AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68213


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68213.t1 MTR_3g116830 96.629 178 6 0 1 178 1 178 4.12e-128 357
MS.gene68213.t1 MTR_1g054750 26.437 174 94 5 32 171 12 185 4.30e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68213.t1 AT2G21620 76.404 178 41 1 1 178 1 177 7.64e-93 268
MS.gene68213.t1 AT2G21620 73.913 184 41 2 1 178 1 183 3.25e-90 262

Find 43 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCTTGTTCATGCTGTTTC+TGG 0.024473 3.1:-92165279 MS.gene68213:intron
TAAACCTGCAGCTGTGGTTT+TGG 0.252116 3.1:-92164307 MS.gene68213:CDS
GCACCTGTTGTGATCGTTCC+TGG 0.338638 3.1:-92164086 MS.gene68213:CDS
TCAAAAGCATGTTTACTGTT+TGG 0.339328 3.1:+92165342 None:intergenic
CCAGAGCTTGAGAGGGAAAC+AGG 0.363491 3.1:-92165411 MS.gene68213:CDS
AGCTCTGGCTCAGGCACTAT+TGG 0.369995 3.1:+92165426 None:intergenic
ACTGTTTGGACCATGATCTA+TGG 0.408420 3.1:+92165356 None:intergenic
TTGAAACAAGCTTCTGCCTC+TGG 0.408871 3.1:+92164281 None:intergenic
GTGATGCATCAATGGTCTAA+TGG 0.411227 3.1:-92163971 None:intergenic
CCTGTTTCCCTCTCAAGCTC+TGG 0.412478 3.1:+92165411 None:intergenic
CGACTTAACCCAGGGATTGA+TGG 0.429955 3.1:-92165088 MS.gene68213:CDS
CTAATTTCTCCATCAATCCC+TGG 0.431733 3.1:+92165079 None:intergenic
TCCCTCTCAAGCTCTGGCTC+AGG 0.455326 3.1:+92165417 None:intergenic
TTTCCAGGAACGATCACAAC+AGG 0.474556 3.1:+92164083 None:intergenic
CAGGAGAAAGAAGAAGAGGA+AGG 0.480520 3.1:-92165392 MS.gene68213:CDS
GAGGCAGAAGCTTGTTTCAA+AGG 0.482260 3.1:-92164278 MS.gene68213:intron
GAAACAGGAGAAAGAAGAAG+AGG 0.483483 3.1:-92165396 MS.gene68213:CDS
CGGATAGTGCAAGGTGATGC+AGG 0.488351 3.1:-92164360 MS.gene68213:CDS
GTCCCCAAAACCACAGCTGC+AGG 0.501479 3.1:+92164303 None:intergenic
GTTGTGATCGTTCCTGGAAA+AGG 0.523884 3.1:-92164080 MS.gene68213:intron
TCCTTCTTCAGTGTGCTACA+AGG 0.530640 3.1:-92164146 MS.gene68213:intron
AGGAGAAAGAAGAAGAGGAA+GGG 0.532412 3.1:-92165391 MS.gene68213:CDS
TAAATTAATGGAGACAGTGA+TGG 0.533156 3.1:-92165499 None:intergenic
TAATTTCTCCATCAATCCCT+GGG 0.538350 3.1:+92165080 None:intergenic
TGCCTGAGCCAGAGCTTGAG+AGG 0.541192 3.1:-92165419 MS.gene68213:CDS
AGATGCTGGTGATGCATCAA+TGG 0.543889 3.1:-92163979 MS.gene68213:CDS
ATAGTATTAGCCATAGATCA+TGG 0.553466 3.1:-92165366 MS.gene68213:CDS
AACAGGTGAAAACAGTAGCT+CGG 0.557174 3.1:-92164380 MS.gene68213:intron
GGCACTATTGGTATCAGAGA+AGG 0.564937 3.1:+92165438 None:intergenic
