Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055579.t1 | PNY02533.1 | 87.6 | 339 | 38 | 3 | 1 | 335 | 1 | 339 | 2.50E-151 | 545 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055579.t1 | Q197W8 | 43.6 | 479 | 141 | 12 | 1 | 356 | 1 | 473 | 1.5e-75 | 284.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055579.t1 | A0A2K3NHP5 | 87.6 | 339 | 38 | 3 | 1 | 335 | 1 | 339 | 1.8e-151 | 545.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051955 | MS.gene055579 | 0.807079 | 6.02E-50 | -1.69E-46 |
| MS.gene052143 | MS.gene055579 | 0.805499 | 1.30E-49 | -1.69E-46 |
| MS.gene052308 | MS.gene055579 | 0.843982 | 1.00E-58 | -1.69E-46 |
| MS.gene052367 | MS.gene055579 | 0.828144 | 1.06E-54 | -1.69E-46 |
| MS.gene053348 | MS.gene055579 | 0.802624 | 5.14E-49 | -1.69E-46 |
| MS.gene055308 | MS.gene055579 | 0.804068 | 2.58E-49 | -1.69E-46 |
| MS.gene055578 | MS.gene055579 | 0.858864 | 6.15E-63 | -1.69E-46 |
| MS.gene055579 | MS.gene055957 | 0.825988 | 3.47E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene056041 | 0.848774 | 4.95E-60 | -1.69E-46 |
| MS.gene055579 | MS.gene056042 | 0.865872 | 4.31E-65 | -1.69E-46 |
| MS.gene055579 | MS.gene05640 | 0.805829 | 1.11E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene057458 | 0.810466 | 1.14E-50 | -1.69E-46 |
| MS.gene055579 | MS.gene058128 | 0.807149 | 5.82E-50 | -1.69E-46 |
| MS.gene055579 | MS.gene058853 | 0.828764 | 7.50E-55 | -1.69E-46 |
| MS.gene055579 | MS.gene060473 | 0.833368 | 5.55E-56 | -1.69E-46 |
| MS.gene055579 | MS.gene060565 | 0.826161 | 3.16E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene061099 | 0.84085 | 6.78E-58 | -1.69E-46 |
| MS.gene055579 | MS.gene061260 | 0.825731 | 3.99E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene061275 | 0.832029 | 1.19E-55 | -1.69E-46 |
| MS.gene055579 | MS.gene061357 | 0.808119 | 3.62E-50 | -1.69E-46 |
| MS.gene055579 | MS.gene061366 | 0.80023 | 1.59E-48 | -1.69E-46 |
| MS.gene055579 | MS.gene061706 | 0.810476 | 1.13E-50 | -1.69E-46 |
| MS.gene055579 | MS.gene063035 | 0.844106 | 9.28E-59 | -1.69E-46 |
| MS.gene055579 | MS.gene063037 | 0.821979 | 3.03E-53 | -1.69E-46 |
| MS.gene055579 | MS.gene063095 | 0.819962 | 8.82E-53 | -1.69E-46 |
| MS.gene055579 | MS.gene064834 | 0.801374 | 9.28E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene066026 | 0.833465 | 5.25E-56 | -1.69E-46 |
| MS.gene055579 | MS.gene067143 | 0.822667 | 2.10E-53 | -1.69E-46 |
| MS.gene055579 | MS.gene067144 | 0.8327 | 8.14E-56 | -1.69E-46 |
| MS.gene055579 | MS.gene070273 | 0.827013 | 1.98E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene07051 | 0.849527 | 3.05E-60 | -1.69E-46 |
| MS.gene055579 | MS.gene072702 | 0.846942 | 1.58E-59 | -1.69E-46 |
| MS.gene055579 | MS.gene072952 | 0.834684 | 2.60E-56 | -1.69E-46 |
| MS.gene055579 | MS.gene072954 | 0.830743 | 2.47E-55 | -1.69E-46 |
| MS.gene055579 | MS.gene072956 | 0.830501 | 2.83E-55 | -1.69E-46 |
| MS.gene055579 | MS.gene072963 | 0.845628 | 3.61E-59 | -1.69E-46 |
| MS.gene055579 | MS.gene073303 | 0.812859 | 3.43E-51 | -1.69E-46 |
| MS.gene055579 | MS.gene073698 | 0.819958 | 8.83E-53 | -1.69E-46 |
| MS.gene055579 | MS.gene07616 | 0.801929 | 7.14E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene08836 | 0.83569 | 1.45E-56 | -1.69E-46 |
| MS.gene055579 | MS.gene08988 | 0.852434 | 4.63E-61 | -1.69E-46 |
| MS.gene055579 | MS.gene09002 | 0.81138 | 7.21E-51 | -1.69E-46 |
| MS.gene055579 | MS.gene09245 | 0.813092 | 3.05E-51 | -1.69E-46 |
| MS.gene055579 | MS.gene09258 | 0.846426 | 2.19E-59 | -1.69E-46 |
