Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64636.t1 | XP_013459438.1 | 98.7 | 301 | 4 | 0 | 1 | 301 | 1 | 301 | 4.90E-148 | 533.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64636.t1 | Q9FJD0 | 78.7 | 301 | 64 | 0 | 1 | 301 | 1 | 301 | 2.2e-120 | 433.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64636.t1 | A0A072UUG1 | 98.7 | 301 | 4 | 0 | 1 | 301 | 1 | 301 | 3.5e-148 | 533.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049669 | MS.gene64636 | 0.811928 | 5.48E-51 | -1.69E-46 |
| MS.gene050442 | MS.gene64636 | 0.821575 | 3.75E-53 | -1.69E-46 |
| MS.gene050719 | MS.gene64636 | 0.801649 | 8.15E-49 | -1.69E-46 |
| MS.gene050721 | MS.gene64636 | 0.801458 | 8.92E-49 | -1.69E-46 |
| MS.gene050787 | MS.gene64636 | 0.822068 | 2.89E-53 | -1.69E-46 |
| MS.gene051265 | MS.gene64636 | 0.801927 | 7.15E-49 | -1.69E-46 |
| MS.gene052333 | MS.gene64636 | 0.804766 | 1.84E-49 | -1.69E-46 |
| MS.gene052586 | MS.gene64636 | 0.805711 | 1.17E-49 | -1.69E-46 |
| MS.gene053147 | MS.gene64636 | 0.81963 | 1.05E-52 | -1.69E-46 |
| MS.gene05414 | MS.gene64636 | 0.806885 | 6.62E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene64636 | 0.809553 | 1.79E-50 | -1.69E-46 |
| MS.gene054291 | MS.gene64636 | -0.804786 | 1.83E-49 | -1.69E-46 |
| MS.gene054467 | MS.gene64636 | 0.829331 | 5.47E-55 | -1.69E-46 |
| MS.gene054468 | MS.gene64636 | 0.845254 | 4.56E-59 | -1.69E-46 |
| MS.gene054581 | MS.gene64636 | 0.811038 | 8.55E-51 | -1.69E-46 |
| MS.gene055356 | MS.gene64636 | 0.825946 | 3.55E-54 | -1.69E-46 |
| MS.gene055357 | MS.gene64636 | 0.815925 | 7.20E-52 | -1.69E-46 |
| MS.gene055358 | MS.gene64636 | 0.829012 | 6.53E-55 | -1.69E-46 |
| MS.gene05604 | MS.gene64636 | 0.810072 | 1.38E-50 | -1.69E-46 |
| MS.gene057026 | MS.gene64636 | 0.843117 | 1.71E-58 | -1.69E-46 |
| MS.gene057414 | MS.gene64636 | 0.80036 | 1.50E-48 | -1.69E-46 |
| MS.gene059272 | MS.gene64636 | 0.811751 | 5.98E-51 | -1.69E-46 |
| MS.gene059584 | MS.gene64636 | 0.807853 | 4.13E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene64636 | 0.833624 | 4.79E-56 | -1.69E-46 |
| MS.gene06052 | MS.gene64636 | 0.806627 | 7.50E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene64636 | -0.814617 | 1.41E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64636.t1 | MTR_3g040070 | 98.671 | 301 | 4 | 0 | 1 | 301 | 1 | 301 | 0.0 | 603 |
| MS.gene64636.t1 | MTR_8g015430 | 79.734 | 301 | 61 | 0 | 1 | 301 | 1 | 301 | 0.0 | 505 |