GAGGATTAAACCTGCAGCTG+TGG 0.569878 3.1:-92164313 MS.gene68213:CDS
GATGGAGAAATTAGCGGCTG+AGG 0.575246 3.1:-92165070 MS.gene68213:CDS
GGGATTGATGGAGAAATTAG+CGG 0.601178 3.1:-92165076 MS.gene68213:CDS
AACCAGAAACAGCATGAACA+AGG 0.607545 3.1:+92165277 None:intergenic
GCCTGAGCCAGAGCTTGAGA+GGG 0.610344 3.1:-92165418 MS.gene68213:CDS
TGCAGGAAAAGTAATTTGCA+AGG 0.617105 3.1:-92164343 MS.gene68213:CDS
AACAGCATGAACAAGGTGGA+TGG 0.627059 3.1:+92165284 None:intergenic
ACAGTAGCTCGGATAGTGCA+AGG 0.631985 3.1:-92164369 MS.gene68213:CDS
ATTGTGTACGACTTAACCCA+GGG 0.659770 3.1:-92165096 MS.gene68213:CDS
AATTGTGTACGACTTAACCC+AGG 0.666749 3.1:-92165097 MS.gene68213:CDS
CAGAAACAGCATGAACAAGG+TGG 0.686182 3.1:+92165280 None:intergenic
CAACAGGTGCTGCTTTACAG+TGG 0.687014 3.1:+92164099 None:intergenic
AGTGTGCTACAAGGAAGCGT+CGG 0.687872 3.1:-92164137 MS.gene68213:CDS
TAATTTGCAAGGAAGCAGAG+AGG 0.695628 3.1:-92164332 MS.gene68213:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACTTCAATTAATTTATA+TGG + chr3.1:92164272-92164291 None:intergenic 10.0%
!!! GATTTTTTTTTATTACTTTA+TGG - chr3.1:92164302-92164321 MS.gene68213:CDS 10.0%
!!! TTATTGTGAATTTATTTTTT+TGG - chr3.1:92164970-92164989 MS.gene68213:intron 10.0%
!! AAAAAACATATACAGAATAA+TGG + chr3.1:92165299-92165318 None:intergenic 15.0%
!! TCCAATTTGAAAAATTATAT+AGG - chr3.1:92164472-92164491 MS.gene68213:intron 15.0%
!!! AAAACTTATTCTTTTTCAAT+GGG - chr3.1:92164857-92164876 MS.gene68213:intron 15.0%
!!! AATTGAAGTTTTTGTTTTTA+TGG - chr3.1:92164280-92164299 MS.gene68213:CDS 15.0%
!!! TATTACAAGTTGATTTTATA+CGG + chr3.1:92164806-92164825 None:intergenic 15.0%
!!! TCCTATATAATTTTTCAAAT+TGG + chr3.1:92164476-92164495 None:intergenic 15.0%
!! ATCACATGTAAAAAAAACAA+TGG - chr3.1:92164606-92164625 MS.gene68213:intron 20.0%
!! GCATATCTTGAAATATTTAA+TGG - chr3.1:92164655-92164674 MS.gene68213:intron 20.0%
!! TAAAATCAACTTGTAATACA+AGG - chr3.1:92164808-92164827 MS.gene68213:intron 20.0%
!! TATCTTGAAATATTTAATGG+AGG - chr3.1:92164658-92164677 MS.gene68213:intron 20.0%
!! TCACATGTAAAAAAAACAAT+GGG - chr3.1:92164607-92164626 MS.gene68213:intron 20.0%
!! TTCATAAGTAAATAGGAAAA+CGG + chr3.1:92164451-92164470 None:intergenic 20.0%
!!! CAAAACTTATTCTTTTTCAA+TGG - chr3.1:92164856-92164875 MS.gene68213:intron 20.0%
! AAAAGTTTAATCAACCATCA+TGG - chr3.1:92164714-92164733 MS.gene68213:intron 25.0%
! ACAAATTGTGGTTAATGATT+TGG + chr3.1:92164549-92164568 None:intergenic 25.0%