| MS.gene055579 | MS.gene09540 | 0.849112 | 3.99E-60 | -1.69E-46 |
| MS.gene055579 | MS.gene09543 | 0.84806 | 7.79E-60 | -1.69E-46 |
| MS.gene055579 | MS.gene09594 | 0.806167 | 9.38E-50 | -1.69E-46 |
| MS.gene055579 | MS.gene20224 | 0.810461 | 1.14E-50 | -1.69E-46 |
| MS.gene055579 | MS.gene21320 | 0.82269 | 2.07E-53 | -1.69E-46 |
| MS.gene055579 | MS.gene21734 | 0.813491 | 2.49E-51 | -1.69E-46 |
| MS.gene055579 | MS.gene21888 | 0.819171 | 1.34E-52 | -1.69E-46 |
| MS.gene055579 | MS.gene22383 | 0.816232 | 6.15E-52 | -1.69E-46 |
| MS.gene055579 | MS.gene22466 | 0.812459 | 4.20E-51 | -1.69E-46 |
| MS.gene055579 | MS.gene22500 | 0.855752 | 5.11E-62 | -1.69E-46 |
| MS.gene055579 | MS.gene23886 | 0.804633 | 1.97E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene24735 | 0.80296 | 4.38E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene25731 | 0.811726 | 6.06E-51 | -1.69E-46 |
| MS.gene055579 | MS.gene25797 | 0.833047 | 6.67E-56 | -1.69E-46 |
| MS.gene055579 | MS.gene26109 | 0.80132 | 9.52E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene27811 | 0.836939 | 6.97E-57 | -1.69E-46 |
| MS.gene055579 | MS.gene27812 | 0.837246 | 5.82E-57 | -1.69E-46 |
| MS.gene055579 | MS.gene28807 | 0.847618 | 1.03E-59 | -1.69E-46 |
| MS.gene055579 | MS.gene28867 | 0.857884 | 1.20E-62 | -1.69E-46 |
| MS.gene055579 | MS.gene28923 | 0.853426 | 2.41E-61 | -1.69E-46 |
| MS.gene055579 | MS.gene29568 | 0.837134 | 6.22E-57 | -1.69E-46 |
| MS.gene055579 | MS.gene31651 | 0.82352 | 1.33E-53 | -1.69E-46 |
| MS.gene055579 | MS.gene31653 | 0.812525 | 4.06E-51 | -1.69E-46 |
| MS.gene055579 | MS.gene31917 | 0.802411 | 5.68E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene31973 | 0.807532 | 4.83E-50 | -1.69E-46 |
| MS.gene055579 | MS.gene32467 | 0.833255 | 5.93E-56 | -1.69E-46 |
| MS.gene055579 | MS.gene32468 | 0.809079 | 2.26E-50 | -1.69E-46 |
| MS.gene055579 | MS.gene35150 | 0.817412 | 3.34E-52 | -1.69E-46 |
| MS.gene055579 | MS.gene36485 | 0.814157 | 1.78E-51 | -1.69E-46 |
| MS.gene055579 | MS.gene37573 | 0.816933 | 4.28E-52 | -1.69E-46 |
| MS.gene055579 | MS.gene37925 | 0.842843 | 2.02E-58 | -1.69E-46 |
| MS.gene055579 | MS.gene39469 | 0.815593 | 8.54E-52 | -1.69E-46 |
| MS.gene055579 | MS.gene40143 | 0.828719 | 7.69E-55 | -1.69E-46 |
| MS.gene055579 | MS.gene40329 | 0.847208 | 1.34E-59 | -1.69E-46 |
| MS.gene055579 | MS.gene40405 | 0.826011 | 3.43E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene44100 | 0.846989 | 1.54E-59 | -1.69E-46 |
| MS.gene055579 | MS.gene44103 | 0.878257 | 3.25E-69 | -1.69E-46 |
| MS.gene055579 | MS.gene44251 | 0.833409 | 5.42E-56 | -1.69E-46 |
| MS.gene055579 | MS.gene44386 | 0.801725 | 7.86E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene45040 | 0.804874 | 1.75E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene47007 | 0.826641 | 2.43E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene48067 | 0.813291 | 2.76E-51 | -1.69E-46 |
| MS.gene055579 | MS.gene48406 | 0.800514 | 1.39E-48 | -1.69E-46 |
| MS.gene055579 | MS.gene49961 | 0.807234 | 5.58E-50 | -1.69E-46 |
| MS.gene055579 | MS.gene50484 | 0.82693 | 2.07E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene50613 | 0.826748 | 2.29E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene50736 | 0.802649 | 5.08E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene52403 | 0.827614 | 1.42E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene52607 | 0.841593 | 4.32E-58 | -1.69E-46 |
