Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57222.t1 | XP_003589026.1 | 96 | 531 | 14 | 1 | 1 | 531 | 1 | 524 | 2.10E-266 | 927.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57222.t1 | P48731 | 59.1 | 286 | 102 | 4 | 221 | 505 | 175 | 446 | 6.6e-80 | 299.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57222.t1 | G7IB93 | 96.0 | 531 | 14 | 1 | 1 | 531 | 1 | 524 | 1.5e-266 | 927.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene57222.t1 | TF | HB-BELL |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene57222 | 0.801598 | 8.35E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene57222 | 0.813216 | 2.86E-51 | -1.69E-46 |
| MS.gene050584 | MS.gene57222 | 0.826791 | 2.23E-54 | -1.69E-46 |
| MS.gene050807 | MS.gene57222 | 0.8225 | 2.29E-53 | -1.69E-46 |
| MS.gene050808 | MS.gene57222 | 0.801925 | 7.15E-49 | -1.69E-46 |
| MS.gene051626 | MS.gene57222 | 0.945505 | 2.69E-104 | -1.69E-46 |
| MS.gene051789 | MS.gene57222 | 0.887289 | 1.63E-72 | -1.69E-46 |
| MS.gene052302 | MS.gene57222 | 0.801994 | 6.92E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene57222 | 0.806514 | 7.93E-50 | -1.69E-46 |
| MS.gene052967 | MS.gene57222 | 0.86665 | 2.44E-65 | -1.69E-46 |
| MS.gene053074 | MS.gene57222 | 0.879294 | 1.40E-69 | -1.69E-46 |
| MS.gene053630 | MS.gene57222 | 0.81587 | 7.41E-52 | -1.69E-46 |
| MS.gene054184 | MS.gene57222 | 0.836582 | 8.60E-57 | -1.69E-46 |
| MS.gene054185 | MS.gene57222 | 0.841144 | 5.68E-58 | -1.69E-46 |
| MS.gene05456 | MS.gene57222 | 0.837552 | 4.86E-57 | -1.69E-46 |
| MS.gene054596 | MS.gene57222 | 0.840682 | 7.51E-58 | -1.69E-46 |
| MS.gene055133 | MS.gene57222 | 0.822346 | 2.49E-53 | -1.69E-46 |
| MS.gene055207 | MS.gene57222 | 0.835241 | 1.88E-56 | -1.69E-46 |
| MS.gene056069 | MS.gene57222 | -0.804354 | 2.25E-49 | -1.69E-46 |
| MS.gene057754 | MS.gene57222 | 0.806883 | 6.62E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene57222 | 0.830338 | 3.11E-55 | -1.69E-46 |
| MS.gene058906 | MS.gene57222 | 0.817017 | 4.10E-52 | -1.69E-46 |
| MS.gene059006 | MS.gene57222 | 0.877496 | 6.00E-69 | -1.69E-46 |
| MS.gene060476 | MS.gene57222 | 0.847121 | 1.41E-59 | -1.69E-46 |
| MS.gene061113 | MS.gene57222 | 0.806829 | 6.80E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene034624 | MS.gene57222 | PPI |
| MS.gene39202 | MS.gene57222 | PPI |
| MS.gene46486 | MS.gene57222 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57222.t1 | MTR_1g016490 | 96.987 | 531 | 9 | 1 | 1 | 531 | 1 | 524 | 0.0 | 1059 |
| MS.gene57222.t1 | MTR_4g051532 | 35.739 | 291 | 157 | 7 | 250 | 523 | 217 | 494 | 9.30e-48 | 177 |
| MS.gene57222.t1 | MTR_3g112300 | 38.462 | 260 | 150 | 5 | 247 | 500 | 159 | 414 | 2.31e-44 | 164 |
| MS.gene57222.t1 | MTR_3g112300 | 38.462 | 260 | 150 | 5 | 247 | 500 | 159 | 414 | 4.68e-44 | 164 |
| MS.gene57222.t1 | MTR_1g023050 | 37.548 | 261 | 144 | 5 | 252 | 500 | 172 | 425 | 1.22e-43 | 164 |
| MS.gene57222.t1 | MTR_7g106320 | 43.005 | 193 | 96 | 4 | 318 | 500 | 239 | 427 | 7.19e-43 | 162 |
| MS.gene57222.t1 | MTR_8g078480 | 38.739 | 222 | 120 | 5 | 306 | 517 | 447 | 662 | 1.16e-41 | 160 |
| MS.gene57222.t1 | MTR_1g057790 | 35.915 | 284 | 152 | 5 | 251 | 517 | 162 | 432 | 3.83e-41 | 157 |
| MS.gene57222.t1 | MTR_3g112290 | 36.842 | 266 | 146 | 7 | 247 | 500 | 162 | 417 | 8.04e-41 | 153 |
| MS.gene57222.t1 | MTR_5g018860 | 38.739 | 222 | 117 | 6 | 306 | 518 | 394 | 605 | 3.75e-40 | 155 |
| MS.gene57222.t1 | MTR_8g098815 | 41.899 | 179 | 95 | 4 | 325 | 494 | 254 | 432 | 8.23e-39 | 150 |
| MS.gene57222.t1 | MTR_4g019450 | 31.541 | 279 | 167 | 7 | 247 | 522 | 193 | 450 | 5.02e-37 | 145 |
