Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54807.t1 | XP_003591885.2 | 99.6 | 469 | 2 | 0 | 1 | 469 | 1 | 469 | 8.40E-264 | 919.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54807.t1 | Q9ZUA0 | 69.8 | 440 | 133 | 0 | 27 | 466 | 22 | 461 | 6.3e-183 | 641.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54807.t1 | G7I373 | 99.6 | 469 | 2 | 0 | 1 | 469 | 1 | 469 | 6.1e-264 | 919.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene039903 | MS.gene54807 | PPI |
| MS.gene003786 | MS.gene54807 | PPI |
| MS.gene001011 | MS.gene54807 | PPI |
| MS.gene50024 | MS.gene54807 | PPI |
| MS.gene057990 | MS.gene54807 | PPI |
| MS.gene50925 | MS.gene54807 | PPI |
| MS.gene50921 | MS.gene54807 | PPI |
| MS.gene05210 | MS.gene54807 | PPI |
| MS.gene45709 | MS.gene54807 | PPI |
| MS.gene057988 | MS.gene54807 | PPI |
| MS.gene056676 | MS.gene54807 | PPI |
| MS.gene37721 | MS.gene54807 | PPI |
| MS.gene50052 | MS.gene54807 | PPI |
| MS.gene039901 | MS.gene54807 | PPI |
| MS.gene57836 | MS.gene54807 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54807.t1 | MTR_1g094870 | 99.574 | 469 | 2 | 0 | 1 | 469 | 1 | 469 | 0.0 | 949 |
| MS.gene54807.t1 | MTR_3g115980 | 55.455 | 440 | 193 | 2 | 32 | 469 | 29 | 467 | 1.82e-169 | 491 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54807.t1 | AT2G01720 | 71.267 | 442 | 127 | 0 | 27 | 468 | 22 | 463 | 0.0 | 672 |
| MS.gene54807.t1 | AT1G76400 | 54.042 | 433 | 197 | 2 | 38 | 469 | 33 | 464 | 1.37e-160 | 466 |
| MS.gene54807.t1 | AT1G76400 | 54.042 | 433 | 197 | 2 | 38 | 469 | 33 | 464 | 5.95e-160 | 466 |
Find 117 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACCTCGGTATCCTCTATT+TGG | 0.193369 | 1.2:-73339685 | MS.gene54807:CDS |
| TCCACCTTTCTTCTTGGTTA+TGG | 0.252341 | 1.2:-73339652 | MS.gene54807:CDS |
| GGGAAGGACTGTGGTGGTTT+TGG | 0.252829 | 1.2:-73337520 | MS.gene54807:CDS |
| GCTGGTACAGAGCTGAAATA+TGG | 0.269457 | 1.2:-73341165 | MS.gene54807:CDS |
| TGGCTCAGCAAGCATGAATA+TGG | 0.274282 | 1.2:+73336337 | None:intergenic |
| TCTGAATTCTCACATAATTA+AGG | 0.297263 | 1.2:-73342488 | MS.gene54807:CDS |
| GTATACTACCGGGATGAAAT+AGG | 0.303864 | 1.2:-73340268 | MS.gene54807:CDS |
| TGGAAATCCACCTTTCTTCT+TGG | 0.308439 | 1.2:-73339658 | MS.gene54807:CDS |
| TTTCTACCGGGAAAGGTTCC+AGG | 0.313712 | 1.2:+73341331 | None:intergenic |
| TATATCGCTCTGTGATTTGT+AGG | 0.315214 | 1.2:+73341022 | None:intergenic |
| ACATTCCCTGGAACCTTTCC+CGG | 0.319281 | 1.2:-73341337 | MS.gene54807:CDS |
