AlfalfaGEDB | MS.gene68216

M. sativa cultivar XinJiangDaYe / MS.gene68216

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00076.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68216.t1	XP_027353977.1	92.9	85	6	0	2	86	4	88	6.20E-37	164.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68216.t1	Q03878	81.4	86	16	0	1	86	1	86	1.7e-35	150.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68216.t1	A0A2K3P4T0	91.9	86	7	0	1	86	1	86	7.6e-37	163.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049129	MS.gene68216	-0.813555	2.41E-51	-1.69E-46
MS.gene049411	MS.gene68216	0.821365	4.20E-53	-1.69E-46
MS.gene050752	MS.gene68216	0.818624	1.78E-52	-1.69E-46
MS.gene050945	MS.gene68216	0.81142	7.06E-51	-1.69E-46
MS.gene050955	MS.gene68216	0.820288	7.42E-53	-1.69E-46
MS.gene050985	MS.gene68216	0.820922	5.31E-53	-1.69E-46
MS.gene051234	MS.gene68216	0.835608	1.52E-56	-1.69E-46
MS.gene052793	MS.gene68216	0.804339	2.26E-49	-1.69E-46
MS.gene054403	MS.gene68216	0.826568	2.53E-54	-1.69E-46
MS.gene054413	MS.gene68216	0.813284	2.77E-51	-1.69E-46
MS.gene055555	MS.gene68216	0.809361	1.97E-50	-1.69E-46
MS.gene056075	MS.gene68216	0.839769	1.30E-57	-1.69E-46
MS.gene057684	MS.gene68216	-0.809215	2.11E-50	-1.69E-46
MS.gene057705	MS.gene68216	0.818647	1.76E-52	-1.69E-46
MS.gene058245	MS.gene68216	0.807662	4.53E-50	-1.69E-46
MS.gene059802	MS.gene68216	0.801113	1.05E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68216.t1	MTR_4g070080	86.022	93	13	0	1	93	1	93	2.40e-49	156
MS.gene68216.t1	MTR_4g070080	84.337	83	13	0	1	83	1	83	6.33e-49	156
MS.gene68216.t1	MTR_4g070140	80.723	83	16	0	2	84	4	86	1.46e-45	149
MS.gene68216.t1	MTR_4g070190	72.414	116	15	1	2	117	4	102	3.12e-42	139
MS.gene68216.t1	MTR_3g084040	53.165	79	36	1	7	85	37	114	6.17e-24	92.0
MS.gene68216.t1	MTR_3g075500	53.571	84	39	0	3	86	203	286	1.54e-23	94.7
MS.gene68216.t1	MTR_3g075500	41.975	81	47	0	7	87	110	190	6.21e-12	62.8
MS.gene68216.t1	MTR_4g109540	47.500	80	42	0	7	86	43	122	4.77e-23	92.8
MS.gene68216.t1	MTR_3g025880	46.835	79	42	0	7	85	45	123	1.19e-22	94.0
MS.gene68216.t1	MTR_7g013730	44.578	83	46	0	7	89	41	123	1.94e-22	90.9
MS.gene68216.t1	MTR_4g109540	48.052	77	40	0	10	86	54	130	1.81e-21	89.0
MS.gene68216.t1	MTR_3g080130	46.341	82	44	0	7	88	37	118	3.63e-21	84.7
MS.gene68216.t1	MTR_4g104470	51.852	81	39	0	6	86	216	296	5.51e-21	87.8
MS.gene68216.t1	MTR_4g104470	38.235	102	63	0	5	106	121	222	6.81e-16	73.9
MS.gene68216.t1	MTR_1g064230	55.000	80	36	0	7	86	197	276	7.49e-21	87.4
MS.gene68216.t1	MTR_3g014000	46.939	98	40	3	8	98	36	128	4.35e-20	82.0
MS.gene68216.t1	MTR_3g075500	55.882	68	30	0	3	70	203	270	6.81e-19	81.6
MS.gene68216.t1	MTR_3g075500	41.975	81	47	0	7	87	110	190	6.99e-12	62.8
MS.gene68216.t1	MTR_7g099940	49.367	79	40	0	7	85	36	114	8.01e-19	79.0
MS.gene68216.t1	MTR_4g035310	45.263	95	44	3	7	95	17	109	1.73e-18	80.9
MS.gene68216.t1	MTR_4g035310	45.263	95	44	3	7	95	17	109	2.04e-18	80.5
MS.gene68216.t1	MTR_7g105260	43.210	81	46	0	4	84	30	110	2.69e-18	77.4
MS.gene68216.t1	MTR_3g084040	51.562	64	30	1	7	70	37	99	6.47e-18	75.5
MS.gene68216.t1	MTR_6g477870	43.750	80	45	0	7	86	96	175	1.58e-16	73.9
MS.gene68216.t1	MTR_1g045510	43.902	82	46	0	2	83	200	281	1.89e-16	75.5
MS.gene68216.t1	MTR_3g055990	39.241	79	46	1	7	85	7	83	3.28e-16	75.9
MS.gene68216.t1	MTR_3g116130	40.708	113	63	3	7	116	30	141	3.43e-16	74.7
MS.gene68216.t1	MTR_3g055990	39.241	79	46	1	7	85	7	83	3.62e-16	75.9
MS.gene68216.t1	MTR_8g024520	32.812	128	70	2	7	119	26	152	7.33e-16	73.6
MS.gene68216.t1	MTR_8g024520	32.812	128	70	2	7	119	26	152	7.49e-16	73.6
