Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64344.t1 | XP_003603759.1 | 94.2 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 3.00E-49 | 204.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64344.t1 | P46689 | 58.3 | 103 | 38 | 1 | 1 | 103 | 1 | 98 | 9.6e-28 | 124.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64344.t1 | G7J582 | 94.2 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 2.2e-49 | 204.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene64344 | 0.827056 | 1.93E-54 | -1.69E-46 |
| MS.gene050170 | MS.gene64344 | 0.835192 | 1.94E-56 | -1.69E-46 |
| MS.gene050323 | MS.gene64344 | 0.816827 | 4.52E-52 | -1.69E-46 |
| MS.gene050496 | MS.gene64344 | 0.843458 | 1.38E-58 | -1.69E-46 |
| MS.gene050697 | MS.gene64344 | 0.803107 | 4.08E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene64344 | 0.834957 | 2.22E-56 | -1.69E-46 |
| MS.gene050871 | MS.gene64344 | 0.838936 | 2.14E-57 | -1.69E-46 |
| MS.gene051268 | MS.gene64344 | 0.804321 | 2.29E-49 | -1.69E-46 |
| MS.gene052066 | MS.gene64344 | 0.813714 | 2.23E-51 | -1.69E-46 |
| MS.gene052482 | MS.gene64344 | 0.810045 | 1.40E-50 | -1.69E-46 |
| MS.gene053633 | MS.gene64344 | 0.800373 | 1.49E-48 | -1.69E-46 |
| MS.gene05589 | MS.gene64344 | 0.814446 | 1.53E-51 | -1.69E-46 |
| MS.gene056310 | MS.gene64344 | -0.811048 | 8.51E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene64344 | 0.839597 | 1.44E-57 | -1.69E-46 |
| MS.gene056878 | MS.gene64344 | 0.80049 | 1.41E-48 | -1.69E-46 |
| MS.gene058157 | MS.gene64344 | 0.813429 | 2.57E-51 | -1.69E-46 |
| MS.gene058171 | MS.gene64344 | -0.823768 | 1.16E-53 | -1.69E-46 |
| MS.gene058173 | MS.gene64344 | -0.828736 | 7.62E-55 | -1.69E-46 |
| MS.gene058175 | MS.gene64344 | -0.824003 | 1.02E-53 | -1.69E-46 |
| MS.gene059045 | MS.gene64344 | -0.812466 | 4.18E-51 | -1.69E-46 |
| MS.gene059837 | MS.gene64344 | 0.806046 | 9.95E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene64344 | 0.825147 | 5.49E-54 | -1.69E-46 |
| MS.gene06000 | MS.gene64344 | 0.838705 | 2.45E-57 | -1.69E-46 |
| MS.gene060187 | MS.gene64344 | -0.816734 | 4.75E-52 | -1.69E-46 |
| MS.gene060595 | MS.gene64344 | -0.853224 | 2.75E-61 | -1.69E-46 |
| MS.gene060778 | MS.gene64344 | 0.863416 | 2.53E-64 | -1.69E-46 |
| MS.gene06077 | MS.gene64344 | 0.809349 | 1.98E-50 | -1.69E-46 |
| MS.gene060982 | MS.gene64344 | 0.809769 | 1.61E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64344.t1 | MTR_3g113890 | 94.175 | 103 | 6 | 0 | 1 | 103 | 1 | 103 | 5.10e-68 | 199 |
| MS.gene64344.t1 | MTR_1g025220 | 66.990 | 103 | 32 | 1 | 1 | 103 | 1 | 101 | 1.17e-45 | 142 |
| MS.gene64344.t1 | MTR_1g025250 | 66.990 | 103 | 31 | 1 | 1 | 103 | 1 | 100 | 7.06e-38 | 123 |