| TGAGCATAACATCCCTTTCC+AGG | 0.345316 | 1.2:-73337478 | MS.gene54807:intron |
| AGATACCTCAACTTTACTTA+TGG | 0.346751 | 1.2:-73339586 | MS.gene54807:CDS |
| TGAAATTGAAATATCTCATT+GGG | 0.353686 | 1.2:-73341049 | MS.gene54807:CDS |
| AATCACGGCTGCAGGATCTT+TGG | 0.355677 | 1.2:+73339412 | None:intergenic |
| TGCCATCTGGAGACTCAAAA+AGG | 0.375711 | 1.2:+73339611 | None:intergenic |
| AGTGGAGTTAACAAGGTTAC+AGG | 0.382914 | 1.2:+73341405 | None:intergenic |
| TTAGATCCATTTGGTCCATC+AGG | 0.392359 | 1.2:+73341435 | None:intergenic |
| AGGAGATGAGTTAACGAGAA+TGG | 0.395486 | 1.2:+73342814 | None:intergenic |
| TTACATATTAACACATTCCC+TGG | 0.402653 | 1.2:-73341349 | MS.gene54807:CDS |
| AGTCTTCTTCTTCCTCTTCC+CGG | 0.405895 | 1.2:+73341491 | None:intergenic |
| TACGGTGTCTGCTCGTCATA+TGG | 0.416487 | 1.2:+73341141 | None:intergenic |
| TTCTGTGACATATTTACATA+TGG | 0.417654 | 1.2:-73336276 | MS.gene54807:CDS |
| GTGAAATTGAAATATCTCAT+TGG | 0.418999 | 1.2:-73341050 | MS.gene54807:CDS |
| CTCTGTACCAGCACGTTTAG+TGG | 0.422155 | 1.2:+73341176 | None:intergenic |
| TTGAGGTATCTTCTGCCATC+TGG | 0.422948 | 1.2:+73339598 | None:intergenic |
| TTTGAGTACTGCTAGATGTT+CGG | 0.425836 | 1.2:+73341527 | None:intergenic |
| CTCAAAGCTGCCGCAACTAC+CGG | 0.434593 | 1.2:-73341510 | MS.gene54807:CDS |
| TTAGTTAGTTACTCTGCGTT+CGG | 0.453470 | 1.2:+73342766 | None:intergenic |
| AATACAGATGCTAGCATCAA+TGG | 0.458197 | 1.2:+73336317 | None:intergenic |
| AGATGTTGATCTACTTGAAA+AGG | 0.459409 | 1.2:+73339389 | None:intergenic |
| GGCTCAGCAAGCATGAATAT+GGG | 0.460787 | 1.2:+73336338 | None:intergenic |
| GCAGGATCTTTGGATCCCTC+TGG | 0.464700 | 1.2:+73339422 | None:intergenic |
| GTTGAATATCAAACTAGATC+AGG | 0.471474 | 1.2:-73340352 | MS.gene54807:CDS |
| AGCTCCTCAACAACGGCAAA+GGG | 0.482451 | 1.2:+73341078 | None:intergenic |
| TGAAAAGGAATCACGGCTGC+AGG | 0.482684 | 1.2:+73339404 | None:intergenic |
| GTTTGTTGCTTGACATGATA+AGG | 0.487559 | 1.2:+73341252 | None:intergenic |
| TGACTTATTTCTACCGGGAA+AGG | 0.493316 | 1.2:+73341324 | None:intergenic |
| TGGTTGCCCTCTTGCTCAGA+CGG | 0.493408 | 1.2:-73339566 | MS.gene54807:CDS |
| CATTAAGACTCCAACCGCTC+GGG | 0.494237 | 1.2:-73341223 | MS.gene54807:CDS |
| TCTGATTGACTTATTTCTAC+CGG | 0.494339 | 1.2:+73341318 | None:intergenic |
| TTCGGCTTCAGCAGGTGAAA+AGG | 0.496327 | 1.2:+73341545 | None:intergenic |
| AGAAGTGAAGGAAGAGAAAG+AGG | 0.499599 | 1.2:+73342837 | None:intergenic |