| MS.gene57222.t1 | MTR_7g065050 | 37.624 | 202 | 117 | 5 | 329 | 525 | 200 | 397 | 2.55e-36 | 142 |
| MS.gene57222.t1 | MTR_2g095050 | 36.923 | 195 | 105 | 3 | 322 | 498 | 312 | 506 | 1.27e-33 | 135 |
| MS.gene57222.t1 | MTR_3g112300 | 34.874 | 238 | 145 | 5 | 247 | 478 | 159 | 392 | 2.04e-29 | 120 |
| MS.gene57222.t1 | MTR_3g112300 | 31.797 | 217 | 138 | 5 | 247 | 457 | 159 | 371 | 1.30e-19 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57222.t1 | AT4G32980 | 58.228 | 316 | 110 | 6 | 191 | 505 | 152 | 446 | 5.65e-110 | 336 |
| MS.gene57222.t1 | AT4G32980 | 58.228 | 316 | 110 | 6 | 191 | 505 | 152 | 446 | 5.65e-110 | 336 |
| MS.gene57222.t1 | AT2G16400 | 36.918 | 279 | 149 | 7 | 247 | 517 | 111 | 370 | 2.88e-43 | 160 |
| MS.gene57222.t1 | AT2G27220 | 41.622 | 185 | 82 | 5 | 324 | 500 | 131 | 297 | 4.61e-42 | 156 |
| MS.gene57222.t1 | AT2G27220 | 41.622 | 185 | 82 | 5 | 324 | 500 | 131 | 297 | 4.61e-42 | 156 |
| MS.gene57222.t1 | AT1G19700 | 34.799 | 273 | 145 | 7 | 247 | 500 | 162 | 420 | 7.39e-42 | 157 |
| MS.gene57222.t1 | AT1G19700 | 34.799 | 273 | 145 | 7 | 247 | 500 | 162 | 420 | 7.39e-42 | 157 |
| MS.gene57222.t1 | AT1G19700 | 34.799 | 273 | 145 | 7 | 247 | 500 | 162 | 420 | 7.39e-42 | 157 |
| MS.gene57222.t1 | AT1G19700 | 34.799 | 273 | 145 | 7 | 247 | 500 | 162 | 420 | 7.39e-42 | 157 |
| MS.gene57222.t1 | AT1G19700 | 34.799 | 273 | 145 | 7 | 247 | 500 | 162 | 420 | 7.39e-42 | 157 |
| MS.gene57222.t1 | AT4G34610 | 32.873 | 362 | 199 | 12 | 149 | 500 | 56 | 383 | 1.36e-41 | 157 |
| MS.gene57222.t1 | AT4G34610 | 32.873 | 362 | 199 | 12 | 149 | 500 | 56 | 383 | 1.36e-41 | 157 |
| MS.gene57222.t1 | AT4G34610 | 32.873 | 362 | 199 | 12 | 149 | 500 | 56 | 383 | 1.36e-41 | 157 |
| MS.gene57222.t1 | AT4G34610 | 32.873 | 362 | 199 | 12 | 149 | 500 | 56 | 383 | 1.36e-41 | 157 |
| MS.gene57222.t1 | AT1G75410 | 35.632 | 261 | 152 | 6 | 247 | 500 | 164 | 415 | 2.22e-41 | 156 |
| MS.gene57222.t1 | AT1G75410 | 35.632 | 261 | 152 | 6 | 247 | 500 | 164 | 415 | 2.22e-41 | 156 |
| MS.gene57222.t1 | AT2G27220 | 42.941 | 170 | 71 | 5 | 339 | 500 | 164 | 315 | 1.74e-38 | 147 |
| MS.gene57222.t1 | AT1G75430 | 40.314 | 191 | 99 | 3 | 323 | 500 | 83 | 271 | 5.13e-38 | 141 |
| MS.gene57222.t1 | AT4G36870 | 36.538 | 208 | 114 | 4 | 305 | 500 | 366 | 567 | 2.34e-37 | 147 |
| MS.gene57222.t1 | AT4G36870 | 36.538 | 208 | 114 | 4 | 305 | 500 | 366 | 567 | 2.34e-37 | 147 |
| MS.gene57222.t1 | AT2G23760 | 37.255 | 204 | 110 | 4 | 307 | 498 | 294 | 491 | 4.42e-36 | 142 |
| MS.gene57222.t1 | AT2G23760 | 37.255 | 204 | 110 | 4 | 307 | 498 | 294 | 491 | 4.42e-36 | 142 |
| MS.gene57222.t1 | AT2G23760 | 37.255 | 204 | 110 | 4 | 307 | 498 | 294 | 491 | 4.42e-36 | 142 |
| MS.gene57222.t1 | AT5G41410 | 39.899 | 198 | 105 | 5 | 319 | 503 | 267 | 463 | 2.03e-35 | 140 |
| MS.gene57222.t1 | AT2G27990 | 40.462 | 173 | 99 | 3 | 328 | 499 | 323 | 492 | 1.52e-34 | 137 |
| MS.gene57222.t1 | AT2G35940 | 29.064 | 406 | 239 | 10 | 126 | 492 | 51 | 446 | 6.02e-34 | 136 |
| MS.gene57222.t1 | AT2G35940 | 29.064 | 406 | 239 | 10 | 126 | 492 | 51 | 446 | 6.02e-34 | 136 |
| MS.gene57222.t1 | AT2G35940 | 29.064 | 406 | 239 | 10 | 126 | 492 | 51 | 446 | 6.02e-34 | 136 |
| MS.gene57222.t1 | AT5G02030 | 36.813 | 182 | 100 | 3 | 332 | 498 | 234 | 415 | 1.03e-29 | 123 |
| MS.gene57222.t1 | AT2G23760 | 29.688 | 256 | 110 | 5 | 307 | 498 | 294 | 543 | 1.23e-27 | 117 |
| MS.gene57222.t1 | AT1G75410 | 30.962 | 239 | 149 | 6 | 247 | 478 | 164 | 393 | 7.98e-26 | 110 |
| MS.gene57222.t1 | AT4G36870 | 31.551 | 187 | 110 | 4 | 305 | 479 | 366 | 546 | 1.05e-22 | 102 |
| MS.gene57222.t1 | AT4G36870 | 30.811 | 185 | 110 | 4 | 305 | 477 | 366 | 544 | 1.06e-21 | 99.0 |
