AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66468


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66468.t1 MTR_1g017950 99.487 195 1 0 1 195 1 195 4.05e-146 404
MS.gene66468.t1 MTR_4g106800 66.857 175 57 1 2 176 3 176 1.70e-87 255
MS.gene66468.t1 MTR_3g070050 58.757 177 69 2 1 175 1 175 3.02e-70 212
MS.gene66468.t1 MTR_8g031490 50.820 183 83 3 1 182 1 177 2.64e-61 189
MS.gene66468.t1 MTR_8g097300 54.802 177 68 4 1 175 1 167 9.79e-61 188
MS.gene66468.t1 MTR_7g076720 50.857 175 82 2 1 175 1 171 3.28e-60 187
MS.gene66468.t1 MTR_3g070050 53.731 134 58 2 44 175 2 133 3.89e-45 146
MS.gene66468.t1 MTR_3g070050 47.826 69 36 0 3 71 61 129 1.42e-18 78.6
MS.gene66468.t1 MTR_1g106100 58.559 111 40 2 59 168 20 125 6.39e-38 128
MS.gene66468.t1 MTR_3g070033 66.038 53 18 0 123 175 6 58 3.58e-18 75.5
MS.gene66468.t1 MTR_3g070033 46.939 49 26 0 23 71 6 54 6.90e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66468.t1 AT1G10200 85.246 183 27 0 1 183 1 183 2.62e-116 328
MS.gene66468.t1 AT1G10200 82.014 139 25 0 45 183 3 141 2.77e-82 241
MS.gene66468.t1 AT1G10200 44.444 72 40 0 3 74 61 132 2.24e-16 72.8
MS.gene66468.t1 AT2G39900 58.192 177 69 2 1 175 1 174 2.13e-69 210
MS.gene66468.t1 AT3G55770 55.307 179 70 2 1 175 1 173 2.52e-67 205
MS.gene66468.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.85e-16 73.2
MS.gene66468.t1 AT3G55770 55.307 179 70 2 1 175 1 173 2.52e-67 205
MS.gene66468.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.85e-16 73.2
MS.gene66468.t1 AT3G55770 55.307 179 70 2 1 175 1 173 2.52e-67 205
MS.gene66468.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.85e-16 73.2
MS.gene66468.t1 AT3G55770 55.307 179 70 2 1 175 1 173 2.52e-67 205
MS.gene66468.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.85e-16 73.2
MS.gene66468.t1 AT2G45800 50.000 186 86 2 1 183 1 182 3.75e-61 190
MS.gene66468.t1 AT1G01780 48.649 185 87 3 1 183 1 179 2.17e-60 187
MS.gene66468.t1 AT3G61230 47.917 192 93 3 2 190 3 190 8.55e-60 186
MS.gene66468.t1 AT3G61230 36.792 106 67 0 3 108 100 205 3.58e-17 76.6
MS.gene66468.t1 AT3G55770 46.479 213 70 3 1 175 1 207 1.60e-59 186
MS.gene66468.t1 AT3G55770 43.678 87 49 0 103 189 3 89 1.44e-15 72.8
MS.gene66468.t1 AT3G55770 48.201 139 61 3 1 135 1 132 2.16e-40 134
MS.gene66468.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.56e-17 74.3
