Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77228.t1 | XP_003622954.1 | 95.6 | 476 | 21 | 0 | 5 | 480 | 40 | 515 | 6.20E-254 | 886.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77228.t1 | G7L1B6 | 95.6 | 476 | 21 | 0 | 5 | 480 | 40 | 515 | 4.5e-254 | 886.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049351 | MS.gene77228 | 0.803019 | 4.26E-49 | -1.69E-46 |
| MS.gene049491 | MS.gene77228 | 0.813943 | 1.98E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene77228 | 0.818295 | 2.11E-52 | -1.69E-46 |
| MS.gene050076 | MS.gene77228 | 0.82538 | 4.84E-54 | -1.69E-46 |
| MS.gene050301 | MS.gene77228 | 0.831678 | 1.46E-55 | -1.69E-46 |
| MS.gene050397 | MS.gene77228 | 0.803779 | 2.96E-49 | -1.69E-46 |
| MS.gene050432 | MS.gene77228 | 0.821634 | 3.64E-53 | -1.69E-46 |
| MS.gene05101 | MS.gene77228 | 0.85217 | 5.50E-61 | -1.69E-46 |
| MS.gene051471 | MS.gene77228 | 0.809786 | 1.59E-50 | -1.69E-46 |
| MS.gene052287 | MS.gene77228 | 0.806096 | 9.71E-50 | -1.69E-46 |
| MS.gene052310 | MS.gene77228 | 0.815291 | 9.97E-52 | -1.69E-46 |
| MS.gene052326 | MS.gene77228 | 0.837439 | 5.20E-57 | -1.69E-46 |
| MS.gene053836 | MS.gene77228 | 0.820275 | 7.47E-53 | -1.69E-46 |
| MS.gene054376 | MS.gene77228 | 0.800721 | 1.26E-48 | -1.69E-46 |
| MS.gene05455 | MS.gene77228 | 0.823764 | 1.16E-53 | -1.69E-46 |
| MS.gene054803 | MS.gene77228 | 0.802237 | 6.17E-49 | -1.69E-46 |
| MS.gene055277 | MS.gene77228 | 0.84915 | 3.89E-60 | -1.69E-46 |
| MS.gene055390 | MS.gene77228 | 0.838809 | 2.31E-57 | -1.69E-46 |
| MS.gene055422 | MS.gene77228 | 0.818504 | 1.89E-52 | -1.69E-46 |
| MS.gene055428 | MS.gene77228 | 0.821812 | 3.31E-53 | -1.69E-46 |
| MS.gene056191 | MS.gene77228 | 0.806935 | 6.46E-50 | -1.69E-46 |
| MS.gene057121 | MS.gene77228 | 0.802586 | 5.23E-49 | -1.69E-46 |
| MS.gene05733 | MS.gene77228 | 0.804494 | 2.10E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene77228 | 0.814659 | 1.38E-51 | -1.69E-46 |
| MS.gene05811 | MS.gene77228 | 0.82835 | 9.44E-55 | -1.69E-46 |
| MS.gene058660 | MS.gene77228 | 0.832717 | 8.06E-56 | -1.69E-46 |
| MS.gene05907 | MS.gene77228 | 0.83055 | 2.76E-55 | -1.69E-46 |
| MS.gene059326 | MS.gene77228 | 0.819248 | 1.28E-52 | -1.69E-46 |
| MS.gene059518 | MS.gene77228 | 0.801455 | 8.93E-49 | -1.69E-46 |
| MS.gene059536 | MS.gene77228 | 0.801172 | 1.02E-48 | -1.69E-46 |
| MS.gene059597 | MS.gene77228 | 0.811672 | 6.23E-51 | -1.69E-46 |
| MS.gene059688 | MS.gene77228 | 0.814072 | 1.86E-51 | -1.69E-46 |
| MS.gene059692 | MS.gene77228 | 0.82633 | 2.88E-54 | -1.69E-46 |
| MS.gene059695 | MS.gene77228 | 0.827957 | 1.17E-54 | -1.69E-46 |
| MS.gene059894 | MS.gene77228 | 0.808958 | 2.40E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77228.t1 | MTR_7g058760 | 95.588 | 476 | 21 | 0 | 5 | 480 | 40 | 515 | 0.0 | 918 |