| MS.gene055579 | MS.gene52703 | 0.826455 | 2.69E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene52772 | 0.801466 | 8.88E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene52980 | 0.828745 | 7.58E-55 | -1.69E-46 |
| MS.gene055579 | MS.gene53408 | 0.835796 | 1.36E-56 | -1.69E-46 |
| MS.gene055579 | MS.gene54040 | 0.824538 | 7.65E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene55817 | 0.800349 | 1.50E-48 | -1.69E-46 |
| MS.gene055579 | MS.gene57750 | 0.803679 | 3.11E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene57751 | 0.838864 | 2.23E-57 | -1.69E-46 |
| MS.gene055579 | MS.gene57752 | 0.800299 | 1.54E-48 | -1.69E-46 |
| MS.gene055579 | MS.gene58234 | 0.836856 | 7.32E-57 | -1.69E-46 |
| MS.gene055579 | MS.gene58754 | 0.828138 | 1.06E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene58835 | 0.80396 | 2.72E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene59357 | 0.800128 | 1.67E-48 | -1.69E-46 |
| MS.gene055579 | MS.gene61112 | 0.80066 | 1.30E-48 | -1.69E-46 |
| MS.gene055579 | MS.gene61966 | 0.806127 | 9.57E-50 | -1.69E-46 |
| MS.gene055579 | MS.gene61977 | 0.812241 | 4.68E-51 | -1.69E-46 |
| MS.gene055579 | MS.gene62208 | 0.84899 | 4.31E-60 | -1.69E-46 |
| MS.gene055579 | MS.gene63320 | 0.840787 | 7.05E-58 | -1.69E-46 |
| MS.gene055579 | MS.gene63469 | 0.817592 | 3.04E-52 | -1.69E-46 |
| MS.gene055579 | MS.gene64859 | 0.805418 | 1.35E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene65301 | 0.842743 | 2.15E-58 | -1.69E-46 |
| MS.gene055579 | MS.gene66403 | 0.803623 | 3.19E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene67555 | 0.827261 | 1.73E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene68361 | 0.806921 | 6.50E-50 | -1.69E-46 |
| MS.gene055579 | MS.gene69328 | 0.802268 | 6.08E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene69722 | 0.827216 | 1.77E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene70263 | 0.803893 | 2.80E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene70403 | 0.839998 | 1.13E-57 | -1.69E-46 |
| MS.gene055579 | MS.gene71431 | 0.855587 | 5.71E-62 | -1.69E-46 |
| MS.gene055579 | MS.gene72653 | 0.802155 | 6.42E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene73897 | 0.802855 | 4.60E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene74328 | 0.81813 | 2.30E-52 | -1.69E-46 |
| MS.gene055579 | MS.gene75318 | 0.854379 | 1.28E-61 | -1.69E-46 |
| MS.gene055579 | MS.gene75320 | 0.827755 | 1.31E-54 | -1.69E-46 |
| MS.gene055579 | MS.gene75642 | 0.821812 | 3.31E-53 | -1.69E-46 |
| MS.gene055579 | MS.gene77563 | 0.806381 | 8.46E-50 | -1.69E-46 |
| MS.gene055579 | MS.gene79251 | 0.813436 | 2.56E-51 | -1.69E-46 |
| MS.gene055579 | MS.gene79921 | 0.80253 | 5.37E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene80276 | 0.813146 | 2.97E-51 | -1.69E-46 |
| MS.gene055579 | MS.gene80626 | 0.840981 | 6.27E-58 | -1.69E-46 |
| MS.gene055579 | MS.gene87152 | 0.830135 | 3.48E-55 | -1.69E-46 |
| MS.gene055579 | MS.gene89653 | 0.82969 | 4.47E-55 | -1.69E-46 |
| MS.gene055579 | MS.gene90302 | 0.818518 | 1.88E-52 | -1.69E-46 |
| MS.gene055579 | MS.gene90378 | 0.807252 | 5.53E-50 | -1.69E-46 |
| MS.gene055579 | MS.gene90380 | 0.834801 | 2.43E-56 | -1.69E-46 |
| MS.gene055579 | MS.gene90876 | 0.820334 | 7.25E-53 | -1.69E-46 |
| MS.gene055579 | MS.gene90984 | 0.823405 | 1.41E-53 | -1.69E-46 |
| MS.gene055579 | MS.gene91412 | 0.80208 | 6.65E-49 | -1.69E-46 |
| MS.gene055579 | MS.gene91781 | 0.800139 | 1.66E-48 | -1.69E-46 |
| MS.gene055579 | MS.gene93102 | 0.815533 | 8.81E-52 | -1.69E-46 |
