Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56491.t1 | RHN62028.1 | 84.5 | 84 | 13 | 0 | 4 | 87 | 73 | 156 | 2.80E-27 | 131 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56491.t1 | A0A396IB81 | 84.5 | 84 | 13 | 0 | 4 | 87 | 73 | 156 | 2.0e-27 | 131.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene56491 | 0.810204 | 1.29E-50 | -1.69E-46 |
| MS.gene049378 | MS.gene56491 | 0.874177 | 8.28E-68 | -1.69E-46 |
| MS.gene049669 | MS.gene56491 | 0.807646 | 4.57E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene56491 | 0.813895 | 2.03E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene56491 | 0.825736 | 3.98E-54 | -1.69E-46 |
| MS.gene050623 | MS.gene56491 | -0.813604 | 2.35E-51 | -1.69E-46 |
| MS.gene050642 | MS.gene56491 | 0.834654 | 2.64E-56 | -1.69E-46 |
| MS.gene050750 | MS.gene56491 | 0.815285 | 1.00E-51 | -1.69E-46 |
| MS.gene050953 | MS.gene56491 | 0.810767 | 9.79E-51 | -1.69E-46 |
| MS.gene051064 | MS.gene56491 | 0.817904 | 2.59E-52 | -1.69E-46 |
| MS.gene051452 | MS.gene56491 | 0.813268 | 2.79E-51 | -1.69E-46 |
| MS.gene051983 | MS.gene56491 | 0.827422 | 1.58E-54 | -1.69E-46 |
| MS.gene052077 | MS.gene56491 | 0.809524 | 1.81E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene56491 | 0.870599 | 1.29E-66 | -1.69E-46 |
| MS.gene052302 | MS.gene56491 | 0.814829 | 1.26E-51 | -1.69E-46 |
| MS.gene052309 | MS.gene56491 | -0.800793 | 1.22E-48 | -1.69E-46 |
| MS.gene052376 | MS.gene56491 | 0.829684 | 4.49E-55 | -1.69E-46 |
| MS.gene052389 | MS.gene56491 | 0.84139 | 4.89E-58 | -1.69E-46 |
| MS.gene054930 | MS.gene56491 | 0.8318 | 1.36E-55 | -1.69E-46 |
| MS.gene055701 | MS.gene56491 | 0.84075 | 7.21E-58 | -1.69E-46 |
| MS.gene056979 | MS.gene56491 | 0.815748 | 7.89E-52 | -1.69E-46 |
| MS.gene057026 | MS.gene56491 | 0.824608 | 7.36E-54 | -1.69E-46 |
| MS.gene05761 | MS.gene56491 | 0.825653 | 4.17E-54 | -1.69E-46 |
| MS.gene058500 | MS.gene56491 | 0.805362 | 1.39E-49 | -1.69E-46 |
| MS.gene060297 | MS.gene56491 | 0.804767 | 1.84E-49 | -1.69E-46 |
| MS.gene060572 | MS.gene56491 | 0.828211 | 1.02E-54 | -1.69E-46 |
| MS.gene060879 | MS.gene56491 | 0.824895 | 6.30E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56491.t1 | MTR_4g082883 | 51.316 | 76 | 31 | 2 | 16 | 87 | 26 | 99 | 8.08e-12 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACTTGGTAGAAACTTATT+TGG | 0.193374 | 4.4:+30145493 | None:intergenic |
| CACACCTGCTTCTTCGGTTT+TGG | 0.199931 | 4.4:+30145312 | None:intergenic |
| GTTTCTACCAAGTGAGGTTT+TGG | 0.234806 | 4.4:-30145485 | MS.gene56491:CDS |
| ATCAAGCACACCTGCTTCTT+CGG | 0.347454 | 4.4:+30145306 | None:intergenic |
| TTTGGCAGGCTCCACAGGTT+TGG | 0.367487 | 4.4:+30145330 | None:intergenic |
| CAGTTGTAGAGCAAATTGAT+AGG | 0.431190 | 4.4:-30145372 | MS.gene56491:CDS |
| GAAGAAGCAGGTGTGCTTGA+TGG | 0.500199 | 4.4:-30145304 | MS.gene56491:CDS |
| GCACTCCCCAAAACCTCACT+TGG | 0.541506 | 4.4:+30145478 | None:intergenic |
| CCTGCCAAAACCGAAGAAGC+AGG | 0.546448 | 4.4:-30145316 | MS.gene56491:CDS |
| AAATAAGTTTCTACCAAGTG+AGG | 0.567992 | 4.4:-30145491 | MS.gene56491:CDS |
