AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55208


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55208.t1 MTR_5g040340 91.368 475 24 4 1 468 1 465 0.0 864
MS.gene55208.t1 MTR_8g055860 46.667 510 167 24 17 468 29 491 2.87e-102 314
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55208.t1 AT2G02950 36.585 410 174 20 55 443 76 420 8.18e-47 168
MS.gene55208.t1 AT1G14280 33.967 421 178 22 52 439 63 416 6.91e-43 157

Find 71 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAAGAAGATGGAGAAAT+TGG 0.158218 5.2:-30314713 MS.gene55208:CDS
GAATTAAACGATGTTCTTAT+TGG 0.158853 5.2:+30313762 None:intergenic
TCACTTGGTGCATAGAAATT+TGG 0.171246 5.2:+30313882 None:intergenic
GCCTCAGATGACAAGGTTTA+AGG 0.196735 5.2:-30313592 MS.gene55208:CDS
TTTCAAGTACTTGAGTTTAT+TGG 0.250362 5.2:+30314622 None:intergenic
TGCACTTTCTATTCTCTTGT+TGG 0.253513 5.2:+30314597 None:intergenic
TTGCATCTTTCATCTAATAT+TGG 0.263596 5.2:+30314146 None:intergenic
CTTGAGTTTATTGGCATCTA+AGG 0.270047 5.2:+30314631 None:intergenic
TTCCTTTCTGTTGCTGATAA+AGG 0.278838 5.2:+30314757 None:intergenic
TCCTTTCTGTTGCTGATAAA+GGG 0.296457 5.2:+30314758 None:intergenic
AGGTTTAAGGTGGAAAGTTT+TGG 0.354957 5.2:-30313579 MS.gene55208:CDS
AAGAGTCTTCTTGCTTCTCT+TGG 0.359154 5.2:-30314440 MS.gene55208:CDS
AGGAAAACATTGGAAGTGTT+TGG 0.359214 5.2:-30314173 MS.gene55208:CDS
AGAAAGTGCAAAGTTCAATA+TGG 0.368278 5.2:-30314584 MS.gene55208:CDS
TCAACATCACATGTTGCTTC+TGG 0.368292 5.2:-30313915 MS.gene55208:CDS
CATTCAATGCTTGCTTCACT+TGG 0.371898 5.2:+30313867 None:intergenic
AGAAATGAACAACATGGTTA+TGG 0.378020 5.2:-30313552 MS.gene55208:CDS
GTTATGGAAAACCTCCTCTT+AGG 0.383306 5.2:-30313536 MS.gene55208:CDS
GCGGCGTCCGGGAAAATTGT+TGG 0.404010 5.2:-30313700 MS.gene55208:CDS
AGAGATGCCGAGGCGGCGTC+CGG 0.408283 5.2:-30313712 MS.gene55208:CDS
CGGCGTCCGGGAAAATTGTT+GGG 0.423128 5.2:-30313699 MS.gene55208:CDS
TATGAACTTGGTTATGAACT+TGG 0.432001 5.2:+30314465 None:intergenic
CAGAAAGCTGTTGATGTTGC+TGG 0.436890 5.2:-30313666 MS.gene55208:CDS
CTTGATTCTGAGCCAACACT+TGG 0.447581 5.2:+30314557 None:intergenic
CTTCTTCTTTCTTTACTCCA+AGG 0.457657 5.2:+30314726 None:intergenic
AGTCAATAAGCTTTGATATG+AGG 0.461128 5.2:-30314124 MS.gene55208:CDS
TTCAACAAAAGTGCAGTTCA+AGG 0.462298 5.2:-30314353 MS.gene55208:CDS
