Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55755.t1 | XP_013447130.1 | 92.5 | 147 | 11 | 0 | 1 | 147 | 81 | 227 | 2.20E-69 | 271.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55755.t1 | A0A072TUP3 | 92.5 | 147 | 11 | 0 | 1 | 147 | 81 | 227 | 1.6e-69 | 271.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050961 | MS.gene55755 | 0.821887 | 3.18E-53 | -1.69E-46 |
| MS.gene051959 | MS.gene55755 | 0.851733 | 7.32E-61 | -1.69E-46 |
| MS.gene051960 | MS.gene55755 | 0.846507 | 2.08E-59 | -1.69E-46 |
| MS.gene052053 | MS.gene55755 | 0.836675 | 8.14E-57 | -1.69E-46 |
| MS.gene052559 | MS.gene55755 | 0.813741 | 2.20E-51 | -1.69E-46 |
| MS.gene053932 | MS.gene55755 | 0.816713 | 4.80E-52 | -1.69E-46 |
| MS.gene054892 | MS.gene55755 | 0.837698 | 4.46E-57 | -1.69E-46 |
| MS.gene054893 | MS.gene55755 | 0.84383 | 1.10E-58 | -1.69E-46 |
| MS.gene055150 | MS.gene55755 | 0.819004 | 1.46E-52 | -1.69E-46 |
| MS.gene055549 | MS.gene55755 | 0.806519 | 7.91E-50 | -1.69E-46 |
| MS.gene055550 | MS.gene55755 | 0.806946 | 6.43E-50 | -1.69E-46 |
| MS.gene055551 | MS.gene55755 | 0.812564 | 3.98E-51 | -1.69E-46 |
| MS.gene05624 | MS.gene55755 | 0.804058 | 2.59E-49 | -1.69E-46 |
| MS.gene057001 | MS.gene55755 | 0.802183 | 6.33E-49 | -1.69E-46 |
| MS.gene057093 | MS.gene55755 | 0.810937 | 8.99E-51 | -1.69E-46 |
| MS.gene057622 | MS.gene55755 | 0.807525 | 4.84E-50 | -1.69E-46 |
| MS.gene058592 | MS.gene55755 | 0.825378 | 4.84E-54 | -1.69E-46 |
| MS.gene05878 | MS.gene55755 | 0.837945 | 3.85E-57 | -1.69E-46 |
| MS.gene05952 | MS.gene55755 | 0.833935 | 4.01E-56 | -1.69E-46 |
| MS.gene06023 | MS.gene55755 | 0.994432 | 3.31E-207 | -1.69E-46 |
| MS.gene060413 | MS.gene55755 | 0.803906 | 2.79E-49 | -1.69E-46 |
| MS.gene060964 | MS.gene55755 | 0.817645 | 2.96E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55755.t1 | MTR_8g099035 | 92.517 | 147 | 11 | 0 | 1 | 147 | 81 | 227 | 2.85e-96 | 277 |
| MS.gene55755.t1 | MTR_8g028475 | 38.393 | 112 | 68 | 1 | 19 | 130 | 93 | 203 | 2.66e-21 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55755.t1 | AT2G46150 | 33.871 | 124 | 80 | 2 | 19 | 141 | 93 | 215 | 1.58e-19 | 81.3 |
| MS.gene55755.t1 | AT3G54200 | 31.868 | 91 | 62 | 0 | 25 | 115 | 114 | 204 | 8.75e-11 | 58.2 |
Find 31 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTGAAACTTCCACTTATA+AGG | 0.143507 | 4.3:+5174026 | None:intergenic |
| TATTGGATTCATTAATATTT+AGG | 0.155942 | 4.3:-5173618 | None:intergenic |
| ATACAAATTCATTCACTTTC+AGG | 0.174928 | 4.3:-5173952 | MS.gene55755:CDS |
| TTCTATAATGGAATTGATAT+TGG | 0.258144 | 4.3:-5173905 | MS.gene55755:CDS |
| AATATGTTCAAGAGGTCAAT+TGG | 0.340166 | 4.3:-5173707 | MS.gene55755:CDS |