! ATTGGACTTCATAAGTAAAT+AGG + chr3.1:92164458-92164477 None:intergenic 25.0%
! CAATAGCATAATTAAGTCAA+GGG - chr3.1:92164633-92164652 MS.gene68213:intron 25.0%
! CACATGTAAAAAAAACAATG+GGG - chr3.1:92164608-92164627 MS.gene68213:intron 25.0%
! CATACTAATCATAAGTAGAA+TGG + chr3.1:92165059-92165078 None:intergenic 25.0%
! GGGATTATTAATACATGTAA+AGG + chr3.1:92164894-92164913 None:intergenic 25.0%
! TAATGAGTGATAAACAATAG+AGG + chr3.1:92165000-92165019 None:intergenic 25.0%
! TCAATAGCATAATTAAGTCA+AGG - chr3.1:92164632-92164651 MS.gene68213:intron 25.0%
AGTTATCAATAAATGCTGCA+AGG + chr3.1:92164915-92164934 None:intergenic 30.0%
ATAGTATTAGCCATAGATCA+TGG - chr3.1:92164100-92164119 MS.gene68213:CDS 30.0%
ATGAGAAGTTAAGAAAGTGA+AGG + chr3.1:92164223-92164242 None:intergenic 30.0%
GAGGTCAAAGATACTAATTA+CGG - chr3.1:92164677-92164696 MS.gene68213:intron 30.0%
GATTAGTATGATGTGAAAAC+AGG - chr3.1:92165069-92165088 MS.gene68213:CDS 30.0%
GTTATCAATAAATGCTGCAA+GGG + chr3.1:92164914-92164933 None:intergenic 30.0%
TTCAATCCCTTTCTATATCT+CGG - chr3.1:92165249-92165268 MS.gene68213:intron 30.0%
! ACAGTAAACATGCTTTTGAT+TGG - chr3.1:92164128-92164147 MS.gene68213:CDS 30.0%
! GCAGCATTTATTGATAACTT+TGG - chr3.1:92164916-92164935 MS.gene68213:intron 30.0%
! TCAAAAGCATGTTTACTGTT+TGG + chr3.1:92164127-92164146 None:intergenic 30.0%
!! ATACTAATTACGGTGGATTA+GGG - chr3.1:92164687-92164706 MS.gene68213:intron 30.0%
!! CAGTAAACATGCTTTTGATT+GGG - chr3.1:92164129-92164148 MS.gene68213:CDS 30.0%
CTCATTACTACTCTATTTCC+TGG - chr3.1:92165013-92165032 MS.gene68213:intron 35.0%
GTCAAAGATACTAATTACGG+TGG - chr3.1:92164680-92164699 MS.gene68213:intron 35.0%
TAATTTCTCCATCAATCCCT+GGG + chr3.1:92164389-92164408 None:intergenic 35.0%
TACTCTATTTCCTGGCAAAT+TGG - chr3.1:92165021-92165040 MS.gene68213:intron 35.0%
! GATACTAATTACGGTGGATT+AGG - chr3.1:92164686-92164705 MS.gene68213:intron 35.0%
! TGCAGGAAAAGTAATTTGCA+AGG - chr3.1:92165123-92165142 MS.gene68213:intron 35.0%
!!! TTCTTTTTCAATGGGAGACT+TGG - chr3.1:92164865-92164884 MS.gene68213:intron 35.0%
AAATGGACAACTCCGTGAAT+GGG + chr3.1:92164574-92164593 None:intergenic 40.0%
AACAGGTGAAAACAGTAGCT+CGG - chr3.1:92165086-92165105 MS.gene68213:CDS 40.0%
AACCAGAAACAGCATGAACA+AGG + chr3.1:92164192-92164211 None:intergenic 40.0%
AATTGTGTACGACTTAACCC+AGG - chr3.1:92164369-92164388 MS.gene68213:CDS 40.0%
ACTGTTTGGACCATGATCTA+TGG + chr3.1:92164113-92164132 None:intergenic 40.0%
