTTCTTTCGCTTGAG+AGG 0.502079 4.1:-34658760 None:intergenic
CAGCAGCATGAGCAAGAAGT+TGG 0.509755 4.1:-34658437 None:intergenic
TATATGGATCCAATCACAAA+AGG 0.510867 4.1:+34659064 MS.gene056347:CDS
CATTGTTGGATAACCATGAA+TGG 0.514197 4.1:+34660004 MS.gene056347:CDS
ATCGACGAAATATTGGCAAA+TGG 0.514581 4.1:+34657936 MS.gene056347:CDS
CAATGGAAAAGTGTCACATA+TGG 0.522829 4.1:-34658045 None:intergenic
ATTGTTGGATAACCATGAAT+GGG 0.522990 4.1:+34660005 MS.gene056347:CDS
TGGTTAAATCTGAATTGCAC+AGG 0.528273 4.1:+34658375 MS.gene056347:CDS
TTGGTGATAGGGTAAAACAT+TGG 0.531541 4.1:+34658274 MS.gene056347:CDS
ACTCTCTCATTGGTATGAGC+CGG 0.532738 4.1:+34658737 MS.gene056347:CDS
TGGCAAGCCCCTTGGTCCAA+TGG 0.533454 4.1:+34659384 MS.gene056347:CDS
GCAGCATTTGAAGATGGAAA+AGG 0.538475 4.1:+34656952 MS.gene056347:CDS
AGAGAATCAGTTTGTATGGT+GGG 0.539142 4.1:-34659244 None:intergenic
GATTCTGTAAGTATCTAAAA+GGG 0.541771 4.1:-34659767 None:intergenic
AATATATACCATTGGACCAA+GGG 0.544956 4.1:-34659392 None:intergenic
CTATGCTGAACTTTGCTACA+AGG 0.545879 4.1:+34658248 MS.gene056347:CDS
AAAAGCATGCGATCTTTGGT+TGG 0.548161 4.1:+34659097 MS.gene056347:CDS
TCGATGACAGTTCATCGTGA+TGG 0.548253 4.1:+34659364 MS.gene056347:intron
GCCTCGACTTCATGTTTGGA+TGG 0.549073 4.1:+34658799 MS.gene056347:CDS
GAAGGTGCAGCATTTGAAGA+TGG 0.552430 4.1:+34656946 MS.gene056347:CDS
GTGAAAGTATCCCAAATACT+TGG 0.559968 4.1:-34656976 None:intergenic
CGAAGATTGAGTAGTGAAGA+AGG 0.564432 4.1:-34656719 None:intergenic
ATATGCAGTCTGCTTCAAGT+TGG 0.568943 4.1:+34659518 MS.gene056347:intron
CGACGCATATCATCGGTACA+AGG 0.572001 4.1:+34657624 MS.gene056347:CDS
GCATTCATGCTCACACTCCA+TGG 0.576199 4.1:-34658312 None:intergenic
GAAACAGGTCCGATTGAGAT+CGG 0.577440 4.1:-34656750 None:intergenic
GAAACGACACCCAAAACTCT+CGG 0.590271 4.1:+34660083 MS.gene056347:CDS
TACTCATCCTCTAAGGCTTG+AGG 0.594134 4.1:-34658075 None:intergenic
ACCCAAAACTCTCGGCACAT+TGG 0.595661 4.1:+34660091 MS.gene056347:CDS
TAGCCGACGACGCATATCAT+CGG 0.598194 4.1:+34657617 MS.gene056347:CDS
AAATATATACCATTGGACCA+AGG 0.598574 4.1:-34659393 None:intergenic
GGATTACTTTGAATGAACCA+TGG 0.603853 4.1:+34658295 MS.gene056347:CDS
TTGCCAATATTTCGTCGATG+AGG 0.603988 4.1:-34657932 None:intergenic
AGGCTTGAGGAACGTCCCAA+TGG 0.604842 4.1:-34658062 None:intergenic
GTTTAGGCCTAGAAAATCGA+AGG 0.606609 4.1:-34659170 None:intergenic
GCATGAATGCTTATGCATAT+GGG 0.610049 4.1:+34658325 MS.gene056347:CDS
GAAGGACCCCTTAAGATCCT+CGG 0.611639 4.1:-34659152 None:intergenic
AACCAATCTGAACATCGACC+CGG 0.611784 4.1:-34658357 None:intergenic
GGTGTCACTCACACAATCCG+AGG 0.616673 4.1:-34658114 None:intergenic
GGTCTGGCATCGCGTAAGTG+AGG 0.619875 4.1:-34659223 None:intergenic
GCATATGGGAAGTTTGCACC+GGG 0.626791 4.1:+34658339 MS.gene056347:CDS
AAGAGAATCAGTTTGTATGG+TGG 0.627183 4.1:-34659245 None:intergenic
GCCTTAGAGGATGAGTATGA+CGG 0.628770 4.1:+34658081 MS.gene056347:CDS
GCAGAAGGAAAGCTTAGTGG+AGG 0.637778 4.1:+34657876 MS.gene056347:intron
ATATATACCATTGGACCAAG+GGG 0.639799 4.1:-34659391 None:intergenic
AGTGGAGGTGTAAACCGTGA+AGG 0.641058 4.1:+34657891 MS.gene056347:CDS
ACCATCCAAACATGAAGTCG+AGG 0.641696 4.1:-34658800 None:intergenic
GTTCTCCAAAGAAGAATCCG+AGG 0.647399 4.1:+34659135 MS.gene056347:CDS
AACAGTTGTCCTAATCCTCG+CGG 0.657090 4.1:-34659553 None:intergenic
TAGCACTCTAGACCAAGCAA+TGG 0.660595 4.1:-34657768 None:intergenic
GTGTCACTCACACAATCCGA+GGG 0.662250 4.1:-34658113 None:intergenic
TTAAATCTGAATTGCACAGG+AGG 0.663041 4.1:+34658378 MS.gene056347:CDS