| MS.gene77228.t1 | MTR_7g058760 | 94.958 | 476 | 21 | 1 | 5 | 480 | 40 | 512 | 0.0 | 907 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77228.t1 | AT5G48830 | 45.598 | 443 | 232 | 4 | 44 | 480 | 72 | 511 | 2.65e-129 | 385 |
| MS.gene77228.t1 | AT5G48830 | 44.889 | 450 | 231 | 6 | 44 | 480 | 72 | 517 | 1.31e-124 | 373 |
Find 87 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTCAAGCCGAGTGTATT+TGG | 0.164191 | 7.2:-37283306 | MS.gene77228:CDS |
| CGTGACAGCTCAGACATTTC+TGG | 0.224603 | 7.2:-37283057 | MS.gene77228:CDS |
| CCTATGCCAATGCAATTTAA+AGG | 0.247757 | 7.2:+37283243 | None:intergenic |
| TATAACTAAATCTTCTAAAA+TGG | 0.287288 | 7.2:+37280039 | None:intergenic |
| AGCTTGATTGGCAGGTCTTT+AGG | 0.295665 | 7.2:-37276341 | MS.gene77228:CDS |
| CACCATGGGTAGCAAATATA+CGG | 0.297562 | 7.2:+37280065 | None:intergenic |
| AATTCAAGCCGAGTGTATTT+GGG | 0.298989 | 7.2:-37283305 | MS.gene77228:CDS |
| AAGTATATTGCTCACTTATA+TGG | 0.317130 | 7.2:-37283148 | MS.gene77228:CDS |
| TGGTGTTGCAAGCTTCTATC+TGG | 0.360271 | 7.2:-37280005 | MS.gene77228:CDS |
| TCCAAAGTTAAGGTTACTTT+GGG | 0.362833 | 7.2:-37282774 | MS.gene77228:CDS |
| TTACTTTGGTATCTTACTTT+CGG | 0.365779 | 7.2:-37280825 | MS.gene77228:CDS |
| GTCCAAAGTTAAGGTTACTT+TGG | 0.374599 | 7.2:-37282775 | MS.gene77228:CDS |
| TTGCATTGGCATAGGAGCTT+TGG | 0.387344 | 7.2:-37283235 | MS.gene77228:CDS |
| TCCACTGGTTGTACAGAAAC+AGG | 0.392162 | 7.2:+37283457 | None:intergenic |
| AAGGAATTAAGAGCCCTTAC+AGG | 0.396255 | 7.2:-37277440 | MS.gene77228:CDS |
| ATCTTACTTTCGGTTTGGTC+AGG | 0.397668 | 7.2:-37280815 | MS.gene77228:intron |
| TCTTTGTTGCTTCTATTGCC+TGG | 0.403195 | 7.2:-37283433 | MS.gene77228:intron |
| TCTTTAGGACTCATTTATAC+AGG | 0.405898 | 7.2:-37276326 | MS.gene77228:CDS |
| TTGCCGAGGGTCCAAAGTTA+AGG | 0.405993 | 7.2:-37282784 | MS.gene77228:CDS |
| TTGGTATCTTACTTTCGGTT+TGG | 0.406710 | 7.2:-37280820 | MS.gene77228:CDS |
| TCTAAATGCATTAGAGAAAG+AGG | 0.418471 | 7.2:-37282864 | MS.gene77228:CDS |
| TTAATAACTGTTCTAACAAT+CGG | 0.419593 | 7.2:+37276398 | None:intergenic |
| TTCTATCTCTGCTACATGGC+TGG | 0.421666 | 7.2:-37282144 | MS.gene77228:CDS |
| ATCTGTATTCAGAATTACTT+TGG | 0.422723 | 7.2:-37280839 | MS.gene77228:CDS |
| CGGGTATAAGATTTGTCAAC+AGG | 0.422732 | 7.2:+37283114 | None:intergenic |
| CGTTTCGAGTTCTGTTGGTA+TGG | 0.426492 | 7.2:-37282213 | MS.gene77228:CDS |
| CTATGCCAATGCAATTTAAA+GGG | 0.428572 | 7.2:+37283244 | None:intergenic |
| TGGTGGAACAGCATCTAATT+TGG | 0.431361 | 7.2:-37283202 | MS.gene77228:CDS |
| GACTCATTTATACAGGCATT+AGG | 0.445461 | 7.2:-37276319 | MS.gene77228:CDS |
| TTGTCAGTTTGACTGCTGTC+TGG | 0.445807 | 7.2:+37277566 | None:intergenic |
| CTCTGCTACATGGCTGGAAA+AGG | 0.447443 | 7.2:-37282138 | MS.gene77228:CDS |
| TTGCAGTGCGGTGCAGCAAA+AGG | 0.455706 | 7.2:-37282942 | MS.gene77228:intron |
| ACTGCTGTCTGGTAAATCAA+TGG | 0.457783 | 7.2:+37277577 | None:intergenic |
| GCCCAAAGTAACCTTAACTT+TGG | 0.458602 | 7.2:+37282773 | None:intergenic |