MS.gene68216.t1	MTR_3g092490	34.862	109	69	2	7	115	27	133	1.06e-15	71.6
MS.gene68216.t1	MTR_3g092490	28.916	166	106	2	7	161	27	191	1.13e-15	72.8
MS.gene68216.t1	MTR_3g116130	40.708	113	63	3	7	116	30	141	1.16e-15	73.9
MS.gene68216.t1	MTR_4g081520	43.820	89	50	0	1	89	46	134	1.22e-15	70.5
MS.gene68216.t1	MTR_3g092490	38.298	94	57	1	7	100	27	119	1.24e-15	72.8
MS.gene68216.t1	MTR_5g023060	33.333	171	106	5	8	171	133	302	1.40e-15	73.2
MS.gene68216.t1	MTR_7g013740	39.640	111	54	1	7	117	41	138	3.34e-15	69.3
MS.gene68216.t1	MTR_2g096560	39.024	82	50	0	7	88	8	89	3.60e-15	67.8
MS.gene68216.t1	MTR_5g097580	40.506	79	45	2	7	85	7	83	1.69e-14	70.9
MS.gene68216.t1	MTR_5g097580	33.010	103	64	2	7	109	107	204	3.21e-11	61.6
MS.gene68216.t1	MTR_3g065190	29.577	142	98	2	8	147	114	255	1.72e-14	70.9
MS.gene68216.t1	MTR_5g097580	40.506	79	45	2	7	85	7	83	1.75e-14	70.9
MS.gene68216.t1	MTR_5g097580	33.010	103	64	2	7	109	107	204	3.85e-11	61.2
MS.gene68216.t1	MTR_1g073690	35.443	79	49	1	7	85	7	83	3.97e-14	69.7
MS.gene68216.t1	MTR_1g073690	34.483	87	56	1	7	93	113	198	2.69e-13	67.4
MS.gene68216.t1	MTR_2g026180	36.709	79	48	1	7	85	5	81	7.33e-14	68.9
MS.gene68216.t1	MTR_5g064120	42.105	76	43	1	7	82	17	91	1.56e-13	67.4
MS.gene68216.t1	MTR_1g092860	41.026	78	46	0	7	84	193	270	1.79e-13	67.4
MS.gene68216.t1	MTR_7g117495	47.458	59	31	0	7	65	19	77	4.94e-13	65.5
MS.gene68216.t1	MTR_7g117495	47.458	59	31	0	7	65	19	77	5.41e-13	65.5
MS.gene68216.t1	MTR_3g029580	40.541	74	44	0	7	80	33	106	1.38e-12	62.4
MS.gene68216.t1	MTR_4g063560	41.096	73	43	0	7	79	34	106	1.73e-12	61.2
MS.gene68216.t1	MTR_6g477870	42.188	64	37	0	7	70	96	159	3.48e-12	62.0
MS.gene68216.t1	MTR_4g085540	44.444	72	39	1	8	79	124	194	6.03e-12	63.5
MS.gene68216.t1	MTR_4g117350	35.165	91	58	1	7	97	72	161	1.11e-11	60.5
MS.gene68216.t1	MTR_4g036415	43.056	72	41	0	8	79	453	524	2.01e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene68216.t1	AT4G39260	74.725	91	23	0	1	91	1	91	1.22e-46	148
MS.gene68216.t1	AT2G21660	79.012	81	17	0	3	83	5	85	7.43e-44	143
MS.gene68216.t1	AT2G21660	80.899	89	17	0	3	91	5	93	6.95e-43	141
MS.gene68216.t1	AT4G39260	72.289	83	23	0	1	83	1	83	1.80e-41	136
MS.gene68216.t1	AT4G39260	72.289	83	23	0	1	83	1	83	6.53e-41	136
MS.gene68216.t1	AT4G39260	70.588	68	20	0	1	68	1	68	2.41e-32	112
MS.gene68216.t1	AT3G26420	37.118	229	87	6	1	172	1	229	6.06e-28	105
MS.gene68216.t1	AT3G23830	56.627	83	36	0	7	89	36	118	7.66e-28	102
MS.gene68216.t1	AT3G23830	56.627	83	36	0	7	89	36	118	7.66e-28	102
MS.gene68216.t1	AT4G13850	54.217	83	38	0	7	89	36	118	3.43e-25	95.5
MS.gene68216.t1	AT4G13850	54.217	83	38	0	7	89	36	118	4.36e-25	95.5
MS.gene68216.t1	AT4G13850	54.217	83	38	0	7	89	36	118	4.96e-25	95.5
MS.gene68216.t1	AT4G13850	55.556	81	36	0	7	87	36	116	5.20e-25	94.4
MS.gene68216.t1	AT3G53460	51.807	83	40	0	7	89	258	340	9.04e-23	93.6
MS.gene68216.t1	AT3G53460	51.807	83	40	0	7	89	258	340	9.04e-23	93.6
MS.gene68216.t1	AT3G53460	51.807	83	40	0	7	89	258	340	9.04e-23	93.6
MS.gene68216.t1	AT2G37220	50.617	81	40	0	7	87	205	285	9.54e-23	92.4
MS.gene68216.t1	AT2G37220	43.421	76	43	0	5	80	90	165	3.29e-12	63.9
MS.gene68216.t1	AT3G53460	51.807	83	40	0	7	89	279	361	1.31e-22	93.2
MS.gene68216.t1	AT4G24770	51.220	82	40	0	6	87	244	325	5.60e-22	91.3
MS.gene68216.t1	AT4G24770	40.196	102	61	0	5	106	149	250	4.95e-16	74.7
MS.gene68216.t1	AT4G24770	51.220	82	40	0	6	87	244	325	5.60e-22	91.3
MS.gene68216.t1	AT4G24770	40.196	102	61	0	5	106	149	250	4.95e-16	74.7
MS.gene68216.t1	AT5G04280	45.161	93	51	0	7	99	8	100	2.66e-21	89.0
