Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59957.t1 | XP_003609366.1 | 96.9 | 224 | 7 | 0 | 1 | 224 | 1 | 224 | 4.50E-74 | 287.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59957.t1 | O82081 | 43.2 | 125 | 67 | 2 | 9 | 133 | 6 | 126 | 3.5e-22 | 106.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59957.t1 | G7JE55 | 96.9 | 224 | 7 | 0 | 1 | 224 | 1 | 224 | 3.3e-74 | 287.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049281 | MS.gene59957 | 0.841268 | 5.27E-58 | -1.69E-46 |
| MS.gene050363 | MS.gene59957 | 0.876796 | 1.05E-68 | -1.69E-46 |
| MS.gene050381 | MS.gene59957 | 0.831146 | 1.97E-55 | -1.69E-46 |
| MS.gene050883 | MS.gene59957 | 0.816817 | 4.55E-52 | -1.69E-46 |
| MS.gene051127 | MS.gene59957 | 0.819557 | 1.09E-52 | -1.69E-46 |
| MS.gene051946 | MS.gene59957 | 0.811048 | 8.51E-51 | -1.69E-46 |
| MS.gene053549 | MS.gene59957 | 0.814441 | 1.54E-51 | -1.69E-46 |
| MS.gene054959 | MS.gene59957 | 0.818998 | 1.46E-52 | -1.69E-46 |
| MS.gene055551 | MS.gene59957 | 0.813327 | 2.71E-51 | -1.69E-46 |
| MS.gene056310 | MS.gene59957 | 0.834568 | 2.78E-56 | -1.69E-46 |
| MS.gene056311 | MS.gene59957 | 0.822393 | 2.43E-53 | -1.69E-46 |
| MS.gene056313 | MS.gene59957 | 0.821379 | 4.17E-53 | -1.69E-46 |
| MS.gene056408 | MS.gene59957 | 0.855707 | 5.27E-62 | -1.69E-46 |
| MS.gene057293 | MS.gene59957 | 0.809831 | 1.56E-50 | -1.69E-46 |
| MS.gene058628 | MS.gene59957 | 0.800612 | 1.33E-48 | -1.69E-46 |
| MS.gene058629 | MS.gene59957 | 0.803171 | 3.96E-49 | -1.69E-46 |
| MS.gene058630 | MS.gene59957 | 0.815631 | 8.37E-52 | -1.69E-46 |
| MS.gene059694 | MS.gene59957 | 0.809297 | 2.03E-50 | -1.69E-46 |
| MS.gene059697 | MS.gene59957 | 0.816343 | 5.81E-52 | -1.69E-46 |
| MS.gene060808 | MS.gene59957 | 0.916231 | 2.28E-85 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59957.t1 | MTR_4g114870 | 96.875 | 224 | 7 | 0 | 1 | 224 | 1 | 224 | 2.12e-152 | 428 |
| MS.gene59957.t1 | MTR_4g114870 | 57.692 | 104 | 44 | 0 | 25 | 128 | 187 | 290 | 3.05e-36 | 131 |
| MS.gene59957.t1 | MTR_6g083240 | 57.225 | 173 | 60 | 4 | 1 | 171 | 1 | 161 | 3.71e-55 | 174 |
| MS.gene59957.t1 | MTR_5g006040 | 43.885 | 139 | 70 | 4 | 29 | 166 | 26 | 157 | 6.97e-33 | 117 |
| MS.gene59957.t1 | MTR_0334s0010 | 46.875 | 128 | 65 | 3 | 1 | 128 | 1 | 125 | 7.43e-33 | 118 |
| MS.gene59957.t1 | MTR_4g067200 | 42.308 | 130 | 72 | 3 | 1 | 128 | 1 | 129 | 4.95e-32 | 115 |
| MS.gene59957.t1 | MTR_4g066110 | 42.308 | 130 | 72 | 3 | 1 | 128 | 1 | 129 | 4.95e-32 | 115 |
| MS.gene59957.t1 | MTR_3g099570 | 44.444 | 117 | 60 | 2 | 27 | 143 | 24 | 135 | 5.56e-28 | 105 |
| MS.gene59957.t1 | MTR_3g099580 | 49.020 | 102 | 47 | 2 | 27 | 128 | 24 | 120 | 2.84e-27 | 103 |
| MS.gene59957.t1 | MTR_1g104800 | 41.096 | 146 | 76 | 3 | 31 | 176 | 27 | 162 | 8.21e-27 | 102 |
| MS.gene59957.t1 | MTR_3g099980 | 48.544 | 103 | 48 | 2 | 27 | 128 | 24 | 122 | 3.65e-26 | 101 |
| MS.gene59957.t1 | MTR_3g099680 | 48.544 | 103 | 48 | 2 | 27 | 128 | 24 | 122 | 4.21e-26 | 101 |
