AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54593

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54593.t1 MTR_4g066210 98.065 465 9 0 21 485 63 527 0.0 954
MS.gene54593.t1 MTR_4g066240 88.646 458 52 0 21 478 56 513 0.0 849
MS.gene54593.t1 MTR_4g066240 88.453 459 52 1 21 478 56 514 0.0 845
MS.gene54593.t1 MTR_4g066340 79.310 435 90 0 44 478 6 440 0.0 732
MS.gene54593.t1 MTR_4g066580 69.348 460 141 0 21 480 59 518 0.0 699
MS.gene54593.t1 MTR_3g028720 67.826 460 148 0 21 480 58 517 0.0 689
MS.gene54593.t1 MTR_2g098790 69.365 457 140 0 21 477 58 514 0.0 687
MS.gene54593.t1 MTR_4g066630 69.011 455 141 0 21 475 59 513 0.0 686
MS.gene54593.t1 MTR_4g066590 67.462 461 150 0 21 481 53 513 0.0 683
MS.gene54593.t1 MTR_4g066240 88.283 367 43 0 21 387 56 422 0.0 682
MS.gene54593.t1 MTR_6g078450 67.102 459 151 0 21 479 56 514 0.0 681
MS.gene54593.t1 MTR_2g098520 67.615 457 148 0 21 477 54 510 0.0 681
MS.gene54593.t1 MTR_2g098540 67.396 457 149 0 21 477 51 507 0.0 677
MS.gene54593.t1 MTR_2g098540 66.883 462 153 0 16 477 2 463 0.0 677
MS.gene54593.t1 MTR_6g078330 67.396 457 149 0 21 477 57 513 0.0 667
MS.gene54593.t1 MTR_6g078380 69.365 457 139 1 21 476 56 512 0.0 662
MS.gene54593.t1 MTR_4g066640 64.924 459 157 2 21 478 59 514 0.0 649
MS.gene54593.t1 MTR_5g071520 64.592 466 156 1 21 477 59 524 0.0 622
MS.gene54593.t1 MTR_4g066330 63.181 459 141 2 21 477 61 493 0.0 610
MS.gene54593.t1 MTR_3g028720 68.408 402 127 0 21 422 58 459 0.0 608
MS.gene54593.t1 MTR_4g066630 69.077 401 124 0 21 421 59 459 0.0 607
MS.gene54593.t1 MTR_4g066280 62.092 459 146 2 21 477 61 493 0.0 603
MS.gene54593.t1 MTR_6g078330 67.482 409 133 0 69 477 1 409 0.0 600
MS.gene54593.t1 MTR_6g078450 68.159 402 128 0 21 422 56 457 0.0 599
MS.gene54593.t1 MTR_3g026280 59.519 457 180 2 21 477 42 493 0.0 582
MS.gene54593.t1 MTR_4g066580 69.459 370 113 0 21 390 59 428 0.0 566
MS.gene54593.t1 MTR_6g078330 65.995 397 133 1 81 477 15 409 0.0 564
MS.gene54593.t1 MTR_4g066630 69.210 367 113 0 21 387 59 425 0.0 560
MS.gene54593.t1 MTR_3g028720 68.750 368 115 0 21 388 58 425 0.0 559
MS.gene54593.t1 MTR_4g023000 57.987 457 186 3 21 477 44 494 0.0 553
MS.gene54593.t1 MTR_4g067040 68.362 354 112 0 21 374 54 407 0.0 531
MS.gene54593.t1 MTR_2g043200 57.235 463 164 5 21 477 85 519 0.0 527
MS.gene54593.t1 MTR_4g015420 53.945 469 176 6 21 477 85 525 0.0 521
MS.gene54593.t1 MTR_3g026400 53.392 457 207 4 22 477 27 478 0.0 515
MS.gene54593.t1 MTR_3g026400 53.392 457 207 4 22 477 58 509 1.71e-180 516
MS.gene54593.t1 MTR_4g067040 70.213 329 98 0 21 349 54 382 2.50e-180 510
MS.gene54593.t1 MTR_3g028720 69.301 329 101 0 21 349 58 386 1.22e-179 509
MS.gene54593.t1 MTR_4g015460 54.176 467 175 9 21 476 62 500 3.92e-175 502
MS.gene54593.t1 MTR_4g066630 70.440 318 94 0 21 338 59 376 1.05e-174 496
MS.gene54593.t1 MTR_4g083000 52.610 479 213 6 4 476 32 502 1.70e-174 500
MS.gene54593.t1 MTR_3g026620 53.509 456 208 3 22 477 59 510 2.46e-173 497
MS.gene54593.t1 MTR_5g044000 52.838 458 210 4 21 475 66 520 1.73e-170 490
MS.gene54593.t1 MTR_6g078330 67.812 320 103 0 21 340 57 376 4.97e-165 471
MS.gene54593.t1 MTR_4g066640 63.746 331 116 2 21 350 59 386 9.46e-161 461
MS.gene54593.t1 MTR_4g081530 48.643 479 237 6 10 484 34 507 6.78e-155 451
MS.gene54593.t1 MTR_0110s0040 49.234 457 223 6 22 477 64 512 1.00e-153 447
MS.gene54593.t1 MTR_0110s0030 49.015 457 224 6 22 477 64 512 1.33e-153 447
MS.gene54593.t1 MTR_4g131810 48.908 458 229 5 21 475 48 503 1.60e-153 447
MS.gene54593.t1 MTR_4g083000 52.415 414 185 4 69 476 1 408 5.27e-152 439
MS.gene54593.t1 MTR_2g022760 48.596 463 233 5 21 481 56 515 8.13e-150 438
MS.gene54593.t1 MTR_3g033790 46.414 474 234 7 13 482 29 486 1.20e-146 429
MS.gene54593.t1 MTR_3g033810 46.737 475 232 7 13 482 31 489 5.27e-146 427
MS.gene54593.t1 MTR_4g131800 48.164 463 234 6 21 481 536 994 1.51e-143 437
MS.gene54593.t1 MTR_2g079550 45.259 464 243 6 22 481 51 507 1.95e-139 411
MS.gene54593.t1 MTR_7g033860 46.522 460 231 5 22 479 69 515 6.00e-139 410
MS.gene54593.t1 MTR_2g079580 44.828 464 245 6 22 481 53 509 6.69e-139 410
MS.gene54593.t1 MTR_7g068910 44.632 475 233 8 13 477 41 495 1.02e-138 409
MS.gene54593.t1 MTR_2g022710 44.009 459 251 5 21 477 55 509 1.35e-138 409
MS.gene54593.t1 MTR_7g068910 43.711 485 233 9 13 477 41 505 1.29e-135 402
MS.gene54593.t1 MTR_3g026400 61.616 297 111 2 22 318 58 351 7.33e-134 392
MS.gene54593.t1 MTR_4g016920 43.871 465 243 7 22 483 48 497 7.86e-132 392
MS.gene54593.t1 MTR_5g068740 44.252 461 236 8 22 478 48 491 1.72e-131 391
MS.gene54593.t1 MTR_0108s0030 43.696 460 230 4 22 481 24 454 5.50e-130 385
MS.gene54593.t1 MTR_2g022760 46.988 415 215 5 69 481 1 412 8.41e-127 375
MS.gene54593.t1 MTR_1g088610 44.009 459 235 7 22 476 48 488 7.67e-126 380
MS.gene54593.t1 MTR_1g088610 41.176 119 63 2 361 478 503 615 4.55e-18 87.8
MS.gene54593.t1 MTR_7g068900 45.540 426 196 9 64 476 17 419 1.10e-120 362
MS.gene54593.t1 MTR_1g088630 42.495 473 226 10 10 478 4 434 1.31e-120 361
MS.gene54593.t1 MTR_2g022710 43.103 406 226 4 74 477 6 408 9.05e-120 357
MS.gene54593.t1 MTR_7g051510 44.397 464 232 8 22 481 96 537 3.00e-118 357
MS.gene54593.t1 MTR_7g051610 44.469 461 234 6 17 475 91 531 1.85e-116 353
MS.gene54593.t1 MTR_2g022710 42.574 404 227 4 21 422 55 455 1.88e-115 348
MS.gene54593.t1 MTR_2g022710 42.574 404 227 4 21 422 55 455 5.24e-115 347
MS.gene54593.t1 MTR_2g079580 46.612 369 187 5 22 386 53 415 7.89e-115 345
MS.gene54593.t1 MTR_4g016920 42.823 418 222 6 69 483 1 404 1.83e-114 344
MS.gene54593.t1 MTR_7g051650 42.544 456 241 5 22 475 96 532 3.87e-114 347
