AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54739

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54739.t1 MTR_6g008950 92.405 316 21 1 1 316 1 313 0.0 588
MS.gene54739.t1 MTR_4g107090 31.489 235 114 7 14 210 22 247 1.26e-15 75.5
MS.gene54739.t1 MTR_2g093100 45.283 53 23 1 243 289 9 61 7.97e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54739.t1 AT5G62910 37.821 312 145 9 5 290 11 299 2.97e-41 146
MS.gene54739.t1 AT5G62910 37.821 312 145 9 5 290 11 299 2.97e-41 146
MS.gene54739.t1 AT3G48070 40.206 291 139 11 14 290 22 291 1.60e-40 144
MS.gene54739.t1 AT3G48070 40.206 291 139 11 14 290 22 291 9.18e-40 143
MS.gene54739.t1 AT3G48070 54.167 96 34 1 194 289 156 241 4.13e-30 116
MS.gene54739.t1 AT1G74870 29.749 279 152 8 13 288 20 257 9.44e-20 87.8
MS.gene54739.t1 AT2G28540 45.283 53 23 1 243 289 9 61 8.95e-11 63.5
MS.gene54739.t1 AT5G60170 45.283 53 23 1 243 289 9 61 9.51e-11 63.2
MS.gene54739.t1 AT5G60170 45.283 53 23 1 243 289 9 61 9.59e-11 63.2
MS.gene54739.t1 AT2G28540 45.283 53 23 1 243 289 9 61 9.93e-11 63.2

Find 74 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAATGGAGTTGTTTATTA+TGG 0.175343 6.2:+4489260 None:intergenic
AGGGTTTACCCTCTTCTTCT+TGG 0.319817 6.2:+4490926 None:intergenic
TTCCTTCATTCAATGGCTCC+TGG 0.362277 6.2:-4490975 None:intergenic
CTCCTGATTTCCTTGTATGA+TGG 0.367192 6.2:+4489330 None:intergenic
TGCATCCAAGCGAATGATTC+CGG 0.369607 6.2:-4489285 MS.gene54739:CDS
AAACTATATGATCATGTAGA+TGG 0.388857 6.2:-4488676 MS.gene54739:CDS
TAATTAATCGTATGCTCTAT+AGG 0.391969 6.2:+4489063 None:intergenic
AAGACGGATGCCTTGATGAT+TGG 0.392511 6.2:-4489146 MS.gene54739:CDS
AACCATCATACAAGGAAATC+AGG 0.402516 6.2:-4489332 MS.gene54739:CDS
CGAGGCAGATGGACGATGTC+CGG 0.409531 6.2:-4488707 MS.gene54739:CDS
AGACGGATGCCTTGATGATT+GGG 0.410627 6.2:-4489145 MS.gene54739:CDS
GGATTTAGACTTGACAGATT+CGG 0.412182 6.2:-4488788 MS.gene54739:CDS
GGATGCGATGTTGACTCAAC+TGG 0.420478 6.2:-4489405 MS.gene54739:CDS
GGAGTTGTTTATTATGGATC+CGG 0.422445 6.2:+4489266 None:intergenic
ACTCTCAGTTAAATTCAAAC+TGG 0.432784 6.2:-4488888 MS.gene54739:CDS
GGACTTAACGATGGACTTAA+CGG 0.443841 6.2:-4489019 MS.gene54739:CDS
CTCTTCTTCTTGGAGGAGAA+AGG 0.450083 6.2:+4490936 None:intergenic
GTGATTATAACTACTTTGAA+TGG 0.452304 6.2:+4489245 None:intergenic
TACAAGGAAATCAGGAGAAA+AGG 0.453486 6.2:-4489324 MS.gene54739:CDS
CAGTCATGCCGTATGAGTAC+AGG 0.456682 6.2:-4488610 MS.gene54739:CDS
TCTAAATCCTCGTAACAGAT+AGG 0.467714 6.2:+4488802 None:intergenic
GGAGAAAGGAGGGTCAGAAA+TGG 0.470846 6.2:+4490950 None:intergenic