| MS.gene57222.t1 | AT1G75430 | 31.081 | 148 | 87 | 3 | 323 | 457 | 83 | 228 | 1.66e-13 | 71.6 |
| MS.gene57222.t1 | AT2G27990 | 32.824 | 131 | 84 | 3 | 328 | 457 | 323 | 450 | 8.95e-13 | 70.9 |
Find 90 sgRNAs with CRISPR-Local
Find 155 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCATACATATTCAATAAT+TGG | 0.188620 | 1.4:-5912950 | MS.gene57222:CDS |
| AATGGAATTCCAATACTTTC+TGG | 0.223215 | 1.4:-5913155 | MS.gene57222:CDS |
| ATCTTTCTGGCAAGCCCCTT+TGG | 0.227378 | 1.4:+5911362 | None:intergenic |
| AACCAAAACAACATCATAAA+TGG | 0.230798 | 1.4:-5913173 | MS.gene57222:CDS |
| ATGGAACTTTCTATGAGTTT+AGG | 0.232674 | 1.4:-5912597 | MS.gene57222:CDS |
| TCTTTCTGGCAAGCCCCTTT+GGG | 0.243439 | 1.4:+5911363 | None:intergenic |
| AAATGCTTCTCTGCATCTTT+AGG | 0.274049 | 1.4:+5911055 | None:intergenic |
| TCTTGATGAGATAAACTATT+CGG | 0.288114 | 1.4:-5912472 | MS.gene57222:CDS |
| TTCCATTTATGATGTTGTTT+TGG | 0.288436 | 1.4:+5913171 | None:intergenic |
| TGGAACTTTCTATGAGTTTA+GGG | 0.292728 | 1.4:-5912596 | MS.gene57222:CDS |
| AACGGTTTCTCGACTATATA+AGG | 0.297571 | 1.4:-5911542 | MS.gene57222:CDS |
| CTGTTTCCGTTCTACGTGAT+TGG | 0.305984 | 1.4:-5911339 | MS.gene57222:CDS |
| AACCCCTTGTGTGCAACTTT+TGG | 0.320504 | 1.4:-5912909 | MS.gene57222:CDS |
| TATTCGGGTTCTGGTGTTAG+AGG | 0.338538 | 1.4:-5912456 | MS.gene57222:CDS |
| GAGATAAACTATTCGGGTTC+TGG | 0.339602 | 1.4:-5912465 | MS.gene57222:CDS |
| ACAAGCCTCATGTAGTAGTA+TGG | 0.375487 | 1.4:-5912616 | MS.gene57222:CDS |
| GAATGGAACAAGATCGGGTT+TGG | 0.377735 | 1.4:-5912640 | MS.gene57222:CDS |
| TTTGATGATTGCCATCCATT+TGG | 0.383458 | 1.4:+5912792 | None:intergenic |
| AAGATACCTTGCTGTAATTC+AGG | 0.388861 | 1.4:-5912532 | MS.gene57222:CDS |
| GGTTTATAAATGCGCGTGTT+CGG | 0.404462 | 1.4:-5910666 | MS.gene57222:CDS |
| AAGTTCATTGCTACAATTTG+AGG | 0.409458 | 1.4:+5912737 | None:intergenic |
| CTTGATGAGATAAACTATTC+GGG | 0.413238 | 1.4:-5912471 | MS.gene57222:CDS |
| AGAACGGAAACAGATCTTTC+TGG | 0.415143 | 1.4:+5911349 | None:intergenic |
| TAAATGCGCGTGTTCGGCTA+TGG | 0.423300 | 1.4:-5910660 | MS.gene57222:CDS |
| TTCATTCATTCAAAAGCAAT+GGG | 0.425095 | 1.4:-5911425 | MS.gene57222:CDS |
| TATCTTGATCCTAATATTGC+CGG | 0.425897 | 1.4:+5912549 | None:intergenic |
| TGCCAATTATTGAATATGTA+TGG | 0.427184 | 1.4:+5912948 | None:intergenic |
| GCATACATTTCCTCAATCAA+TGG | 0.431572 | 1.4:+5910634 | None:intergenic |
| AAATGTTTAATATGAATTGA+AGG | 0.435021 | 1.4:-5910531 | None:intergenic |
| CTTGCAACATCACCAACTTC+AGG | 0.442716 | 1.4:-5912705 | MS.gene57222:CDS |
| TAGCAAACATATTCTTTCCA+TGG | 0.443732 | 1.4:-5911503 | MS.gene57222:CDS |
| GGGCTCTTCAACAGTTGAAA+AGG | 0.453231 | 1.4:-5911405 | MS.gene57222:CDS |
| CAGTCAATATAGTCAATGTT+TGG | 0.454449 | 1.4:-5911644 | MS.gene57222:CDS |
| TTATTAACAATGAGATTTGA+GGG | 0.454533 | 1.4:+5913029 | None:intergenic |
| TGCACACTTTGCTGTCAAAA+CGG | 0.454758 | 1.4:-5911560 | MS.gene57222:CDS |
| TGAAAAGGAAAGATCAGTTA+TGG | 0.458634 | 1.4:-5911390 | MS.gene57222:CDS |
| GTTATTAACAATGAGATTTG+AGG | 0.462088 | 1.4:+5913028 | None:intergenic |
| CTTCATTCATTCAAAAGCAA+TGG | 0.463764 | 1.4:-5911426 | MS.gene57222:CDS |
| TGTTCTCCACCAGAAAGTAT+TGG | 0.466176 | 1.4:+5913146 | None:intergenic |
| GCAAAGTGTGCATGTATTTG+TGG | 0.466187 | 1.4:+5911571 | None:intergenic |
| AGCATGAATGGAACAAGATC+GGG | 0.483225 | 1.4:-5912645 | MS.gene57222:CDS |
| AAAGCTCAAGAGAGTAATGT+TGG | 0.486264 | 1.4:-5912855 | MS.gene57222:CDS |
| ATTTATTCCTATCCTTCGCT+TGG | 0.489525 | 1.4:+5912401 | None:intergenic |
| TTCATGGTCAGAAGAGGATA+AGG | 0.491594 | 1.4:-5911464 | MS.gene57222:CDS |
| AGCAAACATATTCTTTCCAT+GGG | 0.494717 | 1.4:-5911502 | MS.gene57222:CDS |
