Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75387.t1 | XP_013454678.1 | 98.4 | 123 | 2 | 0 | 1 | 123 | 1 | 123 | 1.60E-68 | 268.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75387.t1 | O13801 | 32.0 | 150 | 47 | 5 | 3 | 98 | 343 | 491 | 9.1e-09 | 61.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75387.t1 | A0A072UG31 | 98.4 | 123 | 2 | 0 | 1 | 123 | 1 | 123 | 1.1e-68 | 268.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049328 | MS.gene75387 | 0.812987 | 3.21E-51 | -1.69E-46 |
| MS.gene049418 | MS.gene75387 | 0.846743 | 1.79E-59 | -1.69E-46 |
| MS.gene050310 | MS.gene75387 | 0.806286 | 8.86E-50 | -1.69E-46 |
| MS.gene050630 | MS.gene75387 | 0.804869 | 1.76E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene75387 | 0.805312 | 1.42E-49 | -1.69E-46 |
| MS.gene051248 | MS.gene75387 | 0.800652 | 1.30E-48 | -1.69E-46 |
| MS.gene051263 | MS.gene75387 | 0.82236 | 2.47E-53 | -1.69E-46 |
| MS.gene051478 | MS.gene75387 | 0.848164 | 7.30E-60 | -1.69E-46 |
| MS.gene051648 | MS.gene75387 | 0.813917 | 2.01E-51 | -1.69E-46 |
| MS.gene051720 | MS.gene75387 | 0.809126 | 2.21E-50 | -1.69E-46 |
| MS.gene053144 | MS.gene75387 | 0.817867 | 2.64E-52 | -1.69E-46 |
| MS.gene053536 | MS.gene75387 | 0.827481 | 1.53E-54 | -1.69E-46 |
| MS.gene053538 | MS.gene75387 | 0.817115 | 3.90E-52 | -1.69E-46 |
| MS.gene054584 | MS.gene75387 | 0.816491 | 5.38E-52 | -1.69E-46 |
| MS.gene055537 | MS.gene75387 | 0.823024 | 1.73E-53 | -1.69E-46 |
| MS.gene056228 | MS.gene75387 | 0.83443 | 3.01E-56 | -1.69E-46 |
| MS.gene058197 | MS.gene75387 | 0.802378 | 5.77E-49 | -1.69E-46 |
| MS.gene058198 | MS.gene75387 | 0.806421 | 8.29E-50 | -1.69E-46 |
| MS.gene05894 | MS.gene75387 | 0.836533 | 8.85E-57 | -1.69E-46 |
| MS.gene059164 | MS.gene75387 | 0.806258 | 8.98E-50 | -1.69E-46 |
| MS.gene059300 | MS.gene75387 | 0.823286 | 1.50E-53 | -1.69E-46 |
| MS.gene059424 | MS.gene75387 | 0.806181 | 9.32E-50 | -1.69E-46 |
| MS.gene060519 | MS.gene75387 | 0.838918 | 2.16E-57 | -1.69E-46 |
| MS.gene06057 | MS.gene75387 | 0.82369 | 1.21E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75387.t1 | MTR_4g011190 | 98.374 | 123 | 2 | 0 | 1 | 123 | 1 | 123 | 1.30e-84 | 243 |
| MS.gene75387.t1 | MTR_3g107610 | 58.696 | 138 | 42 | 5 | 1 | 123 | 1 | 138 | 7.67e-43 | 139 |
| MS.gene75387.t1 | MTR_3g107610 | 58.696 | 138 | 42 | 5 | 1 | 123 | 1 | 138 | 1.89e-42 | 137 |
| MS.gene75387.t1 | MTR_4g113840 | 43.802 | 121 | 53 | 3 | 5 | 123 | 6 | 113 | 1.93e-28 | 101 |
| MS.gene75387.t1 | MTR_2g054750 | 42.017 | 119 | 46 | 2 | 5 | 123 | 6 | 101 | 1.19e-25 | 94.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75387.t1 | AT3G15680 | 58.394 | 137 | 41 | 5 | 1 | 123 | 1 | 135 | 1.26e-42 | 138 |
| MS.gene75387.t1 | AT3G15680 | 58.394 | 137 | 41 | 5 | 1 | 123 | 1 | 135 | 1.26e-42 | 138 |
| MS.gene75387.t1 | AT5G25490 | 51.773 | 141 | 50 | 4 | 1 | 123 | 1 | 141 | 2.05e-34 | 117 |
| MS.gene75387.t1 | AT5G25490 | 51.773 | 141 | 50 | 4 | 1 | 123 | 1 | 141 | 2.05e-34 | 117 |