| MS.gene64344.t1 | MTR_1g096370 | 52.222 | 90 | 42 | 1 | 14 | 103 | 7 | 95 | 1.79e-28 | 99.4 |
| MS.gene64344.t1 | MTR_4g106860 | 52.381 | 84 | 37 | 2 | 21 | 103 | 34 | 115 | 2.68e-24 | 89.4 |
| MS.gene64344.t1 | MTR_8g075920 | 52.326 | 86 | 40 | 1 | 18 | 103 | 19 | 103 | 4.52e-23 | 85.9 |
| MS.gene64344.t1 | MTR_3g113850 | 88.889 | 45 | 5 | 0 | 44 | 88 | 406 | 450 | 6.91e-21 | 85.9 |
| MS.gene64344.t1 | MTR_8g062800 | 39.815 | 108 | 53 | 2 | 8 | 103 | 5 | 112 | 1.25e-20 | 79.7 |
| MS.gene64344.t1 | MTR_6g007897 | 57.377 | 61 | 25 | 1 | 44 | 103 | 188 | 248 | 1.12e-15 | 70.1 |
| MS.gene64344.t1 | MTR_5g036880 | 36.275 | 102 | 49 | 2 | 2 | 103 | 7 | 92 | 5.23e-15 | 65.1 |
| MS.gene64344.t1 | MTR_3g102660 | 49.153 | 59 | 30 | 0 | 45 | 103 | 27 | 85 | 7.98e-15 | 64.3 |
| MS.gene64344.t1 | MTR_5g036720 | 36.458 | 96 | 57 | 2 | 8 | 103 | 1 | 92 | 8.80e-15 | 64.3 |
| MS.gene64344.t1 | MTR_7g090590 | 33.884 | 121 | 61 | 3 | 1 | 103 | 1 | 120 | 5.39e-13 | 60.5 |
| MS.gene64344.t1 | MTR_1g106915 | 35.714 | 70 | 45 | 0 | 34 | 103 | 21 | 90 | 9.31e-13 | 58.9 |
| MS.gene64344.t1 | MTR_6g012360 | 32.710 | 107 | 66 | 2 | 3 | 103 | 1 | 107 | 2.60e-12 | 58.5 |
| MS.gene64344.t1 | MTR_7g010580 | 31.731 | 104 | 68 | 1 | 3 | 103 | 6 | 109 | 2.63e-12 | 58.5 |
| MS.gene64344.t1 | MTR_5g036860 | 33.333 | 96 | 60 | 2 | 8 | 103 | 1 | 92 | 3.66e-12 | 57.8 |
| MS.gene64344.t1 | MTR_5g095990 | 40.260 | 77 | 44 | 2 | 27 | 103 | 75 | 149 | 4.20e-12 | 58.9 |
| MS.gene64344.t1 | MTR_5g036640 | 40.580 | 69 | 41 | 0 | 35 | 103 | 15 | 83 | 9.11e-12 | 56.2 |
| MS.gene64344.t1 | MTR_3g037750 | 34.694 | 98 | 63 | 1 | 6 | 103 | 7 | 103 | 1.80e-11 | 56.2 |
| MS.gene64344.t1 | MTR_3g007900 | 45.000 | 60 | 33 | 0 | 44 | 103 | 95 | 154 | 2.36e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64344.t1 | AT1G75750 | 68.056 | 72 | 23 | 0 | 32 | 103 | 27 | 98 | 6.36e-32 | 108 |
| MS.gene64344.t1 | AT1G75750 | 68.056 | 72 | 23 | 0 | 32 | 103 | 26 | 97 | 6.45e-32 | 107 |
| MS.gene64344.t1 | AT2G18420 | 68.919 | 74 | 22 | 1 | 31 | 103 | 21 | 94 | 2.70e-30 | 103 |
| MS.gene64344.t1 | AT2G18420 | 75.806 | 62 | 15 | 0 | 42 | 103 | 9 | 70 | 4.55e-29 | 100 |
| MS.gene64344.t1 | AT4G09600 | 59.494 | 79 | 29 | 1 | 28 | 103 | 21 | 99 | 2.03e-26 | 94.0 |
| MS.gene64344.t1 | AT1G22690 | 61.290 | 62 | 24 | 0 | 42 | 103 | 58 | 119 | 5.14e-23 | 86.3 |
| MS.gene64344.t1 | AT1G22690 | 61.290 | 62 | 24 | 0 | 42 | 103 | 58 | 119 | 5.14e-23 | 86.3 |
| MS.gene64344.t1 | AT1G22690 | 61.290 | 62 | 24 | 0 | 42 | 103 | 58 | 119 | 5.14e-23 | 86.3 |
| MS.gene64344.t1 | AT4G09610 | 62.025 | 79 | 27 | 1 | 28 | 103 | 21 | 99 | 1.62e-19 | 76.6 |