AGAATGGCCGAGATATAGAA+AGG + chr3.1:92165259-92165278 None:intergenic 40.0%
AGGAGAAAGAAGAAGAGGAA+GGG - chr3.1:92164075-92164094 MS.gene68213:intron 40.0%
ATTCACGGAGTTGTCCATTT+AGG - chr3.1:92164574-92164593 MS.gene68213:intron 40.0%
ATTGTGTACGACTTAACCCA+GGG - chr3.1:92164370-92164389 MS.gene68213:CDS 40.0%
CACAATTTGTGTCCCATTCA+CGG - chr3.1:92164559-92164578 MS.gene68213:intron 40.0%
CTAATTTCTCCATCAATCCC+TGG + chr3.1:92164390-92164409 None:intergenic 40.0%
CTTCTTTGATTGACAGATGC+TGG - chr3.1:92165473-92165492 MS.gene68213:CDS 40.0%
GAAACAGGAGAAAGAAGAAG+AGG - chr3.1:92164070-92164089 MS.gene68213:intron 40.0%
GAACAACTTGTAAGCCATGA+TGG + chr3.1:92164731-92164750 None:intergenic 40.0%
GAATAGATTGCCAATTTGCC+AGG + chr3.1:92165034-92165053 None:intergenic 40.0%
GAATGGCCGAGATATAGAAA+GGG + chr3.1:92165258-92165277 None:intergenic 40.0%
GGGATTGATGGAGAAATTAG+CGG - chr3.1:92164390-92164409 MS.gene68213:intron 40.0%
GTGATTGACTACCACCTAAA+TGG + chr3.1:92164591-92164610 None:intergenic 40.0%
TAAATGGACAACTCCGTGAA+TGG + chr3.1:92164575-92164594 None:intergenic 40.0%
TAACTGCAGTGTGTAGAGAA+TGG + chr3.1:92165275-92165294 None:intergenic 40.0%
! TAATTTGCAAGGAAGCAGAG+AGG - chr3.1:92165134-92165153 MS.gene68213:intron 40.0%
! TAGTAATGCACACCTTTTCC+AGG + chr3.1:92165401-92165420 None:intergenic 40.0%
!! TTGTTCATGCTGTTTCTGGT+TGG - chr3.1:92164191-92164210 MS.gene68213:intron 40.0%
!!! TGAGGCTTTTGAAGTTGCAA+TGG - chr3.1:92164414-92164433 MS.gene68213:intron 40.0%
AACAGCATGAACAAGGTGGA+TGG + chr3.1:92164185-92164204 None:intergenic 45.0%
CAGAAACAGCATGAACAAGG+TGG + chr3.1:92164189-92164208 None:intergenic 45.0%
CAGGAGAAAGAAGAAGAGGA+AGG - chr3.1:92164074-92164093 MS.gene68213:intron 45.0%
CGTGAATGGGACACAAATTG+TGG + chr3.1:92164561-92164580 None:intergenic 45.0%
GGCACTATTGGTATCAGAGA+AGG + chr3.1:92164031-92164050 None:intergenic 45.0%
GTTGTGATCGTTCCTGGAAA+AGG - chr3.1:92165386-92165405 MS.gene68213:CDS 45.0%
TCCTTCTTCAGTGTGCTACA+AGG - chr3.1:92165320-92165339 MS.gene68213:CDS 45.0%
TCCTTGTAGCACACTGAAGA+AGG + chr3.1:92165324-92165343 None:intergenic 45.0%
TTGAAACAAGCTTCTGCCTC+TGG + chr3.1:92165188-92165207 None:intergenic 45.0%
TTTCCAGGAACGATCACAAC+AGG + chr3.1:92165386-92165405 None:intergenic 45.0%
! AAACCTGCAGCTGTGGTTTT+GGG - chr3.1:92165160-92165179 MS.gene68213:intron 45.0%
! AGATGCTGGTGATGCATCAA+TGG - chr3.1:92165487-92165506 MS.gene68213:CDS 45.0%