MS.gene66468.t1 AT2G45800 45.455 154 76 3 34 184 4 152 4.96e-39 132
MS.gene66468.t1 AT2G45800 50.725 69 34 0 3 71 68 136 1.35e-16 74.7
MS.gene66468.t1 AT3G55770 48.462 130 57 2 1 126 1 124 1.92e-38 129
MS.gene66468.t1 AT3G55770 43.678 87 49 0 103 189 3 89 5.92e-17 73.9

Find 36 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATTATCTATATTTCTTTC+TGG 0.117591 1.4:-5002181 None:intergenic
TCCCTCATGGGCTATGTAAT+TGG 0.293058 1.4:-5002748 None:intergenic
AGCATTTGAAACAACTCTTA+TGG 0.319973 1.4:-5002698 None:intergenic
TTTATAAAGGTTACAGTGAA+TGG 0.352930 1.4:+5002666 MS.gene66468:intron
TTTGCTTGATCAGTTGAATA+TGG 0.353364 1.4:-5002791 None:intergenic
CTTGAGGCTTTAGCTGCTGC+AGG 0.425078 1.4:-5002306 None:intergenic
TATTCAACTGATCAAGCAAA+AGG 0.432443 1.4:+5002794 MS.gene66468:CDS
GCTAAAGCCTCAAGTATGTT+TGG 0.441559 1.4:+5002315 MS.gene66468:CDS
GACCAACTGTTCAAAAGAAC+TGG 0.444337 1.4:+5002019 MS.gene66468:CDS
ACACTAATTACCTTCTCTGT+TGG 0.465114 1.4:-5002381 None:intergenic
GGAAATTTGAGCCAGCTTGA+AGG 0.475377 1.4:+5002816 MS.gene66468:CDS
GGAACAAGGGACAAATGTTC+TGG 0.477611 1.4:+5002339 MS.gene66468:CDS
TCTCATGGTCACCTTCAAGC+TGG 0.480325 1.4:-5002827 None:intergenic
CAAGTATGTTTGGTGGAACA+AGG 0.495604 1.4:+5002325 MS.gene66468:CDS
TTGAAACAACTCTTATGGTA+AGG 0.498185 1.4:-5002693 None:intergenic
AGAAAGAAATATAGATAATG+AGG 0.518674 1.4:+5002183 MS.gene66468:CDS
CCTTGAGGGTTCCTTTGCAG+TGG 0.520760 1.4:-5001543 None:intergenic
GAGAGAGACATAGACCTTGA+GGG 0.525903 1.4:-5001557 None:intergenic
GACTAATAGTACACCCTCCA+TGG 0.536605 1.4:-5002722 None:intergenic
AGGAACAACTCAGAAGTGTA+TGG 0.560932 1.4:+5001441 MS.gene66468:CDS
ATTCAACTGATCAAGCAAAA+GGG 0.562604 1.4:+5002795 MS.gene66468:CDS
AGTTGTTTCAAATGCTGCCA+TGG 0.564010 1.4:+5002705 MS.gene66468:CDS
GACATCTGAAACAAGCTTTG+TGG 0.565667 1.4:-5001519 None:intergenic
TTGAACAGTTGGTCGAAGTG+TGG 0.580292 1.4:-5002010 None:intergenic
TTCAGATGTCACCACTGCAA+AGG 0.582786 1.4:+5001532 MS.gene66468:CDS
GCTTGATCAGTTGAATATGG+TGG 0.582930 1.4:-5002788 None:intergenic
AAAGCCTCAAGTATGTTTGG+TGG 0.587944 1.4:+5002318 MS.gene66468:CDS
TCCAATTACATAGCCCATGA+GGG 0.590710 1.4:+5002747 MS.gene66468:CDS
GTTTCAAATGCTGCCATGGA+GGG 0.597804 1.4:+5002709 MS.gene66468:CDS
TGTTCCACCAAACATACTTG+AGG 0.606764 1.4:-5002322 None:intergenic
AACAGTGTATCCAACAGAGA+AGG 0.613878 1.4:+5002371 MS.gene66468:CDS
