Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61928.t1 | XP_003615419.1 | 92.1 | 151 | 11 | 1 | 1 | 151 | 1 | 150 | 1.40E-71 | 278.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61928.t1 | G7KD75 | 92.1 | 151 | 11 | 1 | 1 | 151 | 1 | 150 | 1.0e-71 | 278.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene61928 | 0.834813 | 2.41E-56 | -1.69E-46 |
| MS.gene050654 | MS.gene61928 | 0.804218 | 2.40E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene61928 | 0.827156 | 1.83E-54 | -1.69E-46 |
| MS.gene050945 | MS.gene61928 | 0.834699 | 2.58E-56 | -1.69E-46 |
| MS.gene050955 | MS.gene61928 | 0.812178 | 4.83E-51 | -1.69E-46 |
| MS.gene051003 | MS.gene61928 | 0.816941 | 4.27E-52 | -1.69E-46 |
| MS.gene051043 | MS.gene61928 | 0.813108 | 3.02E-51 | -1.69E-46 |
| MS.gene051275 | MS.gene61928 | 0.808747 | 2.66E-50 | -1.69E-46 |
| MS.gene051343 | MS.gene61928 | 0.832972 | 6.96E-56 | -1.69E-46 |
| MS.gene051902 | MS.gene61928 | 0.813546 | 2.42E-51 | -1.69E-46 |
| MS.gene051975 | MS.gene61928 | 0.80431 | 2.30E-49 | -1.69E-46 |
| MS.gene053058 | MS.gene61928 | 0.82534 | 4.94E-54 | -1.69E-46 |
| MS.gene053564 | MS.gene61928 | 0.815099 | 1.10E-51 | -1.69E-46 |
| MS.gene053931 | MS.gene61928 | 0.823192 | 1.58E-53 | -1.69E-46 |
| MS.gene054403 | MS.gene61928 | 0.830618 | 2.65E-55 | -1.69E-46 |
| MS.gene057208 | MS.gene61928 | 0.804145 | 2.49E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene61928 | 0.804412 | 2.19E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene61928 | 0.830012 | 3.73E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61928.t1 | MTR_5g067740 | 92.053 | 151 | 11 | 1 | 1 | 151 | 1 | 150 | 1.37e-99 | 283 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61928.t1 | AT5G05220 | 56.250 | 64 | 26 | 1 | 90 | 151 | 71 | 134 | 7.22e-18 | 76.3 |
| MS.gene61928.t1 | AT5G05220 | 61.364 | 44 | 17 | 0 | 107 | 150 | 136 | 179 | 1.24e-12 | 62.4 |
Find 33 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGTGGGATGTGCTAGTT+GGG | 0.317379 | 5.2:-57584007 | MS.gene61928:CDS |
| TTTGGAAAAGGAAGCAATAA+TGG | 0.320210 | 5.2:-57583854 | MS.gene61928:CDS |
| GGAATTTCTCATAAACAAAA+AGG | 0.325228 | 5.2:-57584042 | MS.gene61928:CDS |
| TGGAGAAAGGGAAGGGGTTG+TGG | 0.334523 | 5.2:-57583890 | MS.gene61928:CDS |
| GGTTTCAATGTCACATTACT+AGG | 0.361956 | 5.2:+57584081 | None:intergenic |
| TGAAGTATTTCAAGCAGTTA+AGG | 0.368214 | 5.2:-57583767 | MS.gene61928:CDS |
| TGAGATTGATGATGAAGAAA+AGG | 0.384552 | 5.2:-57583947 | MS.gene61928:CDS |
| GATGAAGAAAAGGGTAAAAG+TGG | 0.435549 | 5.2:-57583937 | MS.gene61928:CDS |
| ATTCCCACCTTTGTCCTCAA+TGG | 0.437329 | 5.2:+57584060 | None:intergenic |
| GGGATGTGCTAGTTGGGGAT+TGG | 0.446491 | 5.2:-57584001 | MS.gene61928:CDS |
| GAGATTGATGATGAAGAAAA+GGG | 0.457481 | 5.2:-57583946 | MS.gene61928:CDS |
| TAGAGAAAAGTGTGGAGAAA+GGG | 0.459998 | 5.2:-57583902 | MS.gene61928:CDS |
