Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56493.t1 | XP_003607781.1 | 92.8 | 125 | 6 | 1 | 1 | 122 | 1 | 125 | 5.90E-52 | 213.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56493.t1 | Q9ZQ80 | 55.9 | 68 | 27 | 1 | 15 | 79 | 68 | 135 | 4.4e-11 | 68.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56493.t1 | G7JGC9 | 92.8 | 125 | 6 | 1 | 1 | 122 | 1 | 125 | 4.3e-52 | 213.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049438 | MS.gene56493 | 0.819173 | 1.33E-52 | -1.69E-46 |
| MS.gene04991 | MS.gene56493 | -0.811928 | 5.48E-51 | -1.69E-46 |
| MS.gene050762 | MS.gene56493 | 0.806314 | 8.73E-50 | -1.69E-46 |
| MS.gene050763 | MS.gene56493 | 0.806206 | 9.21E-50 | -1.69E-46 |
| MS.gene052309 | MS.gene56493 | -0.801287 | 9.67E-49 | -1.69E-46 |
| MS.gene054379 | MS.gene56493 | 0.824046 | 9.98E-54 | -1.69E-46 |
| MS.gene054930 | MS.gene56493 | 0.812782 | 3.57E-51 | -1.69E-46 |
| MS.gene055174 | MS.gene56493 | 0.80025 | 1.57E-48 | -1.69E-46 |
| MS.gene055441 | MS.gene56493 | 0.816481 | 5.41E-52 | -1.69E-46 |
| MS.gene056752 | MS.gene56493 | 0.801699 | 7.96E-49 | -1.69E-46 |
| MS.gene056849 | MS.gene56493 | 0.833126 | 6.38E-56 | -1.69E-46 |
| MS.gene056975 | MS.gene56493 | 0.808088 | 3.68E-50 | -1.69E-46 |
| MS.gene057451 | MS.gene56493 | -0.834562 | 2.79E-56 | -1.69E-46 |
| MS.gene057452 | MS.gene56493 | -0.821619 | 3.67E-53 | -1.69E-46 |
| MS.gene059872 | MS.gene56493 | -0.817235 | 3.66E-52 | -1.69E-46 |
| MS.gene060084 | MS.gene56493 | 0.822442 | 2.36E-53 | -1.69E-46 |
| MS.gene060297 | MS.gene56493 | 0.830592 | 2.69E-55 | -1.69E-46 |
| MS.gene060869 | MS.gene56493 | 0.823969 | 1.04E-53 | -1.69E-46 |
| MS.gene061004 | MS.gene56493 | 0.814598 | 1.42E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56493.t1 | MTR_4g082860 | 92.000 | 125 | 7 | 1 | 1 | 122 | 1 | 125 | 8.67e-69 | 202 |
| MS.gene56493.t1 | MTR_4g082830 | 65.909 | 132 | 32 | 4 | 3 | 122 | 11 | 141 | 6.76e-40 | 130 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56493.t1 | AT2G03440 | 42.636 | 129 | 52 | 5 | 11 | 122 | 64 | 187 | 4.22e-14 | 65.5 |
| MS.gene56493.t1 | AT1G13930 | 45.736 | 129 | 50 | 7 | 8 | 122 | 33 | 155 | 6.66e-13 | 61.6 |
| MS.gene56493.t1 | AT1G13930 | 45.736 | 129 | 50 | 7 | 8 | 122 | 33 | 155 | 6.66e-13 | 61.6 |
| MS.gene56493.t1 | AT1G13930 | 45.736 | 129 | 50 | 7 | 8 | 122 | 33 | 155 | 6.66e-13 | 61.6 |
Find 24 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGCAGCACAGTCAGGTTT+TGG | 0.225066 | 4.4:-30153953 | MS.gene56493:CDS |
