AlfalfaGEDB | MS.gene60529

M. sativa cultivar XinJiangDaYe / MS.gene60529

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60529.t1	XP_013467304.1	99	96	1	0	1	96	1	96	3.00E-43	184.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60529.t1	I3S1M4	99.0	96	1	0	1	96	1	96	2.2e-43	184.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04984	MS.gene60529	0.808677	2.75E-50	-1.69E-46
MS.gene050228	MS.gene60529	0.800998	1.11E-48	-1.69E-46
MS.gene050442	MS.gene60529	0.814857	1.24E-51	-1.69E-46
MS.gene050719	MS.gene60529	0.816785	4.62E-52	-1.69E-46
MS.gene050721	MS.gene60529	0.81473	1.33E-51	-1.69E-46
MS.gene050724	MS.gene60529	0.816815	4.55E-52	-1.69E-46
MS.gene050824	MS.gene60529	0.809766	1.61E-50	-1.69E-46
MS.gene051757	MS.gene60529	0.832809	7.65E-56	-1.69E-46
MS.gene052302	MS.gene60529	0.806831	6.79E-50	-1.69E-46
MS.gene052376	MS.gene60529	0.823579	1.28E-53	-1.69E-46
MS.gene052858	MS.gene60529	0.800221	1.60E-48	-1.69E-46
MS.gene052860	MS.gene60529	0.805946	1.04E-49	-1.69E-46
MS.gene054236	MS.gene60529	0.803031	4.23E-49	-1.69E-46
MS.gene054472	MS.gene60529	0.806792	6.93E-50	-1.69E-46
MS.gene054930	MS.gene60529	0.801106	1.05E-48	-1.69E-46
MS.gene057869	MS.gene60529	0.806368	8.51E-50	-1.69E-46
MS.gene058906	MS.gene60529	0.828177	1.04E-54	-1.69E-46
MS.gene059249	MS.gene60529	0.809841	1.55E-50	-1.69E-46
MS.gene059713	MS.gene60529	0.821514	3.88E-53	-1.69E-46
MS.gene060167	MS.gene60529	0.803156	3.99E-49	-1.69E-46
MS.gene060998	MS.gene60529	0.828817	7.28E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60529.t1	MTR_1g048960	98.958	96	1	0	1	96	1	96	3.32e-66	194

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60529.t1	AT1G72020	69.072	97	29	1	1	96	1	97	2.35e-45	141