MS.gene68216.t1	AT5G06210	44.444	90	50	0	4	93	32	121	3.99e-20	82.4
MS.gene68216.t1	AT5G50250	50.000	80	40	0	6	85	207	286	6.02e-20	85.1
MS.gene68216.t1	AT5G50250	39.216	102	62	0	5	106	112	213	2.58e-15	72.4
MS.gene68216.t1	AT1G18630	50.000	78	39	0	7	84	37	114	8.29e-20	81.6
MS.gene68216.t1	AT2G21690	47.126	87	41	1	4	85	5	91	6.18e-19	78.6
MS.gene68216.t1	AT1G60650	42.683	82	47	0	4	85	10	91	1.46e-18	81.3
MS.gene68216.t1	AT1G60650	42.683	82	47	0	4	85	10	91	1.46e-18	81.3
MS.gene68216.t1	AT5G47320	49.351	77	39	0	7	83	32	108	4.48e-18	78.6
MS.gene68216.t1	AT2G46780	43.269	104	52	3	7	104	23	125	1.28e-17	79.3
MS.gene68216.t1	AT2G46780	43.269	104	52	3	7	104	23	125	1.28e-17	79.3
MS.gene68216.t1	AT5G61030	38.462	117	40	1	7	91	41	157	1.82e-17	78.6
MS.gene68216.t1	AT5G61030	38.462	117	40	1	7	91	41	157	1.82e-17	78.6
MS.gene68216.t1	AT2G37510	51.351	74	36	0	7	80	35	108	2.00e-17	75.1
MS.gene68216.t1	AT1G22910	44.681	94	46	2	7	95	14	106	2.59e-17	77.4
MS.gene68216.t1	AT1G22910	44.681	94	46	2	7	95	14	106	3.46e-17	76.6
MS.gene68216.t1	AT1G74230	53.571	112	51	1	7	118	35	145	1.52e-16	75.9
MS.gene68216.t1	AT3G07810	40.506	79	45	1	7	85	7	83	2.08e-16	76.6
MS.gene68216.t1	AT3G07810	40.506	79	45	1	7	85	7	83	2.14e-16	76.6
MS.gene68216.t1	AT1G22330	41.176	102	51	2	7	100	18	118	2.64e-16	75.1
MS.gene68216.t1	AT1G22910	44.681	94	46	2	7	95	14	106	2.84e-16	75.5
MS.gene68216.t1	AT2G33410	32.143	112	74	1	7	118	7	116	3.21e-16	75.9
MS.gene68216.t1	AT4G13860	45.205	73	40	0	7	79	4	76	5.43e-16	70.1
MS.gene68216.t1	AT1G20880	46.053	76	40	1	7	82	25	99	6.63e-16	73.9
MS.gene68216.t1	AT1G20880	46.053	76	40	1	7	82	25	99	6.63e-16	73.9
MS.gene68216.t1	AT1G20880	46.053	76	40	1	7	82	25	99	6.63e-16	73.9
MS.gene68216.t1	AT5G59865	46.835	79	42	0	9	87	29	107	1.03e-15	70.1
MS.gene68216.t1	AT1G33470	46.753	77	38	2	7	82	8	82	2.16e-15	72.0
MS.gene68216.t1	AT1G33470	46.753	77	38	2	7	82	8	82	2.49e-15	72.0
MS.gene68216.t1	AT1G33470	46.753	77	38	2	7	82	8	82	2.74e-15	71.6
MS.gene68216.t1	AT1G60000	45.122	82	45	0	2	83	173	254	2.91e-15	72.0
MS.gene68216.t1	AT1G76460	46.053	76	40	1	7	82	25	99	3.75e-15	72.0
MS.gene68216.t1	AT5G19960	37.037	108	66	2	8	115	9	114	4.19e-15	72.4
MS.gene68216.t1	AT3G08000	44.048	84	47	0	7	90	42	125	7.28e-15	68.6
MS.gene68216.t1	AT4G14300	37.975	79	47	1	7	85	7	83	8.77e-15	71.6
MS.gene68216.t1	AT4G14300	37.975	79	47	1	7	85	7	83	8.77e-15	71.6
MS.gene68216.t1	AT3G46020	39.286	84	50	1	1	83	1	84	1.07e-14	67.0
MS.gene68216.t1	AT5G55550	39.241	79	46	1	7	85	7	83	1.26e-14	71.2
MS.gene68216.t1	AT5G55550	39.241	79	46	1	7	85	7	83	1.26e-14	71.2
MS.gene68216.t1	AT5G55550	39.241	79	46	1	7	85	7	83	1.26e-14	71.2
MS.gene68216.t1	AT5G55550	39.241	79	46	1	7	85	7	83	1.26e-14	71.2
MS.gene68216.t1	AT5G55550	39.241	79	46	1	7	85	7	83	1.26e-14	71.2
MS.gene68216.t1	AT4G26650	37.975	79	47	1	7	85	13	89	1.53e-14	71.2
MS.gene68216.t1	AT4G26650	37.975	79	47	1	7	85	16	92	1.55e-14	71.2
MS.gene68216.t1	AT5G55550	39.241	79	46	1	7	85	7	83	1.65e-14	70.9
MS.gene68216.t1	AT5G55550	39.241	79	46	1	7	85	7	83	1.65e-14	70.9
MS.gene68216.t1	AT5G55550	39.241	79	46	1	7	85	7	83	1.65e-14	70.9
MS.gene68216.t1	AT5G55550	39.241	79	46	1	7	85	7	83	1.65e-14	70.9
MS.gene68216.t1	AT1G78260	43.421	76	42	1	7	82	18	92	3.09e-14	69.3
MS.gene68216.t1	AT1G78260	43.421	76	42	1	7	82	18	92	5.01e-14	68.6
MS.gene68216.t1	AT3G13224	34.568	81	53	0	7	87	110	190	1.01e-13	68.6
MS.gene68216.t1	AT3G13224	34.524	84	53	1	7	90	20	101	2.00e-12	64.7
MS.gene68216.t1	AT1G73530	36.250	80	51	0	7	86	78	157	2.04e-13	65.5
MS.gene68216.t1	AT2G23350	48.611	72	36	1	8	79	136	206	2.84e-13	67.4