MS.gene54593.t1 MTR_2g022710 44.173 369 201 4 21 387 55 420 5.03e-113 340
MS.gene54593.t1 MTR_7g051650 42.105 456 239 5 22 475 96 528 1.08e-112 343
MS.gene54593.t1 MTR_0110s0040 51.757 313 147 3 22 334 64 372 9.12e-111 334
MS.gene54593.t1 MTR_2g022710 43.539 356 196 4 21 374 55 407 3.64e-106 322
MS.gene54593.t1 MTR_7g056747 43.750 416 204 5 63 475 9 397 1.28e-105 320
MS.gene54593.t1 MTR_2g022710 48.505 301 152 3 21 321 55 352 1.94e-99 305
MS.gene54593.t1 MTR_1g088630 48.000 300 126 5 22 320 48 318 2.07e-93 288
MS.gene54593.t1 MTR_4g016920 51.984 252 119 1 69 320 1 250 1.86e-92 283
MS.gene54593.t1 MTR_1g088630 51.417 247 114 3 22 267 48 289 3.58e-83 259
MS.gene54593.t1 MTR_0110s0020 50.216 231 109 4 13 241 53 279 1.55e-74 238
MS.gene54593.t1 MTR_5g069350 41.341 179 52 5 315 477 48 189 1.02e-29 115
MS.gene54593.t1 MTR_4g122980 27.751 418 227 13 65 475 183 532 1.65e-29 122
MS.gene54593.t1 MTR_8g038650 45.000 100 55 0 227 326 57 156 5.84e-24 98.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54593.t1 AT5G44640 54.923 457 200 3 22 477 52 503 0.0 540
MS.gene54593.t1 AT2G44450 54.486 457 201 3 22 477 52 502 0.0 537
MS.gene54593.t1 AT5G42260 55.654 451 194 3 22 471 52 497 0.0 536
MS.gene54593.t1 AT2G44480 53.611 457 211 1 22 478 87 542 0.0 534
MS.gene54593.t1 AT2G44480 53.611 457 211 1 22 478 56 511 0.0 533
MS.gene54593.t1 AT3G60130 54.605 456 201 3 22 476 51 501 0.0 517
MS.gene54593.t1 AT2G25630 53.846 455 187 4 22 475 51 483 1.29e-177 507
MS.gene54593.t1 AT5G24550 51.948 462 217 5 21 478 52 512 1.80e-173 498
MS.gene54593.t1 AT5G24540 51.948 462 217 5 21 478 52 512 5.69e-165 477
MS.gene54593.t1 AT3G60140 48.837 473 237 5 10 479 36 506 7.05e-165 478
MS.gene54593.t1 AT1G26560 49.567 462 225 6 21 478 52 509 3.45e-164 474
MS.gene54593.t1 AT2G44480 52.195 410 195 1 69 478 1 409 7.01e-163 467
MS.gene54593.t1 AT1G51470 50.655 458 212 6 22 477 66 511 3.64e-162 469
MS.gene54593.t1 AT5G54570 50.763 459 219 4 21 475 50 505 9.32e-161 466
MS.gene54593.t1 AT1G47600 50.216 462 208 7 22 477 66 511 1.02e-160 465
MS.gene54593.t1 AT2G44460 51.183 465 220 6 21 481 49 510 2.36e-160 467
MS.gene54593.t1 AT1G47600 50.000 462 208 7 22 477 66 510 1.04e-159 462
MS.gene54593.t1 AT2G44470 47.579 475 236 6 10 477 39 507 2.30e-159 464
MS.gene54593.t1 AT3G60130 53.253 415 188 3 63 476 40 449 1.11e-158 458
MS.gene54593.t1 AT3G60120 49.892 463 221 7 21 477 34 491 9.39e-158 459
MS.gene54593.t1 AT2G44490 47.660 470 240 5 21 485 34 502 6.78e-154 449
MS.gene54593.t1 AT3G18080 48.584 459 226 7 22 478 60 510 1.64e-153 447
MS.gene54593.t1 AT5G25980 48.596 463 221 10 29 482 75 529 1.29e-151 443
MS.gene54593.t1 AT5G36890 49.049 473 226 5 13 482 26 486 4.85e-151 440
MS.gene54593.t1 AT5G36890 49.049 473 226 5 13 482 26 486 7.26e-151 439
MS.gene54593.t1 AT3G60130 51.807 415 183 4 63 476 40 438 6.15e-150 436
MS.gene54593.t1 AT2G32860 46.025 478 253 5 10 483 104 580 9.49e-150 441
MS.gene54593.t1 AT2G32860 46.025 478 252 6 10 483 104 579 2.06e-148 437
MS.gene54593.t1 AT5G26000 48.696 460 212 9 29 475 63 511 8.28e-148 433
MS.gene54593.t1 AT3G18070 45.752 459 229 9 22 477 51 492 6.87e-146 427
MS.gene54593.t1 AT1G61820 47.619 462 236 5 21 479 50 508 1.95e-145 426
MS.gene54593.t1 AT3G03640 45.887 462 244 4 21 477 54 514 2.89e-145 427
MS.gene54593.t1 AT3G18070 45.752 459 235 9 22 477 51 498 1.67e-144 424
MS.gene54593.t1 AT5G54570 50.365 411 197 4 69 475 1 408 2.62e-142 416
MS.gene54593.t1 AT1G02850 46.667 465 221 8 22 480 47 490 1.50e-140 413
MS.gene54593.t1 AT3G09260 45.455 462 247 5 22 480 56 515 1.78e-139 411
MS.gene54593.t1 AT1G75940 45.887 462 240 7 22 475 58 517 5.04e-139 410
MS.gene54593.t1 AT5G54570 50.617 405 194 4 21 422 50 451 3.52e-138 405
MS.gene54593.t1 AT5G24540 50.495 404 196 4 21 421 52 454 7.22e-138 405
MS.gene54593.t1 AT2G44470 47.129 418 209 5 10 421 39 450 2.28e-136 401
MS.gene54593.t1 AT1G02850 44.856 486 218 9 22 478 47 511 6.00e-136 402
MS.gene54593.t1 AT3G21370 44.635 466 240 9 22 476 54 512 6.97e-135 400
MS.gene54593.t1 AT1G66280 45.690 464 242 7 22 480 57 515 1.26e-134 399
MS.gene54593.t1 AT2G44450 52.381 357 163 3 122 477 1 351 1.93e-134 392
MS.gene54593.t1 AT5G28510 43.469 467 254 6 22 480 60 524 2.31e-134 399
MS.gene54593.t1 AT1G02850 44.764 487 218 10 22 478 47 512 3.61e-134 398
MS.gene54593.t1 AT4G21760 43.326 457 248 6 21 475 74 521 4.90e-134 398
MS.gene54593.t1 AT4G27830 42.981 463 243 8 21 479 43 488 1.63e-133 395
MS.gene54593.t1 AT1G52400 45.788 463 237 10 22 476 60 516 2.96e-133 396
MS.gene54593.t1 AT1G52400 45.788 463 237 10 22 476 60 516 2.96e-133 396
MS.gene54593.t1 AT3G60140 48.564 383 193 4 10 390 36 416 3.63e-133 393
MS.gene54593.t1 AT5G25980 49.127 401 189 8 29 422 75 467 1.25e-132 392
MS.gene54593.t1 AT5G25980 49.127 401 189 8 29 422 75 467 1.25e-132 392
MS.gene54593.t1 AT1G61810 43.441 465 257 5 21 482 53 514 1.38e-132 394
MS.gene54593.t1 AT1G66270 44.060 463 251 6 22 480 57 515 5.30e-132 392
MS.gene54593.t1 AT1G02850 45.376 465 200 9 22 480 47 463 7.54e-131 387
MS.gene54593.t1 AT1G66270 43.844 463 250 7 22 480 57 513 5.38e-129 385
MS.gene54593.t1 AT5G26000 47.772 404 191 6 29 422 63 456 1.24e-128 381
MS.gene54593.t1 AT1G60090 42.733 461 238 9 21 476 44 483 3.90e-128 382
MS.gene54593.t1 AT4G22100 43.506 462 235 9 21 476 42 483 7.37e-128 381
MS.gene54593.t1 AT4G21760 42.670 457 241 7 21 475 74 511 2.35e-127 381
MS.gene54593.t1 AT2G44480 57.931 290 120 2 22 311 87 374 1.05e-126 374
MS.gene54593.t1 AT1G61810 43.820 445 244 5 21 462 53 494 6.98e-126 377
MS.gene54593.t1 AT4G27820 42.333 463 244 7 21 479 43 486 2.44e-125 375
MS.gene54593.t1 AT1G60090 42.026 464 244 10 21 476 44 490 2.06e-124 372