| MS.gene64344.t1 | AT5G14920 | 56.923 | 65 | 27 | 1 | 40 | 103 | 211 | 275 | 4.48e-17 | 74.3 |
| MS.gene64344.t1 | AT1G74670 | 36.735 | 98 | 61 | 1 | 7 | 103 | 4 | 101 | 1.10e-15 | 67.0 |
| MS.gene64344.t1 | AT5G59845 | 36.000 | 100 | 46 | 2 | 5 | 103 | 7 | 89 | 1.83e-14 | 63.5 |
| MS.gene64344.t1 | AT1G10588 | 45.000 | 60 | 32 | 1 | 45 | 103 | 31 | 90 | 1.87e-12 | 58.5 |
| MS.gene64344.t1 | AT1G10588 | 34.043 | 94 | 52 | 2 | 11 | 103 | 5 | 89 | 1.90e-12 | 58.2 |
| MS.gene64344.t1 | AT2G39540 | 32.258 | 93 | 54 | 1 | 11 | 103 | 4 | 87 | 4.18e-12 | 57.4 |
| MS.gene64344.t1 | AT5G15230 | 29.907 | 107 | 58 | 2 | 6 | 103 | 8 | 106 | 3.70e-11 | 55.5 |
Find 0 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATATATGAACTAAACTA+AGG | - | 24038:9411-9430 | MS.gene64344:intron | 15.0% |
| !! | TGGAAAAAATATAAAAAAAC+TGG | - | 24038:9365-9384 | MS.gene64344:intron | 15.0% |
| !!! | TTTTAATTATTTGCTTAAAG+TGG | - | 24038:9345-9364 | MS.gene64344:intron | 15.0% |
| !! | ATATAAATTTCAGATTGCAA+TGG | - | 24038:9685-9704 | MS.gene64344:intron | 20.0% |
| !! | GGATAAAAGATTAGTAAAAA+AGG | - | 24038:9525-9544 | MS.gene64344:intron | 20.0% |
| !!! | TATTTTATAAAGAAAACGCT+TGG | + | 24038:9203-9222 | MS.gene64344:intergenic | 20.0% |
| ! | TAACGAATATGTATGCTAAT+GGG | - | 24038:9504-9523 | MS.gene64344:intron | 25.0% |
| ! | TTAACGAATATGTATGCTAA+TGG | - | 24038:9503-9522 | MS.gene64344:intron | 25.0% |
| !! | TTAATTACATGTGTTTTCGT+AGG | - | 24038:9239-9258 | MS.gene64344:intron | 25.0% |
| ! | CTACTTCACACACATATTTT+TGG | + | 24038:9653-9672 | MS.gene64344:intergenic | 30.0% |
| !! | CAACAATAGCTTTGAATTAC+CGG | + | 24038:9150-9169 | MS.gene64344:intergenic | 30.0% |
| AGCATGAACAAGATGGAGAA+TGG | + | 24038:9118-9137 | MS.gene64344:intergenic | 40.0% | |
| TAAAGAAAACGCTTGGAGCT+TGG | + | 24038:9196-9215 | MS.gene64344:intergenic | 40.0% | |
| TCTCCATCTTGTTCATGCTT+TGG | - | 24038:9119-9138 | MS.gene64344:CDS | 40.0% | |
| ! | GTTTCTTTTCAGCAGCAGAT+AGG | - | 24038:9288-9307 | MS.gene64344:intron | 40.0% |
| !! | AATAGCTTTGAATTACCGGC+TGG | + | 24038:9146-9165 | MS.gene64344:intergenic | 40.0% |
| AACCAGACAGAAGCAGTTGA+TGG | + | 24038:9327-9346 | MS.gene64343:intergenic | 45.0% | |
| CATAGCAGGGACACATTTCT+TGG | + | 24038:9819-9838 | MS.gene64344:intergenic | 45.0% | |
| GGTCCAAAGCATGAACAAGA+TGG | + | 24038:9125-9144 | MS.gene64344:intergenic | 45.0% | |
| TCTTGGTTACCAGAAGTTCC+TGG | + | 24038:9802-9821 | MS.gene64344:intergenic | 45.0% | |
| TGAGTTTCTGCGTGTACATG+CGG | + | 24038:9267-9286 | MS.gene64344:intergenic | 45.0% | |
| TTCCATCAACTGCTTCTGTC+TGG | - | 24038:9322-9341 | MS.gene64344:intron | 45.0% | |
| !! | CTAGGTGCCGTTTATCATCA+CGG | - | 24038:9719-9738 | MS.gene64344:intron | 45.0% |
