Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62904.t1 | XP_003608704.1 | 89.5 | 324 | 25 | 4 | 1 | 324 | 1 | 315 | 2.50E-105 | 392.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62904.t1 | Q9M202 | 30.1 | 332 | 175 | 7 | 1 | 321 | 1 | 286 | 1.4e-32 | 141.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62904.t1 | G7JIV8 | 89.5 | 324 | 25 | 4 | 1 | 324 | 1 | 315 | 1.8e-105 | 392.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene62904.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene62904 | 0.818046 | 2.40E-52 | -1.69E-46 |
| MS.gene049404 | MS.gene62904 | 0.812978 | 3.23E-51 | -1.69E-46 |
| MS.gene050561 | MS.gene62904 | 0.805669 | 1.19E-49 | -1.69E-46 |
| MS.gene050657 | MS.gene62904 | 0.8067 | 7.24E-50 | -1.69E-46 |
| MS.gene051198 | MS.gene62904 | 0.811751 | 5.99E-51 | -1.69E-46 |
| MS.gene051279 | MS.gene62904 | 0.836452 | 9.28E-57 | -1.69E-46 |
| MS.gene051744 | MS.gene62904 | 0.864333 | 1.31E-64 | -1.69E-46 |
| MS.gene052037 | MS.gene62904 | 0.80031 | 1.53E-48 | -1.69E-46 |
| MS.gene052516 | MS.gene62904 | 0.801503 | 8.73E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene62904 | 0.805135 | 1.54E-49 | -1.69E-46 |
| MS.gene052584 | MS.gene62904 | 0.820221 | 7.69E-53 | -1.69E-46 |
| MS.gene053079 | MS.gene62904 | 0.808801 | 2.59E-50 | -1.69E-46 |
| MS.gene053144 | MS.gene62904 | 0.813403 | 2.61E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene62904 | 0.833028 | 6.75E-56 | -1.69E-46 |
| MS.gene053296 | MS.gene62904 | 0.824795 | 6.65E-54 | -1.69E-46 |
| MS.gene053621 | MS.gene62904 | 0.808752 | 2.65E-50 | -1.69E-46 |
| MS.gene054576 | MS.gene62904 | 0.822536 | 2.25E-53 | -1.69E-46 |
| MS.gene054584 | MS.gene62904 | 0.813002 | 3.19E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene62904 | 0.842503 | 2.49E-58 | -1.69E-46 |
| MS.gene055586 | MS.gene62904 | 0.804754 | 1.86E-49 | -1.69E-46 |
| MS.gene056050 | MS.gene62904 | 0.81326 | 2.80E-51 | -1.69E-46 |
| MS.gene056595 | MS.gene62904 | 0.801218 | 9.99E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene62904 | 0.803551 | 3.30E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene62904 | 0.81943 | 1.17E-52 | -1.69E-46 |
| MS.gene058010 | MS.gene62904 | 0.810173 | 1.31E-50 | -1.69E-46 |
| MS.gene059570 | MS.gene62904 | 0.814126 | 1.81E-51 | -1.69E-46 |
| MS.gene059601 | MS.gene62904 | 0.804717 | 1.89E-49 | -1.69E-46 |
| MS.gene060373 | MS.gene62904 | 0.808534 | 2.95E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62904.t1 | MTR_4g100860 | 93.210 | 324 | 13 | 4 | 1 | 324 | 1 | 315 | 0.0 | 579 |