MS.gene68216.t1	AT3G13224	34.568	81	53	0	7	87	101	181	3.07e-13	67.0
MS.gene68216.t1	AT3G13224	34.043	94	56	2	1	90	1	92	9.67e-13	65.5
MS.gene68216.t1	AT5G55550	40.278	72	41	1	7	78	7	76	3.16e-13	67.4
MS.gene68216.t1	AT5G55550	40.278	72	41	1	7	78	7	76	3.58e-13	67.0
MS.gene68216.t1	AT2G27330	29.670	91	64	0	9	99	24	114	4.19e-13	63.2
MS.gene68216.t1	AT5G53720	40.404	99	53	3	5	103	8	100	4.55e-13	62.8
MS.gene68216.t1	AT1G01080	41.463	82	45	2	1	81	207	286	5.92e-13	65.9
MS.gene68216.t1	AT1G01080	41.463	82	45	2	1	81	208	287	6.21e-13	65.9
MS.gene68216.t1	AT3G20930	37.500	80	50	0	7	86	283	362	9.53e-13	65.9
MS.gene68216.t1	AT5G51300	42.667	75	43	0	5	79	479	553	9.82e-13	65.9
MS.gene68216.t1	AT5G51300	42.667	75	43	0	5	79	479	553	9.82e-13	65.9
MS.gene68216.t1	AT5G51300	42.667	75	43	0	5	79	479	553	9.82e-13	65.9
MS.gene68216.t1	AT5G47620	35.443	79	49	1	7	85	7	83	1.05e-12	65.9
MS.gene68216.t1	AT5G47620	35.443	79	49	1	7	85	7	83	1.05e-12	65.9
MS.gene68216.t1	AT5G47620	35.443	79	49	1	7	85	7	83	1.05e-12	65.9
MS.gene68216.t1	AT3G54770	42.105	76	43	1	7	82	18	92	1.10e-12	64.7
MS.gene68216.t1	AT3G54770	42.105	76	43	1	7	82	18	92	1.26e-12	64.7
MS.gene68216.t1	AT1G17640	38.750	80	47	1	7	86	67	144	2.72e-12	64.3
MS.gene68216.t1	AT1G17640	32.990	97	64	1	7	103	158	253	3.43e-12	64.3
MS.gene68216.t1	AT1G17640	38.750	80	47	1	7	86	67	144	2.72e-12	64.3
MS.gene68216.t1	AT1G17640	32.990	97	64	1	7	103	158	253	3.43e-12	64.3
MS.gene68216.t1	AT1G17640	38.750	80	47	1	7	86	67	144	2.72e-12	64.3
MS.gene68216.t1	AT1G17640	32.990	97	64	1	7	103	158	253	3.43e-12	64.3
MS.gene68216.t1	AT5G54580	44.048	84	47	0	2	85	52	135	2.81e-12	62.0
MS.gene68216.t1	AT4G13860	46.667	60	32	0	21	80	1	60	6.07e-12	58.9
MS.gene68216.t1	AT3G52150	44.156	77	43	0	6	82	177	253	8.07e-12	62.4
MS.gene68216.t1	AT3G52150	44.156	77	43	0	6	82	177	253	8.07e-12	62.4
MS.gene68216.t1	AT2G37510	38.384	99	36	1	7	80	35	133	8.65e-12	60.8
MS.gene68216.t1	AT5G53680	49.254	67	27	2	7	66	14	80	9.83e-12	60.8
MS.gene68216.t1	AT5G47620	36.111	72	44	1	7	78	7	76	1.31e-11	62.8
MS.gene68216.t1	AT2G18510	32.759	116	76	2	8	121	114	229	1.56e-11	62.4
MS.gene68216.t1	AT3G14100	41.026	78	46	0	5	82	143	220	1.56e-11	62.4
MS.gene68216.t1	AT1G54080	41.026	78	46	0	5	82	147	224	3.83e-11	61.2
MS.gene68216.t1	AT2G22100	53.061	49	23	0	8	56	165	213	6.63e-11	60.5

Find 87 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTGGATATGGTGGAGGAGG+CGG	0.278119	3.2:-92106157	MS.gene68216:CDS
GGTGGATATGGTGGAGGTGG+CGG	0.285692	3.2:-92106214	MS.gene68216:CDS
GTGGATATGGTGGAGGTGGC+GGG	0.285761	3.2:-92106213	MS.gene68216:CDS
CGTGATGGTGGTGGTGGATA+TGG	0.293140	3.2:-92106106	MS.gene68216:CDS
ACTGGAAGATCAAGAGGATT+TGG	0.294131	3.2:-92106424	MS.gene68216:CDS
GTGGATATGGTGGAGGAGGC+GGG	0.301740	3.2:-92106156	MS.gene68216:CDS
CGCGGAGGTGGTGGTGGATA+TGG	0.305180	3.2:-92106262	MS.gene68216:CDS
CGTGGAGGAGGTGGTGGATA+TGG	0.306969	3.2:-92106169	MS.gene68216:CDS
CGTCGTGGAGGTGGTGGATA+TGG	0.325761	3.2:-92106226	MS.gene68216:CDS
AGTGGCGGAGGCGGTGGTGG+TGG	0.340361	3.2:-92106292	MS.gene68216:CDS
GTTATCACCGATCGTGAAAC+TGG	0.362798	3.2:-92106442	MS.gene68216:CDS
TGGATATGGTGGAGGTGGCG+GGG	0.366937	3.2:-92106212	MS.gene68216:CDS
GGATATGGTGGAGGTGGCGG+GGG	0.367929	3.2:-92106211	MS.gene68216:CDS
TGGATATGGTGGAGGAGGCG+GGG	0.372204	3.2:-92106155	MS.gene68216:CDS
ATCACCGCCTCTCGAGTATC+TGG	0.373006	3.2:+92106057	None:intergenic
GGCGGAGGCGGTGGTGGTGG+TGG	0.379371	3.2:-92106289	MS.gene68216:CDS
GGCAGTGGCGGAGGCGGTGG+TGG	0.383736	3.2:-92106295	MS.gene68216:CDS
GGATATGGTGGAGGAGGCGG+GGG	0.387784	3.2:-92106154	MS.gene68216:CDS
