AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60979


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60979.t1 MTR_8g067080 98.182 110 1 1 12 121 299 407 1.10e-66 206
MS.gene60979.t1 MTR_8g067080 98.182 110 1 1 12 121 301 409 3.43e-66 205
MS.gene60979.t1 MTR_8g471110 98.182 110 1 1 12 121 301 409 3.43e-66 205
MS.gene60979.t1 MTR_8g067090 87.387 111 13 1 12 121 291 401 4.10e-51 166
MS.gene60979.t1 MTR_8g471120 87.387 111 13 1 12 121 291 401 4.10e-51 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60979.t1 AT4G17890 66.071 112 36 2 12 121 302 413 8.08e-44 147
MS.gene60979.t1 AT5G46750 66.964 112 35 2 12 121 291 402 2.59e-43 146
MS.gene60979.t1 AT2G35210 69.231 104 30 2 12 113 291 394 4.87e-38 132
MS.gene60979.t1 AT4G17890 63.291 79 27 2 12 88 302 380 1.42e-26 101
MS.gene60979.t1 AT2G35210 64.444 45 15 1 12 55 291 335 8.50e-11 58.2

Find 17 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGTGTCTGTATAAGATTC+AGG 0.288486 8.1:+37459511 MS.gene60979:CDS
CATCCGCATTGGCCTTGTTC+TGG 0.300754 8.1:-37459601 None:intergenic
CTCCTTGATGACAGATCTTC+AGG 0.326640 8.1:+37461142 MS.gene60979:CDS
TATCAATAGAATATCTTTCC+AGG 0.348903 8.1:+37460925 MS.gene60979:CDS
AAGAACATTGCTGGAGAGAC+TGG 0.349107 8.1:+37461095 MS.gene60979:CDS
TCTCTCCAGCAATGTTCTTA+AGG 0.357125 8.1:-37461091 None:intergenic
TCTTCCCTTAAGAACATTGC+TGG 0.364267 8.1:+37461086 MS.gene60979:CDS
CTCTCCAGCAATGTTCTTAA+GGG 0.388884 8.1:-37461090 None:intergenic
GGAAAGATATTCTATTGATA+AGG 0.495577 8.1:-37460922 None:intergenic
AGAACATTGCTGGAGAGACT+GGG 0.589784 8.1:+37461096 MS.gene60979:CDS
AGTTGCTTGAGCATCCGCAT+TGG 0.602475 8.1:-37459612 None:intergenic
TGTCATCAAGGAGGATGCCA+AGG 0.607478 8.1:-37461132 None:intergenic
GTCCTGAAGATCTGTCATCA+AGG 0.612922 8.1:-37461144 None:intergenic
GTCACTAGCAGCAAGATCAA+CGG 0.616043 8.1:-37460900 None:intergenic
CATCAAGGAGGATGCCAAGG+AGG 0.643523 8.1:-37461129 None:intergenic
AGACCAGAACAAGGCCAATG+CGG 0.704868 8.1:+37459598 MS.gene60979:CDS
CTGAAGATCTGTCATCAAGG+AGG 0.709942 8.1:-37461141 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTACTTGATTAAATT+TGG - chr8.1:37460580-37460599 None:intergenic 10.0%
!!! AAATTTAATCAAGTAATTTT+TGG + chr8.1:37460579-37460598 MS.gene60979:intron 10.0%
!! GACTAATATTATTTACATTT+GGG + chr8.1:37459934-37459953 MS.gene60979:intron 15.0%
!! TCAATTATGTATGATATAAA+TGG - chr8.1:37460270-37460289 None:intergenic 15.0%
!! TTAAATATTGATACTTAACT+TGG + chr8.1:37460804-37460823 MS.gene60979:intron 15.0%