TTCCAATTACATAGCCCATG+AGG 0.614856 1.4:+5002746 MS.gene66468:CDS
TCATGGGCTATGTAATTGGA+AGG 0.623562 1.4:-5002744 None:intergenic
AAGTATGTTTGGTGGAACAA+GGG 0.641097 1.4:+5002326 MS.gene66468:CDS
CCACTGCAAAGGAACCCTCA+AGG 0.664342 1.4:+5001543 MS.gene66468:CDS
TGTTTCAAATGCTGCCATGG+AGG 0.668776 1.4:+5002708 MS.gene66468:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACTCAAAAATAATTTTGTT+CGG - chr1.4:5002531-5002550 None:intergenic 15.0%
!!! TCAATAATTTGCATTTATAA+AGG + chr1.4:5002653-5002672 MS.gene66468:intron 15.0%
!! AGAAAGAAATATAGATAATG+AGG + chr1.4:5002183-5002202 MS.gene66468:CDS 20.0%
!! GTTCATTAACATGAAAATTA+CGG - chr1.4:5001855-5001874 None:intergenic 20.0%
!! TCATTATCTATATTTCTTTC+TGG - chr1.4:5002184-5002203 None:intergenic 20.0%
! AAACAGCTCAAAAAATCATA+TGG - chr1.4:5002276-5002295 None:intergenic 25.0%
! AATGAACTTCTTTCATCATT+TGG + chr1.4:5001868-5001887 MS.gene66468:intron 25.0%
! AGGTAATGAATTCTAAATCT+AGG + chr1.4:5002203-5002222 MS.gene66468:intron 25.0%
! AGGTAATTAGTGTATATGTT+TGG + chr1.4:5002391-5002410 MS.gene66468:intron 25.0%
! CATAGCTTAAGTAAAAACTA+TGG + chr1.4:5001741-5001760 MS.gene66468:intron 25.0%
! GAAAATTGTTACTGCATATT+TGG - chr1.4:5001794-5001813 None:intergenic 25.0%
! TTAGTACATGTTTCAATAGA+CGG + chr1.4:5001668-5001687 MS.gene66468:intron 25.0%
! TTTATAAAGGTTACAGTGAA+TGG + chr1.4:5002666-5002685 MS.gene66468:intron 25.0%
!! AGTGTATATGTTTGGAATTA+CGG + chr1.4:5002399-5002418 MS.gene66468:intron 25.0%
!!! AAGTTTTGTATTCAACATCA+GGG - chr1.4:5002615-5002634 None:intergenic 25.0%
!!! ACTTTTGTTTTGCATACATA+GGG + chr1.4:5002140-5002159 MS.gene66468:intron 25.0%
!!! TAAGTTTTGTATTCAACATC+AGG - chr1.4:5002616-5002635 None:intergenic 25.0%
!!! TACTTTTGTTTTGCATACAT+AGG + chr1.4:5002139-5002158 MS.gene66468:intron 25.0%
!!! TGTAGCTTTTTCTAAAATCA+TGG + chr1.4:5002472-5002491 MS.gene66468:intron 25.0%
!!! TTTTGAGTTTGTGATTAGTT+TGG + chr1.4:5002542-5002561 MS.gene66468:intron 25.0%
AAAAATGCTGGGAAAATCAA+TGG + chr1.4:5002849-5002868 MS.gene66468:CDS 30.0%
ACCAAAAAGAAATGTCTAAG+AGG - chr1.4:5001587-5001606 None:intergenic 30.0%
ATTCAACTGATCAAGCAAAA+GGG + chr1.4:5002795-5002814 MS.gene66468:CDS 30.0%
CATTAACATGAAAATTACGG+TGG - chr1.4:5001852-5001871 None:intergenic 30.0%
TATTCAACTGATCAAGCAAA+AGG + chr1.4:5002794-5002813 MS.gene66468:CDS 30.0%