| !! | GTAATTAAATTAAATTAAAT+TGG | + | 24038:9227-9246 | MS.gene64344:intergenic | 5.0% |
| TATGCCAACCAAACCACCCA+TGG | - | 24038:9835-9854 | MS.gene64344:CDS | 50.0% | |
| TGGTTACCAGAAGTTCCTGG+TGG | + | 24038:9799-9818 | MS.gene64344:intergenic | 50.0% | |
| ! | TGTTCATGCTTTGGACCAGC+CGG | - | 24038:9128-9147 | MS.gene64344:CDS | 50.0% |
| !! | CATGTGGAACTTGCTGCAGT+CGG | - | 24038:9761-9780 | MS.gene64344:intron | 50.0% |
| !! | GTGGTTTGGTTGGCATAGCA+GGG | + | 24038:9832-9851 | MS.gene64344:intergenic | 50.0% |
| AGCAAGTTCCACATGCCCTG+TGG | + | 24038:9756-9775 | MS.gene64344:intergenic | 55.0% | |
| ! | GCAATGGAGCATGTGCTGCT+AGG | - | 24038:9701-9720 | MS.gene64344:intron | 55.0% |
| !! | GGTGGTTTGGTTGGCATAGC+AGG | + | 24038:9833-9852 | MS.gene64344:intergenic | 55.0% |
| GCACTTGCGTCTGCCTCCAT+GGG | + | 24038:9854-9873 | MS.gene64344:intergenic | 60.0% | |
| GCCAACCAAACCACCCATGG+AGG | - | 24038:9838-9857 | MS.gene64344:CDS | 60.0% | |
| GGCGCGGCCGTGATGATAAA+CGG | + | 24038:9729-9748 | MS.gene64344:intergenic | 60.0% | |
| GTCTGCCTCCATGGGTGGTT+TGG | + | 24038:9846-9865 | MS.gene64344:intergenic | 60.0% | |
| TGCGTGCCACCAGGAACTTC+TGG | - | 24038:9790-9809 | MS.gene64344:intron | 60.0% | |
| TTCCACATGCCCTGTGGCAG+AGG | + | 24038:9750-9769 | MS.gene64344:intergenic | 60.0% | |
| !! | GCCTCCATGGGTGGTTTGGT+TGG | + | 24038:9842-9861 | MS.gene64344:intergenic | 60.0% |
| CTTGCGTCTGCCTCCATGGG+TGG | + | 24038:9851-9870 | MS.gene64344:intergenic | 65.0% | |
| GGCACTTGCGTCTGCCTCCA+TGG | + | 24038:9855-9874 | MS.gene64344:intergenic | 65.0% | |
| CATGCCCTGTGGCAGAGGCG+CGG | + | 24038:9745-9764 | MS.gene64344:intergenic | 70.0% | |
| CGCCTCTGCCACAGGGCATG+TGG | - | 24038:9745-9764 | MS.gene64344:intron | 70.0% | |
| CGGTGCAACTGCGTGCCACC+AGG | - | 24038:9781-9800 | MS.gene64344:intron | 70.0% | |
| ACGGCCGCGCCTCTGCCACA+GGG | - | 24038:9738-9757 | MS.gene64344:intron | 75.0% | |
| ! | CACGGCCGCGCCTCTGCCAC+AGG | - | 24038:9737-9756 | MS.gene64344:intron | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 24038 | gene | 9069 | 9879 | 9069 | ID=MS.gene64344 |
| 24038 | mRNA | 9069 | 9879 | 9069 | ID=MS.gene64344.t1;Parent=MS.gene64344 |
| 24038 | exon | 9799 | 9879 | 9799 | ID=MS.gene64344.t1.exon1;Parent=MS.gene64344.t1 |
| 24038 | CDS | 9799 | 9879 | 9799 | ID=cds.MS.gene64344.t1;Parent=MS.gene64344.t1 |
| 24038 | exon | 9639 | 9687 | 9639 | ID=MS.gene64344.t1.exon2;Parent=MS.gene64344.t1 |
| 24038 | CDS | 9639 | 9687 | 9639 | ID=cds.MS.gene64344.t1;Parent=MS.gene64344.t1 |
| 24038 | exon | 9069 | 9250 | 9069 | ID=MS.gene64344.t1.exon3;Parent=MS.gene64344.t1 |
| 24038 | CDS | 9069 | 9250 | 9069 | ID=cds.MS.gene64344.t1;Parent=MS.gene64344.t1 |
| Gene Sequence |
| Protein sequence |