!! TTCTATTCGTTATAATTTAT+CGG + chr8.1:37460507-37460526 MS.gene60979:intron 15.0%
!! TTTATATCATACATAATTGA+AGG + chr8.1:37460270-37460289 MS.gene60979:intron 15.0%
!! AAATAATATTAGTCCTTGTA+AGG - chr8.1:37459928-37459947 None:intergenic 20.0%
!! CAAAATAATCTATACTTACA+CGG + chr8.1:37460043-37460062 MS.gene60979:intron 20.0%
!! GGACTAATATTATTTACATT+TGG + chr8.1:37459933-37459952 MS.gene60979:intron 20.0%
!! TATAGATATAGATATGTTGA+GGG + chr8.1:37460647-37460666 MS.gene60979:intron 20.0%
!! TTATAGATATAGATATGTTG+AGG + chr8.1:37460646-37460665 MS.gene60979:intron 20.0%
! AGGTAATCCTTATATTATAC+TGG + chr8.1:37460945-37460964 MS.gene60979:intron 25.0%
! AGTATAATATAAGGATTACC+TGG - chr8.1:37460946-37460965 None:intergenic 25.0%
! ATACTTACACGGAAATTTAA+GGG + chr8.1:37460054-37460073 MS.gene60979:intron 25.0%
! GGAAAGATATTCTATTGATA+AGG - chr8.1:37460925-37460944 None:intergenic 25.0%
! GTTTGACATCAAATTTATCA+GGG - chr8.1:37459824-37459843 None:intergenic 25.0%
! TACATACTAACACTTTGTTA+CGG - chr8.1:37459680-37459699 None:intergenic 25.0%
! TATACTTACACGGAAATTTA+AGG + chr8.1:37460053-37460072 MS.gene60979:intron 25.0%
! TATCAATAGAATATCTTTCC+AGG + chr8.1:37460925-37460944 MS.gene60979:CDS 25.0%
! TGTTTGACATCAAATTTATC+AGG - chr8.1:37459825-37459844 None:intergenic 25.0%
! TTGTGTTCAGAAATAATTTC+AGG - chr8.1:37460296-37460315 None:intergenic 25.0%
! TTTGTGTCCAGTATAATATA+AGG - chr8.1:37460955-37460974 None:intergenic 25.0%
!! AATCTTCTCTTTTCTTTCTT+GGG + chr8.1:37460691-37460710 MS.gene60979:intron 25.0%
!! ATGAATGATATTTTACTCAC+AGG + chr8.1:37460127-37460146 MS.gene60979:intron 25.0%
!! CTATAATATCTGAAATTCCA+TGG - chr8.1:37460632-37460651 None:intergenic 25.0%
!! TAATCTTCTCTTTTCTTTCT+TGG + chr8.1:37460690-37460709 MS.gene60979:intron 25.0%
!! TCTATCCTTTTATATTTCCA+GGG + chr8.1:37460827-37460846 MS.gene60979:intron 25.0%
!! TTAGCATTTTTGCATTATCA+AGG + chr8.1:37459980-37459999 MS.gene60979:intron 25.0%
!!! AAATTTATCAGGGTTGATTT+TGG - chr8.1:37459814-37459833 None:intergenic 25.0%
!!! AAGAGACATATTTTTCAAGA+AGG - chr8.1:37460489-37460508 None:intergenic 25.0%
!!! AGTAATTTTTGGCATCTATT+TGG + chr8.1:37460590-37460609 MS.gene60979:intron 25.0%
!!! ATTTCTTCATCCCAATTTTT+TGG + chr8.1:37459575-37459594 MS.gene60979:CDS 25.0%
!!! TATTAGTCAAATTAGTCTTC+AGG + chr8.1:37459895-37459914 MS.gene60979:intron 25.0%
!!! TTATTCCAGACTTGTTTTAT+AGG + chr8.1:37460168-37460187 MS.gene60979:intron 25.0%