! GAGGCAGAAGCTTGTTTCAA+AGG - chr3.1:92165188-92165207 MS.gene68213:intron 45.0%
! TAAACCTGCAGCTGTGGTTT+TGG - chr3.1:92165159-92165178 MS.gene68213:intron 45.0%
!! CACCTTGTTCATGCTGTTTC+TGG - chr3.1:92164187-92164206 MS.gene68213:intron 45.0%
!! TCATGCTGTTTCTGGTTGGT+TGG - chr3.1:92164195-92164214 MS.gene68213:intron 45.0%
!! AAAAAAAATAAAAATAAACA+TGG - chr3.1:92164521-92164540 MS.gene68213:intron 5.0%
ACAGTAGCTCGGATAGTGCA+AGG - chr3.1:92165097-92165116 MS.gene68213:CDS 50.0%
CACGGAGTTGTCCATTTAGG+TGG - chr3.1:92164577-92164596 MS.gene68213:intron 50.0%
CGACTTAACCCAGGGATTGA+TGG - chr3.1:92164378-92164397 MS.gene68213:CDS 50.0%
GAGGATTAAACCTGCAGCTG+TGG - chr3.1:92165153-92165172 MS.gene68213:intron 50.0%
GATGGAGAAATTAGCGGCTG+AGG - chr3.1:92164396-92164415 MS.gene68213:intron 50.0%
GTGAGCGTGTGTGTGTGTAT+GGG - chr3.1:92165210-92165229 MS.gene68213:intron 50.0%
! AGTGTGCTACAAGGAAGCGT+CGG - chr3.1:92165329-92165348 MS.gene68213:CDS 50.0%
!! AACCTGCAGCTGTGGTTTTG+GGG - chr3.1:92165161-92165180 MS.gene68213:intron 50.0%
!! CAACAGGTGCTGCTTTACAG+TGG + chr3.1:92165370-92165389 None:intergenic 50.0%
CCAGAGCTTGAGAGGGAAAC+AGG - chr3.1:92164055-92164074 MS.gene68213:intron 55.0%
CCTGTTTCCCTCTCAAGCTC+TGG + chr3.1:92164058-92164077 None:intergenic 55.0%
CGGATAGTGCAAGGTGATGC+AGG - chr3.1:92165106-92165125 MS.gene68213:CDS 55.0%
GCACCTGTTGTGATCGTTCC+TGG - chr3.1:92165380-92165399 MS.gene68213:CDS 55.0%
GGTGAGCGTGTGTGTGTGTA+TGG - chr3.1:92165209-92165228 MS.gene68213:intron 55.0%
! AGCTCTGGCTCAGGCACTAT+TGG + chr3.1:92164043-92164062 None:intergenic 55.0%
GCCTGAGCCAGAGCTTGAGA+GGG - chr3.1:92164048-92164067 MS.gene68213:intron 60.0%
GTCCCCAAAACCACAGCTGC+AGG + chr3.1:92165166-92165185 None:intergenic 60.0%
TGCCTGAGCCAGAGCTTGAG+AGG - chr3.1:92164047-92164066 MS.gene68213:intron 60.0%
! TCCCTCTCAAGCTCTGGCTC+AGG + chr3.1:92164052-92164071 None:intergenic 60.0%
!!! GCTGTGGTTTTGGGGACCAG+AGG - chr3.1:92165169-92165188 MS.gene68213:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 92163974 92165514 92163974 ID=MS.gene68213
chr3.1 mRNA 92163974 92165514 92163974 ID=MS.gene68213.t1;Parent=MS.gene68213
chr3.1 exon 92165280 92165514 92165280 ID=MS.gene68213.t1.exon1;Parent=MS.gene68213.t1
chr3.1 CDS 92165280 92165514 92165280 ID=cds.MS.gene68213.t1;Parent=MS.gene68213.t1
chr3.1 exon 92165053 92165132 92165053 ID=MS.gene68213.t1.exon2;Parent=MS.gene68213.t1