MS.gene54593.t1 AT1G45191 41.342 462 249 8 16 476 44 484 4.86e-121 363
MS.gene54593.t1 AT1G45191 40.860 465 253 8 16 476 44 490 4.04e-119 359
MS.gene54593.t1 AT3G62750 41.612 459 236 9 21 475 43 473 6.40e-119 358
MS.gene54593.t1 AT1G45191 40.340 471 252 8 16 476 44 495 1.34e-118 358
MS.gene54593.t1 AT3G62750 41.253 463 240 9 21 479 69 503 1.52e-118 358
MS.gene54593.t1 AT2G44470 48.538 342 168 4 10 345 39 378 1.02e-116 349
MS.gene54593.t1 AT1G45191 39.749 478 257 9 16 476 44 507 5.75e-116 352
MS.gene54593.t1 AT5G48375 41.176 442 202 6 29 468 54 439 2.37e-115 347
MS.gene54593.t1 AT1G61810 42.754 414 231 5 72 482 4 414 6.94e-115 345
MS.gene54593.t1 AT4G27830 42.169 415 221 7 69 479 1 400 3.23e-114 343
MS.gene54593.t1 AT2G44460 49.180 366 180 5 120 481 1 364 4.76e-114 343
MS.gene54593.t1 AT3G62740 38.125 480 256 10 21 484 43 497 2.85e-111 338
MS.gene54593.t1 AT1G02850 45.038 393 173 6 22 386 47 424 3.42e-110 333
MS.gene54593.t1 AT1G52400 45.209 407 210 9 22 421 60 460 1.30e-109 333
MS.gene54593.t1 AT4G27820 41.205 415 226 6 69 479 1 401 1.01e-105 322
MS.gene54593.t1 AT4G27820 41.205 415 226 6 69 479 1 401 1.01e-105 322
MS.gene54593.t1 AT1G61820 45.013 371 199 4 112 479 1 369 1.02e-105 320
MS.gene54593.t1 AT1G61810 42.547 369 207 4 21 386 53 419 2.94e-101 310
MS.gene54593.t1 AT3G62750 41.278 407 208 8 21 424 69 447 4.42e-101 311
MS.gene54593.t1 AT2G44480 48.173 301 155 1 178 478 6 305 4.59e-101 306
MS.gene54593.t1 AT3G62750 40.000 415 222 8 69 479 1 392 3.43e-100 307
MS.gene54593.t1 AT3G62750 40.000 415 222 8 69 479 1 392 3.43e-100 307
MS.gene54593.t1 AT1G60270 49.147 293 142 3 21 313 44 329 6.24e-100 305
MS.gene54593.t1 AT3G18070 45.427 328 170 6 153 477 100 421 2.70e-98 302
MS.gene54593.t1 AT1G51490 46.939 343 161 8 13 349 30 357 3.98e-98 301
MS.gene54593.t1 AT3G18070 47.492 299 151 4 22 320 51 343 5.54e-98 300
MS.gene54593.t1 AT3G62740 38.916 406 218 8 21 421 43 423 2.31e-95 295
MS.gene54593.t1 AT3G18070 45.079 315 164 6 166 477 1 309 9.17e-93 284
MS.gene54593.t1 AT3G62750 44.884 303 149 5 21 322 43 328 1.89e-92 286
MS.gene54593.t1 AT3G62750 44.884 303 149 5 21 322 43 328 1.89e-92 286
MS.gene54593.t1 AT1G45191 44.590 305 161 3 16 320 44 340 3.86e-92 286
MS.gene54593.t1 AT1G45191 44.590 305 161 3 16 320 44 340 4.16e-92 286
MS.gene54593.t1 AT3G62750 44.884 303 149 5 21 322 69 354 9.63e-92 286
MS.gene54593.t1 AT1G60270 49.383 243 120 2 71 313 3 242 3.46e-81 254
MS.gene54593.t1 AT1G61810 48.000 250 128 2 21 270 53 300 1.62e-79 250
MS.gene54593.t1 AT3G18070 43.220 236 130 3 153 386 100 333 2.96e-65 214
MS.gene54593.t1 AT3G18070 42.601 223 124 3 166 386 1 221 9.09e-60 196
MS.gene54593.t1 AT5G16580 37.631 287 164 7 147 432 27 299 7.92e-52 178
MS.gene54593.t1 AT3G06510 25.776 419 238 12 64 476 159 510 1.40e-30 125
MS.gene54593.t1 AT3G06510 25.571 438 249 13 64 476 159 544 3.36e-30 124

Find 112 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGAAAAGGACCAAGTATT+TGG 0.197865 4.3:+37777189 MS.gene54593:CDS
GCATCTCAATATGGTATAAT+AGG 0.209400 4.3:+37778929 MS.gene54593:CDS
GACCTTTGCTACAAGGAATT+TGG 0.235205 4.3:+37778479 MS.gene54593:CDS
AAAACAGAAGACCTGATATT+TGG 0.235314 4.3:-37778702 None:intergenic
GTAATTTACATTACCGAAAA+TGG 0.241625 4.3:+37779916 MS.gene54593:CDS
CGATTCTGTAAGTATCTAAA+AGG 0.249841 4.3:-37780077 None:intergenic
TTCGGGAACAGAACCATATT+TGG 0.289048 4.3:+37778628 MS.gene54593:CDS
AATCATAAAGGATTTGAATA+TGG 0.307459 4.3:+37777954 MS.gene54593:CDS
AAGATCCTCGGATTCTTCTT+TGG 0.311879 4.3:-37779408 None:intergenic
AACCAATGTGCCGAAAGTTT+TGG 0.341374 4.3:-37780402 None:intergenic
TGGTAGTGTGCAGCCAAATA+TGG 0.343520 4.3:-37778641 None:intergenic
CGAGGCCTCGACTTCATGTT+TGG 0.348528 4.3:+37779019 MS.gene54593:CDS
GATTCTTCAAGCGACAGTTT+TGG 0.358175 4.3:-37780054 None:intergenic
AGAAGAATCCGAGGATCTTA+AGG 0.358352 4.3:+37779412 MS.gene54593:CDS
GGACGTTCCTCAAGCCTTAG+AGG 0.360927 4.3:+37778292 MS.gene54593:CDS
TGCATTTATCCGCGAGGATT+AGG 0.361716 4.3:+37779853 MS.gene54593:CDS
TCTGGCTTCAGCTTGCGATT+TGG 0.362792 4.3:+37780339 MS.gene54593:CDS
TATGTATTTCAGGAAGATAT+TGG 0.369128 4.3:+37777931 MS.gene54593:intron
TGAAGCCAGATTCCCATTCA+TGG 0.380755 4.3:-37780326 None:intergenic
AAAATAAGAGATGGAGGTAA+TGG 0.386283 4.3:+37777806 MS.gene54593:CDS
GGAAGATATTGGAATCATAA+AGG 0.386661 4.3:+37777942 MS.gene54593:intron
AATTGCACAGGAGGTGATTC+GGG 0.389673 4.3:+37778611 MS.gene54593:CDS
ATCAGTTTGTATGGCGGGTC+TGG 0.391534 4.3:-37779507 None:intergenic
GATAAGAGAATCAGTTTGTA+TGG 0.395420 4.3:-37779516 None:intergenic
GAAACGACACCCAAAACTTT+CGG 0.397446 4.3:+37780392 MS.gene54593:CDS
TGGAAAAGGACCAAGTATTT+GGG 0.404129 4.3:+37777190 MS.gene54593:CDS
CAACCTCATCAACGAACTAT+TGG 0.405447 4.3:+37778151 MS.gene54593:CDS
GATAACCATGAATGGGAATC+TGG 0.405714 4.3:+37780321 MS.gene54593:CDS
GAATTGCACAGGAGGTGATT+CGG 0.408321 4.3:+37778610 MS.gene54593:CDS
CATCTCAATATGGTATAATA+GGG 0.411825 4.3:+37778930 MS.gene54593:CDS
AGGTGCATCAGCAGCATAAG+AGG 0.414938 4.3:-37779471 None:intergenic
TGTAGTAGTTTATTCCTTCA+CGG 0.418155 4.3:-37778127 None:intergenic
AATATGCAGGCATCTCAATA+TGG 0.419382 4.3:+37778920 MS.gene54593:intron
AAGAGGAAGAGTAATAGTTT+AGG 0.433722 4.3:-37779454 None:intergenic
AGGGAAGGTGTGAATGTAAA+GGG 0.435489 4.3:+37780276 MS.gene54593:intron
GCGATTTGGACTCGTCTTTG+TGG 0.435971 4.3:+37780353 MS.gene54593:CDS
ATTATGTTCAAGCTGCAATC+AGG 0.440754 4.3:+37780121 MS.gene54593:CDS
GATCGGAAAAGTCGCGAAGA+TGG 0.446253 4.3:-37776946 None:intergenic
TTTGCAGAAGGAAAGCTTAG+TGG 0.446993 4.3:+37778095 MS.gene54593:intron
ACTATACAAGACCAAATATC+AGG 0.449280 4.3:+37778691 MS.gene54593:CDS