| MS.gene75387.t1 | AT2G26695 | 44.538 | 119 | 54 | 3 | 5 | 123 | 6 | 112 | 4.96e-29 | 102 |
| MS.gene75387.t1 | AT2G26695 | 44.538 | 119 | 54 | 3 | 5 | 123 | 6 | 112 | 4.96e-29 | 102 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATGTTCGTTTAGGTGAT+TGG | 0.350670 | 4.2:+89202835 | MS.gene75387:CDS |
| GTTGATTATGGATCAGTGTT+TGG | 0.365192 | 4.2:+89202774 | MS.gene75387:CDS |
| TATGGAGACAGAGTTGATTA+TGG | 0.373749 | 4.2:+89202762 | MS.gene75387:CDS |
| AGATGCGGCGATTCAAAGTA+TGG | 0.375499 | 4.2:+89202744 | MS.gene75387:CDS |
| AGTGGTTCAGATGTTCGTTT+AGG | 0.376339 | 4.2:+89202828 | MS.gene75387:CDS |
| TCAGTGTTTGGAGGAATTAG+AGG | 0.379547 | 4.2:+89202786 | MS.gene75387:CDS |
| ACAAGACCTGGATGGAAATC+TGG | 0.404499 | 4.2:+89202999 | MS.gene75387:CDS |
| ATTAGAGGAGGATCTTCATT+TGG | 0.405594 | 4.2:+89202801 | MS.gene75387:CDS |
| CTGGTGATTGGATATGCAAC+AGG | 0.442185 | 4.2:+89203018 | MS.gene75387:CDS |
| GGTGATTGGTATTGTGGTGC+TGG | 0.443291 | 4.2:+89202849 | MS.gene75387:CDS |
| GGATCTTCATTTGGTTTAAG+TGG | 0.449514 | 4.2:+89202810 | MS.gene75387:CDS |
| ACATGAGTAGGCCAGGAGAT+TGG | 0.464767 | 4.2:+89202670 | None:intergenic |
| GACCTGCAGTTCCAATCTCC+TGG | 0.472667 | 4.2:-89202681 | None:intergenic |
| GGAGTAAACATGAGTAGGCC+AGG | 0.482081 | 4.2:+89202663 | None:intergenic |
| GCCTTTGAAAGTTCATGTGC+TGG | 0.482420 | 4.2:-89202707 | None:intergenic |
| CTGGATGGAAATCTGGTGAT+TGG | 0.494361 | 4.2:+89203006 | MS.gene75387:CDS |
| CAATCACCAGATTTCCATCC+AGG | 0.500432 | 4.2:-89203005 | None:intergenic |
| CGTTTAGGTGATTGGTATTG+TGG | 0.513265 | 4.2:+89202843 | MS.gene75387:CDS |
| GGCCAGGAGATTGGAACTGC+AGG | 0.518043 | 4.2:+89202679 | MS.gene75387:CDS |
| GCCAGCACATGAACTTTCAA+AGG | 0.538078 | 4.2:+89202706 | MS.gene75387:CDS |
| TATTGTGGTGCTGGTAATTG+TGG | 0.545221 | 4.2:+89202858 | MS.gene75387:CDS |
| GATTATGGATCAGTGTTTGG+AGG | 0.553466 | 4.2:+89202777 | MS.gene75387:CDS |
| AAAGATGACCTAGCTGGTGG+AGG | 0.570316 | 4.2:+89202930 | MS.gene75387:CDS |
| CGTTTAAGTTGCTTCAAATG+TGG | 0.570358 | 4.2:+89202900 | MS.gene75387:CDS |
| TATTATATCCTCCACCAGCT+AGG | 0.570596 | 4.2:-89202938 | None:intergenic |
| CCAAAGGAGTAAACATGAGT+AGG | 0.578304 | 4.2:+89202658 | None:intergenic |
| TTTGGTGGAAATACAAGACC+TGG | 0.581005 | 4.2:+89202987 | MS.gene75387:CDS |
| GCTTTCAAAGATGACCTAGC+TGG | 0.598108 | 4.2:+89202924 | MS.gene75387:CDS |
| TTCAAAGATGACCTAGCTGG+TGG | 0.604459 | 4.2:+89202927 | MS.gene75387:CDS |
| GTGGAAATACAAGACCTGGA+TGG | 0.606718 | 4.2:+89202991 | MS.gene75387:CDS |
| CGCGAATCATGTCAAAGATG+CGG | 0.623391 | 4.2:+89202729 | MS.gene75387:CDS |
| GTGTTTGGAGGAATTAGAGG+AGG | 0.633226 | 4.2:+89202789 | MS.gene75387:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTTTGCTTTCAAGAGGTTT+TGG | + | chr4.2:89202969-89202988 | MS.gene75387:CDS | 30.0% |
| ATTAGAGGAGGATCTTCATT+TGG | + | chr4.2:89202801-89202820 | MS.gene75387:CDS | 35.0% | |
| GTTGATTATGGATCAGTGTT+TGG | + | chr4.2:89202774-89202793 | MS.gene75387:CDS | 35.0% | |