AAGGTTCAGTAGTAACTATA+AGG 0.462753 5.2:-30313788 MS.gene55208:CDS
TTTGAGATTGAAAGCTTCAC+TGG 0.464240 5.2:-30313969 MS.gene55208:CDS
GAAGACTCTTTCTATGAACT+TGG 0.474532 5.2:+30314453 None:intergenic
GAGATGCCGAGGCGGCGTCC+GGG 0.478167 5.2:-30313711 MS.gene55208:CDS
TTCGAAGCAGCTTCCCAAGA+TGG 0.484347 5.2:+30314092 None:intergenic
GCATCTAAGGTAGCAAGTCT+TGG 0.492321 5.2:+30314644 None:intergenic
CTTGAACAACAAGGAAGTAA+AGG 0.492569 5.2:-30314804 MS.gene55208:CDS
TTCTGTTGCTGATAAAGGGG+TGG 0.493694 5.2:+30314762 None:intergenic
TTCCGCTGCAGCACGAGTCT+CGG 0.494266 5.2:+30314259 None:intergenic
GTTTCAGATCATGAAGATCA+AGG 0.495758 5.2:-30313807 MS.gene55208:CDS
CAAGTGAAGCAAGCATTGAA+TGG 0.508457 5.2:-30313866 MS.gene55208:CDS
GGGTGGATTGAATCAGCAAA+AGG 0.523505 5.2:+30314779 None:intergenic
ACATCGTTTAATTCATCGAA+TGG 0.528036 5.2:-30313753 MS.gene55208:CDS
TTCTTTACTCCAAGGTGAAG+AGG 0.529025 5.2:+30314734 None:intergenic
CCTTTCTGTTGCTGATAAAG+GGG 0.530407 5.2:+30314759 None:intergenic
GGAGTAAAGAAAGAAGAAGA+TGG 0.538350 5.2:-30314722 MS.gene55208:CDS
ACCTTAAACCTTGTCATCTG+AGG 0.538940 5.2:+30313591 None:intergenic
AACCGAGACTCGTGCTGCAG+CGG 0.552008 5.2:-30314261 MS.gene55208:CDS
TTCAACAAGTAGAGAATCCA+AGG 0.552480 5.2:-30314193 MS.gene55208:CDS
CCCCTTTATCAGCAACAGAA+AGG 0.557673 5.2:-30314759 MS.gene55208:CDS
AGAGAATCCAAGGAAAACAT+TGG 0.562983 5.2:-30314183 MS.gene55208:CDS
GGTGAATTTGATCTCCTAAG+AGG 0.568136 5.2:+30313522 None:intergenic
GTTATTCTGAGAGGTGAAAG+TGG 0.569101 5.2:+30314862 None:intergenic
GTTATTGTTGTTATTCTGAG+AGG 0.577463 5.2:+30314853 None:intergenic
CAGGTTGTAAAAGTGCACTT+TGG 0.581251 5.2:+30314525 None:intergenic
CAATATGGAACTCCAAGTGT+TGG 0.581850 5.2:-30314569 MS.gene55208:CDS
GAATTTGATCTCCTAAGAGG+AGG 0.592977 5.2:+30313525 None:intergenic
CTCTTCATACTTGAACAACA+AGG 0.600465 5.2:-30314813 MS.gene55208:CDS
AGAAAGGAACCTCTTCACCT+TGG 0.604048 5.2:-30314743 MS.gene55208:CDS
AAATGTTGCCTCAGATGACA+AGG 0.605171 5.2:-30313599 MS.gene55208:CDS
TCAGATGACAAGGTTTAAGG+TGG 0.630245 5.2:-30313589 MS.gene55208:CDS
AAATCAACAAAGAGATGCCG+AGG 0.636605 5.2:-30313722 MS.gene55208:CDS
GGTGCAAGAAATGAACAACA+TGG 0.655080 5.2:-30313558 MS.gene55208:CDS
TCAACAAAGAGATGCCGAGG+CGG 0.670252 5.2:-30313719 MS.gene55208:CDS
AACAACAATAACCTCCGTGG+TGG 0.687252 5.2:-30314842 MS.gene55208:CDS