TCTTCTTTCGCTTGAGAGGC+CGG 0.450631 4.3:-37778980 None:intergenic
ATCAACGAACTATTGGCTAA+TGG 0.455297 4.3:+37778158 MS.gene54593:CDS
AGCATGAATGCTTATGCATA+TGG 0.456434 4.3:+37778548 MS.gene54593:CDS
GGATTACCTTACTCTCTCAT+TGG 0.457583 4.3:+37778951 MS.gene54593:CDS
CTTTGGAGAACTTTGGTAGC+CGG 0.461441 4.3:-37779391 None:intergenic
GAAGAATCCGAGGATCTTAA+GGG 0.463005 4.3:+37779413 MS.gene54593:CDS
CACCGGGTCGATGTTCAGAT+TGG 0.463655 4.3:+37778579 MS.gene54593:CDS
ACCGTCATACTCATCCTCTA+AGG 0.470920 4.3:-37778306 None:intergenic
GATTCTTCTTTGGAGAACTT+TGG 0.475194 4.3:-37779398 None:intergenic
TGCATATGGGAAGTTTGCAC+CGG 0.482317 4.3:+37778562 MS.gene54593:CDS
TGCGATCTTTGGTTGGAAGC+CGG 0.484554 4.3:+37779372 MS.gene54593:CDS
CATCGTGATGGCAAGCCCCT+TGG 0.493086 4.3:+37779685 MS.gene54593:CDS
CAGGGAAGGTGTGAATGTAA+AGG 0.498317 4.3:+37780275 MS.gene54593:intron
CTCATACCAATGAGAGAGTA+AGG 0.499326 4.3:-37778957 None:intergenic
AACATCTTCTTTCGCTTGAG+AGG 0.502079 4.3:-37778984 None:intergenic
TTGGTGATAGAGTGAAACAT+TGG 0.506776 4.3:+37778498 MS.gene54593:CDS
AGAGAATCAGTTTGTATGGC+GGG 0.508377 4.3:-37779512 None:intergenic
CTATGCTGACCTTTGCTACA+AGG 0.508555 4.3:+37778472 MS.gene54593:CDS
CAGCAGCATGAGCAAGAAGT+TGG 0.509755 4.3:-37778661 None:intergenic
CATTGTTGGATAACCATGAA+TGG 0.514197 4.3:+37780313 MS.gene54593:CDS
TATATGGATCCAATCACAAA+AGG 0.519336 4.3:+37779332 MS.gene54593:CDS
CACCAAATTCCTTGTAGCAA+AGG 0.520911 4.3:-37778481 None:intergenic
ATTGTTGGATAACCATGAAT+GGG 0.522990 4.3:+37780314 MS.gene54593:CDS
TGGTTAAATCTGAATTGCAC+AGG 0.528273 4.3:+37778599 MS.gene54593:CDS
ACTCTCTCATTGGTATGAGC+CGG 0.532738 4.3:+37778961 MS.gene54593:CDS
TGGCAAGCCCCTTGGTCCAA+TGG 0.533454 4.3:+37779693 MS.gene54593:CDS
GCAGCATTTGAAGATGGAAA+AGG 0.538475 4.3:+37777176 MS.gene54593:CDS
AAAAGTATGCGATCTTTGGT+TGG 0.540482 4.3:+37779365 MS.gene54593:CDS
GATTCTGTAAGTATCTAAAA+GGG 0.541771 4.3:-37780076 None:intergenic
ACCCAAAACTTTCGGCACAT+TGG 0.543767 4.3:+37780400 MS.gene54593:CDS
AATATATACCATTGGACCAA+GGG 0.544956 4.3:-37779701 None:intergenic
TCGATGACAGTTCATCGTGA+TGG 0.548253 4.3:+37779673 MS.gene54593:intron
GCCTCGACTTCATGTTTGGA+TGG 0.549073 4.3:+37779023 MS.gene54593:CDS
GAAGGTGCAGCATTTGAAGA+TGG 0.552430 4.3:+37777170 MS.gene54593:CDS
GTGAAAGTATCCCAAATACT+TGG 0.559968 4.3:-37777200 None:intergenic
TAGCCAATAGTTCGTTGATG+AGG 0.565702 4.3:-37778154 None:intergenic
ATATGCAGTCTGCTTCAAGT+TGG 0.568943 4.3:+37779827 MS.gene54593:intron
CGAAGATGGAGTAGTGAAGA+AGG 0.570282 4.3:-37776932 None:intergenic
AAAGGACCCCTTAAGATCCT+CGG 0.570685 4.3:-37779420 None:intergenic
CGACGCATATCATCGGTACA+AGG 0.572001 4.3:+37777841 MS.gene54593:CDS
GCATTCATGCTCACACTCCA+TGG 0.576199 4.3:-37778536 None:intergenic
GAAACAGGTCCGATTGAGAT+CGG 0.577440 4.3:-37776963 None:intergenic
CAATGGAAAAGAGTCACATA+TGG 0.580739 4.3:-37778269 None:intergenic
GTTTAGGCCAAGAAAATCAA+AGG 0.589220 4.3:-37779438 None:intergenic
TACTCATCCTCTAAGGCTTG+AGG 0.594134 4.3:-37778299 None:intergenic
TAGCCGACGACGCATATCAT+CGG 0.598194 4.3:+37777834 MS.gene54593:CDS
AAATATATACCATTGGACCA+AGG 0.598574 4.3:-37779702 None:intergenic
CTTGAACATAATAAAGATGA+CGG 0.602302 4.3:-37780110 None:intergenic
GGATTACTTTGAATGAACCA+TGG 0.603853 4.3:+37778519 MS.gene54593:CDS
AGGCTTGAGGAACGTCCCAA+TGG 0.604842 4.3:-37778286 None:intergenic
GCATGAATGCTTATGCATAT+GGG 0.610049 4.3:+37778549 MS.gene54593:CDS
AACCAATCTGAACATCGACC+CGG 0.611784 4.3:-37778581 None:intergenic
GGTGTCACTCACACAATCCG+AGG 0.616673 4.3:-37778338 None:intergenic
GCATATGGGAAGTTTGCACC+GGG 0.626791 4.3:+37778563 MS.gene54593:CDS
GCCTTAGAGGATGAGTATGA+CGG 0.628770 4.3:+37778305 MS.gene54593:CDS
GCAGAAGGAAAGCTTAGTGG+AGG 0.637778 4.3:+37778098 MS.gene54593:intron
ATATATACCATTGGACCAAG+GGG 0.639799 4.3:-37779700 None:intergenic
AGTGGAGGTGTAAACCGTGA+AGG 0.641058 4.3:+37778113 MS.gene54593:CDS
ACCATCCAAACATGAAGTCG+AGG 0.641696 4.3:-37779024 None:intergenic
GTTCTCCAAAGAAGAATCCG+AGG 0.647399 4.3:+37779403 MS.gene54593:CDS
GGTCTGGCATCACGTAAGTG+AGG 0.656924 4.3:-37779491 None:intergenic
AACAGTTGTCCTAATCCTCG+CGG 0.657090 4.3:-37779862 None:intergenic
AAGAGAATCAGTTTGTATGG+CGG 0.658985 4.3:-37779513 None:intergenic
TAGCACTCTAGACCAAGCAA+TGG 0.660595 4.3:-37777992 None:intergenic
GTGTCACTCACACAATCCGA+GGG 0.662250 4.3:-37778337 None:intergenic
TTAAATCTGAATTGCACAGG+AGG 0.663041 4.3:+37778602 MS.gene54593:CDS
GTACCGATGATATGCGTCGT+CGG 0.669538 4.3:-37777837 None:intergenic
TTTCGGTAATGTAAATTACG+GGG 0.671497 4.3:-37779912 None:intergenic
AAGAATCCGAGGATCTTAAG+GGG 0.674534 4.3:+37779414 MS.gene54593:CDS
ACTTTCACTCATGAACACCC+AGG 0.690909 4.3:+37777215 MS.gene54593:CDS
TGGTTATGCATTTATCCGCG+AGG 0.715115 4.3:+37779847 MS.gene54593:CDS
GATGTTGATGCTGCACAACG+AGG 0.729333 4.3:+37779001 MS.gene54593:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACATACTAATATTATT+TGG - chr4.3:37777332-37777351 None:intergenic 10.0%
!!! CAATTTTTTTTTTTTTGAAA+AGG - chr4.3:37777565-37777584 None:intergenic 10.0%
!!! TATTAATATCATGTTTTTTT+TGG + chr4.3:37777279-37777298 MS.gene54593:intron 10.0%
!! AATAATAGGTAAATGTAAAA+AGG - chr4.3:37780240-37780259 None:intergenic 15.0%
!! AATGATAAAATATATACCAT+TGG - chr4.3:37779712-37779731 None:intergenic 15.0%
!! AGCAATATATTATTCAAATT+CGG + chr4.3:37778729-37778748 MS.gene54593:intron 15.0%
!! TCTCATATATATTAATGATA+TGG + chr4.3:37777774-37777793 MS.gene54593:intron 15.0%
!!! ATTTCAAAATTTTGGTTATT+TGG - chr4.3:37778903-37778922 None:intergenic 15.0%
!!! TTAAAAATTTTGTAGTATGA+AGG + chr4.3:37777152-37777171 MS.gene54593:intron 15.0%
!! AATCATAAAGGATTTGAATA+TGG + chr4.3:37777954-37777973 MS.gene54593:CDS 20.0%
!! ACTATATCACAAATGTATAA+GGG - chr4.3:37778049-37778068 None:intergenic 20.0%
