Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59513.t1 | XP_003592995.2 | 100 | 127 | 0 | 0 | 1 | 127 | 424 | 550 | 1.90E-64 | 255.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59513.t1 | A0A072V445 | 100.0 | 127 | 0 | 0 | 1 | 127 | 424 | 550 | 1.4e-64 | 255.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene59513 | 0.815091 | 1.10E-51 | -1.69E-46 |
| MS.gene050306 | MS.gene59513 | 0.805863 | 1.09E-49 | -1.69E-46 |
| MS.gene050361 | MS.gene59513 | 0.810833 | 9.47E-51 | -1.69E-46 |
| MS.gene050384 | MS.gene59513 | 0.802537 | 5.35E-49 | -1.69E-46 |
| MS.gene050646 | MS.gene59513 | 0.811453 | 6.95E-51 | -1.69E-46 |
| MS.gene051066 | MS.gene59513 | 0.817019 | 4.10E-52 | -1.69E-46 |
| MS.gene051198 | MS.gene59513 | 0.823616 | 1.26E-53 | -1.69E-46 |
| MS.gene051800 | MS.gene59513 | 0.807427 | 5.08E-50 | -1.69E-46 |
| MS.gene052674 | MS.gene59513 | 0.800713 | 1.27E-48 | -1.69E-46 |
| MS.gene052675 | MS.gene59513 | 0.80648 | 8.06E-50 | -1.69E-46 |
| MS.gene052677 | MS.gene59513 | 0.808889 | 2.48E-50 | -1.69E-46 |
| MS.gene053931 | MS.gene59513 | 0.806933 | 6.47E-50 | -1.69E-46 |
| MS.gene05510 | MS.gene59513 | 0.808485 | 3.03E-50 | -1.69E-46 |
| MS.gene056480 | MS.gene59513 | 0.802164 | 6.39E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene59513 | 0.811626 | 6.37E-51 | -1.69E-46 |
| MS.gene057869 | MS.gene59513 | 0.841647 | 4.18E-58 | -1.69E-46 |
| MS.gene058010 | MS.gene59513 | 0.841963 | 3.45E-58 | -1.69E-46 |
| MS.gene058595 | MS.gene59513 | 0.809597 | 1.75E-50 | -1.69E-46 |
| MS.gene059161 | MS.gene59513 | 0.812271 | 4.61E-51 | -1.69E-46 |
| MS.gene060011 | MS.gene59513 | 0.811109 | 8.25E-51 | -1.69E-46 |
| MS.gene060310 | MS.gene59513 | 0.815593 | 8.54E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59513.t1 | MTR_2g006520 | 100.000 | 127 | 0 | 0 | 1 | 127 | 424 | 550 | 4.69e-86 | 271 |
| MS.gene59513.t1 | MTR_2g006520 | 100.000 | 127 | 0 | 0 | 1 | 127 | 424 | 550 | 4.92e-85 | 271 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59513.t1 | AT1G22930 | 64.394 | 132 | 40 | 2 | 1 | 130 | 418 | 544 | 1.01e-49 | 171 |
| MS.gene59513.t1 | AT1G22930 | 64.394 | 132 | 40 | 2 | 1 | 130 | 307 | 433 | 1.06e-49 | 171 |
| MS.gene59513.t1 | AT4G09150 | 56.250 | 96 | 40 | 2 | 1 | 95 | 414 | 508 | 6.35e-26 | 103 |
| MS.gene59513.t1 | AT4G09150 | 56.250 | 96 | 40 | 2 | 1 | 95 | 414 | 508 | 6.41e-26 | 103 |
Find 26 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTTGTGCTTATATGATAT+TGG | 0.264256 | 8.1:-74840035 | None:intergenic |
| GGTTAAGGATGCTCGATCTT+TGG | 0.289741 | 8.1:-74839777 | MS.gene59513:CDS |
| TGACTAATACCAACTCCTTC+TGG | 0.322189 | 8.1:+74839689 | None:intergenic |
| CTTTGTGCTTATATGATATT+GGG | 0.325531 | 8.1:-74840034 | None:intergenic |