GTACCGATGATATGCGTCGT+CGG 0.669538 4.1:-34657620 None:intergenic
TTTCGGTAATGTAAATTACG+GGG 0.671497 4.1:-34659603 None:intergenic
AAGAATCCGAGGATCTTAAG+GGG 0.674534 4.1:+34659146 MS.gene056347:CDS
ACTTTCACTCATGAACACCC+AGG 0.690909 4.1:+34656991 MS.gene056347:CDS
TGGTTATGCATTTATCCGCG+AGG 0.715115 4.1:+34659538 MS.gene056347:CDS
GATGTTGATGCTGCACAACG+AGG 0.729333 4.1:+34658777 MS.gene056347:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTATTTATGACTATTTA+GGG + chr4.1:34658154-34658173 MS.gene056347:intron 10.0%
!!! ATTACAATTTTTTTTTGAAA+AGG - chr4.1:34657344-34657363 None:intergenic 10.0%
!! AATAATAGGTAAATGTAAAA+AGG - chr4.1:34659931-34659950 None:intergenic 15.0%
!! AATGATAAAATATATACCAT+TGG - chr4.1:34659403-34659422 None:intergenic 15.0%
!! AGCAATATATTATTCAAATT+CGG + chr4.1:34658505-34658524 MS.gene056347:intron 15.0%
!! AGTTTATAAGAAATTTATGA+TGG - chr4.1:34657852-34657871 None:intergenic 15.0%
!! CTATTTATTTATGACTATTT+AGG + chr4.1:34658153-34658172 MS.gene056347:intron 15.0%
!! TCTCATATATAATAATGAAA+TGG + chr4.1:34657557-34657576 MS.gene056347:intron 15.0%
!!! ATTTCAAAATTTTGGTTATT+TGG - chr4.1:34658679-34658698 None:intergenic 15.0%
!!! TAATAGTATCATGTTTTTTT+TGG + chr4.1:34657058-34657077 MS.gene056347:intron 15.0%
!!! TTTAAAATTTTGTAGTATGA+AGG + chr4.1:34656928-34656947 MS.gene056347:intron 15.0%
!! AAAACTTATGCTATATATGT+TGG - chr4.1:34657481-34657500 None:intergenic 20.0%
!! AATCATAAAGGATTTGAATA+TGG + chr4.1:34657730-34657749 MS.gene056347:CDS 20.0%
!! AGAAGTAATTAAAAATAACC+TGG - chr4.1:34657012-34657031 None:intergenic 20.0%
!! GAAGTAATTAAAAATAACCT+GGG - chr4.1:34657011-34657030 None:intergenic 20.0%
!! GACTGCATATAATTAAAAAA+AGG - chr4.1:34659508-34659527 None:intergenic 20.0%
!! TAAATTGTTAGATTAAGAGA+GGG + chr4.1:34657161-34657180 MS.gene056347:intron 20.0%
!!! AATAGAGTTATTACCATTTT+CGG - chr4.1:34659623-34659642 None:intergenic 20.0%
!!! ATGTAAACTTCATTTGATTT+TGG + chr4.1:34658892-34658911 MS.gene056347:intron 20.0%
!!! ATTTTCGGTAATGTAAATTA+CGG - chr4.1:34659608-34659627 None:intergenic 20.0%
!!! CATTTTGCATTTTTTTCTAT+AGG + chr4.1:34659041-34659060 MS.gene056347:intron 20.0%
!!! CATTTTTTTCTATAGGTATA+TGG + chr4.1:34659048-34659067 MS.gene056347:intron 20.0%
!!! TTTTATGATTAACAATCACA+TGG - chr4.1:34657996-34658015 None:intergenic 20.0%
! AAATGTTGCACTAAATTGAA+TGG - chr4.1:34657320-34657339 None:intergenic 25.0%
! AAGTGACAAAATCAATTGAT+AGG + chr4.1:34659703-34659722 MS.gene056347:intron 25.0%
! AATAGCTCAAGATTGAAAAT+TGG - chr4.1:34658138-34658157 None:intergenic 25.0%
! ATAGGTAAATGTAAAAAGGA+AGG - chr4.1:34659927-34659946 None:intergenic 25.0%
! CATAAATCACCAATGTATAA+GGG - chr4.1:34657825-34657844 None:intergenic 25.0%
! CATCTCAATATGGTATAATA+GGG + chr4.1:34658706-34658725 MS.gene056347:CDS 25.0%
! GATTCTGTAAGTATCTAAAA+GGG - chr4.1:34659770-34659789 None:intergenic 25.0%
! GTAAATTGTTAGATTAAGAG+AGG + chr4.1:34657160-34657179 MS.gene056347:intron 25.0%
! GTAATTTACATTACCGAAAA+TGG + chr4.1:34659607-34659626 MS.gene056347:CDS 25.0%
! TAGGTAAATGTAAAAAGGAA+GGG - chr4.1:34659926-34659945 None:intergenic 25.0%
! TATAGTAGTTTATTCCTTCA+CGG - chr4.1:34657908-34657927 None:intergenic 25.0%
! TATGCATAAAGGTCAATAAT+AGG - chr4.1:34659945-34659964 None:intergenic 25.0%
! TATGTATTTCAGGAAGATAT+TGG + chr4.1:34657707-34657726 MS.gene056347:intron 25.0%
! TCTTATAAACTATTTGCAGA+AGG + chr4.1:34657861-34657880 MS.gene056347:intron 25.0%