!! AGAAGTAATTAAAAATAACC+TGG - chr4.3:37777236-37777255 None:intergenic 20.0%
!! CTAACATTTATATCTAATCA+TGG + chr4.3:37779638-37779657 MS.gene54593:intron 20.0%
!! GAAGTAATTAAAAATAACCT+GGG - chr4.3:37777235-37777254 None:intergenic 20.0%
!! GACTGCATATAATTAAAAAA+AGG - chr4.3:37779817-37779836 None:intergenic 20.0%
!! TAAATCGTTAGATTAAGATA+GGG + chr4.3:37777382-37777401 MS.gene54593:intron 20.0%
!! TACTATATCACAAATGTATA+AGG - chr4.3:37778050-37778069 None:intergenic 20.0%
!! TTAAATCGTTAGATTAAGAT+AGG + chr4.3:37777381-37777400 MS.gene54593:intron 20.0%
!!! AATAGAGTTATTACCATTTT+CGG - chr4.3:37779932-37779951 None:intergenic 20.0%
!!! ATCTAAACTTCATTTGATTT+TGG + chr4.3:37779160-37779179 MS.gene54593:intron 20.0%
!!! ATTTTCGGTAATGTAAATTA+CGG - chr4.3:37779917-37779936 None:intergenic 20.0%
!!! CATGTTTTTTTTGGTAATAT+GGG + chr4.3:37777288-37777307 MS.gene54593:intron 20.0%
!!! GATTTGTTTATGACTATTTA+GGG + chr4.3:37778378-37778397 MS.gene54593:intron 20.0%
!!! TCATGTTTTTTTTGGTAATA+TGG + chr4.3:37777287-37777306 MS.gene54593:intron 20.0%
! AAATGTTGCACTAAATTGAA+TGG - chr4.3:37777541-37777560 None:intergenic 25.0%
! AAGTGACAAAATCAATTGAT+AGG + chr4.3:37780012-37780031 MS.gene54593:intron 25.0%
! ATAATCATTGAGAATAATGC+AGG + chr4.3:37778239-37778258 MS.gene54593:intron 25.0%
! ATAGGTAAATGTAAAAAGGA+AGG - chr4.3:37780236-37780255 None:intergenic 25.0%
! CATCTCAATATGGTATAATA+GGG + chr4.3:37778930-37778949 MS.gene54593:CDS 25.0%
! CTTGAACATAATAAAGATGA+CGG - chr4.3:37780113-37780132 None:intergenic 25.0%
! GATTCTGTAAGTATCTAAAA+GGG - chr4.3:37780079-37780098 None:intergenic 25.0%
! GTAATTTACATTACCGAAAA+TGG + chr4.3:37779916-37779935 MS.gene54593:CDS 25.0%
! TAGATTACTCTATGTATTTC+AGG + chr4.3:37777921-37777940 MS.gene54593:intron 25.0%
! TAGGTAAATGTAAAAAGGAA+GGG - chr4.3:37780235-37780254 None:intergenic 25.0%
! TATGCATAAAGGTCAATAAT+AGG - chr4.3:37780254-37780273 None:intergenic 25.0%
! TATGTATTTCAGGAAGATAT+TGG + chr4.3:37777931-37777950 MS.gene54593:intron 25.0%
! TTACAAAGATATGACTTACT+TGG - chr4.3:37778018-37778037 None:intergenic 25.0%
!! CGATTTGTTTATGACTATTT+AGG + chr4.3:37778377-37778396 MS.gene54593:intron 25.0%
!! CTTTTCCTTAATGATGTATT+TGG + chr4.3:37777620-37777639 MS.gene54593:intron 25.0%
!! TTTTCGGTAATGTAAATTAC+GGG - chr4.3:37779916-37779935 None:intergenic 25.0%
!!! ACCAAAATTTTGAAATATGC+AGG + chr4.3:37778907-37778926 MS.gene54593:intron 25.0%
!!! TACTTATTTTCATATCCATG+TGG + chr4.3:37778203-37778222 MS.gene54593:intron 25.0%
AAAACAGAAGACCTGATATT+TGG - chr4.3:37778705-37778724 None:intergenic 30.0%
AAAATAAGAGATGGAGGTAA+TGG + chr4.3:37777806-37777825 MS.gene54593:CDS 30.0%
AAATATATACCATTGGACCA+AGG - chr4.3:37779705-37779724 None:intergenic 30.0%
AAGAGGAAGAGTAATAGTTT+AGG - chr4.3:37779457-37779476 None:intergenic 30.0%
AATATATACCATTGGACCAA+GGG - chr4.3:37779704-37779723 None:intergenic 30.0%
ACTATACAAGACCAAATATC+AGG + chr4.3:37778691-37778710 MS.gene54593:CDS 30.0%
ACTTGATCTTCTGTAATAGA+AGG + chr4.3:37778790-37778809 MS.gene54593:intron 30.0%
AGCAAAAACTTATGCTATGT+TGG - chr4.3:37777706-37777725 None:intergenic 30.0%
ATATCACAAATGTATAAGGG+AGG - chr4.3:37778046-37778065 None:intergenic 30.0%
ATATTATTTGGACATGTTGC+AGG - chr4.3:37777320-37777339 None:intergenic 30.0%
ATCAATAACTAGAAGCTTAG+AGG - chr4.3:37780170-37780189 None:intergenic 30.0%
ATTGACCTTTATGCATAACA+GGG + chr4.3:37780257-37780276 MS.gene54593:intron 30.0%
ATTGTTGGATAACCATGAAT+GGG + chr4.3:37780314-37780333 MS.gene54593:CDS 30.0%
ATTTATGATTGACAACCACA+TGG - chr4.3:37778221-37778240 None:intergenic 30.0%
CGATTCTGTAAGTATCTAAA+AGG - chr4.3:37780080-37780099 None:intergenic 30.0%
CTTGATCTTCTGTAATAGAA+GGG + chr4.3:37778791-37778810 MS.gene54593:intron 30.0%
CTTGCAGAAAAAATAAGAGA+TGG + chr4.3:37777797-37777816 MS.gene54593:intron 30.0%
GATAAGAGAATCAGTTTGTA+TGG - chr4.3:37779519-37779538 None:intergenic 30.0%
GCATCTCAATATGGTATAAT+AGG + chr4.3:37778929-37778948 MS.gene54593:CDS 30.0%
GGAAGATATTGGAATCATAA+AGG + chr4.3:37777942-37777961 MS.gene54593:intron 30.0%
TATATGGATCCAATCACAAA+AGG + chr4.3:37779332-37779351 MS.gene54593:CDS 30.0%
TATTGACCTTTATGCATAAC+AGG + chr4.3:37780256-37780275 MS.gene54593:intron 30.0%
TCATTTATGCCCTAAATGAA+CGG - chr4.3:37777470-37777489 None:intergenic 30.0%
TCCTATTAACTATTTGCAGA+AGG + chr4.3:37778083-37778102 MS.gene54593:intron 30.0%
TCCTTCTGCAAATAGTTAAT+AGG - chr4.3:37778087-37778106 None:intergenic 30.0%
TGTAGTAGTTTATTCCTTCA+CGG - chr4.3:37778130-37778149 None:intergenic 30.0%
TTCCTATTACTTTGCACAAA+TGG - chr4.3:37779253-37779272 None:intergenic 30.0%
TTGCACCAAATACATCATTA+AGG - chr4.3:37777628-37777647 None:intergenic 30.0%
TTTCGGTAATGTAAATTACG+GGG - chr4.3:37779915-37779934 None:intergenic 30.0%
! AATCGCTCTAGATTGAAAAT+TGG - chr4.3:37778362-37778381 None:intergenic 30.0%
! ATATGTGACTCTTTTCCATT+GGG + chr4.3:37778271-37778290 MS.gene54593:CDS 30.0%
! ATATTTTGCATGGTCATTGT+TGG + chr4.3:37780299-37780318 MS.gene54593:CDS 30.0%
! ATGTAAAGGGATATTTTGCA+TGG + chr4.3:37780289-37780308 MS.gene54593:CDS 30.0%
! CTTATAGATTTTCCATTGCT+TGG + chr4.3:37777980-37777999 MS.gene54593:CDS 30.0%
! GCCTGCATATTTCAAAATTT+TGG - chr4.3:37778911-37778930 None:intergenic 30.0%
! TCATTTAGGGCATAAATGAA+CGG + chr4.3:37777471-37777490 MS.gene54593:intron 30.0%
!! GCTTCTAGTTATTGATTTGT+AGG + chr4.3:37780174-37780193 MS.gene54593:intron 30.0%
!! TCTCGTGTTATTTGAATTTC+AGG - chr4.3:37778771-37778790 None:intergenic 30.0%
!!! GCATTTTGCATTTTTTCTGT+AGG + chr4.3:37779309-37779328 MS.gene54593:intron 30.0%
!!! GCATTTTTTCTGTAGGTATA+TGG + chr4.3:37779316-37779335 MS.gene54593:intron 30.0%
!!! TTTCTTGGCAAGATTTTTTG+AGG - chr4.3:37780437-37780456 None:intergenic 30.0%