| CTGATTGACTTATTTCTACC+GGG | 0.500739 | 1.2:+73341319 | None:intergenic |
| TAAGGTCTACTTGACATTGA+AGG | 0.501289 | 1.2:-73342470 | MS.gene54807:intron |
| TTCTCAAAATGAACAAGTAC+AGG | 0.504398 | 1.2:+73341108 | None:intergenic |
| GGTATCCTCTATTTGGAGGC+TGG | 0.513462 | 1.2:-73339678 | MS.gene54807:CDS |
| AATGTCAAGGTATGAATACT+TGG | 0.515014 | 1.2:+73337545 | None:intergenic |
| CAATCCCTTTGCCGTTGTTG+AGG | 0.515385 | 1.2:-73341082 | MS.gene54807:CDS |
| TATTCATACCTTGACATTGT+GGG | 0.517201 | 1.2:-73337540 | MS.gene54807:CDS |
| GGGTTCACTCAGTATACTAC+CGG | 0.517261 | 1.2:-73340279 | MS.gene54807:CDS |
| TAACGAGAATGGAGAAGTGA+AGG | 0.518224 | 1.2:+73342825 | None:intergenic |
| CTGGTACAGAGCTGAAATAT+GGG | 0.519780 | 1.2:-73341164 | MS.gene54807:CDS |
| TCATTAAGACTCCAACCGCT+CGG | 0.520181 | 1.2:-73341224 | MS.gene54807:CDS |
| GCTAGATGTTCGGCTTCAGC+AGG | 0.521174 | 1.2:+73341537 | None:intergenic |
| GATTTCCAGCCTCCAAATAG+AGG | 0.522219 | 1.2:+73339673 | None:intergenic |
| GCAAGATGTACTTCTGAAGC+AGG | 0.523168 | 1.2:+73341567 | None:intergenic |
| GAGATACCTGATGGACCAAA+TGG | 0.524355 | 1.2:-73341441 | MS.gene54807:CDS |
| CGATATAAGCTGGTTCATGC+TGG | 0.524532 | 1.2:-73341006 | MS.gene54807:CDS |
| CCTCGGTATCCTCTATTTGG+AGG | 0.524884 | 1.2:-73339682 | MS.gene54807:CDS |
| TAGTATACTGAGTGAACCCT+AGG | 0.526754 | 1.2:+73340283 | None:intergenic |
| TAGCTCCTCAACAACGGCAA+AGG | 0.528305 | 1.2:+73341077 | None:intergenic |
| AGTTGTTCATGACTTGCGTA+TGG | 0.542230 | 1.2:+73336247 | None:intergenic |
| CTGCCGCAACTACCGGGAAG+AGG | 0.542930 | 1.2:-73341503 | MS.gene54807:CDS |
| GCTTGGTTGATACCTGGAAA+GGG | 0.547719 | 1.2:+73337466 | None:intergenic |
| TCAAAGCTGCCGCAACTACC+GGG | 0.549883 | 1.2:-73341509 | MS.gene54807:CDS |
| TCTGTACCAGCACGTTTAGT+GGG | 0.557172 | 1.2:+73341177 | None:intergenic |
| TTGTTAACTCCACTGAGTAA+AGG | 0.559974 | 1.2:-73341396 | MS.gene54807:CDS |
| GGCTTGGTTGATACCTGGAA+AGG | 0.560827 | 1.2:+73337465 | None:intergenic |
| TTCTACCGGGAAAGGTTCCA+GGG | 0.561417 | 1.2:+73341332 | None:intergenic |
| AATGATTCTACCCGAGCGGT+TGG | 0.563337 | 1.2:+73341213 | None:intergenic |
| AATCACAGAGCGATATAAGC+TGG | 0.566072 | 1.2:-73341016 | MS.gene54807:CDS |
| TTCACCATCAATGGTCGCCA+TGG | 0.567692 | 1.2:-73342885 | None:intergenic |
| TCAACCACCGTCTGAGCAAG+AGG | 0.568747 | 1.2:+73339559 | None:intergenic |
| TTTACTCAGTGGAGTTAACA+AGG | 0.569462 | 1.2:+73341398 | None:intergenic |
| AGTACAGGAGAATATGAATA+CGG | 0.571755 | 1.2:+73341123 | None:intergenic |