AAAGCATGTTCAAATGCATT+GGG - chr8.1:37460465-37460484 None:intergenic 30.0%
ACGGTAAGTTACTAAAAGAA+AGG - chr8.1:37459661-37459680 None:intergenic 30.0%
ATATAGATATGTTGAGGGAT+TGG + chr8.1:37460652-37460671 MS.gene60979:intron 30.0%
ATGAACCCTGGAAATATAAA+AGG - chr8.1:37460835-37460854 None:intergenic 30.0%
GAAAGAAAAGAGAAGATTAC+CGG - chr8.1:37460689-37460708 None:intergenic 30.0%
TAGTTCCTTAGAGACTAATA+AGG - chr8.1:37459874-37459893 None:intergenic 30.0%
TTCTTTGTATTTGTTCTGTG+TGG + chr8.1:37461003-37461022 MS.gene60979:intron 30.0%
TTTACTAGTAAACACCATTG+TGG + chr8.1:37460240-37460259 MS.gene60979:intron 30.0%
! AAAAGCATGTTCAAATGCAT+TGG - chr8.1:37460466-37460485 None:intergenic 30.0%
! ATATAAATGGTTTGCCACAA+TGG - chr8.1:37460257-37460276 None:intergenic 30.0%
! ATGTCACATACTCTTTTGAT+TGG + chr8.1:37459762-37459781 MS.gene60979:intron 30.0%
! ATTTGCCCAGAAAATTTTGA+TGG - chr8.1:37460019-37460038 None:intergenic 30.0%
! CAAAATTTTCTGGGCAAATT+AGG + chr8.1:37460020-37460039 MS.gene60979:intron 30.0%
! GTCTATCCTTTTATATTTCC+AGG + chr8.1:37460826-37460845 MS.gene60979:intron 30.0%
! GTTTTCTCTTCTGTCAAAAT+GGG + chr8.1:37460411-37460430 MS.gene60979:intron 30.0%
! TCGTTATAATTTATCGGCTA+GGG + chr8.1:37460513-37460532 MS.gene60979:intron 30.0%
! TTACGCCATCAAAATTTTCT+GGG + chr8.1:37460011-37460030 MS.gene60979:intron 30.0%
! TTCGTTATAATTTATCGGCT+AGG + chr8.1:37460512-37460531 MS.gene60979:intron 30.0%
! TTTACGCCATCAAAATTTTC+TGG + chr8.1:37460010-37460029 MS.gene60979:intron 30.0%
!! GTTGATTTTGGAAATTTGGT+AGG - chr8.1:37459802-37459821 None:intergenic 30.0%
!!! CATTTTTTGGTGCTTTAGTA+TGG + chr8.1:37460389-37460408 MS.gene60979:intron 30.0%
ACAAACTTAATGAGCAACGA+AGG + chr8.1:37460970-37460989 MS.gene60979:intron 35.0%
AGTTTCCACTCGTAAACATT+GGG - chr8.1:37460369-37460388 None:intergenic 35.0%
ATATTAGTCCTTGTAAGGTC+TGG - chr8.1:37459923-37459942 None:intergenic 35.0%
ATTCCTAGTTGCAAAGACAT+GGG - chr8.1:37460752-37460771 None:intergenic 35.0%
GTGTAAAGCAAATTAAGCCA+TGG + chr8.1:37460612-37460631 MS.gene60979:intron 35.0%
GTTCTGGTCTCCAAAAAATT+GGG - chr8.1:37459588-37459607 None:intergenic 35.0%
TAGGAATGGAAAAACGAAGA+GGG + chr8.1:37460765-37460784 MS.gene60979:intron 35.0%
TCTGTGTCTGTATAAGATTC+AGG + chr8.1:37459511-37459530 MS.gene60979:CDS 35.0%
TGTGACCTATAAAACAAGTC+TGG - chr8.1:37460176-37460195 None:intergenic 35.0%
TGTTCTGGTCTCCAAAAAAT+TGG - chr8.1:37459589-37459608 None:intergenic 35.0%
TTCCTTAGAGACTAATAAGG+CGG - chr8.1:37459871-37459890 None:intergenic 35.0%