AAGAGAATCAGTTTGTATGG+CGG - chr4.3:37779516-37779535 None:intergenic 35.0%
AATATGCAGGCATCTCAATA+TGG + chr4.3:37778920-37778939 MS.gene54593:intron 35.0%
AATTCATGCCCGTTCATTTA+GGG + chr4.3:37777458-37777477 MS.gene54593:intron 35.0%
ACACAAAAGCTACAAATGAG+AGG - chr4.3:37779762-37779781 None:intergenic 35.0%
AGCATGAATGCTTATGCATA+TGG + chr4.3:37778548-37778567 MS.gene54593:CDS 35.0%
ATATATACCATTGGACCAAG+GGG - chr4.3:37779703-37779722 None:intergenic 35.0%
ATGGAAAAGGACCAAGTATT+TGG + chr4.3:37777189-37777208 MS.gene54593:CDS 35.0%
ATTATGTTCAAGCTGCAATC+AGG + chr4.3:37780121-37780140 MS.gene54593:CDS 35.0%
CAATGGAAAAGAGTCACATA+TGG - chr4.3:37778272-37778291 None:intergenic 35.0%
CATTGTTGGATAACCATGAA+TGG + chr4.3:37780313-37780332 MS.gene54593:CDS 35.0%
CATTTATGCCCTAAATGAAC+GGG - chr4.3:37777469-37777488 None:intergenic 35.0%
GCAGAAAAAATAAGAGATGG+AGG + chr4.3:37777800-37777819 MS.gene54593:intron 35.0%
GCATGAATGCTTATGCATAT+GGG + chr4.3:37778549-37778568 MS.gene54593:CDS 35.0%
GGATTACTTTGAATGAACCA+TGG + chr4.3:37778519-37778538 MS.gene54593:CDS 35.0%
GTTTAGGCCAAGAAAATCAA+AGG - chr4.3:37779441-37779460 None:intergenic 35.0%
TGGAAAAGGACCAAGTATTT+GGG + chr4.3:37777190-37777209 MS.gene54593:CDS 35.0%
TGGTTAAATCTGAATTGCAC+AGG + chr4.3:37778599-37778618 MS.gene54593:CDS 35.0%
TTAAACTTAGTGCAACTTGC+TGG + chr4.3:37777597-37777616 MS.gene54593:intron 35.0%
TTAAATCTGAATTGCACAGG+AGG + chr4.3:37778602-37778621 MS.gene54593:CDS 35.0%
! AAAAGTATGCGATCTTTGGT+TGG + chr4.3:37779365-37779384 MS.gene54593:CDS 35.0%
! ACCAAAGATCGCATACTTTT+TGG - chr4.3:37779365-37779384 None:intergenic 35.0%
! ATCAACGAACTATTGGCTAA+TGG + chr4.3:37778158-37778177 MS.gene54593:CDS 35.0%
! ATCTTGCAGAGACGATTTTA+GGG + chr4.3:37778448-37778467 MS.gene54593:intron 35.0%
! CATATGTGACTCTTTTCCAT+TGG + chr4.3:37778270-37778289 MS.gene54593:CDS 35.0%
! GATTCTTCTTTGGAGAACTT+TGG - chr4.3:37779401-37779420 None:intergenic 35.0%
! GGATAGTTACCTTTTGTGAT+TGG - chr4.3:37779344-37779363 None:intergenic 35.0%
! TCCAAAAAGTATGCGATCTT+TGG + chr4.3:37779361-37779380 MS.gene54593:CDS 35.0%
! TGCCATTTGTGCAAAGTAAT+AGG + chr4.3:37779248-37779267 MS.gene54593:intron 35.0%
! TTGGTGATAGAGTGAAACAT+TGG + chr4.3:37778498-37778517 MS.gene54593:CDS 35.0%
!! GTGAAAGTATCCCAAATACT+TGG - chr4.3:37777203-37777222 None:intergenic 35.0%
AACAGCTTCTTCTGCATTTC+AGG + chr4.3:37777006-37777025 MS.gene54593:intron 40.0%
AACATCTTCTTTCGCTTGAG+AGG - chr4.3:37778987-37779006 None:intergenic 40.0%
AAGAATCCGAGGATCTTAAG+GGG + chr4.3:37779414-37779433 MS.gene54593:CDS 40.0%
AAGATCCTCGGATTCTTCTT+TGG - chr4.3:37779411-37779430 None:intergenic 40.0%
ACCTTCCCTGTTATGCATAA+AGG - chr4.3:37780265-37780284 None:intergenic 40.0%
ACCTTTATGCATAACAGGGA+AGG + chr4.3:37780261-37780280 MS.gene54593:intron 40.0%
AGAAGAATCCGAGGATCTTA+AGG + chr4.3:37779412-37779431 MS.gene54593:CDS 40.0%
AGAGAATCAGTTTGTATGGC+GGG - chr4.3:37779515-37779534 None:intergenic 40.0%
AGCTCCTTTCTTTATGCTTG+TGG + chr4.3:37777502-37777521 MS.gene54593:intron 40.0%
ATATGCAGTCTGCTTCAAGT+TGG + chr4.3:37779827-37779846 MS.gene54593:intron 40.0%
CAACCTCATCAACGAACTAT+TGG + chr4.3:37778151-37778170 MS.gene54593:CDS 40.0%
CACCAAATTCCTTGTAGCAA+AGG - chr4.3:37778484-37778503 None:intergenic 40.0%
CTCATACCAATGAGAGAGTA+AGG - chr4.3:37778960-37778979 None:intergenic 40.0%
GAAACGACACCCAAAACTTT+CGG + chr4.3:37780392-37780411 MS.gene54593:CDS 40.0%
GAAGAATCCGAGGATCTTAA+GGG + chr4.3:37779413-37779432 MS.gene54593:CDS 40.0%
GAATTCATGCCCGTTCATTT+AGG + chr4.3:37777457-37777476 MS.gene54593:intron 40.0%
GACCTTTGCTACAAGGAATT+TGG + chr4.3:37778479-37778498 MS.gene54593:CDS 40.0%
GATAACCATGAATGGGAATC+TGG + chr4.3:37780321-37780340 MS.gene54593:CDS 40.0%
GATCCCACAAGCATAAAGAA+AGG - chr4.3:37777509-37777528 None:intergenic 40.0%
GCAGCATTTGAAGATGGAAA+AGG + chr4.3:37777176-37777195 MS.gene54593:CDS 40.0%
GCTCCTTTCTTTATGCTTGT+GGG + chr4.3:37777503-37777522 MS.gene54593:intron 40.0%
GGATTACCTTACTCTCTCAT+TGG + chr4.3:37778951-37778970 MS.gene54593:CDS 40.0%
TAAGTAGCTTCTGAATGCCT+TGG + chr4.3:37777676-37777695 MS.gene54593:intron 40.0%
TAGCCAATAGTTCGTTGATG+AGG - chr4.3:37778157-37778176 None:intergenic 40.0%
TATGCTATGTTGGATCTCCA+AGG - chr4.3:37777696-37777715 None:intergenic 40.0%
TCCATTCATGCTACGTAACT+AGG - chr4.3:37777752-37777771 None:intergenic 40.0%
TCTGTAATAGAAGGGCAAGT+CGG + chr4.3:37778799-37778818 MS.gene54593:intron 40.0%
TTCGGGAACAGAACCATATT+TGG + chr4.3:37778628-37778647 MS.gene54593:CDS 40.0%
TTTGCAGAAGGAAAGCTTAG+TGG + chr4.3:37778095-37778114 MS.gene54593:intron 40.0%
! AACCAATGTGCCGAAAGTTT+TGG - chr4.3:37780405-37780424 None:intergenic 40.0%
! ACCAATGTGCCGAAAGTTTT+GGG - chr4.3:37780404-37780423 None:intergenic 40.0%
! AGGGAAGGTGTGAATGTAAA+GGG + chr4.3:37780276-37780295 MS.gene54593:intron 40.0%
! GATCTTGCAGAGACGATTTT+AGG + chr4.3:37778447-37778466 MS.gene54593:intron 40.0%
! GATTCTTCAAGCGACAGTTT+TGG - chr4.3:37780057-37780076 None:intergenic 40.0%
!! AAGGGGTCCTTTGATTTTCT+TGG + chr4.3:37779431-37779450 MS.gene54593:CDS 40.0%
!! CAAGGTATGTAGTTTTGCAG+AGG + chr4.3:37777859-37777878 MS.gene54593:intron 40.0%
!!! ATGACGGTTTTTTAAGCCCT+CGG + chr4.3:37778321-37778340 MS.gene54593:CDS 40.0%
AAAGGACCCCTTAAGATCCT+CGG - chr4.3:37779423-37779442 None:intergenic 45.0%
AAATCGTCTCTGCAAGATCG+CGG - chr4.3:37778446-37778465 None:intergenic 45.0%
AACAGTTGTCCTAATCCTCG+CGG - chr4.3:37779865-37779884 None:intergenic 45.0%
AACCAATCTGAACATCGACC+CGG - chr4.3:37778584-37778603 None:intergenic 45.0%
AATTGCACAGGAGGTGATTC+GGG + chr4.3:37778611-37778630 MS.gene54593:CDS 45.0%