| MS.gene64636.t1 | MTR_3g040070 | 97.551 | 245 | 6 | 0 | 1 | 245 | 1 | 245 | 8.35e-176 | 486 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene64636.t1 | AT5G53530 | 77.409 | 301 | 68 | 0 | 1 | 301 | 1 | 301 | 2.18e-178 | 494 |
| MS.gene64636.t1 | AT4G27690 | 77.409 | 301 | 68 | 0 | 1 | 301 | 1 | 301 | 6.05e-178 | 493 |
| MS.gene64636.t1 | AT4G27690 | 79.853 | 273 | 55 | 0 | 29 | 301 | 1 | 273 | 3.95e-166 | 462 |
Find 60 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGCAAAATTTCAATCTCTT+TGG | 0.260579 | 3.3:-30231548 | MS.gene64636:CDS |
| ATTGAGCTTCTTGGTCAAAT+AGG | 0.268030 | 3.3:-30229908 | MS.gene64636:intron |
| CCTTGCGAAGTTTGAGTTGA+TGG | 0.292292 | 3.3:-30223680 | MS.gene64636:CDS |
| TTCCTTTCATATAAATTTCC+AGG | 0.334470 | 3.3:+30229177 | None:intergenic |
| CCTAACATTGATCCCATTGT+AGG | 0.348421 | 3.3:+30229110 | None:intergenic |
| TTCTATGACTTTACTTCTCT+TGG | 0.354378 | 3.3:-30229531 | MS.gene64636:intron |
| ATCGATTAATAACAGCATTA+AGG | 0.356615 | 3.3:-30224342 | MS.gene64636:intron |
| CTTACCTGCTTGCGAGTTGT+TGG | 0.372393 | 3.3:+30231520 | None:intergenic |
| CGTCGAGAGTCAACGGGATC+AGG | 0.380725 | 3.3:-30223730 | MS.gene64636:CDS |
| CAAAGTCAGGAGAATATAGT+TGG | 0.385504 | 3.3:-30230094 | MS.gene64636:CDS |
| GGTTCCTCTCTTTCAAAGTC+AGG | 0.403485 | 3.3:-30230107 | MS.gene64636:CDS |
| TGATTGCAGAATATAATTGC+TGG | 0.406483 | 3.3:-30231586 | MS.gene64636:intron |
| CTCCGTTTCAACATATGTAT+TGG | 0.428647 | 3.3:+30223705 | None:intergenic |
| CCTACAATGGGATCAATGTT+AGG | 0.428965 | 3.3:-30229110 | MS.gene64636:CDS |
| TCAACAGTGGAGAACTCAAA+TGG | 0.438119 | 3.3:+30229147 | None:intergenic |
| GGAAAATGACCAGACGGTTA+TGG | 0.444084 | 3.3:-30230128 | MS.gene64636:CDS |
| TATGTTGGTGTCAATTCATA+TGG | 0.444837 | 3.3:+30222524 | None:intergenic |
| AAAACATGGAACTTGAAATC+AGG | 0.446337 | 3.3:-30223753 | MS.gene64636:CDS |
| GTACGCGAACTTGATGTTCC+TGG | 0.461881 | 3.3:-30229195 | MS.gene64636:CDS |
| GGGAAGAAGGTTGAGCATAA+TGG | 0.464127 | 3.3:-30229938 | MS.gene64636:CDS |
| ATGGGATCAATGTTAGGCTT+AGG | 0.482168 | 3.3:-30229104 | MS.gene64636:intron |
| TTTATAGGTTCGCGTGAAGA+AGG | 0.483598 | 3.3:-30230149 | MS.gene64636:intron |
| TTCCTGGAAATTTATATGAA+AGG | 0.485870 | 3.3:-30229179 | MS.gene64636:CDS |
| GTGTCAATTCATATGGATTG+AGG | 0.487376 | 3.3:+30222531 | None:intergenic |
| AAAGTCAGGAGAATATAGTT+GGG | 0.487916 | 3.3:-30230093 | MS.gene64636:CDS |
| CATCTGAAGGATGTTGTCAT+TGG | 0.491672 | 3.3:-30223811 | MS.gene64636:CDS |
| TTGTTGATATTGTGATATGT+TGG | 0.494473 | 3.3:+30222509 | None:intergenic |
