Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55819.t1 | XP_013447227.1 | 93.1 | 233 | 11 | 2 | 1 | 233 | 1 | 228 | 1.50E-115 | 425.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55819.t1 | A0A072TUQ1 | 93.1 | 233 | 11 | 2 | 1 | 233 | 1 | 228 | 1.0e-115 | 425.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052308 | MS.gene55819 | 0.814952 | 1.19E-51 | -1.69E-46 |
| MS.gene052367 | MS.gene55819 | 0.830321 | 3.14E-55 | -1.69E-46 |
| MS.gene052383 | MS.gene55819 | 0.81336 | 2.66E-51 | -1.69E-46 |
| MS.gene054593 | MS.gene55819 | 0.803056 | 4.18E-49 | -1.69E-46 |
| MS.gene055578 | MS.gene55819 | 0.80782 | 4.19E-50 | -1.69E-46 |
| MS.gene056041 | MS.gene55819 | 0.810382 | 1.18E-50 | -1.69E-46 |
| MS.gene05640 | MS.gene55819 | 0.81553 | 8.82E-52 | -1.69E-46 |
| MS.gene056526 | MS.gene55819 | 0.803158 | 3.98E-49 | -1.69E-46 |
| MS.gene056528 | MS.gene55819 | 0.818131 | 2.30E-52 | -1.69E-46 |
| MS.gene057367 | MS.gene55819 | 0.807119 | 5.91E-50 | -1.69E-46 |
| MS.gene057458 | MS.gene55819 | 0.803368 | 3.61E-49 | -1.69E-46 |
| MS.gene058128 | MS.gene55819 | 0.805692 | 1.18E-49 | -1.69E-46 |
| MS.gene058827 | MS.gene55819 | 0.809738 | 1.63E-50 | -1.69E-46 |
| MS.gene058828 | MS.gene55819 | 0.800792 | 1.22E-48 | -1.69E-46 |
| MS.gene058853 | MS.gene55819 | 0.824402 | 8.23E-54 | -1.69E-46 |
| MS.gene060473 | MS.gene55819 | 0.818008 | 2.45E-52 | -1.69E-46 |
| MS.gene060565 | MS.gene55819 | 0.816098 | 6.59E-52 | -1.69E-46 |
| MS.gene06093 | MS.gene55819 | 0.81328 | 2.77E-51 | -1.69E-46 |
| MS.gene061099 | MS.gene55819 | 0.81677 | 4.66E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55819.t1 | MTR_8g099655 | 93.133 | 233 | 11 | 2 | 1 | 233 | 1 | 228 | 2.43e-147 | 410 |
| MS.gene55819.t1 | MTR_8g017100 | 32.979 | 188 | 85 | 6 | 1 | 174 | 1 | 161 | 2.04e-19 | 83.2 |
| MS.gene55819.t1 | MTR_1g099630 | 27.363 | 201 | 128 | 5 | 1 | 193 | 1 | 191 | 2.90e-16 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 51 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGACTTGTGCAACTGTTT+TGG | 0.225939 | 4.3:-4383610 | None:intergenic |
| TGCTGCAGAAGGGTCAAATT+TGG | 0.265588 | 4.3:+4384178 | MS.gene55819:CDS |
| CTATTATCTTCTTCCCTTGA+AGG | 0.321987 | 4.3:+4383719 | MS.gene55819:CDS |
| CTCATGGTTCTGCTCCTTGT+AGG | 0.337212 | 4.3:-4384059 | None:intergenic |