Find 20 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGGACAAAATGAAGTATC+AGG	0.306703	1.2:-68522996	MS.gene60529:CDS
AAATCCTCTACTCACTGATT+TGG	0.327769	1.2:+68523099	None:intergenic
AGTAGAGGATTTCATTCAAC+TGG	0.411924	1.2:-68523088	MS.gene60529:CDS
CAGCAAAAGAAGACAACATC+TGG	0.443589	1.2:-68522899	MS.gene60529:CDS
AAGAGGATGGGAGGCGGACA+TGG	0.484956	1.2:-68523061	MS.gene60529:CDS
GTGTTCACAGCTTTCAGCAT+TGG	0.501579	1.2:-68522953	MS.gene60529:CDS
TGCATATCACATGTACAACT+TGG	0.529028	1.2:-68523014	MS.gene60529:CDS
TTCAACTGGTGTGAAGAGGA+TGG	0.534302	1.2:-68523074	MS.gene60529:CDS
TTCATTCAACTGGTGTGAAG+AGG	0.561102	1.2:-68523078	MS.gene60529:CDS
GCATATACTCTGGCTCGCTA+TGG	0.570951	1.2:+68523038	None:intergenic
TTGGACAAAATGAAGTATCA+GGG	0.577919	1.2:-68522995	MS.gene60529:CDS
ACTCACTGATTTGGAGATGG+AGG	0.581472	1.2:+68523108	None:intergenic
TCAACTGGTGTGAAGAGGAT+GGG	0.584183	1.2:-68523073	MS.gene60529:CDS
TGGAAAATGACTGCATACAC+AGG	0.585917	1.2:+68522920	None:intergenic
TCTACTCACTGATTTGGAGA+TGG	0.595575	1.2:+68523105	None:intergenic
GGTGTGAAGAGGATGGGAGG+CGG	0.610714	1.2:-68523067	MS.gene60529:CDS
ATCTCCAAATCAGTGAGTAG+AGG	0.617769	1.2:-68523103	MS.gene60529:CDS
TGATATGCATGCATATACTC+TGG	0.624731	1.2:+68523028	None:intergenic
ATAACGATGGCATTGAACAA+CGG	0.641834	1.2:-68523169	None:intergenic
ACTGGTGTGAAGAGGATGGG+AGG	0.670600	1.2:-68523070	MS.gene60529:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TTGGACAAAATGAAGTATCA+GGG	-	chr1.2:68523063-68523082	MS.gene60529:CDS	30.0%
	AGTAGAGGATTTCATTCAAC+TGG	-	chr1.2:68522970-68522989	MS.gene60529:CDS	35.0%
	TGATATGCATGCATATACTC+TGG	+	chr1.2:68523033-68523052	None:intergenic	35.0%
	TGCATATCACATGTACAACT+TGG	-	chr1.2:68523044-68523063	MS.gene60529:CDS	35.0%
!	AAATCCTCTACTCACTGATT+TGG	+	chr1.2:68522962-68522981	None:intergenic	35.0%
!	CTTGGACAAAATGAAGTATC+AGG	-	chr1.2:68523062-68523081	MS.gene60529:CDS	35.0%
	ATCTCCAAATCAGTGAGTAG+AGG	-	chr1.2:68522955-68522974	MS.gene60529:CDS	40.0%
	CAGCAAAAGAAGACAACATC+TGG	-	chr1.2:68523159-68523178	MS.gene60529:CDS	40.0%
	TGGAAAATGACTGCATACAC+AGG	+	chr1.2:68523141-68523160	None:intergenic	40.0%
	TTCATTCAACTGGTGTGAAG+AGG	-	chr1.2:68522980-68522999	MS.gene60529:CDS	40.0%
!	CAGATGTTGTCTTCTTTTGC+TGG	+	chr1.2:68523161-68523180	None:intergenic	40.0%
!!	TCTACTCACTGATTTGGAGA+TGG	+	chr1.2:68522956-68522975	None:intergenic	40.0%
	GTGTTCACAGCTTTCAGCAT+TGG	-	chr1.2:68523105-68523124	MS.gene60529:CDS	45.0%
	TCAACTGGTGTGAAGAGGAT+GGG	-	chr1.2:68522985-68523004	MS.gene60529:CDS	45.0%
	TTCAACTGGTGTGAAGAGGA+TGG	-	chr1.2:68522984-68523003	MS.gene60529:CDS	45.0%
!!	ACTCACTGATTTGGAGATGG+AGG	+	chr1.2:68522953-68522972	None:intergenic	45.0%
!	GCATATACTCTGGCTCGCTA+TGG	+	chr1.2:68523023-68523042	None:intergenic	50.0%
	ACTGGTGTGAAGAGGATGGG+AGG	-	chr1.2:68522988-68523007	MS.gene60529:CDS	55.0%
	AAGAGGATGGGAGGCGGACA+TGG	-	chr1.2:68522997-68523016	MS.gene60529:CDS	60.0%
	GGTGTGAAGAGGATGGGAGG+CGG	-	chr1.2:68522991-68523010	MS.gene60529:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	68522895	68523185	68522895	ID=MS.gene60529
chr1.2	mRNA	68522895	68523185	68522895	ID=MS.gene60529.t1;Parent=MS.gene60529
chr1.2	exon	68522895	68523185	68522895	ID=MS.gene60529.t1.exon1;Parent=MS.gene60529.t1
chr1.2	CDS	68522895	68523185	68522895	ID=cds.MS.gene60529.t1;Parent=MS.gene60529.t1

Gene Sequence	>MS.gene60529 ATGGCATTGAACAACGGTCTCAGATCTGCTGCGAAGCTAATTGCTTCTTCTGAATCCTCCATCTCCAAATCAGTGAGTAGAGGATTTCATTCAACTGGTGTGAAGAGGATGGGAGGCGGACATGGCCATAGCGAGCCAGAGTATATGCATGCATATCACATGTACAACTTGGACAAAATGAAGTATCAGGGTTTGAAAATGTCTCTTGCTGTGTTCACAGCTTTCAGCATTGGTGTTGCAGTTCCTGTGTATGCAGTCATTTTCCAGCAAAAGAAGACAACATCTGGCTGA
Protein sequence	>MS.gene60529.t1 MALNNGLRSAAKLIASSESSISKSVSRGFHSTGVKRMGGGHGHSEPEYMHAYHMYNLDKMKYQGLKMSLAVFTAFSIGVAVPVYAVIFQQKKTTSG