| MS.gene055579 | MS.gene93854 | 0.815028 | 1.14E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055579.t1 | MTR_1g082940 | 75.692 | 325 | 12 | 2 | 32 | 356 | 1 | 258 | 1.47e-163 | 457 |
| MS.gene055579.t1 | MTR_7g113510 | 56.512 | 430 | 99 | 12 | 1 | 351 | 1 | 421 | 1.35e-141 | 408 |
| MS.gene055579.t1 | MTR_7g113510 | 52.537 | 335 | 74 | 10 | 94 | 351 | 10 | 336 | 5.27e-91 | 276 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene055579.t1 | AT3G05330 | 49.337 | 377 | 129 | 10 | 1 | 321 | 1 | 371 | 5.55e-100 | 303 |
Find 74 sgRNAs with CRISPR-Local
Find 115 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTCTAGTAGAAGAATTTA+AGG | 0.103612 | 1.2:-60007623 | None:intergenic |
| CTATCAAATTCTTTAACTTT+TGG | 0.200274 | 1.2:+60008100 | None:intergenic |
| CCAGGTTAGTTTCACCATTA+AGG | 0.232654 | 1.2:-60007721 | MS.gene055579:CDS |
| CCTCTATTTCTTGAAATAAT+AGG | 0.242411 | 1.2:+60007923 | None:intergenic |
| AGAAACCTTAAAAGTGATTC+TGG | 0.299979 | 1.2:+60008126 | None:intergenic |
| CCTTAAATTCTTCTACTAGA+AGG | 0.318957 | 1.2:+60007623 | None:intergenic |
| GGGAATTGAACTGTTGATAC+TGG | 0.334992 | 1.2:+60007644 | None:intergenic |
| GTTTCTCCTACAAGCATTGC+AGG | 0.339255 | 1.2:+60008364 | None:intergenic |
| AGTATTTGTCTTCGGCGAAT+AGG | 0.362928 | 1.2:-60008066 | MS.gene055579:CDS |
| GTATTTGTCTTCGGCGAATA+GGG | 0.372487 | 1.2:-60008065 | MS.gene055579:CDS |
| CATTGAGTTGACTTGAACAA+AGG | 0.398174 | 1.2:+60007992 | None:intergenic |
| GGTGGAAACAAGGTTGTGTC+AGG | 0.399457 | 1.2:-60009071 | MS.gene055579:CDS |
| GCTAAAGTTCTCTTAAGCTT+TGG | 0.399461 | 1.2:+60009285 | None:intergenic |
| CTCAAACTTGCAAGTCTTGT+GGG | 0.400052 | 1.2:+60007812 | None:intergenic |
| TTGCTTGGCCTTTGAGACAT+TGG | 0.421229 | 1.2:+60008250 | None:intergenic |
| TTAGCCCATTGCCTATTCCT+TGG | 0.421233 | 1.2:+60008037 | None:intergenic |
| GAACTTCAGAACACTGCAAC+TGG | 0.423038 | 1.2:-60009092 | MS.gene055579:CDS |
| GTTGACTTGAACAAAGGGTT+GGG | 0.423813 | 1.2:+60007998 | None:intergenic |
| AGTGTTTCTCTAATGAGAAC+AGG | 0.433239 | 1.2:+60009252 | None:intergenic |
| CTTATATTTATACAGGTGAA+TGG | 0.441165 | 1.2:-60008402 | MS.gene055579:intron |
| ACACACAACTTAAGTCTAGA+AGG | 0.442768 | 1.2:-60008210 | MS.gene055579:CDS |
| AGAGAAATTGTTTCAGCAGT+TGG | 0.447693 | 1.2:-60008307 | MS.gene055579:CDS |
| TCGTTGCCTGCAATGCTTGT+AGG | 0.448297 | 1.2:-60008370 | MS.gene055579:CDS |
| ATAGGGTATCTCCAAGGAAT+AGG | 0.448663 | 1.2:-60008048 | MS.gene055579:CDS |
| AAGTTCTCTTAAGCTTTGGT+GGG | 0.451535 | 1.2:+60009289 | None:intergenic |
| CTCCTTCGAGACTAGCTACC+AGG | 0.453794 | 1.2:-60007739 | MS.gene055579:CDS |
| GCTCAAACTTGCAAGTCTTG+TGG | 0.454422 | 1.2:+60007811 | None:intergenic |
| ATCTTGACTTTGGAGTTTGA+AGG | 0.460114 | 1.2:+60007863 | None:intergenic |
| CCTATTATTTCAAGAAATAG+AGG | 0.465686 | 1.2:-60007923 | MS.gene055579:CDS |
| CTTAAATTCTTCTACTAGAA+GGG | 0.467982 | 1.2:+60007624 | None:intergenic |
| AAAGTTCTCTTAAGCTTTGG+TGG | 0.470882 | 1.2:+60009288 | None:intergenic |
| AAGCAACAAGTATTTGTCTT+CGG | 0.479578 | 1.2:-60008074 | MS.gene055579:CDS |
| AAGAGAAACTGACCTGTGTT+TGG | 0.481868 | 1.2:+60008822 | None:intergenic |
| ATTGAGTTGACTTGAACAAA+GGG | 0.497713 | 1.2:+60007993 | None:intergenic |
| GTTATCTCAAAACTAGTCTC+AGG | 0.503294 | 1.2:-60009019 | MS.gene055579:CDS |
| AGTTGACTTGAACAAAGGGT+TGG | 0.503335 | 1.2:+60007997 | None:intergenic |
| AAAAGTGATGGAATTGTAAG+TGG | 0.506447 | 1.2:-60007680 | MS.gene055579:CDS |
| CACTGCAACTGGTGGAAACA+AGG | 0.518087 | 1.2:-60009081 | MS.gene055579:CDS |
| ATCTCCAAGGAATAGGCAAT+GGG | 0.518482 | 1.2:-60008041 | MS.gene055579:CDS |
| TGACCTGTGTTTGGCGTAGA+GGG | 0.523038 | 1.2:+60008831 | None:intergenic |
