Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79112.t1 | AES72057.2 | 98.3 | 175 | 3 | 0 | 1 | 175 | 206 | 380 | 1.90E-83 | 318.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79112.t1 | Q8GYF7 | 70.9 | 165 | 48 | 0 | 1 | 165 | 206 | 370 | 1.1e-55 | 217.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79112.t1 | G7JC14 | 98.3 | 175 | 3 | 0 | 1 | 175 | 206 | 380 | 1.4e-83 | 318.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049824 | MS.gene79112 | 0.813893 | 2.03E-51 | -1.69E-46 |
| MS.gene049825 | MS.gene79112 | 0.8116 | 6.46E-51 | -1.69E-46 |
| MS.gene049850 | MS.gene79112 | 0.807302 | 5.40E-50 | -1.69E-46 |
| MS.gene049873 | MS.gene79112 | 0.815639 | 8.34E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene79112 | 0.817445 | 3.29E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene79112 | 0.824622 | 7.31E-54 | -1.69E-46 |
| MS.gene052519 | MS.gene79112 | 0.813585 | 2.38E-51 | -1.69E-46 |
| MS.gene052582 | MS.gene79112 | 0.833778 | 4.39E-56 | -1.69E-46 |
| MS.gene052584 | MS.gene79112 | 0.830104 | 3.54E-55 | -1.69E-46 |
| MS.gene052879 | MS.gene79112 | 0.801388 | 9.22E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene79112 | 0.841312 | 5.13E-58 | -1.69E-46 |
| MS.gene053599 | MS.gene79112 | 0.807461 | 5.00E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene79112 | 0.819658 | 1.03E-52 | -1.69E-46 |
| MS.gene055166 | MS.gene79112 | 0.801833 | 7.47E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene79112 | 0.800507 | 1.40E-48 | -1.69E-46 |
| MS.gene055694 | MS.gene79112 | 0.809297 | 2.03E-50 | -1.69E-46 |
| MS.gene055778 | MS.gene79112 | 0.813954 | 1.97E-51 | -1.69E-46 |
| MS.gene055808 | MS.gene79112 | 0.8134 | 2.61E-51 | -1.69E-46 |
| MS.gene056215 | MS.gene79112 | 0.826626 | 2.45E-54 | -1.69E-46 |
| MS.gene056642 | MS.gene79112 | 0.800619 | 1.32E-48 | -1.69E-46 |
| MS.gene057314 | MS.gene79112 | 0.801525 | 8.64E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene79112 | 0.810545 | 1.09E-50 | -1.69E-46 |
| MS.gene057915 | MS.gene79112 | 0.811395 | 7.15E-51 | -1.69E-46 |
| MS.gene058037 | MS.gene79112 | 0.824101 | 9.68E-54 | -1.69E-46 |
| MS.gene058625 | MS.gene79112 | 0.82017 | 7.90E-53 | -1.69E-46 |
| MS.gene059015 | MS.gene79112 | 0.813154 | 2.95E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene79112 | 0.839632 | 1.41E-57 | -1.69E-46 |
| MS.gene059625 | MS.gene79112 | 0.813362 | 2.66E-51 | -1.69E-46 |
| MS.gene060429 | MS.gene79112 | 0.821292 | 4.36E-53 | -1.69E-46 |
| MS.gene060521 | MS.gene79112 | 0.814013 | 1.91E-51 | -1.69E-46 |