| TCGATCTTTGGAGGATGATT+TGG | 0.346054 | 8.1:-74839765 | MS.gene59513:CDS |
| AATTATAGAACTTGAGTGTA+TGG | 0.401215 | 8.1:-74838728 | MS.gene59513:CDS |
| CAACTTGTTACTTCCCTTGA+AGG | 0.405951 | 8.1:-74838814 | MS.gene59513:CDS |
| TTCCCTTGAAGGTAGTTTGA+AGG | 0.419566 | 8.1:-74838803 | MS.gene59513:CDS |
| AACAAATTCTTGAGCAGACT+TGG | 0.423461 | 8.1:+74839961 | None:intergenic |
| AAACCTTCAAACTACCTTCA+AGG | 0.438903 | 8.1:+74838800 | None:intergenic |
| ATTGTACTGAAAACTGCATC+TGG | 0.442866 | 8.1:+74840007 | None:intergenic |
| GGGCGAGCGTGAAATTGCTT+TGG | 0.447463 | 8.1:-74839984 | MS.gene59513:CDS |
| TATGAAAGCTATTCAGCATC+AGG | 0.470987 | 8.1:-74839663 | MS.gene59513:intron |
| TAGAGCAGCTTGCCAGCTTG+AGG | 0.496691 | 8.1:-74839741 | MS.gene59513:CDS |
| TAAGGATGCTCGATCTTTGG+AGG | 0.517564 | 8.1:-74839774 | MS.gene59513:CDS |
| CAAGGGAAGTAACAAGTTGA+AGG | 0.519878 | 8.1:+74838818 | None:intergenic |
| AACCTTCAAACTACCTTCAA+GGG | 0.529898 | 8.1:+74838801 | None:intergenic |
| AGCAGCAAGCTGTGATCTGA+AGG | 0.542055 | 8.1:+74839847 | None:intergenic |
| AAGTTAACTCCAGAAGGAGT+TGG | 0.569801 | 8.1:-74839698 | MS.gene59513:CDS |
| ACTTGCAAGTTAACTCCAGA+AGG | 0.572489 | 8.1:-74839704 | MS.gene59513:CDS |
| GAATCATAGAAGCCTCAAGC+TGG | 0.572618 | 8.1:+74839729 | None:intergenic |
| ATTATAGAACTTGAGTGTAT+GGG | 0.576982 | 8.1:-74838727 | MS.gene59513:CDS |
| GCTTCTGACGTAGAAGAAGT+AGG | 0.581602 | 8.1:-74838751 | MS.gene59513:CDS |
| CTGATAAAGATTATACAAGA+GGG | 0.588879 | 8.1:-74839923 | MS.gene59513:CDS |
| GCTGATAAAGATTATACAAG+AGG | 0.589849 | 8.1:-74839924 | MS.gene59513:CDS |
| TGATAAAGATTATACAAGAG+GGG | 0.699828 | 8.1:-74839922 | MS.gene59513:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTTTTTATTGTGATAAT+TGG | + | chr8.1:74839573-74839592 | None:intergenic | 10.0% |
| ! | AAAGGAAAAATAACATCCAA+GGG | - | chr8.1:74839477-74839496 | MS.gene59513:intron | 25.0% |
| ! | AATTATAGAACTTGAGTGTA+TGG | - | chr8.1:74840008-74840027 | MS.gene59513:CDS | 25.0% |
| ! | ACTGTACATTTCATAGTAAT+GGG | - | chr8.1:74839310-74839329 | MS.gene59513:intron | 25.0% |
| ! | ATCAATAACTACCATTGAAA+AGG | + | chr8.1:74839129-74839148 | None:intergenic | 25.0% |
| ! | ATTATAGAACTTGAGTGTAT+GGG | - | chr8.1:74840009-74840028 | MS.gene59513:CDS | 25.0% |
| ! | CTGATAAAGATTATACAAGA+GGG | - | chr8.1:74838813-74838832 | MS.gene59513:CDS | 25.0% |
| ! | GTACAGTAAAATTTCAAATG+CGG | + | chr8.1:74839297-74839316 | None:intergenic | 25.0% |
| ! | TAAAGGAAAAATAACATCCA+AGG | - | chr8.1:74839476-74839495 | MS.gene59513:intron | 25.0% |
| ! | TACTGTACATTTCATAGTAA+TGG | - | chr8.1:74839309-74839328 | MS.gene59513:intron | 25.0% |
| ! | TGATAAAGATTATACAAGAG+GGG | - | chr8.1:74838814-74838833 | MS.gene59513:CDS | 25.0% |