| ACTGTTAAAATTTGATCCCA+TGG | 0.497477 | 1.4:+5911486 | None:intergenic |
| GACTTAACTCATAGCATGAA+TGG | 0.499550 | 1.4:-5912657 | MS.gene57222:CDS |
| ATGAGAGCAGTGAAAGAAGC+AGG | 0.499804 | 1.4:-5910570 | MS.gene57222:CDS |
| GGTTCTGGTGTTAGAGGCCG+AGG | 0.503924 | 1.4:-5912450 | MS.gene57222:CDS |
| TGGTTGTGTAGGTATCAAAC+TGG | 0.504145 | 1.4:-5910687 | MS.gene57222:intron |
| AAGTGGGTTGACTAGAAGTC+AGG | 0.504398 | 1.4:-5911020 | MS.gene57222:intron |
| AACAAATGAATTATCAGCTA+TGG | 0.505083 | 1.4:+5913085 | None:intergenic |
| TCTTGAAGACCAATTCTAGC+AGG | 0.505334 | 1.4:+5912990 | None:intergenic |
| AGAAAAGCTTGTCGAAATGA+AGG | 0.508701 | 1.4:-5910598 | MS.gene57222:CDS |
| ATCAGTTATGGAGGCCCCAA+AGG | 0.509828 | 1.4:-5911378 | MS.gene57222:CDS |
| CTTTCTGGCAAGCCCCTTTG+GGG | 0.514861 | 1.4:+5911364 | None:intergenic |
| TCAGTTATGGAGGCCCCAAA+GGG | 0.515348 | 1.4:-5911377 | MS.gene57222:CDS |
| TCAGCATAAGCTTCTGACAT+AGG | 0.527852 | 1.4:+5912366 | None:intergenic |
| TCATGTCATATTCGCCGATA+GGG | 0.529853 | 1.4:-5912824 | MS.gene57222:CDS |
| ATAGGGAACATCGATCCAAA+TGG | 0.530007 | 1.4:-5912807 | MS.gene57222:CDS |
| TAGCATGAATGGAACAAGAT+CGG | 0.530199 | 1.4:-5912646 | MS.gene57222:CDS |
| ACAAAGTTGATTTATTTCCT+CGG | 0.530630 | 1.4:+5912433 | None:intergenic |
| GGTCAATGCTCAGAGATTAG+TGG | 0.536668 | 1.4:-5912684 | MS.gene57222:CDS |
| ATGCTGATTCTTCATTGCAA+AGG | 0.542693 | 1.4:-5912350 | MS.gene57222:CDS |
| GCTATGGAAACCATTGATTG+AGG | 0.555071 | 1.4:-5910644 | MS.gene57222:CDS |
| ATCACCAAAAGTTGCACACA+AGG | 0.561105 | 1.4:+5912905 | None:intergenic |
| TCTGAGCATTGACCTGAAGT+TGG | 0.571861 | 1.4:+5912693 | None:intergenic |
| CACCAAAAGTTGCACACAAG+GGG | 0.572833 | 1.4:+5912907 | None:intergenic |
| TAACAGTTCATGGTCAGAAG+AGG | 0.576067 | 1.4:-5911470 | MS.gene57222:CDS |
| GGAATTCCAATACTTTCTGG+TGG | 0.576914 | 1.4:-5913152 | MS.gene57222:CDS |
| ACTCTTGATGCTTCTACAGA+TGG | 0.577737 | 1.4:-5912301 | MS.gene57222:intron |
| ACTGCTCAAATGAACATGGC+TGG | 0.584941 | 1.4:-5913275 | MS.gene57222:CDS |
| AACACAACCAAGCGAAGGAT+AGG | 0.588145 | 1.4:-5912408 | MS.gene57222:CDS |
| TTGTTACATCACAACATGGA+AGG | 0.589701 | 1.4:-5913067 | MS.gene57222:CDS |
| AATGAATAGCAATGAATGAG+TGG | 0.594918 | 1.4:+5913206 | None:intergenic |
| AAACATCCAATCACGTAGAA+CGG | 0.600137 | 1.4:+5911333 | None:intergenic |
| TTCATGTCATATTCGCCGAT+AGG | 0.602007 | 1.4:-5912825 | MS.gene57222:CDS |
| ACAACATGGAAGGACTATTG+TGG | 0.614360 | 1.4:-5913057 | MS.gene57222:CDS |
| TTTGGATCGATGTTCCCTAT+CGG | 0.616890 | 1.4:+5912810 | None:intergenic |
| TAGATAATACTTACGGATGG+AGG | 0.617974 | 1.4:+5911302 | None:intergenic |
| TATATAAGGACTTGAGAGAG+AGG | 0.620065 | 1.4:-5911528 | MS.gene57222:CDS |
| AAGTTCCATACTACTACATG+AGG | 0.620943 | 1.4:+5912611 | None:intergenic |
| GGAACATCGATCCAAATGGA+TGG | 0.633164 | 1.4:-5912803 | MS.gene57222:CDS |
| TCACCAAAAGTTGCACACAA+GGG | 0.650888 | 1.4:+5912906 | None:intergenic |
| TCATTTGTTACATCACAACA+TGG | 0.664816 | 1.4:-5913071 | MS.gene57222:CDS |
| AAAGGAAAGATCAGTTATGG+AGG | 0.667219 | 1.4:-5911387 | MS.gene57222:CDS |
| TGTCGAACACAACCAAGCGA+AGG | 0.672572 | 1.4:-5912413 | MS.gene57222:CDS |
| GTACACTGCTCAAATGAACA+TGG | 0.673477 | 1.4:-5913279 | MS.gene57222:CDS |
| GTATTTCCTGAATTACAGCA+AGG | 0.683112 | 1.4:+5912526 | None:intergenic |
| CAGTTATGGAGGCCCCAAAG+GGG | 0.731077 | 1.4:-5911376 | MS.gene57222:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAACCAAAATTTTAATATAA+AGG | - | chr1.4:5912560-5912579 | MS.gene57222:CDS | 10.0% |
| !! | GATCTTATTATTATAGATAA+AGG | + | chr1.4:5912703-5912722 | None:intergenic | 15.0% |
| !! | TATCTATAATAATAAGATCA+TGG | - | chr1.4:5912704-5912723 | MS.gene57222:CDS | 15.0% |