GTTGATGTAAGTGATCATGC+AGG 0.688347 5.2:-30314386 MS.gene55208:CDS
AGGAGATCAAATTCACCACA+TGG 0.692006 5.2:-30313516 MS.gene55208:CDS
ACAACAACAAATGAGAAACA+TGG 0.697642 5.2:-30313633 MS.gene55208:CDS
AGCAAGCATTGAATGGAGTG+TGG 0.726329 5.2:-30313859 MS.gene55208:CDS
ATGAAGAGAAAGAACCACCA+CGG 0.734637 5.2:+30314828 None:intergenic
TACAAAAGTTGTGCACCATG+TGG 0.736641 5.2:+30313501 None:intergenic
AATAACAACAATAACCTCCG+TGG 0.749739 5.2:-30314845 MS.gene55208:CDS
AGTGGTTGCTGAGATAACCA+TGG 0.758373 5.2:+30314880 None:intergenic
AAGAGAAAGAACCACCACGG+AGG 0.793634 5.2:+30314831 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAATTAAACGATGTTCTTAT+TGG + chr5.2:30314613-30314632 None:intergenic 25.0%
! TGTTCTTGATAAAAATGAGT+TGG + chr5.2:30314437-30314456 None:intergenic 25.0%
! TTGCATCTTTCATCTAATAT+TGG + chr5.2:30314229-30314248 None:intergenic 25.0%
!! ATGTTTGAAGAATTTTTCTG+AGG + chr5.2:30314053-30314072 None:intergenic 25.0%
!! TTTCAAGTACTTGAGTTTAT+TGG + chr5.2:30313753-30313772 None:intergenic 25.0%
AAAGAAGAAGATGGAGAAAT+TGG - chr5.2:30313659-30313678 MS.gene55208:CDS 30.0%
AAGGTTCAGTAGTAACTATA+AGG - chr5.2:30314584-30314603 MS.gene55208:CDS 30.0%
ACAACAACAAATGAGAAACA+TGG - chr5.2:30314739-30314758 MS.gene55208:CDS 30.0%
ACATCGTTTAATTCATCGAA+TGG - chr5.2:30314619-30314638 MS.gene55208:CDS 30.0%
AGAAATGAACAACATGGTTA+TGG - chr5.2:30314820-30314839 MS.gene55208:CDS 30.0%
GATGCTGAAAAATACTTCAA+TGG - chr5.2:30313689-30313708 MS.gene55208:CDS 30.0%
TATGAACTTGGTTATGAACT+TGG + chr5.2:30313910-30313929 None:intergenic 30.0%
! AGAAAGTGCAAAGTTCAATA+TGG - chr5.2:30313788-30313807 MS.gene55208:CDS 30.0%
! AGTCAATAAGCTTTGATATG+AGG - chr5.2:30314248-30314267 MS.gene55208:CDS 30.0%
! GATAATGATGATGATGATGT+TGG - chr5.2:30314358-30314377 MS.gene55208:CDS 30.0%
! GTTATTGTTGTTATTCTGAG+AGG + chr5.2:30313522-30313541 None:intergenic 30.0%
!! GAAGTAGAATTTTTCAAAGC+AGG + chr5.2:30313869-30313888 None:intergenic 30.0%
AATAACAACAATAACCTCCG+TGG - chr5.2:30313527-30313546 MS.gene55208:CDS 35.0%
AGAGAATCCAAGGAAAACAT+TGG - chr5.2:30314189-30314208 MS.gene55208:CDS 35.0%
CTCTTCATACTTGAACAACA+AGG - chr5.2:30313559-30313578 MS.gene55208:CDS 35.0%
CTTCTTCTTTCTTTACTCCA+AGG + chr5.2:30313649-30313668 None:intergenic 35.0%
CTTGAACAACAAGGAAGTAA+AGG - chr5.2:30313568-30313587 MS.gene55208:CDS 35.0%