| MS.gene59957.t1 | MTR_1g090420 | 34.659 | 176 | 106 | 4 | 11 | 181 | 10 | 181 | 7.52e-26 | 100 |
| MS.gene59957.t1 | MTR_8g099220 | 36.434 | 129 | 79 | 2 | 1 | 128 | 1 | 127 | 1.66e-25 | 97.1 |
| MS.gene59957.t1 | MTR_8g007020 | 40.698 | 172 | 84 | 7 | 1 | 170 | 1 | 156 | 9.75e-24 | 94.0 |
| MS.gene59957.t1 | MTR_2g088990 | 37.681 | 138 | 79 | 4 | 27 | 163 | 23 | 154 | 2.65e-22 | 91.3 |
| MS.gene59957.t1 | MTR_4g114830 | 40.984 | 122 | 69 | 3 | 25 | 144 | 25 | 145 | 7.17e-22 | 88.6 |
| MS.gene59957.t1 | MTR_2g043730 | 40.000 | 120 | 61 | 5 | 11 | 127 | 12 | 123 | 2.50e-21 | 85.9 |
| MS.gene59957.t1 | MTR_2g101300 | 34.667 | 150 | 94 | 3 | 12 | 160 | 10 | 156 | 3.22e-21 | 87.4 |
| MS.gene59957.t1 | MTR_7g086140 | 37.795 | 127 | 64 | 4 | 9 | 129 | 6 | 123 | 1.03e-20 | 85.5 |
| MS.gene59957.t1 | MTR_7g086190 | 37.795 | 127 | 64 | 4 | 9 | 129 | 6 | 123 | 1.03e-20 | 85.5 |
| MS.gene59957.t1 | MTR_1g105130 | 37.190 | 121 | 71 | 2 | 11 | 130 | 7 | 123 | 1.43e-20 | 84.7 |
| MS.gene59957.t1 | MTR_1g105120 | 37.190 | 121 | 71 | 2 | 11 | 130 | 7 | 123 | 1.43e-20 | 84.7 |
| MS.gene59957.t1 | MTR_7g086090 | 37.500 | 120 | 71 | 1 | 9 | 128 | 6 | 121 | 5.77e-20 | 85.5 |
| MS.gene59957.t1 | MTR_1g014120 | 33.333 | 138 | 89 | 2 | 13 | 147 | 9 | 146 | 6.12e-20 | 84.0 |
| MS.gene59957.t1 | MTR_7g086160 | 37.500 | 128 | 64 | 5 | 9 | 129 | 6 | 124 | 1.16e-19 | 82.8 |
| MS.gene59957.t1 | MTR_8g007035 | 41.935 | 124 | 60 | 5 | 10 | 131 | 10 | 123 | 3.05e-19 | 82.4 |
| MS.gene59957.t1 | MTR_8g088830 | 42.268 | 97 | 50 | 1 | 31 | 127 | 30 | 120 | 4.12e-19 | 80.1 |
| MS.gene59957.t1 | MTR_8g463180 | 29.577 | 213 | 112 | 6 | 5 | 189 | 7 | 209 | 4.56e-19 | 84.0 |
| MS.gene59957.t1 | MTR_2g090580 | 34.615 | 156 | 93 | 4 | 31 | 185 | 29 | 176 | 4.78e-19 | 82.0 |
| MS.gene59957.t1 | MTR_7g086280 | 31.737 | 167 | 97 | 4 | 10 | 164 | 7 | 168 | 8.78e-19 | 80.9 |
| MS.gene59957.t1 | MTR_6g022170 | 38.679 | 106 | 62 | 2 | 25 | 129 | 30 | 133 | 9.99e-19 | 80.1 |
| MS.gene59957.t1 | MTR_7g086220 | 35.484 | 124 | 65 | 4 | 12 | 129 | 8 | 122 | 1.64e-18 | 79.7 |
| MS.gene59957.t1 | MTR_7g086230 | 32.121 | 165 | 95 | 4 | 12 | 164 | 9 | 168 | 2.58e-18 | 79.3 |
| MS.gene59957.t1 | MTR_4g124280 | 34.211 | 152 | 93 | 3 | 31 | 181 | 27 | 172 | 8.90e-18 | 79.3 |
| MS.gene59957.t1 | MTR_7g086100 | 35.593 | 118 | 72 | 1 | 12 | 129 | 9 | 122 | 1.21e-17 | 79.0 |
| MS.gene59957.t1 | MTR_1g112700 | 29.605 | 152 | 100 | 4 | 4 | 154 | 9 | 154 | 1.46e-17 | 77.8 |
| MS.gene59957.t1 | MTR_4g111640 | 32.192 | 146 | 84 | 4 | 27 | 170 | 30 | 162 | 1.82e-17 | 77.4 |
| MS.gene59957.t1 | MTR_1g090190 | 35.200 | 125 | 74 | 3 | 27 | 149 | 21 | 140 | 2.51e-17 | 77.0 |
| MS.gene59957.t1 | MTR_8g086360 | 33.117 | 154 | 86 | 3 | 31 | 184 | 26 | 162 | 4.86e-17 | 76.6 |
| MS.gene59957.t1 | MTR_4g078410 | 34.694 | 147 | 90 | 4 | 31 | 177 | 27 | 167 | 9.00e-17 | 75.5 |
| MS.gene59957.t1 | MTR_2g090575 | 29.130 | 230 | 131 | 7 | 5 | 213 | 10 | 228 | 2.39e-16 | 77.0 |
| MS.gene59957.t1 | MTR_6g080660 | 32.203 | 118 | 75 | 2 | 11 | 128 | 10 | 122 | 5.29e-16 | 73.9 |