| CCACCTGATCCTCCACCTTC+AGG | 0.341637 | 4.4:+30153722 | None:intergenic |
| AGTTGGTCAGTATGCTAAAT+TGG | 0.398975 | 4.4:-30153876 | MS.gene56493:CDS |
| TGCACTTGCCATTAGCTCAC+TGG | 0.413536 | 4.4:+30153988 | None:intergenic |
| GATTTCAACCAGTGAGCTAA+TGG | 0.416458 | 4.4:-30153996 | MS.gene56493:CDS |
| AAGCCTGAAGGTGGAGGATC+AGG | 0.423090 | 4.4:-30153725 | MS.gene56493:CDS |
| AGCTGCTGATTATCTGCATA+AGG | 0.469713 | 4.4:-30153819 | MS.gene56493:CDS |
| GGTGGAGGATCAGGTGGACT+AGG | 0.476662 | 4.4:-30153716 | MS.gene56493:CDS |
| GTAGCAGAGGCAGCACAGTC+AGG | 0.527765 | 4.4:-30153959 | MS.gene56493:CDS |
| TGATCTGCAGGTTTAGAAGC+TGG | 0.532370 | 4.4:+30153770 | None:intergenic |
| GACAAGACTGCAGAAGCTGC+TGG | 0.537867 | 4.4:-30153914 | MS.gene56493:CDS |
| AAGGTTGAGAACACTACTAC+TGG | 0.541196 | 4.4:-30153800 | MS.gene56493:CDS |
| AAGTGCAAAGGTGGTAGCAG+AGG | 0.549500 | 4.4:-30153972 | MS.gene56493:CDS |
| TTGGATGAACAGAAAGGTGT+AGG | 0.558648 | 4.4:-30153857 | MS.gene56493:CDS |
| GCTAAATTGGATGAACAGAA+AGG | 0.567227 | 4.4:-30153863 | MS.gene56493:CDS |
| GGTGATCTTCTAGATGCAGT+TGG | 0.590308 | 4.4:-30153893 | MS.gene56493:CDS |
| CCTGAAGGTGGAGGATCAGG+TGG | 0.601078 | 4.4:-30153722 | MS.gene56493:CDS |
| TGAGCTAATGGCAAGTGCAA+AGG | 0.603559 | 4.4:-30153984 | MS.gene56493:CDS |
| GGAGGATCAGGTGGACTAGG+TGG | 0.611533 | 4.4:-30153713 | MS.gene56493:CDS |
| GCTAATGGCAAGTGCAAAGG+TGG | 0.615992 | 4.4:-30153981 | MS.gene56493:CDS |
| GTTGCTAAGCCTGAAGGTGG+AGG | 0.637775 | 4.4:-30153731 | MS.gene56493:CDS |
| TCTGCAGGTTTAGAAGCTGG+TGG | 0.662750 | 4.4:+30153773 | None:intergenic |
| GATGATGTTGCTAAGCCTGA+AGG | 0.667542 | 4.4:-30153737 | MS.gene56493:CDS |
| GATGTTGCTAAGCCTGAAGG+TGG | 0.674394 | 4.4:-30153734 | MS.gene56493:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGCAACATCATCACTTTT+AGG | + | chr4.4:30153955-30153974 | None:intergenic | 30.0% |
| AGTTGGTCAGTATGCTAAAT+TGG | - | chr4.4:30153822-30153841 | MS.gene56493:CDS | 35.0% | |
| GCTAAATTGGATGAACAGAA+AGG | - | chr4.4:30153835-30153854 | MS.gene56493:CDS | 35.0% | |
| AAGGTTGAGAACACTACTAC+TGG | - | chr4.4:30153898-30153917 | MS.gene56493:CDS | 40.0% | |
| GATTTCAACCAGTGAGCTAA+TGG | - | chr4.4:30153702-30153721 | MS.gene56493:CDS | 40.0% | |
| ! | ACTAGGTGGTGATTTTGCTA+AGG | - | chr4.4:30153999-30154018 | MS.gene56493:CDS | 40.0% |
| ! | AGCTGCTGATTATCTGCATA+AGG | - | chr4.4:30153879-30153898 | MS.gene56493:CDS | 40.0% |