CTTGAGTTTATTGGCATCTA+AGG + chr5.2:30313744-30313763 None:intergenic 35.0%
GAAGACTCTTTCTATGAACT+TGG + chr5.2:30313922-30313941 None:intergenic 35.0%
GAGGCTAAAAATACCATCTT+GGG - chr5.2:30314267-30314286 MS.gene55208:CDS 35.0%
GGAGTAAAGAAAGAAGAAGA+TGG - chr5.2:30313650-30313669 MS.gene55208:CDS 35.0%
GTTTCAGATCATGAAGATCA+AGG - chr5.2:30314565-30314584 MS.gene55208:CDS 35.0%
TCCTTTCTGTTGCTGATAAA+GGG + chr5.2:30313617-30313636 None:intergenic 35.0%
TGAGGCTAAAAATACCATCT+TGG - chr5.2:30314266-30314285 MS.gene55208:CDS 35.0%
TGCACTTTCTATTCTCTTGT+TGG + chr5.2:30313778-30313797 None:intergenic 35.0%
TTCAACAAGTAGAGAATCCA+AGG - chr5.2:30314179-30314198 MS.gene55208:CDS 35.0%
TTCCTTTCTGTTGCTGATAA+AGG + chr5.2:30313618-30313637 None:intergenic 35.0%
! AACACTTCCAATGTTTTCCT+TGG + chr5.2:30314199-30314218 None:intergenic 35.0%
! TTACAACCCAACAATTTTCC+CGG + chr5.2:30314682-30314701 None:intergenic 35.0%
! TTCAACAAAAGTGCAGTTCA+AGG - chr5.2:30314019-30314038 MS.gene55208:CDS 35.0%
! TTTGAGATTGAAAGCTTCAC+TGG - chr5.2:30314403-30314422 MS.gene55208:CDS 35.0%
!! AGGAAAACATTGGAAGTGTT+TGG - chr5.2:30314199-30314218 MS.gene55208:CDS 35.0%
!! AGGTTTAAGGTGGAAAGTTT+TGG - chr5.2:30314793-30314812 MS.gene55208:CDS 35.0%
!! TCACTTGGTGCATAGAAATT+TGG + chr5.2:30314493-30314512 None:intergenic 35.0%
AAATCAACAAAGAGATGCCG+AGG - chr5.2:30314650-30314669 MS.gene55208:CDS 40.0%
AAATGTTGCCTCAGATGACA+AGG - chr5.2:30314773-30314792 MS.gene55208:CDS 40.0%
AAGAGTCTTCTTGCTTCTCT+TGG - chr5.2:30313932-30313951 MS.gene55208:CDS 40.0%
ACCTTAAACCTTGTCATCTG+AGG + chr5.2:30314784-30314803 None:intergenic 40.0%
AGGAGATCAAATTCACCACA+TGG - chr5.2:30314856-30314875 MS.gene55208:CDS 40.0%
ATGAAGAGAAAGAACCACCA+CGG + chr5.2:30313547-30313566 None:intergenic 40.0%
CAATATGGAACTCCAAGTGT+TGG - chr5.2:30313803-30313822 MS.gene55208:CDS 40.0%
CATTCAATGCTTGCTTCACT+TGG + chr5.2:30314508-30314527 None:intergenic 40.0%
CCTTTCTGTTGCTGATAAAG+GGG + chr5.2:30313616-30313635 None:intergenic 40.0%
GAATTTGATCTCCTAAGAGG+AGG + chr5.2:30314850-30314869 None:intergenic 40.0%
GGTGAATTTGATCTCCTAAG+AGG + chr5.2:30314853-30314872 None:intergenic 40.0%
GGTGCAAGAAATGAACAACA+TGG - chr5.2:30314814-30314833 MS.gene55208:CDS 40.0%
GTTATGGAAAACCTCCTCTT+AGG - chr5.2:30314836-30314855 MS.gene55208:CDS 40.0%