| MS.gene060587 | MS.gene79112 | 0.809272 | 2.05E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79112.t1 | MTR_3g085590 | 98.286 | 175 | 3 | 0 | 1 | 175 | 206 | 380 | 3.06e-122 | 350 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79112.t1 | AT1G24310 | 70.909 | 165 | 48 | 0 | 1 | 165 | 206 | 370 | 6.55e-78 | 237 |
Find 40 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGACTTTGATCGTGAATTT+TGG | 0.193440 | 3.2:-73495731 | None:intergenic |
| TGGTAACTCAGCTCATCTTC+TGG | 0.278398 | 3.2:+73495702 | MS.gene79112:CDS |
| TATCTGCTTGAAAATGTCTT+TGG | 0.301845 | 3.2:-73495265 | None:intergenic |
| AAGGCTAATAGCAATGAATT+TGG | 0.302986 | 3.2:+73495682 | MS.gene79112:CDS |
| GAAGCGAGACGCACGGGATA+TGG | 0.316915 | 3.2:+73495972 | MS.gene79112:CDS |
| AACCCAAAGCAATGTTAAAA+TGG | 0.323703 | 3.2:+73495146 | MS.gene79112:CDS |
| GACATGGGAACCTCAGAAAA+TGG | 0.331518 | 3.2:+73496012 | MS.gene79112:CDS |
| GGTAACTCAGCTCATCTTCT+GGG | 0.368462 | 3.2:+73495703 | MS.gene79112:CDS |
| CTGGAGCGGAATTATCTAGA+AGG | 0.413522 | 3.2:+73495629 | MS.gene79112:CDS |
| TGCCGTATTCCATTAACAAA+AGG | 0.445652 | 3.2:+73495483 | MS.gene79112:CDS |
| GAGAATAGTGGAGGCGTTGG+AGG | 0.455797 | 3.2:+73495452 | MS.gene79112:CDS |
| TTCAAGCAGATACTCTTCCT+TGG | 0.459845 | 3.2:+73495276 | MS.gene79112:CDS |
| CAAAATTCACGATCAAAGTC+TGG | 0.467189 | 3.2:+73495732 | MS.gene79112:CDS |
| TCAAAGTCTGGCTGACCTGC+AGG | 0.480292 | 3.2:+73495744 | MS.gene79112:CDS |
| GATGAGAATAGTGGAGGCGT+TGG | 0.481773 | 3.2:+73495449 | MS.gene79112:CDS |
| GCCGTATTCCATTAACAAAA+GGG | 0.485365 | 3.2:+73495484 | MS.gene79112:CDS |
| CAGAGACAATGACTTCATCT+TGG | 0.494474 | 3.2:-73495104 | None:intergenic |
| GTGGAGGCGTTGGAGGGAAA+AGG | 0.503729 | 3.2:+73495459 | MS.gene79112:CDS |
| TTCTACCTGTCTAGTTATTG+TGG | 0.512168 | 3.2:-73495532 | None:intergenic |
| GATTATGATGGCTGAGGACA+TGG | 0.520825 | 3.2:+73495996 | MS.gene79112:CDS |
| TGTTTCAGTCTTTGAATCCA+AGG | 0.521920 | 3.2:-73495293 | None:intergenic |
| ACAGGTATTGCAGCAGCAGA+TGG | 0.527438 | 3.2:+73495921 | MS.gene79112:intron |
| GGTAGAATCTTACCTCCTGC+AGG | 0.538215 | 3.2:-73495759 | None:intergenic |
| ACTAGCCACAATAACTAGAC+AGG | 0.539256 | 3.2:+73495527 | MS.gene79112:CDS |
| AGAATAGTGGAGGCGTTGGA+GGG | 0.552709 | 3.2:+73495453 | MS.gene79112:CDS |
| TGTTCTGAAGCGAGACGCAC+GGG | 0.561337 | 3.2:+73495966 | MS.gene79112:CDS |
| AACACTGCCAAGTCTTGCTA+TGG | 0.563127 | 3.2:-73495947 | None:intergenic |
| GTGTTCTGAAGCGAGACGCA+CGG | 0.568554 | 3.2:+73495965 | MS.gene79112:CDS |
| ATTGAAGGTGATGAGAATAG+TGG | 0.583298 | 3.2:+73495440 | MS.gene79112:intron |
| ACGGGATATGGAGATTATGA+TGG | 0.590444 | 3.2:+73495984 | MS.gene79112:CDS |
| TATGGAGATTATGATGGCTG+AGG | 0.590797 | 3.2:+73495990 | MS.gene79112:CDS |
| ATGGATGCCATAGCAAGACT+TGG | 0.599180 | 3.2:+73495940 | MS.gene79112:CDS |
| ATTATGATGGCTGAGGACAT+GGG | 0.618742 | 3.2:+73495997 | MS.gene79112:CDS |