| MS.gene59957.t1 | MTR_8g095013 | 29.353 | 201 | 113 | 6 | 7 | 188 | 7 | 197 | 5.80e-16 | 75.5 |
| MS.gene59957.t1 | MTR_3g099540 | 37.963 | 108 | 62 | 2 | 27 | 134 | 25 | 127 | 6.56e-16 | 72.0 |
| MS.gene59957.t1 | MTR_8g095020 | 31.250 | 192 | 112 | 5 | 31 | 208 | 27 | 212 | 1.33e-15 | 74.7 |
| MS.gene59957.t1 | MTR_8g089110 | 35.537 | 121 | 67 | 3 | 7 | 127 | 10 | 119 | 1.39e-15 | 70.9 |
| MS.gene59957.t1 | MTR_3g089035 | 37.624 | 101 | 56 | 2 | 29 | 129 | 33 | 126 | 1.79e-15 | 70.5 |
| MS.gene59957.t1 | MTR_6g013420 | 35.252 | 139 | 83 | 3 | 8 | 144 | 17 | 150 | 1.98e-15 | 72.0 |
| MS.gene59957.t1 | MTR_4g081100 | 32.000 | 100 | 68 | 0 | 30 | 129 | 33 | 132 | 1.21e-14 | 70.5 |
| MS.gene59957.t1 | MTR_3g105930 | 28.916 | 166 | 96 | 3 | 25 | 190 | 24 | 167 | 1.21e-14 | 69.7 |
| MS.gene59957.t1 | MTR_4g077787 | 30.645 | 124 | 80 | 3 | 27 | 148 | 30 | 149 | 2.24e-14 | 68.9 |
| MS.gene59957.t1 | MTR_6g023760 | 36.893 | 103 | 59 | 3 | 27 | 128 | 24 | 121 | 2.44e-14 | 68.9 |
| MS.gene59957.t1 | MTR_2g079030 | 29.949 | 197 | 123 | 8 | 1 | 192 | 1 | 187 | 2.86e-14 | 71.6 |
| MS.gene59957.t1 | MTR_2g025580 | 28.758 | 153 | 93 | 3 | 9 | 160 | 7 | 144 | 1.15e-13 | 67.0 |
| MS.gene59957.t1 | MTR_2g043790 | 36.190 | 105 | 59 | 3 | 23 | 127 | 2 | 98 | 1.53e-13 | 64.7 |
| MS.gene59957.t1 | MTR_2g083250 | 33.945 | 109 | 70 | 2 | 28 | 134 | 25 | 133 | 1.92e-13 | 67.8 |
| MS.gene59957.t1 | MTR_8g094990 | 29.655 | 145 | 93 | 3 | 1 | 145 | 1 | 136 | 2.68e-13 | 67.8 |
| MS.gene59957.t1 | MTR_3g089005 | 31.746 | 126 | 72 | 4 | 5 | 127 | 10 | 124 | 2.74e-13 | 64.7 |
| MS.gene59957.t1 | MTR_1g077790 | 33.333 | 99 | 65 | 1 | 31 | 128 | 31 | 129 | 2.90e-13 | 66.2 |
| MS.gene59957.t1 | MTR_4g112310 | 31.452 | 124 | 72 | 3 | 47 | 170 | 2 | 112 | 4.36e-13 | 64.3 |
| MS.gene59957.t1 | MTR_4g007250 | 34.314 | 102 | 62 | 2 | 27 | 128 | 27 | 123 | 9.41e-13 | 64.3 |
| MS.gene59957.t1 | MTR_6g013200 | 30.263 | 152 | 91 | 4 | 9 | 158 | 23 | 161 | 1.02e-12 | 64.3 |
| MS.gene59957.t1 | MTR_3g092170 | 26.289 | 194 | 127 | 3 | 1 | 184 | 1 | 188 | 5.91e-12 | 63.9 |
| MS.gene59957.t1 | MTR_6g013170 | 31.538 | 130 | 80 | 4 | 29 | 157 | 31 | 152 | 3.75e-11 | 60.1 |
| MS.gene59957.t1 | MTR_4g130780 | 26.829 | 123 | 87 | 2 | 7 | 128 | 9 | 129 | 4.33e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59957.t1 | AT2G32300 | 41.497 | 147 | 82 | 2 | 9 | 155 | 6 | 148 | 5.38e-32 | 117 |
| MS.gene59957.t1 | AT2G32300 | 41.497 | 147 | 82 | 2 | 9 | 155 | 36 | 178 | 1.71e-31 | 117 |
| MS.gene59957.t1 | AT2G31050 | 39.655 | 174 | 96 | 2 | 10 | 177 | 10 | 180 | 4.60e-31 | 113 |
| MS.gene59957.t1 | AT5G20230 | 46.721 | 122 | 63 | 2 | 31 | 151 | 27 | 147 | 8.03e-30 | 110 |
| MS.gene59957.t1 | AT3G17675 | 51.923 | 104 | 42 | 3 | 27 | 128 | 30 | 127 | 4.84e-29 | 106 |
| MS.gene59957.t1 | AT2G26720 | 40.426 | 141 | 81 | 1 | 1 | 141 | 1 | 138 | 5.28e-29 | 108 |
| MS.gene59957.t1 | AT2G25060 | 38.272 | 162 | 81 | 4 | 13 | 167 | 14 | 163 | 6.32e-25 | 97.1 |
| MS.gene59957.t1 | AT5G26330 | 44.231 | 104 | 56 | 1 | 31 | 134 | 26 | 127 | 2.08e-23 | 93.2 |