! TTAAACTTAATGCAACTTGA+TGG + chr4.1:34657372-34657391 MS.gene056347:intron 25.0%
! TTACAAAACTATGACTTACT+TGG - chr4.1:34657794-34657813 None:intergenic 25.0%
! TTCATATTACTTTGCACAAA+TGG - chr4.1:34658985-34659004 None:intergenic 25.0%
!! CTTTTCCTTAATGATGTATT+TGG + chr4.1:34657395-34657414 MS.gene056347:intron 25.0%
!! TTTTCGGTAATGTAAATTAC+GGG - chr4.1:34659607-34659626 None:intergenic 25.0%
!!! ACCAAAATTTTGAAATATGC+AGG + chr4.1:34658683-34658702 MS.gene056347:intron 25.0%
!!! CATGTTTTTTTTGGTAACAT+GGG + chr4.1:34657067-34657086 MS.gene056347:intron 25.0%
!!! TCATGTTTTTTTTGGTAACA+TGG + chr4.1:34657066-34657085 MS.gene056347:intron 25.0%
AAAACAGAAGACCTGATATT+TGG - chr4.1:34658481-34658500 None:intergenic 30.0%
AAAATAAGAGATGGAGGTAA+TGG + chr4.1:34657589-34657608 MS.gene056347:CDS 30.0%
AAATATATACCATTGGACCA+AGG - chr4.1:34659396-34659415 None:intergenic 30.0%
AAGAGGATGAGTAATAGTTT+AGG - chr4.1:34659189-34659208 None:intergenic 30.0%
AATATATACCATTGGACCAA+GGG - chr4.1:34659395-34659414 None:intergenic 30.0%
AATATTCCTCCCTTATACAT+TGG + chr4.1:34657813-34657832 MS.gene056347:intron 30.0%
ACTATACAAGACCAAATATC+AGG + chr4.1:34658467-34658486 MS.gene056347:CDS 30.0%
ACTTGATCTTCTGTAATAGA+AGG + chr4.1:34658566-34658585 MS.gene056347:intron 30.0%
AGTTACGTAGCATATATGAA+TGG + chr4.1:34657531-34657550 MS.gene056347:intron 30.0%
ATAATCATTGAGAACAATGC+AGG + chr4.1:34658015-34658034 MS.gene056347:intron 30.0%
ATATTATTTGGACATGTTGC+AGG - chr4.1:34657099-34657118 None:intergenic 30.0%
ATCAATAACTAGAAGCTTAG+AGG - chr4.1:34659861-34659880 None:intergenic 30.0%
ATTGACCTTTATGCATAACA+GGG + chr4.1:34659948-34659967 MS.gene056347:intron 30.0%
ATTGTTGGATAACCATGAAT+GGG + chr4.1:34660005-34660024 MS.gene056347:CDS 30.0%
CAGATATGATCATGATAATC+TGG + chr4.1:34658859-34658878 MS.gene056347:intron 30.0%
CCATAAATCACCAATGTATA+AGG - chr4.1:34657826-34657845 None:intergenic 30.0%
CGATTCTGTAAGTATCTAAA+AGG - chr4.1:34659771-34659790 None:intergenic 30.0%
CTTGATCTTCTGTAATAGAA+GGG + chr4.1:34658567-34658586 MS.gene056347:intron 30.0%
CTTGCAGAAAAAATAAGAGA+TGG + chr4.1:34657580-34657599 MS.gene056347:intron 30.0%
GATAAGAGAATCAGTTTGTA+TGG - chr4.1:34659251-34659270 None:intergenic 30.0%
GCATCTCAATATGGTATAAT+AGG + chr4.1:34658705-34658724 MS.gene056347:CDS 30.0%
GGAAGATATTGGAATCATAA+AGG + chr4.1:34657718-34657737 MS.gene056347:intron 30.0%
TAGATCACTCTATGTATTTC+AGG + chr4.1:34657697-34657716 MS.gene056347:intron 30.0%
TATATGGATCCAATCACAAA+AGG + chr4.1:34659064-34659083 MS.gene056347:CDS 30.0%
TATTGACCTTTATGCATAAC+AGG + chr4.1:34659947-34659966 MS.gene056347:intron 30.0%
TCATTTATGCCCTAAATGAA+CGG - chr4.1:34657249-34657268 None:intergenic 30.0%
TTCATATATGCTACGTAACT+AGG - chr4.1:34657531-34657550 None:intergenic 30.0%
TTGCACCAAATACATCATTA+AGG - chr4.1:34657403-34657422 None:intergenic 30.0%
TTTCGGTAATGTAAATTACG+GGG - chr4.1:34659606-34659625 None:intergenic 30.0%
! ATATGTGACACTTTTCCATT+GGG + chr4.1:34658047-34658066 MS.gene056347:CDS 30.0%
! ATATTTTGCATGGTCATTGT+TGG + chr4.1:34659990-34660009 MS.gene056347:CDS 30.0%
! ATCTTGCAGAGATGATTTTA+GGG + chr4.1:34658224-34658243 MS.gene056347:intron 30.0%
! ATGTAAAGGGATATTTTGCA+TGG + chr4.1:34659980-34659999 MS.gene056347:CDS 30.0%
! CCTTATACATTGGTGATTTA+TGG + chr4.1:34657823-34657842 MS.gene056347:intron 30.0%
! GCCTGCATATTTCAAAATTT+TGG - chr4.1:34658687-34658706 None:intergenic 30.0%