| GTTGAAACTTCCACTTATAA+GGG | 0.363902 | 4.3:+5174027 | None:intergenic |
| ACACGTGGTGATAATTGTAT+TGG | 0.405537 | 4.3:-5173635 | MS.gene55755:CDS |
| AATTCATTCACTTTCAGGTA+TGG | 0.412565 | 4.3:-5173947 | MS.gene55755:CDS |
| AGTACTTATACGAAGATCAA+TGG | 0.434098 | 4.3:-5173746 | MS.gene55755:CDS |
| AGCTTGTGGACAATCAAGAT+TGG | 0.439651 | 4.3:-5173802 | MS.gene55755:CDS |
| ATATGTTCAAGAGGTCAATT+GGG | 0.448508 | 4.3:-5173706 | MS.gene55755:CDS |
| AATAGTGTTACCCTTATAAG+TGG | 0.474862 | 4.3:-5174037 | MS.gene55755:CDS |
| ATTCATTCACTTTCAGGTAT+GGG | 0.482613 | 4.3:-5173946 | MS.gene55755:CDS |
| TATAATGGAATTGATATTGG+AGG | 0.495203 | 4.3:-5173902 | MS.gene55755:CDS |
| AGTACATACGATATCCCAAC+AGG | 0.525073 | 4.3:-5173665 | MS.gene55755:CDS |
| AAAAGCTAAGGCGCAAAGAA+CGG | 0.530162 | 4.3:-5173861 | MS.gene55755:CDS |
| CACGTGTGATTGAACCTGTT+GGG | 0.532056 | 4.3:+5173651 | None:intergenic |
| TTCATTCACTTTCAGGTATG+GGG | 0.551722 | 4.3:-5173945 | MS.gene55755:CDS |
| AATGGAATTGATATTGGAGG+AGG | 0.560744 | 4.3:-5173899 | MS.gene55755:CDS |
| CTTATAAGTGGAAGTTTCAA+CGG | 0.570737 | 4.3:-5174025 | MS.gene55755:CDS |
| TGGAGGAGGAATACAACCAC+CGG | 0.577353 | 4.3:-5173885 | MS.gene55755:CDS |
| ACAACCACCGGGAAAAGCTA+AGG | 0.582230 | 4.3:-5173873 | MS.gene55755:CDS |
| AAATATTGAATATGTTCAAG+AGG | 0.596331 | 4.3:-5173715 | MS.gene55755:CDS |
| CCACGTGTGATTGAACCTGT+TGG | 0.609505 | 4.3:+5173650 | None:intergenic |
| CTAAGGCGCAAAGAACGGCA+AGG | 0.613584 | 4.3:-5173856 | MS.gene55755:CDS |
| GGCAAGGGTAAACGTGACAT+TGG | 0.620256 | 4.3:-5173840 | MS.gene55755:CDS |
| TAAGGCGCAAAGAACGGCAA+GGG | 0.636962 | 4.3:-5173855 | MS.gene55755:CDS |
| TATGTTCAAGAGGTCAATTG+GGG | 0.679448 | 4.3:-5173705 | MS.gene55755:CDS |
| GGAGGAGGAATACAACCACC+GGG | 0.691763 | 4.3:-5173884 | MS.gene55755:CDS |
| CCAACAGGTTCAATCACACG+TGG | 0.713111 | 4.3:-5173650 | MS.gene55755:CDS |
| AATTCCATTATAGAAAACAG+TGG | 0.718426 | 4.3:+5173913 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATATTGAATATGTTCAAG+AGG | - | chr4.3:5173944-5173963 | MS.gene55755:CDS | 20.0% |
| !!! | TTCTATAATGGAATTGATAT+TGG | - | chr4.3:5173754-5173773 | MS.gene55755:CDS | 20.0% |
| ! | AATTCCATTATAGAAAACAG+TGG | + | chr4.3:5173749-5173768 | None:intergenic | 25.0% |
| ! | ATACAAATTCATTCACTTTC+AGG | - | chr4.3:5173707-5173726 | MS.gene55755:CDS | 25.0% |
| !! | TATAATGGAATTGATATTGG+AGG | - | chr4.3:5173757-5173776 | MS.gene55755:CDS | 25.0% |
| AATAGTGTTACCCTTATAAG+TGG | - | chr4.3:5173622-5173641 | MS.gene55755:CDS | 30.0% | |
| AATATGTTCAAGAGGTCAAT+TGG | - | chr4.3:5173952-5173971 | MS.gene55755:CDS | 30.0% | |
| AGTACTTATACGAAGATCAA+TGG | - | chr4.3:5173913-5173932 | MS.gene55755:CDS | 30.0% | |