TGAAAATGCTTATGAACAAC+TGG - chr1.4:5001822-5001841 None:intergenic 30.0%
TTACTGCATATTTGGATTCA+CGG - chr1.4:5001786-5001805 None:intergenic 30.0%
TTGAAACAACTCTTATGGTA+AGG - chr1.4:5002696-5002715 None:intergenic 30.0%
TTTGCTTGATCAGTTGAATA+TGG - chr1.4:5002794-5002813 None:intergenic 30.0%
! AAGCAACTACAATTCTTTTG+AGG + chr1.4:5001970-5001989 MS.gene66468:CDS 30.0%
! ACCTCTTAGACATTTCTTTT+TGG + chr1.4:5001583-5001602 MS.gene66468:intron 30.0%
! AGCAACTACAATTCTTTTGA+GGG + chr1.4:5001971-5001990 MS.gene66468:CDS 30.0%
! AGCATTTGAAACAACTCTTA+TGG - chr1.4:5002701-5002720 None:intergenic 30.0%
! TTCTTTCATCATTTGGCTTA+TGG + chr1.4:5001875-5001894 MS.gene66468:intron 30.0%
!! AAAAGCTTTGAAGGTAACAA+AGG + chr1.4:5002052-5002071 MS.gene66468:intron 30.0%
!!! AGCTTTTTCTAAAATCATGG+TGG + chr1.4:5002475-5002494 MS.gene66468:intron 30.0%
!!! CTTTCTGGTTTGACAATTTT+TGG - chr1.4:5002169-5002188 None:intergenic 30.0%
!!! TACCTTCAAAGCTTTTTTCA+AGG - chr1.4:5002048-5002067 None:intergenic 30.0%
ACACTAATTACCTTCTCTGT+TGG - chr1.4:5002384-5002403 None:intergenic 35.0%
CTAACAGATCCAATACTAGA+AGG - chr1.4:5001631-5001650 None:intergenic 35.0%
! AAGTATGTTTGGTGGAACAA+GGG + chr1.4:5002326-5002345 MS.gene66468:CDS 35.0%
! AGCCTTGAAAAAAGCTTTGA+AGG + chr1.4:5002043-5002062 MS.gene66468:CDS 35.0%
! CTAGTTTAGCCTTCTAGTAT+TGG + chr1.4:5001619-5001638 MS.gene66468:intron 35.0%
! TACCAGTTCTTTTGAACAGT+TGG - chr1.4:5002024-5002043 None:intergenic 35.0%
! TTTCATCATTTGGCTTATGG+AGG + chr1.4:5001878-5001897 MS.gene66468:intron 35.0%
! TTTTCCCAGCATTTTTCTCA+TGG - chr1.4:5002845-5002864 None:intergenic 35.0%
!! CGGCTAGTTTAACAATATCA+CGG + chr1.4:5002419-5002438 MS.gene66468:intron 35.0%
AAAGCCTCAAGTATGTTTGG+TGG + chr1.4:5002318-5002337 MS.gene66468:CDS 40.0%
AACAGTGTATCCAACAGAGA+AGG + chr1.4:5002371-5002390 MS.gene66468:CDS 40.0%
GACCAACTGTTCAAAAGAAC+TGG + chr1.4:5002019-5002038 MS.gene66468:CDS 40.0%
GCTAAAGCCTCAAGTATGTT+TGG + chr1.4:5002315-5002334 MS.gene66468:CDS 40.0%
GCTTGATCAGTTGAATATGG+TGG - chr1.4:5002791-5002810 None:intergenic 40.0%
GTGACCATGAGAAAAATGCT+GGG + chr1.4:5002838-5002857 MS.gene66468:CDS 40.0%
GTTCGGATTTGTGATGAGTT+TGG - chr1.4:5002514-5002533 None:intergenic 40.0%
TCCAATTACATAGCCCATGA+GGG + chr1.4:5002747-5002766 MS.gene66468:CDS 40.0%
TGCAACTTTCGCCAAAATCA+CGG - chr1.4:5002586-5002605 None:intergenic 40.0%