| MS.gene59957.t1 | AT4G28365 | 31.053 | 190 | 113 | 6 | 1 | 188 | 5 | 178 | 7.66e-22 | 89.4 |
| MS.gene59957.t1 | AT3G20570 | 33.690 | 187 | 120 | 4 | 1 | 186 | 1 | 184 | 1.16e-21 | 89.0 |
| MS.gene59957.t1 | AT3G60270 | 42.574 | 101 | 53 | 2 | 31 | 131 | 28 | 123 | 1.54e-20 | 85.9 |
| MS.gene59957.t1 | AT4G31840 | 31.056 | 161 | 101 | 1 | 6 | 166 | 5 | 155 | 2.22e-20 | 85.1 |
| MS.gene59957.t1 | AT5G53870 | 34.426 | 183 | 104 | 5 | 10 | 188 | 12 | 182 | 6.43e-20 | 87.0 |
| MS.gene59957.t1 | AT5G53870 | 34.426 | 183 | 104 | 5 | 10 | 188 | 12 | 182 | 9.64e-20 | 86.7 |
| MS.gene59957.t1 | AT3G27200 | 35.878 | 131 | 71 | 5 | 1 | 129 | 3 | 122 | 3.53e-19 | 81.6 |
| MS.gene59957.t1 | AT1G72230 | 32.370 | 173 | 100 | 5 | 4 | 172 | 3 | 162 | 1.03e-18 | 80.9 |
| MS.gene59957.t1 | AT5G07475 | 38.333 | 120 | 67 | 3 | 27 | 144 | 28 | 142 | 2.25e-18 | 80.1 |
| MS.gene59957.t1 | AT4G30590 | 33.803 | 142 | 87 | 3 | 8 | 145 | 10 | 148 | 6.08e-17 | 76.3 |
| MS.gene59957.t1 | AT1G64640 | 31.081 | 148 | 94 | 3 | 8 | 153 | 10 | 151 | 1.34e-16 | 75.5 |
| MS.gene59957.t1 | AT1G22480 | 40.385 | 104 | 57 | 2 | 34 | 137 | 24 | 122 | 1.71e-16 | 74.7 |
| MS.gene59957.t1 | AT2G44790 | 40.909 | 88 | 49 | 2 | 47 | 132 | 42 | 128 | 2.31e-16 | 75.1 |
| MS.gene59957.t1 | AT4G27520 | 32.653 | 147 | 94 | 4 | 3 | 149 | 7 | 148 | 2.57e-16 | 77.0 |
| MS.gene59957.t1 | AT1G79800 | 34.454 | 119 | 71 | 4 | 31 | 145 | 36 | 151 | 3.73e-15 | 71.6 |
| MS.gene59957.t1 | AT3G60280 | 37.624 | 101 | 58 | 2 | 31 | 131 | 26 | 121 | 5.84e-15 | 71.6 |
| MS.gene59957.t1 | AT2G23990 | 31.959 | 194 | 124 | 5 | 8 | 199 | 7 | 194 | 7.40e-15 | 70.9 |
| MS.gene59957.t1 | AT5G14345 | 32.407 | 108 | 70 | 2 | 37 | 143 | 24 | 129 | 4.83e-14 | 67.4 |
| MS.gene59957.t1 | AT2G02850 | 32.787 | 122 | 74 | 3 | 9 | 129 | 15 | 129 | 5.66e-14 | 66.6 |
| MS.gene59957.t1 | AT4G32490 | 30.818 | 159 | 97 | 5 | 31 | 188 | 33 | 179 | 6.12e-14 | 68.6 |
| MS.gene59957.t1 | AT5G25090 | 36.735 | 98 | 62 | 0 | 32 | 129 | 30 | 127 | 1.23e-13 | 67.4 |
| MS.gene59957.t1 | AT3G18590 | 32.000 | 125 | 78 | 4 | 8 | 129 | 7 | 127 | 6.80e-13 | 65.1 |
| MS.gene59957.t1 | AT5G15350 | 33.333 | 117 | 71 | 3 | 28 | 142 | 28 | 139 | 9.64e-13 | 64.7 |
| MS.gene59957.t1 | AT1G48940 | 31.200 | 125 | 81 | 3 | 11 | 132 | 7 | 129 | 3.37e-12 | 63.2 |
| MS.gene59957.t1 | AT5G57920 | 27.193 | 114 | 81 | 1 | 30 | 141 | 25 | 138 | 4.10e-12 | 63.2 |
| MS.gene59957.t1 | AT1G45063 | 24.424 | 217 | 118 | 5 | 9 | 224 | 9 | 180 | 5.60e-12 | 63.9 |
| MS.gene59957.t1 | AT1G45063 | 24.424 | 217 | 118 | 5 | 9 | 224 | 9 | 180 | 8.34e-12 | 63.9 |
| MS.gene59957.t1 | AT5G57920 | 27.193 | 114 | 81 | 1 | 30 | 141 | 24 | 137 | 1.07e-11 | 62.0 |
| MS.gene59957.t1 | AT5G57920 | 27.193 | 114 | 81 | 1 | 30 | 141 | 57 | 170 | 1.28e-11 | 62.4 |
| MS.gene59957.t1 | AT2G27035 | 29.878 | 164 | 100 | 6 | 1 | 157 | 2 | 157 | 5.62e-11 | 59.7 |
Find 66 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTTCATTTGTGCCGTTCC+AGG | 0.347262 | 8.3:-12543574 | MS.gene59957:CDS |
