AlfalfaGEDB | MS.gene63897

M. sativa cultivar XinJiangDaYe / MS.gene63897

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63897.t1	XP_024633687.1	56.5	92	39	1	80	171	6	96	3.90E-17	98.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63897.t1	A0A2Z6NBL0	41.9	136	69	5	41	171	4	134	6.5e-14	87.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene63897	0.809787	1.59E-50	-1.69E-46
MS.gene051613	MS.gene63897	0.802543	5.34E-49	-1.69E-46
MS.gene052239	MS.gene63897	-0.812789	3.55E-51	-1.69E-46
MS.gene052447	MS.gene63897	0.87228	3.59E-67	-1.69E-46
MS.gene053082	MS.gene63897	0.809403	1.92E-50	-1.69E-46
MS.gene05329	MS.gene63897	0.846058	2.76E-59	-1.69E-46
MS.gene05415	MS.gene63897	0.843594	1.27E-58	-1.69E-46
MS.gene05531	MS.gene63897	0.813052	3.11E-51	-1.69E-46
MS.gene056174	MS.gene63897	0.831193	1.92E-55	-1.69E-46
MS.gene056272	MS.gene63897	0.810923	9.05E-51	-1.69E-46
MS.gene058294	MS.gene63897	0.838681	2.49E-57	-1.69E-46
MS.gene059272	MS.gene63897	0.80644	8.22E-50	-1.69E-46
MS.gene059458	MS.gene63897	0.811054	8.48E-51	-1.69E-46
MS.gene059459	MS.gene63897	0.80099	1.11E-48	-1.69E-46
MS.gene059584	MS.gene63897	0.826963	2.03E-54	-1.69E-46
MS.gene060257	MS.gene63897	0.806957	6.39E-50	-1.69E-46
MS.gene060521	MS.gene63897	0.803363	3.61E-49	-1.69E-46
MS.gene060654	MS.gene63897	0.806537	7.84E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63897.t1	MTR_2g093430	43.011	93	46	2	85	171	85	176	4.15e-15	72.4
MS.gene63897.t1	MTR_2g083810	41.758	91	48	2	85	171	80	169	6.22e-14	66.2
MS.gene63897.t1	MTR_2g083810	41.758	91	48	2	85	171	80	169	9.76e-14	67.0
MS.gene63897.t1	MTR_2g103990	37.255	102	55	2	59	152	55	155	5.88e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 45 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTGCCAAAGTGGCAGATT+TGG	0.339409	5.1:-75975903	None:intergenic
GCTGTTTGGGAACTAGTTAA+TGG	0.356582	5.1:+75975980	MS.gene63897:CDS
CAAGATTGAGATGGCTGTTT+GGG	0.360205	5.1:+75975967	MS.gene63897:CDS
ACAAGGGAGATCCTTAAAAT+AGG	0.388384	5.1:-75975520	None:intergenic
CCACAGTGTTTACTAATAGA+TGG	0.393695	5.1:+75976068	MS.gene63897:CDS
CCAAAGTGGCAGATTTGGTT+AGG	0.395318	5.1:-75975898	None:intergenic
TCCTAGCTAGAAAGGTGATA+TGG	0.410532	5.1:-75976012	None:intergenic
TTACACCTGTTCGGAATGTT+CGG	0.424114	5.1:+75975843	MS.gene63897:CDS
GTAAGTCCGCAATAATGTTC+AGG	0.429739	5.1:-75975824	None:intergenic
TTTCTCCTCCTTGGAAAGAT+GGG	0.437360	5.1:-75975553	None:intergenic
TCAAGATTGAGATGGCTGTT+TGG	0.443129	5.1:+75975966	MS.gene63897:CDS
CCTAACCAAATCTGCCACTT+TGG	0.457674	5.1:+75975898	MS.gene63897:CDS
GAATCAAAAGTAGCGATGTT+TGG	0.459781	5.1:+75976113	MS.gene63897:CDS
AAATTCCGAACATTCCGAAC+AGG	0.459927	5.1:-75975848	None:intergenic
CCATCTCAATCTTGACAAAT+CGG	0.460947	5.1:-75975958	None:intergenic
TCCTTAAAATAGGTCACGTT+GGG	0.461926	5.1:-75975510	None:intergenic
AGATGGGAAAGAGGCTTACA+AGG	0.476043	5.1:-75975537	None:intergenic
ATTGCGGACTTACACCTGTT+CGG	0.478097	5.1:+75975834	MS.gene63897:CDS
AGGATCGTCGATCAAACGTT+TGG	0.485490	5.1:-75975801	None:intergenic
ATAGATGGATGAGGAAGGTT+CGG	0.487733	5.1:+75976083	MS.gene63897:CDS
TCAAGATTCCCTCTTCTAGC+AGG	0.492324	5.1:-75975630	None:intergenic
ACCATATCACCTTTCTAGCT+AGG	0.492797	5.1:+75976011	MS.gene63897:CDS
CTAGAAAGGTGATATGGTAA+AGG	0.506004	5.1:-75976006	None:intergenic