CGTGGCAGTGGCGGAGGCGG+TGG	0.400483	3.2:-92106298	MS.gene68216:CDS
GGATACCGCGGAGGTGGTGG+TGG	0.412147	3.2:-92106268	MS.gene68216:CDS
GGTGGATACCGCGGAGGTGG+TGG	0.418043	3.2:-92106271	MS.gene68216:CDS
GGTTACGGAGGTGGTGGTGG+CGG	0.440050	3.2:-92106133	MS.gene68216:CDS
GGTGGTGGATATGGTGGAGG+TGG	0.440960	3.2:-92106217	MS.gene68216:CDS
GGTGGCGAACGTCGTGGAGG+TGG	0.444469	3.2:-92106235	MS.gene68216:CDS
GGTGGTGACCGTCGTGGAGG+AGG	0.444501	3.2:-92106181	MS.gene68216:CDS
GACCGTCGTGGAGGAGGTGG+TGG	0.446949	3.2:-92106175	MS.gene68216:CDS
GGTGGTGGATATGGTGGAGG+AGG	0.448331	3.2:-92106160	MS.gene68216:CDS
GGTGGTGGATATGGCGGAGG+TGG	0.452527	3.2:-92106253	MS.gene68216:CDS
CAGAGTCGTGGCAGTGGCGG+AGG	0.453358	3.2:-92106304	MS.gene68216:CDS
GGTGGTGGATACCGCGGAGG+TGG	0.455995	3.2:-92106274	MS.gene68216:CDS
AGTCGTGGCAGTGGCGGAGG+CGG	0.456959	3.2:-92106301	MS.gene68216:CDS
GTGGCTCTGACAGTAGAAAC+TGG	0.459380	3.2:-92106030	MS.gene68216:CDS
GTTGAATACCGATGCTTTGT+TGG	0.464495	3.2:-92106826	MS.gene68216:CDS
GGTGACCGTCGTGGAGGAGG+TGG	0.465249	3.2:-92106178	MS.gene68216:CDS
CTTTGTTGGTGGACTTGCAT+GGG	0.465470	3.2:-92106812	MS.gene68216:CDS
GGCGAACGTCGTGGAGGTGG+TGG	0.469494	3.2:-92106232	MS.gene68216:CDS
GATGGTGGTGGTGGATATGG+TGG	0.469678	3.2:-92106103	MS.gene68216:CDS
GGTGGTGGATATGGTGGAGG+TGG	0.471808	3.2:-92106097	MS.gene68216:CDS
GGATATGGTGGAGGTGGCTA+CGG	0.473053	3.2:-92106091	MS.gene68216:CDS
GGAGGAGGTGGTGGATATGG+TGG	0.473329	3.2:-92106166	MS.gene68216:CDS
GGAGGTGGTGGATATGGTGG+AGG	0.473487	3.2:-92106163	MS.gene68216:CDS
CTCAAGAGCTTCGTTGTCAG+TGG	0.495714	3.2:+92106789	None:intergenic
GGTGGTGGTGGATATGGCGG+AGG	0.496613	3.2:-92106256	MS.gene68216:CDS
GGTGGTGGTGGATATGGTGG+AGG	0.497470	3.2:-92106100	MS.gene68216:CDS
GCGATGAAAGATGCGATTGA+AGG	0.503882	3.2:-92106376	MS.gene68216:CDS
ATGAACGGCCAAGATCTTGA+TGG	0.505893	3.2:-92106352	MS.gene68216:CDS
GGTGGAGGTGGCTACGGAGA+TGG	0.509409	3.2:-92106085	MS.gene68216:CDS
TGTCACATTCTCTACGGAGC+AGG	0.509697	3.2:-92106398	MS.gene68216:CDS
GGACGTCGTGATGGTGGTGG+TGG	0.511175	3.2:-92106112	MS.gene68216:CDS
CGTGGAGGTGGTGGATATGG+TGG	0.512534	3.2:-92106223	MS.gene68216:CDS
GGTGGTGGCGGACGTCGTGA+TGG	0.514583	3.2:-92106121	MS.gene68216:CDS
GCTTTGTTGGTGGACTTGCA+TGG	0.519425	3.2:-92106813	MS.gene68216:CDS
TGGATTTGTCACATTCTCTA+CGG	0.520808	3.2:-92106404	MS.gene68216:CDS
TGATCTTCCAGTTTCACGAT+CGG	0.521619	3.2:+92106435	None:intergenic
GAGGCTCAGAGTCGTGGCAG+TGG	0.522443	3.2:-92106310	MS.gene68216:CDS
TGTTACGTCCATCAAGATCT+TGG	0.527371	3.2:+92106344	None:intergenic
TCGAGAGGCGGTGATGGCGG+TGG	0.528930	3.2:-92106049	MS.gene68216:CDS
GGAGGTGGTGGATATGGTGG+AGG	0.534655	3.2:-92106220	MS.gene68216:CDS
GGCGGACGTCGTGATGGTGG+TGG	0.542567	3.2:-92106115	MS.gene68216:CDS
CGATGAAAGATGCGATTGAA+GGG	0.542649	3.2:-92106375	MS.gene68216:CDS
TGGTGAAATCGTCGAATCGA+AGG	0.542807	3.2:-92106749	MS.gene68216:intron
GCTCAGAGTCGTGGCAGTGG+CGG	0.545233	3.2:-92106307	MS.gene68216:CDS
CGATTCGACGATTTCACCAA+AGG	0.547099	3.2:+92106753	None:intergenic
GGTGGCGGACGTCGTGATGG+TGG	0.554420	3.2:-92106118	MS.gene68216:CDS
AGATACTCGAGAGGCGGTGA+TGG	0.555850	3.2:-92106055	MS.gene68216:CDS
GGAGGTGGCTACGGAGATGG+TGG	0.556120	3.2:-92106082	MS.gene68216:CDS
CGTGAAACTGGAAGATCAAG+AGG	0.575579	3.2:-92106430	MS.gene68216:CDS
GAATACCGATGCTTTGTTGG+TGG	0.577401	3.2:-92106823	MS.gene68216:CDS
TACTCGAGAGGCGGTGATGG+CGG	0.579779	3.2:-92106052	MS.gene68216:CDS
ACGTAACATCACTGTGAATG+AGG	0.581570	3.2:-92106329	MS.gene68216:CDS
TCGGTATTCAACTTCCGCCA+TGG	0.595134	3.2:+92106837	None:intergenic
GGAGGTGGTGGTGGATATGG+CGG	0.597374	3.2:-92106259	MS.gene68216:CDS
