AlfalfaGEDB | MS.gene73364

M. sativa cultivar XinJiangDaYe / MS.gene73364

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73364.t1	XP_024636636.1	100	55	0	0	1	55	1	55	1.10E-24	121.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73364.t1	P80497	80.0	25	5	0	1	25	1	25	5.5e-06	50.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73364.t1	G7JTP1	100.0	55	0	0	1	55	1	55	7.6e-25	121.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene73364	0.861588	9.23E-64	-1.69E-46
MS.gene050277	MS.gene73364	0.81669	4.86E-52	-1.69E-46
MS.gene050437	MS.gene73364	0.815774	7.78E-52	-1.69E-46
MS.gene050689	MS.gene73364	0.811593	6.48E-51	-1.69E-46
MS.gene050815	MS.gene73364	0.829695	4.46E-55	-1.69E-46
MS.gene051241	MS.gene73364	0.83519	1.94E-56	-1.69E-46
MS.gene051243	MS.gene73364	0.818217	2.20E-52	-1.69E-46
MS.gene051328	MS.gene73364	0.830894	2.27E-55	-1.69E-46
MS.gene05134	MS.gene73364	0.831589	1.53E-55	-1.69E-46
MS.gene052947	MS.gene73364	0.802638	5.10E-49	-1.69E-46
MS.gene055162	MS.gene73364	0.871718	5.52E-67	-1.69E-46
MS.gene055979	MS.gene73364	0.800384	1.48E-48	-1.69E-46
MS.gene057140	MS.gene73364	0.838184	3.34E-57	-1.69E-46
MS.gene057234	MS.gene73364	0.822973	1.78E-53	-1.69E-46
MS.gene057705	MS.gene73364	0.804722	1.88E-49	-1.69E-46
MS.gene058055	MS.gene73364	0.800867	1.18E-48	-1.69E-46
MS.gene058906	MS.gene73364	0.855025	8.32E-62	-1.69E-46
MS.gene059151	MS.gene73364	0.80959	1.75E-50	-1.69E-46
MS.gene059898	MS.gene73364	0.817782	2.76E-52	-1.69E-46
MS.gene060429	MS.gene73364	0.809879	1.52E-50	-1.69E-46
MS.gene060917	MS.gene73364	0.808572	2.90E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73364.t1	MTR_4g063080	100.000	55	0	0	1	55	1	55	4.03e-36	115
MS.gene73364.t1	MTR_2g096370	89.091	55	6	0	1	55	1	55	9.36e-32	103

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73364.t1	AT5G59613	81.818	55	10	1	55	1	55	6.89e-30	99.4
MS.gene73364.t1	AT5G59613	81.818	55	10	1	55	1	55	6.89e-30	99.4
MS.gene73364.t1	AT3G46430	81.818	55	10	1	55	1	55	6.89e-30	99.4

Find 15 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATTCTCTCTTGAAGAAAAC+TGG	0.292105	7.2:+23102113	None:intergenic
GGTAAGACCAAGAGAGAATT+TGG	0.337417	7.2:+23102037	None:intergenic
CTTGTCACCAAATTCTCTCT+TGG	0.398915	7.2:-23102044	MS.gene73364:CDS
CTCGTTGGATTCGCCGTCAC+CGG	0.441426	7.2:-23102071	MS.gene73364:CDS
CTCTTGAAGAAAACTGGCCA+TGG	0.493329	7.2:+23102119	None:intergenic
CGAATTCTTAGCATCTTCCT+CGG	0.539558	7.2:+23102016	None:intergenic
ACGGCGAATCCAACGAGGAA+TGG	0.540309	7.2:+23102077	None:intergenic
AATTCGTTCAGCATCACAAA+AGG	0.540404	7.2:-23101992	MS.gene73364:CDS
AGAGAGAATGGAAACGTACA+TGG	0.554684	7.2:-23102100	MS.gene73364:CDS
GAATTTGGTGACAAGAGCTC+CGG	0.557010	7.2:+23102052	None:intergenic
AAATGAGACAGTTCGATCCA+TGG	0.569004	7.2:-23102136	MS.gene73364:CDS
CGTACATGGCCATTCCTCGT+TGG	0.573795	7.2:-23102086	MS.gene73364:CDS
GGTGACAAGAGCTCCGGTGA+CGG	0.581739	7.2:+23102058	None:intergenic
ATTCTCTCTTGGTCTTACCG+AGG	0.686962	7.2:-23102033	MS.gene73364:CDS
CGGTGACGGCGAATCCAACG+AGG	0.732353	7.2:+23102072	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATTCGTTCAGCATCACAAA+AGG	-	chr7.2:23102131-23102150	MS.gene73364:CDS	35.0%
	CATTCTCTCTTGAAGAAAAC+TGG	+	chr7.2:23102013-23102032	None:intergenic	35.0%
!	CAGTTTTCTTCAAGAGAGAA+TGG	-	chr7.2:23102011-23102030	MS.gene73364:CDS	35.0%
	AGAGAGAATGGAAACGTACA+TGG	-	chr7.2:23102023-23102042	MS.gene73364:CDS	40.0%
	CGAATTCTTAGCATCTTCCT+CGG	+	chr7.2:23102110-23102129	None:intergenic	40.0%
	CTTGTCACCAAATTCTCTCT+TGG	-	chr7.2:23102079-23102098	MS.gene73364:CDS	40.0%
	GGTAAGACCAAGAGAGAATT+TGG	+	chr7.2:23102089-23102108	None:intergenic	40.0%
	CTCTTGAAGAAAACTGGCCA+TGG	+	chr7.2:23102007-23102026	None:intergenic	45.0%
!	GAATTTGGTGACAAGAGCTC+CGG	+	chr7.2:23102074-23102093	None:intergenic	45.0%
!!	ATTCTCTCTTGGTCTTACCG+AGG	-	chr7.2:23102090-23102109	MS.gene73364:CDS	45.0%
	ACGGCGAATCCAACGAGGAA+TGG	+	chr7.2:23102049-23102068	None:intergenic	55.0%
	CGTACATGGCCATTCCTCGT+TGG	-	chr7.2:23102037-23102056	MS.gene73364:CDS	55.0%
	CTCGTTGGATTCGCCGTCAC+CGG	-	chr7.2:23102052-23102071	MS.gene73364:CDS	60.0%
	GGTGACAAGAGCTCCGGTGA+CGG	+	chr7.2:23102068-23102087	None:intergenic	60.0%
!	CGGTGACGGCGAATCCAACG+AGG	+	chr7.2:23102054-23102073	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	23101989	23102156	23101989	ID=MS.gene73364
chr7.2	mRNA	23101989	23102156	23101989	ID=MS.gene73364.t1;Parent=MS.gene73364
chr7.2	exon	23101989	23102156	23101989	ID=MS.gene73364.t1.exon1;Parent=MS.gene73364.t1
chr7.2	CDS	23101989	23102156	23101989	ID=cds.MS.gene73364.t1;Parent=MS.gene73364.t1

Gene Sequence	>MS.gene73364 ATGAGACAGTTCGATCCATGGCCAGTTTTCTTCAAGAGAGAATGGAAACGTACATGGCCATTCCTCGTTGGATTCGCCGTCACCGGAGCTCTTGTCACCAAATTCTCTCTTGGTCTTACCGAGGAAGATGCTAAGAATTCGAAATTCGTTCAGCATCACAAAAGGTGA
Protein sequence	>MS.gene73364.t1 MRQFDPWPVFFKREWKRTWPFLVGFAVTGALVTKFSLGLTEEDAKNSKFVQHHKR