| !! | TGAATTTAATGTAATCTAAA+TGG | + | chr1.4:5911563-5911582 | None:intergenic | 15.0% |
| !!! | AGACCTTTATATTAAAATTT+TGG | + | chr1.4:5912566-5912585 | None:intergenic | 15.0% |
| !!! | TAATAACTTTTATACGATAA+GGG | + | chr1.4:5913078-5913097 | None:intergenic | 15.0% |
| !!! | TTAATAACTTTTATACGATA+AGG | + | chr1.4:5913079-5913098 | None:intergenic | 15.0% |
| !! | AGTGATTAGAGTTATATTTA+TGG | - | chr1.4:5913116-5913135 | MS.gene57222:CDS | 20.0% |
| !! | ATTAGAGTTATATTTATGGT+TGG | - | chr1.4:5913120-5913139 | MS.gene57222:CDS | 20.0% |
| !! | ATTTACTTGCAATAAAAAGT+GGG | - | chr1.4:5912791-5912810 | MS.gene57222:CDS | 20.0% |
| !! | TCGATTATAGATAATACTTA+CGG | + | chr1.4:5912535-5912554 | None:intergenic | 20.0% |
| !! | TTATTAACAATGAGATTTGA+GGG | + | chr1.4:5910801-5910820 | None:intergenic | 20.0% |
| !! | TTCCATACATATTCAATAAT+TGG | - | chr1.4:5910877-5910896 | MS.gene57222:intron | 20.0% |
| !! | TTTCAACTGATAAATAATCT+TGG | + | chr1.4:5912976-5912995 | None:intergenic | 20.0% |
| !!! | ACTTTTCCAAGTAAAATTTA+TGG | - | chr1.4:5911939-5911958 | MS.gene57222:intron | 20.0% |
| !!! | CATGTAAATTGATTTATGTT+TGG | + | chr1.4:5911815-5911834 | None:intergenic | 20.0% |
| !!! | TTAAAACTGATTTTGGTATT+TGG | - | chr1.4:5912919-5912938 | MS.gene57222:CDS | 20.0% |
| ! | AACAAATGAATTATCAGCTA+TGG | + | chr1.4:5910745-5910764 | None:intergenic | 25.0% |
| ! | AACCAAAACAACATCATAAA+TGG | - | chr1.4:5910654-5910673 | MS.gene57222:CDS | 25.0% |
| ! | ATTTATCAGTTGAAAACATG+TGG | - | chr1.4:5912981-5913000 | MS.gene57222:CDS | 25.0% |
| ! | CATTTACTTGCAATAAAAAG+TGG | - | chr1.4:5912790-5912809 | MS.gene57222:CDS | 25.0% |
| ! | GTTATTAACAATGAGATTTG+AGG | + | chr1.4:5910802-5910821 | None:intergenic | 25.0% |
| ! | TCTTGATGAGATAAACTATT+CGG | - | chr1.4:5911355-5911374 | MS.gene57222:CDS | 25.0% |
| ! | TGCCAATTATTGAATATGTA+TGG | + | chr1.4:5910882-5910901 | None:intergenic | 25.0% |
| ! | TTATAGATAATACTTACGGA+TGG | + | chr1.4:5912531-5912550 | None:intergenic | 25.0% |
| ! | TTCATTCATTCAAAAGCAAT+GGG | - | chr1.4:5912402-5912421 | MS.gene57222:CDS | 25.0% |
| ! | TTCCAAGTAAAATTTATGGA+TGG | - | chr1.4:5911943-5911962 | MS.gene57222:intron | 25.0% |
| ! | TTGATTTCTGATGTTTAGAA+TGG | - | chr1.4:5911661-5911680 | MS.gene57222:intron | 25.0% |
| !! | AACCATCCATAAATTTTACT+TGG | + | chr1.4:5911948-5911967 | None:intergenic | 25.0% |
| !! | ACAAAGTTGATTTATTTCCT+CGG | + | chr1.4:5911397-5911416 | None:intergenic | 25.0% |
| !!! | AGAATGGATTTTGACATTTT+TGG | - | chr1.4:5911677-5911696 | MS.gene57222:intron | 25.0% |
| !!! | GATCAAATTTTAACAGTTCA+TGG | - | chr1.4:5912347-5912366 | MS.gene57222:CDS | 25.0% |
| !!! | GTCACATACATATTTTGATT+TGG | - | chr1.4:5912839-5912858 | MS.gene57222:CDS | 25.0% |
| !!! | TAGTAGTCTTTATTTTACTC+AGG | - | chr1.4:5912156-5912175 | MS.gene57222:intron | 25.0% |
| !!! | TTCCATTTATGATGTTGTTT+TGG | + | chr1.4:5910659-5910678 | None:intergenic | 25.0% |
| AAACATACACTAATTCGATC+AGG | + | chr1.4:5911613-5911632 | None:intergenic | 30.0% | |
| AAGTTCATTGCTACAATTTG+AGG | + | chr1.4:5911093-5911112 | None:intergenic | 30.0% | |
| AATGAATAGCAATGAATGAG+TGG | + | chr1.4:5910624-5910643 | None:intergenic | 30.0% | |
| AATGGAATTCCAATACTTTC+TGG | - | chr1.4:5910672-5910691 | MS.gene57222:CDS | 30.0% | |
| ACTGTTAAAATTTGATCCCA+TGG | + | chr1.4:5912344-5912363 | None:intergenic | 30.0% | |
| AGCAAACATATTCTTTCCAT+GGG | - | chr1.4:5912325-5912344 | MS.gene57222:CDS | 30.0% | |
| AGGCTAAAAATCAATTGTAG+AGG | + | chr1.4:5911755-5911774 | None:intergenic | 30.0% | |
| ATGGAACTTTCTATGAGTTT+AGG | - | chr1.4:5911230-5911249 | MS.gene57222:intron | 30.0% | |
