Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54946.t1 | XP_003624498.1 | 97.9 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 3.20E-73 | 284.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54946.t1 | Q96500 | 49.7 | 143 | 65 | 4 | 1 | 141 | 1 | 138 | 3.1e-24 | 112.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54946.t1 | B7FGR7 | 97.9 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 2.3e-73 | 284.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050076 | MS.gene54946 | 0.831292 | 1.81E-55 | -1.69E-46 |
| MS.gene050404 | MS.gene54946 | 0.883271 | 5.17E-71 | -1.69E-46 |
| MS.gene050406 | MS.gene54946 | 0.864532 | 1.14E-64 | -1.69E-46 |
| MS.gene050529 | MS.gene54946 | 0.801162 | 1.03E-48 | -1.69E-46 |
| MS.gene050783 | MS.gene54946 | 0.803917 | 2.77E-49 | -1.69E-46 |
| MS.gene05101 | MS.gene54946 | 0.802839 | 4.64E-49 | -1.69E-46 |
| MS.gene051171 | MS.gene54946 | 0.848539 | 5.75E-60 | -1.69E-46 |
| MS.gene051240 | MS.gene54946 | 0.816541 | 5.24E-52 | -1.69E-46 |
| MS.gene051242 | MS.gene54946 | 0.844016 | 9.81E-59 | -1.69E-46 |
| MS.gene051244 | MS.gene54946 | 0.818776 | 1.64E-52 | -1.69E-46 |
| MS.gene051471 | MS.gene54946 | 0.814775 | 1.30E-51 | -1.69E-46 |
| MS.gene052290 | MS.gene54946 | 0.830072 | 3.61E-55 | -1.69E-46 |
| MS.gene053907 | MS.gene54946 | 0.83625 | 1.04E-56 | -1.69E-46 |
| MS.gene053971 | MS.gene54946 | 0.870731 | 1.17E-66 | -1.69E-46 |
| MS.gene055254 | MS.gene54946 | 0.830017 | 3.72E-55 | -1.69E-46 |
| MS.gene056612 | MS.gene54946 | 0.838594 | 2.62E-57 | -1.69E-46 |
| MS.gene056633 | MS.gene54946 | 0.82501 | 5.92E-54 | -1.69E-46 |
| MS.gene056664 | MS.gene54946 | 0.841773 | 3.88E-58 | -1.69E-46 |
| MS.gene057295 | MS.gene54946 | 0.800187 | 1.62E-48 | -1.69E-46 |
| MS.gene058233 | MS.gene54946 | 0.837144 | 6.18E-57 | -1.69E-46 |
| MS.gene058276 | MS.gene54946 | 0.827634 | 1.40E-54 | -1.69E-46 |
| MS.gene058293 | MS.gene54946 | 0.803062 | 4.17E-49 | -1.69E-46 |
| MS.gene058746 | MS.gene54946 | 0.844698 | 6.44E-59 | -1.69E-46 |
| MS.gene059858 | MS.gene54946 | 0.800061 | 1.72E-48 | -1.69E-46 |
| MS.gene059894 | MS.gene54946 | 0.846526 | 2.06E-59 | -1.69E-46 |
| MS.gene060407 | MS.gene54946 | 0.803961 | 2.72E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene54946 | 0.811885 | 5.60E-51 | -1.69E-46 |
| MS.gene061112 | MS.gene54946 | 0.816538 | 5.25E-52 | -1.69E-46 |
| MS.gene061265 | MS.gene54946 | 0.802435 | 5.62E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54946.t1 | MTR_7g084000 | 97.917 | 144 | 3 | 0 | 1 | 144 | 1 | 144 | 2.06e-103 | 291 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54946.t1 | AT3G26740 | 48.611 | 144 | 65 | 4 | 1 | 141 | 1 | 138 | 6.75e-33 | 113 |
Find 36 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACTCTTTGGTGTTGTTAA+AGG | 0.238854 | 7.2:-23491235 | None:intergenic |
| GGTGTATCATTCCTTGCTTC+AGG | 0.283991 | 7.2:-23491960 | None:intergenic |