| AAAAGAAACTGATTAACAAC+TGG | 0.461741 | 7.2:+37283177 | None:intergenic |
| AGAGAACATACCATCCTGTA+AGG | 0.466818 | 7.2:+37277426 | None:intergenic |
| AGAAGATTTAGTTATAGCTC+TGG | 0.472303 | 7.2:-37280032 | MS.gene77228:CDS |
| GTCAAGTAAAATAAATAGCT+TGG | 0.479677 | 7.2:-37279957 | MS.gene77228:CDS |
| ACTAAGCTCACTTCCATTTG+AGG | 0.483628 | 7.2:+37276291 | None:intergenic |
| GCATTCCCTTTAAATTGCAT+TGG | 0.484750 | 7.2:-37283249 | MS.gene77228:CDS |
| CTAAGCTCACTTCCATTTGA+GGG | 0.487932 | 7.2:+37276292 | None:intergenic |
| GCATTAGGCAGTCCCTCAAA+TGG | 0.493967 | 7.2:-37276304 | MS.gene77228:CDS |
| GTACAGAACATTACAAGGTC+TGG | 0.502247 | 7.2:-37280870 | MS.gene77228:CDS |
| ATATTGCTCACTTATATGGC+TGG | 0.502407 | 7.2:-37283144 | MS.gene77228:CDS |
| TCCCGTATATTTGCTACCCA+TGG | 0.505444 | 7.2:-37280067 | MS.gene77228:CDS |
| GGAGCTTTGGTTGACTCTGA+TGG | 0.508159 | 7.2:-37283222 | MS.gene77228:CDS |
| CCTTTAAATTGCATTGGCAT+AGG | 0.508640 | 7.2:-37283243 | MS.gene77228:CDS |
| TTCAGGTGGCTTTGAACCAG+TGG | 0.510210 | 7.2:-37277673 | MS.gene77228:intron |
| CTGCTGTCTGGTAAATCAAT+GGG | 0.514204 | 7.2:+37277578 | None:intergenic |
| ATTTCTCAATGCCCATAAAA+CGG | 0.516499 | 7.2:-37277466 | MS.gene77228:CDS |
| TAACCTTAACTTTGGACCCT+CGG | 0.519078 | 7.2:+37282781 | None:intergenic |
| TTAGAGAAAGAGGCTTATGC+GGG | 0.522119 | 7.2:-37282854 | MS.gene77228:CDS |
| TCCTGTTTCTGTACAACCAG+TGG | 0.523707 | 7.2:-37283458 | MS.gene77228:CDS |
| GTCATTCTATCTCTGCTACA+TGG | 0.534757 | 7.2:-37282148 | MS.gene77228:CDS |
| TCAAGCGTTTCGAGTTCTGT+TGG | 0.539894 | 7.2:-37282218 | MS.gene77228:CDS |
| CACAGTTGTGTCAATGTATG+AGG | 0.540761 | 7.2:-37277633 | MS.gene77228:CDS |
| CCATGTTGTGGTACAACCAC+TGG | 0.543771 | 7.2:+37277657 | None:intergenic |
| AGTTTAAAAGCTGTTGCCGA+GGG | 0.560847 | 7.2:-37282797 | MS.gene77228:CDS |
| TTCTAAAATGGAAACACCAT+GGG | 0.562854 | 7.2:+37280051 | None:intergenic |
| TGTCAGACAGCTCAAGAAGA+AGG | 0.563566 | 7.2:+37276429 | None:intergenic |
| TTAGTTATAGCTCTGGCAGA+TGG | 0.564419 | 7.2:-37280025 | MS.gene77228:CDS |
| GTCAAACTGACAAACAAAGT+TGG | 0.568058 | 7.2:-37277556 | MS.gene77228:CDS |
| AACTGTTCTAACAATCGGCA+TGG | 0.578091 | 7.2:+37276403 | None:intergenic |
| TGTAAAAGGCAACACTCACC+AGG | 0.584076 | 7.2:-37282105 | MS.gene77228:intron |
| ACCATGGGTAGCAAATATAC+GGG | 0.584532 | 7.2:+37280066 | None:intergenic |
| CTTCTAAAATGGAAACACCA+TGG | 0.584695 | 7.2:+37280050 | None:intergenic |
| ATTCAAGCCGAGTGTATTTG+GGG | 0.587572 | 7.2:-37283304 | MS.gene77228:CDS |
| AAGTAAAATAAATAGCTTGG+AGG | 0.590770 | 7.2:-37279954 | MS.gene77228:CDS |
| AAACTGACAAACAAAGTTGG+TGG | 0.594439 | 7.2:-37277553 | MS.gene77228:CDS |
| TTAAAGGGAATGCCCGAAGA+CGG | 0.596553 | 7.2:+37283259 | None:intergenic |
| AGAAGCAACAAAGATTCCAC+TGG | 0.596892 | 7.2:+37283442 | None:intergenic |
| CCAGTGGTTGTACCACAACA+TGG | 0.599413 | 7.2:-37277657 | MS.gene77228:CDS |
| ATTAGAGAAAGAGGCTTATG+CGG | 0.602745 | 7.2:-37282855 | MS.gene77228:CDS |