| CCTTAATGGTGAAACTAACC+TGG | 0.524129 | 1.2:+60007721 | None:intergenic |
| ACTTAAGTCTAGAAGGAAGA+AGG | 0.529704 | 1.2:-60008203 | MS.gene055579:CDS |
| ATGCTTGTAGGAGAAACTCT+TGG | 0.537823 | 1.2:-60008358 | MS.gene055579:CDS |
| AGTTCTCTTAAGCTTTGGTG+GGG | 0.545383 | 1.2:+60009290 | None:intergenic |
| ATTCTCTGAAGGGTCAAGAA+TGG | 0.551911 | 1.2:-60008885 | MS.gene055579:intron |
| CTGACCTGTGTTTGGCGTAG+AGG | 0.555004 | 1.2:+60008830 | None:intergenic |
| TTGACTTTGGAGTTTGAAGG+TGG | 0.556654 | 1.2:+60007866 | None:intergenic |
| TATCTCCAAGGAATAGGCAA+TGG | 0.561784 | 1.2:-60008042 | MS.gene055579:CDS |
| AAAACTGCACAGAAAAGTGA+TGG | 0.564152 | 1.2:-60007692 | MS.gene055579:CDS |
| GGAACGAGCTCTTTGAAGTG+AGG | 0.574082 | 1.2:+60008147 | None:intergenic |
| CTTAGCCCACGAAGTACCAT+AGG | 0.574788 | 1.2:-60009041 | MS.gene055579:CDS |
| CTTCAGAACACTGCAACTGG+TGG | 0.586535 | 1.2:-60009089 | MS.gene055579:CDS |
| GAACGAGCTCTTTGAAGTGA+GGG | 0.590066 | 1.2:+60008148 | None:intergenic |
| GGAATTGAACTGTTGATACT+GGG | 0.592270 | 1.2:+60007645 | None:intergenic |
| AGATAACCTATGGTACTTCG+TGG | 0.597724 | 1.2:+60009035 | None:intergenic |
| GTGGAAACAAGGTTGTGTCA+GGG | 0.603682 | 1.2:-60009070 | MS.gene055579:CDS |
| TTTGAAGGTGGATCAATGCA+TGG | 0.607711 | 1.2:-60009126 | MS.gene055579:intron |
| TTGACTTGAACAAAGGGTTG+GGG | 0.609764 | 1.2:+60007999 | None:intergenic |
| TGGAAACAAGGTTGTGTCAG+GGG | 0.610201 | 1.2:-60009069 | MS.gene055579:CDS |
| GATAACCTATGGTACTTCGT+GGG | 0.618590 | 1.2:+60009036 | None:intergenic |
| CATTAGAGAAACACTCAACA+AGG | 0.623747 | 1.2:-60009244 | MS.gene055579:intron |
| AGGTGGATCAATGCATGGCT+CGG | 0.628242 | 1.2:-60009121 | MS.gene055579:CDS |
| CAAACTAAACTACACAATGA+CGG | 0.631209 | 1.2:-60008175 | MS.gene055579:CDS |
| GGGTCAAGAATGGTGCCACA+AGG | 0.631810 | 1.2:-60008875 | MS.gene055579:CDS |
| TGCCACAAGGAAATCTCCTG+TGG | 0.632439 | 1.2:-60008862 | MS.gene055579:CDS |
| GTAGAGGGAAACTTTCCCAC+AGG | 0.636539 | 1.2:+60008846 | None:intergenic |
| GCCACAAGGAAATCTCCTGT+GGG | 0.643915 | 1.2:-60008861 | MS.gene055579:CDS |
| ATATTTATACAGGTGAATGG+AGG | 0.648586 | 1.2:-60008399 | MS.gene055579:intron |
| GGTGCAAGCAAGAATCACTC+AGG | 0.650537 | 1.2:-60008998 | MS.gene055579:intron |
| AACCTGGTAGCTAGTCTCGA+AGG | 0.652302 | 1.2:+60007737 | None:intergenic |
| TTTCCCTCTACGCCAAACAC+AGG | 0.653931 | 1.2:-60008834 | MS.gene055579:intron |
| CGGCGAATAGGGTATCTCCA+AGG | 0.666411 | 1.2:-60008054 | MS.gene055579:CDS |
| TCGAAGGAGAGAAAGATCGA+CGG | 0.676915 | 1.2:+60007753 | None:intergenic |
| TCCCACAGGAGATTTCCTTG+TGG | 0.705582 | 1.2:+60008860 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAAATTAAACTAAAATA+TGG | + | chr1.2:60007991-60008010 | None:intergenic | 10.0% |
| !!! | AAACATTTTACATTAATCTA+AGG | + | chr1.2:60007771-60007790 | None:intergenic | 15.0% |
| !!! | AATGTAAAATGTTTCTTTTT+GGG | - | chr1.2:60007777-60007796 | MS.gene055579:CDS | 15.0% |
| !!! | TAATGTAAAATGTTTCTTTT+TGG | - | chr1.2:60007776-60007795 | MS.gene055579:CDS | 15.0% |
| !! | ATTCACCTGTATAAATATAA+GGG | + | chr1.2:60008527-60008546 | None:intergenic | 20.0% |
| !!! | ATTTCATTTTCTCAAATTAC+TGG | - | chr1.2:60008248-60008267 | MS.gene055579:CDS | 20.0% |
| !!! | CTATCAAATTCTTTAACTTT+TGG | + | chr1.2:60008831-60008850 | None:intergenic | 20.0% |
| ! | ATAATGTTCAAGTGAAAGTT+TGG | + | chr1.2:60008156-60008175 | None:intergenic | 25.0% |
| ! | CACTTGAACATTATTTAACA+TGG | - | chr1.2:60008162-60008181 | MS.gene055579:CDS | 25.0% |
| ! | CATTCACCTGTATAAATATA+AGG | + | chr1.2:60008528-60008547 | None:intergenic | 25.0% |