| MS.gene62904.t1 | MTR_0160s0030 | 41.509 | 371 | 154 | 13 | 1 | 324 | 1 | 355 | 6.43e-66 | 211 |
| MS.gene62904.t1 | MTR_5g012630 | 41.718 | 326 | 106 | 13 | 1 | 309 | 1 | 259 | 2.49e-57 | 186 |
| MS.gene62904.t1 | MTR_4g027440 | 34.877 | 367 | 162 | 8 | 1 | 312 | 2 | 346 | 2.52e-43 | 153 |
| MS.gene62904.t1 | MTR_1g106730 | 30.952 | 168 | 78 | 6 | 160 | 314 | 57 | 199 | 1.37e-11 | 63.9 |
| MS.gene62904.t1 | MTR_4g109340 | 29.167 | 216 | 85 | 8 | 3 | 176 | 10 | 199 | 6.86e-11 | 63.5 |
| MS.gene62904.t1 | MTR_3g102980 | 29.714 | 175 | 87 | 6 | 155 | 310 | 44 | 201 | 9.23e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62904.t1 | AT3G60580 | 36.310 | 336 | 149 | 11 | 1 | 321 | 1 | 286 | 1.12e-47 | 162 |
| MS.gene62904.t1 | AT1G02030 | 35.417 | 336 | 129 | 11 | 2 | 321 | 3 | 266 | 5.63e-41 | 144 |
| MS.gene62904.t1 | AT2G45120 | 34.985 | 343 | 172 | 10 | 1 | 323 | 1 | 312 | 1.49e-39 | 142 |
| MS.gene62904.t1 | AT4G16610 | 38.372 | 172 | 85 | 4 | 3 | 173 | 4 | 155 | 2.12e-24 | 98.6 |
| MS.gene62904.t1 | AT1G26610 | 46.753 | 77 | 34 | 2 | 238 | 314 | 386 | 455 | 5.96e-12 | 66.6 |
| MS.gene62904.t1 | AT5G61470 | 43.902 | 82 | 36 | 3 | 205 | 276 | 218 | 299 | 3.32e-11 | 63.5 |
Find 48 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTGTTGTAGAAAATTC+TGG | 0.230051 | 8.1:+22378163 | None:intergenic |
| CCAAACACCTTAAAACAAAA+TGG | 0.311153 | 8.1:+22377772 | None:intergenic |
| TCACGTACAAAAGAAAATTC+AGG | 0.344635 | 8.1:+22378303 | None:intergenic |
| CACATAGCAACATCTTCTTC+AGG | 0.374474 | 8.1:+22378057 | None:intergenic |
| GGCACCATGAAACTCGGCTT+TGG | 0.378686 | 8.1:+22378138 | None:intergenic |
| TCTTTGAATCTAGCAGCAAT+AGG | 0.389886 | 8.1:+22377652 | None:intergenic |
| GAAACCAAAGCCGAGTTTCA+TGG | 0.392923 | 8.1:-22378142 | MS.gene62904:CDS |
| AAGCTTTGTTCAAGGACGTT+TGG | 0.415966 | 8.1:-22378513 | MS.gene62904:CDS |
| TCCGATGATGAGAAAGAATC+AGG | 0.418255 | 8.1:+22378414 | None:intergenic |
| TAGATCGTTGTTGACGAGTC+GGG | 0.431866 | 8.1:+22378226 | None:intergenic |
| TGATGCTTTCGCGAGACAAA+TGG | 0.443203 | 8.1:-22378029 | MS.gene62904:CDS |
| ACCTGATTCTTTCTCATCAT+CGG | 0.450240 | 8.1:-22378415 | MS.gene62904:CDS |
| TTGTTGTCGTCGTTGTTGTT+TGG | 0.451043 | 8.1:+22378189 | None:intergenic |
| GTGAATTGTGATGGAGTTGT+TGG | 0.452696 | 8.1:+22378117 | None:intergenic |
| GTTTCGATCTTCAAGAGCAT+TGG | 0.459910 | 8.1:-22377875 | MS.gene62904:CDS |
| TTTCGATCTTCAAGAGCATT+GGG | 0.469548 | 8.1:-22377874 | MS.gene62904:CDS |
| TTTGGTTCTGGTCAAGCACT+TGG | 0.469573 | 8.1:-22377754 | MS.gene62904:CDS |