| TGGGGCAGGTGTAGCGGCCT+TGG | 0.370641 | 8.3:+12543411 | None:intergenic |
| GTGCTTCATTCTATACCAAC+TGG | 0.401088 | 8.3:-12543851 | MS.gene59957:CDS |
| TCGTTCAATGTCACGGTAGC+TGG | 0.403067 | 8.3:+12543610 | None:intergenic |
| TCACCGACGGTGTAAGTAAC+AGG | 0.416106 | 8.3:+12543337 | None:intergenic |
| TAAGCAGCAGCACCGTTAGA+TGG | 0.418533 | 8.3:+12543295 | None:intergenic |
| TCTCGCAAGTCTTTCAAAGT+TGG | 0.423148 | 8.3:-12543259 | MS.gene59957:CDS |
| TGTCCAGCTGAACAATGTCC+TGG | 0.428453 | 8.3:+12543556 | None:intergenic |
| ACCGTGACATTGAACGAAAC+AGG | 0.440151 | 8.3:-12543604 | MS.gene59957:CDS |
| CTTGGAGTTGGAGCAGAAAC+AGG | 0.440341 | 8.3:+12543496 | None:intergenic |
| CCTGCTCCAGCTCCTGCTAC+TGG | 0.441017 | 8.3:-12543361 | MS.gene59957:CDS |
| GTGCAAATGATCCATGGAAC+AGG | 0.441402 | 8.3:+12543925 | None:intergenic |
| AGAATGAAGCACCGTTGGTT+GGG | 0.451694 | 8.3:+12543862 | None:intergenic |
| GGTGGAGAAGCACTTGGAGT+TGG | 0.455723 | 8.3:+12543484 | None:intergenic |
| GTAGGAGCTGGAGTAGCCTT+AGG | 0.459132 | 8.3:+12543463 | None:intergenic |
| GCAGGTGTAGCGGCCTTGGT+TGG | 0.463363 | 8.3:+12543415 | None:intergenic |
| AACATTAAGGTTCTTTGCCA+TGG | 0.467663 | 8.3:+12543980 | None:intergenic |
| GTAGTAGATGGTGCTGGGGC+AGG | 0.476078 | 8.3:+12543397 | None:intergenic |
| GAAAGACTTGCGAGAAGCCC+AGG | 0.480465 | 8.3:+12543267 | None:intergenic |
| GCTGGAACAGGGGTAGGAGC+TGG | 0.482904 | 8.3:+12543451 | None:intergenic |
| CCGTCGGTGACACCATTGGC+TGG | 0.488830 | 8.3:-12543324 | MS.gene59957:CDS |
| TAAGTAACAGGTCCAGTAGC+AGG | 0.490680 | 8.3:+12543349 | None:intergenic |
| TTCAAAGTTGGAGACATCCT+TGG | 0.494707 | 8.3:-12543247 | None:intergenic |
| GTTCCAGGACATTGTTCAGC+TGG | 0.499514 | 8.3:-12543559 | MS.gene59957:CDS |
| TCCTGTTTCGTTCAATGTCA+CGG | 0.504654 | 8.3:+12543603 | None:intergenic |
| TAGAATGAAGCACCGTTGGT+TGG | 0.509679 | 8.3:+12543861 | None:intergenic |
| GTAGCGGCCTTGGTTGGTGC+CGG | 0.513837 | 8.3:+12543421 | None:intergenic |
| GGTAGCTGGACTGTTTGTTA+AGG | 0.513870 | 8.3:+12543624 | None:intergenic |
| GTGCTGCTGCTTATACAACC+TGG | 0.514286 | 8.3:-12543285 | MS.gene59957:CDS |
| CCAGTAGCAGGAGCTGGAGC+AGG | 0.514710 | 8.3:+12543361 | None:intergenic |
| ACAGGTCCAGTAGCAGGAGC+TGG | 0.516512 | 8.3:+12543355 | None:intergenic |
| AGCAGCACCGTTAGATGGAA+TGG | 0.523720 | 8.3:+12543300 | None:intergenic |
| GAAGCAGTAGTAGATGGTGC+TGG | 0.524239 | 8.3:+12543391 | None:intergenic |
| TCCAAGTGCTTCTCCACCTA+AGG | 0.524590 | 8.3:-12543479 | MS.gene59957:CDS |
| AAGCAGTAGTAGATGGTGCT+GGG | 0.527160 | 8.3:+12543392 | None:intergenic |
| TGCTGCTGCTTATACAACCT+GGG | 0.535411 | 8.3:-12543284 | MS.gene59957:CDS |
| GGTATAGAATGAAGCACCGT+TGG | 0.538174 | 8.3:+12543857 | None:intergenic |
| AAAAGCACCGGCACCAACCA+AGG | 0.541334 | 8.3:-12543428 | MS.gene59957:CDS |
| TGGTGCTGGGGCAGGTGTAG+CGG | 0.541610 | 8.3:+12543405 | None:intergenic |
| GCAGGAGCTGGAGCAGGTGT+TGG | 0.542881 | 8.3:+12543367 | None:intergenic |
| TACACCGTCGGTGACACCAT+TGG | 0.546097 | 8.3:-12543328 | MS.gene59957:CDS |
| GCCTTAGGTGGAGAAGCACT+TGG | 0.556300 | 8.3:+12543478 | None:intergenic |