| CCGTATTCCATTAACAAAAG+GGG | 0.630334 | 3.2:+73495485 | MS.gene79112:CDS |
| TGGAGCGGAATTATCTAGAA+GGG | 0.631061 | 3.2:+73495630 | MS.gene79112:CDS |
| TCAGCTGAAAGGATCTGGAG+CGG | 0.638114 | 3.2:+73495615 | MS.gene79112:intron |
| GAAGGTGATGAGAATAGTGG+AGG | 0.650075 | 3.2:+73495443 | MS.gene79112:intron |
| ACTCGGCGTATCTCGTGTCA+AGG | 0.651033 | 3.2:+73495663 | MS.gene79112:CDS |
| AGAAGGGTACAAAATCTACT+CGG | 0.654843 | 3.2:+73495646 | MS.gene79112:CDS |
| AAGTCTGGCTGACCTGCAGG+AGG | 0.657558 | 3.2:+73495747 | MS.gene79112:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACATATGATTAGACATAATG+AGG | - | chr3.2:73495416-73495435 | None:intergenic | 25.0% |
| ! | ATATGATTAGACATAATGAG+GGG | - | chr3.2:73495414-73495433 | None:intergenic | 25.0% |
| ! | CATATGATTAGACATAATGA+GGG | - | chr3.2:73495415-73495434 | None:intergenic | 25.0% |
| !!! | ATACCATTTTAACATTGCTT+TGG | - | chr3.2:73495152-73495171 | None:intergenic | 25.0% |
| !!! | TACCATTTTAACATTGCTTT+GGG | - | chr3.2:73495151-73495170 | None:intergenic | 25.0% |
| AACCCAAAGCAATGTTAAAA+TGG | + | chr3.2:73495146-73495165 | MS.gene79112:CDS | 30.0% | |
| AAGGCTAATAGCAATGAATT+TGG | + | chr3.2:73495682-73495701 | MS.gene79112:CDS | 30.0% | |
| GAAGAACTCAATGTTAATGA+TGG | + | chr3.2:73495884-73495903 | MS.gene79112:intron | 30.0% | |
| TAAACCTCATATTGCCATAT+TGG | - | chr3.2:73495783-73495802 | None:intergenic | 30.0% | |
| TAATCATATGTCGTGATTGA+AGG | + | chr3.2:73495425-73495444 | MS.gene79112:intron | 30.0% | |
| TATCTGCTTGAAAATGTCTT+TGG | - | chr3.2:73495268-73495287 | None:intergenic | 30.0% | |
| TTAGCATAAAAAACCAGTAC+CGG | - | chr3.2:73495210-73495229 | None:intergenic | 30.0% | |
| ! | AAAATGTCTTTTCAGCTGAA+AGG | + | chr3.2:73495604-73495623 | MS.gene79112:intron | 30.0% |
| ! | CATTAAATGTCCATTTTCTG+AGG | - | chr3.2:73496025-73496044 | None:intergenic | 30.0% |
| !! | AGCAACGTATTTTAAGAGTA+TGG | + | chr3.2:73495333-73495352 | MS.gene79112:intron | 30.0% |
| ATTGAAGGTGATGAGAATAG+TGG | + | chr3.2:73495440-73495459 | MS.gene79112:intron | 35.0% | |
| CAAAATTCACGATCAAAGTC+TGG | + | chr3.2:73495732-73495751 | MS.gene79112:CDS | 35.0% | |
| CATCAGAGAATCGAAAAGAT+AGG | - | chr3.2:73495233-73495252 | None:intergenic | 35.0% | |
| CCGTATTCCATTAACAAAAG+GGG | + | chr3.2:73495485-73495504 | MS.gene79112:CDS | 35.0% | |
| GAGGTAAGATTCTACCAATA+TGG | + | chr3.2:73495766-73495785 | MS.gene79112:intron | 35.0% | |
| GCCGTATTCCATTAACAAAA+GGG | + | chr3.2:73495484-73495503 | MS.gene79112:CDS | 35.0% | |
| TCCCAAGAAAAATGTTGAAC+CGG | + | chr3.2:73495188-73495207 | MS.gene79112:intron | 35.0% | |
| TCTACCAATATGGCAATATG+AGG | + | chr3.2:73495776-73495795 | MS.gene79112:intron | 35.0% | |