| CTCGTCAACAACGATCTAAA+CGG | 0.483916 | 8.1:-22378221 | MS.gene62904:CDS |
| GGTTCTGGTCAAGCACTTGG+TGG | 0.484093 | 8.1:-22377751 | MS.gene62904:CDS |
| TGAAGAAGAAGAGGGATCGG+TGG | 0.488736 | 8.1:-22377968 | MS.gene62904:CDS |
| TCATCTTCTTCAAGTGGAGC+TGG | 0.500458 | 8.1:+22377607 | None:intergenic |
| ACAAATGCAAGCTTTGTTCA+AGG | 0.502681 | 8.1:-22378521 | MS.gene62904:CDS |
| GCGAAGAATGGTAATGGTAA+TGG | 0.505680 | 8.1:-22377820 | MS.gene62904:CDS |
| ACATAGCAACATCTTCTTCA+GGG | 0.525812 | 8.1:+22378058 | None:intergenic |
| AAGTTATTGAAACGAGTTCG+TGG | 0.531632 | 8.1:-22377928 | MS.gene62904:CDS |
| GCAGTGGAAGTTGATGAAGA+AGG | 0.533864 | 8.1:+22377703 | None:intergenic |
| TGTCGCGATGAAGCGAAGAA+TGG | 0.548591 | 8.1:-22377832 | MS.gene62904:CDS |
| TCTTGTTCAGTGAATTGTGA+TGG | 0.549593 | 8.1:+22378108 | None:intergenic |
| CAGTGGAAGTTGATGAAGAA+GGG | 0.550853 | 8.1:+22377704 | None:intergenic |
| TTTGGCAATGGAAGAGCACT+TGG | 0.550999 | 8.1:-22378495 | MS.gene62904:CDS |
| GTTGTTGGCACCATGAAACT+CGG | 0.554243 | 8.1:+22378132 | None:intergenic |
| TTAGATCGTTGTTGACGAGT+CGG | 0.558622 | 8.1:+22378225 | None:intergenic |
| GGAACAAGATGAAGAAGAAG+AGG | 0.558902 | 8.1:-22377977 | MS.gene62904:CDS |
| AGATCATCATCTTCTTCAAG+TGG | 0.559232 | 8.1:+22377601 | None:intergenic |
| GGCAATGGAAGAGCACTTGG+TGG | 0.563472 | 8.1:-22378492 | MS.gene62904:CDS |
| TGTTCAAGGACGTTTGGCAA+TGG | 0.572618 | 8.1:-22378507 | MS.gene62904:CDS |
| GATGAAGCGAAGAATGGTAA+TGG | 0.574456 | 8.1:-22377826 | MS.gene62904:CDS |
| AGCACTTGGTGGACACATGA+AGG | 0.581864 | 8.1:-22378481 | MS.gene62904:CDS |
| GTTAACATTAACATTAGCAG+TGG | 0.592711 | 8.1:+22377687 | None:intergenic |
| AGATGAAGAAGAAGAGGGAT+CGG | 0.594748 | 8.1:-22377971 | MS.gene62904:CDS |
| AGTTATTGAAACGAGTTCGT+GGG | 0.596995 | 8.1:-22377927 | MS.gene62904:CDS |
| GTATCAGAAACTGAACTCAC+AGG | 0.612015 | 8.1:+22378084 | None:intergenic |
| CATAGCAACATCTTCTTCAG+GGG | 0.618873 | 8.1:+22378059 | None:intergenic |
| GAACAAGATGAAGAAGAAGA+GGG | 0.633530 | 8.1:-22377976 | MS.gene62904:CDS |
| AGCATTTGTGTGAAAACTGT+GGG | 0.639279 | 8.1:-22377903 | MS.gene62904:CDS |
| ATCGGTGGAGAAAATATCGA+AGG | 0.639870 | 8.1:-22377953 | MS.gene62904:CDS |
| GATGAACAACATCAACAATG+TGG | 0.658258 | 8.1:-22377998 | MS.gene62904:CDS |
| AAGCATTTGTGTGAAAACTG+TGG | 0.714401 | 8.1:-22377904 | MS.gene62904:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAATGTCCATTTTGTTTTA+AGG | - | chr8.1:22378331-22378350 | MS.gene62904:CDS | 25.0% |
| CCAAACACCTTAAAACAAAA+TGG | + | chr8.1:22378341-22378360 | None:intergenic | 30.0% | |