| GCAGTGTTCAATTATGCAAG+TGG | 0.556563 | 8.3:-12543715 | MS.gene59957:intron |
| GTTGGTGAAGCAGTAGTAGA+TGG | 0.562949 | 8.3:+12543385 | None:intergenic |
| TGGACAACATGATGTTGCTA+AGG | 0.564994 | 8.3:-12543695 | MS.gene59957:CDS |
| AGATGGAATGGTCCAGCCAA+TGG | 0.572806 | 8.3:+12543312 | None:intergenic |
| TTGTTTGTGCAAATGATCCA+TGG | 0.576582 | 8.3:+12543919 | None:intergenic |
| GGCTGGACCATTCCATCTAA+CGG | 0.581650 | 8.3:-12543307 | MS.gene59957:CDS |
| CCAGCCAATGGTGTCACCGA+CGG | 0.581738 | 8.3:+12543324 | None:intergenic |
| CCCTGTTCCAGCAAAAGCAC+CGG | 0.587423 | 8.3:-12543440 | MS.gene59957:CDS |
| TGCTTCATTCTATACCAACT+GGG | 0.590943 | 8.3:-12543850 | MS.gene59957:CDS |
| ACAGCTTACGATAGTTGCAA+TGG | 0.598738 | 8.3:-12543664 | MS.gene59957:CDS |
| GTTGCTGCAAACCTGTTCCA+TGG | 0.601439 | 8.3:-12543936 | MS.gene59957:CDS |
| TTCACTGTAGGCGACACTCT+CGG | 0.604689 | 8.3:-12543813 | MS.gene59957:intron |
| AGCTGAACAATGTCCTGGAA+CGG | 0.608366 | 8.3:+12543561 | None:intergenic |
| GGACCTGTTACTTACACCGT+CGG | 0.610193 | 8.3:-12543340 | MS.gene59957:CDS |
| GGAGCTGGAGTAGCCTTAGG+TGG | 0.618274 | 8.3:+12543466 | None:intergenic |
| GAGAGTGTCGCCTACAGTGA+AGG | 0.626729 | 8.3:+12543815 | None:intergenic |
| TCCAACAAAACCTTCACTGT+AGG | 0.644441 | 8.3:-12543825 | MS.gene59957:CDS |
| ATTTGCACAAACAAGACATG+TGG | 0.650443 | 8.3:-12543910 | MS.gene59957:CDS |
| CATGTGGTTGGTGATACCAC+AGG | 0.660891 | 8.3:-12543894 | MS.gene59957:CDS |
| GGTTGGACTATCCCAACCAA+CGG | 0.674561 | 8.3:-12543873 | MS.gene59957:CDS |
| AGCAGTAGTAGATGGTGCTG+GGG | 0.674917 | 8.3:+12543393 | None:intergenic |
| GCACAAACAAGACATGTGGT+TGG | 0.685878 | 8.3:-12543906 | MS.gene59957:CDS |
| GGTTGGGATAGTCCAACCTG+TGG | 0.687809 | 8.3:+12543878 | None:intergenic |
| TGGTTGGTGATACCACAGGT+TGG | 0.725223 | 8.3:-12543890 | MS.gene59957:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAAACGAATCAAAATGACA+AGG | + | chr8.3:12543460-12543479 | None:intergenic | 25.0% |
| ! | GAACAAAAACAAGAACATTA+AGG | + | chr8.3:12543262-12543281 | None:intergenic | 25.0% |
| ! | TAAACGAATCAAAATGACAA+GGG | + | chr8.3:12543459-12543478 | None:intergenic | 25.0% |
| ATTTGCACAAACAAGACATG+TGG | - | chr8.3:12543316-12543335 | MS.gene59957:CDS | 35.0% | |
| TGCTTCATTCTATACCAACT+GGG | - | chr8.3:12543376-12543395 | MS.gene59957:CDS | 35.0% | |
| TTGTTTGTGCAAATGATCCA+TGG | + | chr8.3:12543310-12543329 | None:intergenic | 35.0% | |
| ACAGCTTACGATAGTTGCAA+TGG | - | chr8.3:12543562-12543581 | MS.gene59957:CDS | 40.0% | |
| GCAGTGTTCAATTATGCAAG+TGG | - | chr8.3:12543511-12543530 | MS.gene59957:CDS | 40.0% | |
| GTGCTTCATTCTATACCAAC+TGG | - | chr8.3:12543375-12543394 | MS.gene59957:CDS | 40.0% | |
| TCCAACAAAACCTTCACTGT+AGG | - | chr8.3:12543401-12543420 | MS.gene59957:CDS | 40.0% | |
| TCCTGTTTCGTTCAATGTCA+CGG | + | chr8.3:12543626-12543645 | None:intergenic | 40.0% | |
| TCTCGCAAGTCTTTCAAAGT+TGG | - | chr8.3:12543967-12543986 | MS.gene59957:CDS | 40.0% | |
| TGGACAACATGATGTTGCTA+AGG | - | chr8.3:12543531-12543550 | MS.gene59957:CDS | 40.0% | |