| ! | TTATCCGATTTCAAAATTCA+TGG | + | chr8.1:74839721-74839740 | None:intergenic | 25.0% |
| !! | TACGAAAATGGTGAAATTTT+TGG | + | chr8.1:74839888-74839907 | None:intergenic | 25.0% |
| !! | TCTTCATCAGAACTTTTTAT+TGG | + | chr8.1:74838840-74838859 | None:intergenic | 25.0% |
| !!! | GAATTTTGAAATCGGATAAA+GGG | - | chr8.1:74839722-74839741 | MS.gene59513:CDS | 25.0% |
| !!! | GATAATTGGCATAATTTGTT+AGG | + | chr8.1:74839559-74839578 | None:intergenic | 25.0% |
| !!! | TGAATTTTGAAATCGGATAA+AGG | - | chr8.1:74839721-74839740 | MS.gene59513:CDS | 25.0% |
| AGAATCCAAGTACAAAGTAA+GGG | - | chr8.1:74839773-74839792 | MS.gene59513:CDS | 30.0% | |
| ATATATGCAAATGCGGAAAT+TGG | + | chr8.1:74839346-74839365 | None:intergenic | 30.0% | |
| GCGAAAAATATATGCAAATG+CGG | + | chr8.1:74839353-74839372 | None:intergenic | 30.0% | |
| GCTGATAAAGATTATACAAG+AGG | - | chr8.1:74838812-74838831 | MS.gene59513:CDS | 30.0% | |
| TAGAATCCAAGTACAAAGTA+AGG | - | chr8.1:74839772-74839791 | MS.gene59513:CDS | 30.0% | |
| TTTGTTAGGAAAATGCTTCA+TGG | + | chr8.1:74839545-74839564 | None:intergenic | 30.0% | |
| !! | ATTTGAATTGTTTTGTGGTC+TGG | - | chr8.1:74838934-74838953 | MS.gene59513:intron | 30.0% |
| !! | CTCGTATTTGAATTGTTTTG+TGG | - | chr8.1:74838929-74838948 | MS.gene59513:intron | 30.0% |
| !!! | CTTGCCATGAATTTTGAAAT+CGG | - | chr8.1:74839714-74839733 | MS.gene59513:CDS | 30.0% |
| AAAACTCACAGCTATTGACA+TGG | + | chr8.1:74839106-74839125 | None:intergenic | 35.0% | |
| AAACCTTCAAACTACCTTCA+AGG | + | chr8.1:74839939-74839958 | None:intergenic | 35.0% | |
| AACAAATTCTTGAGCAGACT+TGG | + | chr8.1:74838778-74838797 | None:intergenic | 35.0% | |
| AACCTTCAAACTACCTTCAA+GGG | + | chr8.1:74839938-74839957 | None:intergenic | 35.0% | |
| ATTGTACTGAAAACTGCATC+TGG | + | chr8.1:74838732-74838751 | None:intergenic | 35.0% | |
| GAGAGAATTCAGTTGGTTAA+AGG | - | chr8.1:74839459-74839478 | MS.gene59513:intron | 35.0% | |
| TATGAAAGCTATTCAGCATC+AGG | - | chr8.1:74839073-74839092 | MS.gene59513:intron | 35.0% | |
| TCTATCCACAATTTCGCTTT+CGG | + | chr8.1:74839756-74839775 | None:intergenic | 35.0% | |
| ! | AGATGCAGTTTTCAGTACAA+TGG | - | chr8.1:74838731-74838750 | MS.gene59513:CDS | 35.0% |
| ! | CTGTGAGTTTTCCTTTTCAA+TGG | - | chr8.1:74839115-74839134 | MS.gene59513:intron | 35.0% |
| ! | GATGCAGTTTTCAGTACAAT+GGG | - | chr8.1:74838732-74838751 | MS.gene59513:CDS | 35.0% |
| ! | TTATTTGTTAGTGCAGACTG+AGG | + | chr8.1:74839231-74839250 | None:intergenic | 35.0% |
| ! | TTTGCATATATTTTTCGCCC+AGG | - | chr8.1:74839355-74839374 | MS.gene59513:intron | 35.0% |
| !!! | TTGCTGCTTTTGATAAAGCA+TGG | - | chr8.1:74838904-74838923 | MS.gene59513:intron | 35.0% |
| !!! | TTTTGAAATCGGATAAAGGG+CGG | - | chr8.1:74839725-74839744 | MS.gene59513:CDS | 35.0% |