| CAAACTAGATCAGGTGCTAG+TGG | 0.578545 | 1.2:-73340343 | MS.gene54807:CDS |
| TGGAAAGGGATGTTATGCTC+AGG | 0.578762 | 1.2:+73337480 | None:intergenic |
| ATTTAGGTTGTGCTTCCAGA+GGG | 0.579918 | 1.2:-73339437 | MS.gene54807:intron |
| TAGTCCATAACCAAGAAGAA+AGG | 0.583269 | 1.2:+73339648 | None:intergenic |
| ACAATAATATTGCACTATCA+CGG | 0.583872 | 1.2:+73341277 | None:intergenic |
| ATCTACTTGAAAAGGAATCA+CGG | 0.585368 | 1.2:+73339397 | None:intergenic |
| TCAGCAGATTTAACATCAAG+CGG | 0.586004 | 1.2:+73341462 | None:intergenic |
| AAATCTGCTGAGATACCTGA+TGG | 0.587325 | 1.2:-73341450 | MS.gene54807:CDS |
| TTGCCCTCTTGCTCAGACGG+TGG | 0.588234 | 1.2:-73339563 | MS.gene54807:CDS |
| TCCATAACCAAGAAGAAAGG+TGG | 0.588608 | 1.2:+73339651 | None:intergenic |
| TCAAGTAGATCAACATCTAG+AGG | 0.590429 | 1.2:-73339384 | MS.gene54807:intron |
| TGTGAGCAGAGGTTGTTCCA+TGG | 0.592128 | 1.2:+73342868 | None:intergenic |
| CTTCCTCTTCCCGGTAGTTG+CGG | 0.595971 | 1.2:+73341500 | None:intergenic |
| GGCAACCATAAGTAAAGTTG+AGG | 0.597837 | 1.2:+73339581 | None:intergenic |
| CATTGTGGGAAGGACTGTGG+TGG | 0.603609 | 1.2:-73337526 | MS.gene54807:CDS |
| GTAGAGCCCACTAAACGTGC+TGG | 0.605387 | 1.2:-73341183 | MS.gene54807:CDS |
| GTCGTTTCACCTTTACTCAG+TGG | 0.607230 | 1.2:+73341387 | None:intergenic |
| ATGATCTGAAGGTGTTGAGA+AGG | 0.613509 | 1.2:+73342794 | None:intergenic |
| GTTCGGCATTGATGATCTGA+AGG | 0.617044 | 1.2:+73342783 | None:intergenic |
| GTATTCATACCTTGACATTG+TGG | 0.618837 | 1.2:-73337541 | MS.gene54807:CDS |
| AAATATTGCCTATTTCATCC+CGG | 0.619277 | 1.2:+73340260 | None:intergenic |
| AGTATACTGAGTGAACCCTA+GGG | 0.619575 | 1.2:+73340284 | None:intergenic |
| AATTTAGGTTGTGCTTCCAG+AGG | 0.621224 | 1.2:-73339438 | MS.gene54807:intron |
| AAGTCAATCAGAGTCGCAGT+TGG | 0.629557 | 1.2:-73341307 | MS.gene54807:CDS |
| AGGAGTGCGATTGTGAGCAG+AGG | 0.631692 | 1.2:+73342857 | None:intergenic |
| CATGCTGGTGCTAGACACAA+AGG | 0.634853 | 1.2:-73340991 | MS.gene54807:CDS |
| CAGTCCTTCCCACAATGTCA+AGG | 0.636145 | 1.2:+73337532 | None:intergenic |
| AGGTGAAACGACAACACTTG+AGG | 0.641720 | 1.2:-73341376 | MS.gene54807:CDS |
| ACGTTCTAGCTCCTCAACAA+CGG | 0.642984 | 1.2:+73341071 | None:intergenic |
| GTGGAGTTAACAAGGTTACA+GGG | 0.646599 | 1.2:+73341406 | None:intergenic |
| GCTCAGCAAGCATGAATATG+GGG | 0.655765 | 1.2:+73336339 | None:intergenic |
| CAACCACCGTCTGAGCAAGA+GGG | 0.657316 | 1.2:+73339560 | None:intergenic |
| CATACCTTGACATTGTGGGA+AGG | 0.658291 | 1.2:-73337536 | MS.gene54807:CDS |