GTTTACCCTCTTCTTCTTGG+AGG 0.476527 6.2:+4490929 None:intergenic
ACTTAACGGTGAAAGAGTTC+AGG 0.480803 6.2:-4489005 MS.gene54739:CDS
TTCCTCACTTAGACTTCCTA+AGG 0.483318 6.2:+4489167 None:intergenic
CTCGCAATGTGCTTAGATGA+TGG 0.492888 6.2:+4489366 None:intergenic
CCGTATGAGTACAGGGTGCT+AGG 0.509540 6.2:-4488602 None:intergenic
TGGATCCGGAATCATTCGCT+TGG 0.519416 6.2:+4489280 None:intergenic
CACATATGAGCTGTCGAGCA+TGG 0.522121 6.2:-4488966 MS.gene54739:CDS
GAATTTCTTCCTTGTTCGTG+TGG 0.522525 6.2:-4488766 MS.gene54739:CDS
TGTTGATTCAGAAGCAGCTA+AGG 0.529153 6.2:-4489040 MS.gene54739:CDS
GAGGCAGATGGACGATGTCC+GGG 0.536233 6.2:-4488706 MS.gene54739:CDS
ACTGTTTGCGTTTAAAGCAT+CGG 0.537816 6.2:+4489113 None:intergenic
TATAGGAGACTCTGAAACCA+TGG 0.540448 6.2:+4489080 None:intergenic
TTATGCGGCTTTGAGAGGTC+GGG 0.543577 6.2:+4488910 None:intergenic
TGCGTTTAAAGCATCGGCAA+CGG 0.544753 6.2:+4489119 None:intergenic
AATCAGGAGAAAAGGAAGAG+AGG 0.545531 6.2:-4489316 MS.gene54739:CDS
GTTATGCGGCTTTGAGAGGT+CGG 0.547395 6.2:+4488909 None:intergenic
AGGAGAAAAGGAAGAGAGGA+TGG 0.550600 6.2:-4489312 MS.gene54739:CDS
CTCGGACGAAGAGTGTCATC+GGG 0.558563 6.2:+4488940 None:intergenic
CCTCGGACGAAGAGTGTCAT+CGG 0.565455 6.2:+4488939 None:intergenic
ACAAAATTGTACCTTGGTCA+TGG 0.567148 6.2:-4488855 MS.gene54739:CDS
ATCTAAGCACATTGCGAGCG+AGG 0.569786 6.2:-4489361 MS.gene54739:CDS
TTCTTCTTGGAGGAGAAAGG+AGG 0.571919 6.2:+4490939 None:intergenic
GGAGGGTCAGAAATGGAACC+AGG 0.572536 6.2:+4490957 None:intergenic
CTTTCTCCTCCAAGAAGAAG+AGG 0.573316 6.2:-4490935 MS.gene54739:CDS
CAACGGCCTCCCAATCATCA+AGG 0.573927 6.2:+4489136 None:intergenic
GAGAGTTATGCGGCTTTGAG+AGG 0.580285 6.2:+4488905 None:intergenic
CAGTTAAATTCAAACTGGCA+TGG 0.583633 6.2:-4488883 MS.gene54739:CDS
CCTAGCACCCTGTACTCATA+CGG 0.584262 6.2:+4488602 None:intergenic
AAACAGTAACGAGCATTCCA+TGG 0.585443 6.2:-4489097 MS.gene54739:CDS
AGCAAATTAAGCTTGACGTT+AGG 0.588067 6.2:-4490458 MS.gene54739:CDS
GTTATAATCACGATGAGTCA+AGG 0.589115 6.2:-4489233 MS.gene54739:CDS
AGATGAAATCCACACGAACA+AGG 0.589677 6.2:+4488757 None:intergenic
AATCACGATGAGTCAAGGAA+TGG 0.591635 6.2:-4489228 MS.gene54739:CDS
GTAACCACAAAATTGTACCT+TGG 0.603235 6.2:-4488861 MS.gene54739:CDS
GGAGAATCAACCATCATACA+AGG 0.605659 6.2:-4489340 MS.gene54739:CDS
CGCGAACAATGGCTTTCTAG+AGG 0.607394 6.2:-4490435 MS.gene54739:intron
GCGATGTTGACTCAACTGGA+AGG 0.610065 6.2:-4489401 MS.gene54739:CDS
TCTTCTTGGAGGAGAAAGGA+GGG 0.611617 6.2:+4490940 None:intergenic
AGTCATGCCGTATGAGTACA+GGG 0.616497 6.2:-4488609 MS.gene54739:CDS