chr3.1 CDS 92165053 92165132 92165053 ID=cds.MS.gene68213.t1;Parent=MS.gene68213.t1
chr3.1 exon 92164279 92164397 92164279 ID=MS.gene68213.t1.exon3;Parent=MS.gene68213.t1
chr3.1 CDS 92164279 92164397 92164279 ID=cds.MS.gene68213.t1;Parent=MS.gene68213.t1
chr3.1 exon 92164081 92164157 92164081 ID=MS.gene68213.t1.exon4;Parent=MS.gene68213.t1
chr3.1 CDS 92164081 92164157 92164081 ID=cds.MS.gene68213.t1;Parent=MS.gene68213.t1
chr3.1 exon 92163974 92163999 92163974 ID=MS.gene68213.t1.exon5;Parent=MS.gene68213.t1
chr3.1 CDS 92163974 92163999 92163974 ID=cds.MS.gene68213.t1;Parent=MS.gene68213.t1
Gene Sequence

>MS.gene68213

ATGGAGACAGTGATGGAAGATGTAGAAGAATACAACTACAGAGAAGTGAAGCTTCCTTCTCTGATACCAATAGTGCCTGAGCCAGAGCTTGAGAGGGAAACAGGAGAAAGAAGAAGAGGAAGGGATATAGTATTAGCCATAGATCATGGTCCAAACAGTAAACATGCTTTTGATTGGGCTCTTATTCATCTATGCAGACTTGCTGATACCATCCACCTTGTTCATGCTGTTTCTGGTTGGTTGGCTCCTTCACTTTCTTAACTTCTCATATATTACTTTTTTAATTTATTTTGTTCCATATAAATTAATTGAAGTTTTTGTTTTTATGGATTTTTTTTTATTACTTTATGGATATTTATTTTGTTAATGAAATATTTTGCAGATGTGAAGAATCAAATTGTGTACGACTTAACCCAGGGATTGATGGAGAAATTAGCGGCTGAGGCTTTTGAAGTTGCAATGGTTAGTGTCTCTCCGTTTTCCTATTTACTTATGAAGTCCAATTTGAAAAATTATATAGGATAGAGTTAGAGTTGTTCAATTTGACAAAAAAAATAAAAATAAACATGGCTCCAAATCATTAACCACAATTTGTGTCCCATTCACGGAGTTGTCCATTTAGGTGGTAGTCAATCACATGTAAAAAAAACAATGGGGTTCAATAGCATAATTAAGTCAAGGGCATATCTTGAAATATTTAATGGAGGTCAAAGATACTAATTACGGTGGATTAGGGAAAAAAAAGTTTAATCAACCATCATGGCTTACAAGTTGTTCAGTCACTTCATTTTTTAGATGATTAAGTTTTATATGAAGTCTGACGACTCATCCGTATAAAATCAACTTGTAATACAAGGAATGTCACTCTTTATAATCTGTTTACAAAACTTATTCTTTTTCAATGGGAGACTTGGAGTCCTTTACATGTATTAATAATCCCTTGCAGCATTTATTGATAACTTTGGTGCTTTATTAGTTGAATATGATGAATGCTAATTATTGTGAATTTATTTTTTTGGTTAACCTCTATTGTTTATCACTCATTACTACTCTATTTCCTGGCAAATTGGCAATCTATTCTTCCATTCTACTTATGATTAGTATGATGTGAAAACAGGTGAAAACAGTAGCTCGGATAGTGCAAGGTGATGCAGGAAAAGTAATTTGCAAGGAAGCAGAGAGGATTAAACCTGCAGCTGTGGTTTTGGGGACCAGAGGCAGAAGCTTGTTTCAAAGGTGAGCGTGTGTGTGTGTATGGGTGTACATTACATTCAATTCAATCCCTTTCTATATCTCGGCCATTCTCTACACACTGCAGTTATCCATTATTCTGTATATGTTTTTTTTCCTTCTTCAGTGTGCTACAAGGAAGCGTCGGTGAGTATTGCTTTCACCACTGTAAAGCAGCACCTGTTGTGATCGTTCCTGGAAAAGGTGTGCATTACTATTCTTTTTATCATTTTACTTTTACTCAAGTTTAGAATACTGAAGAAGTTTAGCTTCTTTGATTGACAGATGCTGGTGATGCATCAATGGTCTAA

Protein sequence

>MS.gene68213.t1

METVMEDVEEYNYREVKLPSLIPIVPEPELERETGERRRGRDIVLAIDHGPNSKHAFDWALIHLCRLADTIHLVHAVSDVKNQIVYDLTQGLMEKLAAEAFEVAMVKTVARIVQGDAGKVICKEAERIKPAAVVLGTRGRSLFQSVLQGSVGEYCFHHCKAAPVVIVPGKDAGDASMV