| TTGTTGTGGGATGTGCTAGT+TGG | 0.465218 | 5.2:-57584008 | MS.gene61928:CDS |
| ATAGAGAAAAGTGTGGAGAA+AGG | 0.467324 | 5.2:-57583903 | MS.gene61928:CDS |
| GCCCTTCGATTGTAATCATT+AGG | 0.469511 | 5.2:+57583811 | None:intergenic |
| AGGTAAAAGGGAATTTGTTG+TGG | 0.499813 | 5.2:-57584022 | MS.gene61928:CDS |
| TTGGAAAAGGAAGCAATAAT+GGG | 0.513066 | 5.2:-57583853 | MS.gene61928:CDS |
| AAAAGTGTGGAGAAAGGGAA+GGG | 0.513638 | 5.2:-57583897 | MS.gene61928:CDS |
| GGTAAAAGGGAATTTGTTGT+GGG | 0.517108 | 5.2:-57584021 | MS.gene61928:CDS |
| AGCCTAATGATTACAATCGA+AGG | 0.534075 | 5.2:-57583813 | MS.gene61928:CDS |
| GCAATAATGGGTGATGATGA+AGG | 0.538475 | 5.2:-57583841 | MS.gene61928:CDS |
| ATCATGAACATTAGCAACAA+AGG | 0.562063 | 5.2:+57584167 | None:intergenic |
| GAAAAGTGTGGAGAAAGGGA+AGG | 0.573580 | 5.2:-57583898 | MS.gene61928:CDS |
| AAAGTGTGGAGAAAGGGAAG+GGG | 0.577573 | 5.2:-57583896 | MS.gene61928:CDS |
| GAAACCATTGAGGACAAAGG+TGG | 0.582861 | 5.2:-57584064 | MS.gene61928:CDS |
| GAGTTAGAACAAATCAAAGA+AGG | 0.587741 | 5.2:+57584132 | None:intergenic |
| ACAAGGTATAGAGAAAAGTG+TGG | 0.590729 | 5.2:-57583910 | MS.gene61928:CDS |
| GCCTAATGATTACAATCGAA+GGG | 0.594969 | 5.2:-57583812 | MS.gene61928:CDS |
| GTTGTGGGATGTGCTAGTTG+GGG | 0.607040 | 5.2:-57584006 | MS.gene61928:CDS |
| AGGGTAAAAGTGGAATAACA+AGG | 0.635782 | 5.2:-57583927 | MS.gene61928:CDS |
| AAACCATTGAGGACAAAGGT+GGG | 0.637541 | 5.2:-57584063 | MS.gene61928:CDS |
| ATGTGACATTGAAACCATTG+AGG | 0.645312 | 5.2:-57584074 | MS.gene61928:CDS |
| ATTGAAACCATTGAGGACAA+AGG | 0.674535 | 5.2:-57584067 | MS.gene61928:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATAAACAAAAAGGTAAAA+GGG | - | chr5.2:57583893-57583912 | MS.gene61928:CDS | 20.0% |
| ! | CTCATAAACAAAAAGGTAAA+AGG | - | chr5.2:57583892-57583911 | MS.gene61928:CDS | 25.0% |
| ! | GGAATTTCTCATAAACAAAA+AGG | - | chr5.2:57583885-57583904 | MS.gene61928:CDS | 25.0% |
| ! | TACTAGGAAAAACATTACTT+TGG | + | chr5.2:57583833-57583852 | None:intergenic | 25.0% |
| ATCATGAACATTAGCAACAA+AGG | + | chr5.2:57583763-57583782 | None:intergenic | 30.0% | |
| GAGTTAGAACAAATCAAAGA+AGG | + | chr5.2:57583798-57583817 | None:intergenic | 30.0% | |
| TTGGAAAAGGAAGCAATAAT+GGG | - | chr5.2:57584074-57584093 | MS.gene61928:CDS | 30.0% | |
| TTTGGAAAAGGAAGCAATAA+TGG | - | chr5.2:57584073-57584092 | MS.gene61928:CDS | 30.0% | |
| ! | GGAAAAACATTACTTTGGAA+TGG | + | chr5.2:57583828-57583847 | None:intergenic | 30.0% |
| ! | TGAAGTATTTCAAGCAGTTA+AGG | - | chr5.2:57584160-57584179 | MS.gene61928:CDS | 30.0% |
| !! | GAGATTGATGATGAAGAAAA+GGG | - | chr5.2:57583981-57584000 | MS.gene61928:CDS | 30.0% |
| !! | TGAGATTGATGATGAAGAAA+AGG | - | chr5.2:57583980-57583999 | MS.gene61928:CDS | 30.0% |
| ACAAGGTATAGAGAAAAGTG+TGG | - | chr5.2:57584017-57584036 | MS.gene61928:CDS | 35.0% | |