ATTATTGTTCGCCATGACCA+AGG 0.622571 6.2:+4488844 None:intergenic
TTGACGTTAGGCGCGAACAA+TGG 0.623569 6.2:-4490446 MS.gene54739:CDS
TTTCTCCTCCAAGAAGAAGA+GGG 0.628934 6.2:-4490934 MS.gene54739:intron
GAATTTAACTGAGAGTTATG+CGG 0.630591 6.2:+4488895 None:intergenic
GGAAGTCTAAGTGAGGAAGA+CGG 0.638004 6.2:-4489162 MS.gene54739:CDS
GAGAGGTCGGGCAAACACCT+CGG 0.641706 6.2:+4488922 None:intergenic
TTCCTTAGGAAGTCTAAGTG+AGG 0.644046 6.2:-4489169 MS.gene54739:CDS
CGGATGCCTTGATGATTGGG+AGG 0.644265 6.2:-4489142 MS.gene54739:CDS
AGCAGCTAAGGACTTAACGA+TGG 0.647033 6.2:-4489028 MS.gene54739:CDS
CCGATGACACTCTTCGTCCG+AGG 0.652440 6.2:-4488939 MS.gene54739:CDS
TAACAGATAGGACATTGACT+AGG 0.694474 6.2:+4488814 None:intergenic
CGATGTTGACTCAACTGGAA+GGG 0.705315 6.2:-4489400 MS.gene54739:CDS
TCAATGTCCTATCTGTTACG+AGG 0.710111 6.2:-4488809 MS.gene54739:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAAAAAAATTCAAAGA+TGG - chr6.2:4490033-4490052 MS.gene54739:intron 10.0%
!! AAAAAGAAAAATCTTATCAT+TGG + chr6.2:4489228-4489247 None:intergenic 15.0%
!! AATAAAAATTAAAAAGGAGA+AGG + chr6.2:4488663-4488682 None:intergenic 15.0%
!! ATAAAAATTAAAAAGGAGAA+GGG + chr6.2:4488662-4488681 None:intergenic 15.0%
!! TTAAAAAATATAATAGCGAA+TGG + chr6.2:4489394-4489413 None:intergenic 15.0%
!!! TTAAATATATTGTTTCTCAA+TGG - chr6.2:4489981-4490000 MS.gene54739:intron 15.0%
!! AACATAATGAATGTCATAAA+AGG + chr6.2:4488958-4488977 None:intergenic 20.0%
!!! AATCAAATTTTTATAGCTCA+AGG + chr6.2:4488719-4488738 None:intergenic 20.0%
!!! AATCTTCATTTTTTATGTCA+TGG - chr6.2:4488884-4488903 MS.gene54739:CDS 20.0%
!!! ATCAAATTTTTATAGCTCAA+GGG + chr6.2:4488718-4488737 None:intergenic 20.0%
!!! TCATTCTGTTATTTATGTAA+GGG - chr6.2:4489288-4489307 MS.gene54739:CDS 20.0%
!!! TTATGATCATCATTTTCATA+TGG - chr6.2:4489159-4489178 MS.gene54739:CDS 20.0%
!!! TTTTGTTTTTCAGTCAAAAA+GGG - chr6.2:4490140-4490159 MS.gene54739:intron 20.0%
! AAACTATATGATCATGTAGA+TGG - chr6.2:4490890-4490909 MS.gene54739:intron 25.0%
! AAATGCAATGAAATGGAAAA+TGG + chr6.2:4488800-4488819 None:intergenic 25.0%
! AATGCAATGAAATGGAAAAT+GGG + chr6.2:4488799-4488818 None:intergenic 25.0%
! CAAAACAAAATGCAATGAAA+TGG + chr6.2:4488807-4488826 None:intergenic 25.0%
! CATTATCTACCAAAATGATT+AGG - chr6.2:4489437-4489456 MS.gene54739:intron 25.0%
! GTGATTATAACTACTTTGAA+TGG + chr6.2:4490324-4490343 None:intergenic 25.0%
! TAATTAATCGTATGCTCTAT+AGG + chr6.2:4490506-4490525 None:intergenic 25.0%
! TTATACTGTTTATCCAAACA+AGG - chr6.2:4489857-4489876 MS.gene54739:intron 25.0%
