AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72653


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72653.t1 MTR_5g024180 96.825 126 4 0 1 126 1 126 4.02e-83 240
MS.gene72653.t1 MTR_8g073345 66.935 124 38 2 1 123 1 122 1.11e-50 158
MS.gene72653.t1 MTR_8g073345 66.935 124 38 2 1 123 1 122 1.62e-50 158
MS.gene72653.t1 MTR_5g024180 94.048 84 2 1 43 123 3 86 2.81e-48 151
MS.gene72653.t1 MTR_3g115580 60.952 105 33 2 6 105 13 114 1.59e-34 118
MS.gene72653.t1 MTR_7g068280 67.568 74 24 0 32 105 47 120 1.27e-29 105
MS.gene72653.t1 MTR_7g082820 64.557 79 26 2 28 105 4 81 1.43e-27 100
MS.gene72653.t1 MTR_1g115220 47.423 97 49 1 32 128 98 192 9.80e-25 94.7
MS.gene72653.t1 MTR_4g094622 38.272 81 50 0 25 105 53 133 6.38e-15 67.8
MS.gene72653.t1 MTR_0405s0060 94.118 34 2 0 82 115 74 107 2.32e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72653.t1 AT1G20693 63.810 105 34 2 18 122 23 123 3.11e-36 122
MS.gene72653.t1 AT1G20693 63.810 105 34 2 18 122 23 123 4.12e-36 121
MS.gene72653.t1 AT1G20693 63.810 105 34 2 18 122 23 123 4.24e-36 121
MS.gene72653.t1 AT1G20696 65.591 93 31 1 30 122 29 120 4.41e-35 119
MS.gene72653.t1 AT2G17560 48.462 130 64 1 6 135 8 134 8.77e-35 118
MS.gene72653.t1 AT2G17560 48.462 130 64 1 6 135 8 134 8.77e-35 118
MS.gene72653.t1 AT2G17560 51.695 118 54 1 6 123 8 122 1.53e-34 117
MS.gene72653.t1 AT1G20696 72.368 76 21 0 30 105 29 104 2.23e-34 117
MS.gene72653.t1 AT1G20696 72.368 76 21 0 30 105 29 104 4.29e-34 116
MS.gene72653.t1 AT3G51880 50.355 141 54 5 9 141 18 150 1.47e-31 111
MS.gene72653.t1 AT3G51880 53.226 124 43 4 9 124 18 134 3.71e-31 109
MS.gene72653.t1 AT3G51880 53.226 124 43 4 9 124 18 134 7.05e-31 109
MS.gene72653.t1 AT3G51880 53.226 124 43 4 9 124 18 134 7.05e-31 109
MS.gene72653.t1 AT3G51880 53.226 124 43 4 9 124 18 134 7.05e-31 109
MS.gene72653.t1 AT4G35570 45.763 118 58 2 7 122 9 122 1.31e-29 104
MS.gene72653.t1 AT4G35570 45.763 118 58 2 7 122 9 122 1.31e-29 104
MS.gene72653.t1 AT1G20696 48.980 98 34 2 44 125 1 98 1.01e-21 83.6
MS.gene72653.t1 AT1G20696 55.696 79 34 1 44 122 1 78 1.25e-21 83.6
MS.gene72653.t1 AT5G23420 49.275 69 32 2 34 101 113 179 1.34e-13 65.9
MS.gene72653.t1 AT2G34450 35.714 70 45 0 36 105 63 132 9.14e-13 62.0
MS.gene72653.t1 AT2G34450 35.714 70 45 0 36 105 63 132 9.14e-13 62.0
MS.gene72653.t1 AT2G34450 35.714 70 45 0 36 105 63 132 9.14e-13 62.0
MS.gene72653.t1 AT4G23800 49.315 73 34 1 32 104 251 320 1.47e-12 63.9
MS.gene72653.t1 AT4G23800 41.892 74 42 1 32 105 375 447 1.10e-11 61.6
MS.gene72653.t1 AT4G23800 49.315 73 34 1 32 104 251 320 1.61e-12 63.9