TATGGAGGTGGTGACCGTCG+TGG	0.615014	3.2:-92106187	MS.gene68216:CDS
CTCTGACAGTAGAAACTGGA+GGG	0.619176	3.2:-92106026	MS.gene68216:CDS
GATGCGATTGAAGGGATGAA+CGG	0.619661	3.2:-92106367	MS.gene68216:CDS
GTGAATGAGGCTCAGAGTCG+TGG	0.619893	3.2:-92106316	MS.gene68216:CDS
GGTGGTGGTGGTGGATACCG+CGG	0.621729	3.2:-92106280	MS.gene68216:CDS
GGCGGAGGTGGCGAACGTCG+TGG	0.626375	3.2:-92106241	MS.gene68216:CDS
CAAGTCCACCAACAAAGCAT+CGG	0.636350	3.2:+92106818	None:intergenic
GGCTCCAGATACTCGAGAGG+CGG	0.636811	3.2:-92106061	MS.gene68216:CDS
GGAGGTGGCGAACGTCGTGG+AGG	0.643779	3.2:-92106238	MS.gene68216:CDS
GGAGGTGGTGACCGTCGTGG+AGG	0.653524	3.2:-92106184	MS.gene68216:CDS
GGTGGTGGTGGATACCGCGG+AGG	0.661432	3.2:-92106277	MS.gene68216:CDS
ATCCACCACCTCCTCCACGA+CGG	0.697052	3.2:+92106173	None:intergenic
CATATCCACCACCACCTCCG+CGG	0.713479	3.2:+92106263	None:intergenic
GCTCTGACAGTAGAAACTGG+AGG	0.714944	3.2:-92106027	MS.gene68216:CDS
GGTGGCTCCAGATACTCGAG+AGG	0.728248	3.2:-92106064	MS.gene68216:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTATTAATCACTTCAAAT+CGG	-	chr3.2:92106273-92106292	MS.gene68216:CDS	15.0%
!!!	ACGATTCTTTGTTTTTGTTT+AGG	-	chr3.2:92106392-92106411	MS.gene68216:CDS	25.0%
	TGTGTAAATTGAAGATCGAT+CGG	-	chr3.2:92106314-92106333	MS.gene68216:CDS	30.0%
	TGGATTTGTCACATTCTCTA+CGG	-	chr3.2:92106452-92106471	MS.gene68216:intron	35.0%
	ACAGAACAGAACAGAACAGA+AGG	+	chr3.2:92106191-92106210	None:intergenic	40.0%
	ACGTAACATCACTGTGAATG+AGG	-	chr3.2:92106527-92106546	MS.gene68216:intron	40.0%
	ACTGGAAGATCAAGAGGATT+TGG	-	chr3.2:92106432-92106451	MS.gene68216:CDS	40.0%
	ATCGGAAATCTGACACGATT+CGG	-	chr3.2:92106146-92106165	MS.gene68216:CDS	40.0%
	CGATGAAAGATGCGATTGAA+GGG	-	chr3.2:92106481-92106500	MS.gene68216:intron	40.0%
	GTTGAATACCGATGCTTTGT+TGG	-	chr3.2:92106030-92106049	MS.gene68216:CDS	40.0%
	TGATCTTCCAGTTTCACGAT+CGG	+	chr3.2:92106424-92106443	None:intergenic	40.0%
	TGTTACGTCCATCAAGATCT+TGG	+	chr3.2:92106515-92106534	None:intergenic	40.0%
	TTTCCGATCTCAGCAACAAA+CGG	+	chr3.2:92106134-92106153	None:intergenic	40.0%
!	AATCTGACACGATTCGGATT+TGG	-	chr3.2:92106152-92106171	MS.gene68216:CDS	40.0%
!!!	GAGAAAGCGTTTTCTTCCTT+TGG	-	chr3.2:92106087-92106106	MS.gene68216:CDS	40.0%
	ATGAACGGCCAAGATCTTGA+TGG	-	chr3.2:92106504-92106523	MS.gene68216:intron	45.0%
	CAAGTCCACCAACAAAGCAT+CGG	+	chr3.2:92106041-92106060	None:intergenic	45.0%
	CGATTCGACGATTTCACCAA+AGG	+	chr3.2:92106106-92106125	None:intergenic	45.0%
	CGTGAAACTGGAAGATCAAG+AGG	-	chr3.2:92106426-92106445	MS.gene68216:CDS	45.0%
	CTCTGACAGTAGAAACTGGA+GGG	-	chr3.2:92106830-92106849	MS.gene68216:CDS	45.0%
	GATGCGATTGAAGGGATGAA+CGG	-	chr3.2:92106489-92106508	MS.gene68216:intron	45.0%
	GCGATGAAAGATGCGATTGA+AGG	-	chr3.2:92106480-92106499	MS.gene68216:intron	45.0%
	GTTATCACCGATCGTGAAAC+TGG	-	chr3.2:92106414-92106433	MS.gene68216:CDS	45.0%
	TGGTGAAATCGTCGAATCGA+AGG	-	chr3.2:92106107-92106126	MS.gene68216:CDS	45.0%
!	GAATACCGATGCTTTGTTGG+TGG	-	chr3.2:92106033-92106052	MS.gene68216:CDS	45.0%
!!	CTTTGTTGGTGGACTTGCAT+GGG	-	chr3.2:92106044-92106063	MS.gene68216:CDS	45.0%
	CTCAAGAGCTTCGTTGTCAG+TGG	+	chr3.2:92106070-92106089	None:intergenic	50.0%
	GCTCTGACAGTAGAAACTGG+AGG	-	chr3.2:92106829-92106848	MS.gene68216:CDS	50.0%
	TGTCACATTCTCTACGGAGC+AGG	-	chr3.2:92106458-92106477	MS.gene68216:intron	50.0%
!	GGTCCGTTTGTTGCTGAGAT+CGG	-	chr3.2:92106128-92106147	MS.gene68216:CDS	50.0%
!!	GCTTTGTTGGTGGACTTGCA+TGG	-	chr3.2:92106043-92106062	MS.gene68216:CDS	50.0%
!!	GTGGCTCTGACAGTAGAAAC+TGG	-	chr3.2:92106826-92106845	MS.gene68216:CDS	50.0%
	AGATACTCGAGAGGCGGTGA+TGG	-	chr3.2:92106801-92106820	MS.gene68216:CDS	55.0%