! TTATTTATGTAAGGGTTTAG+AGG - chr6.2:4489296-4489315 MS.gene54739:CDS 25.0%
! TTCAAGATTCATGATTATGA+AGG + chr6.2:4489266-4489285 None:intergenic 25.0%
! TTGAATGGAGTTGTTTATTA+TGG + chr6.2:4490309-4490328 None:intergenic 25.0%
!! AAATCTTTTTCTCACTAGAT+GGG - chr6.2:4489899-4489918 MS.gene54739:intron 25.0%
!! GTCATTCTGTTATTTATGTA+AGG - chr6.2:4489287-4489306 MS.gene54739:CDS 25.0%
!! TGGAAATTTTCATTATGTCA+TGG - chr6.2:4489026-4489045 MS.gene54739:CDS 25.0%
!! TTCATTATGTTTTTCCTCTA+AGG - chr6.2:4488967-4488986 MS.gene54739:CDS 25.0%
!! TTTTCATTATGTCATGGTTT+TGG - chr6.2:4489032-4489051 MS.gene54739:CDS 25.0%
!!! ATGTCATGGTTTTATCATAA+AGG - chr6.2:4488898-4488917 MS.gene54739:CDS 25.0%
!!! ATTCCGAATTTTAGGTTTTA+GGG - chr6.2:4489624-4489643 MS.gene54739:intron 25.0%
!!! ATTGTGTTTTTTCAGACAAA+TGG - chr6.2:4489071-4489090 MS.gene54739:CDS 25.0%
!!! CTTAAAAATCCTTTTGAAGT+CGG - chr6.2:4490078-4490097 MS.gene54739:intron 25.0%
!!! CTTTTGTTTTTCAGTCAAAA+AGG - chr6.2:4490139-4490158 MS.gene54739:intron 25.0%
!!! TATCATTGATTCCGAATTTT+AGG - chr6.2:4489616-4489635 MS.gene54739:intron 25.0%
!!! TTGCTTTGTTTTTGATGTTT+TGG - chr6.2:4489006-4489025 MS.gene54739:CDS 25.0%
!!! TTTCTATGTTTTGTTGTTCT+TGG - chr6.2:4488859-4488878 MS.gene54739:CDS 25.0%
!!! TTTGTTTTGTTGAGTTCAAA+GGG - chr6.2:4488818-4488837 MS.gene54739:CDS 25.0%
!!! TTTTGTTTTGTTGAGTTCAA+AGG - chr6.2:4488817-4488836 MS.gene54739:CDS 25.0%
AAGAAAAATTGCTTCCTTAG+AGG + chr6.2:4488984-4489003 None:intergenic 30.0%
ACTCTCAGTTAAATTCAAAC+TGG - chr6.2:4490678-4490697 MS.gene54739:intron 30.0%
GAATTTAACTGAGAGTTATG+CGG + chr6.2:4490674-4490693 None:intergenic 30.0%
TATAATAGCGAATGGATCAA+AGG + chr6.2:4489386-4489405 None:intergenic 30.0%
TTATGAAGGCAAGATTATGA+AGG + chr6.2:4489252-4489271 None:intergenic 30.0%
! AAGCTTAGTTTAGTTAGTCT+TGG - chr6.2:4489540-4489559 MS.gene54739:intron 30.0%
! CAAATCTTTTTCTCACTAGA+TGG - chr6.2:4489898-4489917 MS.gene54739:intron 30.0%
! CGATCTTTTTGTGACAAAAA+AGG + chr6.2:4490838-4490857 None:intergenic 30.0%
! GATTAAAGGCCTAATCATTT+TGG + chr6.2:4489449-4489468 None:intergenic 30.0%
! GATTGACTTAAGAGAGTTTA+TGG - chr6.2:4489655-4489674 MS.gene54739:intron 30.0%
!! AAAAAGTTACCGACTTCAAA+AGG + chr6.2:4490090-4490109 None:intergenic 30.0%
!! AGCTTAGTTTAGTTAGTCTT+GGG - chr6.2:4489541-4489560 MS.gene54739:intron 30.0%
!! TCATTTTGGTAGATAATGCA+AGG + chr6.2:4489435-4489454 None:intergenic 30.0%
!!! GATTCCGAATTTTAGGTTTT+AGG - chr6.2:4489623-4489642 MS.gene54739:intron 30.0%
AACCATCATACAAGGAAATC+AGG - chr6.2:4490234-4490253 MS.gene54739:intron 35.0%