GCCTCTTTCCCATCTTTCCA+AGG	0.517434	5.1:+75975545	MS.gene63897:CDS
GAAATTCGCTATTGCCAAAG+TGG	0.531967	5.1:-75975912	None:intergenic
TTTGGTTAGGTTGCGAAACA+TGG	0.536494	5.1:-75975885	None:intergenic
AGCAGCATTCCTAGCTAGAA+AGG	0.537666	5.1:-75976020	None:intergenic
TCCTTGGAAAGATGGGAAAG+AGG	0.547994	5.1:-75975546	None:intergenic
ATGAGGAAGGTTCGGAAAGT+CGG	0.565265	5.1:+75976091	MS.gene63897:CDS
GTTAGGTTGCGAAACATGGA+AGG	0.572622	5.1:-75975881	None:intergenic
ATCCTTAAAATAGGTCACGT+TGG	0.584622	5.1:-75975511	None:intergenic
GAGTTGCCGCCTGCTAGAAG+AGG	0.597451	5.1:+75975621	MS.gene63897:CDS
CGTACATCTATTGCATGTAG+AGG	0.602395	5.1:-75975657	None:intergenic
CCGATTTGTCAAGATTGAGA+TGG	0.607654	5.1:+75975958	MS.gene63897:CDS
AGTTGCCGCCTGCTAGAAGA+GGG	0.618271	5.1:+75975622	MS.gene63897:CDS
AGATTCCCTCTTCTAGCAGG+CGG	0.619170	5.1:-75975627	None:intergenic
TGTTTACTAATAGATGGATG+AGG	0.629998	5.1:+75976074	MS.gene63897:CDS
TACTAATAGATGGATGAGGA+AGG	0.630665	5.1:+75976078	MS.gene63897:CDS
ACATCTATTGCATGTAGAGG+AGG	0.639058	5.1:-75975654	None:intergenic
GGAGGAGAAAACTTACATTG+AGG	0.643064	5.1:+75975566	MS.gene63897:CDS
GATGGGAAAGAGGCTTACAA+GGG	0.645216	5.1:-75975536	None:intergenic
TCTTTCCCATCTTTCCAAGG+AGG	0.649920	5.1:+75975548	MS.gene63897:CDS
AGTCCGCAATAATGTTCAGG+AGG	0.674937	5.1:-75975821	None:intergenic
GATCCTCCTGAACATTATTG+CGG	0.679290	5.1:+75975818	MS.gene63897:CDS
CCATCTATTAGTAAACACTG+TGG	0.792095	5.1:-75976068	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAACCAAAAAAGAAGAAAAA+AGG	-	chr5.1:75975756-75975775	None:intergenic	20.0%
!!!	AAACCTTTTTTCTTCTTTTT+TGG	+	chr5.1:75975750-75975769	MS.gene63897:intron	20.0%
!!!	TTTTTTCTTCTTTTTTGGTT+TGG	+	chr5.1:75975755-75975774	MS.gene63897:intron	20.0%
!	GAATGTTCGGAATTTAAAAA+AGG	+	chr5.1:75975856-75975875	MS.gene63897:CDS	25.0%
	AAAAGGAAAAAGTTAGTCGA+GGG	-	chr5.1:75975700-75975719	None:intergenic	30.0%
	TAAAAGGAAAAAGTTAGTCG+AGG	-	chr5.1:75975701-75975720	None:intergenic	30.0%
	TGTTTACTAATAGATGGATG+AGG	+	chr5.1:75976074-75976093	MS.gene63897:CDS	30.0%
	ACAAGGGAGATCCTTAAAAT+AGG	-	chr5.1:75975523-75975542	None:intergenic	35.0%
	ATCCTTAAAATAGGTCACGT+TGG	-	chr5.1:75975514-75975533	None:intergenic	35.0%
	CCACAGTGTTTACTAATAGA+TGG	+	chr5.1:75976068-75976087	MS.gene63897:CDS	35.0%
	CCATCTATTAGTAAACACTG+TGG	-	chr5.1:75976071-75976090	None:intergenic	35.0%
	CCATCTCAATCTTGACAAAT+CGG	-	chr5.1:75975961-75975980	None:intergenic	35.0%
	CTAGAAAGGTGATATGGTAA+AGG	-	chr5.1:75976009-75976028	None:intergenic	35.0%
	GAACGAACATCGATCATAAA+AGG	-	chr5.1:75975717-75975736	None:intergenic	35.0%
	GAATCAAAAGTAGCGATGTT+TGG	+	chr5.1:75976113-75976132	MS.gene63897:CDS	35.0%
	TACTAATAGATGGATGAGGA+AGG	+	chr5.1:75976078-75976097	MS.gene63897:CDS	35.0%
	TCCTTAAAATAGGTCACGTT+GGG	-	chr5.1:75975513-75975532	None:intergenic	35.0%
!	AATGTAAGTTTTCTCCTCCT+TGG	-	chr5.1:75975565-75975584	None:intergenic	35.0%
	AAATTCCGAACATTCCGAAC+AGG	-	chr5.1:75975851-75975870	None:intergenic	40.0%
	ACATCTATTGCATGTAGAGG+AGG	-	chr5.1:75975657-75975676	None:intergenic	40.0%
	ATAGATGGATGAGGAAGGTT+CGG	+	chr5.1:75976083-75976102	MS.gene63897:CDS	40.0%
	CAAGATTGAGATGGCTGTTT+GGG	+	chr5.1:75975967-75975986	MS.gene63897:CDS	40.0%
	CGTACATCTATTGCATGTAG+AGG	-	chr5.1:75975660-75975679	None:intergenic	40.0%