	ATCACCGCCTCTCGAGTATC+TGG	+	chr3.2:92106802-92106821	None:intergenic	55.0%
	GTGAATGAGGCTCAGAGTCG+TGG	-	chr3.2:92106540-92106559	MS.gene68216:intron	55.0%
!	GGATATGGTGGAGGTGGCTA+CGG	-	chr3.2:92106765-92106784	MS.gene68216:CDS	55.0%
!!	CGTGATGGTGGTGGTGGATA+TGG	-	chr3.2:92106750-92106769	MS.gene68216:CDS	55.0%
!!	GATGGTGGTGGTGGATATGG+TGG	-	chr3.2:92106753-92106772	MS.gene68216:CDS	55.0%
	ATCCACCACCTCCTCCACGA+CGG	+	chr3.2:92106686-92106705	None:intergenic	60.0%
	CATATCCACCACCACCTCCG+CGG	+	chr3.2:92106596-92106615	None:intergenic	60.0%
	GGCTCCAGATACTCGAGAGG+CGG	-	chr3.2:92106795-92106814	MS.gene68216:CDS	60.0%
	GGTGGCTCCAGATACTCGAG+AGG	-	chr3.2:92106792-92106811	MS.gene68216:CDS	60.0%
	TATGGAGGTGGTGACCGTCG+TGG	-	chr3.2:92106669-92106688	MS.gene68216:intron	60.0%
!	GGTGGATATGGTGGAGGAGG+CGG	-	chr3.2:92106699-92106718	MS.gene68216:intron	60.0%
!	GGTGGATATGGTGGAGGTGG+CGG	-	chr3.2:92106642-92106661	MS.gene68216:intron	60.0%
!	GTGGATATGGTGGAGGAGGC+GGG	-	chr3.2:92106700-92106719	MS.gene68216:intron	60.0%
!	GTGGATATGGTGGAGGTGGC+GGG	-	chr3.2:92106643-92106662	MS.gene68216:intron	60.0%
!	TACTCGAGAGGCGGTGATGG+CGG	-	chr3.2:92106804-92106823	MS.gene68216:CDS	60.0%
!	TGGATATGGTGGAGGAGGCG+GGG	-	chr3.2:92106701-92106720	MS.gene68216:intron	60.0%
!	TGGATATGGTGGAGGTGGCG+GGG	-	chr3.2:92106644-92106663	MS.gene68216:intron	60.0%
!!	CGTCGTGGAGGTGGTGGATA+TGG	-	chr3.2:92106630-92106649	MS.gene68216:intron	60.0%
!!	CGTGGAGGAGGTGGTGGATA+TGG	-	chr3.2:92106687-92106706	MS.gene68216:intron	60.0%
!!	CGTGGAGGTGGTGGATATGG+TGG	-	chr3.2:92106633-92106652	MS.gene68216:intron	60.0%
!!	GGAGGAGGTGGTGGATATGG+TGG	-	chr3.2:92106690-92106709	MS.gene68216:intron	60.0%
!!	GGAGGTGGTGGATATGGTGG+AGG	-	chr3.2:92106636-92106655	MS.gene68216:intron	60.0%
!!	GGAGGTGGTGGTGGATATGG+CGG	-	chr3.2:92106597-92106616	MS.gene68216:intron	60.0%
!!	GGTGGTGGATATGGTGGAGG+AGG	-	chr3.2:92106639-92106658	MS.gene68216:intron	60.0%
!!	GGTGGTGGATATGGTGGAGG+TGG	-	chr3.2:92106639-92106658	MS.gene68216:intron	60.0%
!!	GGTGGTGGTGGATATGGTGG+AGG	-	chr3.2:92106756-92106775	MS.gene68216:CDS	60.0%
	GAGGCTCAGAGTCGTGGCAG+TGG	-	chr3.2:92106546-92106565	MS.gene68216:intron	65.0%
	GCTCAGAGTCGTGGCAGTGG+CGG	-	chr3.2:92106549-92106568	MS.gene68216:intron	65.0%
	GGAGGTGGCTACGGAGATGG+TGG	-	chr3.2:92106774-92106793	MS.gene68216:CDS	65.0%
	GGTGGAGGTGGCTACGGAGA+TGG	-	chr3.2:92106771-92106790	MS.gene68216:CDS	65.0%
!	GGATATGGTGGAGGAGGCGG+GGG	-	chr3.2:92106702-92106721	MS.gene68216:intron	65.0%
!	GGATATGGTGGAGGTGGCGG+GGG	-	chr3.2:92106645-92106664	MS.gene68216:intron	65.0%
!!	CGCGGAGGTGGTGGTGGATA+TGG	-	chr3.2:92106594-92106613	MS.gene68216:intron	65.0%
!!	GGACGTCGTGATGGTGGTGG+TGG	-	chr3.2:92106744-92106763	MS.gene68216:intron	65.0%
!!	GGTGGTGGATATGGCGGAGG+TGG	-	chr3.2:92106603-92106622	MS.gene68216:intron	65.0%
!!	GGTGGTGGTGGATATGGCGG+AGG	-	chr3.2:92106600-92106619	MS.gene68216:intron	65.0%
!!	GGTGGTGGTGGTGGATACCG+CGG	-	chr3.2:92106576-92106595	MS.gene68216:intron	65.0%
!!	GGTTACGGAGGTGGTGGTGG+CGG	-	chr3.2:92106723-92106742	MS.gene68216:intron	65.0%
	CAGAGTCGTGGCAGTGGCGG+AGG	-	chr3.2:92106552-92106571	MS.gene68216:intron	70.0%
	GACCGTCGTGGAGGAGGTGG+TGG	-	chr3.2:92106681-92106700	MS.gene68216:intron	70.0%
	GGAGGTGGCGAACGTCGTGG+AGG	-	chr3.2:92106618-92106637	MS.gene68216:intron	70.0%
	GGAGGTGGTGACCGTCGTGG+AGG	-	chr3.2:92106672-92106691	MS.gene68216:intron	70.0%
	GGCGAACGTCGTGGAGGTGG+TGG	-	chr3.2:92106624-92106643	MS.gene68216:intron	70.0%
	GGTGACCGTCGTGGAGGAGG+TGG	-	chr3.2:92106678-92106697	MS.gene68216:intron	70.0%