| AATGGGAAATTGTTCTTTCA+AGG | 0.367226 | 4.3:+4383515 | None:intergenic |
| CTCGAAAATCGCCCGGGTTA+TGG | 0.367726 | 4.3:+4383630 | MS.gene55819:CDS |
| TTGTCAGAACAGGTAAATAC+TGG | 0.368907 | 4.3:+4383984 | MS.gene55819:CDS |
| TCAATAGAAAGAGTGCGATT+TGG | 0.399831 | 4.3:-4384152 | None:intergenic |
| CAGATGCAGGGTTGAAGAAT+AGG | 0.403928 | 4.3:+4383808 | MS.gene55819:CDS |
| TTCTTGGCGCCCGTTAGCTC+TGG | 0.404284 | 4.3:+4384122 | MS.gene55819:CDS |
| AAGCTCTCTTGGTCTGATAA+TGG | 0.421238 | 4.3:-4383681 | None:intergenic |
| ATCTGGTTGTACCTCCTTCA+AGG | 0.423314 | 4.3:-4383733 | None:intergenic |
| TCCCACTTTCCAAGTGCTCA+TGG | 0.456476 | 4.3:-4384075 | None:intergenic |
| GACCGTAGCTTAAGCTCTCT+TGG | 0.459574 | 4.3:-4383692 | None:intergenic |
| ATCAGAATTCTATGTGATAG+TGG | 0.460389 | 4.3:+4383564 | MS.gene55819:CDS |
| TGTTCTTTCAAGGGTACCAC+TGG | 0.470091 | 4.3:+4383525 | MS.gene55819:CDS |
| CACACACCATTCTTAGCTGA+TGG | 0.481472 | 4.3:-4383918 | None:intergenic |
| GGAATTCTTCCAGAGCTAAC+GGG | 0.486798 | 4.3:-4384131 | None:intergenic |
| ATGGTGCCGACACATGGCCT+TGG | 0.487730 | 4.3:-4383662 | None:intergenic |
| ACCATGAGCACTTGGAAAGT+GGG | 0.490347 | 4.3:+4384074 | MS.gene55819:CDS |
| TCATGGTTCTGCTCCTTGTA+GGG | 0.495277 | 4.3:-4384058 | None:intergenic |
| GGAGAATCTGTCGAATGGTT+CGG | 0.499420 | 4.3:+4383881 | MS.gene55819:CDS |
| ATGGGAAATTGTTCTTTCAA+GGG | 0.505584 | 4.3:+4383516 | MS.gene55819:CDS |
| AACCATGAGCACTTGGAAAG+TGG | 0.516348 | 4.3:+4384073 | MS.gene55819:CDS |
| TCTATTGATGATGCTGCAGA+AGG | 0.519843 | 4.3:+4384167 | MS.gene55819:CDS |
| CTGGTTCAAAGATCAGATGC+AGG | 0.526629 | 4.3:+4383795 | MS.gene55819:CDS |
| TGGAATTCTTCCAGAGCTAA+CGG | 0.528940 | 4.3:-4384132 | None:intergenic |
| TGGAGTTTCTGAACAAGAGC+TGG | 0.529828 | 4.3:+4383776 | MS.gene55819:CDS |
| TGGTGTGTGGAAAGTGAAGT+TGG | 0.537807 | 4.3:+4383932 | MS.gene55819:CDS |
| ACAAGTCCTCGAAAATCGCC+CGG | 0.550144 | 4.3:+4383623 | MS.gene55819:CDS |
| GGAGCAGAACCATGAGCACT+TGG | 0.558335 | 4.3:+4384066 | MS.gene55819:CDS |
| CAGAAAACTAGCTTTAGTGA+TGG | 0.569976 | 4.3:+4383756 | MS.gene55819:CDS |
| CTGATAATGGTGCCGACACA+TGG | 0.584425 | 4.3:-4383668 | None:intergenic |
| TTATCTTCTTCCCTTGAAGG+AGG | 0.590566 | 4.3:+4383722 | MS.gene55819:CDS |
| CAAGTCCTCGAAAATCGCCC+GGG | 0.599743 | 4.3:+4383624 | MS.gene55819:CDS |
| ATTCTATTAGTAGCCCTACA+AGG | 0.600271 | 4.3:+4384045 | MS.gene55819:CDS |