! ATCTTGTTGTGCCTGTAATA+AGG - chr8.1:37461063-37461082 None:intergenic 35.0%
! GGTTTTCTCTTCTGTCAAAA+TGG + chr8.1:37460410-37460429 MS.gene60979:intron 35.0%
! TATAATTTATCGGCTAGGGT+TGG + chr8.1:37460517-37460536 MS.gene60979:intron 35.0%
! TCTTGTTGTGCCTGTAATAA+GGG - chr8.1:37461062-37461081 None:intergenic 35.0%
!! CAGGGTTGATTTTGGAAATT+TGG - chr8.1:37459806-37459825 None:intergenic 35.0%
!!! ACGAGTGGAAACTCATTTTT+TGG + chr8.1:37460376-37460395 MS.gene60979:intron 35.0%
!!! ATTTTTTGGAGACCAGAACA+AGG + chr8.1:37459589-37459608 MS.gene60979:CDS 35.0%
ATATTTAACTCCCTCTCTCC+CGG - chr8.1:37460792-37460811 None:intergenic 40.0%
CATGTCTTTGCAACTAGGAA+TGG + chr8.1:37460751-37460770 MS.gene60979:intron 40.0%
CATTCCTAGTTGCAAAGACA+TGG - chr8.1:37460753-37460772 None:intergenic 40.0%
CTAGGAATGGAAAAACGAAG+AGG + chr8.1:37460764-37460783 MS.gene60979:intron 40.0%
CTCTCCAGCAATGTTCTTAA+GGG - chr8.1:37461093-37461112 None:intergenic 40.0%
GAGTTTCCACTCGTAAACAT+TGG - chr8.1:37460370-37460389 None:intergenic 40.0%
TAATCCCTACAGAGTACAGT+CGG - chr8.1:37460216-37460235 None:intergenic 40.0%
TCCTTAGAGACTAATAAGGC+GGG - chr8.1:37459870-37459889 None:intergenic 40.0%
TCTCTCCAGCAATGTTCTTA+AGG - chr8.1:37461094-37461113 None:intergenic 40.0%
TCTTCCCTTAAGAACATTGC+TGG + chr8.1:37461086-37461105 MS.gene60979:CDS 40.0%
TGTAACCCAATGTTTACGAG+TGG + chr8.1:37460361-37460380 MS.gene60979:intron 40.0%
! AAAAAGATCAGCACTGGAGA+TGG - chr8.1:37460861-37460880 None:intergenic 40.0%
! TGTTCTGTGTGGATGTTTTG+TGG + chr8.1:37461014-37461033 MS.gene60979:intron 40.0%
!!! ATCTCCAGTGCTGATCTTTT+TGG + chr8.1:37460860-37460879 MS.gene60979:CDS 40.0%
AAGAACATTGCTGGAGAGAC+TGG + chr8.1:37461095-37461114 MS.gene60979:CDS 45.0%
AGAACATTGCTGGAGAGACT+GGG + chr8.1:37461096-37461115 MS.gene60979:CDS 45.0%
AGATGTTCAATGCCCTGTAG+TGG + chr8.1:37459716-37459735 MS.gene60979:intron 45.0%
CTGAAGATCTGTCATCAAGG+AGG - chr8.1:37461144-37461163 None:intergenic 45.0%
GTCACCAAAAAGATCAGCAC+TGG - chr8.1:37460867-37460886 None:intergenic 45.0%
GTCACTAGCAGCAAGATCAA+CGG - chr8.1:37460903-37460922 None:intergenic 45.0%
GTCCTGAAGATCTGTCATCA+AGG - chr8.1:37461147-37461166 None:intergenic 45.0%
GTTGTGCCTGTAATAAGGGA+GGG - chr8.1:37461058-37461077 None:intergenic 45.0%
TATTTAACTCCCTCTCTCCC+GGG - chr8.1:37460791-37460810 None:intergenic 45.0%
TCTCCCATGTCTTTGCAACT+AGG + chr8.1:37460746-37460765 MS.gene60979:intron 45.0%