| ! | AGTGACATTTTGCTTTCAAG+AGG | + | chr4.2:89202963-89202982 | MS.gene75387:CDS | 35.0% |
| ! | GGATCTTCATTTGGTTTAAG+TGG | + | chr4.2:89202810-89202829 | MS.gene75387:CDS | 35.0% |
| ! | TATGGAGACAGAGTTGATTA+TGG | + | chr4.2:89202762-89202781 | MS.gene75387:CDS | 35.0% |
| !! | CGTTTAAGTTGCTTCAAATG+TGG | + | chr4.2:89202900-89202919 | MS.gene75387:CDS | 35.0% |
| AGTGGTTCAGATGTTCGTTT+AGG | + | chr4.2:89202828-89202847 | MS.gene75387:CDS | 40.0% | |
| CAGATGTTCGTTTAGGTGAT+TGG | + | chr4.2:89202835-89202854 | MS.gene75387:CDS | 40.0% | |
| CGTTTAGGTGATTGGTATTG+TGG | + | chr4.2:89202843-89202862 | MS.gene75387:CDS | 40.0% | |
| GATTATGGATCAGTGTTTGG+AGG | + | chr4.2:89202777-89202796 | MS.gene75387:CDS | 40.0% | |
| TATTATATCCTCCACCAGCT+AGG | - | chr4.2:89202941-89202960 | None:intergenic | 40.0% | |
| TCAGTGTTTGGAGGAATTAG+AGG | + | chr4.2:89202786-89202805 | MS.gene75387:CDS | 40.0% | |
| TTTGGTGGAAATACAAGACC+TGG | + | chr4.2:89202987-89203006 | MS.gene75387:CDS | 40.0% | |
| !! | TATTGTGGTGCTGGTAATTG+TGG | + | chr4.2:89202858-89202877 | MS.gene75387:CDS | 40.0% |
| !! | TTGCTTTCAAGAGGTTTTGG+TGG | + | chr4.2:89202972-89202991 | MS.gene75387:CDS | 40.0% |
| ACAAGACCTGGATGGAAATC+TGG | + | chr4.2:89202999-89203018 | MS.gene75387:CDS | 45.0% | |
| AGATGCGGCGATTCAAAGTA+TGG | + | chr4.2:89202744-89202763 | MS.gene75387:CDS | 45.0% | |
| CAATCACCAGATTTCCATCC+AGG | - | chr4.2:89203008-89203027 | None:intergenic | 45.0% | |
| CGCGAATCATGTCAAAGATG+CGG | + | chr4.2:89202729-89202748 | MS.gene75387:CDS | 45.0% | |
| CTGGATGGAAATCTGGTGAT+TGG | + | chr4.2:89203006-89203025 | MS.gene75387:CDS | 45.0% | |
| CTGGTGATTGGATATGCAAC+AGG | + | chr4.2:89203018-89203037 | MS.gene75387:CDS | 45.0% | |
| GCCAGCACATGAACTTTCAA+AGG | + | chr4.2:89202706-89202725 | MS.gene75387:CDS | 45.0% | |
| GCTTTCAAAGATGACCTAGC+TGG | + | chr4.2:89202924-89202943 | MS.gene75387:CDS | 45.0% | |
| GTGGAAATACAAGACCTGGA+TGG | + | chr4.2:89202991-89203010 | MS.gene75387:CDS | 45.0% | |
| GTGTTTGGAGGAATTAGAGG+AGG | + | chr4.2:89202789-89202808 | MS.gene75387:CDS | 45.0% | |
| TTCAAAGATGACCTAGCTGG+TGG | + | chr4.2:89202927-89202946 | MS.gene75387:CDS | 45.0% | |
| ! | GCCTTTGAAAGTTCATGTGC+TGG | - | chr4.2:89202710-89202729 | None:intergenic | 45.0% |
| AAAGATGACCTAGCTGGTGG+AGG | + | chr4.2:89202930-89202949 | MS.gene75387:CDS | 50.0% | |
| GGTGATTGGTATTGTGGTGC+TGG | + | chr4.2:89202849-89202868 | MS.gene75387:CDS | 50.0% | |
| GACCTGCAGTTCCAATCTCC+TGG | - | chr4.2:89202684-89202703 | None:intergenic | 55.0% | |
| GGCCAGGAGATTGGAACTGC+AGG | + | chr4.2:89202679-89202698 | MS.gene75387:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 89202672 | 89203043 | 89202672 | ID=MS.gene75387 |
| chr4.2 | mRNA | 89202672 | 89203043 | 89202672 | ID=MS.gene75387.t1;Parent=MS.gene75387 |
| chr4.2 | exon | 89202672 | 89203043 | 89202672 | ID=MS.gene75387.t1.exon1;Parent=MS.gene75387.t1 |
| chr4.2 | CDS | 89202672 | 89203043 | 89202672 | ID=cds.MS.gene75387.t1;Parent=MS.gene75387.t1 |
| Gene Sequence |
| Protein sequence |