MS.gene72653.t1 AT4G23800 41.892 74 42 1 32 105 369 441 1.15e-11 61.6
MS.gene72653.t1 AT2G34450 36.620 71 44 1 36 105 63 133 2.94e-12 60.8
MS.gene72653.t1 AT4G11080 46.575 73 36 1 32 104 242 311 1.65e-11 60.8

Find 36 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAATGCGTGCTTACAATA+TGG 0.281097 5.4:+15577129 MS.gene72653:CDS
GGAGGCCTCTTAGGCTTGTT+AGG 0.297257 5.4:-15576540 None:intergenic
AAAGCACTTGGAGGCCTCTT+AGG 0.299005 5.4:-15576549 None:intergenic
TTGAATTAGGTTGGGAAAGC+TGG 0.350890 5.4:+15576925 MS.gene72653:intron
AAGCGCAAGGGCGCTGGTAC+TGG 0.369054 5.4:+15576489 MS.gene72653:CDS
AGGAGGATGAGGAGTAAAGT+TGG 0.417672 5.4:+15577428 MS.gene72653:CDS
TTATCAGCCTTAGCATTGAA+TGG 0.428927 5.4:-15577085 None:intergenic
ACAGCAACGGACTTGTTGCT+TGG 0.433440 5.4:-15576815 None:intergenic
TTAGCATCGGAAACAGCTTT+CGG 0.437399 5.4:-15576350 None:intergenic
ATGCTAAACCTGCTGATAAC+CGG 0.456641 5.4:+15576366 MS.gene72653:CDS
ACAATGCGTGCTTACAATAT+GGG 0.459061 5.4:+15577130 MS.gene72653:CDS
AGTAAAGTTGGTGGTGCTGA+AGG 0.482862 5.4:+15577440 MS.gene72653:CDS
TGCTAAGGCTGATAAGTTGA+AGG 0.485540 5.4:+15577093 MS.gene72653:CDS
GAATCTCACCGGTTATCAGC+AGG 0.496399 5.4:-15576374 None:intergenic
GTTCAGAGAGAGGTTTAAGA+AGG 0.508587 5.4:+15576787 MS.gene72653:CDS
GGAAAGCTGGTGGAAAGGAA+TGG 0.519414 5.4:+15576938 MS.gene72653:CDS
AGGTTGAAGCGCAAGGGCGC+TGG 0.524589 5.4:+15576483 MS.gene72653:intron
GTTTGTTAGGTTGAAGCGCA+AGG 0.526395 5.4:+15576476 MS.gene72653:intron
AAGAATGCTTCTGAGGAAGA+AGG 0.530721 5.4:+15577265 MS.gene72653:CDS
AATTAGGTTGGGAAAGCTGG+TGG 0.535794 5.4:+15576928 MS.gene72653:intron
AGGATCCTAACAAGCCTAAG+AGG 0.536126 5.4:+15576535 MS.gene72653:CDS
GGTTGGGAAAGCTGGTGGAA+AGG 0.549667 5.4:+15576933 MS.gene72653:intron
AAAACGAAGAAAGCACTTGG+AGG 0.557236 5.4:-15576558 None:intergenic
TTTGTTAGGTTGAAGCGCAA+GGG 0.561375 5.4:+15576477 MS.gene72653:intron
TAAGGCTCCATTCAATGCTA+AGG 0.574542 5.4:+15577078 MS.gene72653:CDS
AGGATGAGGAGTAAAGTTGG+TGG 0.574604 5.4:+15577431 MS.gene72653:CDS
GACTGAGAAGAATGCTTCTG+AGG 0.577758 5.4:+15577258 MS.gene72653:intron
ATGAAAACGAAGAAAGCACT+TGG 0.589018 5.4:-15576561 None:intergenic
AAGCAACAAGTCCGTTGCTG+TGG 0.608709 5.4:+15576817 MS.gene72653:CDS
TAAGCAATCGAAGAAAGCTA+AGG 0.615519 5.4:+15576515 MS.gene72653:CDS
GTTATCAGCAGGTTTAGCAT+CGG 0.618036 5.4:-15576363 None:intergenic
GGTGCTGAAGGAAGAAGCGT+AGG 0.622139 5.4:+15577452 MS.gene72653:CDS
TTAGGTCTGAGTTCAGAGAG+AGG 0.622758 5.4:+15576777 MS.gene72653:intron
CAGAAATCTCACCACAGCAA+CGG 0.627421 5.4:-15576828 None:intergenic
ATCGGAAACAGCTTTCGGCA+TGG 0.634546 5.4:-15576345 None:intergenic