| GACCAATACATATGTTGAAA+CGG | 0.497385 | 3.3:-30223707 | MS.gene64636:CDS |
| TCAACCTTCTTCCCACGTAC+AGG | 0.501860 | 3.3:+30229947 | None:intergenic |
| TGCCGCATGAATCCTACAAT+GGG | 0.507154 | 3.3:-30229122 | MS.gene64636:CDS |
| ATCAGGCGTCGAGAGTCAAC+GGG | 0.511333 | 3.3:-30223736 | MS.gene64636:CDS |
| CAACAGTGGAGAACTCAAAT+GGG | 0.517262 | 3.3:+30229148 | None:intergenic |
| CAACAACATTGTGGAACACA+TGG | 0.518191 | 3.3:-30227950 | MS.gene64636:CDS |
| AATCAGGCGTCGAGAGTCAA+CGG | 0.523164 | 3.3:-30223737 | MS.gene64636:CDS |
| CCATCAACTCAAACTTCGCA+AGG | 0.549987 | 3.3:+30223680 | None:intergenic |
| AGAACCTGTACGTGGGAAGA+AGG | 0.550419 | 3.3:-30229951 | MS.gene64636:CDS |
| AGAGAGGAACCATAACCGTC+TGG | 0.552534 | 3.3:+30230119 | None:intergenic |
| GTCGAGAGTCAACGGGATCA+GGG | 0.552801 | 3.3:-30223729 | MS.gene64636:CDS |
| CTTGCTGCTTGAAGTAGCGC+CGG | 0.553824 | 3.3:+30222435 | None:intergenic |
| TCATCCAACAACTCGCAAGC+AGG | 0.555468 | 3.3:-30231524 | MS.gene64636:intron |
| CTGTTATTAATCGATGGTGC+TGG | 0.557386 | 3.3:+30224350 | None:intergenic |
| TTAATGCTGTTATTAATCGA+TGG | 0.557771 | 3.3:+30224344 | None:intergenic |
| TTCTCCTGACTTTGAAAGAG+AGG | 0.557843 | 3.3:+30230103 | None:intergenic |
| TAAATGCCGCAGATGGAAGT+AGG | 0.565922 | 3.3:-30223974 | MS.gene64636:intron |
| ATGGATGGTGTTCCAGTCCG+AGG | 0.573375 | 3.3:-30223661 | MS.gene64636:intron |
| GGATTGAGGAATAATCTGAC+AGG | 0.584581 | 3.3:+30222545 | None:intergenic |
| AGTAACTACAAACCTCGGAC+TGG | 0.584953 | 3.3:+30223649 | None:intergenic |
| GCGAAGTTTGAGTTGATGGA+TGG | 0.592194 | 3.3:-30223676 | MS.gene64636:CDS |
| ATGCCGCATGAATCCTACAA+TGG | 0.593119 | 3.3:-30229123 | MS.gene64636:CDS |
| AATTAACAGGTATCATCTGA+AGG | 0.593587 | 3.3:-30223824 | MS.gene64636:intron |
| GCAGAATTGTACTTTGACAG+AGG | 0.603352 | 3.3:-30229558 | MS.gene64636:intron |
| TTCATGCGGCATTTCAACAG+TGG | 0.609425 | 3.3:+30229134 | None:intergenic |
| TCAGGAGAATATAGTTGGGG+AGG | 0.630279 | 3.3:-30230089 | MS.gene64636:intron |
| ATCCCATTGTAGGATTCATG+CGG | 0.642487 | 3.3:+30229120 | None:intergenic |
| CATCAACTCAAACTTCGCAA+GGG | 0.643439 | 3.3:+30223681 | None:intergenic |
| GTTAGTATAGAACCTGTACG+TGG | 0.648956 | 3.3:-30229959 | MS.gene64636:CDS |
| AAGTCAGGAGAATATAGTTG+GGG | 0.654882 | 3.3:-30230092 | MS.gene64636:CDS |
| GAAGAAGGAAAATGACCAGA+CGG | 0.657428 | 3.3:-30230134 | MS.gene64636:CDS |
| ATATTCAGGAGTCTTCAAGT+AGG | 0.675141 | 3.3:+30222396 | None:intergenic |