| TGCCGTATTCCATTAACAAA+AGG | + | chr3.2:73495483-73495502 | MS.gene79112:CDS | 35.0% | |
| TGTTTCAGTCTTTGAATCCA+AGG | - | chr3.2:73495296-73495315 | None:intergenic | 35.0% | |
| TTCTACCTGTCTAGTTATTG+TGG | - | chr3.2:73495535-73495554 | None:intergenic | 35.0% | |
| ! | AGAAGGGTACAAAATCTACT+CGG | + | chr3.2:73495646-73495665 | MS.gene79112:CDS | 35.0% |
| ! | CAGACTTTGATCGTGAATTT+TGG | - | chr3.2:73495734-73495753 | None:intergenic | 35.0% |
| ! | CCCCTTTTGTTAATGGAATA+CGG | - | chr3.2:73495488-73495507 | None:intergenic | 35.0% |
| ! | CTATCTTTTCGATTCTCTGA+TGG | + | chr3.2:73495231-73495250 | MS.gene79112:intron | 35.0% |
| !! | ACCGGTTCAACATTTTTCTT+GGG | - | chr3.2:73495192-73495211 | None:intergenic | 35.0% |
| !!! | TACCGGTTCAACATTTTTCT+TGG | - | chr3.2:73495193-73495212 | None:intergenic | 35.0% |
| ACGGGATATGGAGATTATGA+TGG | + | chr3.2:73495984-73496003 | MS.gene79112:CDS | 40.0% | |
| ACTAGCCACAATAACTAGAC+AGG | + | chr3.2:73495527-73495546 | MS.gene79112:CDS | 40.0% | |
| ATGGTCTACTTTGTGAGAAC+AGG | + | chr3.2:73495903-73495922 | MS.gene79112:intron | 40.0% | |
| ATTATGATGGCTGAGGACAT+GGG | + | chr3.2:73495997-73496016 | MS.gene79112:CDS | 40.0% | |
| CAGAGACAATGACTTCATCT+TGG | - | chr3.2:73495107-73495126 | None:intergenic | 40.0% | |
| GAAAAATGTTGAACCGGTAC+TGG | + | chr3.2:73495194-73495213 | MS.gene79112:intron | 40.0% | |
| TATGGAGATTATGATGGCTG+AGG | + | chr3.2:73495990-73496009 | MS.gene79112:CDS | 40.0% | |
| TGGAGCGGAATTATCTAGAA+GGG | + | chr3.2:73495630-73495649 | MS.gene79112:CDS | 40.0% | |
| TTCAAGCAGATACTCTTCCT+TGG | + | chr3.2:73495276-73495295 | MS.gene79112:CDS | 40.0% | |
| ! | AACATTTTTCTTGGGAGAGC+AGG | - | chr3.2:73495184-73495203 | None:intergenic | 40.0% |
| ! | TCAGCTTCCCCTTTTGTTAA+TGG | - | chr3.2:73495495-73495514 | None:intergenic | 40.0% |
| ! | TCTTTTCAGCTGAAAGGATC+TGG | + | chr3.2:73495610-73495629 | MS.gene79112:intron | 40.0% |
| AACACTGCCAAGTCTTGCTA+TGG | - | chr3.2:73495950-73495969 | None:intergenic | 45.0% | |
| ATGGATGCCATAGCAAGACT+TGG | + | chr3.2:73495940-73495959 | MS.gene79112:CDS | 45.0% | |
| CTACTTCCTGTTCTGTTGCT+TGG | + | chr3.2:73495567-73495586 | MS.gene79112:intron | 45.0% | |
| CTGGAGCGGAATTATCTAGA+AGG | + | chr3.2:73495629-73495648 | MS.gene79112:CDS | 45.0% | |
| GAAGGTGATGAGAATAGTGG+AGG | + | chr3.2:73495443-73495462 | MS.gene79112:intron | 45.0% | |
| GACATGGGAACCTCAGAAAA+TGG | + | chr3.2:73496012-73496031 | MS.gene79112:CDS | 45.0% | |
| GATTATGATGGCTGAGGACA+TGG | + | chr3.2:73495996-73496015 | MS.gene79112:CDS | 45.0% | |
| GGTAACTCAGCTCATCTTCT+GGG | + | chr3.2:73495703-73495722 | MS.gene79112:CDS | 45.0% | |
| TGGTAACTCAGCTCATCTTC+TGG | + | chr3.2:73495702-73495721 | MS.gene79112:CDS | 45.0% | |