	GAAATTCGCTATTGCCAAAG+TGG	-	chr5.1:75975915-75975934	None:intergenic	40.0%
	GATCCTCCTGAACATTATTG+CGG	+	chr5.1:75975818-75975837	MS.gene63897:CDS	40.0%
	GCTGTTTGGGAACTAGTTAA+TGG	+	chr5.1:75975980-75975999	MS.gene63897:CDS	40.0%
	GGAGGAGAAAACTTACATTG+AGG	+	chr5.1:75975566-75975585	MS.gene63897:CDS	40.0%
	GTAAGTCCGCAATAATGTTC+AGG	-	chr5.1:75975827-75975846	None:intergenic	40.0%
	TATTGCCAAAGTGGCAGATT+TGG	-	chr5.1:75975906-75975925	None:intergenic	40.0%
	TCAAGATTGAGATGGCTGTT+TGG	+	chr5.1:75975966-75975985	MS.gene63897:CDS	40.0%
	TCCTAGCTAGAAAGGTGATA+TGG	-	chr5.1:75976015-75976034	None:intergenic	40.0%
	TGGCATCAAAAACCTGTTGA+GGG	-	chr5.1:75975784-75975803	None:intergenic	40.0%
	TTACACCTGTTCGGAATGTT+CGG	+	chr5.1:75975843-75975862	MS.gene63897:CDS	40.0%
	TTGGCATCAAAAACCTGTTG+AGG	-	chr5.1:75975785-75975804	None:intergenic	40.0%
	TTTCTCCTCCTTGGAAAGAT+GGG	-	chr5.1:75975556-75975575	None:intergenic	40.0%
!	CCGATTTGTCAAGATTGAGA+TGG	+	chr5.1:75975958-75975977	MS.gene63897:CDS	40.0%
!	TTTGGTTAGGTTGCGAAACA+TGG	-	chr5.1:75975888-75975907	None:intergenic	40.0%
!	TTTTCTCCTCCTTGGAAAGA+TGG	-	chr5.1:75975557-75975576	None:intergenic	40.0%
!!	ACCATATCACCTTTCTAGCT+AGG	+	chr5.1:75976011-75976030	MS.gene63897:CDS	40.0%
	AGATGGGAAAGAGGCTTACA+AGG	-	chr5.1:75975540-75975559	None:intergenic	45.0%
	AGCAGCATTCCTAGCTAGAA+AGG	-	chr5.1:75976023-75976042	None:intergenic	45.0%
	AGGATCGTCGATCAAACGTT+TGG	-	chr5.1:75975804-75975823	None:intergenic	45.0%
	AGTCCGCAATAATGTTCAGG+AGG	-	chr5.1:75975824-75975843	None:intergenic	45.0%
	ATGAGGAAGGTTCGGAAAGT+CGG	+	chr5.1:75976091-75976110	MS.gene63897:CDS	45.0%
	ATTGCGGACTTACACCTGTT+CGG	+	chr5.1:75975834-75975853	MS.gene63897:CDS	45.0%
	GATGGGAAAGAGGCTTACAA+GGG	-	chr5.1:75975539-75975558	None:intergenic	45.0%
	TCAAGATTCCCTCTTCTAGC+AGG	-	chr5.1:75975633-75975652	None:intergenic	45.0%
	TCCTTGGAAAGATGGGAAAG+AGG	-	chr5.1:75975549-75975568	None:intergenic	45.0%
	TCTTTCCCATCTTTCCAAGG+AGG	+	chr5.1:75975548-75975567	MS.gene63897:CDS	45.0%
!	CCAAAGTGGCAGATTTGGTT+AGG	-	chr5.1:75975901-75975920	None:intergenic	45.0%
!	CCTAACCAAATCTGCCACTT+TGG	+	chr5.1:75975898-75975917	MS.gene63897:CDS	45.0%
!	GCCCAACGTGACCTATTTTA+AGG	+	chr5.1:75975509-75975528	MS.gene63897:CDS	45.0%
!	GTTAGGTTGCGAAACATGGA+AGG	-	chr5.1:75975884-75975903	None:intergenic	45.0%
!!	TTGGTTTGGTAACCCTCAAC+AGG	+	chr5.1:75975769-75975788	MS.gene63897:intron	45.0%
	AGATTCCCTCTTCTAGCAGG+CGG	-	chr5.1:75975630-75975649	None:intergenic	50.0%
	GCCTCTTTCCCATCTTTCCA+AGG	+	chr5.1:75975545-75975564	MS.gene63897:CDS	50.0%
	AGTTGCCGCCTGCTAGAAGA+GGG	+	chr5.1:75975622-75975641	MS.gene63897:CDS	55.0%
	GAGTTGCCGCCTGCTAGAAG+AGG	+	chr5.1:75975621-75975640	MS.gene63897:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	75975507	75976138	75975507	ID=MS.gene63897
chr5.1	mRNA	75975507	75976138	75975507	ID=MS.gene63897.t1;Parent=MS.gene63897
chr5.1	exon	75975507	75975674	75975507	ID=MS.gene63897.t1.exon1;Parent=MS.gene63897.t1
chr5.1	CDS	75975507	75975674	75975507	ID=cds.MS.gene63897.t1;Parent=MS.gene63897.t1
chr5.1	exon	75975791	75976138	75975791	ID=MS.gene63897.t1.exon2;Parent=MS.gene63897.t1
chr5.1	CDS	75975791	75976138	75975791	ID=cds.MS.gene63897.t1;Parent=MS.gene63897.t1