ACAAAATTGTACCTTGGTCA+TGG - chr6.2:4490711-4490730 MS.gene54739:intron 35.0%
AGCAAATTAAGCTTGACGTT+AGG - chr6.2:4489108-4489127 MS.gene54739:CDS 35.0%
AGGAAATGCAATGCAATACA+TGG + chr6.2:4488938-4488957 None:intergenic 35.0%
AGGCCCTAAAACCTAAAATT+CGG + chr6.2:4489630-4489649 None:intergenic 35.0%
CAGTTAAATTCAAACTGGCA+TGG - chr6.2:4490683-4490702 MS.gene54739:intron 35.0%
CTTAATTCCAAAAGATCTCC+AGG - chr6.2:4489713-4489732 MS.gene54739:intron 35.0%
GGAGTTGTTTATTATGGATC+CGG + chr6.2:4490303-4490322 None:intergenic 35.0%
GGATTTAGACTTGACAGATT+CGG - chr6.2:4490778-4490797 MS.gene54739:intron 35.0%
GTAACCACAAAATTGTACCT+TGG - chr6.2:4490705-4490724 MS.gene54739:intron 35.0%
GTTATAATCACGATGAGTCA+AGG - chr6.2:4490333-4490352 MS.gene54739:intron 35.0%
TACAAGGAAATCAGGAGAAA+AGG - chr6.2:4490242-4490261 MS.gene54739:intron 35.0%
TCTAAATCCTCGTAACAGAT+AGG + chr6.2:4490767-4490786 None:intergenic 35.0%
TCTCTTAAGTCAATCCAAAC+AGG + chr6.2:4489650-4489669 None:intergenic 35.0%
! ACTGTTTGCGTTTAAAGCAT+CGG + chr6.2:4490456-4490475 None:intergenic 35.0%
! ATGACCAAGGTACAATTTTG+TGG + chr6.2:4490712-4490731 None:intergenic 35.0%
! TAACAGATAGGACATTGACT+AGG + chr6.2:4490755-4490774 None:intergenic 35.0%
! TCATATAGTTTTCTGCATCC+CGG + chr6.2:4490881-4490900 None:intergenic 35.0%
!! CACGTGTTGATTTCGATTAA+AGG + chr6.2:4489463-4489482 None:intergenic 35.0%
!! TAGTTTAGTTAGTCTTGGGA+GGG - chr6.2:4489545-4489564 MS.gene54739:intron 35.0%
!! TGTTGTAGTAAAGCCTTGTT+TGG + chr6.2:4489873-4489892 None:intergenic 35.0%
!! TTAGTTTAGTTAGTCTTGGG+AGG - chr6.2:4489544-4489563 MS.gene54739:intron 35.0%
!!! ATGTCATGGTTTTGGTGTTT+TGG - chr6.2:4489040-4489059 MS.gene54739:CDS 35.0%
!!! GAGTCAAGGAATGGTTTTAA+CGG - chr6.2:4490347-4490366 MS.gene54739:intron 35.0%
!!! GTTTTTTCAGACAAATGGCT+CGG - chr6.2:4489076-4489095 MS.gene54739:CDS 35.0%
AAACAGTAACGAGCATTCCA+TGG - chr6.2:4490469-4490488 MS.gene54739:CDS 40.0%
AATCACGATGAGTCAAGGAA+TGG - chr6.2:4490338-4490357 MS.gene54739:intron 40.0%
AATCAGGAGAAAAGGAAGAG+AGG - chr6.2:4490250-4490269 MS.gene54739:intron 40.0%
ACTTAACGGTGAAAGAGTTC+AGG - chr6.2:4490561-4490580 MS.gene54739:intron 40.0%
AGATGAAATCCACACGAACA+AGG + chr6.2:4490812-4490831 None:intergenic 40.0%
ATTATTGTTCGCCATGACCA+AGG + chr6.2:4490725-4490744 None:intergenic 40.0%
CTCCTGATTTCCTTGTATGA+TGG + chr6.2:4490239-4490258 None:intergenic 40.0%
GAATTTCTTCCTTGTTCGTG+TGG - chr6.2:4490800-4490819 MS.gene54739:intron 40.0%
GGAGAATCAACCATCATACA+AGG - chr6.2:4490226-4490245 MS.gene54739:intron 40.0%