! TCATTTAGGGCATAAATGAA+CGG + chr4.1:34657250-34657269 MS.gene056347:intron 30.0%
!! GCTTCTAGTTATTGATTTGT+AGG + chr4.1:34659865-34659884 MS.gene056347:intron 30.0%
!! TCTCGTGTTATTTGAATTTC+AGG - chr4.1:34658547-34658566 None:intergenic 30.0%
!!! TTTCTTGGCAAGATTTTTTG+AGG - chr4.1:34660128-34660147 None:intergenic 30.0%
AAATCACCAATGTATAAGGG+AGG - chr4.1:34657822-34657841 None:intergenic 35.0%
AAATCATCTCTGCAAGATCA+CGG - chr4.1:34658222-34658241 None:intergenic 35.0%
AAGAGAATCAGTTTGTATGG+TGG - chr4.1:34659248-34659267 None:intergenic 35.0%
AATATGCAGGCATCTCAATA+TGG + chr4.1:34658696-34658715 MS.gene056347:intron 35.0%
AATTCATGCCCGTTCATTTA+GGG + chr4.1:34657237-34657256 MS.gene056347:intron 35.0%
ACACAAAAGCTACAAATGAG+AGG - chr4.1:34659453-34659472 None:intergenic 35.0%
AGAGAATCAGTTTGTATGGT+GGG - chr4.1:34659247-34659266 None:intergenic 35.0%
AGCATGAATGCTTATGCATA+TGG + chr4.1:34658324-34658343 MS.gene056347:CDS 35.0%
ATATATACCATTGGACCAAG+GGG - chr4.1:34659394-34659413 None:intergenic 35.0%
ATCGACGAAATATTGGCAAA+TGG + chr4.1:34657936-34657955 MS.gene056347:CDS 35.0%
ATGGAAAAGGACCAAGTATT+TGG + chr4.1:34656965-34656984 MS.gene056347:CDS 35.0%
ATTATGTTCAAGCTGCAATC+AGG + chr4.1:34659812-34659831 MS.gene056347:CDS 35.0%
CATTGTTGGATAACCATGAA+TGG + chr4.1:34660004-34660023 MS.gene056347:CDS 35.0%
CATTTATGCCCTAAATGAAC+GGG - chr4.1:34657248-34657267 None:intergenic 35.0%
CTATATATGTTGGATCTCCA+AGG - chr4.1:34657471-34657490 None:intergenic 35.0%
GAACTTTGCTACAAGGAATT+TGG + chr4.1:34658255-34658274 MS.gene056347:CDS 35.0%
GCAGAAAAAATAAGAGATGG+AGG + chr4.1:34657583-34657602 MS.gene056347:intron 35.0%
GCATGAATGCTTATGCATAT+GGG + chr4.1:34658325-34658344 MS.gene056347:CDS 35.0%
GGATTACTTTGAATGAACCA+TGG + chr4.1:34658295-34658314 MS.gene056347:CDS 35.0%
TGGAAAAGGACCAAGTATTT+GGG + chr4.1:34656966-34656985 MS.gene056347:CDS 35.0%
TGGTTAAATCTGAATTGCAC+AGG + chr4.1:34658375-34658394 MS.gene056347:CDS 35.0%
TTAAATCTGAATTGCACAGG+AGG + chr4.1:34658378-34658397 MS.gene056347:CDS 35.0%
! ACTCGTCTTTGTGGATTATA+AGG + chr4.1:34660053-34660072 MS.gene056347:CDS 35.0%
! CATATGTGACACTTTTCCAT+TGG + chr4.1:34658046-34658065 MS.gene056347:CDS 35.0%
! CTACAAGGAATTTGGTGATA+GGG + chr4.1:34658263-34658282 MS.gene056347:CDS 35.0%
! CTTACAGATTTTCCATTGCT+TGG + chr4.1:34657756-34657775 MS.gene056347:CDS 35.0%
! GATCTTGCAGAGATGATTTT+AGG + chr4.1:34658223-34658242 MS.gene056347:intron 35.0%
! GATTCTTCTTTGGAGAACTT+TGG - chr4.1:34659133-34659152 None:intergenic 35.0%
! GGATAGTTTCCTTTTGTGAT+TGG - chr4.1:34659076-34659095 None:intergenic 35.0%
! TTGGTGATAGGGTAAAACAT+TGG + chr4.1:34658274-34658293 MS.gene056347:CDS 35.0%
!! CAATGGAAAAGTGTCACATA+TGG - chr4.1:34658048-34658067 None:intergenic 35.0%
!! GTGAAAGTATCCCAAATACT+TGG - chr4.1:34656979-34656998 None:intergenic 35.0%
AACAGCTTCTTCTGCATTTC+AGG + chr4.1:34656793-34656812 MS.gene056347:intron 40.0%
AACATCTTCTTTCGCTTGAG+AGG - chr4.1:34658763-34658782 None:intergenic 40.0%
AAGAATCCGAGGATCTTAAG+GGG + chr4.1:34659146-34659165 MS.gene056347:CDS 40.0%
AAGATCCTCGGATTCTTCTT+TGG - chr4.1:34659143-34659162 None:intergenic 40.0%
ACCTTCCCTGTTATGCATAA+AGG - chr4.1:34659956-34659975 None:intergenic 40.0%
ACCTTTATGCATAACAGGGA+AGG + chr4.1:34659952-34659971 MS.gene056347:intron 40.0%
AGAAGAATCCGAGGATCTTA+AGG + chr4.1:34659144-34659163 MS.gene056347:CDS 40.0%
AGCTCCTTTCTTTATGCTTG+TGG + chr4.1:34657281-34657300 MS.gene056347:intron 40.0%