ACCATCCAAACATGAAGTCG+AGG - chr4.3:37779027-37779046 None:intergenic 45.0%
ACCCAAAACTTTCGGCACAT+TGG + chr4.3:37780400-37780419 MS.gene54593:CDS 45.0%
ACCGTCATACTCATCCTCTA+AGG - chr4.3:37778309-37778328 None:intergenic 45.0%
ACTCTCTCATTGGTATGAGC+CGG + chr4.3:37778961-37778980 MS.gene54593:CDS 45.0%
ACTTTCACTCATGAACACCC+AGG + chr4.3:37777215-37777234 MS.gene54593:CDS 45.0%
CGAAGATGGAGTAGTGAAGA+AGG - chr4.3:37776935-37776954 None:intergenic 45.0%
CTATGCTGACCTTTGCTACA+AGG + chr4.3:37778472-37778491 MS.gene54593:CDS 45.0%
GAAACAGGTCCGATTGAGAT+CGG - chr4.3:37776966-37776985 None:intergenic 45.0%
GAATTGCACAGGAGGTGATT+CGG + chr4.3:37778610-37778629 MS.gene54593:CDS 45.0%
GCCTAGTTACGTAGCATGAA+TGG + chr4.3:37777748-37777767 MS.gene54593:intron 45.0%
GCCTTAGAGGATGAGTATGA+CGG + chr4.3:37778305-37778324 MS.gene54593:CDS 45.0%
GGCATAAATGAACGGACATG+TGG + chr4.3:37777479-37777498 MS.gene54593:intron 45.0%
GTTCTCCAAAGAAGAATCCG+AGG + chr4.3:37779403-37779422 MS.gene54593:CDS 45.0%
TAGCACTCTAGACCAAGCAA+TGG - chr4.3:37777995-37778014 None:intergenic 45.0%
TCGATGACAGTTCATCGTGA+TGG + chr4.3:37779673-37779692 MS.gene54593:intron 45.0%
TGAAGCCAGATTCCCATTCA+TGG - chr4.3:37780329-37780348 None:intergenic 45.0%
TGCATATGGGAAGTTTGCAC+CGG + chr4.3:37778562-37778581 MS.gene54593:CDS 45.0%
TGCATTTATCCGCGAGGATT+AGG + chr4.3:37779853-37779872 MS.gene54593:CDS 45.0%
TGGTTATGCATTTATCCGCG+AGG + chr4.3:37779847-37779866 MS.gene54593:CDS 45.0%
! CAGGGAAGGTGTGAATGTAA+AGG + chr4.3:37780275-37780294 MS.gene54593:intron 45.0%
! CTTTGGAGAACTTTGGTAGC+CGG - chr4.3:37779394-37779413 None:intergenic 45.0%
! GAAGGTGCAGCATTTGAAGA+TGG + chr4.3:37777170-37777189 MS.gene54593:CDS 45.0%
! GCGACTTTTCCGATCTCAAT+CGG + chr4.3:37776954-37776973 MS.gene54593:CDS 45.0%
! GCTTTACGATGCAAGTTGGA+CGG - chr4.3:37777427-37777446 None:intergenic 45.0%
! TGGTAGTGTGCAGCCAAATA+TGG - chr4.3:37778644-37778663 None:intergenic 45.0%
!! GTTGGCTTTACGATGCAAGT+TGG - chr4.3:37777431-37777450 None:intergenic 45.0%
!! TACTCATCCTCTAAGGCTTG+AGG - chr4.3:37778302-37778321 None:intergenic 45.0%
!!! ATATTTTAGAAATAATATTT+TGG - chr4.3:37779786-37779805 None:intergenic 5.0%
AGGTGCATCAGCAGCATAAG+AGG - chr4.3:37779474-37779493 None:intergenic 50.0%
CAGCAGCATGAGCAAGAAGT+TGG - chr4.3:37778664-37778683 None:intergenic 50.0%
CGACGCATATCATCGGTACA+AGG + chr4.3:37777841-37777860 MS.gene54593:CDS 50.0%
GATCGGAAAAGTCGCGAAGA+TGG - chr4.3:37776949-37776968 None:intergenic 50.0%
GATGTTGATGCTGCACAACG+AGG + chr4.3:37779001-37779020 MS.gene54593:CDS 50.0%
GCAGAAGGAAAGCTTAGTGG+AGG + chr4.3:37778098-37778117 MS.gene54593:intron 50.0%
GCATATGGGAAGTTTGCACC+GGG + chr4.3:37778563-37778582 MS.gene54593:CDS 50.0%
GCATTCATGCTCACACTCCA+TGG - chr4.3:37778539-37778558 None:intergenic 50.0%
GCCTCGACTTCATGTTTGGA+TGG + chr4.3:37779023-37779042 MS.gene54593:CDS 50.0%
GCTGTTCCGAAAACAAAGCC+TGG - chr4.3:37776992-37777011 None:intergenic 50.0%
GGGCATGAATTCGCTACAGT+TGG - chr4.3:37777449-37777468 None:intergenic 50.0%
GTTCCGAAAACAAAGCCTGG+CGG - chr4.3:37776989-37777008 None:intergenic 50.0%
TAGCCGACGACGCATATCAT+CGG + chr4.3:37777834-37777853 MS.gene54593:CDS 50.0%
TCTTCTTTCGCTTGAGAGGC+CGG - chr4.3:37778983-37779002 None:intergenic 50.0%
TGCGATCTTTGGTTGGAAGC+CGG + chr4.3:37779372-37779391 MS.gene54593:CDS 50.0%
TTCCGAAAACAAAGCCTGGC+GGG - chr4.3:37776988-37777007 None:intergenic 50.0%
! AGTGGAGGTGTAAACCGTGA+AGG + chr4.3:37778113-37778132 MS.gene54593:CDS 50.0%
! GCGATTTGGACTCGTCTTTG+TGG + chr4.3:37780353-37780372 MS.gene54593:CDS 50.0%
! GTACCGATGATATGCGTCGT+CGG - chr4.3:37777840-37777859 None:intergenic 50.0%
! GTGTCACTCACACAATCCGA+GGG - chr4.3:37778340-37778359 None:intergenic 50.0%
! TCTGGCTTCAGCTTGCGATT+TGG + chr4.3:37780339-37780358 MS.gene54593:CDS 50.0%
!! ATCAGTTTGTATGGCGGGTC+TGG - chr4.3:37779510-37779529 None:intergenic 50.0%
!!! TTCCCGCCAGGCTTTGTTTT+CGG + chr4.3:37776983-37777002 MS.gene54593:intron 50.0%
AACAAAGCCTGGCGGGAAAC+AGG - chr4.3:37776981-37777000 None:intergenic 55.0%
AGGCTTGAGGAACGTCCCAA+TGG - chr4.3:37778289-37778308 None:intergenic 55.0%
CACCGGGTCGATGTTCAGAT+TGG + chr4.3:37778579-37778598 MS.gene54593:CDS 55.0%
CGAGGCCTCGACTTCATGTT+TGG + chr4.3:37779019-37779038 MS.gene54593:CDS 55.0%
GGACGTTCCTCAAGCCTTAG+AGG + chr4.3:37778292-37778311 MS.gene54593:CDS 55.0%
GGTCTGGCATCACGTAAGTG+AGG - chr4.3:37779494-37779513 None:intergenic 55.0%
!! GGTGTCACTCACACAATCCG+AGG - chr4.3:37778341-37778360 None:intergenic 55.0%
AATCGGACCTGTTTCCCGCC+AGG + chr4.3:37776971-37776990 MS.gene54593:intron 60.0%
CATCGTGATGGCAAGCCCCT+TGG + chr4.3:37779685-37779704 MS.gene54593:CDS 60.0%
TGGCAAGCCCCTTGGTCCAA+TGG + chr4.3:37779693-37779712 MS.gene54593:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 37776921 37780470 37776921 ID=MS.gene54593
chr4.3 mRNA 37776921 37780470 37776921 ID=MS.gene54593.t1;Parent=MS.gene54593
chr4.3 exon 37776921 37776980 37776921 ID=MS.gene54593.t1.exon1;Parent=MS.gene54593.t1
chr4.3 CDS 37776921 37776980 37776921 ID=cds.MS.gene54593.t1;Parent=MS.gene54593.t1
chr4.3 exon 37777167 37777236 37777167 ID=MS.gene54593.t1.exon2;Parent=MS.gene54593.t1
chr4.3 CDS 37777167 37777236 37777167 ID=cds.MS.gene54593.t1;Parent=MS.gene54593.t1
chr4.3 exon 37777804 37777862 37777804 ID=MS.gene54593.t1.exon3;Parent=MS.gene54593.t1
chr4.3 CDS 37777804 37777862 37777804 ID=cds.MS.gene54593.t1;Parent=MS.gene54593.t1
chr4.3 exon 37777943 37778018 37777943 ID=MS.gene54593.t1.exon4;Parent=MS.gene54593.t1
chr4.3 CDS 37777943 37778018 37777943 ID=cds.MS.gene54593.t1;Parent=MS.gene54593.t1
chr4.3 exon 37778102 37778179 37778102 ID=MS.gene54593.t1.exon5;Parent=MS.gene54593.t1