TAGCATCTGTTTGATCAGCA+TGG + chr6.2:4489930-4489949 None:intergenic 40.0%
TCAATGTCCTATCTGTTACG+AGG - chr6.2:4490757-4490776 MS.gene54739:intron 40.0%
TTCCTCACTTAGACTTCCTA+AGG + chr6.2:4490402-4490421 None:intergenic 40.0%
TTCCTTAGGAAGTCTAAGTG+AGG - chr6.2:4490397-4490416 MS.gene54739:intron 40.0%
TTGTCACAAAAAGATCGTCG+AGG - chr6.2:4490841-4490860 MS.gene54739:intron 40.0%
TTTCTCCTCCAAGAAGAAGA+GGG - chr6.2:4488632-4488651 MS.gene54739:CDS 40.0%
! GGACTTAACGATGGACTTAA+CGG - chr6.2:4490547-4490566 MS.gene54739:intron 40.0%
!! GCTATTTTCATAAGCTGTCC+TGG + chr6.2:4489734-4489753 None:intergenic 40.0%
!! TATAGGAGACTCTGAAACCA+TGG + chr6.2:4490489-4490508 None:intergenic 40.0%
!! TGTTGATTCAGAAGCAGCTA+AGG - chr6.2:4490526-4490545 MS.gene54739:intron 40.0%
!!! GGTTTTGGTGTTTTGGCTTA+TGG - chr6.2:4489047-4489066 MS.gene54739:CDS 40.0%
!!! TTTAGGTTTTAGGGCCTGTT+TGG - chr6.2:4489633-4489652 MS.gene54739:intron 40.0%
AAAAAGATCGTCGAGGCAGA+TGG - chr6.2:4490848-4490867 MS.gene54739:intron 45.0%
AGCAGCTAAGGACTTAACGA+TGG - chr6.2:4490538-4490557 MS.gene54739:intron 45.0%
AGGAGAAAAGGAAGAGAGGA+TGG - chr6.2:4490254-4490273 MS.gene54739:intron 45.0%
AGGGTTTACCCTCTTCTTCT+TGG + chr6.2:4488643-4488662 None:intergenic 45.0%
AGTCATGCCGTATGAGTACA+GGG - chr6.2:4490957-4490976 MS.gene54739:CDS 45.0%
CTCGCAATGTGCTTAGATGA+TGG + chr6.2:4490203-4490222 None:intergenic 45.0%
CTCTTCTTCTTGGAGGAGAA+AGG + chr6.2:4488633-4488652 None:intergenic 45.0%
CTTTCTCCTCCAAGAAGAAG+AGG - chr6.2:4488631-4488650 MS.gene54739:CDS 45.0%
GGAAGTCTAAGTGAGGAAGA+CGG - chr6.2:4490404-4490423 MS.gene54739:intron 45.0%
GTTTACCCTCTTCTTCTTGG+AGG + chr6.2:4488640-4488659 None:intergenic 45.0%
TCTTCTTGGAGGAGAAAGGA+GGG + chr6.2:4488629-4488648 None:intergenic 45.0%
TGCATCCAAGCGAATGATTC+CGG - chr6.2:4490281-4490300 MS.gene54739:intron 45.0%
TTCTTCTTGGAGGAGAAAGG+AGG + chr6.2:4488630-4488649 None:intergenic 45.0%
! AAGACGGATGCCTTGATGAT+TGG - chr6.2:4490420-4490439 MS.gene54739:intron 45.0%
! AAGCTGTCCTGGAGATCTTT+TGG + chr6.2:4489723-4489742 None:intergenic 45.0%
! AGACGGATGCCTTGATGATT+GGG - chr6.2:4490421-4490440 MS.gene54739:intron 45.0%
! AGCACGAGTACGTTTTCCTT+AGG - chr6.2:4490383-4490402 MS.gene54739:intron 45.0%
!! CGATGTTGACTCAACTGGAA+GGG - chr6.2:4490166-4490185 MS.gene54739:intron 45.0%
!! TGCGTTTAAAGCATCGGCAA+CGG + chr6.2:4490450-4490469 None:intergenic 45.0%
!! AAAATGAATAAAAATTAAAA+AGG + chr6.2:4488669-4488688 None:intergenic 5.0%
ATCTAAGCACATTGCGAGCG+AGG - chr6.2:4490205-4490224 MS.gene54739:intron 50.0%