ATATGCAGTCTGCTTCAAGT+TGG + chr4.1:34659518-34659537 MS.gene056347:intron 40.0%
CAACCTCATCGACGAAATAT+TGG + chr4.1:34657929-34657948 MS.gene056347:CDS 40.0%
CGAAGATTGAGTAGTGAAGA+AGG - chr4.1:34656722-34656741 None:intergenic 40.0%
CTATGCTGAACTTTGCTACA+AGG + chr4.1:34658248-34658267 MS.gene056347:CDS 40.0%
CTCATACCAATGAGAGAGTA+AGG - chr4.1:34658736-34658755 None:intergenic 40.0%
GAAGAATCCGAGGATCTTAA+GGG + chr4.1:34659145-34659164 MS.gene056347:CDS 40.0%
GAATTCATGCCCGTTCATTT+AGG + chr4.1:34657236-34657255 MS.gene056347:intron 40.0%
GATAACCATGAATGGGAATC+TGG + chr4.1:34660012-34660031 MS.gene056347:CDS 40.0%
GATCCCACAAGCATAAAGAA+AGG - chr4.1:34657288-34657307 None:intergenic 40.0%
GCAGCATTTGAAGATGGAAA+AGG + chr4.1:34656952-34656971 MS.gene056347:CDS 40.0%
GCTCCTTTCTTTATGCTTGT+GGG + chr4.1:34657282-34657301 MS.gene056347:intron 40.0%
GGATTACCTTACTCTCTCAT+TGG + chr4.1:34658727-34658746 MS.gene056347:CDS 40.0%
GTTTAGGCCTAGAAAATCGA+AGG - chr4.1:34659173-34659192 None:intergenic 40.0%
TAAGTAGCTTCTGAATGCCT+TGG + chr4.1:34657451-34657470 MS.gene056347:intron 40.0%
TCTGTAATAGAAGGGCAAGT+CGG + chr4.1:34658575-34658594 MS.gene056347:intron 40.0%
TTCGGGAACAGAACCATATT+TGG + chr4.1:34658404-34658423 MS.gene056347:CDS 40.0%
TTGCCAATATTTCGTCGATG+AGG - chr4.1:34657935-34657954 None:intergenic 40.0%
TTTGCAGAAGGAAAGCTTAG+TGG + chr4.1:34657873-34657892 MS.gene056347:intron 40.0%
! AAAAGCATGCGATCTTTGGT+TGG + chr4.1:34659097-34659116 MS.gene056347:CDS 40.0%
! ACCAAAGATCGCATGCTTTT+TGG - chr4.1:34659097-34659116 None:intergenic 40.0%
! AGGGAAGGTGTGAATGTAAA+GGG + chr4.1:34659967-34659986 MS.gene056347:intron 40.0%
! GATTCTTCGAGTGACAGTTT+TGG - chr4.1:34659748-34659767 None:intergenic 40.0%
! GCTACAAGGAATTTGGTGAT+AGG + chr4.1:34658262-34658281 MS.gene056347:CDS 40.0%
! TCCAAAAAGCATGCGATCTT+TGG + chr4.1:34659093-34659112 MS.gene056347:CDS 40.0%
!! CAAGGTATGTAGTTTTGCAG+AGG + chr4.1:34657642-34657661 MS.gene056347:intron 40.0%
!!! ATGACGGTTTTTTAAGCCCT+CGG + chr4.1:34658097-34658116 MS.gene056347:CDS 40.0%
AACAGTTGTCCTAATCCTCG+CGG - chr4.1:34659556-34659575 None:intergenic 45.0%
AACCAATCTGAACATCGACC+CGG - chr4.1:34658360-34658379 None:intergenic 45.0%
AATTGCACAGGAGGTGATTC+GGG + chr4.1:34658387-34658406 MS.gene056347:CDS 45.0%
ACCATCCAAACATGAAGTCG+AGG - chr4.1:34658803-34658822 None:intergenic 45.0%
ACCGTCATACTCATCCTCTA+AGG - chr4.1:34658085-34658104 None:intergenic 45.0%
ACTCTCTCATTGGTATGAGC+CGG + chr4.1:34658737-34658756 MS.gene056347:CDS 45.0%
ACTTTCACTCATGAACACCC+AGG + chr4.1:34656991-34657010 MS.gene056347:CDS 45.0%
GAAACAGGTCCGATTGAGAT+CGG - chr4.1:34656753-34656772 None:intergenic 45.0%
GAAACGACACCCAAAACTCT+CGG + chr4.1:34660083-34660102 MS.gene056347:CDS 45.0%
GAATTGCACAGGAGGTGATT+CGG + chr4.1:34658386-34658405 MS.gene056347:CDS 45.0%
GCCTTAGAGGATGAGTATGA+CGG + chr4.1:34658081-34658100 MS.gene056347:CDS 45.0%
GGCATAAATGAACGGACATG+TGG + chr4.1:34657258-34657277 MS.gene056347:intron 45.0%
GTTCTCCAAAGAAGAATCCG+AGG + chr4.1:34659135-34659154 MS.gene056347:CDS 45.0%
TAGCACTCTAGACCAAGCAA+TGG - chr4.1:34657771-34657790 None:intergenic 45.0%
TCGATGACAGTTCATCGTGA+TGG + chr4.1:34659364-34659383 MS.gene056347:intron 45.0%
TGAAGCCAGATTCCCATTCA+TGG - chr4.1:34660020-34660039 None:intergenic 45.0%
TGCATATGGGAAGTTTGCAC+CGG + chr4.1:34658338-34658357 MS.gene056347:CDS 45.0%
TGCATTTATCCGCGAGGATT+AGG + chr4.1:34659544-34659563 MS.gene056347:CDS 45.0%