| CAGAACTTGAATTTGAACCT+CGG | 0.660616 | 1.2:-73339699 | MS.gene54807:CDS |
| GAAATTGAAATATCTCATTG+GGG | 0.680457 | 1.2:-73341048 | MS.gene54807:CDS |
| GGTTCACTCAGTATACTACC+GGG | 0.708588 | 1.2:-73340278 | MS.gene54807:CDS |
| CCTCCAAATAGAGGATACCG+AGG | 0.713300 | 1.2:+73339682 | None:intergenic |
| TGTGAATGATTCTACCCGAG+CGG | 0.716578 | 1.2:+73341209 | None:intergenic |
| TGACATTGTGGGAAGGACTG+TGG | 0.720812 | 1.2:-73337529 | MS.gene54807:CDS |
| GTATACTGAGTGAACCCTAG+GGG | 0.721984 | 1.2:+73340285 | None:intergenic |
| TATACTGAGTGAACCCTAGG+GGG | 0.733345 | 1.2:+73340286 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 73336253 | 73342897 | 73336253 | ID=MS.gene54807 |
| chr1.2 | mRNA | 73336253 | 73342897 | 73336253 | ID=MS.gene54807.t1;Parent=MS.gene54807 |
| chr1.2 | exon | 73342778 | 73342897 | 73342778 | ID=MS.gene54807.t1.exon1;Parent=MS.gene54807.t1 |
| chr1.2 | CDS | 73342778 | 73342897 | 73342778 | ID=cds.MS.gene54807.t1;Parent=MS.gene54807.t1 |
| chr1.2 | exon | 73342471 | 73342515 | 73342471 | ID=MS.gene54807.t1.exon2;Parent=MS.gene54807.t1 |
| chr1.2 | CDS | 73342471 | 73342515 | 73342471 | ID=cds.MS.gene54807.t1;Parent=MS.gene54807.t1 |
| chr1.2 | exon | 73340979 | 73341610 | 73340979 | ID=MS.gene54807.t1.exon3;Parent=MS.gene54807.t1 |
| chr1.2 | CDS | 73340979 | 73341610 | 73340979 | ID=cds.MS.gene54807.t1;Parent=MS.gene54807.t1 |
| chr1.2 | exon | 73340228 | 73340375 | 73340228 | ID=MS.gene54807.t1.exon4;Parent=MS.gene54807.t1 |
| chr1.2 | CDS | 73340228 | 73340375 | 73340228 | ID=cds.MS.gene54807.t1;Parent=MS.gene54807.t1 |
| chr1.2 | exon | 73339543 | 73339722 | 73339543 | ID=MS.gene54807.t1.exon5;Parent=MS.gene54807.t1 |
| chr1.2 | CDS | 73339543 | 73339722 | 73339543 | ID=cds.MS.gene54807.t1;Parent=MS.gene54807.t1 |
| chr1.2 | exon | 73339385 | 73339453 | 73339385 | ID=MS.gene54807.t1.exon6;Parent=MS.gene54807.t1 |
| chr1.2 | CDS | 73339385 | 73339453 | 73339385 | ID=cds.MS.gene54807.t1;Parent=MS.gene54807.t1 |
| chr1.2 | exon | 73337479 | 73337568 | 73337479 | ID=MS.gene54807.t1.exon7;Parent=MS.gene54807.t1 |
| chr1.2 | CDS | 73337479 | 73337568 | 73337479 | ID=cds.MS.gene54807.t1;Parent=MS.gene54807.t1 |
| chr1.2 | exon | 73336253 | 73336378 | 73336253 | ID=MS.gene54807.t1.exon8;Parent=MS.gene54807.t1 |
| chr1.2 | CDS | 73336253 | 73336378 | 73336253 | ID=cds.MS.gene54807.t1;Parent=MS.gene54807.t1 |
| Gene Sequence |
| Protein sequence |