TAAGGCTGATAAGTTGAAGG+AGG 0.688265 5.4:+15577096 MS.gene72653:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTATTATTCTATAATTTTTT+AGG + chr5.4:15577361-15577380 MS.gene72653:intron 10.0%
!!! TTTCATTATTTTATGTTTTC+AGG + chr5.4:15577405-15577424 MS.gene72653:intron 15.0%
!!! TTTTTGATTTTTTGTTTGTT+AGG + chr5.4:15576463-15576482 MS.gene72653:intron 15.0%
!!! ATTTTTGTTTTGCGTTTTTT+AGG + chr5.4:15576759-15576778 MS.gene72653:intron 20.0%
!!! TTGTGATTTATGTGTATTTT+AGG + chr5.4:15577054-15577073 MS.gene72653:intron 20.0%
!!! TTTATGTGTATTTTAGGATA+AGG + chr5.4:15577060-15577079 MS.gene72653:intron 20.0%
! AGAATCAAATCAAACTTCAA+CGG - chr5.4:15576735-15576754 None:intergenic 25.0%
!! CTAAATCTTAAATAGCGTTT+TGG + chr5.4:15577017-15577036 MS.gene72653:intron 25.0%
!! TCTAACGTGATTTTTGAATT+AGG + chr5.4:15576912-15576931 MS.gene72653:intron 25.0%
!! TTGCTGAATTGAACTAATTT+TGG + chr5.4:15577228-15577247 MS.gene72653:intron 25.0%
!!! CATTATTTTATGTTTTCAGG+AGG + chr5.4:15577408-15577427 MS.gene72653:intron 25.0%
CCAAAACTCAATAAACGAAA+AGG - chr5.4:15576685-15576704 None:intergenic 30.0%
! ACGTGATTTTTGAATTAGGT+TGG + chr5.4:15576916-15576935 MS.gene72653:intron 30.0%
! CCTTTTCGTTTATTGAGTTT+TGG + chr5.4:15576682-15576701 MS.gene72653:intron 30.0%
! CGTGATTTTTGAATTAGGTT+GGG + chr5.4:15576917-15576936 MS.gene72653:intron 30.0%
! CTTCGTTTTCATGTAAGATT+CGG + chr5.4:15576572-15576591 MS.gene72653:intron 30.0%
! TTCACTGTTCTGATTTTACT+CGG + chr5.4:15577194-15577213 MS.gene72653:intron 30.0%
!! TATTTTATGTTTTCAGGAGG+AGG + chr5.4:15577411-15577430 MS.gene72653:intron 30.0%
ACAATGCGTGCTTACAATAT+GGG + chr5.4:15577130-15577149 MS.gene72653:CDS 35.0%
ATCATCATTCACTTCAGACT+TGG - chr5.4:15577302-15577321 None:intergenic 35.0%
GTGCTAAAAGTTGTTCGAAT+CGG + chr5.4:15576594-15576613 MS.gene72653:intron 35.0%
TAAGCAATCGAAGAAAGCTA+AGG + chr5.4:15576515-15576534 MS.gene72653:CDS 35.0%
! TGAAGTGAATGATGATGATG+AGG + chr5.4:15577306-15577325 MS.gene72653:intron 35.0%
! TTATCAGCCTTAGCATTGAA+TGG - chr5.4:15577088-15577107 None:intergenic 35.0%
!! ATGAAAACGAAGAAAGCACT+TGG - chr5.4:15576564-15576583 None:intergenic 35.0%
AGAATCACGAATCTCGAAGT+CGG - chr5.4:15576405-15576424 None:intergenic 40.0%
ATGCTAAACCTGCTGATAAC+CGG + chr5.4:15576366-15576385 MS.gene72653:CDS 40.0%
GACAATGCGTGCTTACAATA+TGG + chr5.4:15577129-15577148 MS.gene72653:CDS 40.0%
GTTATCAGCAGGTTTAGCAT+CGG - chr5.4:15576366-15576385 None:intergenic 40.0%
GTTCAGAGAGAGGTTTAAGA+AGG + chr5.4:15576787-15576806 MS.gene72653:CDS 40.0%
TAAGGCTCCATTCAATGCTA+AGG + chr5.4:15577078-15577097 MS.gene72653:CDS 40.0%