| AGCCTAATGATTACAATCGA+AGG | - | chr5.2:57584114-57584133 | MS.gene61928:CDS | 35.0% | |
| AGGGTAAAAGTGGAATAACA+AGG | - | chr5.2:57584000-57584019 | MS.gene61928:CDS | 35.0% | |
| ATGTGACATTGAAACCATTG+AGG | - | chr5.2:57583853-57583872 | MS.gene61928:CDS | 35.0% | |
| ATTGAAACCATTGAGGACAA+AGG | - | chr5.2:57583860-57583879 | MS.gene61928:CDS | 35.0% | |
| GATGAAGAAAAGGGTAAAAG+TGG | - | chr5.2:57583990-57584009 | MS.gene61928:CDS | 35.0% | |
| GCCTAATGATTACAATCGAA+GGG | - | chr5.2:57584115-57584134 | MS.gene61928:CDS | 35.0% | |
| GGTTTCAATGTCACATTACT+AGG | + | chr5.2:57583849-57583868 | None:intergenic | 35.0% | |
| ! | ATAGAGAAAAGTGTGGAGAA+AGG | - | chr5.2:57584024-57584043 | MS.gene61928:CDS | 35.0% |
| ! | TAGAGAAAAGTGTGGAGAAA+GGG | - | chr5.2:57584025-57584044 | MS.gene61928:CDS | 35.0% |
| !! | AGGTAAAAGGGAATTTGTTG+TGG | - | chr5.2:57583905-57583924 | MS.gene61928:CDS | 35.0% |
| !! | GGTAAAAGGGAATTTGTTGT+GGG | - | chr5.2:57583906-57583925 | MS.gene61928:CDS | 35.0% |
| !!! | GTTTTTGGAGTGTTTGGAAA+AGG | - | chr5.2:57584061-57584080 | MS.gene61928:CDS | 35.0% |
| AAACCATTGAGGACAAAGGT+GGG | - | chr5.2:57583864-57583883 | MS.gene61928:CDS | 40.0% | |
| GCCCTTCGATTGTAATCATT+AGG | + | chr5.2:57584119-57584138 | None:intergenic | 40.0% | |
| ! | AAAAGTGTGGAGAAAGGGAA+GGG | - | chr5.2:57584030-57584049 | MS.gene61928:CDS | 40.0% |
| !! | GCAATAATGGGTGATGATGA+AGG | - | chr5.2:57584086-57584105 | MS.gene61928:CDS | 40.0% |
| !!! | TGTGGAGTTTTTGGAGTGTT+TGG | - | chr5.2:57584055-57584074 | MS.gene61928:CDS | 40.0% |
| AAAGTGTGGAGAAAGGGAAG+GGG | - | chr5.2:57584031-57584050 | MS.gene61928:CDS | 45.0% | |
| ATTCCCACCTTTGTCCTCAA+TGG | + | chr5.2:57583870-57583889 | None:intergenic | 45.0% | |
| GAAACCATTGAGGACAAAGG+TGG | - | chr5.2:57583863-57583882 | MS.gene61928:CDS | 45.0% | |
| TGTTGTGGGATGTGCTAGTT+GGG | - | chr5.2:57583920-57583939 | MS.gene61928:CDS | 45.0% | |
| TTGTTGTGGGATGTGCTAGT+TGG | - | chr5.2:57583919-57583938 | MS.gene61928:CDS | 45.0% | |
| ! | GAAAAGTGTGGAGAAAGGGA+AGG | - | chr5.2:57584029-57584048 | MS.gene61928:CDS | 45.0% |
| GTTGTGGGATGTGCTAGTTG+GGG | - | chr5.2:57583921-57583940 | MS.gene61928:CDS | 50.0% | |
| ! | GGAAGGGGTTGTGGAGTTTT+TGG | - | chr5.2:57584046-57584065 | MS.gene61928:CDS | 50.0% |
| GGGATGTGCTAGTTGGGGAT+TGG | - | chr5.2:57583926-57583945 | MS.gene61928:CDS | 55.0% | |
| TGGAGAAAGGGAAGGGGTTG+TGG | - | chr5.2:57584037-57584056 | MS.gene61928:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 57583747 | 57584202 | 57583747 | ID=MS.gene61928 |
| chr5.2 | mRNA | 57583747 | 57584202 | 57583747 | ID=MS.gene61928.t1;Parent=MS.gene61928 |
| chr5.2 | exon | 57583747 | 57584202 | 57583747 | ID=MS.gene61928.t1.exon1;Parent=MS.gene61928.t1 |
| chr5.2 | CDS | 57583747 | 57584202 | 57583747 | ID=cds.MS.gene61928.t1;Parent=MS.gene61928.t1 |
| Gene Sequence |
| Protein sequence |