chr4.3 CDS 37778102 37778179 37778102 ID=cds.MS.gene54593.t1;Parent=MS.gene54593.t1
chr4.3 exon 37778261 37778348 37778261 ID=MS.gene54593.t1.exon6;Parent=MS.gene54593.t1
chr4.3 CDS 37778261 37778348 37778261 ID=cds.MS.gene54593.t1;Parent=MS.gene54593.t1
chr4.3 exon 37778457 37778712 37778457 ID=MS.gene54593.t1.exon7;Parent=MS.gene54593.t1
chr4.3 CDS 37778457 37778712 37778457 ID=cds.MS.gene54593.t1;Parent=MS.gene54593.t1
chr4.3 exon 37778929 37779044 37778929 ID=MS.gene54593.t1.exon8;Parent=MS.gene54593.t1
chr4.3 CDS 37778929 37779044 37778929 ID=cds.MS.gene54593.t1;Parent=MS.gene54593.t1
chr4.3 exon 37779331 37779548 37779331 ID=MS.gene54593.t1.exon9;Parent=MS.gene54593.t1
chr4.3 CDS 37779331 37779548 37779331 ID=cds.MS.gene54593.t1;Parent=MS.gene54593.t1
chr4.3 exon 37779683 37779714 37779683 ID=MS.gene54593.t1.exon10;Parent=MS.gene54593.t1
chr4.3 CDS 37779683 37779714 37779683 ID=cds.MS.gene54593.t1;Parent=MS.gene54593.t1
chr4.3 exon 37779835 37779937 37779835 ID=MS.gene54593.t1.exon11;Parent=MS.gene54593.t1
chr4.3 CDS 37779835 37779937 37779835 ID=cds.MS.gene54593.t1;Parent=MS.gene54593.t1
chr4.3 exon 37780034 37780142 37780034 ID=MS.gene54593.t1.exon12;Parent=MS.gene54593.t1
chr4.3 CDS 37780034 37780142 37780034 ID=cds.MS.gene54593.t1;Parent=MS.gene54593.t1
chr4.3 exon 37780278 37780470 37780278 ID=MS.gene54593.t1.exon13;Parent=MS.gene54593.t1
chr4.3 CDS 37780278 37780470 37780278 ID=cds.MS.gene54593.t1;Parent=MS.gene54593.t1
Gene Sequence

>MS.gene54593

ATGCTTTTGAACCTTCTTCACTACTCCATCTTCGCGACTTTTCCGATCTCAATCGGACCTGTTTCCCGCCAGGCTTTGTTTTCGGAACAGCTTCTTCTGCATTTCAGGCACACAAACAAACATCTTTCAATTTTATTATTATTATTATTATTATTATTTATAACATGCATGCAATGTAGAAATGTAGTTATTAGATATTTGATTTTGTGTAATGATTAAGTTTCATGTTATTTAAAAATTTTGTAGTATGAAGGTGCAGCATTTGAAGATGGAAAAGGACCAAGTATTTGGGATACTTTCACTCATGAACACCCAGGTTATTTTTAATTACTTCTCTTTCATTTTGCAATTTTGAAAATATTAATATCATGTTTTTTTTGGTAATATGGGTTCAAACCTGCAACATGTCCAAATAATATTAGTATGTTATTTTACATCACGCTTTATTGCACATCTGATCTTAAATCGTTAGATTAAGATAGGGAAACTCTATCTCAATATAACCGTCCAACTTGCATCGTAAAGCCAACTGTAGCGAATTCATGCCCGTTCATTTAGGGCATAAATGAACGGACATGTGGAGCTCCTTTCTTTATGCTTGTGGGATCTTGCATGTTCCATTCAATTTAGTGCAACATTTGCCTTTTCAAAAAAAAAAAAATTGTAATAATAATAATTAAACTTAGTGCAACTTGCTGGCTTTTCCTTAATGATGTATTTGGTGCAAGAATCATGATCAACAGTCATTTTCAGCATAAGTAGCTTCTGAATGCCTTGGAGATCCAACATAGCATAAGTTTTTGCTTGCTTTACTTATATATTGATTTGCCTAGTTACGTAGCATGAATGGATTTCTCATATATATTAATGATATGGCTTGCAGAAAAAATAAGAGATGGAGGTAATGGAGATGTAGCCGACGACGCATATCATCGGTACAAGGTATGTAGTTTTGCAGAGGTATATAAAATACAAATGTAACATAGATTGAGACTCATGATAGATTACTCTATGTATTTCAGGAAGATATTGGAATCATAAAGGATTTGAATATGGATGCTTATAGATTTTCCATTGCTTGGTCTAGAGTGCTACCAAGTAAGTCATATCTTTGTAAATATTCCTCCCTTATACATTTGTGATATAGTAATCATCATAAATTTCCTATTAACTATTTGCAGAAGGAAAGCTTAGTGGAGGTGTAAACCGTGAAGGAATAAACTACTACAACAACCTCATCAACGAACTATTGGCTAATGGTAAAGATTCATCAATTTTTATTTACTTATTTTCATATCCATGTGGTTGTCAATCATAAATAATCATTGAGAATAATGCAGGTTTGCAGCCATATGTGACTCTTTTCCATTGGGACGTTCCTCAAGCCTTAGAGGATGAGTATGACGGTTTTTTAAGCCCTCGGATTGTGTGAGTGACACCAATTTTCAATCTAGAGCGATTTGTTTATGACTATTTAGGGAAATATTGCAGTAAATAAAATTAGTCGATTAACATATGTAATCCGCGATCTTGCAGAGACGATTTTAGGGACTATGCTGACCTTTGCTACAAGGAATTTGGTGATAGAGTGAAACATTGGATTACTTTGAATGAACCATGGAGTGTGAGCATGAATGCTTATGCATATGGGAAGTTTGCACCGGGTCGATGTTCAGATTGGTTAAATCTGAATTGCACAGGAGGTGATTCGGGAACAGAACCATATTTGGCTGCACACTACCAACTTCTTGCTCATGCTGCTGCTACAAAACTATACAAGACCAAATATCAGGTCTTCTGTTTTTAAAAGCAATATATTATTCAAATTCGGCACTGAATCTTTATGTCCTGAAATTCAAATAACACGAGACTTGATCTTCTGTAATAGAAGGGCAAGTCGGACAAAAGTATACACAATTGCTTTCTATTGATAGATCATACTTATTTTTTTAGTGTGAACTTATTAGAGCTGTTTCTAACCAAATAACCAAAATTTTGAAATATGCAGGCATCTCAATATGGTATAATAGGGATTACCTTACTCTCTCATTGGTATGAGCCGGCCTCTCAAGCGAAAGAAGATGTTGATGCTGCACAACGAGGCCTCGACTTCATGTTTGGATGGTAAAGATTAATTTTAACTATTCGTAGCTCAACTAACAGATATGATCATGAAATTTTTTGAAAATATTAATTAATTTTTCTAACAATGTGATCATGATAATCTGATTAATATTGAATCTAAACTTCATTTGATTTTGGTTCACTTTGTGTTTTGAACTCAAGAAATTAGTTATGTAGATTTGCTGAATTCAAATGCAACTATATGCCATTTGTGCAAAGTAATAGGAAATCAAAGAAGACTAATACATTTTTGTGATTGCATCTGCATTTTGCATTTTTTCTGTAGGTATATGGATCCAATCACAAAAGGTAACTATCCAAAAAGTATGCGATCTTTGGTTGGAAGCCGGCTACCAAAGTTCTCCAAAGAAGAATCCGAGGATCTTAAGGGGTCCTTTGATTTTCTTGGCCTAAACTATTACTCTTCCTCTTATGCTGCTGATGCACCTCACTTACGTGATGCCAGACCCGCCATACAAACTGATTCTCTTATCAATGCTACATGTAATTATCAAATTAAATATATGCTTGCACTAGTTAATTGTTTTGAACTGAAGTGCAACAGAATTGAAAGAAAATTCATCATACATACTCTAACATTTATATCTAATCATGGTTTTTTATTTTTTCGATGACAGTTCATCGTGATGGCAAGCCCCTTGGTCCAATGGTATATATTTTATCATTCATTAGCATCTAATTTGCATATCAATGCCTCTCATTTGTAGCTTTTGTGTTCCAAAATATTATTTCTAAAATATAACGTGAACCTTTTTTTAATTATATGCAGTCTGCTTCAAGTTGGTTATGCATTTATCCGCGAGGATTAGGACAACTGTTGCTTTACGTCAAGAATAAGTATAACAACCCCGTAATTTACATTACCGAAAATGGTAATAACTCTATTATACTTCTTATTCTCTTTTATATTATATGCATAATACTGAAACTTTAATAAAGCTAATGAAAGTGACAAAATCAATTGATAGGTCGCGATGAGTTCAACGATCCAAAACTGTCGCTTGAAGAATCCCTTTTAGATACTTACAGAATCGATTACTTTTACCGTCATCTTTATTATGTTCAAGCTGCAATCAGGTAACTAATGCTTTATCAGAAGAGCCTCTAAGCTTCTAGTTATTGATTTGTAGGTTTTATACATATCAATTGACAATGCATTTTTTCTACCCTTCCTTTTTACATTTACCTATTATTGACCTTTATGCATAACAGGGAAGGTGTGAATGTAAAGGGATATTTTGCATGGTCATTGTTGGATAACCATGAATGGGAATCTGGCTTCAGCTTGCGATTTGGACTCGTCTTTGTGGATTATAACGACGACTTGAAACGACACCCAAAACTTTCGGCACATTGGTTCAAGAATTTCCTCAAAAAATCTTGCCAAGAAAAGTGTTCTGTTTAA
Protein sequence

>MS.gene54593.t1

MLLNLLHYSIFATFPISIGPYEGAAFEDGKGPSIWDTFTHEHPEKIRDGGNGDVADDAYHRYKEDIGIIKDLNMDAYRFSIAWSRVLPKGKLSGGVNREGINYYNNLINELLANGLQPYVTLFHWDVPQALEDEYDGFLSPRIVDDFRDYADLCYKEFGDRVKHWITLNEPWSVSMNAYAYGKFAPGRCSDWLNLNCTGGDSGTEPYLAAHYQLLAHAAATKLYKTKYQASQYGIIGITLLSHWYEPASQAKEDVDAAQRGLDFMFGWYMDPITKGNYPKSMRSLVGSRLPKFSKEESEDLKGSFDFLGLNYYSSSYAADAPHLRDARPAIQTDSLINATFHRDGKPLGPMSASSWLCIYPRGLGQLLLYVKNKYNNPVIYITENGRDEFNDPKLSLEESLLDTYRIDYFYRHLYYVQAAIREGVNVKGYFAWSLLDNHEWESGFSLRFGLVFVDYNDDLKRHPKLSAHWFKNFLKKSCQEKCSV