GTTGATGTAAGTGATCATGC+AGG - chr5.2:30313986-30314005 MS.gene55208:CDS 40.0%
TACAAAAGTTGTGCACCATG+TGG + chr5.2:30314874-30314893 None:intergenic 40.0%
TCAGATGACAAGGTTTAAGG+TGG - chr5.2:30314783-30314802 MS.gene55208:CDS 40.0%
TTCTTTACTCCAAGGTGAAG+AGG + chr5.2:30313641-30313660 None:intergenic 40.0%
! CAGGTTGTAAAAGTGCACTT+TGG + chr5.2:30313850-30313869 None:intergenic 40.0%
! GTTATTCTGAGAGGTGAAAG+TGG + chr5.2:30313513-30313532 None:intergenic 40.0%
! TCAACATCACATGTTGCTTC+TGG - chr5.2:30314457-30314476 MS.gene55208:CDS 40.0%
!! CAAGTGAAGCAAGCATTGAA+TGG - chr5.2:30314506-30314525 MS.gene55208:CDS 40.0%
!! GTCATCTGAGGCAACATTTT+TGG + chr5.2:30314772-30314791 None:intergenic 40.0%
AACAACAATAACCTCCGTGG+TGG - chr5.2:30313530-30313549 MS.gene55208:CDS 45.0%
AGAAAGGAACCTCTTCACCT+TGG - chr5.2:30313629-30313648 MS.gene55208:CDS 45.0%
CAGAAAGCTGTTGATGTTGC+TGG - chr5.2:30314706-30314725 MS.gene55208:CDS 45.0%
CCCCTTTATCAGCAACAGAA+AGG - chr5.2:30313613-30313632 MS.gene55208:CDS 45.0%
CTTGATTCTGAGCCAACACT+TGG + chr5.2:30313818-30313837 None:intergenic 45.0%
GCCTCAGATGACAAGGTTTA+AGG - chr5.2:30314780-30314799 MS.gene55208:CDS 45.0%
TTCTGTTGCTGATAAAGGGG+TGG + chr5.2:30313613-30313632 None:intergenic 45.0%
! GCATCTAAGGTAGCAAGTCT+TGG + chr5.2:30313731-30313750 None:intergenic 45.0%
!! AGCAAGCATTGAATGGAGTG+TGG - chr5.2:30314513-30314532 MS.gene55208:CDS 45.0%
!! GGGTGGATTGAATCAGCAAA+AGG + chr5.2:30313596-30313615 None:intergenic 45.0%
AAGAGAAAGAACCACCACGG+AGG + chr5.2:30313544-30313563 None:intergenic 50.0%
TCAACAAAGAGATGCCGAGG+CGG - chr5.2:30314653-30314672 MS.gene55208:CDS 50.0%
TTCGAAGCAGCTTCCCAAGA+TGG + chr5.2:30314283-30314302 None:intergenic 50.0%
CGGCGTCCGGGAAAATTGTT+GGG - chr5.2:30314673-30314692 MS.gene55208:CDS 55.0%
AACCGAGACTCGTGCTGCAG+CGG - chr5.2:30314111-30314130 MS.gene55208:CDS 60.0%
GCGGCGTCCGGGAAAATTGT+TGG - chr5.2:30314672-30314691 MS.gene55208:CDS 60.0%
TTCCGCTGCAGCACGAGTCT+CGG + chr5.2:30314116-30314135 None:intergenic 60.0%
! AATTTTCCCGGACGCCGCCT+CGG + chr5.2:30314670-30314689 None:intergenic 60.0%
AGAGATGCCGAGGCGGCGTC+CGG - chr5.2:30314660-30314679 MS.gene55208:CDS 70.0%
GAGATGCCGAGGCGGCGTCC+GGG - chr5.2:30314661-30314680 MS.gene55208:CDS 75.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 30313494 30314900 30313494 ID=MS.gene55208