| AAGCAGTAGTAGATGGTGCT+GGG | + | chr8.3:12543837-12543856 | None:intergenic | 45.0% | |
| ACCGTGACATTGAACGAAAC+AGG | - | chr8.3:12543622-12543641 | MS.gene59957:CDS | 45.0% | |
| AGCTGAACAATGTCCTGGAA+CGG | + | chr8.3:12543668-12543687 | None:intergenic | 45.0% | |
| GCACAAACAAGACATGTGGT+TGG | - | chr8.3:12543320-12543339 | MS.gene59957:CDS | 45.0% | |
| GTGCAAATGATCCATGGAAC+AGG | + | chr8.3:12543304-12543323 | None:intergenic | 45.0% | |
| TAAGTAACAGGTCCAGTAGC+AGG | + | chr8.3:12543880-12543899 | None:intergenic | 45.0% | |
| ! | AACTTCATTTGTGCCGTTCC+AGG | - | chr8.3:12543652-12543671 | MS.gene59957:CDS | 45.0% |
| ! | GGTAGCTGGACTGTTTGTTA+AGG | + | chr8.3:12543605-12543624 | None:intergenic | 45.0% |
| ! | GTTGGTGAAGCAGTAGTAGA+TGG | + | chr8.3:12543844-12543863 | None:intergenic | 45.0% |
| ! | TGCTGCTGCTTATACAACCT+GGG | - | chr8.3:12543942-12543961 | MS.gene59957:CDS | 45.0% |
| !! | AGAATGAAGCACCGTTGGTT+GGG | + | chr8.3:12543367-12543386 | None:intergenic | 45.0% |
| !! | GCCTACAGTGAAGGTTTTGT+TGG | + | chr8.3:12543405-12543424 | None:intergenic | 45.0% |
| !! | GGTATAGAATGAAGCACCGT+TGG | + | chr8.3:12543372-12543391 | None:intergenic | 45.0% |
| !! | TAGAATGAAGCACCGTTGGT+TGG | + | chr8.3:12543368-12543387 | None:intergenic | 45.0% |
| AGATGGAATGGTCCAGCCAA+TGG | + | chr8.3:12543917-12543936 | None:intergenic | 50.0% | |
| AGCAGCACCGTTAGATGGAA+TGG | + | chr8.3:12543929-12543948 | None:intergenic | 50.0% | |
| CTTGGAGTTGGAGCAGAAAC+AGG | + | chr8.3:12543733-12543752 | None:intergenic | 50.0% | |
| GAAGCAGTAGTAGATGGTGC+TGG | + | chr8.3:12543838-12543857 | None:intergenic | 50.0% | |
| GGCTGGACCATTCCATCTAA+CGG | - | chr8.3:12543919-12543938 | MS.gene59957:CDS | 50.0% | |
| GGTTGGACTATCCCAACCAA+CGG | - | chr8.3:12543353-12543372 | MS.gene59957:CDS | 50.0% | |
| GTTCCAGGACATTGTTCAGC+TGG | - | chr8.3:12543667-12543686 | MS.gene59957:CDS | 50.0% | |
| GTTGCTGCAAACCTGTTCCA+TGG | - | chr8.3:12543290-12543309 | MS.gene59957:CDS | 50.0% | |
| TAAGCAGCAGCACCGTTAGA+TGG | + | chr8.3:12543934-12543953 | None:intergenic | 50.0% | |
| TCACCGACGGTGTAAGTAAC+AGG | + | chr8.3:12543892-12543911 | None:intergenic | 50.0% | |
| TCCAAGTGCTTCTCCACCTA+AGG | - | chr8.3:12543747-12543766 | MS.gene59957:intron | 50.0% | |
| TCGTTCAATGTCACGGTAGC+TGG | + | chr8.3:12543619-12543638 | None:intergenic | 50.0% | |
| TGTCCAGCTGAACAATGTCC+TGG | + | chr8.3:12543673-12543692 | None:intergenic | 50.0% | |
| TTCACTGTAGGCGACACTCT+CGG | - | chr8.3:12543413-12543432 | MS.gene59957:CDS | 50.0% | |
| ! | AGCAGTAGTAGATGGTGCTG+GGG | + | chr8.3:12543836-12543855 | None:intergenic | 50.0% |
| ! | GGACCTGTTACTTACACCGT+CGG | - | chr8.3:12543886-12543905 | MS.gene59957:CDS | 50.0% |
| ! | GTGCTGCTGCTTATACAACC+TGG | - | chr8.3:12543941-12543960 | MS.gene59957:CDS | 50.0% |
| !! | CATGTGGTTGGTGATACCAC+AGG | - | chr8.3:12543332-12543351 | MS.gene59957:CDS | 50.0% |
| !! | TGGTTGGTGATACCACAGGT+TGG | - | chr8.3:12543336-12543355 | MS.gene59957:CDS | 50.0% |
| !!! | GGTTTTGTTGGAAGCCCAGT+TGG | + | chr8.3:12543393-12543412 | None:intergenic | 50.0% |