TCGAGATTCGTGATTCTCAT+CGG + chr5.4:15576408-15576427 MS.gene72653:intron 40.0%
! AAGAATGCTTCTGAGGAAGA+AGG + chr5.4:15577265-15577284 MS.gene72653:CDS 40.0%
! GTGAATGATGATGATGAGGA+TGG + chr5.4:15577310-15577329 MS.gene72653:intron 40.0%
! TAAGGCTGATAAGTTGAAGG+AGG + chr5.4:15577096-15577115 MS.gene72653:CDS 40.0%
! TGCTAAGGCTGATAAGTTGA+AGG + chr5.4:15577093-15577112 MS.gene72653:CDS 40.0%
! TTAGCATCGGAAACAGCTTT+CGG - chr5.4:15576353-15576372 None:intergenic 40.0%
!! AAAACGAAGAAAGCACTTGG+AGG - chr5.4:15576561-15576580 None:intergenic 40.0%
!! TTGAATTAGGTTGGGAAAGC+TGG + chr5.4:15576925-15576944 MS.gene72653:intron 40.0%
!! TTTGTTAGGTTGAAGCGCAA+GGG + chr5.4:15576477-15576496 MS.gene72653:intron 40.0%
AGGAGGATGAGGAGTAAAGT+TGG + chr5.4:15577428-15577447 MS.gene72653:CDS 45.0%
AGGATCCTAACAAGCCTAAG+AGG + chr5.4:15576535-15576554 MS.gene72653:CDS 45.0%
AGGATGAGGAGTAAAGTTGG+TGG + chr5.4:15577431-15577450 MS.gene72653:CDS 45.0%
AGTCGGAAGAAGAATCTCAC+CGG - chr5.4:15576388-15576407 None:intergenic 45.0%
CAGAAATCTCACCACAGCAA+CGG - chr5.4:15576831-15576850 None:intergenic 45.0%
GACTGAGAAGAATGCTTCTG+AGG + chr5.4:15577258-15577277 MS.gene72653:intron 45.0%
TTAGGTCTGAGTTCAGAGAG+AGG + chr5.4:15576777-15576796 MS.gene72653:intron 45.0%
!! AATTAGGTTGGGAAAGCTGG+TGG + chr5.4:15576928-15576947 MS.gene72653:intron 45.0%
!! AGTAAAGTTGGTGGTGCTGA+AGG + chr5.4:15577440-15577459 MS.gene72653:CDS 45.0%
!! ATGTTTTCAGGAGGAGGATG+AGG + chr5.4:15577417-15577436 MS.gene72653:intron 45.0%
!! GTTTGTTAGGTTGAAGCGCA+AGG + chr5.4:15576476-15576495 MS.gene72653:intron 45.0%
AAGCAACAAGTCCGTTGCTG+TGG + chr5.4:15576817-15576836 MS.gene72653:CDS 50.0%
AAGCTCTGTCTGATGCTGTG+AGG + chr5.4:15576962-15576981 MS.gene72653:intron 50.0%
GGAAAGCTGGTGGAAAGGAA+TGG + chr5.4:15576938-15576957 MS.gene72653:CDS 50.0%
! AAAGCACTTGGAGGCCTCTT+AGG - chr5.4:15576552-15576571 None:intergenic 50.0%
! GAATCTCACCGGTTATCAGC+AGG - chr5.4:15576377-15576396 None:intergenic 50.0%
!! ACAGCAACGGACTTGTTGCT+TGG - chr5.4:15576818-15576837 None:intergenic 50.0%
GGTTGGGAAAGCTGGTGGAA+AGG + chr5.4:15576933-15576952 MS.gene72653:intron 55.0%
! GGAGGCCTCTTAGGCTTGTT+AGG - chr5.4:15576543-15576562 None:intergenic 55.0%
! GGTGCTGAAGGAAGAAGCGT+AGG + chr5.4:15577452-15577471 MS.gene72653:CDS 55.0%
! AGGTTGAAGCGCAAGGGCGC+TGG + chr5.4:15576483-15576502 MS.gene72653:intron 65.0%
!! AAGCGCAAGGGCGCTGGTAC+TGG + chr5.4:15576489-15576508 MS.gene72653:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 15576347 15577493 15576347 ID=MS.gene72653
chr5.4 mRNA 15576347 15577493 15576347 ID=MS.gene72653.t1;Parent=MS.gene72653