| AAGAACCGAAAGCGAAATTG+TGG | - | chr8.1:74839748-74839767 | MS.gene59513:CDS | 40.0% | |
| AAGTTAACTCCAGAAGGAGT+TGG | - | chr8.1:74839038-74839057 | MS.gene59513:intron | 40.0% | |
| AATTTCCGCAGTTCTCTTGA+CGG | - | chr8.1:74839249-74839268 | MS.gene59513:intron | 40.0% | |
| ACTTGCAAGTTAACTCCAGA+AGG | - | chr8.1:74839032-74839051 | MS.gene59513:intron | 40.0% | |
| AGCAACCCTTACTTTGTACT+TGG | + | chr8.1:74839781-74839800 | None:intergenic | 40.0% | |
| GGAAAAATAACATCCAAGGG+TGG | - | chr8.1:74839480-74839499 | MS.gene59513:intron | 40.0% | |
| TGACTAATACCAACTCCTTC+TGG | + | chr8.1:74839050-74839069 | None:intergenic | 40.0% | |
| TTCCCTTGAAGGTAGTTTGA+AGG | - | chr8.1:74839933-74839952 | MS.gene59513:CDS | 40.0% | |
| ! | CAAGGGAAGTAACAAGTTGA+AGG | + | chr8.1:74839921-74839940 | None:intergenic | 40.0% |
| ! | CATGAATAGCGGAAATCATG+CGG | - | chr8.1:74839414-74839433 | MS.gene59513:intron | 40.0% |
| ! | TCGATCTTTGGAGGATGATT+TGG | - | chr8.1:74838971-74838990 | MS.gene59513:intron | 40.0% |
| ! | TTTTGTGGTCTGGAAGGTTA+AGG | - | chr8.1:74838944-74838963 | MS.gene59513:intron | 40.0% |
| !! | CAACTTGTTACTTCCCTTGA+AGG | - | chr8.1:74839922-74839941 | MS.gene59513:CDS | 40.0% |
| !! | GAATTGTTTTGTGGTCTGGA+AGG | - | chr8.1:74838938-74838957 | MS.gene59513:intron | 40.0% |
| AAAATCCCACGAATTCCGAG+TGG | + | chr8.1:74839808-74839827 | None:intergenic | 45.0% | |
| AAATCCCACGAATTCCGAGT+GGG | + | chr8.1:74839807-74839826 | None:intergenic | 45.0% | |
| AACCATGCTGATCGCGAATA+CGG | + | chr8.1:74839836-74839855 | None:intergenic | 45.0% | |
| AGATAGACCGCTGGAATTTC+TGG | - | chr8.1:74839857-74839876 | MS.gene59513:CDS | 45.0% | |
| ATGCGGTGGAAAGCAAAACA+TGG | - | chr8.1:74839431-74839450 | MS.gene59513:intron | 45.0% | |
| ATGCTCTTCTCTTGTGAGCA+TGG | - | chr8.1:74839200-74839219 | MS.gene59513:intron | 45.0% | |
| CCAGGAATTTCATAGAGCCT+GGG | + | chr8.1:74839375-74839394 | None:intergenic | 45.0% | |
| CGCATGATTTCCGCTATTCA+TGG | + | chr8.1:74839416-74839435 | None:intergenic | 45.0% | |
| GAATCATAGAAGCCTCAAGC+TGG | + | chr8.1:74839010-74839029 | None:intergenic | 45.0% | |
| GACGAATTAATTGCCACCCT+TGG | + | chr8.1:74839496-74839515 | None:intergenic | 45.0% | |
| GCTTCTGACGTAGAAGAAGT+AGG | - | chr8.1:74839985-74840004 | MS.gene59513:CDS | 45.0% | |
| GGTGGAAAGCAAAACATGGA+GGG | - | chr8.1:74839435-74839454 | MS.gene59513:intron | 45.0% | |
| GGTTAAGGATGCTCGATCTT+TGG | - | chr8.1:74838959-74838978 | MS.gene59513:intron | 45.0% | |
| GGTTTCTCCAGAAATTCCAG+CGG | + | chr8.1:74839867-74839886 | None:intergenic | 45.0% | |
| TAAGGATGCTCGATCTTTGG+AGG | - | chr8.1:74838962-74838981 | MS.gene59513:intron | 45.0% | |
| TGCAAATGCGGAAATTGGTG+CGG | + | chr8.1:74839341-74839360 | None:intergenic | 45.0% | |