| GTTAACATTAACATTAGCAG+TGG | + | chr8.1:22378426-22378445 | None:intergenic | 30.0% | |
| TCACGTACAAAAGAAAATTC+AGG | + | chr8.1:22377810-22377829 | None:intergenic | 30.0% | |
| TCTTCTGTTGTAGAAAATTC+TGG | + | chr8.1:22377950-22377969 | None:intergenic | 30.0% | |
| !!! | CCATTTTGTTTTAAGGTGTT+TGG | - | chr8.1:22378338-22378357 | MS.gene62904:CDS | 30.0% |
| AAGCATTTGTGTGAAAACTG+TGG | - | chr8.1:22378206-22378225 | MS.gene62904:CDS | 35.0% | |
| AAGTTATTGAAACGAGTTCG+TGG | - | chr8.1:22378182-22378201 | MS.gene62904:CDS | 35.0% | |
| ACATAGCAACATCTTCTTCA+GGG | + | chr8.1:22378055-22378074 | None:intergenic | 35.0% | |
| ACCTGATTCTTTCTCATCAT+CGG | - | chr8.1:22377695-22377714 | MS.gene62904:CDS | 35.0% | |
| AGATCATCATCTTCTTCAAG+TGG | + | chr8.1:22378512-22378531 | None:intergenic | 35.0% | |
| AGCATTTGTGTGAAAACTGT+GGG | - | chr8.1:22378207-22378226 | MS.gene62904:CDS | 35.0% | |
| AGTTATTGAAACGAGTTCGT+GGG | - | chr8.1:22378183-22378202 | MS.gene62904:CDS | 35.0% | |
| GAACAAGATGAAGAAGAAGA+GGG | - | chr8.1:22378134-22378153 | MS.gene62904:CDS | 35.0% | |
| GATGAACAACATCAACAATG+TGG | - | chr8.1:22378112-22378131 | MS.gene62904:CDS | 35.0% | |
| TCTTGTTCAGTGAATTGTGA+TGG | + | chr8.1:22378005-22378024 | None:intergenic | 35.0% | |
| TCTTTGAATCTAGCAGCAAT+AGG | + | chr8.1:22378461-22378480 | None:intergenic | 35.0% | |
| !! | ACAAATGCAAGCTTTGTTCA+AGG | - | chr8.1:22377589-22377608 | MS.gene62904:CDS | 35.0% |
| !! | TTTCGATCTTCAAGAGCATT+GGG | - | chr8.1:22378236-22378255 | MS.gene62904:CDS | 35.0% |
| !!! | GCAACTTTGAGACTTTTCTT+GGG | + | chr8.1:22377783-22377802 | None:intergenic | 35.0% |
| !!! | TGCAACTTTGAGACTTTTCT+TGG | + | chr8.1:22377784-22377803 | None:intergenic | 35.0% |
| !!! | TGTTTTAAGGTGTTTGGTTC+TGG | - | chr8.1:22378344-22378363 | MS.gene62904:CDS | 35.0% |
| AGATGAAGAAGAAGAGGGAT+CGG | - | chr8.1:22378139-22378158 | MS.gene62904:CDS | 40.0% | |
| ATCGGTGGAGAAAATATCGA+AGG | - | chr8.1:22378157-22378176 | MS.gene62904:CDS | 40.0% | |
| CACATAGCAACATCTTCTTC+AGG | + | chr8.1:22378056-22378075 | None:intergenic | 40.0% | |
| CATAGCAACATCTTCTTCAG+GGG | + | chr8.1:22378054-22378073 | None:intergenic | 40.0% | |
| CTCGTCAACAACGATCTAAA+CGG | - | chr8.1:22377889-22377908 | MS.gene62904:CDS | 40.0% | |
| GATGAAGCGAAGAATGGTAA+TGG | - | chr8.1:22378284-22378303 | MS.gene62904:CDS | 40.0% | |
| GGAACAAGATGAAGAAGAAG+AGG | - | chr8.1:22378133-22378152 | MS.gene62904:CDS | 40.0% | |
| GTATCAGAAACTGAACTCAC+AGG | + | chr8.1:22378029-22378048 | None:intergenic | 40.0% | |