| ! | CCTATTATTTCAAGAAATAG+AGG | - | chr1.2:60009005-60009024 | MS.gene055579:CDS | 25.0% |
| ! | CTTATATTTATACAGGTGAA+TGG | - | chr1.2:60008526-60008545 | MS.gene055579:intron | 25.0% |
| ! | GATTTAATCAAAAACTTGTG+TGG | + | chr1.2:60009041-60009060 | None:intergenic | 25.0% |
| ! | GTTTGGAAGTAAATTTACTT+CGG | + | chr1.2:60008139-60008158 | None:intergenic | 25.0% |
| ! | TAAGTCAGACACAATTTATA+AGG | + | chr1.2:60008391-60008410 | None:intergenic | 25.0% |
| ! | TGAAATAATTAAGTCGAATG+CGG | + | chr1.2:60008234-60008253 | None:intergenic | 25.0% |
| !! | CCTCTATTTCTTGAAATAAT+AGG | + | chr1.2:60009008-60009027 | None:intergenic | 25.0% |
| !!! | CTGGTTTTAAATTGTGATTT+CGG | - | chr1.2:60008311-60008330 | MS.gene055579:CDS | 25.0% |
| !!! | TCAAATTCTTTAACTTTTGG+AGG | + | chr1.2:60008828-60008847 | None:intergenic | 25.0% |
| AAGCAACAAGTATTTGTCTT+CGG | - | chr1.2:60008854-60008873 | MS.gene055579:CDS | 30.0% | |
| AGAAACCTTAAAAGTGATTC+TGG | + | chr1.2:60008805-60008824 | None:intergenic | 30.0% | |
| ATATTTATACAGGTGAATGG+AGG | - | chr1.2:60008529-60008548 | MS.gene055579:intron | 30.0% | |
| CAAACTAAACTACACAATGA+CGG | - | chr1.2:60008753-60008772 | MS.gene055579:intron | 30.0% | |
| GTATAAATATAAGGGTTCAC+AGG | + | chr1.2:60008519-60008538 | None:intergenic | 30.0% | |
| GTGAACCCTTATATTTATAC+AGG | - | chr1.2:60008519-60008538 | MS.gene055579:intron | 30.0% | |
| ! | AAAAGTGATGGAATTGTAAG+TGG | - | chr1.2:60009248-60009267 | MS.gene055579:CDS | 30.0% |
| ! | AACTCTTGGAGAAATTTTAC+AGG | - | chr1.2:60008584-60008603 | MS.gene055579:intron | 30.0% |
| ! | TGCGAGTGTTATTACTTATT+AGG | - | chr1.2:60008457-60008476 | MS.gene055579:intron | 30.0% |
| ! | TTTTGTTACCATTCTCTGAA+GGG | - | chr1.2:60008033-60008052 | MS.gene055579:CDS | 30.0% |
| !! | ATTGAGTTGACTTGAACAAA+GGG | + | chr1.2:60008938-60008957 | None:intergenic | 30.0% |
| !! | TTTGAGACATTGGTGTTTTT+GGG | + | chr1.2:60008671-60008690 | None:intergenic | 30.0% |
| !!! | ACTAGTTTTGAGATAACCTA+TGG | + | chr1.2:60007906-60007925 | None:intergenic | 30.0% |
| !!! | GGTACTTTTTCTTTTCTTCA+TGG | - | chr1.2:60007951-60007970 | MS.gene055579:CDS | 30.0% |
| AAAACTGCACAGAAAAGTGA+TGG | - | chr1.2:60009236-60009255 | MS.gene055579:intron | 35.0% | |
| ACACACAACTTAAGTCTAGA+AGG | - | chr1.2:60008718-60008737 | MS.gene055579:intron | 35.0% | |
| ACTTAAGTCTAGAAGGAAGA+AGG | - | chr1.2:60008725-60008744 | MS.gene055579:intron | 35.0% | |
| AGAGAAATTGTTTCAGCAGT+TGG | - | chr1.2:60008621-60008640 | MS.gene055579:intron | 35.0% | |
| AGTGTTTCTCTAATGAGAAC+AGG | + | chr1.2:60007679-60007698 | None:intergenic | 35.0% | |
| CAAAAACACCAATGTCTCAA+AGG | - | chr1.2:60008670-60008689 | MS.gene055579:intron | 35.0% | |
| CATTAGAGAAACACTCAACA+AGG | - | chr1.2:60007684-60007703 | MS.gene055579:CDS | 35.0% | |
| CTTCTAATCGAAGATGTGTA+AGG | - | chr1.2:60008188-60008207 | MS.gene055579:CDS | 35.0% | |
| GCTAAAGTTCTCTTAAGCTT+TGG | + | chr1.2:60007646-60007665 | None:intergenic | 35.0% | |
| GGAATTGAACTGTTGATACT+GGG | + | chr1.2:60009286-60009305 | None:intergenic | 35.0% | |
| ! | AAAGTTCTCTTAAGCTTTGG+TGG | + | chr1.2:60007643-60007662 | None:intergenic | 35.0% |
| ! | AACAGCAGTTTTGCAAAACA+AGG | - | chr1.2:60008979-60008998 | MS.gene055579:intron | 35.0% |
| ! | AAGTTCTCTTAAGCTTTGGT+GGG | + | chr1.2:60007642-60007661 | None:intergenic | 35.0% |
| ! | AGTGCTTCATAGTCTTAATC+TGG | - | chr1.2:60008292-60008311 | MS.gene055579:CDS | 35.0% |
| ! | AGTGCTTTTGATCTTGACTT+TGG | + | chr1.2:60009078-60009097 | None:intergenic | 35.0% |
| ! | GTTATCTCAAAACTAGTCTC+AGG | - | chr1.2:60007909-60007928 | MS.gene055579:CDS | 35.0% |