| CAAGGATTAAGTAATGTCTA+CGG | + | chr1.4:5913017-5913036 | None:intergenic | 30.0% | |
| CAGTCAATATAGTCAATGTT+TGG | - | chr1.4:5912183-5912202 | MS.gene57222:intron | 30.0% | |
| CTTCATTCATTCAAAAGCAA+TGG | - | chr1.4:5912401-5912420 | MS.gene57222:CDS | 30.0% | |
| CTTGATGAGATAAACTATTC+GGG | - | chr1.4:5911356-5911375 | MS.gene57222:CDS | 30.0% | |
| GATAAAAAACCTTGATACTG+TGG | + | chr1.4:5912605-5912624 | None:intergenic | 30.0% | |
| GCAAATTGTATGATGTTGAA+TGG | - | chr1.4:5912006-5912025 | MS.gene57222:intron | 30.0% | |
| TAGCAAACATATTCTTTCCA+TGG | - | chr1.4:5912324-5912343 | MS.gene57222:CDS | 30.0% | |
| TATCTTGATCCTAATATTGC+CGG | + | chr1.4:5911281-5911300 | None:intergenic | 30.0% | |
| TGAAAAGGAAAGATCAGTTA+TGG | - | chr1.4:5912437-5912456 | MS.gene57222:CDS | 30.0% | |
| TGGAACTTTCTATGAGTTTA+GGG | - | chr1.4:5911231-5911250 | MS.gene57222:intron | 30.0% | |
| TTGATTGAGCGATAAATTCA+AGG | + | chr1.4:5911775-5911794 | None:intergenic | 30.0% | |
| ! | AAGTGAACAATTTTGCATCT+AGG | + | chr1.4:5911910-5911929 | None:intergenic | 30.0% |
| ! | ATATTTATGGTTGGTTGTGT+AGG | - | chr1.4:5913129-5913148 | MS.gene57222:CDS | 30.0% |
| ! | CCTGACTATTAATTTGGAAT+CGG | + | chr1.4:5912889-5912908 | None:intergenic | 30.0% |
| ! | GATAAGGGCTACATTTTTAA+TGG | + | chr1.4:5913063-5913082 | None:intergenic | 30.0% |
| ! | GTCATTAATTTGAGATCGAA+TGG | + | chr1.4:5912954-5912973 | None:intergenic | 30.0% |
| ! | TCATTTGTTACATCACAACA+TGG | - | chr1.4:5910756-5910775 | MS.gene57222:intron | 30.0% |
| ! | TCGAATTAGTGTATGTTTTC+TGG | - | chr1.4:5911615-5911634 | MS.gene57222:CDS | 30.0% |
| !!! | TAGTCTTTATTTTACTCAGG+TGG | - | chr1.4:5912159-5912178 | MS.gene57222:intron | 30.0% |
| !!! | TGATGTTGTTTTGGTTGTTA+AGG | + | chr1.4:5910650-5910669 | None:intergenic | 30.0% |
| AAACATCCAATCACGTAGAA+CGG | + | chr1.4:5912497-5912516 | None:intergenic | 35.0% | |
| AAAGCTCAAGAGAGTAATGT+TGG | - | chr1.4:5910972-5910991 | MS.gene57222:intron | 35.0% | |
| AAAGGAAAGATCAGTTATGG+AGG | - | chr1.4:5912440-5912459 | MS.gene57222:CDS | 35.0% | |
| AAATGCTTCTCTGCATCTTT+AGG | + | chr1.4:5912775-5912794 | None:intergenic | 35.0% | |
| AACGGTTTCTCGACTATATA+AGG | - | chr1.4:5912285-5912304 | MS.gene57222:intron | 35.0% | |
| AAGTTACACACAAGGATCAA+AGG | - | chr1.4:5912076-5912095 | MS.gene57222:intron | 35.0% | |
| AAGTTCCATACTACTACATG+AGG | + | chr1.4:5911219-5911238 | None:intergenic | 35.0% | |
| AATTCGATCAGGACTATGAA+CGG | + | chr1.4:5911602-5911621 | None:intergenic | 35.0% | |
| ACAATGATGTGATGCATTTC+AGG | - | chr1.4:5912746-5912765 | MS.gene57222:CDS | 35.0% | |
| ACATTCCAAAGTTACACACA+AGG | - | chr1.4:5912068-5912087 | MS.gene57222:intron | 35.0% | |
| AGAAAAGCTTGTCGAAATGA+AGG | - | chr1.4:5913229-5913248 | MS.gene57222:CDS | 35.0% | |
| ATTAATAGTCAGGTCAGGAT+CGG | - | chr1.4:5912896-5912915 | MS.gene57222:CDS | 35.0% | |
| ATTTATTCCTATCCTTCGCT+TGG | + | chr1.4:5911429-5911448 | None:intergenic | 35.0% | |
| CCGATTCCAAATTAATAGTC+AGG | - | chr1.4:5912886-5912905 | MS.gene57222:CDS | 35.0% | |
| GACTTAACTCATAGCATGAA+TGG | - | chr1.4:5911170-5911189 | MS.gene57222:intron | 35.0% | |
| GCATACATTTCCTCAATCAA+TGG | + | chr1.4:5913196-5913215 | None:intergenic | 35.0% | |
| GTATTTCCTGAATTACAGCA+AGG | + | chr1.4:5911304-5911323 | None:intergenic | 35.0% | |
| TAGATAATACTTACGGATGG+AGG | + | chr1.4:5912528-5912547 | None:intergenic | 35.0% | |
| TAGCATGAATGGAACAAGAT+CGG | - | chr1.4:5911181-5911200 | MS.gene57222:intron | 35.0% | |
| TATATAAGGACTTGAGAGAG+AGG | - | chr1.4:5912299-5912318 | MS.gene57222:intron | 35.0% | |
| TCCAAATTAATAGTCAGGTC+AGG | - | chr1.4:5912891-5912910 | MS.gene57222:CDS | 35.0% | |