TGTTCCACCAAACATACTTG+AGG - chr1.4:5002325-5002344 None:intergenic 40.0%
TTCCAATTACATAGCCCATG+AGG + chr1.4:5002746-5002765 MS.gene66468:CDS 40.0%
! AGGAACAACTCAGAAGTGTA+TGG + chr1.4:5001441-5001460 MS.gene66468:CDS 40.0%
! AGTTGTTTCAAATGCTGCCA+TGG + chr1.4:5002705-5002724 MS.gene66468:CDS 40.0%
! CAAGTATGTTTGGTGGAACA+AGG + chr1.4:5002325-5002344 MS.gene66468:CDS 40.0%
! GACATCTGAAACAAGCTTTG+TGG - chr1.4:5001522-5001541 None:intergenic 40.0%
! TCATGGGCTATGTAATTGGA+AGG - chr1.4:5002747-5002766 None:intergenic 40.0%
GACTAATAGTACACCCTCCA+TGG - chr1.4:5002725-5002744 None:intergenic 45.0%
GAGAGAGACATAGACCTTGA+GGG - chr1.4:5001560-5001579 None:intergenic 45.0%
GATGAGTTTGGCAGAATCTG+TGG - chr1.4:5002502-5002521 None:intergenic 45.0%
GGAAATTTGAGCCAGCTTGA+AGG + chr1.4:5002816-5002835 MS.gene66468:CDS 45.0%
GGAACAAGGGACAAATGTTC+TGG + chr1.4:5002339-5002358 MS.gene66468:CDS 45.0%
GGTGACCATGAGAAAAATGC+TGG + chr1.4:5002837-5002856 MS.gene66468:CDS 45.0%
GTTTCAAATGCTGCCATGGA+GGG + chr1.4:5002709-5002728 MS.gene66468:CDS 45.0%
TGAGAGAGACATAGACCTTG+AGG - chr1.4:5001561-5001580 None:intergenic 45.0%
TGTTTCAAATGCTGCCATGG+AGG + chr1.4:5002708-5002727 MS.gene66468:CDS 45.0%
TTCAGATGTCACCACTGCAA+AGG + chr1.4:5001532-5001551 MS.gene66468:CDS 45.0%
TTGAACAGTTGGTCGAAGTG+TGG - chr1.4:5002013-5002032 None:intergenic 45.0%
! GCAGTAGAGTTTTCCCTCAT+GGG - chr1.4:5002763-5002782 None:intergenic 45.0%
! TCATTGTGCGACCGTGATTT+TGG + chr1.4:5002572-5002591 MS.gene66468:intron 45.0%
! TCCCTCATGGGCTATGTAAT+TGG - chr1.4:5002751-5002770 None:intergenic 45.0%
! TGCAGTAGAGTTTTCCCTCA+TGG - chr1.4:5002764-5002783 None:intergenic 45.0%
TCTCATGGTCACCTTCAAGC+TGG - chr1.4:5002830-5002849 None:intergenic 50.0%
CCACTGCAAAGGAACCCTCA+AGG + chr1.4:5001543-5001562 MS.gene66468:CDS 55.0%
CCTTGAGGGTTCCTTTGCAG+TGG - chr1.4:5001546-5001565 None:intergenic 55.0%
! CTTGAGGCTTTAGCTGCTGC+AGG - chr1.4:5002309-5002328 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 5001430 5002896 5001430 ID=MS.gene66468
chr1.4 mRNA 5001430 5002896 5001430 ID=MS.gene66468.t1;Parent=MS.gene66468
chr1.4 exon 5001430 5001564 5001430 ID=MS.gene66468.t1.exon1;Parent=MS.gene66468.t1
chr1.4 CDS 5001430 5001564 5001430 ID=cds.MS.gene66468.t1;Parent=MS.gene66468.t1
chr1.4 exon 5001968 5002064 5001968 ID=MS.gene66468.t1.exon2;Parent=MS.gene66468.t1