CACATATGAGCTGTCGAGCA+TGG - chr6.2:4490600-4490619 MS.gene54739:intron 50.0%
CAGTCATGCCGTATGAGTAC+AGG - chr6.2:4490956-4490975 MS.gene54739:CDS 50.0%
CGCGAACAATGGCTTTCTAG+AGG - chr6.2:4489131-4489150 MS.gene54739:CDS 50.0%
GGAGAAAGGAGGGTCAGAAA+TGG + chr6.2:4488619-4488638 None:intergenic 50.0%
TGGATCCGGAATCATTCGCT+TGG + chr6.2:4490289-4490308 None:intergenic 50.0%
TTGACGTTAGGCGCGAACAA+TGG - chr6.2:4489120-4489139 MS.gene54739:CDS 50.0%
!! AGTTAGTCTTGGGAGGGACA+TGG - chr6.2:4489551-4489570 MS.gene54739:intron 50.0%
!! GAGAGTTATGCGGCTTTGAG+AGG + chr6.2:4490664-4490683 None:intergenic 50.0%
!! GCGATGTTGACTCAACTGGA+AGG - chr6.2:4490165-4490184 MS.gene54739:intron 50.0%
!! GGATGCGATGTTGACTCAAC+TGG - chr6.2:4490161-4490180 MS.gene54739:intron 50.0%
!! GTTATGCGGCTTTGAGAGGT+CGG + chr6.2:4490660-4490679 None:intergenic 50.0%
!! TTATGCGGCTTTGAGAGGTC+GGG + chr6.2:4490659-4490678 None:intergenic 50.0%
CAACGGCCTCCCAATCATCA+AGG + chr6.2:4490433-4490452 None:intergenic 55.0%
GGAGGGTCAGAAATGGAACC+AGG + chr6.2:4488612-4488631 None:intergenic 55.0%
! CGGATGCCTTGATGATTGGG+AGG - chr6.2:4490424-4490443 MS.gene54739:intron 55.0%
!! CCTCGGACGAAGAGTGTCAT+CGG + chr6.2:4490630-4490649 None:intergenic 55.0%
!! CTCGGACGAAGAGTGTCATC+GGG + chr6.2:4490629-4490648 None:intergenic 55.0%
CCGATGACACTCTTCGTCCG+AGG - chr6.2:4490627-4490646 MS.gene54739:intron 60.0%
CGAGGCAGATGGACGATGTC+CGG - chr6.2:4490859-4490878 MS.gene54739:intron 60.0%
GAGAGGTCGGGCAAACACCT+CGG + chr6.2:4490647-4490666 None:intergenic 60.0%
GAGGCAGATGGACGATGTCC+GGG - chr6.2:4490860-4490879 MS.gene54739:intron 60.0%


Chromosome Type Strat End Strand Name
chr6.2 gene 4488603 4490985 4488603 ID=MS.gene54739
chr6.2 mRNA 4488603 4490985 4488603 ID=MS.gene54739.t1;Parent=MS.gene54739
chr6.2 exon 4490935 4490985 4490935 ID=MS.gene54739.t1.exon1;Parent=MS.gene54739.t1
chr6.2 CDS 4490935 4490985 4490935 ID=cds.MS.gene54739.t1;Parent=MS.gene54739.t1
chr6.2 exon 4490436 4490502 4490436 ID=MS.gene54739.t1.exon2;Parent=MS.gene54739.t1
chr6.2 CDS 4490436 4490502 4490436 ID=cds.MS.gene54739.t1;Parent=MS.gene54739.t1
chr6.2 exon 4488603 4489435 4488603 ID=MS.gene54739.t1.exon3;Parent=MS.gene54739.t1
chr6.2 CDS 4488603 4489435 4488603 ID=cds.MS.gene54739.t1;Parent=MS.gene54739.t1
Gene Sequence

>MS.gene54739