chr5.2 mRNA 30313494 30314900 30313494 ID=MS.gene55208.t1;Parent=MS.gene55208
chr5.2 exon 30313494 30314900 30313494 ID=MS.gene55208.t1.exon1;Parent=MS.gene55208.t1
chr5.2 CDS 30313494 30314900 30313494 ID=cds.MS.gene55208.t1;Parent=MS.gene55208.t1
Gene Sequence

>MS.gene55208

ATGGTTATCTCAGCAACCACTTTCACCTCTCAGAATAACAACAATAACCTCCGTGGTGGTTCTTTCTCTTCATACTTGAACAACAAGGAAGTAAAGGATCCTTTTGCTGATTCAATCCACCCCTTTATCAGCAACAGAAAGGAACCTCTTCACCTTGGAGTAAAGAAAGAAGAAGATGGAGAAATTGGAGTATTTGATGCTGAAAAATACTTCAATGGAGTTGAAATAGAAACTCCAAGACTTGCTACCTTAGATGCCAATAAACTCAAGTACTTGAAAGACCAACAAGAGAATAGAAAGTGCAAAGTTCAATATGGAACTCCAAGTGTTGGCTCAGAATCAAGCTTGAATAGCCAAAGTGCACTTTTACAACCTGCTTTGAAAAATTCTACTTCAAGTAATAGTAGAAAAAACCAAGTTCATAACCAAGTTCATAGAAAGAGTCTTCTTGCTTCTCTTGGTTGCAAATGCTCTTGTTATGATAAGAATTCTGTTGATGTAAGTGATCATGCAGGTGAAATCAGCTTCAACAAAAGTGCAGTTCAAGGAAAAACAACCTCAGAAAAATTCTTCAAACATGATCAAGATGCTTCTGATCATTCAATTAAGAATAAGAAAACCGAGACTCGTGCTGCAGCGGAACTCTTGATGAACAAAGAAAAGATTTCTACTTACAAAATGCAACTTCAACAAGTAGAGAATCCAAGGAAAACATTGGAAGTGTTTGGTTCACCAATATTAGATGAAAGATGCAAGTCAATAAGCTTTGATATGAGGCTAAAAATACCATCTTGGGAAGCTGCTTCGAAACTTGAAGAAGAAGAAGAAATTGATTTTTCTACAAATTGTGATGAAAACAACTATGATAATGATGATGATGATGTTGGAAGTGATGCAAGTTCAGATTTATTTGAGATTGAAAGCTTCACTGGTAATAAAACCAACTCATTTTTATCAAGAACATCAACATCACATGTTGCTTCTGGTTGCACTAGTCCAAATTTCTATGCACCAAGTGAAGCAAGCATTGAATGGAGTGTGGTAACTGCAAGTGCAGTTGATTATTCAGCTGTTTCAGATCATGAAGATCAAGGTTCAGTAGTAACTATAAGGAGTCCAATAAGAACATCGTTTAATTCATCGAATGGTAAACACAAAATCAACAAAGAGATGCCGAGGCGGCGTCCGGGAAAATTGTTGGGTTGTAATAGTCAGAAAGCTGTTGATGTTGCTGGTGATGCTTTCACAACAACAAATGAGAAACATGGTACAAATCCAAAAATGTTGCCTCAGATGACAAGGTTTAAGGTGGAAAGTTTTGGTGCAAGAAATGAACAACATGGTTATGGAAAACCTCCTCTTAGGAGATCAAATTCACCACATGGTGCACAACTTTTGTATATTTAG

Protein sequence

>MS.gene55208.t1

MVISATTFTSQNNNNNLRGGSFSSYLNNKEVKDPFADSIHPFISNRKEPLHLGVKKEEDGEIGVFDAEKYFNGVEIETPRLATLDANKLKYLKDQQENRKCKVQYGTPSVGSESSLNSQSALLQPALKNSTSSNSRKNQVHNQVHRKSLLASLGCKCSCYDKNSVDVSDHAGEISFNKSAVQGKTTSEKFFKHDQDASDHSIKNKKTETRAAAELLMNKEKISTYKMQLQQVENPRKTLEVFGSPILDERCKSISFDMRLKIPSWEAASKLEEEEEIDFSTNCDENNYDNDDDDVGSDASSDLFEIESFTGNKTNSFLSRTSTSHVASGCTSPNFYAPSEASIEWSVVTASAVDYSAVSDHEDQGSVVTIRSPIRTSFNSSNGKHKINKEMPRRRPGKLLGCNSQKAVDVAGDAFTTTNEKHGTNPKMLPQMTRFKVESFGARNEQHGYGKPPLRRSNSPHGAQLLYI