TGGGAAAAAACTCACCTCCT+TGG + chr8.1:37461115-37461134 MS.gene60979:CDS 45.0%
TGTTGTGCCTGTAATAAGGG+AGG - chr8.1:37461059-37461078 None:intergenic 45.0%
TTGTGCCTGTAATAAGGGAG+GGG - chr8.1:37461057-37461076 None:intergenic 45.0%
! ATCCAATACAGTGCCACTAC+AGG - chr8.1:37459732-37459751 None:intergenic 45.0%
! CTCCTTGATGACAGATCTTC+AGG + chr8.1:37461142-37461161 MS.gene60979:CDS 45.0%
! GGGATTGGTAAGCTATGTTC+CGG + chr8.1:37460667-37460686 MS.gene60979:intron 45.0%
! TCCAATACAGTGCCACTACA+GGG - chr8.1:37459731-37459750 None:intergenic 45.0%
! TCCCGCCTTATTAGTCTCTA+AGG + chr8.1:37459866-37459885 MS.gene60979:intron 45.0%
! TGTAATAAGGGAGGGGTGTA+GGG - chr8.1:37461050-37461069 None:intergenic 45.0%
!! TATACAATTAATTAATATTA+AGG - chr8.1:37460337-37460356 None:intergenic 5.0%
AAGGCGGGACTTCTGTATTC+AGG - chr8.1:37459855-37459874 None:intergenic 50.0%
AGACCAGAACAAGGCCAATG+CGG + chr8.1:37459598-37459617 MS.gene60979:CDS 50.0%
AGTTGCTTGAGCATCCGCAT+TGG - chr8.1:37459615-37459634 None:intergenic 50.0%
ATGGAAAAACGAAGAGGGCC+CGG + chr8.1:37460770-37460789 MS.gene60979:intron 50.0%
CTACACCCCTCCCTTATTAC+AGG + chr8.1:37461049-37461068 MS.gene60979:intron 50.0%
CTGTCCGACTGTACTCTGTA+GGG + chr8.1:37460209-37460228 MS.gene60979:intron 50.0%
TCTGTCCGACTGTACTCTGT+AGG + chr8.1:37460208-37460227 MS.gene60979:intron 50.0%
TGTCATCAAGGAGGATGCCA+AGG - chr8.1:37461135-37461154 None:intergenic 50.0%
TTCAGGCACCAGACCTTACA+AGG + chr8.1:37459912-37459931 MS.gene60979:intron 50.0%
! CTGTAATAAGGGAGGGGTGT+AGG - chr8.1:37461051-37461070 None:intergenic 50.0%
CATCAAGGAGGATGCCAAGG+AGG - chr8.1:37461132-37461151 None:intergenic 55.0%
GAGTGGGATGAGTGCGAGAA+AGG + chr8.1:37460542-37460561 MS.gene60979:intron 55.0%
GCCCTGTAGTGGCACTGTAT+TGG + chr8.1:37459727-37459746 MS.gene60979:intron 55.0%
TGGAAAAACGAAGAGGGCCC+GGG + chr8.1:37460771-37460790 MS.gene60979:intron 55.0%
TGGAGATGGCAGATGAACCC+TGG - chr8.1:37460847-37460866 None:intergenic 55.0%
!! ATCGGCTAGGGTTGGTTGAG+TGG + chr8.1:37460525-37460544 MS.gene60979:intron 55.0%
!! CATCCGCATTGGCCTTGTTC+TGG - chr8.1:37459604-37459623 None:intergenic 55.0%
!! TCGGCTAGGGTTGGTTGAGT+GGG + chr8.1:37460526-37460545 MS.gene60979:intron 55.0%
ACGAAGAGGGCCCGGGAGAG+AGG + chr8.1:37460778-37460797 MS.gene60979:intron 70.0%
CGAAGAGGGCCCGGGAGAGA+GGG + chr8.1:37460779-37460798 MS.gene60979:intron 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 37459491 37461178 37459491 ID=MS.gene60979
chr8.1 mRNA 37459491 37461178 37459491 ID=MS.gene60979.t1;Parent=MS.gene60979