| TGATGAGATTGTAAAGAGCT+CGG | 0.605431 | 4.3:+4383833 | MS.gene55819:CDS |
| TGAGCACTTGGAAAGTGGGA+TGG | 0.605593 | 4.3:+4384078 | MS.gene55819:CDS |
| ATAGAATTCTGATAGAGTGA+TGG | 0.611014 | 4.3:-4383554 | None:intergenic |
| GAGCTTTGATTGAGAAGATG+AGG | 0.620197 | 4.3:+4384006 | MS.gene55819:CDS |
| GACCAAGAGAGCTTAAGCTA+CGG | 0.624125 | 4.3:+4383690 | MS.gene55819:CDS |
| GATTGATACAAGACAACTAG+AGG | 0.632915 | 4.3:+4383956 | MS.gene55819:CDS |
| TCTGGTTGTACCTCCTTCAA+GGG | 0.637312 | 4.3:-4383732 | None:intergenic |
| GTGTTGCCATCAGCTAAGAA+TGG | 0.640195 | 4.3:+4383912 | MS.gene55819:CDS |
| CTATTGATGATGCTGCAGAA+GGG | 0.641401 | 4.3:+4384168 | MS.gene55819:CDS |
| TGTTGCAGCGTGTGATTACG+TGG | 0.643926 | 4.3:+4383860 | MS.gene55819:CDS |
| TACGTGGAGAATCTGTCGAA+TGG | 0.644107 | 4.3:+4383876 | MS.gene55819:CDS |
| TGGTTCAAAGATCAGATGCA+GGG | 0.664540 | 4.3:+4383796 | MS.gene55819:CDS |
| TTTGATTGAGAAGATGAGGA+TGG | 0.666755 | 4.3:+4384010 | MS.gene55819:CDS |
| AGAGTGATGGCATTCTCCAG+TGG | 0.677222 | 4.3:-4383541 | None:intergenic |
| CATCAGCTAAGAATGGTGTG+TGG | 0.773073 | 4.3:+4383919 | MS.gene55819:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GGTTCAATTTTACAATTCAA+AGG | + | chr4.3:4383585-4383604 | MS.gene55819:CDS | 25.0% |
| !!! | AACGTTTTTCAAAACATTCT+TGG | + | chr4.3:4384106-4384125 | MS.gene55819:CDS | 25.0% |
| !!! | AAGAATGTTTTGAAAAACGT+TGG | - | chr4.3:4384107-4384126 | None:intergenic | 25.0% |
| ATAGAATTCTGATAGAGTGA+TGG | - | chr4.3:4383557-4383576 | None:intergenic | 30.0% | |
| ATCAGAATTCTATGTGATAG+TGG | + | chr4.3:4383564-4383583 | MS.gene55819:CDS | 30.0% | |
| ATGGGAAATTGTTCTTTCAA+GGG | + | chr4.3:4383516-4383535 | MS.gene55819:CDS | 30.0% | |
| ATTCTATTAGTAGCCCTACA+AGG | + | chr4.3:4384045-4384064 | MS.gene55819:CDS | 35.0% | |
| CTATTATCTTCTTCCCTTGA+AGG | + | chr4.3:4383719-4383738 | MS.gene55819:CDS | 35.0% | |
| GATTGATACAAGACAACTAG+AGG | + | chr4.3:4383956-4383975 | MS.gene55819:CDS | 35.0% | |
| TGATGAGATTGTAAAGAGCT+CGG | + | chr4.3:4383833-4383852 | MS.gene55819:CDS | 35.0% | |
| TTGTCAGAACAGGTAAATAC+TGG | + | chr4.3:4383984-4384003 | MS.gene55819:CDS | 35.0% | |
| TTTGATTGAGAAGATGAGGA+TGG | + | chr4.3:4384010-4384029 | MS.gene55819:CDS | 35.0% | |
| ! | TCAATAGAAAGAGTGCGATT+TGG | - | chr4.3:4384155-4384174 | None:intergenic | 35.0% |