| TCAGGCTGCAGCTTGACTTC+AGG | 0.292025 | 7.2:-23491942 | None:intergenic |
| AGAACGTGAGGCAATCAATT+TGG | 0.330098 | 7.2:-23491290 | None:intergenic |
| TTCCCTGAAGCTGCAGTCTT+TGG | 0.331736 | 7.2:-23491981 | None:intergenic |
| ACACCAAAGACTGCAGCTTC+AGG | 0.337411 | 7.2:+23491978 | MS.gene54946:CDS |
| GGTTGGAGGGGATGCTTGTT+TGG | 0.357304 | 7.2:+23491908 | MS.gene54946:CDS |
| ATAGCGAAATCTTACGTCTT+AGG | 0.366730 | 7.2:-23492035 | None:intergenic |
| GCAAGATGGGAGACACTCTT+TGG | 0.409104 | 7.2:-23491247 | None:intergenic |
| CTGCGAGCCTGATTATCAAA+AGG | 0.412863 | 7.2:+23492195 | MS.gene54946:CDS |
| ACCGTTTCACATGCCTCAGC+TGG | 0.450987 | 7.2:-23491888 | None:intergenic |
| GCAGAGGCTTGTGATGATCT+TGG | 0.462484 | 7.2:+23492163 | MS.gene54946:CDS |
| CACCAAAGACTGCAGCTTCA+GGG | 0.489453 | 7.2:+23491979 | MS.gene54946:CDS |
| TGCGAGCCTGATTATCAAAA+GGG | 0.500804 | 7.2:+23492196 | MS.gene54946:CDS |
| AACTCAAATTAGTAGCAAGA+TGG | 0.505177 | 7.2:-23491261 | None:intergenic |
| GATCATCACAAGCCTCTGCT+TGG | 0.508891 | 7.2:-23492159 | None:intergenic |
| ACTCAAATTAGTAGCAAGAT+GGG | 0.522705 | 7.2:-23491260 | None:intergenic |
| TGTTGAAGGATCAGAGTTGT+CGG | 0.523519 | 7.2:-23491347 | None:intergenic |
| TGTTAAAGGCACAAAGGTAG+TGG | 0.526517 | 7.2:-23491221 | None:intergenic |
| CAGCTTCAGGGAACATTGAG+AGG | 0.539207 | 7.2:+23491991 | MS.gene54946:CDS |
| CGTGAGGCAATCAATTTGGT+AGG | 0.539328 | 7.2:-23491286 | None:intergenic |
| TCCAGCTGAGGCATGTGAAA+CGG | 0.549406 | 7.2:+23491887 | MS.gene54946:CDS |
| GAATTGTAATCAACTGTTGA+AGG | 0.559497 | 7.2:-23491361 | None:intergenic |
| AGGCATGTGAAACGGTTGGA+GGG | 0.565003 | 7.2:+23491895 | MS.gene54946:CDS |
| GCTGAGGCATGTGAAACGGT+TGG | 0.574341 | 7.2:+23491891 | MS.gene54946:CDS |
| TGTCAGAGTGTTTCCAGCTG+AGG | 0.580476 | 7.2:+23491875 | MS.gene54946:intron |
| CACAAAGGTAGTGGTTGCAA+AGG | 0.582454 | 7.2:-23491212 | None:intergenic |
| GAGGCATGTGAAACGGTTGG+AGG | 0.591598 | 7.2:+23491894 | MS.gene54946:CDS |
| TCAAGCTGCAGCCTGAAGCA+AGG | 0.602199 | 7.2:+23491949 | MS.gene54946:CDS |
| GAGGCTTGTGATGATCTTGG+AGG | 0.607345 | 7.2:+23492166 | MS.gene54946:CDS |
| GGCATGTGAAACGGTTGGAG+GGG | 0.610004 | 7.2:+23491896 | MS.gene54946:CDS |
| GTGAGGCAATCAATTTGGTA+GGG | 0.637953 | 7.2:-23491285 | None:intergenic |
| TGGTGTTGTTAAAGGCACAA+AGG | 0.647032 | 7.2:-23491227 | None:intergenic |
| AGCTTCAGGGAACATTGAGA+GGG | 0.650830 | 7.2:+23491992 | MS.gene54946:CDS |
| GCGAGGTGAAGAAGAACGTG+AGG | 0.676435 | 7.2:-23491302 | None:intergenic |
| ACTTTGATTGCAGTGTAGCG+AGG | 0.698323 | 7.2:-23491319 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATATTATATTTATAACCTT+AGG | + | chr7.2:23491479-23491498 | MS.gene54946:intron | 10.0% |
| !! | AACTAAACTAAAATGAATTA+AGG | - | chr7.2:23491417-23491436 | None:intergenic | 15.0% |
| !!! | AATTCATTTTAGTTTAGTTT+AGG | + | chr7.2:23491418-23491437 | MS.gene54946:intron | 15.0% |