| GAAAGACTTGCGAGAAGCCC+AGG | + | chr8.3:12543962-12543981 | None:intergenic | 55.0% | |
| GGTTGGGATAGTCCAACCTG+TGG | + | chr8.3:12543351-12543370 | None:intergenic | 55.0% | |
| GTAGGAGCTGGAGTAGCCTT+AGG | + | chr8.3:12543766-12543785 | None:intergenic | 55.0% | |
| TACACCGTCGGTGACACCAT+TGG | - | chr8.3:12543898-12543917 | MS.gene59957:CDS | 55.0% | |
| ! | CCCTGTTCCAGCAAAAGCAC+CGG | - | chr8.3:12543786-12543805 | MS.gene59957:intron | 55.0% |
| ! | CCGGTGCTTTTGCTGGAACA+GGG | + | chr8.3:12543789-12543808 | None:intergenic | 55.0% |
| ! | CGGTGCTTTTGCTGGAACAG+GGG | + | chr8.3:12543788-12543807 | None:intergenic | 55.0% |
| ! | GCTTTTGCTGGAACAGGGGT+AGG | + | chr8.3:12543784-12543803 | None:intergenic | 55.0% |
| !! | AAAAGCACCGGCACCAACCA+AGG | - | chr8.3:12543798-12543817 | MS.gene59957:intron | 55.0% |
| !! | GAGAGTGTCGCCTACAGTGA+AGG | + | chr8.3:12543414-12543433 | None:intergenic | 55.0% |
| !! | GCCTTAGGTGGAGAAGCACT+TGG | + | chr8.3:12543751-12543770 | None:intergenic | 55.0% |
| !! | GGTGGAGAAGCACTTGGAGT+TGG | + | chr8.3:12543745-12543764 | None:intergenic | 55.0% |
| ACAGGTCCAGTAGCAGGAGC+TGG | + | chr8.3:12543874-12543893 | None:intergenic | 60.0% | |
| ! | GCCGGTGCTTTTGCTGGAAC+AGG | + | chr8.3:12543790-12543809 | None:intergenic | 60.0% |
| ! | GGAGCTGGAGTAGCCTTAGG+TGG | + | chr8.3:12543763-12543782 | None:intergenic | 60.0% |
| ! | GTAGTAGATGGTGCTGGGGC+AGG | + | chr8.3:12543832-12543851 | None:intergenic | 60.0% |
| !! | CCAGCCAATGGTGTCACCGA+CGG | + | chr8.3:12543905-12543924 | None:intergenic | 60.0% |
| !!! | GTTGGTGCCGGTGCTTTTGC+TGG | + | chr8.3:12543796-12543815 | None:intergenic | 60.0% |
| CCAGTAGCAGGAGCTGGAGC+AGG | + | chr8.3:12543868-12543887 | None:intergenic | 65.0% | |
| CCGTCGGTGACACCATTGGC+TGG | - | chr8.3:12543902-12543921 | MS.gene59957:CDS | 65.0% | |
| CCTGCTCCAGCTCCTGCTAC+TGG | - | chr8.3:12543865-12543884 | MS.gene59957:CDS | 65.0% | |
| GCAGGAGCTGGAGCAGGTGT+TGG | + | chr8.3:12543862-12543881 | None:intergenic | 65.0% | |
| GCAGGTGTAGCGGCCTTGGT+TGG | + | chr8.3:12543814-12543833 | None:intergenic | 65.0% | |
| GCTGGAACAGGGGTAGGAGC+TGG | + | chr8.3:12543778-12543797 | None:intergenic | 65.0% | |
| ! | GTAGCGGCCTTGGTTGGTGC+CGG | + | chr8.3:12543808-12543827 | None:intergenic | 65.0% |
| ! | TGGTGCTGGGGCAGGTGTAG+CGG | + | chr8.3:12543824-12543843 | None:intergenic | 65.0% |
| TGGGGCAGGTGTAGCGGCCT+TGG | + | chr8.3:12543818-12543837 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 12543248 | 12544000 | 12543248 | ID=MS.gene59957 |
| chr8.3 | mRNA | 12543248 | 12544000 | 12543248 | ID=MS.gene59957.t1;Parent=MS.gene59957 |
| chr8.3 | exon | 12543814 | 12544000 | 12543814 | ID=MS.gene59957.t1.exon1;Parent=MS.gene59957.t1 |
| chr8.3 | CDS | 12543814 | 12544000 | 12543814 | ID=cds.MS.gene59957.t1;Parent=MS.gene59957.t1 |
| chr8.3 | exon | 12543248 | 12543733 | 12543248 | ID=MS.gene59957.t1.exon2;Parent=MS.gene59957.t1 |
| chr8.3 | CDS | 12543248 | 12543733 | 12543248 | ID=cds.MS.gene59957.t1;Parent=MS.gene59957.t1 |
| Gene Sequence |
| Protein sequence |