TGGTTATGCATTTATCCGCG+AGG + chr4.1:34659538-34659557 MS.gene056347:CDS 45.0%
! AACCAATGTGCCGAGAGTTT+TGG - chr4.1:34660096-34660115 None:intergenic 45.0%
! AAGGGGTCCTTCGATTTTCT+AGG + chr4.1:34659163-34659182 MS.gene056347:CDS 45.0%
! ACCAATGTGCCGAGAGTTTT+GGG - chr4.1:34660095-34660114 None:intergenic 45.0%
! CAGGGAAGGTGTGAATGTAA+AGG + chr4.1:34659966-34659985 MS.gene056347:intron 45.0%
! CTTTGGAGAACTTTGGTAGC+CGG - chr4.1:34659126-34659145 None:intergenic 45.0%
! GAAGGTGCAGCATTTGAAGA+TGG + chr4.1:34656946-34656965 MS.gene056347:CDS 45.0%
! GCGACTTTTCCGATCTCAAT+CGG + chr4.1:34656741-34656760 MS.gene056347:CDS 45.0%
! GCTTTACGATGCAAGTTGGA+CGG - chr4.1:34657206-34657225 None:intergenic 45.0%
! TGGTAGTGTGCAGCCAAATA+TGG - chr4.1:34658420-34658439 None:intergenic 45.0%
!! ATCAGTTTGTATGGTGGGTC+TGG - chr4.1:34659242-34659261 None:intergenic 45.0%
!! GTTGGCTTTACGATGCAAGT+TGG - chr4.1:34657210-34657229 None:intergenic 45.0%
!! TACTCATCCTCTAAGGCTTG+AGG - chr4.1:34658078-34658097 None:intergenic 45.0%
!! AATAACATATTAATATTATT+TGG - chr4.1:34657111-34657130 None:intergenic 5.0%
!!! ATATTTTAGAAATAATATTT+TGG - chr4.1:34659477-34659496 None:intergenic 5.0%
ACCCAAAACTCTCGGCACAT+TGG + chr4.1:34660091-34660110 MS.gene056347:CDS 50.0%
AGGTGCATCAGCAGCATAAG+AGG - chr4.1:34659206-34659225 None:intergenic 50.0%
CAGCAGCATGAGCAAGAAGT+TGG - chr4.1:34658440-34658459 None:intergenic 50.0%
CGACGCATATCATCGGTACA+AGG + chr4.1:34657624-34657643 MS.gene056347:CDS 50.0%
GAAGGACCCCTTAAGATCCT+CGG - chr4.1:34659155-34659174 None:intergenic 50.0%
GATGTTGATGCTGCACAACG+AGG + chr4.1:34658777-34658796 MS.gene056347:CDS 50.0%
GCAGAAGGAAAGCTTAGTGG+AGG + chr4.1:34657876-34657895 MS.gene056347:intron 50.0%
GCATATGGGAAGTTTGCACC+GGG + chr4.1:34658339-34658358 MS.gene056347:CDS 50.0%
GCATTCATGCTCACACTCCA+TGG - chr4.1:34658315-34658334 None:intergenic 50.0%
GCCTCGACTTCATGTTTGGA+TGG + chr4.1:34658799-34658818 MS.gene056347:CDS 50.0%
GCTGTTCCGAAAACAAAGCC+TGG - chr4.1:34656779-34656798 None:intergenic 50.0%
GGGCATGAATTCGCTACAGT+TGG - chr4.1:34657228-34657247 None:intergenic 50.0%
GTTCCGAAAACAAAGCCTGG+CGG - chr4.1:34656776-34656795 None:intergenic 50.0%
TAGCCGACGACGCATATCAT+CGG + chr4.1:34657617-34657636 MS.gene056347:CDS 50.0%
TCTTCTTTCGCTTGAGAGGC+CGG - chr4.1:34658759-34658778 None:intergenic 50.0%
TGCGATCTTTGGTTGGAAGC+CGG + chr4.1:34659104-34659123 MS.gene056347:CDS 50.0%
TTCCGAAAACAAAGCCTGGC+GGG - chr4.1:34656775-34656794 None:intergenic 50.0%
! AGTGGAGGTGTAAACCGTGA+AGG + chr4.1:34657891-34657910 MS.gene056347:CDS 50.0%
! GCGATTTGGACTCGTCTTTG+TGG + chr4.1:34660044-34660063 MS.gene056347:CDS 50.0%
! GTACCGATGATATGCGTCGT+CGG - chr4.1:34657623-34657642 None:intergenic 50.0%
! GTGTCACTCACACAATCCGA+GGG - chr4.1:34658116-34658135 None:intergenic 50.0%
! TCTGGCTTCAGCTTGCGATT+TGG + chr4.1:34660030-34660049 MS.gene056347:CDS 50.0%
!!! TTCCCGCCAGGCTTTGTTTT+CGG + chr4.1:34656770-34656789 MS.gene056347:intron 50.0%
AACAAAGCCTGGCGGGAAAC+AGG - chr4.1:34656768-34656787 None:intergenic 55.0%
AGGCTTGAGGAACGTCCCAA+TGG - chr4.1:34658065-34658084 None:intergenic 55.0%
CACCGGGTCGATGTTCAGAT+TGG + chr4.1:34658355-34658374 MS.gene056347:CDS 55.0%
CGAGGCCTCGACTTCATGTT+TGG + chr4.1:34658795-34658814 MS.gene056347:CDS 55.0%
GGACGTTCCTCAAGCCTTAG+AGG + chr4.1:34658068-34658087 MS.gene056347:CDS 55.0%
!! GGTGTCACTCACACAATCCG+AGG - chr4.1:34658117-34658136 None:intergenic 55.0%