| !! | AAAAACTTGTGTGGTGTTGT+TGG | + | chr1.2:60009032-60009051 | None:intergenic | 35.0% |
| !! | ACTTAAGTTGTGTGTTTTCG+AGG | + | chr1.2:60008713-60008732 | None:intergenic | 35.0% |
| !! | ATCTTGACTTTGGAGTTTGA+AGG | + | chr1.2:60009068-60009087 | None:intergenic | 35.0% |
| !! | CATTGAGTTGACTTGAACAA+AGG | + | chr1.2:60008939-60008958 | None:intergenic | 35.0% |
| !! | GTTTTGTTACCATTCTCTGA+AGG | - | chr1.2:60008032-60008051 | MS.gene055579:CDS | 35.0% |
| !! | TCGAGGTTTATTTTCTTGCT+TGG | + | chr1.2:60008696-60008715 | None:intergenic | 35.0% |
| !! | TCGTTCCAGAATCACTTTTA+AGG | - | chr1.2:60008797-60008816 | MS.gene055579:intron | 35.0% |
| !!! | CTTTGAGACATTGGTGTTTT+TGG | + | chr1.2:60008672-60008691 | None:intergenic | 35.0% |
| !!! | GTTTCTTTTTGGGTCTTTGA+AGG | - | chr1.2:60007787-60007806 | MS.gene055579:CDS | 35.0% |
| AAGAGAAACTGACCTGTGTT+TGG | + | chr1.2:60008109-60008128 | None:intergenic | 40.0% | |
| AGATAACCTATGGTACTTCG+TGG | + | chr1.2:60007896-60007915 | None:intergenic | 40.0% | |
| AGTATTTGTCTTCGGCGAAT+AGG | - | chr1.2:60008862-60008881 | MS.gene055579:CDS | 40.0% | |
| ATAAGGGTTCACAGGTGAAT+AGG | + | chr1.2:60008511-60008530 | None:intergenic | 40.0% | |
| ATCTCCAAGGAATAGGCAAT+GGG | - | chr1.2:60008887-60008906 | MS.gene055579:intron | 40.0% | |
| ATGCTTGTAGGAGAAACTCT+TGG | - | chr1.2:60008570-60008589 | MS.gene055579:intron | 40.0% | |
| ATTCTTGACCCTTCAGAGAA+TGG | + | chr1.2:60008044-60008063 | None:intergenic | 40.0% | |
| CCTTAATGGTGAAACTAACC+TGG | + | chr1.2:60009210-60009229 | None:intergenic | 40.0% | |
| GATAACCTATGGTACTTCGT+GGG | + | chr1.2:60007895-60007914 | None:intergenic | 40.0% | |
| GGGAATTGAACTGTTGATAC+TGG | + | chr1.2:60009287-60009306 | None:intergenic | 40.0% | |
| GTATTTGTCTTCGGCGAATA+GGG | - | chr1.2:60008863-60008882 | MS.gene055579:CDS | 40.0% | |
| TATCTCCAAGGAATAGGCAA+TGG | - | chr1.2:60008886-60008905 | MS.gene055579:intron | 40.0% | |
| TTCTCCAAAAAGATCGACTG+CGG | - | chr1.2:60009145-60009164 | MS.gene055579:intron | 40.0% | |
| TTTGAAGGTGGATCAATGCA+TGG | - | chr1.2:60007802-60007821 | MS.gene055579:CDS | 40.0% | |
| ! | AGTTCTCTTAAGCTTTGGTG+GGG | + | chr1.2:60007641-60007660 | None:intergenic | 40.0% |
| ! | ATAGGGTATCTCCAAGGAAT+AGG | - | chr1.2:60008880-60008899 | MS.gene055579:intron | 40.0% |
| ! | ATTCTCTGAAGGGTCAAGAA+TGG | - | chr1.2:60008043-60008062 | MS.gene055579:CDS | 40.0% |
| ! | CTCAAACTTGCAAGTCTTGT+GGG | + | chr1.2:60009119-60009138 | None:intergenic | 40.0% |
| ! | GCAGTTTTCTTGCTCCTTAA+TGG | + | chr1.2:60009224-60009243 | None:intergenic | 40.0% |
| ! | TTGACTTGAACAAAGGGTTG+GGG | + | chr1.2:60008932-60008951 | None:intergenic | 40.0% |
| !! | AGTTGACTTGAACAAAGGGT+TGG | + | chr1.2:60008934-60008953 | None:intergenic | 40.0% |
| !! | CCAGGTTAGTTTCACCATTA+AGG | - | chr1.2:60009207-60009226 | MS.gene055579:intron | 40.0% |
| !! | GTTGACTTGAACAAAGGGTT+GGG | + | chr1.2:60008933-60008952 | None:intergenic | 40.0% |
| !! | TTGACTTTGGAGTTTGAAGG+TGG | + | chr1.2:60009065-60009084 | None:intergenic | 40.0% |
| !!! | GTTTTTGGGTCATCTTTGGA+GGG | + | chr1.2:60008657-60008676 | None:intergenic | 40.0% |
| !!! | TCTTTTTGGGTCTTTGAAGG+TGG | - | chr1.2:60007790-60007809 | MS.gene055579:CDS | 40.0% |
| !!! | TGGTGTTTTTGGGTCATCTT+TGG | + | chr1.2:60008661-60008680 | None:intergenic | 40.0% |
| !!! | TGTTTTTGGGTCATCTTTGG+AGG | + | chr1.2:60008658-60008677 | None:intergenic | 40.0% |
| GAACTTCAGAACACTGCAAC+TGG | - | chr1.2:60007836-60007855 | MS.gene055579:CDS | 45.0% | |