| !! | CAGAAAACTAGCTTTAGTGA+TGG | + | chr4.3:4383756-4383775 | MS.gene55819:CDS | 35.0% |
| !!! | CTAAAGCTAGTTTTCTGATC+TGG | - | chr4.3:4383753-4383772 | None:intergenic | 35.0% |
| AAGCTCTCTTGGTCTGATAA+TGG | - | chr4.3:4383684-4383703 | None:intergenic | 40.0% | |
| TGGAATTCTTCCAGAGCTAA+CGG | - | chr4.3:4384135-4384154 | None:intergenic | 40.0% | |
| TGGTTCAAAGATCAGATGCA+GGG | + | chr4.3:4383796-4383815 | MS.gene55819:CDS | 40.0% | |
| TTATCTTCTTCCCTTGAAGG+AGG | + | chr4.3:4383722-4383741 | MS.gene55819:CDS | 40.0% | |
| ! | AGAGGAGATTTTGTCAGAAC+AGG | + | chr4.3:4383974-4383993 | MS.gene55819:CDS | 40.0% |
| ! | AGGACTTGTGCAACTGTTTT+GGG | - | chr4.3:4383612-4383631 | None:intergenic | 40.0% |
| ! | GAGCTTTGATTGAGAAGATG+AGG | + | chr4.3:4384006-4384025 | MS.gene55819:CDS | 40.0% |
| !! | CTATTGATGATGCTGCAGAA+GGG | + | chr4.3:4384168-4384187 | MS.gene55819:CDS | 40.0% |
| !! | TCTATTGATGATGCTGCAGA+AGG | + | chr4.3:4384167-4384186 | MS.gene55819:CDS | 40.0% |
| AACCATGAGCACTTGGAAAG+TGG | + | chr4.3:4384073-4384092 | MS.gene55819:CDS | 45.0% | |
| ACCATGAGCACTTGGAAAGT+GGG | + | chr4.3:4384074-4384093 | MS.gene55819:CDS | 45.0% | |
| ATCTGGTTGTACCTCCTTCA+AGG | - | chr4.3:4383736-4383755 | None:intergenic | 45.0% | |
| CACACACCATTCTTAGCTGA+TGG | - | chr4.3:4383921-4383940 | None:intergenic | 45.0% | |
| CAGATGCAGGGTTGAAGAAT+AGG | + | chr4.3:4383808-4383827 | MS.gene55819:CDS | 45.0% | |
| CATCAGCTAAGAATGGTGTG+TGG | + | chr4.3:4383919-4383938 | MS.gene55819:CDS | 45.0% | |
| CTGGTTCAAAGATCAGATGC+AGG | + | chr4.3:4383795-4383814 | MS.gene55819:CDS | 45.0% | |
| GACCAAGAGAGCTTAAGCTA+CGG | + | chr4.3:4383690-4383709 | MS.gene55819:CDS | 45.0% | |
| GGAATTCTTCCAGAGCTAAC+GGG | - | chr4.3:4384134-4384153 | None:intergenic | 45.0% | |
| GGAGAATCTGTCGAATGGTT+CGG | + | chr4.3:4383881-4383900 | MS.gene55819:CDS | 45.0% | |
| GTGTTGCCATCAGCTAAGAA+TGG | + | chr4.3:4383912-4383931 | MS.gene55819:CDS | 45.0% | |
| TACGTGGAGAATCTGTCGAA+TGG | + | chr4.3:4383876-4383895 | MS.gene55819:CDS | 45.0% | |
| TCTGGTTGTACCTCCTTCAA+GGG | - | chr4.3:4383735-4383754 | None:intergenic | 45.0% | |
| TGCTGCAGAAGGGTCAAATT+TGG | + | chr4.3:4384178-4384197 | MS.gene55819:CDS | 45.0% | |
| TGGAGTTTCTGAACAAGAGC+TGG | + | chr4.3:4383776-4383795 | MS.gene55819:CDS | 45.0% | |