| TTAGTATAGAACCTGTACGT+GGG | 0.696791 | 3.3:-30229958 | MS.gene64636:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 30222400 | 30231676 | 30222400 | ID=MS.gene64636 |
| chr3.3 | mRNA | 30222400 | 30231676 | 30222400 | ID=MS.gene64636.t1;Parent=MS.gene64636 |
| chr3.3 | exon | 30231674 | 30231676 | 30231674 | ID=MS.gene64636.t1.exon1;Parent=MS.gene64636.t1 |
| chr3.3 | CDS | 30231674 | 30231676 | 30231674 | ID=cds.MS.gene64636.t1;Parent=MS.gene64636.t1 |
| chr3.3 | exon | 30231525 | 30231599 | 30231525 | ID=MS.gene64636.t1.exon2;Parent=MS.gene64636.t1 |
| chr3.3 | CDS | 30231525 | 30231599 | 30231525 | ID=cds.MS.gene64636.t1;Parent=MS.gene64636.t1 |
| chr3.3 | exon | 30230090 | 30230164 | 30230090 | ID=MS.gene64636.t1.exon3;Parent=MS.gene64636.t1 |
| chr3.3 | CDS | 30230090 | 30230164 | 30230090 | ID=cds.MS.gene64636.t1;Parent=MS.gene64636.t1 |
| chr3.3 | exon | 30229909 | 30229981 | 30229909 | ID=MS.gene64636.t1.exon4;Parent=MS.gene64636.t1 |
| chr3.3 | CDS | 30229909 | 30229981 | 30229909 | ID=cds.MS.gene64636.t1;Parent=MS.gene64636.t1 |
| chr3.3 | exon | 30229532 | 30229576 | 30229532 | ID=MS.gene64636.t1.exon5;Parent=MS.gene64636.t1 |
| chr3.3 | CDS | 30229532 | 30229576 | 30229532 | ID=cds.MS.gene64636.t1;Parent=MS.gene64636.t1 |
| chr3.3 | exon | 30229105 | 30229216 | 30229105 | ID=MS.gene64636.t1.exon6;Parent=MS.gene64636.t1 |
| chr3.3 | CDS | 30229105 | 30229216 | 30229105 | ID=cds.MS.gene64636.t1;Parent=MS.gene64636.t1 |
| chr3.3 | exon | 30227942 | 30228008 | 30227942 | ID=MS.gene64636.t1.exon7;Parent=MS.gene64636.t1 |
| chr3.3 | CDS | 30227942 | 30228008 | 30227942 | ID=cds.MS.gene64636.t1;Parent=MS.gene64636.t1 |
| chr3.3 | exon | 30224343 | 30224387 | 30224343 | ID=MS.gene64636.t1.exon8;Parent=MS.gene64636.t1 |
| chr3.3 | CDS | 30224343 | 30224387 | 30224343 | ID=cds.MS.gene64636.t1;Parent=MS.gene64636.t1 |
| chr3.3 | exon | 30223929 | 30223984 | 30223929 | ID=MS.gene64636.t1.exon9;Parent=MS.gene64636.t1 |
| chr3.3 | CDS | 30223929 | 30223984 | 30223929 | ID=cds.MS.gene64636.t1;Parent=MS.gene64636.t1 |
| chr3.3 | exon | 30223662 | 30223837 | 30223662 | ID=MS.gene64636.t1.exon10;Parent=MS.gene64636.t1 |
| chr3.3 | CDS | 30223662 | 30223837 | 30223662 | ID=cds.MS.gene64636.t1;Parent=MS.gene64636.t1 |
| chr3.3 | exon | 30222400 | 30222578 | 30222400 | ID=MS.gene64636.t1.exon11;Parent=MS.gene64636.t1 |
| chr3.3 | CDS | 30222400 | 30222578 | 30222400 | ID=cds.MS.gene64636.t1;Parent=MS.gene64636.t1 |
| Gene Sequence |
| Protein sequence |