| GCTCAAACTTGCAAGTCTTG+TGG | + | chr1.2:60009120-60009139 | None:intergenic | 45.0% | |
| GTGGAAACAAGGTTGTGTCA+GGG | - | chr1.2:60007858-60007877 | MS.gene055579:CDS | 45.0% | |
| TCGAAGGAGAGAAAGATCGA+CGG | + | chr1.2:60009178-60009197 | None:intergenic | 45.0% | |
| TGGAAACAAGGTTGTGTCAG+GGG | - | chr1.2:60007859-60007878 | MS.gene055579:CDS | 45.0% | |
| TTAGCCCATTGCCTATTCCT+TGG | + | chr1.2:60008894-60008913 | None:intergenic | 45.0% | |
| ! | GTTTCTCCTACAAGCATTGC+AGG | + | chr1.2:60008567-60008586 | None:intergenic | 45.0% |
| ! | TTAGCCGCAGTCGATCTTTT+TGG | + | chr1.2:60009152-60009171 | None:intergenic | 45.0% |
| !! | GAACGAGCTCTTTGAAGTGA+GGG | + | chr1.2:60008783-60008802 | None:intergenic | 45.0% |
| !! | TTGCTTGGCCTTTGAGACAT+TGG | + | chr1.2:60008681-60008700 | None:intergenic | 45.0% |
| AGGTGGATCAATGCATGGCT+CGG | - | chr1.2:60007807-60007826 | MS.gene055579:CDS | 50.0% | |
| CACTGCAACTGGTGGAAACA+AGG | - | chr1.2:60007847-60007866 | MS.gene055579:CDS | 50.0% | |
| CTTCAGAACACTGCAACTGG+TGG | - | chr1.2:60007839-60007858 | MS.gene055579:CDS | 50.0% | |
| GCCACAAGGAAATCTCCTGT+GGG | - | chr1.2:60008067-60008086 | MS.gene055579:CDS | 50.0% | |
| GGTGCAAGCAAGAATCACTC+AGG | - | chr1.2:60007930-60007949 | MS.gene055579:CDS | 50.0% | |
| GGTGGAAACAAGGTTGTGTC+AGG | - | chr1.2:60007857-60007876 | MS.gene055579:CDS | 50.0% | |
| GTAGAGGGAAACTTTCCCAC+AGG | + | chr1.2:60008085-60008104 | None:intergenic | 50.0% | |
| TCCCACAGGAGATTTCCTTG+TGG | + | chr1.2:60008071-60008090 | None:intergenic | 50.0% | |
| TCGTTGCCTGCAATGCTTGT+AGG | - | chr1.2:60008558-60008577 | MS.gene055579:intron | 50.0% | |
| TGACCTGTGTTTGGCGTAGA+GGG | + | chr1.2:60008100-60008119 | None:intergenic | 50.0% | |
| TGCCACAAGGAAATCTCCTG+TGG | - | chr1.2:60008066-60008085 | MS.gene055579:CDS | 50.0% | |
| TTTCCCTCTACGCCAAACAC+AGG | - | chr1.2:60008094-60008113 | MS.gene055579:CDS | 50.0% | |
| ! | AACCTGGTAGCTAGTCTCGA+AGG | + | chr1.2:60009194-60009213 | None:intergenic | 50.0% |
| ! | CTTAGCCCACGAAGTACCAT+AGG | - | chr1.2:60007887-60007906 | MS.gene055579:CDS | 50.0% |
| !! | GGAACGAGCTCTTTGAAGTG+AGG | + | chr1.2:60008784-60008803 | None:intergenic | 50.0% |
| CTGACCTGTGTTTGGCGTAG+AGG | + | chr1.2:60008101-60008120 | None:intergenic | 55.0% | |
| ! | CGGCGAATAGGGTATCTCCA+AGG | - | chr1.2:60008874-60008893 | MS.gene055579:CDS | 55.0% |
| ! | CTCCTTCGAGACTAGCTACC+AGG | - | chr1.2:60009189-60009208 | MS.gene055579:intron | 55.0% |
| !! | GGGTCAAGAATGGTGCCACA+AGG | - | chr1.2:60008053-60008072 | MS.gene055579:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 60007625 | 60009325 | 60007625 | ID=MS.gene055579 |
| chr1.2 | mRNA | 60007625 | 60009325 | 60007625 | ID=MS.gene055579.t1;Parent=MS.gene055579 |
| chr1.2 | exon | 60009245 | 60009325 | 60009245 | ID=MS.gene055579.t1.exon1;Parent=MS.gene055579.t1 |
| chr1.2 | CDS | 60009245 | 60009325 | 60009245 | ID=cds.MS.gene055579.t1;Parent=MS.gene055579.t1 |
| chr1.2 | exon | 60008999 | 60009141 | 60008999 | ID=MS.gene055579.t1.exon2;Parent=MS.gene055579.t1 |
| chr1.2 | CDS | 60008999 | 60009141 | 60008999 | ID=cds.MS.gene055579.t1;Parent=MS.gene055579.t1 |
| chr1.2 | exon | 60008835 | 60008896 | 60008835 | ID=MS.gene055579.t1.exon3;Parent=MS.gene055579.t1 |
| chr1.2 | CDS | 60008835 | 60008896 | 60008835 | ID=cds.MS.gene055579.t1;Parent=MS.gene055579.t1 |
| chr1.2 | exon | 60007625 | 60008409 | 60007625 | ID=MS.gene055579.t1.exon4;Parent=MS.gene055579.t1 |
| chr1.2 | CDS | 60007625 | 60008409 | 60007625 | ID=cds.MS.gene055579.t1;Parent=MS.gene055579.t1 |
| Gene Sequence |
| Protein sequence |