| TTATTGTACAGAACATTACA+AGG | 0.605422 | 7.2:-37280875 | MS.gene77228:CDS |
| GAGTTTAAAAGCTGTTGCCG+AGG | 0.616763 | 7.2:-37282798 | MS.gene77228:CDS |
| GCTTTGGTTGACTCTGATGG+TGG | 0.626948 | 7.2:-37283219 | MS.gene77228:CDS |
| TGTCTGGTAAATCAATGGGT+TGG | 0.629314 | 7.2:+37277582 | None:intergenic |
| AATTAAGAGCCCTTACAGGA+TGG | 0.631964 | 7.2:-37277436 | MS.gene77228:intron |
| AATGCCCATAAAACGGACAA+AGG | 0.635965 | 7.2:-37277459 | MS.gene77228:CDS |
| GAGAACATACCATCCTGTAA+GGG | 0.636010 | 7.2:+37277427 | None:intergenic |
| ACACAACTGTGTCCATGTTG+TGG | 0.650889 | 7.2:+37277645 | None:intergenic |
| TCTGCAGCCCCAAATACACT+CGG | 0.654793 | 7.2:+37283297 | None:intergenic |
| GTCAAATTATGTATTAGATG+TGG | 0.662552 | 7.2:-37282909 | MS.gene77228:CDS |
| ACAATATAACTCATGTGACA+TGG | 0.676870 | 7.2:+37277502 | None:intergenic |
| ACAGCTCAGACATTTCTGGT+AGG | 0.677195 | 7.2:-37283053 | MS.gene77228:intron |
| TAGAGAAAGAGGCTTATGCG+GGG | 0.683596 | 7.2:-37282853 | MS.gene77228:CDS |
| AGAGAAAGAGGCTTATGCGG+GGG | 0.688620 | 7.2:-37282852 | MS.gene77228:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 37276298 | 37283487 | 37276298 | ID=MS.gene77228 |
| chr7.2 | mRNA | 37276298 | 37283487 | 37276298 | ID=MS.gene77228.t1;Parent=MS.gene77228 |
| chr7.2 | exon | 37283434 | 37283487 | 37283434 | ID=MS.gene77228.t1.exon1;Parent=MS.gene77228.t1 |
| chr7.2 | CDS | 37283434 | 37283487 | 37283434 | ID=cds.MS.gene77228.t1;Parent=MS.gene77228.t1 |
| chr7.2 | exon | 37283054 | 37283331 | 37283054 | ID=MS.gene77228.t1.exon2;Parent=MS.gene77228.t1 |
| chr7.2 | CDS | 37283054 | 37283331 | 37283054 | ID=cds.MS.gene77228.t1;Parent=MS.gene77228.t1 |
| chr7.2 | exon | 37282748 | 37282958 | 37282748 | ID=MS.gene77228.t1.exon3;Parent=MS.gene77228.t1 |
| chr7.2 | CDS | 37282748 | 37282958 | 37282748 | ID=cds.MS.gene77228.t1;Parent=MS.gene77228.t1 |
| chr7.2 | exon | 37282106 | 37282252 | 37282106 | ID=MS.gene77228.t1.exon4;Parent=MS.gene77228.t1 |
| chr7.2 | CDS | 37282106 | 37282252 | 37282106 | ID=cds.MS.gene77228.t1;Parent=MS.gene77228.t1 |
| chr7.2 | exon | 37280816 | 37280940 | 37280816 | ID=MS.gene77228.t1.exon5;Parent=MS.gene77228.t1 |
| chr7.2 | CDS | 37280816 | 37280940 | 37280816 | ID=cds.MS.gene77228.t1;Parent=MS.gene77228.t1 |
| chr7.2 | exon | 37279904 | 37280108 | 37279904 | ID=MS.gene77228.t1.exon6;Parent=MS.gene77228.t1 |
| chr7.2 | CDS | 37279904 | 37280108 | 37279904 | ID=cds.MS.gene77228.t1;Parent=MS.gene77228.t1 |
| chr7.2 | exon | 37277437 | 37277690 | 37277437 | ID=MS.gene77228.t1.exon7;Parent=MS.gene77228.t1 |
| chr7.2 | CDS | 37277437 | 37277690 | 37277437 | ID=cds.MS.gene77228.t1;Parent=MS.gene77228.t1 |
| chr7.2 | exon | 37276298 | 37276466 | 37276298 | ID=MS.gene77228.t1.exon8;Parent=MS.gene77228.t1 |
| chr7.2 | CDS | 37276298 | 37276466 | 37276298 | ID=cds.MS.gene77228.t1;Parent=MS.gene77228.t1 |
| Gene Sequence |
| Protein sequence |