	GGTGGAGGAGGCGGGGGTTA+CGG	-	chr3.2:92106708-92106727	MS.gene68216:intron	70.0%
	GGTGGATACCGCGGAGGTGG+TGG	-	chr3.2:92106585-92106604	MS.gene68216:intron	70.0%
	GGTGGCGAACGTCGTGGAGG+TGG	-	chr3.2:92106621-92106640	MS.gene68216:intron	70.0%
	GGTGGTGACCGTCGTGGAGG+AGG	-	chr3.2:92106675-92106694	MS.gene68216:intron	70.0%
!	AGTCGTGGCAGTGGCGGAGG+CGG	-	chr3.2:92106555-92106574	MS.gene68216:intron	70.0%
!	GGATACCGCGGAGGTGGTGG+TGG	-	chr3.2:92106588-92106607	MS.gene68216:intron	70.0%
!	GGGGGTTACGGAGGTGGTGG+TGG	-	chr3.2:92106720-92106739	MS.gene68216:intron	70.0%
!!	GGAGGTGGCGGGGGTTATGG+AGG	-	chr3.2:92106654-92106673	MS.gene68216:intron	70.0%
!!	GGCGGACGTCGTGATGGTGG+TGG	-	chr3.2:92106741-92106760	MS.gene68216:intron	70.0%
!!	GGTGGAGGTGGCGGGGGTTA+TGG	-	chr3.2:92106651-92106670	MS.gene68216:intron	70.0%
!!	GGTGGCGGACGTCGTGATGG+TGG	-	chr3.2:92106738-92106757	MS.gene68216:intron	70.0%
!!	GGTGGCGGGGGTTATGGAGG+TGG	-	chr3.2:92106657-92106676	MS.gene68216:intron	70.0%
!!	GGTGGTGGATACCGCGGAGG+TGG	-	chr3.2:92106582-92106601	MS.gene68216:intron	70.0%
!!	GGTGGTGGCGGACGTCGTGA+TGG	-	chr3.2:92106735-92106754	MS.gene68216:intron	70.0%
!!	GGTGGTGGTGGATACCGCGG+AGG	-	chr3.2:92106579-92106598	MS.gene68216:intron	70.0%
!!	TCGAGAGGCGGTGATGGCGG+TGG	-	chr3.2:92106807-92106826	MS.gene68216:CDS	70.0%
	GGAGGAGGCGGGGGTTACGG+AGG	-	chr3.2:92106711-92106730	MS.gene68216:intron	75.0%
	GGAGGCGGGGGTTACGGAGG+TGG	-	chr3.2:92106714-92106733	MS.gene68216:intron	75.0%
	GGCGGAGGTGGCGAACGTCG+TGG	-	chr3.2:92106615-92106634	MS.gene68216:intron	75.0%
	GGCGGGGGTTACGGAGGTGG+TGG	-	chr3.2:92106717-92106736	MS.gene68216:intron	75.0%
!	AGTGGCGGAGGCGGTGGTGG+TGG	-	chr3.2:92106564-92106583	MS.gene68216:intron	75.0%
!!	CGTGGCAGTGGCGGAGGCGG+TGG	-	chr3.2:92106558-92106577	MS.gene68216:intron	80.0%
!!	GGCAGTGGCGGAGGCGGTGG+TGG	-	chr3.2:92106561-92106580	MS.gene68216:intron	80.0%
!!!	GGCGGAGGCGGTGGTGGTGG+TGG	-	chr3.2:92106567-92106586	MS.gene68216:intron	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	92106021	92106857	92106021	ID=MS.gene68216
chr3.2	mRNA	92106021	92106857	92106021	ID=MS.gene68216.t1;Parent=MS.gene68216
chr3.2	exon	92106750	92106857	92106750	ID=MS.gene68216.t1.exon1;Parent=MS.gene68216.t1
chr3.2	CDS	92106750	92106857	92106750	ID=cds.MS.gene68216.t1;Parent=MS.gene68216.t1
chr3.2	exon	92106021	92106464	92106021	ID=MS.gene68216.t1.exon2;Parent=MS.gene68216.t1
chr3.2	CDS	92106021	92106464	92106021	ID=cds.MS.gene68216.t1;Parent=MS.gene68216.t1

Gene Sequence

>MS.gene68216

ATGGCGGAAGTTGAATACCGATGCTTTGTTGGTGGACTTGCATGGGCCACTGACAACGAAGCTCTTGAGAAAGCGTTTTCTTCCTTTGGTGAAATCGTCGAATCGAAGGTCCGTTTGTTGCTGAGATCGGAAATCTGACACGATTCGGATTTGGATTTTCGTGATCTCCTTCTGTTCTGTTCTGTTCTGTGTTGTTACTCTCTGATTACTTCTTCATCTTTGATACTCATCTGTTACTCTATAATGATTATCTTTTATTAATCACTTCAAATCGGTACGTTCATCTTCAATTTTGTGTAAATTGAAGATCGATCGGTTACTATCATTTTTTTTTATTTTTATAATTTATGCGAAATTGAGCTAGATCTGATACGATTCTTTGTTTTTGTTTAGGTTATCACCGATCGTGAAACTGGAAGATCAAGAGGATTTGGATTTGTCACATTCTCTACGGAGCAGGCGATGAAAGATGCGATTGAAGGGATGAACGGCCAAGATCTTGATGGACGTAACATCACTGTGAATGAGGCTCAGAGTCGTGGCAGTGGCGGAGGCGGTGGTGGTGGTGGATACCGCGGAGGTGGTGGTGGATATGGCGGAGGTGGCGAACGTCGTGGAGGTGGTGGATATGGTGGAGGTGGCGGGGGTTATGGAGGTGGTGACCGTCGTGGAGGAGGTGGTGGATATGGTGGAGGAGGCGGGGGTTACGGAGGTGGTGGTGGCGGACGTCGTGATGGTGGTGGTGGATATGGTGGAGGTGGCTACGGAGATGGTGGCTCCAGATACTCGAGAGGCGGTGATGGCGGTGGCTCTGACAGTAGAAACTGGAGGGAATAA

Protein sequence

>MS.gene68216.t1

MAEVEYRCFVGGLAWATDNEALEKAFSSFGEIVESKVITDRETGRSRGFGFVTFSTEQAMKDAIEGMNGQDLDGRNITVNEAQSRGSGGGGGGGGYRGGGGGYGGGGERRGGGGYGGGGGGYGGGDRRGGGGGYGGGGGGYGGGGGGRRDGGGGYGGGGYGDGGSRYSRGGDGGGSDSRNWRE