| !!! | TAGTATATTATTAGTTTCTA+TGG | + | chr7.2:23491549-23491568 | MS.gene54946:intron | 15.0% |
| !! | AATAACACAAACAAATGTTA+TGG | - | chr7.2:23491849-23491868 | None:intergenic | 20.0% |
| !!! | ATCTCTATAAGTTATTCTTT+TGG | + | chr7.2:23491768-23491787 | MS.gene54946:intron | 20.0% |
| !!! | TATTATTAGTTTCTATGGTA+TGG | + | chr7.2:23491554-23491573 | MS.gene54946:intron | 20.0% |
| ! | ATCAGAATTGTGATGTTAAA+TGG | + | chr7.2:23492113-23492132 | MS.gene54946:intron | 25.0% |
| !! | TTTGTTTCAGGACTAAATTT+TGG | + | chr7.2:23491802-23491821 | MS.gene54946:intron | 25.0% |
| !!! | AGTTTAGTTTAGGTTTTCTT+AGG | + | chr7.2:23491428-23491447 | MS.gene54946:intron | 25.0% |
| !!! | GACTTTTAGAATTTTGTTTC+AGG | + | chr7.2:23491790-23491809 | MS.gene54946:intron | 25.0% |
| AACTCAAATTAGTAGCAAGA+TGG | - | chr7.2:23491264-23491283 | None:intergenic | 30.0% | |
| ACTCAAATTAGTAGCAAGAT+GGG | - | chr7.2:23491263-23491282 | None:intergenic | 30.0% | |
| AGATCGTGCTCATTATTTAT+AGG | + | chr7.2:23491508-23491527 | MS.gene54946:intron | 30.0% | |
| ATTAAGGTGATAGAGAATGA+AGG | - | chr7.2:23491401-23491420 | None:intergenic | 30.0% | |
| GAATTGTAATCAACTGTTGA+AGG | - | chr7.2:23491364-23491383 | None:intergenic | 30.0% | |
| GATTACCATTTGTCACAAAT+AGG | - | chr7.2:23491626-23491645 | None:intergenic | 30.0% | |
| !!! | AATGGATGATGTGTTTTTTC+AGG | + | chr7.2:23492131-23492150 | MS.gene54946:intron | 30.0% |
| !!! | ATGGATGATGTGTTTTTTCA+GGG | + | chr7.2:23492132-23492151 | MS.gene54946:intron | 30.0% |
| ATAGCGAAATCTTACGTCTT+AGG | - | chr7.2:23492038-23492057 | None:intergenic | 35.0% | |
| GCATACCTATTTGTGACAAA+TGG | + | chr7.2:23491618-23491637 | MS.gene54946:intron | 35.0% | |
| !! | AATTTTGGAGAGTCACAACA+TGG | + | chr7.2:23491817-23491836 | MS.gene54946:intron | 35.0% |
| !! | ACACTCTTTGGTGTTGTTAA+AGG | - | chr7.2:23491238-23491257 | None:intergenic | 35.0% |
| !! | TAGACACCCTTTTGATAATC+AGG | - | chr7.2:23492205-23492224 | None:intergenic | 35.0% |
| AGAACGTGAGGCAATCAATT+TGG | - | chr7.2:23491293-23491312 | None:intergenic | 40.0% | |
| GTGAGGCAATCAATTTGGTA+GGG | - | chr7.2:23491288-23491307 | None:intergenic | 40.0% | |
| TGCGAGCCTGATTATCAAAA+GGG | + | chr7.2:23492196-23492215 | MS.gene54946:CDS | 40.0% | |
| TGTTGAAGGATCAGAGTTGT+CGG | - | chr7.2:23491350-23491369 | None:intergenic | 40.0% | |
| ! | TGTTAAAGGCACAAAGGTAG+TGG | - | chr7.2:23491224-23491243 | None:intergenic | 40.0% |
| !! | TGGTGTTGTTAAAGGCACAA+AGG | - | chr7.2:23491230-23491249 | None:intergenic | 40.0% |
| ACTTTGATTGCAGTGTAGCG+AGG | - | chr7.2:23491322-23491341 | None:intergenic | 45.0% | |
| AGCACGATCTGTACTTCCTA+AGG | - | chr7.2:23491498-23491517 | None:intergenic | 45.0% | |
| AGCTTCAGGGAACATTGAGA+GGG | + | chr7.2:23491992-23492011 | MS.gene54946:CDS | 45.0% | |
| CGTGAGGCAATCAATTTGGT+AGG | - | chr7.2:23491289-23491308 | None:intergenic | 45.0% | |