Gene Sequence

>MS.gene63897

ATGCCCAACGTGACCTATTTTAAGGATCTCCCTTGTAAGCCTCTTTCCCATCTTTCCAAGGAGGAGAAAACTTACATTGAGGAATCGCTTGTTTGTGCTGCTGATCTTGAAGAAGAGTTGCCGCCTGCTAGAAGAGGGAATCTTGAACCTCCTCTACATGCAATAGATGTACGTACTTGAAATTTCATATCCCTCGACTAACTTTTTCCTTTTATGATCGATGTTCGTTCATTTTACAATCGTAAACCTTTTTTCTTCTTTTTTGGTTTGGTAACCCTCAACAGGTTTTTGATGCCAAACGTTTGATCGACGATCCTCCTGAACATTATTGCGGACTTACACCTGTTCGGAATGTTCGGAATTTAAAAAAGGATCCTTCCATGTTTCGCAACCTAACCAAATCTGCCACTTTGGCAATAGCGAATTTCAATTCAATGAAAAAGACAAACTACCGATTTGTCAAGATTGAGATGGCTGTTTGGGAACTAGTTAATGGAGTCCTTTACCATATCACCTTTCTAGCTAGGAATGCTGCTGAGAAGAAAAATGAAACTTTTGAAGCCACAGTGTTTACTAATAGATGGATGAGGAAGGTTCGGAAAGTCGGAATCAAAAGTAGCGATGTTTGGTAA

Protein sequence

>MS.gene63897.t1

MPNVTYFKDLPCKPLSHLSKEEKTYIEESLVCAADLEEELPPARRGNLEPPLHAIDVFDAKRLIDDPPEHYCGLTPVRNVRNLKKDPSMFRNLTKSATLAIANFNSMKKTNYRFVKIEMAVWELVNGVLYHITFLARNAAEKKNETFEATVFTNRWMRKVRKVGIKSSDVW