ATGGCTCCTGGTTCCATTTCTGACCCTCCTTTCTCCTCCAAGAAGAAGAGGGTAAACCCTTCTCCTTTTTAATTTTTATTCATTTTTGTTTTATTGTATTTTTTTTTTCAAGCCCTTGAGCTATAAAAATTTGATTTTTTTTTTGTTTGTGATTTTTCAATATTGAGAGATAAAAATTTGATTTTTTGTAAAACCCATTTTCCATTTCATTGCATTTTGTTTTGTTGAGTTCAAAGGGTAGCAAATTCTATATTTCTTTCTATGTTTTGTTGTTCTTGGAAAATCTTCATTTTTTATGTCATGGTTTTATCATAAAGGTTCATTCTCATTTTCCATGTATTGCATTGCATTTCCTTTTATGACATTCATTATGTTTTTCCTCTAAGGAAGCAATTTTTCTTTTTTGCTTTGTTTTTGATGTTTTGGAAATTTTCATTATGTCATGGTTTTGGTGTTTTGGCTTATGGTATTGTGTTTTTTCAGACAAATGGCTCGGCGAAACTGAAGCAAATTAAGCTTGACGTTAGGCGCGAACAATGGCTTTCTAGAGGTGACTTTATGATCATCATTTTCATATGGAATTTTTTTGACATATTGATTATGTTTTTCATTGTTCAATCAACCAATGATAAGATTTTTCTTTTTGCCTTCATAATCTTGCCTTCATAATCATGAATCTTGAATGTCATTCTGTTATTTATGTAAGGGTTTAGAGGATTTAGAGATTTTTGAAGATTTCTAGAGATGTTTGTTGATTCTCTGATATTGACATGCATTAGTCCTTTGATCCATTCGCTATTATATTTTTTAAATATTACTTTGTTTGATTCCTTGCATTATCTACCAAAATGATTAGGCCTTTAATCGAAATCAACACGTGTGAAATGTTTTACATGTGAACTTTCAAATTGTAACATTCAAACTAATATGACATTCAAAGCTTAGTTTAGTTAGTCTTGGGAGGGACATGGCTTCTCAACTGATCATGTTTGAAGCATCATGTGACTGTCTTTTATCATTGATTCCGAATTTTAGGTTTTAGGGCCTGTTTGGATTGACTTAAGAGAGTTTATGGAAACAGCTTATGACATATTCATAAGCTGTTTTTATCTTAATTCCAAAAGATCTCCAGGACAGCTTATGAAAATAGCTTATAGCTTATATAAAATTAGTTTGACTTTATTTTATCTTTTATTATAGAAATAGCTTATAGATAAGCACTACATGATCAGCATTTATGCTATAAGAGCTTAATTATACTGTTTATCCAAACAAGGCTTTACTACAACAACAGTCAAATCTTTTTCTCACTAGATGGGATTAGCCATGCTGATCAAACAGATGCTATAGATAAATATTAAACAGAATTCTAAGTGAATTAAATATATTGTTTCTCAATGGAATGCAAGCAATAGTTGTAATATCATTTTATTTTAAAAAAATTCAAAGATGGTAAAAACAATAGTTATGAATTGCTTAAAAATCCTTTTGAAGTCGGTAACTTTTTCATTACTTAAATGTTAACTGTTGATCATACTTTTGTTTTTCAGTCAAAAAGGGATGCGATGTTGACTCAACTGGAAGGGTTGATAGTGATCCATCATCTAAGCACATTGCGAGCGAGGAGAATCAACCATCATACAAGGAAATCAGGAGAAAAGGAAGAGAGGATGGTACATGCATCCAAGCGAATGATTCCGGATCCATAATAAACAACTCCATTCAAAGTAGTTATAATCACGATGAGTCAAGGAATGGTTTTAACGGAAGTAGAAGCAGCAGCACGAGTACGTTTTCCTTAGGAAGTCTAAGTGAGGAAGACGGATGCCTTGATGATTGGGAGGCCGTTGCCGATGCTTTAAACGCAAACAGTAACGAGCATTCCATGGTTTCAGAGTCTCCTATAGAGCATACGATTAATTATGTTGATTCAGAAGCAGCTAAGGACTTAACGATGGACTTAACGGTGAAAGAGTTCAGGTCAGCAGTTTCTGAATCACATATGAGCTGTCGAGCATGGAAGCCCGATGACACTCTTCGTCCGAGGTGTTTGCCCGACCTCTCAAAGCCGCATAACTCTCAGTTAAATTCAAACTGGCATGGTAACCACAAAATTGTACCTTGGTCATGGCGAACAATAATTTCTCAACCTAGTCAATGTCCTATCTGTTACGAGGATTTAGACTTGACAGATTCGGAATTTCTTCCTTGTTCGTGTGGATTTCATCTCTGCCTTTTTTGTCACAAAAAGATCGTCGAGGCAGATGGACGATGTCCGGGATGCAGAAAACTATATGATCATGTAGATGGAAATGTTGAATTCAATACAGAAACAAATACTTTTTATATGACTCAGTCATGCCGTATGAGTACAGGGTGCTAG
Protein sequence

>MS.gene54739.t1

MAPGSISDPPFSSKKKRTNGSAKLKQIKLDVRREQWLSRVKKGCDVDSTGRVDSDPSSKHIASEENQPSYKEIRRKGREDGTCIQANDSGSIINNSIQSSYNHDESRNGFNGSRSSSTSTFSLGSLSEEDGCLDDWEAVADALNANSNEHSMVSESPIEHTINYVDSEAAKDLTMDLTVKEFRSAVSESHMSCRAWKPDDTLRPRCLPDLSKPHNSQLNSNWHGNHKIVPWSWRTIISQPSQCPICYEDLDLTDSEFLPCSCGFHLCLFCHKKIVEADGRCPGCRKLYDHVDGNVEFNTETNTFYMTQSCRMSTGC