| AGTTGTAGAGCAAATTGATA+GGG | 0.586465 | 4.4:-30145371 | MS.gene56491:CDS |
| TTATGTTCATTCCAAACCTG+TGG | 0.590330 | 4.4:-30145341 | MS.gene56491:CDS |
| TGTAGTGAAACAAGGTGAGA+AGG | 0.594496 | 4.4:-30145425 | MS.gene56491:CDS |
| AAGGTTGCAACTTCTGCAGA+GGG | 0.615194 | 4.4:-30145406 | MS.gene56491:CDS |
| CAAACAACTGTAGTGAAACA+AGG | 0.639740 | 4.4:-30145433 | MS.gene56491:CDS |
| GAAGGTTGCAACTTCTGCAG+AGG | 0.640521 | 4.4:-30145407 | MS.gene56491:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATAAGTTTCTACCAAGTG+AGG | - | chr4.4:30145290-30145309 | MS.gene56491:CDS | 30.0% | |
| AGTTGTAGAGCAAATTGATA+GGG | - | chr4.4:30145410-30145429 | MS.gene56491:CDS | 30.0% | |
| TCACTTGGTAGAAACTTATT+TGG | + | chr4.4:30145291-30145310 | None:intergenic | 30.0% | |
| CAAACAACTGTAGTGAAACA+AGG | - | chr4.4:30145348-30145367 | MS.gene56491:CDS | 35.0% | |
| CAGTTGTAGAGCAAATTGAT+AGG | - | chr4.4:30145409-30145428 | MS.gene56491:CDS | 35.0% | |
| TTATGTTCATTCCAAACCTG+TGG | - | chr4.4:30145440-30145459 | MS.gene56491:CDS | 35.0% | |
| ! | GATGGTGTTAAAAAGACTGT+TGG | - | chr4.4:30145495-30145514 | MS.gene56491:CDS | 35.0% |
| ! | TTTCTACCAAGTGAGGTTTT+GGG | - | chr4.4:30145297-30145316 | MS.gene56491:CDS | 35.0% |
| GGTGTTAAAAAGACTGTTGG+TGG | - | chr4.4:30145498-30145517 | MS.gene56491:CDS | 40.0% | |
| TGTAGTGAAACAAGGTGAGA+AGG | - | chr4.4:30145356-30145375 | MS.gene56491:CDS | 40.0% | |
| ! | GTTTCTACCAAGTGAGGTTT+TGG | - | chr4.4:30145296-30145315 | MS.gene56491:CDS | 40.0% |
| ! | TTCTACCAAGTGAGGTTTTG+GGG | - | chr4.4:30145298-30145317 | MS.gene56491:CDS | 40.0% |
| AAGGTTGCAACTTCTGCAGA+GGG | - | chr4.4:30145375-30145394 | MS.gene56491:CDS | 45.0% | |
| ATCAAGCACACCTGCTTCTT+CGG | + | chr4.4:30145478-30145497 | None:intergenic | 45.0% | |
| GAAGAAGCAGGTGTGCTTGA+TGG | - | chr4.4:30145477-30145496 | MS.gene56491:CDS | 50.0% | |
| GAAGGTTGCAACTTCTGCAG+AGG | - | chr4.4:30145374-30145393 | MS.gene56491:CDS | 50.0% | |
| ! | CACACCTGCTTCTTCGGTTT+TGG | + | chr4.4:30145472-30145491 | None:intergenic | 50.0% |
| !!! | TGAGGTTTTGGGGAGTGCAA+AGG | - | chr4.4:30145308-30145327 | MS.gene56491:CDS | 50.0% |
| CCTGCCAAAACCGAAGAAGC+AGG | - | chr4.4:30145465-30145484 | MS.gene56491:CDS | 55.0% | |
| GCACTCCCCAAAACCTCACT+TGG | + | chr4.4:30145306-30145325 | None:intergenic | 55.0% | |
| TTTGGCAGGCTCCACAGGTT+TGG | + | chr4.4:30145454-30145473 | None:intergenic | 55.0% | |
| !! | CCTGCTTCTTCGGTTTTGGC+AGG | + | chr4.4:30145468-30145487 | None:intergenic | 55.0% |
| !!! | GGTTTTGGGGAGTGCAAAGG+TGG | - | chr4.4:30145311-30145330 | MS.gene56491:CDS | 55.0% |
| !! | TCGGTTTTGGCAGGCTCCAC+AGG | + | chr4.4:30145459-30145478 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 30145270 | 30145533 | 30145270 | ID=MS.gene56491 |
| chr4.4 | mRNA | 30145270 | 30145533 | 30145270 | ID=MS.gene56491.t1;Parent=MS.gene56491 |
| chr4.4 | exon | 30145270 | 30145533 | 30145270 | ID=MS.gene56491.t1.exon1;Parent=MS.gene56491.t1 |
| chr4.4 | CDS | 30145270 | 30145533 | 30145270 | ID=cds.MS.gene56491.t1;Parent=MS.gene56491.t1 |
| Gene Sequence |
| Protein sequence |