| TCCTGACCTGACTATTAATT+TGG | + | chr1.4:5912895-5912914 | None:intergenic | 35.0% | |
| TTAAGTAATGTCTACGGATC+CGG | + | chr1.4:5913011-5913030 | None:intergenic | 35.0% | |
| TTGATCCTTGTGTGTAACTT+TGG | + | chr1.4:5912076-5912095 | None:intergenic | 35.0% | |
| TTGTTACATCACAACATGGA+AGG | - | chr1.4:5910760-5910779 | MS.gene57222:intron | 35.0% | |
| TTTACATGCAACACACTCTT+AGG | - | chr1.4:5911827-5911846 | MS.gene57222:intron | 35.0% | |
| ! | AAGATACCTTGCTGTAATTC+AGG | - | chr1.4:5911295-5911314 | MS.gene57222:intron | 35.0% |
| ! | GTTGAAAACATGTGGTTTTC+CGG | - | chr1.4:5912989-5913008 | MS.gene57222:CDS | 35.0% |
| ! | TAAACATGTCAAGTTTTCGC+CGG | - | chr1.4:5911259-5911278 | MS.gene57222:intron | 35.0% |
| ! | TCTGGTTTCATTCTTGAACA+TGG | - | chr1.4:5911633-5911652 | MS.gene57222:CDS | 35.0% |
| ! | TTTGATGATTGCCATCCATT+TGG | + | chr1.4:5911038-5911057 | None:intergenic | 35.0% |
| ! | TTTTCAGTTCCTGCTAGAAT+TGG | - | chr1.4:5910828-5910847 | MS.gene57222:intron | 35.0% |
| !! | ATGCTGATTCTTCATTGCAA+AGG | - | chr1.4:5911477-5911496 | MS.gene57222:CDS | 35.0% |
| !! | GGATCGGTTAAAACTGATTT+TGG | - | chr1.4:5912912-5912931 | MS.gene57222:CDS | 35.0% |
| !!! | AGGTTTTTTATCCCCAATTC+CGG | - | chr1.4:5912613-5912632 | MS.gene57222:CDS | 35.0% |
| !!! | TAATCCTTGCTTGTTTTGAG+AGG | - | chr1.4:5913028-5913047 | MS.gene57222:CDS | 35.0% |
| ACAACATGGAAGGACTATTG+TGG | - | chr1.4:5910770-5910789 | MS.gene57222:intron | 40.0% | |
| ACAAGCCTCATGTAGTAGTA+TGG | - | chr1.4:5911211-5911230 | MS.gene57222:intron | 40.0% | |
| ACTCTTGATGCTTCTACAGA+TGG | - | chr1.4:5911526-5911545 | MS.gene57222:CDS | 40.0% | |
| AGAACGGAAACAGATCTTTC+TGG | + | chr1.4:5912481-5912500 | None:intergenic | 40.0% | |
| AGATCATGGATGAATGCATG+TGG | - | chr1.4:5912718-5912737 | MS.gene57222:CDS | 40.0% | |
| AGCATGAATGGAACAAGATC+GGG | - | chr1.4:5911182-5911201 | MS.gene57222:intron | 40.0% | |
| AGTTCCTCTCAAAACAAGCA+AGG | + | chr1.4:5913035-5913054 | None:intergenic | 40.0% | |
| ATAGGGAACATCGATCCAAA+TGG | - | chr1.4:5911020-5911039 | MS.gene57222:intron | 40.0% | |
| ATCACCAAAAGTTGCACACA+AGG | + | chr1.4:5910925-5910944 | None:intergenic | 40.0% | |
| GCAAAGTGTGCATGTATTTG+TGG | + | chr1.4:5912259-5912278 | None:intergenic | 40.0% | |
| GGAATTCCAATACTTTCTGG+TGG | - | chr1.4:5910675-5910694 | MS.gene57222:CDS | 40.0% | |
| GGTTTATAAATGCGCGTGTT+CGG | - | chr1.4:5913161-5913180 | MS.gene57222:CDS | 40.0% | |
| GTACACTGCTCAAATGAACA+TGG | - | chr1.4:5910548-5910567 | MS.gene57222:CDS | 40.0% | |
| TAACAGTTCATGGTCAGAAG+AGG | - | chr1.4:5912357-5912376 | MS.gene57222:CDS | 40.0% | |
| TCACCAAAAGTTGCACACAA+GGG | + | chr1.4:5910924-5910943 | None:intergenic | 40.0% | |
| TCAGCATAAGCTTCTGACAT+AGG | + | chr1.4:5911464-5911483 | None:intergenic | 40.0% | |
| TCATGTCATATTCGCCGATA+GGG | - | chr1.4:5911003-5911022 | MS.gene57222:intron | 40.0% | |
| TCTTGAAGACCAATTCTAGC+AGG | + | chr1.4:5910840-5910859 | None:intergenic | 40.0% | |
| TGGTTGTGTAGGTATCAAAC+TGG | - | chr1.4:5913140-5913159 | MS.gene57222:CDS | 40.0% | |
| TTCATGGTCAGAAGAGGATA+AGG | - | chr1.4:5912363-5912382 | MS.gene57222:CDS | 40.0% | |
| TTCATGTCATATTCGCCGAT+AGG | - | chr1.4:5911002-5911021 | MS.gene57222:intron | 40.0% | |
| TTTGGATCGATGTTCCCTAT+CGG | + | chr1.4:5911020-5911039 | None:intergenic | 40.0% | |
| ! | AAGTTTTCGCCGGCAATATT+AGG | - | chr1.4:5911269-5911288 | MS.gene57222:intron | 40.0% |
| ! | GAGATAAACTATTCGGGTTC+TGG | - | chr1.4:5911362-5911381 | MS.gene57222:CDS | 40.0% |
| ! | GCTATGGAAACCATTGATTG+AGG | - | chr1.4:5913183-5913202 | MS.gene57222:CDS | 40.0% |
| ! | TGAATGAGTGGCTTTTGTGT+TGG | + | chr1.4:5910612-5910631 | None:intergenic | 40.0% |