chr5.4 exon 15576347 15576387 15576347 ID=MS.gene72653.t1.exon1;Parent=MS.gene72653.t1
chr5.4 CDS 15576347 15576387 15576347 ID=cds.MS.gene72653.t1;Parent=MS.gene72653.t1
chr5.4 exon 15576485 15576583 15576485 ID=MS.gene72653.t1.exon2;Parent=MS.gene72653.t1
chr5.4 CDS 15576485 15576583 15576485 ID=cds.MS.gene72653.t1;Parent=MS.gene72653.t1
chr5.4 exon 15576781 15576838 15576781 ID=MS.gene72653.t1.exon3;Parent=MS.gene72653.t1
chr5.4 CDS 15576781 15576838 15576781 ID=cds.MS.gene72653.t1;Parent=MS.gene72653.t1
chr5.4 exon 15576934 15576978 15576934 ID=MS.gene72653.t1.exon4;Parent=MS.gene72653.t1
chr5.4 CDS 15576934 15576978 15576934 ID=cds.MS.gene72653.t1;Parent=MS.gene72653.t1
chr5.4 exon 15577076 15577156 15577076 ID=MS.gene72653.t1.exon5;Parent=MS.gene72653.t1
chr5.4 CDS 15577076 15577156 15577076 ID=cds.MS.gene72653.t1;Parent=MS.gene72653.t1
chr5.4 exon 15577259 15577302 15577259 ID=MS.gene72653.t1.exon6;Parent=MS.gene72653.t1
chr5.4 CDS 15577259 15577302 15577259 ID=cds.MS.gene72653.t1;Parent=MS.gene72653.t1
chr5.4 exon 15577427 15577493 15577427 ID=MS.gene72653.t1.exon7;Parent=MS.gene72653.t1
chr5.4 CDS 15577427 15577493 15577427 ID=cds.MS.gene72653.t1;Parent=MS.gene72653.t1
Gene Sequence

>MS.gene72653

ATGCCGAAAGCTGTTTCCGATGCTAAACCTGCTGATAACCGGTGAGATTCTTCTTCCGACTTCGAGATTCGTGATTCTCATCGGTTAATTTGTTAGATCTGAATTTTTTGTTAACGTTTTTGATTTTTTGTTTGTTAGGTTGAAGCGCAAGGGCGCTGGTACTGGAACTAAGCAATCGAAGAAAGCTAAGGATCCTAACAAGCCTAAGAGGCCTCCAAGTGCTTTCTTCGTTTTCATGTAAGATTCGGTGCTAAAAGTTGTTCGAATCGGTGCTAATTGTTCACGAATTTGTTGCAGTGAAGTTTCATTTAATTCTGACGAGTTTCGTTTTTTTTCCTTTTCGTTTATTGAGTTTTGGTGAAGTTTGAAACGTTACGAATGTGTTCCGTTGAAGTTTGATTTGATTCTAATGATTTTTGTTTTGCGTTTTTTAGGTCTGAGTTCAGAGAGAGGTTTAAGAAGGAGAATCCAAGCAACAAGTCCGTTGCTGTGGTGAGATTTCTGACACGATCTAGTTTTTATCGTTGAATTTGATTTTTATGAAATTTTGAATGTTATTGAGTTTTCTAACGTGATTTTTGAATTAGGTTGGGAAAGCTGGTGGAAAGGAATGGAAAGCTCTGTCTGATGCTGTGAGGATTTCGCTTCTGTTTAACTGAAGAAAAATAATCTAAATCTTAAATAGCGTTTTGGTTTTAAAATCGTTTTTGTGATTTATGTGTATTTTAGGATAAGGCTCCATTCAATGCTAAGGCTGATAAGTTGAAGGAGGAGTATGAGAAGACAATGCGTGCTTACAATATGGGAATTGTAATTACTTTTATAACTTTTCTGTTGCTTTGATTTTTTCACTGTTCTGATTTTACTCGGATTTTTTTTTGTTGCTGAATTGAACTAATTTTGGTGTATTAGACTGAGAAGAATGCTTCTGAGGAAGAAGGATCTGAGAAGTCCAAGTCTGAAGTGAATGATGATGATGAGGATGGTGATGATGTAAGATTTTTCTCATCGTTTGTATTATTCTATAATTTTTTAGGTTTTTATATATTGAAATTTGATTTCATTATTTTATGTTTTCAGGAGGAGGATGAGGAGTAAAGTTGGTGGTGCTGAAGGAAGAAGCGTAGGAGTAACTGCAATAGAATAA

Protein sequence

>MS.gene72653.t1

MPKAVSDAKPADNRLKRKGAGTGTKQSKKAKDPNKPKRPPSAFFVFMSEFRERFKKENPSNKSVAVVGKAGGKEWKALSDADKAPFNAKADKLKEEYEKTMRAYNMGITEKNASEEEGSEKSKRRMRSKVGGAEGRSVGVTAIE