| TCCGATGATGAGAAAGAATC+AGG | + | chr8.1:22377699-22377718 | None:intergenic | 40.0% | |
| TTAGATCGTTGTTGACGAGT+CGG | + | chr8.1:22377888-22377907 | None:intergenic | 40.0% | |
| ! | GTGAATTGTGATGGAGTTGT+TGG | + | chr8.1:22377996-22378015 | None:intergenic | 40.0% |
| ! | GTTTCGATCTTCAAGAGCAT+TGG | - | chr8.1:22378235-22378254 | MS.gene62904:CDS | 40.0% |
| ! | TTGTTGTCGTCGTTGTTGTT+TGG | + | chr8.1:22377924-22377943 | None:intergenic | 40.0% |
| !! | AAGCTTTGTTCAAGGACGTT+TGG | - | chr8.1:22377597-22377616 | MS.gene62904:CDS | 40.0% |
| !! | CAGTGGAAGTTGATGAAGAA+GGG | + | chr8.1:22378409-22378428 | None:intergenic | 40.0% |
| !! | GCGAAGAATGGTAATGGTAA+TGG | - | chr8.1:22378290-22378309 | MS.gene62904:CDS | 40.0% |
| GAAACCAAAGCCGAGTTTCA+TGG | - | chr8.1:22377968-22377987 | MS.gene62904:CDS | 45.0% | |
| GTTGTTGGCACCATGAAACT+CGG | + | chr8.1:22377981-22378000 | None:intergenic | 45.0% | |
| TAGATCGTTGTTGACGAGTC+GGG | + | chr8.1:22377887-22377906 | None:intergenic | 45.0% | |
| TCATCTTCTTCAAGTGGAGC+TGG | + | chr8.1:22378506-22378525 | None:intergenic | 45.0% | |
| TGATGCTTTCGCGAGACAAA+TGG | - | chr8.1:22378081-22378100 | MS.gene62904:CDS | 45.0% | |
| TGTTCAAGGACGTTTGGCAA+TGG | - | chr8.1:22377603-22377622 | MS.gene62904:CDS | 45.0% | |
| !! | GCAGTGGAAGTTGATGAAGA+AGG | + | chr8.1:22378410-22378429 | None:intergenic | 45.0% |
| !! | TTTGGCAATGGAAGAGCACT+TGG | - | chr8.1:22377615-22377634 | MS.gene62904:CDS | 45.0% |
| !! | TTTGGTTCTGGTCAAGCACT+TGG | - | chr8.1:22378356-22378375 | MS.gene62904:CDS | 45.0% |
| TGAAGAAGAAGAGGGATCGG+TGG | - | chr8.1:22378142-22378161 | MS.gene62904:CDS | 50.0% | |
| TGTCGCGATGAAGCGAAGAA+TGG | - | chr8.1:22378278-22378297 | MS.gene62904:CDS | 50.0% | |
| ! | AGCACTTGGTGGACACATGA+AGG | - | chr8.1:22377629-22377648 | MS.gene62904:CDS | 50.0% |
| GGCACCATGAAACTCGGCTT+TGG | + | chr8.1:22377975-22377994 | None:intergenic | 55.0% | |
| ! | GGTTCTGGTCAAGCACTTGG+TGG | - | chr8.1:22378359-22378378 | MS.gene62904:CDS | 55.0% |
| !! | GGCAATGGAAGAGCACTTGG+TGG | - | chr8.1:22377618-22377637 | MS.gene62904:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 22377579 | 22378553 | 22377579 | ID=MS.gene62904 |
| chr8.1 | mRNA | 22377579 | 22378553 | 22377579 | ID=MS.gene62904.t1;Parent=MS.gene62904 |
| chr8.1 | exon | 22377579 | 22378553 | 22377579 | ID=MS.gene62904.t1.exon1;Parent=MS.gene62904.t1 |
| chr8.1 | CDS | 22377579 | 22378553 | 22377579 | ID=cds.MS.gene62904.t1;Parent=MS.gene62904.t1 |
| Gene Sequence |
| Protein sequence |