| TTCCATCCAAGCAACAGAAC+AGG | - | chr3.2:73495576-73495595 | None:intergenic | 45.0% | |
| ! | TTCCTGTTCTGTTGCTTGGA+TGG | + | chr3.2:73495571-73495590 | MS.gene79112:intron | 45.0% |
| GGTAGAATCTTACCTCCTGC+AGG | - | chr3.2:73495762-73495781 | None:intergenic | 50.0% | |
| TCAGCTGAAAGGATCTGGAG+CGG | + | chr3.2:73495615-73495634 | MS.gene79112:intron | 50.0% | |
| TTCTTGGGAGAGCAGGAGAA+AGG | - | chr3.2:73495177-73495196 | None:intergenic | 50.0% | |
| ! | ACAGGTATTGCAGCAGCAGA+TGG | + | chr3.2:73495921-73495940 | MS.gene79112:intron | 50.0% |
| ! | GATGAGAATAGTGGAGGCGT+TGG | + | chr3.2:73495449-73495468 | MS.gene79112:CDS | 50.0% |
| !! | AGAATAGTGGAGGCGTTGGA+GGG | + | chr3.2:73495453-73495472 | MS.gene79112:CDS | 50.0% |
| ACTCGGCGTATCTCGTGTCA+AGG | + | chr3.2:73495663-73495682 | MS.gene79112:CDS | 55.0% | |
| TCAAAGTCTGGCTGACCTGC+AGG | + | chr3.2:73495744-73495763 | MS.gene79112:CDS | 55.0% | |
| !! | GAGAATAGTGGAGGCGTTGG+AGG | + | chr3.2:73495452-73495471 | MS.gene79112:CDS | 55.0% |
| !! | GTGTTCTGAAGCGAGACGCA+CGG | + | chr3.2:73495965-73495984 | MS.gene79112:CDS | 55.0% |
| !! | TGTTCTGAAGCGAGACGCAC+GGG | + | chr3.2:73495966-73495985 | MS.gene79112:CDS | 55.0% |
| AAGTCTGGCTGACCTGCAGG+AGG | + | chr3.2:73495747-73495766 | MS.gene79112:CDS | 60.0% | |
| GAAGCGAGACGCACGGGATA+TGG | + | chr3.2:73495972-73495991 | MS.gene79112:CDS | 60.0% | |
| !! | GTGGAGGCGTTGGAGGGAAA+AGG | + | chr3.2:73495459-73495478 | MS.gene79112:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 73495102 | 73496047 | 73495102 | ID=MS.gene79112 |
| chr3.2 | mRNA | 73495102 | 73496047 | 73495102 | ID=MS.gene79112.t1;Parent=MS.gene79112 |
| chr3.2 | exon | 73495102 | 73495167 | 73495102 | ID=MS.gene79112.t1.exon1;Parent=MS.gene79112.t1 |
| chr3.2 | CDS | 73495102 | 73495167 | 73495102 | ID=cds.MS.gene79112.t1;Parent=MS.gene79112.t1 |
| chr3.2 | exon | 73495263 | 73495349 | 73495263 | ID=MS.gene79112.t1.exon2;Parent=MS.gene79112.t1 |
| chr3.2 | CDS | 73495263 | 73495349 | 73495263 | ID=cds.MS.gene79112.t1;Parent=MS.gene79112.t1 |
| chr3.2 | exon | 73495447 | 73495548 | 73495447 | ID=MS.gene79112.t1.exon3;Parent=MS.gene79112.t1 |
| chr3.2 | CDS | 73495447 | 73495548 | 73495447 | ID=cds.MS.gene79112.t1;Parent=MS.gene79112.t1 |
| chr3.2 | exon | 73495619 | 73495768 | 73495619 | ID=MS.gene79112.t1.exon4;Parent=MS.gene79112.t1 |
| chr3.2 | CDS | 73495619 | 73495768 | 73495619 | ID=cds.MS.gene79112.t1;Parent=MS.gene79112.t1 |
| chr3.2 | exon | 73495925 | 73496047 | 73495925 | ID=MS.gene79112.t1.exon5;Parent=MS.gene79112.t1 |
| chr3.2 | CDS | 73495925 | 73496047 | 73495925 | ID=cds.MS.gene79112.t1;Parent=MS.gene79112.t1 |
| Gene Sequence |
| Protein sequence |