| TGGCGAAAAACACCATAACC+CGG | - | chr4.3:4383645-4383664 | None:intergenic | 45.0% | |
| TGGTGTGTGGAAAGTGAAGT+TGG | + | chr4.3:4383932-4383951 | MS.gene55819:CDS | 45.0% | |
| ! | GAGGACTTGTGCAACTGTTT+TGG | - | chr4.3:4383613-4383632 | None:intergenic | 45.0% |
| ! | GGTTATGGTGTTTTTCGCCA+AGG | + | chr4.3:4383645-4383664 | MS.gene55819:CDS | 45.0% |
| ! | TCATGGTTCTGCTCCTTGTA+GGG | - | chr4.3:4384061-4384080 | None:intergenic | 45.0% |
| !! | TGTTCTTTCAAGGGTACCAC+TGG | + | chr4.3:4383525-4383544 | MS.gene55819:CDS | 45.0% |
| ACAAGTCCTCGAAAATCGCC+CGG | + | chr4.3:4383623-4383642 | MS.gene55819:CDS | 50.0% | |
| AGAGTGATGGCATTCTCCAG+TGG | - | chr4.3:4383544-4383563 | None:intergenic | 50.0% | |
| GACCGTAGCTTAAGCTCTCT+TGG | - | chr4.3:4383695-4383714 | None:intergenic | 50.0% | |
| GGCGAAAAACACCATAACCC+GGG | - | chr4.3:4383644-4383663 | None:intergenic | 50.0% | |
| TCCCACTTTCCAAGTGCTCA+TGG | - | chr4.3:4384078-4384097 | None:intergenic | 50.0% | |
| TGAGCACTTGGAAAGTGGGA+TGG | + | chr4.3:4384078-4384097 | MS.gene55819:CDS | 50.0% | |
| TGTTGCAGCGTGTGATTACG+TGG | + | chr4.3:4383860-4383879 | MS.gene55819:CDS | 50.0% | |
| ! | CTCATGGTTCTGCTCCTTGT+AGG | - | chr4.3:4384062-4384081 | None:intergenic | 50.0% |
| ! | TTTTCGCCAAGGCCATGTGT+CGG | + | chr4.3:4383656-4383675 | MS.gene55819:CDS | 50.0% |
| !! | CTGATAATGGTGCCGACACA+TGG | - | chr4.3:4383671-4383690 | None:intergenic | 50.0% |
| CAAGTCCTCGAAAATCGCCC+GGG | + | chr4.3:4383624-4383643 | MS.gene55819:CDS | 55.0% | |
| CTCGAAAATCGCCCGGGTTA+TGG | + | chr4.3:4383630-4383649 | MS.gene55819:CDS | 55.0% | |
| GGAGCAGAACCATGAGCACT+TGG | + | chr4.3:4384066-4384085 | MS.gene55819:CDS | 55.0% | |
| ! | CATAACCCGGGCGATTTTCG+AGG | - | chr4.3:4383632-4383651 | None:intergenic | 55.0% |
| TTCTTGGCGCCCGTTAGCTC+TGG | + | chr4.3:4384122-4384141 | MS.gene55819:CDS | 60.0% | |
| !! | ATGGTGCCGACACATGGCCT+TGG | - | chr4.3:4383665-4383684 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 4383516 | 4384217 | 4383516 | ID=MS.gene55819 |
| chr4.3 | mRNA | 4383516 | 4384217 | 4383516 | ID=MS.gene55819.t1;Parent=MS.gene55819 |
| chr4.3 | exon | 4383516 | 4384217 | 4383516 | ID=MS.gene55819.t1.exon1;Parent=MS.gene55819.t1 |
| chr4.3 | CDS | 4383516 | 4384217 | 4383516 | ID=cds.MS.gene55819.t1;Parent=MS.gene55819.t1 |
| Gene Sequence |
| Protein sequence |