| CTGCGAGCCTGATTATCAAA+AGG | + | chr7.2:23492195-23492214 | MS.gene54946:CDS | 45.0% | |
| GGTGTATCATTCCTTGCTTC+AGG | - | chr7.2:23491963-23491982 | None:intergenic | 45.0% | |
| ! | CACAAAGGTAGTGGTTGCAA+AGG | - | chr7.2:23491215-23491234 | None:intergenic | 45.0% |
| ! | TTTCAGGGTTTTCCAAGCAG+AGG | + | chr7.2:23492147-23492166 | MS.gene54946:intron | 45.0% |
| ACACCAAAGACTGCAGCTTC+AGG | + | chr7.2:23491978-23491997 | MS.gene54946:CDS | 50.0% | |
| AGGCATGTGAAACGGTTGGA+GGG | + | chr7.2:23491895-23491914 | MS.gene54946:CDS | 50.0% | |
| CACCAAAGACTGCAGCTTCA+GGG | + | chr7.2:23491979-23491998 | MS.gene54946:CDS | 50.0% | |
| CAGCTTCAGGGAACATTGAG+AGG | + | chr7.2:23491991-23492010 | MS.gene54946:CDS | 50.0% | |
| GAGGCTTGTGATGATCTTGG+AGG | + | chr7.2:23492166-23492185 | MS.gene54946:CDS | 50.0% | |
| GATCATCACAAGCCTCTGCT+TGG | - | chr7.2:23492162-23492181 | None:intergenic | 50.0% | |
| GCAAGATGGGAGACACTCTT+TGG | - | chr7.2:23491250-23491269 | None:intergenic | 50.0% | |
| GCAGAGGCTTGTGATGATCT+TGG | + | chr7.2:23492163-23492182 | MS.gene54946:CDS | 50.0% | |
| TCCAGCTGAGGCATGTGAAA+CGG | + | chr7.2:23491887-23491906 | MS.gene54946:CDS | 50.0% | |
| TTCCCTGAAGCTGCAGTCTT+TGG | - | chr7.2:23491984-23492003 | None:intergenic | 50.0% | |
| ! | TGTCAGAGTGTTTCCAGCTG+AGG | + | chr7.2:23491875-23491894 | MS.gene54946:intron | 50.0% |
| ACCGTTTCACATGCCTCAGC+TGG | - | chr7.2:23491891-23491910 | None:intergenic | 55.0% | |
| GAGGCATGTGAAACGGTTGG+AGG | + | chr7.2:23491894-23491913 | MS.gene54946:CDS | 55.0% | |
| GCGAGGTGAAGAAGAACGTG+AGG | - | chr7.2:23491305-23491324 | None:intergenic | 55.0% | |
| GCTGAGGCATGTGAAACGGT+TGG | + | chr7.2:23491891-23491910 | MS.gene54946:CDS | 55.0% | |
| GGCATGTGAAACGGTTGGAG+GGG | + | chr7.2:23491896-23491915 | MS.gene54946:CDS | 55.0% | |
| GGTTGGAGGGGATGCTTGTT+TGG | + | chr7.2:23491908-23491927 | MS.gene54946:CDS | 55.0% | |
| TCAAGCTGCAGCCTGAAGCA+AGG | + | chr7.2:23491949-23491968 | MS.gene54946:CDS | 55.0% | |
| TCAGGCTGCAGCTTGACTTC+AGG | - | chr7.2:23491945-23491964 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 23491196 | 23492228 | 23491196 | ID=MS.gene54946 |
| chr7.2 | mRNA | 23491196 | 23492228 | 23491196 | ID=MS.gene54946.t1;Parent=MS.gene54946 |
| chr7.2 | exon | 23491196 | 23491392 | 23491196 | ID=MS.gene54946.t1.exon1;Parent=MS.gene54946.t1 |
| chr7.2 | CDS | 23491196 | 23491392 | 23491196 | ID=cds.MS.gene54946.t1;Parent=MS.gene54946.t1 |
| chr7.2 | exon | 23491881 | 23492042 | 23491881 | ID=MS.gene54946.t1.exon2;Parent=MS.gene54946.t1 |
| chr7.2 | CDS | 23491881 | 23492042 | 23491881 | ID=cds.MS.gene54946.t1;Parent=MS.gene54946.t1 |
| chr7.2 | exon | 23492153 | 23492228 | 23492153 | ID=MS.gene54946.t1.exon3;Parent=MS.gene54946.t1 |
| chr7.2 | CDS | 23492153 | 23492228 | 23492153 | ID=cds.MS.gene54946.t1;Parent=MS.gene54946.t1 |
| Gene Sequence |
| Protein sequence |