| TGTGATCTTGCGTGAACCTT+TGG | - | chr8.1:74839623-74839642 | MS.gene59513:intron | 45.0% | |
| ! | GAGCATGAAGTCAGCTTTTG+AGG | + | chr8.1:74839186-74839205 | None:intergenic | 45.0% |
| ! | GGTGCTGTGAGATACGAAAA+TGG | + | chr8.1:74839900-74839919 | None:intergenic | 45.0% |
| ACGTACCGTCAAGAGAACTG+CGG | + | chr8.1:74839257-74839276 | None:intergenic | 50.0% | |
| AGCAGCAAGCTGTGATCTGA+AGG | + | chr8.1:74838892-74838911 | None:intergenic | 50.0% | |
| CAACTTCGTGCCATCACCAA+AGG | + | chr8.1:74839642-74839661 | None:intergenic | 50.0% | |
| CCCAGGCTCTATGAAATTCC+TGG | - | chr8.1:74839372-74839391 | MS.gene59513:intron | 50.0% | |
| CGGTGGAAAGCAAAACATGG+AGG | - | chr8.1:74839434-74839453 | MS.gene59513:intron | 50.0% | |
| CTATTCATGGTCGTGTGCAG+TGG | + | chr8.1:74839403-74839422 | None:intergenic | 50.0% | |
| CTGCACACGACCATGAATAG+CGG | - | chr8.1:74839403-74839422 | MS.gene59513:intron | 50.0% | |
| CTTGCGTGAACCTTTGGTGA+TGG | - | chr8.1:74839629-74839648 | MS.gene59513:intron | 50.0% | |
| GCCAGGAATTTCATAGAGCC+TGG | + | chr8.1:74839376-74839395 | None:intergenic | 50.0% | |
| GGAGGGAGAGAGAATTCAGT+TGG | - | chr8.1:74839452-74839471 | MS.gene59513:intron | 50.0% | |
| TTCCGTATTCGCGATCAGCA+TGG | - | chr8.1:74839831-74839850 | MS.gene59513:CDS | 50.0% | |
| ! | GAATAGCGGAAATCATGCGG+TGG | - | chr8.1:74839417-74839436 | MS.gene59513:intron | 50.0% |
| GCATGGTTGAGATAGACCGC+TGG | - | chr8.1:74839848-74839867 | MS.gene59513:CDS | 55.0% | |
| GGGCGAGCGTGAAATTGCTT+TGG | - | chr8.1:74838752-74838771 | MS.gene59513:CDS | 55.0% | |
| TAAGGGTTGCTCCTCCCACT+CGG | - | chr8.1:74839790-74839809 | MS.gene59513:CDS | 55.0% | |
| TAGAGCAGCTTGCCAGCTTG+AGG | - | chr8.1:74838995-74839014 | MS.gene59513:intron | 55.0% | |
| TCCTCCCACTCGGAATTCGT+GGG | - | chr8.1:74839800-74839819 | MS.gene59513:CDS | 55.0% | |
| TTCATGGTCGTGTGCAGTGG+CGG | + | chr8.1:74839400-74839419 | None:intergenic | 55.0% | |
| CTCCTCCCACTCGGAATTCG+TGG | - | chr8.1:74839799-74839818 | MS.gene59513:CDS | 60.0% | |
| TCCCACGAATTCCGAGTGGG+AGG | + | chr8.1:74839804-74839823 | None:intergenic | 60.0% | |
| ! | TCGTGTGCAGTGGCGGAGCC+AGG | + | chr8.1:74839393-74839412 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 74838714 | 74840044 | 74838714 | ID=MS.gene59513 |
| chr8.1 | mRNA | 74838714 | 74840044 | 74838714 | ID=MS.gene59513.t1;Parent=MS.gene59513 |
| chr8.1 | exon | 74839664 | 74840044 | 74839664 | ID=MS.gene59513.t1.exon1;Parent=MS.gene59513.t1 |
| chr8.1 | CDS | 74839664 | 74840044 | 74839664 | ID=cds.MS.gene59513.t1;Parent=MS.gene59513.t1 |
| chr8.1 | exon | 74838714 | 74838842 | 74838714 | ID=MS.gene59513.t1.exon2;Parent=MS.gene59513.t1 |
| chr8.1 | CDS | 74838714 | 74838842 | 74838714 | ID=cds.MS.gene59513.t1;Parent=MS.gene59513.t1 |
| Gene Sequence |
| Protein sequence |