chr1.4 CDS 5001968 5002064 5001968 ID=cds.MS.gene66468.t1;Parent=MS.gene66468.t1
chr1.4 exon 5002161 5002204 5002161 ID=MS.gene66468.t1.exon3;Parent=MS.gene66468.t1
chr1.4 CDS 5002161 5002204 5002161 ID=cds.MS.gene66468.t1;Parent=MS.gene66468.t1
chr1.4 exon 5002303 5002392 5002303 ID=MS.gene66468.t1.exon4;Parent=MS.gene66468.t1
chr1.4 CDS 5002303 5002392 5002303 ID=cds.MS.gene66468.t1;Parent=MS.gene66468.t1
chr1.4 exon 5002675 5002896 5002675 ID=MS.gene66468.t1.exon5;Parent=MS.gene66468.t1
chr1.4 CDS 5002675 5002896 5002675 ID=cds.MS.gene66468.t1;Parent=MS.gene66468.t1
Gene Sequence

>MS.gene66468

ATGGCATTTGCAGGAACAACTCAGAAGTGTATGGCTTGTAGCAAAACAGTTTATCTTGTTGATAAGTTAACTGCTGATAATAGAATTTTCCACAAAGCTTGTTTCAGATGTCACCACTGCAAAGGAACCCTCAAGGTCTATGTCTCTCTCATAACCTCTTAGACATTTCTTTTTGGTCTTATATAGTCTCTAGTTTAGCCTTCTAGTATTGGATCTGTTAGAATCATAATCATCAAATTTAGTACATGTTTCAATAGACGGTGAGTTTAGCGAAATGACAATAACACTGTAATTTTATTGAAGCTACAAAGCATAGCTTAAGTAAAAACTATGGTAGCACACAATGAAAATCGCCGTGAATCCAAATATGCAGTAACAATTTTCTTTAACCAGTTGTTCATAAGCATTTTCATCTCAGACCACCGTAATTTTCATGTTAATGAACTTCTTTCATCATTTGGCTTATGGAGGTATGTTACTACATTGAAATAATTTTCATATACAAGTTTGCTGATAGATTAACGTATATACAATTCAGCTAAGCAACTACAATTCTTTTGAGGGAGTTCTTTACTGCAGACCACACTTCGACCAACTGTTCAAAAGAACTGGTAGCCTTGAAAAAAGCTTTGAAGGTAACAAAGGATACATAACTGAATTTGTTAATTAAATGTGTCACAGTACTTTTTTCTTGTCAGTGTCTAAATTGTACTTTTGTTTTGCATACATAGGGACACCAAAAATTGTCAAACCAGAAAGAAATATAGATAATGAGGTAATGAATTCTAAATCTAGGTGTTTACTGTTTACAATTCACAGTAGTAAATTCATGTTTTCAGCATTCCATATGATTTTTTGAGCTGTTTTAATCAGAAACCTGCAGCAGCTAAAGCCTCAAGTATGTTTGGTGGAACAAGGGACAAATGTTCTGGTTGTCAGAAAACAGTGTATCCAACAGAGAAGGTAATTAGTGTATATGTTTGGAATTACGGCTAGTTTAACAATATCACGGTGACACTGTAATTTTGTTGTAATTCTAAAGTGTAGCTTTTTCTAAAATCATGGTGGACCACAGATTCTGCCAAACTCATCACAAATCCGAACAAAATTATTTTTGAGTTTGTGATTAGTTTGGCAAAATCTCATTGTGCGACCGTGATTTTGGCGAAAGTTGCATCTAAACCCTGATGTTGAATACAAAACTTATTGTTCTACTAATGAAATCAATAATTTGCATTTATAAAGGTTACAGTGAATGGAACTCCTTACCATAAGAGTTGTTTCAAATGCTGCCATGGAGGGTGTACTATTAGTCCTTCCAATTACATAGCCCATGAGGGAAAACTCTACTGCAAACACCACCATATTCAACTGATCAAGCAAAAGGGAAATTTGAGCCAGCTTGAAGGTGACCATGAGAAAAATGCTGGGAAAATCAATGGCGAAGAAGTTGCAGCTGAAACTTGA

Protein sequence

>MS.gene66468.t1

MAFAGTTQKCMACSKTVYLVDKLTADNRIFHKACFRCHHCKGTLKLSNYNSFEGVLYCRPHFDQLFKRTGSLEKSFEGTPKIVKPERNIDNEKPAAAKASSMFGGTRDKCSGCQKTVYPTEKVTVNGTPYHKSCFKCCHGGCTISPSNYIAHEGKLYCKHHHIQLIKQKGNLSQLEGDHEKNAGKINGEEVAAET