chr8.1 exon 37459491 37459649 37459491 ID=MS.gene60979.t1.exon1;Parent=MS.gene60979.t1
chr8.1 CDS 37459491 37459649 37459491 ID=cds.MS.gene60979.t1;Parent=MS.gene60979.t1
chr8.1 exon 37460848 37460946 37460848 ID=MS.gene60979.t1.exon2;Parent=MS.gene60979.t1
chr8.1 CDS 37460848 37460946 37460848 ID=cds.MS.gene60979.t1;Parent=MS.gene60979.t1
chr8.1 exon 37461071 37461178 37461071 ID=MS.gene60979.t1.exon3;Parent=MS.gene60979.t1
chr8.1 CDS 37461071 37461178 37461071 ID=cds.MS.gene60979.t1;Parent=MS.gene60979.t1
Gene Sequence

>MS.gene60979

ATGCATCTCATTTTGCTTTGTCTGTGTCTGTATAAGATTCAGGAATCTGATGAAGCAAGAAAGAAGTTCTCAAATGCAAAGTCTATTTCTTCATCCCAATTTTTTGGAGACCAGAACAAGGCCAATGCGGATGCTCAAGCAACTTTATCAAAGTTTTCAGTATGTGTCCTTTCTTTTAGTAACTTACCGTAACAAAGTGTTAGTATGTATGCACATGTAGATCATAGATGTTCAATGCCCTGTAGTGGCACTGTATTGGATGACTTCTTTCATGTCACATACTCTTTTGATTGGCAGAATTTATATTACCTACCAAATTTCCAAAATCAACCCTGATAAATTTGATGTCAAACACTCTAATCCTGAATACAGAAGTCCCGCCTTATTAGTCTCTAAGGAACTAATATTAGTCAAATTAGTCTTCAGGCACCAGACCTTACAAGGACTAATATTATTTACATTTGGGATTCATGCATATTAGTATTTAAATTAGCATTTTTGCATTATCAAGGATCACTATTTACGCCATCAAAATTTTCTGGGCAAATTAGGCAAAATAATCTATACTTACACGGAAATTTAAGGGCTAGTGATCTATGTTGTTTTGAAGTGAATATTTCATAATGAAGATAAAATATGAATGATATTTTACTCACAGGTGTTTTTGTAAAGATAAGTTATTCCAGACTTGTTTTATAGGTCACAGTTTTTTTTTCTTCTGTCCGACTGTACTCTGTAGGGATTACAGTTTTACTAGTAAACACCATTGTGGCAAACCATTTATATCATACATAATTGAAGGCCTGAAATTATTTCTGAACACAATGTCTGCGAATTTTTTTGCCTTAATATTAATTAATTGTATAATCTTGTAACCCAATGTTTACGAGTGGAAACTCATTTTTTGGTGCTTTAGTATGGTTTTCTCTTCTGTCAAAATGGGTTGTAGTGCATCAATTTACAGAATTTAACCCAATGCATTTGAACATGCTTTTCCTTCTTGAAAAATATGTCTCTTCTATTCGTTATAATTTATCGGCTAGGGTTGGTTGAGTGGGATGAGTGCGAGAAAGGACAAGTTATGTTCCAAATTTAATCAAGTAATTTTTGGCATCTATTTGGTGTAAAGCAAATTAAGCCATGGAATTTCAGATATTATAGATATAGATATGTTGAGGGATTGGTAAGCTATGTTCCGGTAATCTTCTCTTTTCTTTCTTGGGTATTTTTGTCTTTGTTTTGAAGAAGTTCTTATTCTCCCATGTCTTTGCAACTAGGAATGGAAAAACGAAGAGGGCCCGGGAGAGAGGGAGTTAAATATTGATACTTAACTTGGTCTATCCTTTTATATTTCCAGGGTTCATCTGCCATCTCCAGTGCTGATCTTTTTGGTGACTCGAGTGACAACTCCGTTGATCTTGCTGCTAGTGACCTTATCAATAGAATATCTTTCCAGGTAATCCTTATATTATACTGGACACAAACTTAATGAGCAACGAAGGCATTATTAGATTCTTTGTATTTGTTCTGTGTGGATGTTTTGTGGTATGCTGATACCCTACACCCCTCCCTTATTACAGGCACAACAAGATATCTCTTCCCTTAAGAACATTGCTGGAGAGACTGGGAAAAAACTCACCTCCTTGGCATCCTCCTTGATGACAGATCTTCAGGACCGAATCCTCTAA

Protein sequence

>MS.gene60979.t1

MHLILLCLCLYKIQESDEARKKFSNAKSISSSQFFGDQNKANADAQATLSKFSGSSAISSADLFGDSSDNSVDLAASDLINRISFQAQQDISSLKNIAGETGKKLTSLASSLMTDLQDRIL