| ! | TTGGATGAACAGAAAGGTGT+AGG | - | chr4.4:30153841-30153860 | MS.gene56493:CDS | 40.0% |
| GATGATGTTGCTAAGCCTGA+AGG | - | chr4.4:30153961-30153980 | MS.gene56493:CDS | 45.0% | |
| GGTGATCTTCTAGATGCAGT+TGG | - | chr4.4:30153805-30153824 | MS.gene56493:CDS | 45.0% | |
| TGAGCTAATGGCAAGTGCAA+AGG | - | chr4.4:30153714-30153733 | MS.gene56493:CDS | 45.0% | |
| TGATCTGCAGGTTTAGAAGC+TGG | + | chr4.4:30153931-30153950 | None:intergenic | 45.0% | |
| ! | GATTTTGCTAAGGTTGCTGG+AGG | - | chr4.4:30154009-30154028 | MS.gene56493:CDS | 45.0% |
| ! | GGTGATTTTGCTAAGGTTGC+TGG | - | chr4.4:30154006-30154025 | MS.gene56493:CDS | 45.0% |
| !! | TCACTTTTAGGCTGATCTGC+AGG | + | chr4.4:30153943-30153962 | None:intergenic | 45.0% |
| AAGTGCAAAGGTGGTAGCAG+AGG | - | chr4.4:30153726-30153745 | MS.gene56493:CDS | 50.0% | |
| GATGTTGCTAAGCCTGAAGG+TGG | - | chr4.4:30153964-30153983 | MS.gene56493:CDS | 50.0% | |
| GCTAATGGCAAGTGCAAAGG+TGG | - | chr4.4:30153717-30153736 | MS.gene56493:CDS | 50.0% | |
| TCTGCAGGTTTAGAAGCTGG+TGG | + | chr4.4:30153928-30153947 | None:intergenic | 50.0% | |
| TGCACTTGCCATTAGCTCAC+TGG | + | chr4.4:30153713-30153732 | None:intergenic | 50.0% | |
| ! | AGGCAGCACAGTCAGGTTTT+GGG | - | chr4.4:30153746-30153765 | MS.gene56493:CDS | 50.0% |
| AAGCCTGAAGGTGGAGGATC+AGG | - | chr4.4:30153973-30153992 | MS.gene56493:CDS | 55.0% | |
| GACAAGACTGCAGAAGCTGC+TGG | - | chr4.4:30153784-30153803 | MS.gene56493:CDS | 55.0% | |
| GTTGCTAAGCCTGAAGGTGG+AGG | - | chr4.4:30153967-30153986 | MS.gene56493:CDS | 55.0% | |
| ! | GAGGCAGCACAGTCAGGTTT+TGG | - | chr4.4:30153745-30153764 | MS.gene56493:CDS | 55.0% |
| CCACCTGATCCTCCACCTTC+AGG | + | chr4.4:30153979-30153998 | None:intergenic | 60.0% | |
| CCTGAAGGTGGAGGATCAGG+TGG | - | chr4.4:30153976-30153995 | MS.gene56493:CDS | 60.0% | |
| GTAGCAGAGGCAGCACAGTC+AGG | - | chr4.4:30153739-30153758 | MS.gene56493:CDS | 60.0% | |
| ! | GGTGGAGGATCAGGTGGACT+AGG | - | chr4.4:30153982-30154001 | MS.gene56493:CDS | 60.0% |
| !! | GGAGGATCAGGTGGACTAGG+TGG | - | chr4.4:30153985-30154004 | MS.gene56493:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 30153676 | 30154044 | 30153676 | ID=MS.gene56493 |
| chr4.4 | mRNA | 30153676 | 30154044 | 30153676 | ID=MS.gene56493.t1;Parent=MS.gene56493 |
| chr4.4 | exon | 30153676 | 30154044 | 30153676 | ID=MS.gene56493.t1.exon1;Parent=MS.gene56493.t1 |
| chr4.4 | CDS | 30153676 | 30154044 | 30153676 | ID=cds.MS.gene56493.t1;Parent=MS.gene56493.t1 |
| Gene Sequence |
| Protein sequence |