AATCGGACCTGTTTCCCGCC+AGG + chr4.1:34656758-34656777 MS.gene056347:intron 60.0%
CATCGTGATGGCAAGCCCCT+TGG + chr4.1:34659376-34659395 MS.gene056347:CDS 60.0%
GGTCTGGCATCGCGTAAGTG+AGG - chr4.1:34659226-34659245 None:intergenic 60.0%
TGGCAAGCCCCTTGGTCCAA+TGG + chr4.1:34659384-34659403 MS.gene056347:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 34656708 34660161 34656708 ID=MS.gene056347
chr4.1 mRNA 34656708 34660161 34656708 ID=MS.gene056347.t1;Parent=MS.gene056347
chr4.1 exon 34656708 34656767 34656708 ID=MS.gene056347.t1.exon1;Parent=MS.gene056347.t1
chr4.1 CDS 34656708 34656767 34656708 ID=cds.MS.gene056347.t1;Parent=MS.gene056347.t1
chr4.1 exon 34656943 34657012 34656943 ID=MS.gene056347.t1.exon2;Parent=MS.gene056347.t1
chr4.1 CDS 34656943 34657012 34656943 ID=cds.MS.gene056347.t1;Parent=MS.gene056347.t1
chr4.1 exon 34657587 34657645 34657587 ID=MS.gene056347.t1.exon3;Parent=MS.gene056347.t1
chr4.1 CDS 34657587 34657645 34657587 ID=cds.MS.gene056347.t1;Parent=MS.gene056347.t1
chr4.1 exon 34657719 34657794 34657719 ID=MS.gene056347.t1.exon4;Parent=MS.gene056347.t1
chr4.1 CDS 34657719 34657794 34657719 ID=cds.MS.gene056347.t1;Parent=MS.gene056347.t1
chr4.1 exon 34657880 34657957 34657880 ID=MS.gene056347.t1.exon5;Parent=MS.gene056347.t1
chr4.1 CDS 34657880 34657957 34657880 ID=cds.MS.gene056347.t1;Parent=MS.gene056347.t1
chr4.1 exon 34658037 34658124 34658037 ID=MS.gene056347.t1.exon6;Parent=MS.gene056347.t1
chr4.1 CDS 34658037 34658124 34658037 ID=cds.MS.gene056347.t1;Parent=MS.gene056347.t1
chr4.1 exon 34658233 34658488 34658233 ID=MS.gene056347.t1.exon7;Parent=MS.gene056347.t1
chr4.1 CDS 34658233 34658488 34658233 ID=cds.MS.gene056347.t1;Parent=MS.gene056347.t1
chr4.1 exon 34658705 34658820 34658705 ID=MS.gene056347.t1.exon8;Parent=MS.gene056347.t1
chr4.1 CDS 34658705 34658820 34658705 ID=cds.MS.gene056347.t1;Parent=MS.gene056347.t1
chr4.1 exon 34659063 34659280 34659063 ID=MS.gene056347.t1.exon9;Parent=MS.gene056347.t1
chr4.1 CDS 34659063 34659280 34659063 ID=cds.MS.gene056347.t1;Parent=MS.gene056347.t1
chr4.1 exon 34659374 34659405 34659374 ID=MS.gene056347.t1.exon10;Parent=MS.gene056347.t1
chr4.1 CDS 34659374 34659405 34659374 ID=cds.MS.gene056347.t1;Parent=MS.gene056347.t1
chr4.1 exon 34659526 34659628 34659526 ID=MS.gene056347.t1.exon11;Parent=MS.gene056347.t1
chr4.1 CDS 34659526 34659628 34659526 ID=cds.MS.gene056347.t1;Parent=MS.gene056347.t1
chr4.1 exon 34659725 34659833 34659725 ID=MS.gene056347.t1.exon12;Parent=MS.gene056347.t1
chr4.1 CDS 34659725 34659833 34659725 ID=cds.MS.gene056347.t1;Parent=MS.gene056347.t1
chr4.1 exon 34659969 34660161 34659969 ID=MS.gene056347.t1.exon13;Parent=MS.gene056347.t1
chr4.1 CDS 34659969 34660161 34659969 ID=cds.MS.gene056347.t1;Parent=MS.gene056347.t1
Gene Sequence

>MS.gene056347

ATGCTTTTGAACCTTCTTCACTACTCAATCTTCGCGACTTTTCCGATCTCAATCGGACCTGTTTCCCGCCAGGCTTTGTTTTCGGAACAGCTTCTTCTGCATTTCAGGCATGCACAAACAAACATCTTTCAATTTTATTATTATTATTTATAACATGCATGCAATGTAGAAATGTAGTTATTAGAGATTTGATTTTGTGTAATGATTAAGTTTCATGTTATTTAAAATTTTGTAGTATGAAGGTGCAGCATTTGAAGATGGAAAAGGACCAAGTATTTGGGATACTTTCACTCATGAACACCCAGGTTATTTTTAATTACTTCTCTTTCATTTTGCAATTTTAAAAAAATTAATAGTATCATGTTTTTTTTGGTAACATGGGTTCGAACCTGCAACATGTCCAAATAATATTAATATGTTATTTTACATCACGCTTTATTGCACATCTGATCGTAAATTGTTAGATTAAGAGAGGGAAACTCTATCTCAATATAACCGTCCAACTTGCATCGTAAAGCCAACTGTAGCGAATTCATGCCCGTTCATTTAGGGCATAAATGAACGGACATGTGGAGCTCCTTTCTTTATGCTTGTGGGATCTTGCATGTTCCATTCAATTTAGTGCAACATTTGCCTTTTCAAAAAAAAATTGTAATAATAATAATTAAACTTAATGCAACTTGATGGCTTTTCCTTAATGATGTATTTGGTGCAAGAATCATGATCAACAGTCATTTTCAGCATAAGTAGCTTCTGAATGCCTTGGAGATCCAACATATATAGCATAAGTTTTTGCTTGCTTTACTTGTATATTGATTTGCCTAGTTACGTAGCATATATGAATGGATTTCTCATATATAATAATGAAATGGCTTGCAGAAAAAATAAGAGATGGAGGTAATGGAGATGTAGCCGACGACGCATATCATCGGTACAAGGTATGTAGTTTTGCAGAGGTATATAAAATACAAATGTAACATAGATCATGATAGATCACTCTATGTATTTCAGGAAGATATTGGAATCATAAAGGATTTGAATATGGATGCTTACAGATTTTCCATTGCTTGGTCTAGAGTGCTACCAAGTAAGTCATAGTTTTGTAAATATTCCTCCCTTATACATTGGTGATTTATGGTAACCATCATAAATTTCTTATAAACTATTTGCAGAAGGAAAGCTTAGTGGAGGTGTAAACCGTGAAGGAATAAACTACTATAACAACCTCATCGACGAAATATTGGCAAATGGTAAAATTCATCAAAATTTATTTGTGTTTTCATATCCATGTGATTGTTAATCATAAAATAATCATTGAGAACAATGCAGGTTTGCAACCATATGTGACACTTTTCCATTGGGACGTTCCTCAAGCCTTAGAGGATGAGTATGACGGTTTTTTAAGCCCTCGGATTGTGTGAGTGACACCAATTTTCAATCTTGAGCTATTTATTTATGACTATTTAGGGAAATATTGCAGTAAATAAAATTAATCGATTAACATATGTAATCCGTGATCTTGCAGAGATGATTTTAGGGACTATGCTGAACTTTGCTACAAGGAATTTGGTGATAGGGTAAAACATTGGATTACTTTGAATGAACCATGGAGTGTGAGCATGAATGCTTATGCATATGGGAAGTTTGCACCGGGTCGATGTTCAGATTGGTTAAATCTGAATTGCACAGGAGGTGATTCGGGAACAGAACCATATTTGGCTGCACACTACCAACTTCTTGCTCATGCTGCTGCTACAAAACTATACAAGACCAAATATCAGGTCTTCTGTTTTTAAAAGCAATATATTATTCAAATTCGGCACTGAATCTTTATGTCCTGAAATTCAAATAACACGAGACTTGATCTTCTGTAATAGAAGGGCAAGTCGGACAAAAGTATACACAATTGCTTTCTATTGATAGATCATACTTATTTTTTTAGTGTGAACTTATTAGAGCTGTTTCTAACCAAATAACCAAAATTTTGAAATATGCAGGCATCTCAATATGGTATAATAGGGATTACCTTACTCTCTCATTGGTATGAGCCGGCCTCTCAAGCGAAAGAAGATGTTGATGCTGCACAACGAGGCCTCGACTTCATGTTTGGATGGTAAAGATTAAACTTAACTATTCGTAGCTCAACACTAACAGATATGATCATGATAATCTGGTTAAAATTGAATGTAAACTTCATTTGATTTTGGTTCAATCTGTGTTTTGAACTCAAGAAATAAGTTATGTAGATTTGCTGAATTCAAATGCAACTATATGCCATTTGTGCAAAGTAATATGAAATCAAAGAAGACTAATACATTTTTTTATTGCATCTGCATTTTGCATTTTTTTCTATAGGTATATGGATCCAATCACAAAAGGAAACTATCCAAAAAGCATGCGATCTTTGGTTGGAAGCCGGCTACCAAAGTTCTCCAAAGAAGAATCCGAGGATCTTAAGGGGTCCTTCGATTTTCTAGGCCTAAACTATTACTCATCCTCTTATGCTGCTGATGCACCTCACTTACGCGATGCCAGACCCACCATACAAACTGATTCTCTTATCAATGCTACATGTAATTATCAAATTAAATATAAGCTTGCACTAGTTAATTATACATACTCTAAACTTTATATCTAATCATGCTTTATTATTTTTTCGATGACAGTTCATCGTGATGGCAAGCCCCTTGGTCCAATGGTATATATTTTATCATTCATTAGCATCTAATTTGCATATCAATGCCTCTCATTTGTAGCTTTTGTGTTCCAAAATATTATTTCTAAAATATAACGTGAACCTTTTTTTAATTATATGCAGTCTGCTTCAAGTTGGTTATGCATTTATCCGCGAGGATTAGGACAACTGTTGCTTTACGTCAAGAATAAGTATAACAACCCCGTAATTTACATTACCGAAAATGGTAATAACTCTATTATACTTCTTATTCTCTTTTATATTATATGCATAATACTGAAACTTTAATAAAGCTAATGAAAGTGACAAAATCAATTGATAGGTCGCGATGAGTTCAACGATCCAAAACTGTCACTCGAAGAATCCCTTTTAGATACTTACAGAATCGATTACTTTTATCGTCATCTTTATTATGTTCAAGCTGCAATCAGGTAACTAATGCTTTATCAGAAGAACCTCTAAGCTTCTAGTTATTGATTTGTAGGTTTTATACATATCAATTGACAATGCACTCTTTCTACCCTTCCTTTTTACATTTACCTATTATTGACCTTTATGCATAACAGGGAAGGTGTGAATGTAAAGGGATATTTTGCATGGTCATTGTTGGATAACCATGAATGGGAATCTGGCTTCAGCTTGCGATTTGGACTCGTCTTTGTGGATTATAAGGACGACTTGAAACGACACCCAAAACTCTCGGCACATTGGTTCAAGAATTTCCTCAAAAAATCTTGCCAAGAAAAGTGTTCTGTTTAA
Protein sequence

>MS.gene056347.t1

MLLNLLHYSIFATFPISIGPYEGAAFEDGKGPSIWDTFTHEHPEKIRDGGNGDVADDAYHRYKEDIGIIKDLNMDAYRFSIAWSRVLPKGKLSGGVNREGINYYNNLIDEILANGLQPYVTLFHWDVPQALEDEYDGFLSPRIVDDFRDYAELCYKEFGDRVKHWITLNEPWSVSMNAYAYGKFAPGRCSDWLNLNCTGGDSGTEPYLAAHYQLLAHAAATKLYKTKYQASQYGIIGITLLSHWYEPASQAKEDVDAAQRGLDFMFGWYMDPITKGNYPKSMRSLVGSRLPKFSKEESEDLKGSFDFLGLNYYSSSYAADAPHLRDARPTIQTDSLINATFHRDGKPLGPMSASSWLCIYPRGLGQLLLYVKNKYNNPVIYITENGRDEFNDPKLSLEESLLDTYRIDYFYRHLYYVQAAIREGVNVKGYFAWSLLDNHEWESGFSLRFGLVFVDYKDDLKRHPKLSAHWFKNFLKKSCQEKCSV