| ! | TGCACACTTTGCTGTCAAAA+CGG | - | chr1.4:5912267-5912286 | MS.gene57222:intron | 40.0% |
| ! | TGTTCTCCACCAGAAAGTAT+TGG | + | chr1.4:5910684-5910703 | None:intergenic | 40.0% |
| AACACAACCAAGCGAAGGAT+AGG | - | chr1.4:5911419-5911438 | MS.gene57222:CDS | 45.0% | |
| ACTGCTCAAATGAACATGGC+TGG | - | chr1.4:5910552-5910571 | MS.gene57222:CDS | 45.0% | |
| ATGAGAGCAGTGAAAGAAGC+AGG | - | chr1.4:5913257-5913276 | MS.gene57222:CDS | 45.0% | |
| CACCAAAAGTTGCACACAAG+GGG | + | chr1.4:5910923-5910942 | None:intergenic | 45.0% | |
| CTGTTTCCGTTCTACGTGAT+TGG | - | chr1.4:5912488-5912507 | MS.gene57222:CDS | 45.0% | |
| CTTGCAACATCACCAACTTC+AGG | - | chr1.4:5911122-5911141 | MS.gene57222:intron | 45.0% | |
| GAATGGAACAAGATCGGGTT+TGG | - | chr1.4:5911187-5911206 | MS.gene57222:intron | 45.0% | |
| GGAACATCGATCCAAATGGA+TGG | - | chr1.4:5911024-5911043 | MS.gene57222:CDS | 45.0% | |
| GGGCTCTTCAACAGTTGAAA+AGG | - | chr1.4:5912422-5912441 | MS.gene57222:CDS | 45.0% | |
| GGTCAATGCTCAGAGATTAG+TGG | - | chr1.4:5911143-5911162 | MS.gene57222:intron | 45.0% | |
| ! | AACCCCTTGTGTGCAACTTT+TGG | - | chr1.4:5910918-5910937 | MS.gene57222:intron | 45.0% |
| ! | TATTCGGGTTCTGGTGTTAG+AGG | - | chr1.4:5911371-5911390 | MS.gene57222:CDS | 45.0% |
| !! | AAGTGGGTTGACTAGAAGTC+AGG | - | chr1.4:5912807-5912826 | MS.gene57222:CDS | 45.0% |
| !! | TCTGAGCATTGACCTGAAGT+TGG | + | chr1.4:5911137-5911156 | None:intergenic | 45.0% |
| ATCAGTTATGGAGGCCCCAA+AGG | - | chr1.4:5912449-5912468 | MS.gene57222:CDS | 50.0% | |
| CGATTGCAGCCACAGTATCA+AGG | - | chr1.4:5912593-5912612 | MS.gene57222:CDS | 50.0% | |
| TAAATGCGCGTGTTCGGCTA+TGG | - | chr1.4:5913167-5913186 | MS.gene57222:CDS | 50.0% | |
| TCAGTTATGGAGGCCCCAAA+GGG | - | chr1.4:5912450-5912469 | MS.gene57222:CDS | 50.0% | |
| TGTCGAACACAACCAAGCGA+AGG | - | chr1.4:5911414-5911433 | MS.gene57222:CDS | 50.0% | |
| ! | ATCTTTCTGGCAAGCCCCTT+TGG | + | chr1.4:5912468-5912487 | None:intergenic | 50.0% |
| ! | TCTTTCTGGCAAGCCCCTTT+GGG | + | chr1.4:5912467-5912486 | None:intergenic | 50.0% |
| AGCTGATGCAGCCGGAATTG+GGG | + | chr1.4:5912627-5912646 | None:intergenic | 55.0% | |
| CAGCTGATGCAGCCGGAATT+GGG | + | chr1.4:5912628-5912647 | None:intergenic | 55.0% | |
| CAGTTATGGAGGCCCCAAAG+GGG | - | chr1.4:5912451-5912470 | MS.gene57222:CDS | 55.0% | |
| CGAAATGCAGCTGATGCAGC+CGG | + | chr1.4:5912635-5912654 | None:intergenic | 55.0% | |
| ! | CTTTCTGGCAAGCCCCTTTG+GGG | + | chr1.4:5912466-5912485 | None:intergenic | 55.0% |
| GCAGCTGATGCAGCCGGAAT+TGG | + | chr1.4:5912629-5912648 | None:intergenic | 60.0% | |
| ! | GGTTCTGGTGTTAGAGGCCG+AGG | - | chr1.4:5911377-5911396 | MS.gene57222:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 5910534 | 5913315 | 5910534 | ID=MS.gene57222 |
| chr1.4 | mRNA | 5910534 | 5913315 | 5910534 | ID=MS.gene57222.t1;Parent=MS.gene57222 |
| chr1.4 | exon | 5912302 | 5913315 | 5912302 | ID=MS.gene57222.t1.exon1;Parent=MS.gene57222.t1 |
| chr1.4 | CDS | 5912302 | 5913315 | 5912302 | ID=cds.MS.gene57222.t1;Parent=MS.gene57222.t1 |
| chr1.4 | exon | 5911316 | 5911671 | 5911316 | ID=MS.gene57222.t1.exon2;Parent=MS.gene57222.t1 |
| chr1.4 | CDS | 5911316 | 5911671 | 5911316 | ID=cds.MS.gene57222.t1;Parent=MS.gene57222.t1 |
| chr1.4 | exon | 5911021 | 5911081 | 5911021 | ID=MS.gene57222.t1.exon3;Parent=MS.gene57222.t1 |
| chr1.4 | CDS | 5911021 | 5911081 | 5911021 | ID=cds.MS.gene57222.t1;Parent=MS.gene57222.t1 |
| chr1.4 | exon | 5910534 | 5910698 | 5910534 | ID=MS.gene57222.t1.exon4;Parent=MS.gene57222.t1 |
| chr1.4 | CDS | 5910534 | 5910698 | 5910534 | ID=cds.MS.gene57222.t1;Parent=MS.gene57222.t1 |
| Gene Sequence |
| Protein sequence |