| ATATGTTCAAGAGGTCAATT+GGG | - | chr4.3:5173953-5173972 | MS.gene55755:CDS | 30.0% | |
| CTTATAAGTGGAAGTTTCAA+CGG | - | chr4.3:5173634-5173653 | MS.gene55755:CDS | 30.0% | |
| GTTGAAACTTCCACTTATAA+GGG | + | chr4.3:5173635-5173654 | None:intergenic | 30.0% | |
| ! | AACACCACTGTTTTCTATAA+TGG | - | chr4.3:5173742-5173761 | MS.gene55755:CDS | 30.0% |
| ! | AATTCATTCACTTTCAGGTA+TGG | - | chr4.3:5173712-5173731 | MS.gene55755:CDS | 30.0% |
| ! | ATTCATTCACTTTCAGGTAT+GGG | - | chr4.3:5173713-5173732 | MS.gene55755:CDS | 30.0% |
| AATGGAATTGATATTGGAGG+AGG | - | chr4.3:5173760-5173779 | MS.gene55755:CDS | 35.0% | |
| ACACGTGGTGATAATTGTAT+TGG | - | chr4.3:5174024-5174043 | MS.gene55755:CDS | 35.0% | |
| CGTTGAAACTTCCACTTATA+AGG | + | chr4.3:5173636-5173655 | None:intergenic | 35.0% | |
| GATTAATGCGAAAAAGCTTG+TGG | - | chr4.3:5173843-5173862 | MS.gene55755:CDS | 35.0% | |
| TATGTTCAAGAGGTCAATTG+GGG | - | chr4.3:5173954-5173973 | MS.gene55755:CDS | 35.0% | |
| ! | TTCATTCACTTTCAGGTATG+GGG | - | chr4.3:5173714-5173733 | MS.gene55755:CDS | 35.0% |
| AAAAGCTAAGGCGCAAAGAA+CGG | - | chr4.3:5173798-5173817 | MS.gene55755:CDS | 40.0% | |
| AGCTTGTGGACAATCAAGAT+TGG | - | chr4.3:5173857-5173876 | MS.gene55755:CDS | 40.0% | |
| AGTACATACGATATCCCAAC+AGG | - | chr4.3:5173994-5174013 | MS.gene55755:CDS | 40.0% | |
| CACGTGTGATTGAACCTGTT+GGG | + | chr4.3:5174011-5174030 | None:intergenic | 45.0% | |
| ACAACCACCGGGAAAAGCTA+AGG | - | chr4.3:5173786-5173805 | MS.gene55755:CDS | 50.0% | |
| CCAACAGGTTCAATCACACG+TGG | - | chr4.3:5174009-5174028 | MS.gene55755:CDS | 50.0% | |
| CCACGTGTGATTGAACCTGT+TGG | + | chr4.3:5174012-5174031 | None:intergenic | 50.0% | |
| GGCAAGGGTAAACGTGACAT+TGG | - | chr4.3:5173819-5173838 | MS.gene55755:CDS | 50.0% | |
| TAAGGCGCAAAGAACGGCAA+GGG | - | chr4.3:5173804-5173823 | MS.gene55755:CDS | 50.0% | |
| TGGAGGAGGAATACAACCAC+CGG | - | chr4.3:5173774-5173793 | MS.gene55755:CDS | 50.0% | |
| !! | CTTTGCGCCTTAGCTTTTCC+CGG | + | chr4.3:5173796-5173815 | None:intergenic | 50.0% |
| CTAAGGCGCAAAGAACGGCA+AGG | - | chr4.3:5173803-5173822 | MS.gene55755:CDS | 55.0% | |
| GGAGGAGGAATACAACCACC+GGG | - | chr4.3:5173775-5173794 | MS.gene55755:CDS | 55.0% | |
| !! | TGCGCCTTAGCTTTTCCCGG+TGG | + | chr4.3:5173793-5173812 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 5173619 | 5174062 | 5173619 | ID=MS.gene55755 |
| chr4.3 | mRNA | 5173619 | 5174062 | 5173619 | ID=MS.gene55755.t1;Parent=MS.gene55755 |
| chr4.3 | exon | 5173619 | 5174062 | 5173619 | ID=MS.gene55755.t1.exon1;Parent=MS.gene55755.t1 |
| chr4.3 | CDS | 5173619 | 5174062 | 5173619 | ID=cds.MS.gene55755.t1;Parent=MS.gene55755.t1 |
| Gene Sequence |
| Protein sequence |
